pycom13g15200

Inosine-uridine preferring nucleoside hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001810
Physical Location & Seq
Forward (+)
11074895 .. 11079813
4919 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1677 bp
ATGTTGTTGCAGAAGCAGAAACGTTTTGATTGGTTAGTGCAGATGCTAGTTGTTGTGGGATCAGTATTGGTTGTGATTGGAGCTAGCAATTTGCGGACTGTGGAAGGCAGGCCTCACCGGATTCTTTTCGATACAGATGTTGATACCGATGATTTCTTCGGTCTCTTGTACATCTTGAAACTTAACAGATCAGAGTTTGAGTTGGAGGCAGTGACTGTAAATGCAAATGCTTGGACCAATCCTGGACATGCTGTGCATCAAATCTATGACATTTTATACATGATGGGGCGTGATGACGTGGCAGTTGGAGTGGGTGGTGAAGGTGGGATCAAAGAAGATGGTACAATTTTGCCAAATGTCGGTGGATATCTTCCAATTATTGAACAGGGGACTACGACAGCAGGAGGATGCAGATATAGACAAGCCATTCCTGTAGGTGCTAGAGGTCGATTGGATGTTGATTCTAATTTTGGCCTCAGAAAATCTTTCCTCCCCCAGGGGAGCAGGAGATATACTCCCTTTCGACAACCTACTGCTCAGCAAGTGATGATTGACAACATATCTGCAGGTCCCATTACTGTATTTCTGATAGGAGCACACACAAACTTTGCCCTTTTCCTTATGAACAATCCACACCTAAAGAAGAATATTGAGCACATTTATGTCATGGGTGGTGGTGTGAGATCGAAAAACCCTACTGGTTGTTGCCCGGAAAATTCTACGCCCTGTGTTCCTCGGCAGTGTAGTGATCCTGGCAATGTCTTCACAGACTACACAAGTAATCCTTATGCTGAGTTCAATTTCCTTGGTGATCCCTTTGCTGCATACCAGGTTATACATTCTGGGATTTCAGTCACCCTTGTTCCTCTGGATGCAACAAACACCATTCCCGTTAGTCAAAACTTCTTTGAGGCCTTTGAGAAGAGCCAGAACACATATGAGGCACAATATGTCTTCCAGTCCTTGAAAATGGCTCGTGATACTTGGTTTGACAAACAATTCTACACGAGCTATTTCATGTGGGATTCATTTACAGCTGGTGTGGCGGTATCAATCATGCGAAACAACTTGAATAACCCTAATGGAGAAAATGAATTTGCGGAAATGGAGTATATGAATATAACTGTGATTACTTCAAATGAACCTTATGGAGTAACTGATGGCTCTAATCCTTTCTTTGATGGCGTCAAAGTTCCAAAGTTCAACTTAGACAAGAATGGTGTGCATAGTGGTCATGTTCAAAAGGGACTAAGAGATCCATTTTGCATTGGGGAGAATGGCAAAGGGAAATGCAGGGATGGTTATACAGAAGAAGTAACAGGATCAGAGGCTGTGTCTGTGCTTGTTGCTACAAAAGCCAAACCAAATCAGGACCCTCAAAGCCCACTTAACAGAGAATTCTTCGTAAGCTTCCTGGAGGTTCTGAACAACCCCAAACAGAAAGGGAGATTCAATTTAACCACACAATTTCCATTTTACAAGGAAGTGACATATAAACCAGAATTTGGAAACAAAAAACTTGGTAAACCTGTTGTCTTTGACATGGATATGAGTGCTGGAGACTTTGCAGCTCTCTTCTACCTCCTCAAAGTACCTATTCAAGTGATTAATCTCAGGGTAGTTTTTTGCTTCTTCTTCTCTTTTTCCCAACAGGCTGGGCAAATGCTGCAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IU_nuc_hydro

Protein Analysis

559

Amino Acids

62.32

Weight (kDa)

6.04

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IU_nuc_hydro PF01156 41 - 384 7.1e-36 Inosine-uridine preferring nucleoside hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 557
AccB7I CCANNNNNTGG 1 cut(s) 1505
AciI CCGC 3 cut(s) 94, 1046, 1100
AclI AACGTT 1 cut(s) 22
AclWI GGATC 6 cut(s) 67, 335, 743, 806, 1250, 1330
AcsI RAATTY 4 cut(s) 715, 1094, 1397, 1502
AcyI GRCGYC 1 cut(s) 1185
AfaI GTAC 3 cut(s) 170, 343, 1593
AfiI CCNNNNNNNGG 3 cut(s) 359, 496, 1505
