pycom13g00170

recombinational repair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003214
Physical Location & Seq
Forward (+)
109432 .. 112974
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1224 bp
ATGTTGCGTCGAACAAAAGGAACATTGATCGATGGGCAACCTATAATTGAATTACCACCAAAAACAATAAACTTGAGTAAAGTGGAGTTCTCGTCTGAGGAGCGGGCATTTTATACCAAGCTAGAAGCCGATTCACGCACTAAGTTCAAGGCATATGCCGCTGCTGGCACAGTAAATCAAAATTATGCAAATATTCTTTTGATGCTTTTGCGCCTCCGGCAAGCTTGTGACCACCCTCAGCTTGTTAAAGCGTATGACTCAGACTGTGTTGGGAAAGATTCTGTGCAAATGGCAAGGCGACTCCCAAAGGATATGATATCCCATCTTTTGCATCTTCTGGAAACTTCATTGGCTATTTGTCGTGTATGCAAAGATCCACCTGAAGACCCTGTTGTTACTATGTGTGGCCATGTATTCTGTTATCAGTGTGTTTCAGAATATTTGACGGGTGATGACAATACATGCCCTGCTGCTGAATGTAAAGAACAAGTTGGTTCTGATGTTGTTTTCTCCAAATCCACTCTCATAAGTTGCTTCTCCAATAATCTTGATGGTACTCCGACGAATTCTGAGTTGGGCGAGAAATCAATAGTGTTACAGAATGAGTATAGTTCATCTAAAGTCAGAGCTATTATTGAGATTTTGCTTTCACATTTGGAACATAACTCTGCTGGAAGCAATGGAGACCCTGCTTTTGGAACTGAAATCACAGATATTAGATATTCTGGCGTTTCAAGCTCACCAAATAGTGGACCAATAAAAACGATTATTTTTTCCCAGTGGACTGGCATGTTGGATTTAGTGGAAACATCATTGAACGAGTATTGTATTCAGTACAGAAGGCTTGATGGGACAATGAGTCTGGCATCAAGAGACAGGGGTGTCAAAGATTTTAACACTGATCCTGAGATCACTGTTATGCTCATGTCACTAAAGGCTGGAAACCTTGGTCTAAACATGGTTGCTGCATGTCATGTTATCCTTTTGGACCTGTGGTGGAATCCAACCACTGAAGATCAAGCTATTGATCGGGCACATAGAATTGGACAGACCCGACCTGTTACTGTAACACGTCTCACGATTAAGGATACAGTGGAGGACAGGATATTAGCTTTACAGGAAGAAAAGAGAAAAATGGTGGCATCTGCTTTTGGTGAAGATCATGGTGGGGGCTCTGCTACACGTCTGACTGTTGAGGATCTGAGATACCTATTTATGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000785 GO:0000790 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006304 GO:0006305 GO:0006306 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007154 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009059 GO:0009292 GO:0009294 GO:0009605 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0009991 GO:0010468 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016458 GO:0016462 GO:0016567 GO:0016740 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019538 GO:0019787 GO:0022402 GO:0030162 GO:0031047 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031668 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032269 GO:0032392 GO:0032434 GO:0032435 GO:0032446 GO:0032508 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042176 GO:0042177 GO:0042262 GO:0042623 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0044764 GO:0044786 GO:0045003 GO:0045005 GO:0045861 GO:0046483 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0051704 GO:0051716 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070035 GO:0070647 GO:0071103 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0140083 GO:0140096 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901799 GO:1902298 GO:1902969 GO:1903047 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:1990505 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
ko04918 map04918
Pfam Domains
Protein Families
Helicase_C SNF2_N zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

408

Amino Acids

45.16

Weight (kDa)

