pycom13g05060

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008523
Physical Location & Seq
Reverse (-)
3388223 .. 3390949
2727 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGGTGACGCTGATCGTGGCGACGACTACCGACCCGGCCTCCGTCAACCCGGCCAATGCCCTCCTAGCCATGCCCGGCTGGGACCCCTCCCCTTCCTGTTTACAGGACATGAAGGGGTTTGTCAACGGAGGAGGAGGAGGAGTCAGGGTTTTGCTGCACGGTAGGGGAATTGTGGAAGAAGACGACTTGGACAAGCGGTGGGAGGAGGCCACCGGCGAGCGAGTCGACGAGGTGATATTTCTGAGCAAACACACTGCCGTCTCCAACCGGCCGGCACTGACCGTGCACCCGATCGGGATCCCTCATCTGCGTGAAGGCGAGGCTGGACCGCAAGGCGGAAAGCCCGGTTGGGCTGCGCCGCCGAGCCCCCGCATTGGTCCCTGGCTCCGGCACCTGAAAAAGCTTGCCCAAGCCCACAACTTGGTTCCTGAGTTCGAGATTACTTTGGAGGGTACACATCATGGACCGGTCACGAATACACTTACTATGTTCTTGGAGATTGGCAGCACGGATGAGTACTGGAAGAGGCAGGATGCAGCACAAGTTATGGCTCTATTAGTTTGGGAAGGACTTGGGCTTGGAGGGGAAGCTGCTGTTGGAAACTGGAACGGGGAGAAGGATAAGAATAAAGTTCTTCTTGGATTAGGCGGTGGACATTATGCTCCTCGGCATATGGATATAGTTCTGAAAGACGGTGTGTGGGTAGGCCATCTACTTTCGGGTTATTCCTTGCCGATGGAAGATCCAAACCAGTCCAAAGCAGAAACAAATGCAAAAGATACGAAGGACATTGGTGGAACTTGGAAACAATCGATCAAAGCAGCATTTGAGGCTACCCAGTCTGCTTTCCCTGGTGGGGAAGTTATAGCTCATCTTGATCAGAAGAGTTTCAAAAGCTGGCAAAAGAATGCTATAACGGCTTTCCTAGGCGAGCAGAACATCAAAATTGGCAAACCCAACGATTTCTACTAA

Protein Analysis

324

Amino Acids

34.92

Weight (kDa)

