pycom13g06810

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011725
Physical Location & Seq
Reverse (-)
4504682 .. 4505649
968 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGGCGACCAATCCAATTGACGCCTGCTGGAGGTGTGACGCTAATTGGGCCAATAACCGAAAGAAGATTGTAGGCTGCGCCCAAGGCTTTGGTAAAAAGGCCACCGGAGGTAAGGACGGTCCCATTTACGTGGTGACTGTGGGCACAGATAATGACGTACAAAACCCCAAACCCGGAACCCTACGCCACGCCGTGATTCAGAAAGGGCCTTTGTGGATAATCTTCGCAAGAAGCATGGTGATTAGGCTGGAGCAAGAGCTGATGGTGACCAGTGACAAGACCATCGATGGTAGAGGAGCCAATGTTGTGATAGAAGAGGGTGGTGGCATTACCCTCCAGTTTGTGAAGAATGTAATCATCACCAACCTTCACATCAAAATGATTGTGTCCAAACCCGGTGGTATGATCAGGGACTCCGTCGACCACATGGGTTTGAGGACCAAGAGTGACGGCGATGGTATCTCCCTCTTTGGTGCCTCCAACGTGATGTTGTTTGGCGCCAACAACGCGCACACTCAAGACAAAATCATGCAAATTACTTTGGCCTTCAACCACTTTGGTCAGGGACTGGTGCAGAGGATGCCGAGGGTCCGGCACGGATTCTTCCACGTGGTGAACAACGACTACAACCACTGGATCATGTATGCCATCGGAGGAAACATGAACCCTACCATCATCAGCCAGGGTAACAGGTTCATTGCTCCCGCGAACGCGGCCACCAAACAGGTAACATACAGTTTACATGAATTAATTTTTATTAATTAG

Protein Analysis

255

Amino Acids

27.91

Weight (kDa)

9.54

Isoelectric Point (pI)

