pycom13g02400

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005491
Physical Location & Seq
Forward (+)
1651269 .. 1654573
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1815 bp
ATGTCTGGGGAGATAGAAGAGCCTGTTGGCTTGAGCTTTCAGGCAGACTCTTTACACTCTGGTTCCATATCATTCGGGAGGTTTGAAAATGAAGTTTTATCTTGGGAAAAGAGGTCATCTTTCTCGCATAACAGATATGTTGAAGAGGTTGAAAAATGCTTGAAACCGGGTTCAGTTATTGAGAGGAAAGCTTACTTCGAAGCTCATTTCAAGAAGAAGGGTTTTCCCAAACCAAATTTAGTCGAGTGCTATAGCGGTTCAAATTATCAAGTCTGTGAAAATGATGTCTTGGAGAGTGATGCGTATGGAGAAGAATTTGAAGACGGAAATGAAGATAATGGTTGCGCTCATTTTGATGAGAACTCGGAGGGTTCAGAGTACCACGGAGACTTTGAAGTGAAAGAATGTGAAAGGGAAGATCGATCTGAAGTTTCATTGTCCGATCCTCAGAGGGCATCTGAGTTGAACGATGAACATATTCTGGTGGATATTAAAGATGATAATCCCGAGGAAACACATCAAACTGGAATAGGTCGTGACACGTTTTCTTCAAGTATTGATGAACCAGAGACCGATGTAAATGAGAATCATAATGGTGATGCTGTGAATGCTGATGAATCACATAATTCTGTTACTATGAACCCCAAGACTGCAGCAGATGAGGAAGTTAACATCACCATTGTGGAAAGTTTGCATAATTCTTCTTCAAAGTTGAAGGCTGGAAAAGAATCCAAAATTTCCAAGTCCAGAGTAAAATCTAAGGCCAGCATTTCTCCGGTTAAGAGAAGCATTTCTGCTGAGTCGTCTAAAGTTCCTATAAATAAATCTAATGCAAGAGAAAGAGAGGGTACACGAAGAACGGATAAAGAAAAGCTGTCATCAAAAACTGCAATTCCACCTACGCATTCTGTTCGCACGACTCCAAAATCAGAGGATTCTACAAAACTGAAGGGAAAATCTGCTCATGAGAGTCGAAGTGATAAAGAGTTCTTTGTTAAGAACCTTGGTGAACCTCAACCTCCTGCCGTCAAGCCTGAGTCCAGAGGACGCCAAACGGCAAACAGGCTTAACCAAAGAGTCAATGTAACCAAGTCTGATACAAGATCAAGTGTTGCAACTTTCAATTTCAAAAGTTCTGAACGAGCAGAAAGGAGGAAAGAGTTCTTCACGAAGATGGAAGAAAAAATGCATGCCAAGGAAGATGAGATGAATAAGATCCAAGCAAGAAAGCAGGAAAAAAACGAAGCTGAGATTAGACAATTCCGCAAAAGCCTCAACTTCAAAGCAGCACCAATGCCTTCTTTCTATCATTCAGCTGTGAATTCAGTGCATGATGGGAGCAAGGCTGCATCTACCAAATCCTCTAAAGTACAAAGCATGTCAACAAATCCAGGGAGTGGAGCTACCTCAAGATTGCCATCACATTCAGAGACAGGAAAGTCTAATGCTCCTACTGATAGTGATTCTGTAAACACAACCGATTTGCCCGAGGCCTCAAGAGAAACTAACTTCATGATGGCTGAGCCCTCTGAAGCTAGTTCAGTTTCTTCAACCACATTGAGCGGCAAAAACTTATCCCGAGATACAACAAAAGGTGAAGCCACGAGGACGAAAGAAAGAGAGAAGAATGCTAATACACAGAAACATCGATCATTAGAGAGCGGAAAAATTGCAAAAAGTCATAAAACTGAAGGGAAGCAGAAAGTGGGAGGCGAAAGAAATAGTAGAGAGATGGCAAGGAAGGGTGTGAAAGTCAGCGTCGGCTTTGGAAGCAGTTCAAGAATGGGTCATCTAACAGTTGGTGTTGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

605

Amino Acids

66.81

Weight (kDa)

6.71

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 374 - 441 1.2e-22 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1438
AccB7I CCANNNNNTGG 1 cut(s) 1397
AccBSI CCGCTC 2 cut(s) 1563, 1662
AciI CCGC 4 cut(s) 255, 1264, 1563, 1662
AclWI GGATC 2 cut(s) 437, 1210
AcsI RAATTY 4 cut(s) 235, 314, 735, 1321
AcuI CTGAAG 4 cut(s) 447, 968, 1551, 1710
AcyI GRCGYC 1 cut(s) 1048
AfaI GTAC 3 cut(s) 380, 850, 1371