5.57

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 1 - 81 3.2e-06 SNF2-related domain
zf-C3HC4_3 PF13920 119 - 163 3.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 120 - 156 2.2e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 120 - 156 6.7e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 120 - 156 7.6e-07 RING-type zinc-finger
zf-RING_5 PF14634 120 - 161 4.7e-06 zinc-RING finger domain
Helicase_C PF00271 254 - 349 1.9e-19 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 881
AccB7I CCANNNNNTGG 1 cut(s) 749
AccBSI CCGCTC 1 cut(s) 103
AciI CCGC 2 cut(s) 103, 159
AclWI GGATC 3 cut(s) 368, 896, 1206
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 2 cut(s) 402, 1032
AfaI GTAC 2 cut(s) 556, 836
AfiI CCNNNNNNNGG 3 cut(s) 695, 749, 1216
AflIII ACRYGT 2 cut(s) 1070, 1181
AgsI TTSAA 4 cut(s) 50, 148, 735, 817
AhdI GACNNNNNGTC 1 cut(s) 858
AjiI CACGTC 2 cut(s) 1073, 1184
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AluBI AGCT 7 cut(s) 121, 224, 241, 629, 738, 1022, 1112
AluI AGCT 7 cut(s) 121, 224, 241, 629, 738, 1022, 1112
Alw26I GTCTC 3 cut(s) 678, 867, 1079
AlwI GGATC 3 cut(s) 368, 896, 1206
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 3 cut(s) 161, 470, 965
ApoI RAATTY 1 cut(s) 565
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 2 cut(s) 752, 988
AsuHPI GGTGA 3 cut(s) 461, 732, 1166
AvaII GGWCC 2 cut(s) 752, 988
BaeGI GKGCMC 1 cut(s) 1036
BalI TGGCCA 1 cut(s) 408
BanII GRGCYC 1 cut(s) 1175
BbsI GAAGAC 1 cut(s) 390
BbvCI CCTCAGC 1 cut(s) 237
BbvI GCAGC 3 cut(s) 148, 457, 952
BccI CCATC 4 cut(s) 26, 330, 545, 842
BciVI GTATCC 1 cut(s) 1081
BcoDI GTCTC 3 cut(s) 678, 867, 1079
BfaI CTAG 1 cut(s) 122
BfuI GTATCC 1 cut(s) 1081
BisI GCNGC 4 cut(s) 159, 162, 471, 966
BlsI GCNGC 4 cut(s) 160, 163, 472, 967
Bme18I GGWCC 2 cut(s) 752, 988
BmeRI GACNNNNNGTC 1 cut(s) 858
BmgBI CACGTC 2 cut(s) 1073, 1184
BmgT120I GGNCC 2 cut(s) 752, 988
BmrI ACTGGG 1 cut(s) 772
BmsI GCATC 4 cut(s) 192, 340, 875, 1151
BmuI ACTGGG 1 cut(s) 772
BpiI GAAGAC 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 678
BsaJI CCNNGG 1 cut(s) 946
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 3 cut(s) 695, 749, 1216
Bse1I ACTGG 2 cut(s) 778, 790
Bse3DI GCAATG 1 cut(s) 685
BseCI ATCGAT 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 946
BseLI CCNNNNNNNGG 3 cut(s) 695, 749, 1216
BseMI GCAATG 1 cut(s) 685
BseMII CTCAG 6 cut(s) 87, 251, 273, 561, 897, 1193
BseNI ACTGG 2 cut(s) 778, 790
BseRI GAGGAG 1 cut(s) 113
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 3 cut(s) 148, 457, 952
BshFI GGCC 1 cut(s) 408
BshVI ATCGAT 1 cut(s) 30
BsiSI CCGG 1 cut(s) 217
BslFI GGGAC 1 cut(s) 865
BslI CCNNNNNNNGG 3 cut(s) 695, 749, 1216
BsmAI GTCTC 3 cut(s) 678, 867, 1079
BsmBI CGTCTC 1 cut(s) 1079
BsmFI GGGAC 1 cut(s) 865
BsnI GGCC 1 cut(s) 408
Bso31I GGTCTC 1 cut(s) 678
Bsp1286I GDGCHC 2 cut(s) 1036, 1175
Bsp143I GATC 8 cut(s) 27, 373, 901, 909, 1015, 1027, 1159, 1198
BspACI CCGC 2 cut(s) 103, 159
BspANI GGCC 1 cut(s) 408
BspCNI CTCAG 6 cut(s) 88, 250, 272, 562, 898, 1194
BspDI ATCGAT 1 cut(s) 30