AjiI CACGTC 1 cut(s) 298
AjnI CCWGG 5 cut(s) 241, 495, 751, 828, 1413
AluBI AGCT 5 cut(s) 83, 1011, 1037, 1410, 1571
AluI AGCT 5 cut(s) 83, 1011, 1037, 1410, 1571
Alw21I GWGCWC 2 cut(s) 598, 657
Alw26I GTCTC 2 cut(s) 167, 1554
AlwI GGATC 6 cut(s) 67, 335, 743, 806, 1250, 1330
AlwNI CAGNNNCTG 2 cut(s) 215, 1331
AoxI GGCC 3 cut(s) 110, 472, 912
ApeKI GCWGC 3 cut(s) 821, 1568, 1666
ApoI RAATTY 4 cut(s) 715, 1094, 1397, 1502
AseI ATTAAT 1 cut(s) 1608
AspS9I GGNCC 3 cut(s) 234, 569, 1372
AsuC2I CCSGG 1 cut(s) 710
AsuHPI GGTGA 4 cut(s) 107, 329, 821, 847
AsuNHI GCTAGC 1 cut(s) 83
AvaII GGWCC 3 cut(s) 234, 569, 1372
BaeI ACNNNNGTAYC 2 cut(s) 123, 156
BauI CACGAG 2 cut(s) 975, 1006
BbsI GAAGAC 2 cut(s) 754, 946
Bbv12I GWGCWC 2 cut(s) 598, 657
BbvI GCAGC 3 cut(s) 808, 1580, 1653
BccI CCATC 5 cut(s) 277, 332, 1154, 1175, 1292
BciT130I CCWGG 5 cut(s) 243, 497, 753, 830, 1415
BcnI CCSGG 1 cut(s) 710
BcoDI GTCTC 2 cut(s) 167, 1554
BfaI CTAG 3 cut(s) 47, 84, 441
BfmI CTRYAG 2 cut(s) 432, 564
BfuAI ACCTGC 1 cut(s) 557
BisI GCNGC 3 cut(s) 822, 1569, 1667
BlpI GCTNAGC 1 cut(s) 537
BlsI GCNGC 3 cut(s) 823, 1570, 1668
Bme1390I CCNGG 6 cut(s) 243, 497, 710, 753, 830, 1415
Bme18I GGWCC 3 cut(s) 234, 569, 1372
BmgBI CACGTC 1 cut(s) 298
BmgT120I GGNCC 3 cut(s) 234, 569, 1372
BmiI GGNNCC 2 cut(s) 571, 1374
BmrFI CCNGG 6 cut(s) 243, 497, 710, 753, 830, 1415
BmsI GCATC 4 cut(s) 33, 265, 398, 862
BmtI GCTAGC 1 cut(s) 87
BpiI GAAGAC 2 cut(s) 754, 946
BplI GAGNNNNNCTC 2 cut(s) 499, 531
BpmI CTGGAG 2 cut(s) 1436, 1578
Bpu1102I GCTNAGC 1 cut(s) 537
BpuMI CCSGG 1 cut(s) 710
BsaHI GRCGYC 1 cut(s) 1185
BsaI GGTCTC 1 cut(s) 167
BsaJI CCNNGG 4 cut(s) 495, 496, 734, 805
BsaWI WCCGGW 1 cut(s) 117
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 496, 1505
Bse1I ACTGG 2 cut(s) 703, 958
Bse3DI GCAATG 1 cut(s) 763
BseBI CCWGG 5 cut(s) 243, 497, 753, 830, 1415
BseDI CCNNGG 4 cut(s) 495, 496, 734, 805
BseGI GGATG 4 cut(s) 413, 460, 877, 1303
BseLI CCNNNNNNNGG 3 cut(s) 359, 496, 1505
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 4 cut(s) 490, 551, 783, 1627
BseNI ACTGG 2 cut(s) 703, 958
BseRI GAGGAG 1 cut(s) 1574
BseXI GCAGC 3 cut(s) 808, 1580, 1653
BseYI CCCAGC 1 cut(s) 1655
BsgI GTGCAG 1 cut(s) 59
BshFI GGCC 3 cut(s) 112, 474, 914
BsiHKAI GWGCWC 2 cut(s) 598, 657
BsiSI CCGG 2 cut(s) 118, 710
BslFI GGGAC 3 cut(s) 403, 555, 1260
BslI CCNNNNNNNGG 3 cut(s) 359, 496, 1505
BsmAI GTCTC 2 cut(s) 167, 1554
BsmFI GGGAC 3 cut(s) 403, 555, 1260
BsnI GGCC 3 cut(s) 112, 474, 914