5.95

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 72 - 316 1.8e-56 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 390
AccB7I CCANNNNNTGG 1 cut(s) 421
AccI GTMKAC 1 cut(s) 225
AciI CCGC 6 cut(s) 196, 329, 336, 359, 370, 648
AclWI GGATC 3 cut(s) 292, 305, 737
AcoI YGGCCR 2 cut(s) 51, 269
AfaI GTAC 2 cut(s) 454, 518
AfiI CCNNNNNNNGG 5 cut(s) 335, 374, 387, 421, 856
AgeI ACCGGT 1 cut(s) 466
AgsI TTSAA 1 cut(s) 892
AjnI CCWGG 2 cut(s) 380, 850
AjuI GAANNNNNNNTTGG 4 cut(s) 331, 363, 579, 611
AluBI AGCT 4 cut(s) 403, 590, 869, 897
AluI AGCT 4 cut(s) 403, 590, 869, 897
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 1 cut(s) 265
Alw44I GTGCAC 1 cut(s) 284
AlwI GGATC 3 cut(s) 292, 305, 737
AoxI GGCC 5 cut(s) 36, 51, 207, 269, 706
ApaLI GTGCAC 1 cut(s) 284
ApeKI GCWGC 6 cut(s) 154, 353, 504, 536, 590, 821
AsiGI ACCGGT 1 cut(s) 466
Asp700I GAANNNNTTC 1 cut(s) 887
AspA2I CCTAGG 1 cut(s) 925
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 4 cut(s) 82, 326, 377, 464
AsuC2I CCSGG 4 cut(s) 35, 50, 75, 345
AsuHPI GGTGA 2 cut(s) 16, 244
AvaII GGWCC 4 cut(s) 82, 326, 377, 464
AvrII CCTAGG 1 cut(s) 925
BaeGI GKGCMC 1 cut(s) 288
BamHI GGATCC 1 cut(s) 297
BanI GGYRCC 1 cut(s) 390
BanII GRGCYC 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 6 cut(s) 141, 340, 516, 548, 577, 833
BccI CCATC 2 cut(s) 717, 730
BceAI ACGGC 2 cut(s) 242, 933
BciT130I CCWGG 2 cut(s) 382, 852
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 4 cut(s) 35, 50, 75, 345
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 2 cut(s) 65, 926
BisI GCNGC 7 cut(s) 155, 354, 359, 505, 537, 591, 822
BlnI CCTAGG 1 cut(s) 925
BlsI GCNGC 7 cut(s) 156, 355, 360, 506, 538, 592, 823
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 6 cut(s) 35, 50, 75, 345, 382, 852
Bme18I GGWCC 4 cut(s) 82, 326, 377, 464
BmgT120I GGNCC 4 cut(s) 82, 326, 377, 464
BmiI GGNNCC 7 cut(s) 83, 84, 299, 379, 386, 392, 426
BmrFI CCNGG 6 cut(s) 35, 50, 75, 345, 382, 852
BmrI ACTGGG 1 cut(s) 832
BmsI GCATC 1 cut(s) 523
BmuI ACTGGG 1 cut(s) 832
BpiI GAAGAC 1 cut(s) 186
BpuMI CCSGG 4 cut(s) 35, 50, 75, 345
Bsa29I ATCGAT 1 cut(s) 812
BsaBI GATNNNNATC 1 cut(s) 296
BsaJI CCNNGG 4 cut(s) 380, 665, 850, 925
BsaWI WCCGGW 1 cut(s) 466
BsaXI ACNNNNNCTCC 6 cut(s) 23, 53, 126, 132, 156, 162
Bsc4I CCNNNNNNNGG 5 cut(s) 335, 374, 387, 421, 856
Bse118I RCCGGY 4 cut(s) 212, 267, 271, 466
Bse1I ACTGG 4 cut(s) 524, 608, 751, 838
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 2 cut(s) 382, 852
BseCI ATCGAT 1 cut(s) 812
BseDI CCNNGG 4 cut(s) 380, 665, 850, 925
BseGI GGATG 2 cut(s) 517, 538
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 5 cut(s) 335, 374, 387, 421, 856
BseMII CTCAG 2 cut(s) 233, 420
BseNI ACTGG 4 cut(s) 524, 608, 751, 838
BseRI GAGGAG 6 cut(s) 144, 147, 150, 153, 218, 654
BseSI GKGCMC 1 cut(s) 288
BseX3I CGGCCG 1 cut(s) 269
BseXI GCAGC 6 cut(s) 141, 340, 516, 548, 577, 833
BseYI CCCAGC 1 cut(s) 78
BsgI GTGCAG 1 cut(s) 140
Bsh1285I CGRYCG 2 cut(s) 272, 294
BshFI GGCC 5 cut(s) 38, 53, 209, 271, 708
BshNI GGYRCC 1 cut(s) 390
BshTI ACCGGT 1 cut(s) 466
BshVI ATCGAT 1 cut(s) 812
BsiEI CGRYCG 2 cut(s) 272, 294
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 9 cut(s) 35, 50, 75, 213, 268, 272, 345, 388, 467
BslFI GGGAC 2 cut(s) 95, 363
BslI CCNNNNNNNGG 5 cut(s) 335, 374, 387, 421, 856
BsmAI GTCTC 1 cut(s) 265
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 2 cut(s) 95, 363
BsmI GAATGC 1 cut(s) 913
BsnI GGCC 5 cut(s) 38, 53, 209, 271, 708
Bsp1286I GDGCHC 2 cut(s) 288, 368
Bsp143I GATC 6 cut(s) 12, 291, 297, 742, 813, 877
BspACI CCGC 6 cut(s) 196, 329, 336, 359, 370, 648