AfiI CCNNNNNNNGG 1 cut(s) 1397
AflIII ACRYGT 1 cut(s) 540
AjnI CCWGG 1 cut(s) 1390
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AleI CACNNNNGTG 1 cut(s) 680
AluBI AGCT 8 cut(s) 36, 191, 203, 874, 1247, 1316, 1403, 1535
AluI AGCT 8 cut(s) 36, 191, 203, 874, 1247, 1316, 1403, 1535
Alw26I GTCTC 3 cut(s) 381, 563, 1424
AlwI GGATC 2 cut(s) 437, 1210
Ama87I CYCGRG 3 cut(s) 506, 1487, 1578
AoxI GGCC 2 cut(s) 762, 1491
ApeKI GCWGC 3 cut(s) 653, 1286, 1346
ApoI RAATTY 4 cut(s) 235, 314, 735, 1321
Asp700I GAANNNNTTC 4 cut(s) 477, 790, 1160, 1774
AspLEI GCGC 1 cut(s) 347
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 4 cut(s) 608, 667, 1019, 1607
AsuII TTCGAA 1 cut(s) 198
AvaI CYCGRG 3 cut(s) 506, 1487, 1578
BanII GRGCYC 1 cut(s) 1527
BauI CACGAG 1 cut(s) 1603
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 3 cut(s) 665, 1298, 1333
BccI CCATC 5 cut(s) 1168, 1328, 1426, 1510, 1726
BceAI ACGGC 2 cut(s) 1010, 1071
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BciT130I CCWGG 1 cut(s) 1392
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 3 cut(s) 381, 563, 1424
BfaI CTAG 1 cut(s) 1536
BfmI CTRYAG 2 cut(s) 250, 651
BisI GCNGC 4 cut(s) 654, 1287, 1347, 1564
BlpI GCTNAGC 1 cut(s) 1521
BlsI GCNGC 4 cut(s) 655, 1288, 1348, 1565
Bme1390I CCNGG 2 cut(s) 168, 1392
BmeT110I CYCGRG 3 cut(s) 506, 1487, 1578
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 2 cut(s) 168, 1392
BmsI GCATC 4 cut(s) 289, 464, 589, 1358
BpiI GAAGAC 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 1521
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 3 cut(s) 52, 1393, 1480
BpuMI CCSGG 1 cut(s) 168
Bsa29I ATCGAT 2 cut(s) 421, 1648
BsaBI GATNNNNATC 2 cut(s) 501, 1417
BsaHI GRCGYC 1 cut(s) 1048
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 6 cut(s) 382, 507, 1003, 1194, 1391, 1488
BsaWI WCCGGW 1 cut(s) 775
Bsc4I CCNNNNNNNGG 1 cut(s) 1397
Bse1I ACTGG 1 cut(s) 529
Bse8I GATNNNNATC 2 cut(s) 501, 1417
BseBI CCWGG 1 cut(s) 1392
BseCI ATCGAT 2 cut(s) 421, 1648
BseDI CCNNGG 6 cut(s) 382, 507, 1003, 1194, 1391, 1488
BseJI GATNNNNATC 2 cut(s) 501, 1417
BseLI CCNNNNNNNGG 1 cut(s) 1397
BseMII CTCAG 6 cut(s) 450, 461, 789, 1026, 1239, 1512
BseNI ACTGG 1 cut(s) 529
BseXI GCAGC 3 cut(s) 665, 1298, 1333
BshFI GGCC 2 cut(s) 764, 1493
BshVI ATCGAT 2 cut(s) 421, 1648
BsiHKCI CYCGRG 3 cut(s) 506, 1487, 1578
BsiSI CCGG 2 cut(s) 167, 776
BslI CCNNNNNNNGG 1 cut(s) 1397
BsmAI GTCTC 3 cut(s) 381, 563, 1424
BsmI GAATGC 3 cut(s) 613, 904, 1633
BsnI GGCC 2 cut(s) 764, 1493
