pycom13g11980
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004722
Physical Location & Seq
Forward (+)
8292689 .. 8293636
948 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 507 bp
ATGGCTTCTCGGCCCCAATCTCAGCCACCGGCGCCGGCGCTATTGCCGCCCTCGACGACGTCGGTTCTGGATGCTCCACTAGGGGAAATCGGGTTCGAGGTTGAAGAGATAACCCCCAAAAAGGTCTCTGGTCGCCTCCATGTCACCGAAAAGTGCGTCCAGCCGTTCAAGGTGCTACATGGGGGGATATCAGCACTCATCTCTGAGGCCCTGGCTAGCATGGGAGCTCACATGGCCTCTGGTTTCCAAAGGGTGGCCGGGATTCACCTCAGCATCAGCCATTTGAAGCCCGCCCAGCTCGGCGACCATATCTTTGCTGAAGCCTCCCCGGTCTACGTCGGCAGAACCATTCAGGTTTGGGAGGTGAGACTATGGAAGATTGACCCTTCAAACTCAGACATCAAATCCATCGTATCATCATCAAGAGTTACTCTTTTAAGCAACATGCCTGTGCCAGAGCACGCGAAAGACGCTGGTGAAAAGCTCAGGAAGTATGCCAAATTATAA

Protein Analysis

169

Amino Acids

18.09

Weight (kDa)