BspANI GGCC 5 cut(s) 38, 53, 209, 271, 708
BspCNI CTCAG 2 cut(s) 234, 421
BspDI ATCGAT 1 cut(s) 812
BspLI GGNNCC 7 cut(s) 83, 84, 299, 379, 386, 392, 426
BspPI GGATC 3 cut(s) 292, 305, 737
BspT107I GGYRCC 1 cut(s) 390
BsrFI RCCGGY 4 cut(s) 212, 267, 271, 466
BsrI ACTGG 4 cut(s) 524, 608, 751, 838
BssAI RCCGGY 4 cut(s) 212, 267, 271, 466
BssECI CCNNGG 4 cut(s) 380, 665, 850, 925
BssMI GATC 6 cut(s) 12, 291, 297, 742, 813, 877
BssT1I CCWWGG 1 cut(s) 925
Bst2UI CCWGG 2 cut(s) 382, 852
Bst4CI ACNGT 3 cut(s) 161, 283, 695
Bst6I CTCTTC 2 cut(s) 518, 878
BstC8I GCNNGC 5 cut(s) 218, 273, 405, 899, 932
BstDEI CTNAG 2 cut(s) 242, 429
BstF5I GGATG 2 cut(s) 517, 538
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 6 cut(s) 15, 294, 300, 745, 816, 880
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 6 cut(s) 12, 291, 297, 742, 813, 877
BstMCI CGRYCG 2 cut(s) 272, 294
BstMWI GCNNNNNNNGC 3 cut(s) 65, 830, 917
BstNI CCWGG 2 cut(s) 382, 852
BstSCI CCNGG 6 cut(s) 33, 48, 73, 343, 380, 850
BstSLI GKGCMC 1 cut(s) 288
BstV1I GCAGC 6 cut(s) 141, 340, 516, 548, 577, 833
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 2 cut(s) 297, 742
BstYI RGATCY 2 cut(s) 297, 742
BstZI CGGCCG 1 cut(s) 269
Bsu15I ATCGAT 1 cut(s) 812
BsuRI GGCC 5 cut(s) 38, 53, 209, 271, 708
BsuTUI ATCGAT 1 cut(s) 812
BtsCI GGATG 2 cut(s) 517, 538
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 252, 275
Cac8I GCNNGC 5 cut(s) 218, 273, 405, 899, 932
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 4 cut(s) 212, 267, 271, 466
Cfr13I GGNCC 4 cut(s) 82, 326, 377, 464
ClaI ATCGAT 1 cut(s) 812
CseI GACGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 453, 517
CspAI ACCGGT 1 cut(s) 466
CviAII CATG 3 cut(s) 70, 109, 461
CviQI GTAC 2 cut(s) 453, 517
DdeI CTNAG 2 cut(s) 242, 429
DpnI GATC 6 cut(s) 14, 293, 299, 744, 815, 879
DpnII GATC 6 cut(s) 12, 291, 297, 742, 813, 877
EaeI YGGCCR 2 cut(s) 51, 269
EagI CGGCCG 1 cut(s) 269
Eam1104I CTCTTC 2 cut(s) 518, 878
EarI CTCTTC 2 cut(s) 518, 878
EciI GGCGGA 1 cut(s) 351
EclXI CGGCCG 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 925
Eco24I GRGCYC 1 cut(s) 368
Eco47I GGWCC 4 cut(s) 82, 326, 377, 464
Eco52I CGGCCG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 380, 850
EcoT14I CCWWGG 1 cut(s) 925
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 925
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 3 cut(s) 73, 112, 464
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FaqI GGGAC 2 cut(s) 95, 363
FatI CATG 3 cut(s) 69, 108, 460
FauI CCCGC 1 cut(s) 377
FauNDI CATATG 1 cut(s) 672
FbaI TGATCA 1 cut(s) 877
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 7 cut(s) 155, 354, 359, 505, 537, 591, 822
FokI GGATG 2 cut(s) 524, 545
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 7 cut(s) 155, 354, 359, 505, 537, 591, 822
FspBI CTAG 2 cut(s) 65, 926
GlaI GCGC 1 cut(s) 357
GluI GCNGC 7 cut(s) 155, 354, 359, 505, 537, 591, 822
GsaI CCCAGC 1 cut(s) 82
HaeIII GGCC 5 cut(s) 38, 53, 209, 271, 708
HapII CCGG 9 cut(s) 35, 50, 75, 213, 268, 272, 345, 388, 467
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 358
Hin1II CATG 3 cut(s) 73, 112, 464
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HincII GTYRAC 3 cut(s) 46, 124, 226
HindII GTYRAC 3 cut(s) 46, 124, 226
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 2 cut(s) 141, 222
HpaII CCGG 9 cut(s) 35, 50, 75, 213, 268, 272, 345, 388, 467
HphI GGTGA 2 cut(s) 16, 244
Hpy166II GTNNAC 7 cut(s) 46, 101, 124, 226, 286, 455, 653
Hpy188I TCNGA 3 cut(s) 243, 687, 882
Hpy188III TCNNGA 5 cut(s) 295, 428, 436, 472, 875
Hpy8I GTNNAC 7 cut(s) 46, 101, 124, 226, 286, 455, 653