Bso31I GGTCTC 1 cut(s) 563
BsoBI CYCGRG 3 cut(s) 506, 1487, 1578
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 1527
Bsp143I GATC 6 cut(s) 418, 422, 442, 1103, 1215, 1649
Bsp1720I GCTNAGC 1 cut(s) 1521
BspACI CCGC 4 cut(s) 255, 1264, 1563, 1662
BspANI GGCC 2 cut(s) 764, 1493
BspCNI CTCAG 6 cut(s) 451, 460, 790, 1027, 1240, 1513
BspDI ATCGAT 2 cut(s) 421, 1648
BspHI TCATGA 3 cut(s) 964, 1512, 1811
BspLI GGNNCC 1 cut(s) 64
BspMAI CTGCAG 1 cut(s) 655
BspPI GGATC 2 cut(s) 437, 1210
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 198
BspTNI GGTCTC 1 cut(s) 563
BsrBI CCGCTC 2 cut(s) 1563, 1662
BsrI ACTGG 1 cut(s) 529
BssECI CCNNGG 6 cut(s) 382, 507, 1003, 1194, 1391, 1488
BssMI GATC 6 cut(s) 418, 422, 442, 1103, 1215, 1649
BssNI GRCGYC 1 cut(s) 1048
BssSI CACGAG 1 cut(s) 1603
BssT1I CCWWGG 2 cut(s) 1003, 1194
Bst2BI CACGAG 1 cut(s) 1603
Bst2UI CCWGG 1 cut(s) 1392
Bst4CI ACNGT 1 cut(s) 1798
Bst6I CTCTTC 2 cut(s) 12, 138
BstACI GRCGYC 1 cut(s) 1048
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 766, 1191
BstDEI CTNAG 7 cut(s) 447, 459, 759, 798, 1035, 1248, 1521
BstDSI CCRYGG 1 cut(s) 382
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 6 cut(s) 421, 425, 445, 1106, 1218, 1652
BstMAI GTCTC 3 cut(s) 381, 563, 1424
BstMBI GATC 6 cut(s) 418, 422, 442, 1103, 1215, 1649
BstMWI GCNNNNNNNGC 2 cut(s) 608, 1770
BstNI CCWGG 1 cut(s) 1392
BstNSI RCATGY 2 cut(s) 1193, 1381
BstSCI CCNGG 2 cut(s) 166, 1390
BstSFI CTRYAG 2 cut(s) 250, 651
BstV1I GCAGC 3 cut(s) 665, 1298, 1333
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 1215
BstYI RGATCY 1 cut(s) 1215
Bsu15I ATCGAT 2 cut(s) 421, 1648
BsuRI GGCC 2 cut(s) 764, 1493
BsuTUI ATCGAT 2 cut(s) 421, 1648
BtgI CCRYGG 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 1332
Cac8I GCNNGC 2 cut(s) 766, 1191
CciI TCATGA 3 cut(s) 964, 1512, 1811
CfoI GCGC 1 cut(s) 347
ClaI ATCGAT 2 cut(s) 421, 1648
CseI GACGC 2 cut(s) 1056, 1747
Csp6I GTAC 3 cut(s) 379, 849, 1370
CviAII CATG 6 cut(s) 965, 1190, 1331, 1378, 1513, 1812
CviQI GTAC 3 cut(s) 379, 849, 1370
DdeI CTNAG 7 cut(s) 447, 459, 759, 798, 1035, 1248, 1521
DpnI GATC 6 cut(s) 420, 424, 444, 1105, 1217, 1651
DpnII GATC 6 cut(s) 418, 422, 442, 1103, 1215, 1649
DrdI GACNNNNNNGTC 1 cut(s) 1438
DseDI GACNNNNNNGTC 1 cut(s) 1438
Eam1104I CTCTTC 2 cut(s) 12, 138
EarI CTCTTC 2 cut(s) 12, 138
Eco130I CCWWGG 2 cut(s) 1003, 1194
Eco147I AGGCCT 1 cut(s) 1493
Eco24I GRGCYC 1 cut(s) 1527
Eco31I GGTCTC 1 cut(s) 563
Eco57I CTGAAG 4 cut(s) 447, 968, 1551, 1710