9.1

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 57 - 129 2e-16 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 505
AatII GACGTC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 253
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 464
AciI CCGC 2 cut(s) 47, 291
AcoI YGGCCR 1 cut(s) 255
AcuI CTGAAG 1 cut(s) 339
AcyI GRCGYC 2 cut(s) 32, 59
AfiI CCNNNNNNNGG 2 cut(s) 121, 253
AgsI TTSAA 4 cut(s) 104, 169, 286, 390
AjnI CCWGG 1 cut(s) 210
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
AluBI AGCT 3 cut(s) 227, 298, 484
AluI AGCT 3 cut(s) 227, 298, 484
Alw21I GWGCWC 2 cut(s) 229, 462
Alw26I GTCTC 2 cut(s) 130, 361
AoxI GGCC 4 cut(s) 11, 207, 234, 255
ArsI GACNNNNNNTTYG 4 cut(s) 141, 173, 296, 328
AspLEI GCGC 2 cut(s) 34, 40
AspS9I GGNCC 2 cut(s) 12, 208
AsuC2I CCSGG 2 cut(s) 259, 329
AsuHPI GGTGA 4 cut(s) 136, 257, 376, 488
AsuNHI GCTAGC 1 cut(s) 215
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 229
Bbv12I GWGCWC 2 cut(s) 229, 462
BbvCI CCTCAGC 1 cut(s) 269
BccI CCATC 1 cut(s) 416
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 1 cut(s) 212
BcnI CCSGG 2 cut(s) 259, 329
BcoDI GTCTC 2 cut(s) 130, 361
BfaI CTAG 2 cut(s) 80, 216
BfoI RGCGCY 2 cut(s) 35, 41
BisI GCNGC 1 cut(s) 47
BlsI GCNGC 1 cut(s) 48
Bme1390I CCNGG 3 cut(s) 212, 259, 329
BmgT120I GGNCC 2 cut(s) 12, 208
BmiI GGNNCC 2 cut(s) 14, 33
BmrFI CCNGG 3 cut(s) 212, 259, 329
BmsI GCATC 2 cut(s) 61, 282
BmtI GCTAGC 1 cut(s) 219
Bpu10I CCTNAGC 2 cut(s) 269, 485
BpuMI CCSGG 2 cut(s) 259, 329
BsaHI GRCGYC 2 cut(s) 32, 59
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 210, 327
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 253
Bse118I RCCGGY 2 cut(s) 28, 34
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 2 cut(s) 210, 327
BseGI GGATG 1 cut(s) 76
BseLI CCNNNNNNNGG 2 cut(s) 121, 253
BseMII CTCAG 5 cut(s) 35, 195, 283, 408, 499
BseYI CCCAGC 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 4 cut(s) 13, 209, 236, 257
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 2 cut(s) 229, 462
BsiSI CCGG 4 cut(s) 29, 35, 258, 329
BslI CCNNNNNNNGG 2 cut(s) 121, 253
BsmAI GTCTC 2 cut(s) 130, 361
BsnI GGCC 4 cut(s) 13, 209, 236, 257
Bso31I GGTCTC 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 229, 462
BspACI CCGC 2 cut(s) 47, 291
BspANI GGCC 4 cut(s) 13, 209, 236, 257
BspCNI CTCAG 5 cut(s) 34, 196, 282, 407, 498
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 2 cut(s) 14, 33
BspOI GCTAGC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 130
BsrFI RCCGGY 2 cut(s) 28, 34
BssAI RCCGGY 2 cut(s) 28, 34
BssECI CCNNGG 2 cut(s) 210, 327
BssNI GRCGYC 2 cut(s) 32, 59
Bst2UI CCWGG 1 cut(s) 212
Bst6I CTCTTC 1 cut(s) 99
BstACI GRCGYC 2 cut(s) 32, 59
BstC8I GCNNGC 4 cut(s) 36, 217, 291, 462
BstDEI CTNAG 5 cut(s) 21, 204, 269, 394, 485
BstF5I GGATG 1 cut(s) 76
BstFNI CGCG 1 cut(s) 464
BstH2I RGCGCY 2 cut(s) 35, 41
BstHHI GCGC 2 cut(s) 34, 40
BstMAI GTCTC 2 cut(s) 130, 361
BstMWI GCNNNNNNNGC 5 cut(s) 31, 46, 233, 295, 470
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 3 cut(s) 210, 257, 327
BstUI CGCG 1 cut(s) 464
BsuRI GGCC 4 cut(s) 13, 209, 236, 257
BtsCI GGATG 1 cut(s) 76
Cac8I GCNNGC 4 cut(s) 36, 217, 291, 462
CfoI GCGC 2 cut(s) 34, 40
Cfr10I RCCGGY 2 cut(s) 28, 34
Cfr13I GGNCC 2 cut(s) 12, 208
CseI GACGC 2 cut(s) 145, 479
CviAII CATG 5 cut(s) 140, 179, 220, 232, 445
DdeI CTNAG 5 cut(s) 21, 204, 269, 394, 485
DinI GGCGCC 1 cut(s) 33
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 229
Eco31I GGTCTC 1 cut(s) 130
Eco32I GATATC 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 210
EcoRV GATATC 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 229