23.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 160 - 241 5.2e-09 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 473, 497
AccI GTMKAC 1 cut(s) 420
AccII CGCG 3 cut(s) 509, 707, 713
AciI CCGC 2 cut(s) 705, 713
AclWI GGATC 1 cut(s) 644
AcoI YGGCCR 1 cut(s) 714
AcvI CACGTG 1 cut(s) 610
AcyI GRCGYC 2 cut(s) 21, 498
AdeI CACNNNGTG 2 cut(s) 193, 613
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 2 cut(s) 30, 173
AgsI TTSAA 1 cut(s) 550
AjnI CCWGG 1 cut(s) 681
AluBI AGCT 1 cut(s) 259
AluI AGCT 1 cut(s) 259
AlwI GGATC 1 cut(s) 644
AlwNI CAGNNNCTG 1 cut(s) 568
AoxI GGCC 5 cut(s) 48, 99, 206, 543, 714
ApeKI GCWGC 1 cut(s) 75
AseI ATTAAT 2 cut(s) 749, 759
AspLEI GCGC 3 cut(s) 80, 500, 511
AspS9I GGNCC 5 cut(s) 48, 119, 206, 438, 589
AsuC2I CCSGG 2 cut(s) 174, 396
AsuHPI GGTGA 5 cut(s) 145, 250, 277, 352, 625
AvaII GGWCC 3 cut(s) 119, 438, 589
BaeGI GKGCMC 1 cut(s) 146
BanI GGYRCC 2 cut(s) 473, 497
BbrPI CACGTG 1 cut(s) 610
BbvI GCAGC 1 cut(s) 62
BccI CCATC 6 cut(s) 256, 281, 290, 449, 656, 680
BceAI ACGGC 2 cut(s) 176, 466
BciT130I CCWGG 1 cut(s) 683
BclI TGATCA 1 cut(s) 405
BcnI CCSGG 2 cut(s) 174, 396
BfoI RGCGCY 1 cut(s) 501
BisI GCNGC 2 cut(s) 76, 714
BlsI GCNGC 2 cut(s) 77, 715
Bme1390I CCNGG 3 cut(s) 174, 396, 683
Bme18I GGWCC 3 cut(s) 119, 438, 589
BmgT120I GGNCC 5 cut(s) 48, 119, 206, 438, 589
BmiI GGNNCC 6 cut(s) 121, 178, 298, 475, 499, 590
BmrFI CCNGG 3 cut(s) 174, 396, 683
BmsI GCATC 1 cut(s) 570
BpmI CTGGAG 3 cut(s) 49, 269, 320
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 2 cut(s) 174, 396
Bsa29I ATCGAT 1 cut(s) 285
BsaAI YACGTR 2 cut(s) 130, 610
BsaHI GRCGYC 2 cut(s) 21, 498
BsaJI CCNNGG 3 cut(s) 82, 584, 682
BsaWI WCCGGW 1 cut(s) 104
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 173
Bse1I ACTGG 4 cut(s) 270, 337, 573, 638
Bse3DI GCAATG 1 cut(s) 696
BseBI CCWGG 1 cut(s) 683
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 3 cut(s) 82, 584, 682
BseGI GGATG 1 cut(s) 585
BseLI CCNNNNNNNGG 2 cut(s) 30, 173
BseMI GCAATG 1 cut(s) 696
BseNI ACTGG 4 cut(s) 270, 337, 573, 638
BseRI GAGGAG 1 cut(s) 309
BseSI GKGCMC 1 cut(s) 146
BseXI GCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 593
Bsh1236I CGCG 3 cut(s) 509, 707, 713
BshFI GGCC 5 cut(s) 50, 101, 208, 545, 716
BshNI GGYRCC 2 cut(s) 473, 497
BshVI ATCGAT 1 cut(s) 285
BsiSI CCGG 4 cut(s) 105, 174, 396, 592
BslFI GGGAC 3 cut(s) 105, 425, 579
BslI CCNNNNNNNGG 2 cut(s) 30, 173
BsmFI GGGAC 3 cut(s) 105, 425, 579
BsnI GGCC 5 cut(s) 50, 101, 208, 545, 716
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 2 cut(s) 405, 636
BspACI CCGC 2 cut(s) 705, 713
BspANI GGCC 5 cut(s) 50, 101, 208, 545, 716
BspDI ATCGAT 1 cut(s) 285
BspFNI CGCG 3 cut(s) 509, 707, 713
BspLI GGNNCC 6 cut(s) 121, 178, 298, 475, 499, 590
BspPI GGATC 1 cut(s) 644
BspT107I GGYRCC 2 cut(s) 473, 497
BsrDI GCAATG 1 cut(s) 696
BsrI ACTGG 4 cut(s) 270, 337, 573, 638
BssECI CCNNGG 3 cut(s) 82, 584, 682
BssMI GATC 2 cut(s) 405, 636
BssNI GRCGYC 2 cut(s) 21, 498
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 683
Bst4CI ACNGT 3 cut(s) 119, 139, 737
Bst6I CTCTTC 1 cut(s) 309
BstACI GRCGYC 2 cut(s) 21, 498