BspPI GGATC 3 cut(s) 368, 896, 1206
BspTNI GGTCTC 1 cut(s) 678
BsrBI CCGCTC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 685
BsrI ACTGG 2 cut(s) 778, 790
BssECI CCNNGG 1 cut(s) 946
BssMI GATC 8 cut(s) 27, 373, 901, 909, 1015, 1027, 1159, 1198
BssT1I CCWWGG 1 cut(s) 946
Bst4CI ACNGT 6 cut(s) 172, 266, 916, 1066, 1093, 1192
BstC8I GCNNGC 3 cut(s) 105, 166, 222
BstDEI CTNAG 7 cut(s) 96, 141, 237, 259, 570, 906, 1202
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 8 cut(s) 30, 376, 904, 912, 1018, 1030, 1162, 1201
BstMAI GTCTC 3 cut(s) 678, 867, 1079
BstMBI GATC 8 cut(s) 27, 373, 901, 909, 1015, 1027, 1159, 1198
BstMWI GCNNNNNNNGC 3 cut(s) 158, 217, 735
BstNSI RCATGY 3 cut(s) 465, 793, 972
BstSLI GKGCMC 1 cut(s) 1036
BstV1I GCAGC 3 cut(s) 148, 457, 952
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 2 cut(s) 373, 1198
BstXI CCANNNNNNTGG 1 cut(s) 785
BstYI RGATCY 2 cut(s) 373, 1198
Bsu15I ATCGAT 1 cut(s) 30
BsuI GTATCC 1 cut(s) 1081
BsuRI GGCC 1 cut(s) 408
BsuTUI ATCGAT 1 cut(s) 30
BtrI CACGTC 2 cut(s) 1073, 1184
BtsIMutI CAGTG 6 cut(s) 431, 785, 897, 912, 1008, 1098
Cac8I GCNNGC 3 cut(s) 105, 166, 222
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 2 cut(s) 752, 988
ClaI ATCGAT 1 cut(s) 30
Csp6I GTAC 2 cut(s) 555, 835
CviAII CATG 8 cut(s) 410, 462, 790, 925, 958, 969, 974, 1163
CviQI GTAC 2 cut(s) 555, 835
DdeI CTNAG 7 cut(s) 96, 141, 237, 259, 570, 906, 1202
DpnI GATC 8 cut(s) 29, 375, 903, 911, 1017, 1029, 1161, 1200
DpnII GATC 8 cut(s) 27, 373, 901, 909, 1015, 1027, 1159, 1198
DrdI GACNNNNNNGTC 1 cut(s) 881
DriI GACNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 881
EaeI YGGCCR 1 cut(s) 406
Eam1105I GACNNNNNGTC 1 cut(s) 858
Eco130I CCWWGG 1 cut(s) 946
Eco24I GRGCYC 1 cut(s) 1175
Eco31I GGTCTC 1 cut(s) 678
Eco32I GATATC 1 cut(s) 318
Eco47I GGWCC 2 cut(s) 752, 988
Eco57I CTGAAG 2 cut(s) 402, 1032
EcoRI GAATTC 1 cut(s) 565
EcoRV GATATC 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 946
EcoT38I GRGCYC 1 cut(s) 1175
ErhI CCWWGG 1 cut(s) 946
Esp3I CGTCTC 1 cut(s) 1079
FaeI CATG 8 cut(s) 413, 465, 793, 928, 961, 972, 977, 1166
FaqI GGGAC 1 cut(s) 865
FatI CATG 8 cut(s) 409, 461, 789, 924, 957, 968, 973, 1162
FauI CCCGC 1 cut(s) 96
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 4 cut(s) 159, 162, 471, 966
FriOI GRGCYC 1 cut(s) 1175
Fsp4HI GCNGC 4 cut(s) 159, 162, 471, 966
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 212
GluI GCNGC 4 cut(s) 159, 162, 471, 966
HaeIII GGCC 1 cut(s) 408
HapII CCGG 1 cut(s) 217
HhaI GCGC 1 cut(s) 213
Hin1II CATG 8 cut(s) 413, 465, 793, 928, 961, 972, 977, 1166
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 222
HinfI GANTC 6 cut(s) 131, 257, 278, 300, 859, 1000
HpaII CCGG 1 cut(s) 217
HphI GGTGA 3 cut(s) 461, 732, 1166
Hpy166II GTNNAC 2 cut(s) 752, 783
Hpy188I TCNGA 9 cut(s) 97, 262, 436, 499, 561, 571, 626, 1188, 1203
Hpy188III TCNNGA 5 cut(s) 338, 548, 870, 905, 1078
Hpy8I GTNNAC 2 cut(s) 752, 783
Hpy99I CGWCG 2 cut(s) 12, 565
HpyAV CCTTC 1 cut(s) 834
HpyCH4III ACNGT 6 cut(s) 172, 266, 916, 1066, 1093, 1192
HpyCH4IV ACGT 2 cut(s) 1072, 1183