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
FaeI CATG 5 cut(s) 143, 182, 223, 235, 448
FaiI YATR 9 cut(s) 141, 180, 221, 233, 309, 373, 446, 495, 505
FatI CATG 5 cut(s) 139, 178, 219, 231, 444
FauI CCCGC 1 cut(s) 298
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 1 cut(s) 47
FokI GGATG 1 cut(s) 83
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 47
FspBI CTAG 2 cut(s) 80, 216
GlaI GCGC 2 cut(s) 33, 39
GluI GCNGC 1 cut(s) 47
GsaI CCCAGC 1 cut(s) 298
HaeII RGCGCY 2 cut(s) 35, 41
HaeIII GGCC 4 cut(s) 13, 209, 236, 257
HapII CCGG 4 cut(s) 29, 35, 258, 329
HgaI GACGC 2 cut(s) 145, 479
HhaI GCGC 2 cut(s) 34, 40
Hin1I GRCGYC 2 cut(s) 32, 59
Hin1II CATG 5 cut(s) 143, 182, 223, 235, 448
Hin6I GCGC 2 cut(s) 32, 38
HinP1I GCGC 2 cut(s) 32, 38
HinfI GANTC 1 cut(s) 262
HpaII CCGG 4 cut(s) 29, 35, 258, 329
HphI GGTGA 4 cut(s) 136, 257, 376, 488
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 2 cut(s) 205, 397
Hpy188III TCNNGA 3 cut(s) 68, 423, 487
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 4 cut(s) 58, 61, 64, 341
HpyAV CCTTC 1 cut(s) 396
HpyCH4IV ACGT 2 cut(s) 59, 336
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 46, 233, 295, 470
HpyF3I CTNAG 5 cut(s) 21, 204, 269, 394, 485
HpySE526I ACGT 2 cut(s) 59, 336
Hsp92I GRCGYC 2 cut(s) 32, 59
Hsp92II CATG 5 cut(s) 143, 182, 223, 235, 448
HspAI GCGC 2 cut(s) 32, 38
KasI GGCGCC 1 cut(s) 31
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
LmnI GCTCC 2 cut(s) 79, 224
LweI GCATC 2 cut(s) 61, 282
MaeI CTAG 2 cut(s) 80, 216
MaeII ACGT 2 cut(s) 59, 336
MaeIII GTNAC 2 cut(s) 142, 427
MboII GAAGA 2 cut(s) 116, 388
MhlI GDGCHC 2 cut(s) 229, 462
MluCI AATT 1 cut(s) 500
Mly113I GGCGCC 1 cut(s) 32
MnlI CCTC 8 cut(s) 61, 91, 146, 199, 247, 278, 334, 355
MreI CGCCGGCG 1 cut(s) 34
MroNI GCCGGC 1 cut(s) 34
MseI TTAA 1 cut(s) 437
MslI CAYNNNNRTG 1 cut(s) 449
MspI CCGG 4 cut(s) 29, 35, 258, 329
MspR9I CCNGG 3 cut(s) 212, 259, 329
MvaI CCWGG 1 cut(s) 212
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 5 cut(s) 31, 46, 233, 295, 470
NaeI GCCGGC 1 cut(s) 36
NarI GGCGCC 1 cut(s) 32
NciI CCSGG 2 cut(s) 259, 329
NgoMIV GCCGGC 1 cut(s) 34
NheI GCTAGC 1 cut(s) 215
NlaIII CATG 5 cut(s) 143, 182, 223, 235, 448
NlaIV GGNNCC 2 cut(s) 14, 33
NmeAIII GCCGAG 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 142
NspI RCATGY 1 cut(s) 448
PdiI GCCGGC 1 cut(s) 36
PfeI GAWTC 1 cut(s) 262
PflFI GACNNNGTC 1 cut(s) 58
PflMI CCANNNNNTGG 1 cut(s) 253
PkrI GCNGC 1 cut(s) 48
PluTI GGCGCC 1 cut(s) 35
PsiI TTATAA 1 cut(s) 505
Psp124BI GAGCTC 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 14, 33
PspPI GGNCC 2 cut(s) 12, 208
PsyI GACNNNGTC 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 449
SacI GAGCTC 1 cut(s) 229
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 1 cut(s) 47
Sau96I GGNCC 2 cut(s) 12, 208
ScrFI CCNGG 3 cut(s) 212, 259, 329
SduI GDGCHC 2 cut(s) 229, 462
SfaNI GCATC 2 cut(s) 61, 282
SfoI GGCGCC 1 cut(s) 33
SgrAI CRCCGGYG 2 cut(s) 28, 34
SmiMI CAYNNNNRTG 1 cut(s) 449
Sse9I AATT 1 cut(s) 500
SsiI CCGC 2 cut(s) 47, 291
SspDI GGCGCC 1 cut(s) 31
SspMI CTAG 2 cut(s) 80, 216
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 3 cut(s) 210, 257, 327
TaiI ACGT 2 cut(s) 62, 339
TaqI TCGA 2 cut(s) 53, 96
TasI AATT 1 cut(s) 500
TauI GCSGC 1 cut(s) 49
TfiI GAWTC 1 cut(s) 262
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TseFI GTSAC 1 cut(s) 142
Tsp45I GTSAC 1 cut(s) 142
Tth111I GACNNNGTC 1 cut(s) 58
Van91I CCANNNNNTGG 1 cut(s) 253
XceI RCATGY 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 2 cut(s) 80, 216
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.