Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

5626748 .. 5636277

Gene Structure 9530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4170 bp

ATGAGCAATTCCGTAGGCCATAACTTGCTCAACCAGAGCCTGCTTCAAAGAAAGGTAAATTCTTCCGGTATTCCTGCCAACACATTGTTTCAAGCTAAGAGCGTCCACCAAGTGACGGCACTGGCGCGAAAATCACCGATATCCAAGAAGTTTTGTGTGAACATTTTGAACGTTCAGAAACCAAAGTTAACCATGGGGTCACGCCGCCCCGTAGCAGCTGTTCCTCGTGCCGTATTAGCCACTAATCCGCCTTCGGATCAGCTTGCTGGGAAATTCAATCTCGATGGGAATACTGAATTGCAGGTTTATGTGAATGCTTCCACTCCGGGATCTCCCACTCAAGTACATTTTCGTGTAGCATATAGCGGTGACTCTTTGACTCTTCACTGGGGTGGGGTACAAGATAGGAAAGAAAAGTGGGTGCTTCCGTCTCGTCACCCAGATGGAACCAAAGTATATAAGAACAGAGCTCTTAGAACACCTTTTGTGAGTTCTGGCTCCAGTTCCTCGCTTCAAATTGAGATTGATGATCCTACAATACAAGCAATCGAGTTTCTTTTAGTTGATGAAAGCCAGAACAGATGGTTTAAGAACAATGGTGATAACTTTCATGTTAAATTACCTGCGAAAGAGAAGCTGATATCCAATGCTCCAGTTCCTGAAGAGCTTGTACAAATTCAAGCATATTTGAGGTGGGAAAGAAAGGGTAAACAGATGTATACACCAGAGCAAGAAAAGGTAGAGTACCAAGCAGCCCGATCTGAGTTATTGGAGGAAGTAGCTAGGGGTAAATCCATACAAGAGCTCCAAGCAAAGTTGACTAAGAAAGATGATGATGATAAAATTGAAGAGCCATCTCATTCTGATACAAAGACTGCAAAGAAAGATGAGAGAAAACAGGTTTTTAGAACCAATAGAATTCAACGCAAGAGGAGGGACTTAATGCAGATGATCAACAAACATGCTTCTAAACCTGTTTACGGGGGTAAAATTGTAGATGAAGAACATCCAGCCAAACCAAAACCCTTGACAGCAGTTGAGCTTTTCGCCAAGGAAAAGGAAGAACAGGATGGAGGTTCTGTTATGAGGAAAAACATTTTCAAGCTCAATGATAAGGAACTTCTGATACTCGTAACCAATCCTGCTGGGAAAACAAGGGTTCATCTGGCAACAGATTTCAAAGAGCCACTTACCCTTCACTGGGCTTTATCTGCAAGCAAGGCTGGAGAGTGGTTGGAACCACCTGCAAATACACTTCCCCAGGGTTCGGTTGCTCTTAATGGGGTTGTTGAAACACAATTTAGACCAGGTTCCGCTGACTCTGCTTATGAGGTTCAATCTTTAGAAATAGAAGTTGAAGCAGATAGTTTTAAAGGAATGCCATTCGTCCTTAATGCTTCTGGGACATGGATGAAGAATCAAGGCTCCAATTTCTATGTTGACTTTGGTGTTGAACGCAAGAAAGCTCAGAAGGATACTGGTGATGGCAAAGGTACTGCAAAGGCTTTGTTGGATAAAATAGCAGGCCAGGAGAGTGAGGCACAGAAATCTTTTATGCATCGATTTAACATTGCAGCAGACTTGGTCAATCAAGCAAAGGATGCTGGTGAATTAGGTCTTGCGGGTATTTTGGTGTGGATGAGGTTTATGGCCACAAGGCAGCTCGTTTGGAATAAAAATTACAATGTGAAACCACGTGAGATTAGTAAAGCTCAGAATAGGCTCACGGACTTGCTTCAGAATGTTTATGTAAATTATCCTCAGTATCGAGAACTTCTACGGATGATATTGTCTACTGTTGGCCGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTCGTCCAGAGAAACAATGATTGCAATGGCTCATTTATGGAGGAATGGCATCAAAAGTTGCATAATAACACGAGTCCTGATGATGTTGTTATTTGTCAGGCATTAATGGATTACATAAAAAATGACTTTGATATTGGTGTTTACTGGAAAACTTTGAATGAGAATGGAATCACAAAAGAACGTCTTCTAAGCTACGATCGTGCAATCCATAATGAACCACAATTCAGAAGAGATCAGAAAGAGGGTCTTCTGCGGGATCTGGGGCATTACATGAGAACTTTGAAGGCTGTTCATTCAGGTGCAGATCTTGAGTCTGCCATTCAAAATTGTATGGGCTACAAAGATGAGGGTCAAGGATTCATGGTTGGAGTAAAAATAAATCCTATATCTGGCTTGCCATCAGAAGTTCCAGATCTCCTGCGATTTGTACTAGAACATGTTGAAGACAGGAATGTGGAAGTCCTTATTGAGGGTTTGCTCGAGGCGCGCCAGGGGCTTAGGACATTGCTCTCTAAGCCTCATGATCGTCTGAGGGATCTTTTATTTTTGGACATTGCCCTTGATTCTACTGTTAGGACCGCTATTGAGAGGGGATATGAAGAACTGAATAATGCTGGACCAGAGAAAATTATGTATTTCATCTCTTTGGTTCTGGAAAACCTGGCACTGTCATCAGATGATAACGAGGATCTCATTTACTGTTTGAAGGGATGGGATCAGGCTCAGAACATGTTAAAAAGTAACAATAATGACTGGGCATTATATTCAAAATCAATCCTAGACAGAACCCGCCTTGCGCTTGCATACAAGGCAGAATCATATCTCAGTATTTTACAGCCGTCTGCAGAATACCTTGGAGCACAGCTTGGAGTGGACCAATGGGCTGTGAACATATTTACCGAAGAAATAATCCGTGCTGGATCAGCTGCCTCTTTATCCTCACTCCTTAATCGGCTTGATCCGGTTCTTCGAAAGACTGCTCATATGGGAAGTTGGCAGGTGATCAGCCCAATTGAAGTGGTTGGATATGTGGTCTATGTGGATGAATTACTCACTGTTCAGAATAAAGTTTACAGAAAGCCCACAATTTTGGTGGCAAAAAGTGTAAAGGGTGAGGAAGAAATTCCAGATGGTACTGTTGCAGTGCTGACACCTGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTGTGTTTTGCGACATGCTTTGACCCCAATATTCTGGCTGACCTCAAGGCTAGTGAAGGGAAGTTATTACGTCTAAAGCCAACACCTGCTGATATAGTTTATAGTGAGGTCAATGCGGGTGAGCTAGATGCAAGTTCAACTGATTCAAGAGAGGACGCTCCATCTCTAACATTGGTCAAGAAAGAATTTACTGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTTGGAGCTAAATCACGTAATATTGCTTATGTAAAAGGAAAACTTCCGTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGATCATTCGAAAAGGTTCTTTCAGAAGATTCGAATAAGGCTGTTGCTAAAAAGTTGGAAACTCTAAAGAAAAAGTTAAAGGACGGAAATTTTGATTTGCTCAAGGAGATTCGGGAAACAGTTTTACAGCTGGCAGCACCACCCCAGTTGGTGCAAGAGCTGAAGACTACGATGAAAGGTTCAGGAATGCCTTGGCCTGGTGATGAAGGCGAACAACGGTGGGAACAAGCATGGACAGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAACGAGAGAGCATACTTCAGCACGAGAAAAGTGAAACTAGATCACGAGTATCTCTGCATGGCTGTCCTTGTTCAGGAAATCATAAATGCTGACTATGCATTCGTTATTCATACAACTAACCCATCTTCAGGAGACTCATCAGAGATATATGCTGAGGTTGTTAAGGGACTTGGAGAGACTCTAGTTGGAGCTTATCCTGGACGTGCTCTAAGTTTTATTAGCAAAAAGAATGATCTGGACTCTCCTCAGGTGTTGGGTTACCCTAGCAAACCCATTGGCCTCTTTATAAGACGATCCATAATCTTTCGATCTGATTCCAATGGTGAAGATCTCGAAGGTTATGCTGGTGCAGGCCTGTATGACAGTGTCCCTATGGACGAGGAAGAGAAAGTCGTGGTCGACTACTCATCTGACCCGCTTATGGTTGACGGAAACTTTCGCAAGACAATCCTCTCTAGCATTGCTCGTGCAGGAAGTGCAATCGAGGAACTGTATGGATCGATCAATAAGACATTGAAGGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1390

Amino Acids

155.23

Weight (kDa)

6.7

Isoelectric Point (pI)

38.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	89 - 207	5.2e-41	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	221 - 276	5.6e-26	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	366 - 482	4.5e-26	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	948 - 1060	1.8e-52	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1201 - 1278	2.6e-13	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3932
AarI	CACCTGC	3 cut(s)	1252, 2839, 3151
AatII	GACGTC	1 cut(s)	3021
Acc36I	ACCTGC	5 cut(s)	292, 631, 1252, 2839, 3151
AccI	GTMKAC	3 cut(s)	719, 1793, 4044
AccII	CGCG	2 cut(s)	127, 2338