Bso31I GGTCTC 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 598, 657
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 8 cut(s) 59, 188, 327, 683, 748, 811, 1255, 1322
Bsp1720I GCTNAGC 1 cut(s) 537
BspACI CCGC 3 cut(s) 94, 1046, 1100
BspANI GGCC 3 cut(s) 112, 474, 914
BspCNI CTCAG 4 cut(s) 489, 550, 784, 1626
BspLI GGNNCC 2 cut(s) 571, 1374
BspMAI CTGCAG 1 cut(s) 568
BspMI ACCTGC 1 cut(s) 557
BspOI GCTAGC 1 cut(s) 87
BspPI GGATC 6 cut(s) 67, 335, 743, 806, 1250, 1330
BspQI GCTCTTC 1 cut(s) 917
BspTNI GGTCTC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 763
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 2 cut(s) 703, 958
BssECI CCNNGG 4 cut(s) 495, 496, 734, 805
BssMI GATC 8 cut(s) 59, 188, 327, 683, 748, 811, 1255, 1322
BssNI GRCGYC 1 cut(s) 1185
BssSI CACGAG 2 cut(s) 975, 1006
BssT1I CCWWGG 1 cut(s) 805
Bst2BI CACGAG 2 cut(s) 975, 1006
Bst2UI CCWGG 5 cut(s) 243, 497, 753, 830, 1415
Bst4CI ACNGT 4 cut(s) 100, 217, 580, 1126
Bst6I CTCTTC 2 cut(s) 917, 1580
BstACI GRCGYC 1 cut(s) 1185
BstAPI GCANNNNNTGC 1 cut(s) 1666
BstAUI TGTACA 1 cut(s) 168
BstC8I GCNNGC 2 cut(s) 85, 110
BstDEI CTNAG 6 cut(s) 476, 537, 792, 1207, 1250, 1613
BstENI CCTNNNNNAGG 1 cut(s) 494
BstF5I GGATG 4 cut(s) 413, 460, 877, 1303
BstKTI GATC 8 cut(s) 62, 191, 330, 686, 751, 814, 1258, 1325
BstMAI GTCTC 2 cut(s) 167, 1554
BstMBI GATC 8 cut(s) 59, 188, 327, 683, 748, 811, 1255, 1322
BstMWI GCNNNNNNNGC 3 cut(s) 1043, 1355, 1666
BstNI CCWGG 5 cut(s) 243, 497, 753, 830, 1415
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 6 cut(s) 241, 495, 708, 751, 828, 1413
BstSFI CTRYAG 2 cut(s) 432, 564
BstV1I GCAGC 3 cut(s) 808, 1580, 1653
BstV2I GAAGAC 2 cut(s) 754, 946
BstX2I RGATCY 1 cut(s) 1255
BstXI CCANNNNNNTGG 1 cut(s) 1655
BstYI RGATCY 1 cut(s) 1255
BsuRI GGCC 3 cut(s) 112, 474, 914
BtrI CACGTC 1 cut(s) 298
BtsCI GGATG 4 cut(s) 413, 460, 877, 1303
BtsI GCAGTG 2 cut(s) 216, 746
BtsIMutI CAGTG 2 cut(s) 216, 746
BveI ACCTGC 1 cut(s) 557
Cac8I GCNNGC 2 cut(s) 85, 110
CaiI CAGNNNCTG 2 cut(s) 215, 1331
Cfr13I GGNCC 3 cut(s) 234, 569, 1372
CseI GACGC 1 cut(s) 1174
CsiI ACCWGGT 1 cut(s) 828
Csp6I GTAC 3 cut(s) 169, 342, 1592
CviAII CATG 7 cut(s) 248, 280, 667, 1018, 1057, 1235, 1543
CviQI GTAC 3 cut(s) 169, 342, 1592
DdeI CTNAG 6 cut(s) 476, 537, 792, 1207, 1250, 1613
DpnI GATC 8 cut(s) 61, 190, 329, 685, 750, 813, 1257, 1324
DpnII GATC 8 cut(s) 59, 188, 327, 683, 748, 811, 1255, 1322
Eam1104I CTCTTC 2 cut(s) 917, 1580
EarI CTCTTC 2 cut(s) 917, 1580
Eco130I CCWWGG 1 cut(s) 805