HpyCH4V TGCA 5 cut(s) 188, 286, 331, 369, 968
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 217, 735
HpyF3I CTNAG 7 cut(s) 96, 141, 237, 259, 570, 906, 1202
HpySE526I ACGT 2 cut(s) 1072, 1183
Hsp92II CATG 8 cut(s) 413, 465, 793, 928, 961, 972, 977, 1166
HspAI GCGC 1 cut(s) 211
Kzo9I GATC 8 cut(s) 27, 373, 901, 909, 1015, 1027, 1159, 1198
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 3 cut(s) 148, 457, 952
LweI GCATC 4 cut(s) 192, 340, 875, 1151
MaeI CTAG 1 cut(s) 122
MaeII ACGT 2 cut(s) 1072, 1183
MaeIII GTNAC 6 cut(s) 227, 394, 594, 927, 1060, 1066
MalI GATC 8 cut(s) 29, 375, 903, 911, 1017, 1029, 1161, 1200
MbiI CCGCTC 1 cut(s) 103
MboI GATC 8 cut(s) 27, 373, 901, 909, 1015, 1027, 1159, 1198
MboII GAAGA 5 cut(s) 326, 395, 1025, 1133, 1169
MflI RGATCY 2 cut(s) 373, 1198
MhlI GDGCHC 2 cut(s) 1036, 1175
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 5 cut(s) 45, 50, 181, 565, 1041
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 3 cut(s) 251, 294, 868
MmeI TCCRAC 3 cut(s) 584, 774, 1028
MnlI CCTC 5 cut(s) 91, 224, 246, 1090, 1189
Mox20I TGGCCA 1 cut(s) 408
MscI TGGCCA 1 cut(s) 408
MseI TTAA 3 cut(s) 246, 894, 1083
MslI CAYNNNNRTG 1 cut(s) 917
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 158, 217, 735
NdeI CATATG 1 cut(s) 154
NdeII GATC 8 cut(s) 27, 373, 901, 909, 1015, 1027, 1159, 1198
NlaIII CATG 8 cut(s) 413, 465, 793, 928, 961, 972, 977, 1166
NmuCI GTSAC 2 cut(s) 227, 927
NspI RCATGY 3 cut(s) 465, 793, 972
PfeI GAWTC 3 cut(s) 131, 278, 1000
PflMI CCANNNNNTGG 1 cut(s) 749
PkrI GCNGC 4 cut(s) 160, 163, 472, 967
PleI GAGTC 3 cut(s) 251, 294, 867
PpsI GAGTC 3 cut(s) 251, 294, 867
PspPI GGNCC 2 cut(s) 752, 988
PsuI RGATCY 2 cut(s) 373, 1198
RsaI GTAC 2 cut(s) 556, 836
RsaNI GTAC 2 cut(s) 555, 835
RseI CAYNNNNRTG 1 cut(s) 917
SaqAI TTAA 3 cut(s) 246, 894, 1083
SatI GCNGC 4 cut(s) 159, 162, 471, 966
Sau3AI GATC 8 cut(s) 27, 373, 901, 909, 1015, 1027, 1159, 1198
Sau96I GGNCC 2 cut(s) 752, 988
SchI GAGTC 3 cut(s) 251, 294, 868
SduI GDGCHC 2 cut(s) 1036, 1175
SfaNI GCATC 4 cut(s) 192, 340, 875, 1151
SinI GGWCC 2 cut(s) 752, 988
SmiMI CAYNNNNRTG 1 cut(s) 917
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 5 cut(s) 45, 50, 181, 565, 1041
SsiI CCGC 2 cut(s) 103, 159
SspI AATATT 2 cut(s) 193, 440
SspMI CTAG 1 cut(s) 122
StyI CCWWGG 1 cut(s) 946
TaaI ACNGT 6 cut(s) 172, 266, 916, 1066, 1093, 1192
TaiI ACGT 2 cut(s) 1075, 1186
TaqI TCGA 2 cut(s) 10, 30
TasI AATT 5 cut(s) 45, 50, 181, 565, 1041
TatI WGTACW 1 cut(s) 834
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 3 cut(s) 131, 278, 1000
Tru1I TTAA 3 cut(s) 246, 894, 1083
Tru9I TTAA 3 cut(s) 246, 894, 1083
TscAI CASTG 6 cut(s) 431, 785, 904, 919, 1015, 1098
TseFI GTSAC 2 cut(s) 227, 927
TseI GCWGC 3 cut(s) 161, 470, 965
Tsp45I GTSAC 2 cut(s) 227, 927
TspDTI ATGAA 2 cut(s) 336, 603
TspRI CASTG 6 cut(s) 431, 785, 904, 919, 1015, 1098
Van91I CCANNNNNTGG 1 cut(s) 749
VpaK11BI GGWCC 2 cut(s) 752, 988
XapI RAATTY 1 cut(s) 565
XceI RCATGY 3 cut(s) 465, 793, 972
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.