AclI	AACGTT	1 cut(s)	171
AcoI	YGGCCR	2 cut(s)	1650, 1801
AcsI	RAATTY	9 cut(s)	58, 272, 675, 918, 1833, 2973, 3242, 3351, 3466
AcuI	CTGAAG	5 cut(s)	681, 1721, 3560, 3646, 3756
AcvI	CACGTG	1 cut(s)	1697
AcyI	GRCGYC	1 cut(s)	3018
AdeI	CACNNNGTG	1 cut(s)	112
AfaI	GTAC	7 cut(s)	345, 399, 672, 746, 1495, 2280, 2986
AflIII	ACRYGT	2 cut(s)	2287, 2580
AjiI	CACGTC	1 cut(s)	3848
AjnI	CCWGG	7 cut(s)	1260, 1306, 1527, 2340, 2511, 3574, 3841
AleI	CACNNNNGTG	1 cut(s)	390
Alw21I	GWGCWC	4 cut(s)	472, 807, 2713, 3853
Alw26I	GTCTC	3 cut(s)	435, 3771, 3815
AlwNI	CAGNNNCTG	2 cut(s)	40, 659
Ama87I	CYCGRG	1 cut(s)	2330
AoxI	GGCC	7 cut(s)	16, 1525, 1650, 1801, 3572, 3922, 3997
ApeKI	GCWGC	6 cut(s)	215, 752, 1574, 1660, 2777, 3512
ApoI	RAATTY	9 cut(s)	58, 272, 675, 918, 1833, 2973, 3242, 3351, 3466
ArsI	GACNNNNNNTTYG	6 cut(s)	1818, 1850, 3452, 3484, 3728, 3760
AscI	GGCGCGCC	1 cut(s)	2336
AseI	ATTAAT	1 cut(s)	1955
Asp700I	GAANNNNTTC	3 cut(s)	2034, 2973, 3393
AspLEI	GCGC	4 cut(s)	127, 2338, 2340, 2650
AspS9I	GGNCC	3 cut(s)	2427, 2468, 2725
AsuC2I	CCSGG	1 cut(s)	327
AsuII	TTCGAA	3 cut(s)	2821, 3387, 3410
AvaI	CYCGRG	1 cut(s)	2330
AvaII	GGWCC	3 cut(s)	2427, 2468, 2725
AxyI	CCTNAGG	1 cut(s)	3891
BalI	TGGCCA	1 cut(s)	1652
BanII	GRGCYC	2 cut(s)	472, 807
BarI	GAAGNNNNNNTAC	2 cut(s)	654, 686
BauI	CACGAG	5 cut(s)	225, 1921, 3667, 3689, 4110
BbrPI	CACGTG	1 cut(s)	1697
BbsI	GAAGAC	4 cut(s)	2027, 2090, 2301, 3548
Bbv12I	GWGCWC	4 cut(s)	472, 807, 2713, 3853
BbvCI	CCTCAGC	1 cut(s)	3798
BbvI	GCAGC	6 cut(s)	227, 764, 1586, 1672, 2764, 3524
BceAI	ACGGC	4 cut(s)	132, 215, 1788, 2674
BcgI	CGANNNNNNTGC	2 cut(s)	3968, 4002
BciT130I	CCWGG	7 cut(s)	1262, 1308, 1529, 2342, 2513, 3576, 3843
BciVI	GTATCC	1 cut(s)	1468
BclI	TGATCA	2 cut(s)	951, 2853
BcnI	CCSGG	1 cut(s)	327
BcoDI	GTCTC	3 cut(s)	435, 3771, 3815
BfaI	CTAG	9 cut(s)	783, 2282, 2630, 3108, 3182, 3683, 3827, 3909, 4101
BfmI	CTRYAG	1 cut(s)	2694
BfuAI	ACCTGC	5 cut(s)	292, 631, 1252, 2839, 3151
BfuI	GTATCC	1 cut(s)	1468
BglII	AGATCT	3 cut(s)	2155, 2263, 3973
BisI	GCNGC	7 cut(s)	205, 216, 753, 1575, 1661, 2778, 3513
BlsI	GCNGC	7 cut(s)	206, 217, 754, 1576, 1662, 2779, 3514
Bme1390I	CCNGG	8 cut(s)	327, 1262, 1308, 1529, 2342, 2513, 3576, 3843
Bme18I	GGWCC	3 cut(s)	2427, 2468, 2725
BmeT110I	CYCGRG	1 cut(s)	2330
BmgBI	CACGTC	1 cut(s)	3848
BmgT120I	GGNCC	3 cut(s)	2427, 2468, 2725
BmiI	GGNNCC	5 cut(s)	448, 499, 1239, 1312, 1426
BmrFI	CCNGG	8 cut(s)	327, 1262, 1308, 1529, 2342, 2513, 3576, 3843
BmrI	ACTGGG	4 cut(s)	397, 1210, 2614, 3517
BmsI	GCATC	4 cut(s)	1567, 1591, 1909, 3175