Eco88I CYCGRG 3 cut(s) 506, 1487, 1578
EcoRI GAATTC 1 cut(s) 1321
EcoRII CCWGG 1 cut(s) 1390
EcoT14I CCWWGG 2 cut(s) 1003, 1194
EcoT22I ATGCAT 1 cut(s) 1191
EcoT38I GRGCYC 1 cut(s) 1527
ErhI CCWWGG 2 cut(s) 1003, 1194
FaeI CATG 6 cut(s) 968, 1193, 1334, 1381, 1516, 1815
FatI CATG 6 cut(s) 964, 1189, 1330, 1377, 1512, 1811
Fnu4HI GCNGC 4 cut(s) 654, 1287, 1347, 1564
FriOI GRGCYC 1 cut(s) 1527
Fsp4HI GCNGC 4 cut(s) 654, 1287, 1347, 1564
FspBI CTAG 1 cut(s) 1536
GlaI GCGC 1 cut(s) 346
GluI GCNGC 4 cut(s) 654, 1287, 1347, 1564
HaeIII GGCC 2 cut(s) 764, 1493
HapII CCGG 2 cut(s) 167, 776
HgaI GACGC 2 cut(s) 1056, 1747
HhaI GCGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 1048
Hin1II CATG 6 cut(s) 968, 1193, 1334, 1381, 1516, 1815
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HincII GTYRAC 2 cut(s) 670, 1383
HindII GTYRAC 2 cut(s) 670, 1383
HindIII AAGCTT 1 cut(s) 189
HpaI GTTAAC 1 cut(s) 670
HpaII CCGG 2 cut(s) 167, 776
HphI GGTGA 4 cut(s) 608, 667, 1019, 1607
Hpy166II GTNNAC 5 cut(s) 670, 851, 1010, 1383, 1471
Hpy8I GTNNAC 5 cut(s) 670, 851, 1010, 1383, 1471
Hpy99I CGWCG 1 cut(s) 1763
HpyAV CCTTC 6 cut(s) 211, 709, 943, 1308, 1685, 1735
HpyCH4III ACNGT 1 cut(s) 1798
HpyCH4IV ACGT 1 cut(s) 542
HpyCH4V TGCA 9 cut(s) 653, 694, 833, 890, 1115, 1189, 1330, 1349, 1673
HpyF10VI GCNNNNNNNGC 2 cut(s) 608, 1770
HpyF3I CTNAG 7 cut(s) 447, 459, 759, 798, 1035, 1248, 1521
HpySE526I ACGT 1 cut(s) 542
Hsp92I GRCGYC 1 cut(s) 1048
Hsp92II CATG 6 cut(s) 968, 1193, 1334, 1381, 1516, 1815
HspAI GCGC 1 cut(s) 345
KspAI GTTAAC 1 cut(s) 670
Kzo9I GATC 6 cut(s) 418, 422, 442, 1103, 1215, 1649
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 1338, 1400, 1453
Lsp1109I GCAGC 3 cut(s) 665, 1298, 1333
LweI GCATC 4 cut(s) 289, 464, 589, 1358
MaeI CTAG 1 cut(s) 1536
MaeII ACGT 1 cut(s) 542
MaeIII GTNAC 3 cut(s) 536, 631, 1084
MalI GATC 6 cut(s) 420, 424, 444, 1105, 1217, 1651
MbiI CCGCTC 2 cut(s) 1563, 1662
MboI GATC 6 cut(s) 418, 422, 442, 1103, 1215, 1649
MflI RGATCY 1 cut(s) 1215
MhlI GDGCHC 1 cut(s) 1527
MlyI GAGTC 6 cut(s) 41, 809, 913, 979, 1046, 1086
Mph1103I ATGCAT 1 cut(s) 1191
MroXI GAANNNNTTC 4 cut(s) 477, 790, 1160, 1774
MseI TTAA 5 cut(s) 492, 669, 780, 996, 1068
MslI CAYNNNNRTG 4 cut(s) 354, 594, 680, 1172
MspA1I CMGCKG 1 cut(s) 1316
MspI CCGG 2 cut(s) 167, 776
MspR9I CCNGG 2 cut(s) 168, 1392
Mva1269I GAATGC 3 cut(s) 613, 904, 1633
MvaI CCWGG 1 cut(s) 1392
MwoI GCNNNNNNNGC 2 cut(s) 608, 1770
NciI CCSGG 1 cut(s) 168
NdeII GATC 6 cut(s) 418, 422, 442, 1103, 1215, 1649