BstAPI GCANNNNNTGC 1 cut(s) 580
BstBAI YACGTR 2 cut(s) 130, 610
BstC8I GCNNGC 1 cut(s) 25
BstEII GGTNACC 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 28
BstF5I GGATG 1 cut(s) 585
BstFNI CGCG 3 cut(s) 509, 707, 713
BstH2I RGCGCY 1 cut(s) 501
BstHHI GCGC 3 cut(s) 80, 500, 511
BstKTI GATC 2 cut(s) 408, 639
BstMBI GATC 2 cut(s) 405, 636
BstMWI GCNNNNNNNGC 5 cut(s) 47, 84, 506, 580, 713
BstNI CCWGG 1 cut(s) 683
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 3 cut(s) 172, 394, 681
BstSLI GKGCMC 1 cut(s) 146
BstUI CGCG 3 cut(s) 509, 707, 713
BstV1I GCAGC 1 cut(s) 62
BstXI CCANNNNNNTGG 2 cut(s) 89, 130
Bsu15I ATCGAT 1 cut(s) 285
BsuRI GGCC 5 cut(s) 50, 101, 208, 545, 716
BsuTUI ATCGAT 1 cut(s) 285
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 1 cut(s) 585
BtsIMutI CAGTG 2 cut(s) 277, 631
Cac8I GCNNGC 1 cut(s) 25
CaiI CAGNNNCTG 1 cut(s) 568
CfoI GCGC 3 cut(s) 80, 500, 511
Cfr13I GGNCC 5 cut(s) 48, 119, 206, 438, 589
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 2 cut(s) 29, 47
Csp6I GTAC 1 cut(s) 158
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviAII CATG 6 cut(s) 235, 427, 529, 640, 661, 743
CviQI GTAC 1 cut(s) 158
DinI GGCGCC 1 cut(s) 499
DpnI GATC 2 cut(s) 407, 638
DpnII GATC 2 cut(s) 405, 636
DraIII CACNNNGTG 2 cut(s) 193, 613
EaeI YGGCCR 1 cut(s) 714
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 82
Eco47I GGWCC 3 cut(s) 119, 438, 589
Eco72I CACGTG 1 cut(s) 610
Eco91I GGTNACC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 206
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 681
EcoT14I CCWWGG 1 cut(s) 82
EgeI GGCGCC 1 cut(s) 499
EheI GGCGCC 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 6 cut(s) 238, 430, 532, 643, 664, 746
FaiI YATR 9 cut(s) 236, 404, 428, 530, 641, 645, 662, 733, 744
FaqI GGGAC 3 cut(s) 105, 425, 579
FatI CATG 6 cut(s) 234, 426, 528, 639, 660, 742
FauI CCCGC 1 cut(s) 712
FbaI TGATCA 1 cut(s) 405
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 76, 714
FokI GGATG 1 cut(s) 592
Fsp4HI GCNGC 2 cut(s) 76, 714
GlaI GCGC 3 cut(s) 79, 499, 510
GluI GCNGC 2 cut(s) 76, 714
GsuI CTGGAG 3 cut(s) 49, 269, 320
HaeII RGCGCY 1 cut(s) 501
HaeIII GGCC 5 cut(s) 50, 101, 208, 545, 716
HapII CCGG 4 cut(s) 105, 174, 396, 592
HgaI GACGC 2 cut(s) 29, 47
HhaI GCGC 3 cut(s) 80, 500, 511
Hin1I GRCGYC 2 cut(s) 21, 498
Hin1II CATG 6 cut(s) 238, 430, 532, 643, 664, 746
Hin6I GCGC 3 cut(s) 78, 498, 509
HinP1I GCGC 3 cut(s) 78, 498, 509
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 3 cut(s) 196, 413, 600
HpaII CCGG 4 cut(s) 105, 174, 396, 592
HphI GGTGA 5 cut(s) 145, 250, 277, 352, 625
Hpy166II GTNNAC 3 cut(s) 421, 616, 740
Hpy188I TCNGA 2 cut(s) 201, 653
Hpy188III TCNNGA 1 cut(s) 518
Hpy8I GTNNAC 3 cut(s) 421, 616, 740
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 2 cut(s) 377, 556
HpyCH4III ACNGT 3 cut(s) 119, 139, 737
HpyCH4IV ACGT 4 cut(s) 129, 156, 483, 609
HpyCH4V TGCA 2 cut(s) 532, 574
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 84, 506, 580, 713
HpySE526I ACGT 4 cut(s) 129, 156, 483, 609
Hsp92I GRCGYC 2 cut(s) 21, 498
Hsp92II CATG 6 cut(s) 238, 430, 532, 643, 664, 746