BmuI	ACTGGG	4 cut(s)	397, 1210, 2614, 3517
BpiI	GAAGAC	4 cut(s)	2027, 2090, 2301, 3548
BpmI	CTGGAG	3 cut(s)	484, 636, 1245
Bpu10I	CCTNAGC	2 cut(s)	2348, 3798
Bpu14I	TTCGAA	3 cut(s)	2821, 3387, 3410
BpuEI	CTTGAG	4 cut(s)	324, 2180, 3086, 3464
BpuMI	CCSGG	1 cut(s)	327
Bsa29I	ATCGAT	2 cut(s)	1561, 4145
BsaAI	YACGTR	2 cut(s)	1697, 3308
BsaHI	GRCGYC	1 cut(s)	3018
BsaJI	CCNNGG	8 cut(s)	192, 1050, 1260, 1261, 1804, 2341, 2704, 3569
BsaWI	WCCGGW	2 cut(s)	65, 2812
BsaXI	ACNNNNNCTCC	4 cut(s)	789, 819, 1065, 1095
Bse21I	CCTNAGG	1 cut(s)	3891
Bse3DI	GCAATG	5 cut(s)	1569, 1882, 2354, 2403, 4104
BseBI	CCWGG	7 cut(s)	1262, 1308, 1529, 2342, 2513, 3576, 3843
BseCI	ATCGAT	2 cut(s)	1561, 4145
BseDI	CCNNGG	8 cut(s)	192, 1050, 1260, 1261, 1804, 2341, 2704, 3569
BseGI	GGATG	9 cut(s)	1006, 1075, 1416, 1606, 1644, 1788, 1825, 2567, 2899
BseMI	GCAATG	5 cut(s)	1569, 1882, 2354, 2403, 4104
BseMII	CTCAG	9 cut(s)	753, 1481, 1727, 1775, 2372, 2588, 2689, 3789, 3905
BsePI	GCGCGC	1 cut(s)	2336
BseRI	GAGGAG	3 cut(s)	946, 1824, 3879
BseXI	GCAGC	6 cut(s)	227, 764, 1586, 1672, 2764, 3524
BseYI	CCCAGC	2 cut(s)	266, 1145
BsgI	GTGCAG	3 cut(s)	2172, 4014, 4134
Bsh1236I	CGCG	2 cut(s)	127, 2338
Bsh1285I	CGRYCG	1 cut(s)	2050
BshFI	GGCC	7 cut(s)	18, 1527, 1652, 1803, 3574, 3924, 3999
BshVI	ATCGAT	2 cut(s)	1561, 4145
BsiEI	CGRYCG	1 cut(s)	2050
BsiHKAI	GWGCWC	4 cut(s)	472, 807, 2713, 3853
BsiHKCI	CYCGRG	1 cut(s)	2330
BsiSI	CCGG	3 cut(s)	66, 326, 2813
BslFI	GGGAC	5 cut(s)	950, 1417, 1839, 3825, 3998
BsmAI	GTCTC	3 cut(s)	435, 3771, 3815
BsmBI	CGTCTC	1 cut(s)	435
BsmFI	GGGAC	5 cut(s)	950, 1417, 1839, 3825, 3998
BsmI	GAATGC	4 cut(s)	319, 1383, 3570, 3743
BsnI	GGCC	7 cut(s)	18, 1527, 1652, 1803, 3574, 3924, 3999
BsoBI	CYCGRG	1 cut(s)	2330
Bsp119I	TTCGAA	3 cut(s)	2821, 3387, 3410
Bsp1286I	GDGCHC	4 cut(s)	472, 807, 2713, 3853
Bsp1407I	TGTACA	1 cut(s)	670
Bsp19I	CCATGG	1 cut(s)	192
BspANI	GGCC	7 cut(s)	18, 1527, 1652, 1803, 3574, 3924, 3999
BspCNI	CTCAG	9 cut(s)	754, 1480, 1726, 1774, 2373, 2587, 2688, 3790, 3904
BspDI	ATCGAT	2 cut(s)	1561, 4145
BspFNI	CGCG	2 cut(s)	127, 2338
BspHI	TCATGA	1 cut(s)	2371
BspLI	GGNNCC	5 cut(s)	448, 499, 1239, 1312, 1426
BspMAI	CTGCAG	1 cut(s)	2698
BspMI	ACCTGC	5 cut(s)	292, 631, 1252, 2839, 3151
BspQI	GCTCTTC	2 cut(s)	657, 843
BspT104I	TTCGAA	3 cut(s)	2821, 3387, 3410
BsrDI	GCAATG	5 cut(s)	1569, 1882, 2354, 2403, 4104
BsrGI	TGTACA	1 cut(s)	670
BssECI	CCNNGG	8 cut(s)	192, 1050, 1260, 1261, 1804, 2341, 2704, 3569
BssHII	GCGCGC	1 cut(s)	2336
BssNAI	GTATAC	1 cut(s)	720
BssNI	GRCGYC	1 cut(s)	3018
BssSI	CACGAG	5 cut(s)	225, 1921, 3667, 3689, 4110