Eco147I AGGCCT 2 cut(s) 112, 914
Eco31I GGTCTC 1 cut(s) 167
Eco32I GATATC 1 cut(s) 368
Eco47I GGWCC 3 cut(s) 234, 569, 1372
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoO109I RGGNCCY 2 cut(s) 569, 1372
EcoRI GAATTC 1 cut(s) 1397
EcoRII CCWGG 5 cut(s) 241, 495, 751, 828, 1413
EcoRV GATATC 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 805
ErhI CCWWGG 1 cut(s) 805
FaeI CATG 7 cut(s) 251, 283, 670, 1021, 1060, 1238, 1546
FalI AAGNNNNNCTT 4 cut(s) 769, 801, 1190, 1222
FaqI GGGAC 3 cut(s) 403, 555, 1260
FatI CATG 7 cut(s) 247, 279, 666, 1017, 1056, 1234, 1542
FauNDI CATATG 1 cut(s) 937
Fnu4HI GCNGC 3 cut(s) 822, 1569, 1667
FokI GGATG 4 cut(s) 420, 467, 884, 1310
Fsp4HI GCNGC 3 cut(s) 822, 1569, 1667
FspBI CTAG 3 cut(s) 47, 84, 441
GluI GCNGC 3 cut(s) 822, 1569, 1667
GsaI CCCAGC 1 cut(s) 1659
GsuI CTGGAG 2 cut(s) 1436, 1578
HaeIII GGCC 3 cut(s) 112, 474, 914
HapII CCGG 2 cut(s) 118, 710
HgaI GACGC 1 cut(s) 1174
Hin1I GRCGYC 1 cut(s) 1185
Hin1II CATG 7 cut(s) 251, 283, 670, 1021, 1060, 1238, 1546
HindIII AAGCTT 1 cut(s) 1408
HinfI GANTC 4 cut(s) 121, 461, 1025, 1449
HpaII CCGG 2 cut(s) 118, 710
HphI GGTGA 4 cut(s) 107, 329, 821, 847
Hpy166II GTNNAC 1 cut(s) 1526
Hpy188I TCNGA 5 cut(s) 193, 479, 588, 1327, 1425
Hpy188III TCNNGA 4 cut(s) 175, 869, 977, 1370
Hpy8I GTNNAC 1 cut(s) 1526
HpyAV CCTTC 2 cut(s) 98, 314
HpyCH4III ACNGT 4 cut(s) 100, 217, 580, 1126
HpyCH4IV ACGT 2 cut(s) 22, 297
HpyF10VI GCNNNNNNNGC 3 cut(s) 1043, 1355, 1666
HpyF3I CTNAG 6 cut(s) 476, 537, 792, 1207, 1250, 1613
HpySE526I ACGT 2 cut(s) 22, 297
Hsp92I GRCGYC 1 cut(s) 1185
Hsp92II CATG 7 cut(s) 251, 283, 670, 1021, 1060, 1238, 1546
Kzo9I GATC 8 cut(s) 59, 188, 327, 683, 748, 811, 1255, 1322
LguI GCTCTTC 1 cut(s) 917
LmnI GCTCC 3 cut(s) 80, 501, 593
Lsp1109I GCAGC 3 cut(s) 808, 1580, 1653
LweI GCATC 4 cut(s) 33, 265, 398, 862
MabI ACCWGGT 1 cut(s) 828
MaeI CTAG 3 cut(s) 47, 84, 441
MaeII ACGT 2 cut(s) 22, 297
MaeIII GTNAC 5 cut(s) 211, 853, 1153, 1315, 1486
MalI GATC 8 cut(s) 61, 190, 329, 685, 750, 813, 1257, 1324
MboI GATC 8 cut(s) 59, 188, 327, 683, 748, 811, 1255, 1322
MflI RGATCY 1 cut(s) 1255
MhlI GDGCHC 2 cut(s) 598, 657
MmeI TCCRAC 2 cut(s) 183, 286
MseI TTAA 4 cut(s) 183, 1389, 1457, 1608
MslI CAYNNNNRTG 2 cut(s) 660, 1547
MspA1I CMGCKG 1 cut(s) 1037
MspI CCGG 2 cut(s) 118, 710
MspR9I CCNGG 6 cut(s) 243, 497, 710, 753, 830, 1415
MvaI CCWGG 5 cut(s) 243, 497, 753, 830, 1415
MwoI GCNNNNNNNGC 3 cut(s) 1043, 1355, 1666
NciI CCSGG 1 cut(s) 710
NdeI CATATG 1 cut(s) 937