NlaIII CATG 6 cut(s) 968, 1193, 1334, 1381, 1516, 1815
NlaIV GGNNCC 1 cut(s) 64
NmuCI GTSAC 1 cut(s) 536
NsiI ATGCAT 1 cut(s) 1191
NspI RCATGY 2 cut(s) 1193, 1381
NspV TTCGAA 1 cut(s) 198
OliI CACNNNNGTG 1 cut(s) 680
PaeI GCATGC 1 cut(s) 1193
PagI TCATGA 3 cut(s) 964, 1512, 1811
PceI AGGCCT 1 cut(s) 1493
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 3 cut(s) 613, 904, 1633
PdmI GAANNNNTTC 4 cut(s) 477, 790, 1160, 1774
PfeI GAWTC 5 cut(s) 586, 617, 728, 935, 1463
PflMI CCANNNNNTGG 1 cut(s) 1397
PkrI GCNGC 4 cut(s) 655, 1288, 1348, 1565
PleI GAGTC 6 cut(s) 41, 808, 913, 978, 1045, 1085
PpsI GAGTC 6 cut(s) 41, 808, 913, 978, 1045, 1085
Psp6I CCWGG 1 cut(s) 1390
PspGI CCWGG 1 cut(s) 1390
PspN4I GGNNCC 1 cut(s) 64
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 1 cut(s) 1215
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 3 cut(s) 380, 850, 1371
RsaNI GTAC 3 cut(s) 379, 849, 1370
RseI CAYNNNNRTG 4 cut(s) 354, 594, 680, 1172
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 5 cut(s) 492, 669, 780, 996, 1068
SatI GCNGC 4 cut(s) 654, 1287, 1347, 1564
Sau3AI GATC 6 cut(s) 418, 422, 442, 1103, 1215, 1649
SchI GAGTC 6 cut(s) 41, 809, 913, 979, 1046, 1086
ScrFI CCNGG 2 cut(s) 168, 1392
SduI GDGCHC 1 cut(s) 1527
SfaNI GCATC 4 cut(s) 289, 464, 589, 1358
SfcI CTRYAG 2 cut(s) 250, 651
SfuI TTCGAA 1 cut(s) 198
SmiMI CAYNNNNRTG 4 cut(s) 354, 594, 680, 1172
SmlI CTYRAG 3 cut(s) 31, 1408, 1495
SmoI CTYRAG 3 cut(s) 31, 1408, 1495
SphI GCATGC 1 cut(s) 1193
SseBI AGGCCT 1 cut(s) 1493
SsiI CCGC 4 cut(s) 255, 1264, 1563, 1662
SspMI CTAG 1 cut(s) 1536
StuI AGGCCT 1 cut(s) 1493
StyD4I CCNGG 2 cut(s) 166, 1390
StyI CCWWGG 2 cut(s) 1003, 1194
TaaI ACNGT 1 cut(s) 1798
TaiI ACGT 1 cut(s) 545
TaqI TCGA 5 cut(s) 198, 243, 421, 973, 1648
TaqII GACCGA 1 cut(s) 587
TatI WGTACW 1 cut(s) 1369
TauI GCSGC 1 cut(s) 1566
TfiI GAWTC 5 cut(s) 586, 617, 728, 935, 1463
Tru1I TTAA 5 cut(s) 492, 669, 780, 996, 1068
Tru9I TTAA 5 cut(s) 492, 669, 780, 996, 1068
TscAI CASTG 1 cut(s) 1332
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 3 cut(s) 653, 1286, 1346
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 9 cut(s) 105, 345, 423, 486, 576, 630, 653, 1223, 1501
TspGWI ACGGA 3 cut(s) 339, 399, 875
TspRI CASTG 1 cut(s) 1332
Van91I CCANNNNNTGG 1 cut(s) 1397
XapI RAATTY 4 cut(s) 235, 314, 735, 1321
XceI RCATGY 2 cut(s) 1193, 1381
XmnI GAANNNNTTC 4 cut(s) 477, 790, 1160, 1774
XspI CTAG 1 cut(s) 1536
Zsp2I ATGCAT 1 cut(s) 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.