BssT1I	CCWWGG	4 cut(s)	192, 1050, 2704, 3569
Bst1107I	GTATAC	1 cut(s)	720
Bst2BI	CACGAG	5 cut(s)	225, 1921, 3667, 3689, 4110
Bst2UI	CCWGG	7 cut(s)	1262, 1308, 1529, 2342, 2513, 3576, 3843
Bst6I	CTCTTC	5 cut(s)	387, 657, 843, 2074, 4023
BstACI	GRCGYC	1 cut(s)	3018
BstAPI	GCANNNNNTGC	2 cut(s)	1601, 4121
BstAUI	TGTACA	1 cut(s)	670
BstBAI	YACGTR	2 cut(s)	1697, 3308
BstBI	TTCGAA	3 cut(s)	2821, 3387, 3410
BstC8I	GCNNGC	9 cut(s)	41, 264, 1216, 1525, 2246, 2338, 2652, 3510, 3997
BstDSI	CCRYGG	2 cut(s)	192, 1804
BstEII	GGTNACC	1 cut(s)	3902
BstENI	CCTNNNNNAGG	2 cut(s)	2318, 3716
BstF5I	GGATG	9 cut(s)	1006, 1075, 1416, 1606, 1644, 1788, 1825, 2567, 2899
BstFNI	CGCG	2 cut(s)	127, 2338
BstHHI	GCGC	4 cut(s)	127, 2338, 2340, 2650
BstMAI	GTCTC	3 cut(s)	435, 3771, 3815
BstMCI	CGRYCG	1 cut(s)	2050
BstNI	CCWGG	7 cut(s)	1262, 1308, 1529, 2342, 2513, 3576, 3843
BstNSI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
BstPI	GGTNACC	1 cut(s)	3902
BstSCI	CCNGG	8 cut(s)	325, 1260, 1306, 1527, 2340, 2511, 3574, 3841
BstSFI	CTRYAG	1 cut(s)	2694
BstUI	CGCG	2 cut(s)	127, 2338
BstV1I	GCAGC	6 cut(s)	227, 764, 1586, 1672, 2764, 3524
BstV2I	GAAGAC	4 cut(s)	2027, 2090, 2301, 3548
BstX2I	RGATCY	8 cut(s)	329, 1845, 2107, 2155, 2263, 2386, 2539, 3973
BstXI	CCANNNNNNTGG	2 cut(s)	2941, 3091
BstYI	RGATCY	8 cut(s)	329, 1845, 2107, 2155, 2263, 2386, 2539, 3973
BstZ17I	GTATAC	1 cut(s)	720
Bsu15I	ATCGAT	2 cut(s)	1561, 4145
Bsu36I	CCTNAGG	1 cut(s)	3891
BsuI	GTATCC	1 cut(s)	1468
BsuRI	GGCC	7 cut(s)	18, 1527, 1652, 1803, 3574, 3924, 3999
BsuTUI	ATCGAT	2 cut(s)	1561, 4145
BtgI	CCRYGG	2 cut(s)	192, 1804
BtrI	CACGTC	1 cut(s)	3848
BtsCI	GGATG	9 cut(s)	1006, 1075, 1416, 1606, 1644, 1788, 1825, 2567, 2899
BtsI	GCAGTG	1 cut(s)	3000
BtsIMutI	CAGTG	7 cut(s)	119, 385, 1198, 2516, 2904, 3000, 4015
BveI	ACCTGC	5 cut(s)	292, 631, 1252, 2839, 3151
Cac8I	GCNNGC	9 cut(s)	41, 264, 1216, 1525, 2246, 2338, 2652, 3510, 3997
CaiI	CAGNNNCTG	2 cut(s)	40, 659
CciI	TCATGA	1 cut(s)	2371
CfoI	GCGC	4 cut(s)	127, 2338, 2340, 2650
Cfr13I	GGNCC	3 cut(s)	2427, 2468, 2725
ClaI	ATCGAT	2 cut(s)	1561, 4145
CseI	GACGC	2 cut(s)	91, 3221
CsiI	ACCWGGT	1 cut(s)	1306
Csp6I	GTAC	7 cut(s)	344, 398, 671, 745, 1494, 2279, 2985
CspCI	CAANNNNNGTGG	6 cut(s)	2850, 2885, 2925, 2960, 3507, 3542
CviQI	GTAC	7 cut(s)	344, 398, 671, 745, 1494, 2279, 2985
DraI	TTTAAA	1 cut(s)	1372
DraIII	CACNNNGTG	1 cut(s)	112
EaeI	YGGCCR	2 cut(s)	1650, 1801
Eam1104I	CTCTTC	5 cut(s)	387, 657, 843, 2074, 4023
EarI	CTCTTC	5 cut(s)	387, 657, 843, 2074, 4023
EciI	GGCGGA	1 cut(s)	237
Ecl136II	GAGCTC	2 cut(s)	470, 805