Hpy99I CGWCG 2 cut(s) 25, 230
HpyAV CCTTC 6 cut(s) 102, 106, 308, 560, 610, 778
HpyCH4III ACNGT 3 cut(s) 161, 283, 695
HpyCH4V TGCA 4 cut(s) 157, 286, 536, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 830, 917
HpyF3I CTNAG 2 cut(s) 242, 429
Hsp92II CATG 3 cut(s) 73, 112, 464
HspAI GCGC 1 cut(s) 356
KflI GGGWCCC 1 cut(s) 82
KroI GCCGGC 1 cut(s) 271
KroNI GCCGGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 6 cut(s) 12, 291, 297, 742, 813, 877
LmnI GCTCC 2 cut(s) 390, 667
Lsp1109I GCAGC 6 cut(s) 141, 340, 516, 548, 577, 833
LweI GCATC 1 cut(s) 523
MaeI CTAG 2 cut(s) 65, 926
MaeIII GTNAC 2 cut(s) 4, 469
MalI GATC 6 cut(s) 14, 293, 299, 744, 815, 879
MboI GATC 6 cut(s) 12, 291, 297, 742, 813, 877
MboII GAAGA 6 cut(s) 188, 191, 535, 626, 752, 895
MflI RGATCY 2 cut(s) 297, 742
MhlI GDGCHC 2 cut(s) 288, 368
MluCI AATT 2 cut(s) 168, 945
MlyI GAGTC 2 cut(s) 150, 231
MmeI TCCRAC 2 cut(s) 288, 577
MroNI GCCGGC 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 887
MslI CAYNNNNRTG 1 cut(s) 309
MspI CCGG 9 cut(s) 35, 50, 75, 213, 268, 272, 345, 388, 467
MspR9I CCNGG 6 cut(s) 35, 50, 75, 345, 382, 852
Mva1269I GAATGC 1 cut(s) 913
MvaI CCWGG 2 cut(s) 382, 852
MwoI GCNNNNNNNGC 3 cut(s) 65, 830, 917
NaeI GCCGGC 1 cut(s) 273
NciI CCSGG 4 cut(s) 35, 50, 75, 345
NdeI CATATG 1 cut(s) 672
NdeII GATC 6 cut(s) 12, 291, 297, 742, 813, 877
NgoMIV GCCGGC 1 cut(s) 271
NlaIII CATG 3 cut(s) 73, 112, 464
NlaIV GGNNCC 7 cut(s) 83, 84, 299, 379, 386, 392, 426
NmeAIII GCCGAG 2 cut(s) 387, 646
NmuCI GTSAC 2 cut(s) 4, 469
PctI GAATGC 1 cut(s) 913
PdiI GCCGGC 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 887
PflMI CCANNNNNTGG 1 cut(s) 421
PinAI ACCGGT 1 cut(s) 466
PkrI GCNGC 7 cut(s) 156, 355, 360, 506, 538, 592, 823
Ple19I CGATCG 1 cut(s) 294
PleI GAGTC 2 cut(s) 149, 230
PpsI GAGTC 2 cut(s) 149, 230
PpuMI RGGWCCY 1 cut(s) 82
Psp5II RGGWCCY 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 380, 850
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 2 cut(s) 380, 850
PspN4I GGNNCC 7 cut(s) 83, 84, 299, 379, 386, 392, 426
PspPI GGNCC 4 cut(s) 82, 326, 377, 464
PspPPI RGGWCCY 1 cut(s) 82
PsuI RGATCY 2 cut(s) 297, 742
PvuI CGATCG 1 cut(s) 294
RsaI GTAC 2 cut(s) 454, 518
RsaNI GTAC 2 cut(s) 453, 517
RseI CAYNNNNRTG 1 cut(s) 309
SalI GTCGAC 1 cut(s) 224
SatI GCNGC 7 cut(s) 155, 354, 359, 505, 537, 591, 822
Sau3AI GATC 6 cut(s) 12, 291, 297, 742, 813, 877
Sau96I GGNCC 4 cut(s) 82, 326, 377, 464
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 2 cut(s) 150, 231
ScrFI CCNGG 6 cut(s) 35, 50, 75, 345, 382, 852
SduI GDGCHC 2 cut(s) 288, 368
SetI ASST 6 cut(s) 234, 396, 405, 592, 871, 899
SfaNI GCATC 1 cut(s) 523
SgrAI CRCCGGYG 1 cut(s) 212
SinI GGWCC 4 cut(s) 82, 326, 377, 464
SmiMI CAYNNNNRTG 1 cut(s) 309
Sse9I AATT 2 cut(s) 168, 945
SsiI CCGC 6 cut(s) 196, 329, 336, 359, 370, 648
SspMI CTAG 2 cut(s) 65, 926
StyD4I CCNGG 6 cut(s) 33, 48, 73, 343, 380, 850
StyI CCWWGG 1 cut(s) 925
TaaI ACNGT 3 cut(s) 161, 283, 695
TaqI TCGA 3 cut(s) 225, 435, 812
TasI AATT 2 cut(s) 168, 945
TatI WGTACW 1 cut(s) 516
TauI GCSGC 1 cut(s) 361
TscAI CASTG 2 cut(s) 259, 282
TseFI GTSAC 2 cut(s) 4, 469
TseI GCWGC 6 cut(s) 154, 353, 504, 536, 590, 821
Tsp45I GTSAC 2 cut(s) 4, 469
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 3 cut(s) 31, 141, 524
TspRI CASTG 2 cut(s) 259, 282
Van91I CCANNNNNTGG 1 cut(s) 421
VneI GTGCAC 1 cut(s) 284
VpaK11BI GGWCC 4 cut(s) 82, 326, 377, 464
XmaJI CCTAGG 1 cut(s) 925
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 887
XspI CTAG 2 cut(s) 65, 926
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.