HspAI GCGC 3 cut(s) 78, 498, 509
KasI GGCGCC 1 cut(s) 497
Ksp22I TGATCA 1 cut(s) 405
Kzo9I GATC 2 cut(s) 405, 636
LmnI GCTCC 3 cut(s) 250, 296, 706
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 570
MaeII ACGT 4 cut(s) 129, 156, 483, 609
MaeIII GTNAC 7 cut(s) 35, 133, 265, 272, 446, 686, 727
MalI GATC 2 cut(s) 407, 638
MboI GATC 2 cut(s) 405, 636
MboII GAAGA 5 cut(s) 76, 214, 326, 358, 595
MfeI CAATTG 1 cut(s) 15
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 6 cut(s) 15, 43, 534, 746, 750, 760
Mly113I GGCGCC 1 cut(s) 498
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 504
MseI TTAA 2 cut(s) 749, 759
MslI CAYNNNNRTG 2 cut(s) 128, 377
MspI CCGG 4 cut(s) 105, 174, 396, 592
MspR9I CCNGG 3 cut(s) 174, 396, 683
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 683
MvnI CGCG 3 cut(s) 509, 707, 713
MwoI GCNNNNNNNGC 5 cut(s) 47, 84, 506, 580, 713
NarI GGCGCC 1 cut(s) 498
NciI CCSGG 2 cut(s) 174, 396
NdeII GATC 2 cut(s) 405, 636
NlaIII CATG 6 cut(s) 238, 430, 532, 643, 664, 746
NlaIV GGNNCC 6 cut(s) 121, 178, 298, 475, 499, 590
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 5 cut(s) 35, 133, 265, 272, 446
PfeI GAWTC 2 cut(s) 196, 600
PflFI GACNNNGTC 1 cut(s) 416
PkrI GCNGC 2 cut(s) 77, 715
PleI GAGTC 1 cut(s) 407
PluTI GGCGCC 1 cut(s) 501
PmaCI CACGTG 1 cut(s) 610
PmlI CACGTG 1 cut(s) 610
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 2 cut(s) 130, 610
PshBI ATTAAT 2 cut(s) 749, 759
Psp6I CCWGG 1 cut(s) 681
PspCI CACGTG 1 cut(s) 610
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 681
PspN4I GGNNCC 6 cut(s) 121, 178, 298, 475, 499, 590
PspPI GGNCC 5 cut(s) 48, 119, 206, 438, 589
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
PstNI CAGNNNCTG 1 cut(s) 568
PsyI GACNNNGTC 1 cut(s) 416
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
RseI CAYNNNNRTG 2 cut(s) 128, 377
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 2 cut(s) 749, 759
SatI GCNGC 2 cut(s) 76, 714
Sau3AI GATC 2 cut(s) 405, 636
Sau96I GGNCC 5 cut(s) 48, 119, 206, 438, 589
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 3 cut(s) 174, 396, 683
SduI GDGCHC 1 cut(s) 146
SfaNI GCATC 1 cut(s) 570
SfoI GGCGCC 1 cut(s) 499
SinI GGWCC 3 cut(s) 119, 438, 589
SmiMI CAYNNNNRTG 2 cut(s) 128, 377
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 6 cut(s) 15, 43, 534, 746, 750, 760
SsiI CCGC 2 cut(s) 705, 713
SspDI GGCGCC 1 cut(s) 497
StyD4I CCNGG 3 cut(s) 172, 394, 681
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 3 cut(s) 119, 139, 737
TaiI ACGT 4 cut(s) 132, 159, 486, 612
TaqI TCGA 2 cut(s) 285, 420
TasI AATT 6 cut(s) 15, 43, 534, 746, 750, 760
TauI GCSGC 1 cut(s) 716
TfiI GAWTC 2 cut(s) 196, 600
Tru1I TTAA 2 cut(s) 749, 759
Tru9I TTAA 2 cut(s) 749, 759
TscAI CASTG 2 cut(s) 277, 638
TseFI GTSAC 5 cut(s) 35, 133, 265, 272, 446
TseI GCWGC 1 cut(s) 75
Tsp45I GTSAC 5 cut(s) 35, 133, 265, 272, 446
TspDTI ATGAA 3 cut(s) 677, 685, 759
TspGWI ACGGA 2 cut(s) 406, 612
TspRI CASTG 2 cut(s) 277, 638
Tth111I GACNNNGTC 1 cut(s) 416
VpaK11BI GGWCC 3 cut(s) 119, 438, 589
VspI ATTAAT 2 cut(s) 749, 759
XagI CCTNNNNNAGG 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.