Eco130I	CCWWGG	4 cut(s)	192, 1050, 2704, 3569
Eco147I	AGGCCT	1 cut(s)	3999
Eco24I	GRGCYC	2 cut(s)	472, 807
Eco32I	GATATC	2 cut(s)	141, 642
Eco47I	GGWCC	3 cut(s)	2427, 2468, 2725
Eco53kI	GAGCTC	2 cut(s)	470, 805
Eco57I	CTGAAG	5 cut(s)	681, 1721, 3560, 3646, 3756
Eco72I	CACGTG	1 cut(s)	1697
Eco81I	CCTNAGG	1 cut(s)	3891
Eco88I	CYCGRG	1 cut(s)	2330
Eco91I	GGTNACC	1 cut(s)	3902
EcoICRI	GAGCTC	2 cut(s)	470, 805
EcoNI	CCTNNNNNAGG	2 cut(s)	2318, 3716
EcoO65I	GGTNACC	1 cut(s)	3902
EcoRI	GAATTC	3 cut(s)	918, 1833, 3351
EcoRII	CCWGG	7 cut(s)	1260, 1306, 1527, 2340, 2511, 3574, 3841
EcoRV	GATATC	2 cut(s)	141, 642
EcoT14I	CCWWGG	4 cut(s)	192, 1050, 2704, 3569
EcoT22I	ATGCAT	2 cut(s)	1560, 3745
EcoT38I	GRGCYC	2 cut(s)	472, 807
ErhI	CCWWGG	4 cut(s)	192, 1050, 2704, 3569
Esp3I	CGTCTC	1 cut(s)	435
FalI	AAGNNNNNCTT	8 cut(s)	1173, 1205, 2019, 2051, 2082, 2114, 3318, 3350
FaqI	GGGAC	5 cut(s)	950, 1417, 1839, 3825, 3998
FauI	CCCGC	5 cut(s)	1615, 2097, 2648, 3166, 4068
FauNDI	CATATG	1 cut(s)	2835
FbaI	TGATCA	2 cut(s)	951, 2853
FblI	GTMKAC	3 cut(s)	719, 1793, 4044
Fnu4HI	GCNGC	7 cut(s)	205, 216, 753, 1575, 1661, 2778, 3513
FokI	GGATG	9 cut(s)	993, 1082, 1423, 1613, 1651, 1795, 1832, 2574, 2906
FriOI	GRGCYC	2 cut(s)	472, 807
Fsp4HI	GCNGC	7 cut(s)	205, 216, 753, 1575, 1661, 2778, 3513
FspBI	CTAG	9 cut(s)	783, 2282, 2630, 3108, 3182, 3683, 3827, 3909, 4101
GlaI	GCGC	4 cut(s)	126, 2337, 2339, 2649
GluI	GCNGC	7 cut(s)	205, 216, 753, 1575, 1661, 2778, 3513
GsaI	CCCAGC	2 cut(s)	270, 1149
GsuI	CTGGAG	3 cut(s)	484, 636, 1245
HaeIII	GGCC	7 cut(s)	18, 1527, 1652, 1803, 3574, 3924, 3999
HapII	CCGG	3 cut(s)	66, 326, 2813
HgaI	GACGC	2 cut(s)	91, 3221
HhaI	GCGC	4 cut(s)	127, 2338, 2340, 2650
Hin1I	GRCGYC	1 cut(s)	3018
Hin6I	GCGC	4 cut(s)	125, 2336, 2338, 2648
HinP1I	GCGC	4 cut(s)	125, 2336, 2338, 2648
HincII	GTYRAC	5 cut(s)	189, 819, 1441, 4045, 4072
HindII	GTYRAC	5 cut(s)	189, 819, 1441, 4045, 4072
HpaI	GTTAAC	1 cut(s)	189
HpaII	CCGG	3 cut(s)	66, 326, 2813
HpyCH4IV	ACGT	7 cut(s)	171, 1696, 2032, 3018, 3127, 3307, 3847
HpySE526I	ACGT	7 cut(s)	171, 1696, 2032, 3018, 3127, 3307, 3847
Hsp92I	GRCGYC	1 cut(s)	3018
HspAI	GCGC	4 cut(s)	125, 2336, 2338, 2648
Ksp22I	TGATCA	2 cut(s)	951, 2853
KspAI	GTTAAC	1 cut(s)	189
LguI	GCTCTTC	2 cut(s)	657, 843
LmnI	GCTCC	8 cut(s)	503, 655, 810, 1430, 2708, 3220, 3296, 3833
Lsp1109I	GCAGC	6 cut(s)	227, 764, 1586, 1672, 2764, 3524
LweI	GCATC	4 cut(s)	1567, 1591, 1909, 3175
MabI	ACCWGGT	1 cut(s)	1306
MaeI	CTAG	9 cut(s)	783, 2282, 2630, 3108, 3182, 3683, 3827, 3909, 4101
MaeII	ACGT	7 cut(s)	171, 1696, 2032, 3018, 3127, 3307, 3847