NdeII GATC 8 cut(s) 59, 188, 327, 683, 748, 811, 1255, 1322
NheI GCTAGC 1 cut(s) 83
NlaIII CATG 7 cut(s) 251, 283, 670, 1021, 1060, 1238, 1546
NlaIV GGNNCC 2 cut(s) 571, 1374
NmeAIII GCCGAG 1 cut(s) 715
NmuCI GTSAC 3 cut(s) 211, 853, 1486
NspI RCATGY 1 cut(s) 251
PasI CCCWGGG 1 cut(s) 496
PceI AGGCCT 2 cut(s) 112, 914
PciSI GCTCTTC 1 cut(s) 917
PfeI GAWTC 4 cut(s) 121, 461, 1025, 1449
PflMI CCANNNNNTGG 1 cut(s) 1505
PfoI TCCNGGA 2 cut(s) 241, 1413
PkrI GCNGC 3 cut(s) 823, 1570, 1668
PpuMI RGGWCCY 2 cut(s) 569, 1372
PshBI ATTAAT 1 cut(s) 1608
Psp1406I AACGTT 1 cut(s) 22
Psp5II RGGWCCY 2 cut(s) 569, 1372
Psp6I CCWGG 5 cut(s) 241, 495, 751, 828, 1413
PspFI CCCAGC 1 cut(s) 1655
PspGI CCWGG 5 cut(s) 241, 495, 751, 828, 1413
PspN4I GGNNCC 2 cut(s) 571, 1374
PspPI GGNCC 3 cut(s) 234, 569, 1372
PspPPI RGGWCCY 2 cut(s) 569, 1372
PstI CTGCAG 1 cut(s) 568
PstNI CAGNNNCTG 2 cut(s) 215, 1331
PsuI RGATCY 1 cut(s) 1255
PvuII CAGCTG 1 cut(s) 1037
RsaI GTAC 3 cut(s) 170, 343, 1593
RsaNI GTAC 3 cut(s) 169, 342, 1592
RseI CAYNNNNRTG 2 cut(s) 660, 1547
SapI GCTCTTC 1 cut(s) 917
SaqAI TTAA 4 cut(s) 183, 1389, 1457, 1608
SatI GCNGC 3 cut(s) 822, 1569, 1667
Sau3AI GATC 8 cut(s) 59, 188, 327, 683, 748, 811, 1255, 1322
Sau96I GGNCC 3 cut(s) 234, 569, 1372
ScrFI CCNGG 6 cut(s) 243, 497, 710, 753, 830, 1415
SduI GDGCHC 2 cut(s) 598, 657
SexAI ACCWGGT 1 cut(s) 828
SfaNI GCATC 4 cut(s) 33, 265, 398, 862
SfcI CTRYAG 2 cut(s) 432, 564
SinI GGWCC 3 cut(s) 234, 569, 1372
SmiMI CAYNNNNRTG 2 cut(s) 660, 1547
SseBI AGGCCT 2 cut(s) 112, 914
SsiI CCGC 3 cut(s) 94, 1046, 1100
SspI AATATT 1 cut(s) 649
SspMI CTAG 3 cut(s) 47, 84, 441
StuI AGGCCT 2 cut(s) 112, 914
StyD4I CCNGG 6 cut(s) 241, 495, 708, 751, 828, 1413
StyI CCWWGG 1 cut(s) 805
TaaI ACNGT 4 cut(s) 100, 217, 580, 1126
TaiI ACGT 2 cut(s) 25, 300
TaqI TCGA 4 cut(s) 129, 448, 523, 686
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 1 cut(s) 168
TfiI GAWTC 4 cut(s) 121, 461, 1025, 1449
Tru1I TTAA 4 cut(s) 183, 1389, 1457, 1608
Tru9I TTAA 4 cut(s) 183, 1389, 1457, 1608
TscAI CASTG 2 cut(s) 216, 746
TseFI GTSAC 3 cut(s) 211, 853, 1486
TseI GCWGC 3 cut(s) 821, 1568, 1666
Tsp45I GTSAC 3 cut(s) 211, 853, 1486
TspDTI ATGAA 6 cut(s) 638, 1006, 1017, 1107, 1130, 1155
TspRI CASTG 2 cut(s) 216, 746
Van91I CCANNNNNTGG 1 cut(s) 1505
VpaK11BI GGWCC 3 cut(s) 234, 569, 1372
VspI ATTAAT 1 cut(s) 1608
XagI CCTNNNNNAGG 1 cut(s) 494
XapI RAATTY 4 cut(s) 715, 1094, 1397, 1502
XceI RCATGY 1 cut(s) 251
XspI CTAG 3 cut(s) 47, 84, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.