MaeIII	GTNAC	7 cut(s)	112, 198, 368, 434, 1132, 2591, 3902
MfeI	CAATTG	1 cut(s)	2862
MflI	RGATCY	8 cut(s)	329, 1845, 2107, 2155, 2263, 2386, 2539, 3973
MhlI	GDGCHC	4 cut(s)	472, 807, 2713, 3853
MlsI	TGGCCA	1 cut(s)	1652
MluNI	TGGCCA	1 cut(s)	1652
MlyI	GAGTC	8 cut(s)	365, 373, 1313, 1933, 2171, 3773, 3817, 3878
MmeI	TCCRAC	8 cut(s)	1215, 1491, 2197, 2854, 3274, 3328, 3414, 3811
Mox20I	TGGCCA	1 cut(s)	1652
Mph1103I	ATGCAT	2 cut(s)	1560, 3745
MroXI	GAANNNNTTC	3 cut(s)	2034, 2973, 3393
MscI	TGGCCA	1 cut(s)	1652
MslI	CAYNNNNRTG	4 cut(s)	351, 390, 441, 2148
Msp20I	TGGCCA	1 cut(s)	1652
MspA1I	CMGCKG	4 cut(s)	218, 1316, 2777, 3508
MspI	CCGG	3 cut(s)	66, 326, 2813
MspR9I	CCNGG	8 cut(s)	327, 1262, 1308, 1529, 2342, 2513, 3576, 3843
MunI	CAATTG	1 cut(s)	2862
Mva1269I	GAATGC	4 cut(s)	319, 1383, 3570, 3743
MvaI	CCWGG	7 cut(s)	1262, 1308, 1529, 2342, 2513, 3576, 3843
MvnI	CGCG	2 cut(s)	127, 2338
NciI	CCSGG	1 cut(s)	327
NcoI	CCATGG	1 cut(s)	192
NdeI	CATATG	1 cut(s)	2835
NlaIV	GGNNCC	5 cut(s)	448, 499, 1239, 1312, 1426
NmuCI	GTSAC	4 cut(s)	112, 198, 368, 434
NsiI	ATGCAT	2 cut(s)	1560, 3745
NspI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
NspV	TTCGAA	3 cut(s)	2821, 3387, 3410
OliI	CACNNNNGTG	1 cut(s)	390
PaeR7I	CTCGAG	1 cut(s)	2330
PagI	TCATGA	1 cut(s)	2371
PalAI	GGCGCGCC	1 cut(s)	2336
PaqCI	CACCTGC	3 cut(s)	1252, 2839, 3151
PasI	CCCWGGG	1 cut(s)	1261
PauI	GCGCGC	1 cut(s)	2336
PceI	AGGCCT	1 cut(s)	3999
PciI	ACATGT	2 cut(s)	2287, 2580
PciSI	GCTCTTC	2 cut(s)	657, 843
PctI	GAATGC	4 cut(s)	319, 1383, 3570, 3743
PdmI	GAANNNNTTC	3 cut(s)	2034, 2973, 3393
PfeI	GAWTC	9 cut(s)	1417, 2019, 2208, 2414, 2666, 3200, 3407, 3487, 3959
PflFI	GACNNNGTC	2 cut(s)	1583, 4010
PfoI	TCCNGGA	2 cut(s)	325, 3841
PkrI	GCNGC	7 cut(s)	206, 217, 754, 1576, 1662, 2779, 3514
Ple19I	CGATCG	1 cut(s)	2050
PleI	GAGTC	8 cut(s)	365, 373, 1313, 1932, 2170, 3773, 3817, 3878
PmaCI	CACGTG	1 cut(s)	1697
PmlI	CACGTG	1 cut(s)	1697
PpsI	GAGTC	8 cut(s)	365, 373, 1313, 1932, 2170, 3773, 3817, 3878
Ppu21I	YACGTR	2 cut(s)	1697, 3308
PscI	ACATGT	2 cut(s)	2287, 2580
PshBI	ATTAAT	1 cut(s)	1955
PsiI	TTATAA	1 cut(s)	3932
Psp124BI	GAGCTC	2 cut(s)	472, 807
Psp1406I	AACGTT	1 cut(s)	171
Psp6I	CCWGG	7 cut(s)	1260, 1306, 1527, 2340, 2511, 3574, 3841
PspCI	CACGTG	1 cut(s)	1697
PspEI	GGTNACC	1 cut(s)	3902
PspFI	CCCAGC	2 cut(s)	266, 1145
PspGI	CCWGG	7 cut(s)	1260, 1306, 1527, 2340, 2511, 3574, 3841
PspN4I	GGNNCC	5 cut(s)	448, 499, 1239, 1312, 1426
PspPI	GGNCC	3 cut(s)	2427, 2468, 2725
PspXI	VCTCGAGB	1 cut(s)	2330
PsrI	GAACNNNNNNTAC	4 cut(s)	204, 236, 1110, 1142
PstI	CTGCAG	1 cut(s)	2698
PstNI	CAGNNNCTG	2 cut(s)	40, 659
PsuI	RGATCY	8 cut(s)	329, 1845, 2107, 2155, 2263, 2386, 2539, 3973
PsyI	GACNNNGTC	2 cut(s)	1583, 4010
PteI	GCGCGC	1 cut(s)	2336
PvuI	CGATCG	1 cut(s)	2050
PvuII	CAGCTG	3 cut(s)	218, 2777, 3508
RsaI	GTAC	7 cut(s)	345, 399, 672, 746, 1495, 2280, 2986
RsaNI	GTAC	7 cut(s)	344, 398, 671, 745, 1494, 2279, 2985
RseI	CAYNNNNRTG	4 cut(s)	351, 390, 441, 2148
SacI	GAGCTC	2 cut(s)	472, 807
SalI	GTCGAC	1 cut(s)	4043
SapI	GCTCTTC	2 cut(s)	657, 843
SatI	GCNGC	7 cut(s)	205, 216, 753, 1575, 1661, 2778, 3513
Sau96I	GGNCC	3 cut(s)	2427, 2468, 2725
SchI	GAGTC	8 cut(s)	365, 373, 1313, 1933, 2171, 3773, 3817, 3878
ScrFI	CCNGG	8 cut(s)	327, 1262, 1308, 1529, 2342, 2513, 3576, 3843
SduI	GDGCHC	4 cut(s)	472, 807, 2713, 3853
SexAI	ACCWGGT	1 cut(s)	1306
SfaNI	GCATC	4 cut(s)	1567, 1591, 1909, 3175
SfcI	CTRYAG	1 cut(s)	2694
Sfr274I	CTCGAG	1 cut(s)	2330
SfuI	TTCGAA	3 cut(s)	2821, 3387, 3410
SgsI	GGCGCGCC	1 cut(s)	2336
SinI	GGWCC	3 cut(s)	2427, 2468, 2725
SlaI	CTCGAG	1 cut(s)	2330
SmiMI	CAYNNNNRTG	4 cut(s)	351, 390, 441, 2148
SmlI	CTYRAG	5 cut(s)	339, 2159, 2330, 3101, 3479
SmoI	CTYRAG	5 cut(s)	339, 2159, 2330, 3101, 3479
SseBI	AGGCCT	1 cut(s)	3999
SspI	AATATT	2 cut(s)	3088, 3313
SspMI	CTAG	9 cut(s)	783, 2282, 2630, 3108, 3182, 3683, 3827, 3909, 4101
SstI	GAGCTC	2 cut(s)	472, 807
StuI	AGGCCT	1 cut(s)	3999
StyD4I	CCNGG	8 cut(s)	325, 1260, 1306, 1527, 2340, 2511, 3574, 3841
StyI	CCWWGG	4 cut(s)	192, 1050, 2704, 3569
TaiI	ACGT	7 cut(s)	174, 1699, 2035, 3021, 3130, 3310, 3850
TatI	WGTACW	3 cut(s)	343, 670, 2278
TauI	GCSGC	1 cut(s)	207
TfiI	GAWTC	9 cut(s)	1417, 2019, 2208, 2414, 2666, 3200, 3407, 3487, 3959
TscAI	CASTG	7 cut(s)	126, 392, 1205, 2523, 2911, 3000, 4015
TseFI	GTSAC	4 cut(s)	112, 198, 368, 434
TseI	GCWGC	6 cut(s)	215, 752, 1574, 1660, 2777, 3512
Tsp45I	GTSAC	4 cut(s)	112, 198, 368, 434
TspGWI	ACGGA	7 cut(s)	417, 1742, 1795, 2753, 3327, 3477, 4089
TspRI	CASTG	7 cut(s)	126, 392, 1205, 2523, 2911, 3000, 4015
Tth111I	GACNNNGTC	2 cut(s)	1583, 4010
VpaK11BI	GGWCC	3 cut(s)	2427, 2468, 2725
VspI	ATTAAT	1 cut(s)	1955
XagI	CCTNNNNNAGG	2 cut(s)	2318, 3716
XapI	RAATTY	9 cut(s)	58, 272, 675, 918, 1833, 2973, 3242, 3351, 3466
XceI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2941
XhoI	CTCGAG	1 cut(s)	2330
XmiI	GTMKAC	3 cut(s)	719, 1793, 4044
XmnI	GAANNNNTTC	3 cut(s)	2034, 2973, 3393
XspI	CTAG	9 cut(s)	783, 2282, 2630, 3108, 3182, 3683, 3827, 3909, 4101
ZraI	GACGTC	1 cut(s)	3019
Zsp2I	ATGCAT	2 cut(s)	1560, 3745

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.