pycom13g18390

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006926
Physical Location & Seq
Forward (+)
14507470 .. 14509209
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGGAAGAGTTCATGGATCTCAAACTTGTTGGGCATGCAGACTCGCATGAATCTAACAAATACGACCGAGCCAAAGAGCTAAAAGCTTTCGATGATACGATGGCTGGTGTCAAAGGCCTAGTAGATGCTGGCACAGTTCAAGTACCCAAGATATTCATAGAGCCACCCAACGATCTCACCCATTTTACCACGTGTCACCAACCCAACCTGCTAATTCCTGTCATTGATCTCAGTGGCATCAATGGTAGTGATGAGATCCACAAGCAGATTGTAGATGATGTTCGGACTGCGTCCGAAACATGGGGATTCTTCCAAGTGGTCAATCATGGAGTCCCACTGAGTGTGCTGGAAAATATGATCCAAGGAATAGTTGAGTTTCATGAGCAGGATCTTGATGCGAAGAAAGAGTTCTATTCCCGTGATCTAGGGAGAACCGTCCATTTCCTTAGCAACTATGATCTGTACCAATCAAAAGCAGCTAATTGGAGGGATACTTTGTCCTTTACCAGGTCTGCTGTTGATCATGATCCCAAACAGCTTCCTTCAGTCTGTAGATGCAACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

189

Amino Acids

21.27

Weight (kDa)

5.15

Isoelectric Point (pI)

31.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 72 - 178 1.3e-19 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 216
AclWI GGATC 5 cut(s) 24, 250, 352, 396, 521
AcuI CTGAAG 1 cut(s) 528
AcvI CACGTG 1 cut(s) 192
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 2 cut(s) 144, 464
AfiI CCNNNNNNNGG 2 cut(s) 300, 507
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 1 cut(s) 140
AjnI CCWGG 1 cut(s) 506
AluBI AGCT 4 cut(s) 79, 86, 479, 538
AluI AGCT 4 cut(s) 79, 86, 479, 538
AlwI GGATC 5 cut(s) 24, 250, 352, 396, 521
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 476
Asp700I GAANNNNTTC 1 cut(s) 407
AsuHPI GGTGA 2 cut(s) 169, 188
BbrPI CACGTG 1 cut(s) 192
BbvI GCAGC 1 cut(s) 488
BccI CCATC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 508
BciVI GTATCC 1 cut(s) 484
BclI TGATCA 1 cut(s) 520
BfaI CTAG 2 cut(s) 119, 425
BfmI CTRYAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 216
BfuI GTATCC 1 cut(s) 484
BisI GCNGC 1 cut(s) 477
BlsI GCNGC 1 cut(s) 478
Bme1390I CCNGG 1 cut(s) 508
BmrFI CCNGG 1 cut(s) 508
BmsI GCATC 4 cut(s) 115, 246, 385, 545
Bpu10I CCTNAGC 1 cut(s) 446
BsaAI YACGTR 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 300, 507
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 1 cut(s) 508
BseDI CCNNGG 1 cut(s) 361
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 2 cut(s) 300, 507
BseMII CTCAG 2 cut(s) 244, 329
BseXI GCAGC 1 cut(s) 488
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 1 cut(s) 117
BsiEI CGRYCG 1 cut(s) 67
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 2 cut(s) 300, 507
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 1 cut(s) 117
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 243, 330
BspHI TCATGA 2 cut(s) 379, 523
BspMI ACCTGC 1 cut(s) 216
BspPI GGATC 5 cut(s) 24, 250, 352, 396, 521
BssECI CCNNGG 1 cut(s) 361
BssT1I CCWWGG 1 cut(s) 361
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 2 cut(s) 136, 436
BstBAI YACGTR 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 36, 130
BstDEI CTNAG 3 cut(s) 230, 338, 446
BstENI CCTNNNNNAGG 1 cut(s) 505
BstMCI CGRYCG 1 cut(s) 67
BstNI CCWGG 1 cut(s) 508
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 506
BstSFI CTRYAG 1 cut(s) 550
BstV1I GCAGC 1 cut(s) 488
BstX2I RGATCY 3 cut(s) 16, 255, 388
BstYI RGATCY 3 cut(s) 16, 255, 388
BsuI GTATCC 1 cut(s) 484
BsuRI GGCC 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 238, 335
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 36, 130
CciI TCATGA 2 cut(s) 379, 523
CseI GACGC 1 cut(s) 279
CsiI ACCWGGT 1 cut(s) 506
Csp6I GTAC 2 cut(s) 143, 463
CviAII CATG 7 cut(s) 13, 35, 47, 300, 326, 380, 524
CviJI RGCY 8 cut(s) 71, 79, 86, 104, 117, 163, 479, 538
CviKI_1 RGCY 8 cut(s) 71, 79, 86, 104, 117, 163, 479, 538
CviQI GTAC 2 cut(s) 143, 463
DdeI CTNAG 3 cut(s) 230, 338, 446
DraIII CACNNNGTG 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 528
Eco72I CACGTG 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 505
EcoRII CCWGG 1 cut(s) 506
EcoT14I CCWWGG 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 361
FaeI CATG 7 cut(s) 16, 38, 50, 303, 329, 383, 527
FaqI GGGAC 1 cut(s) 317
FatI CATG 7 cut(s) 12, 34, 46, 299, 325, 379, 523
FbaI TGATCA 1 cut(s) 520
Fnu4HI GCNGC 1 cut(s) 477
Fsp4HI GCNGC 1 cut(s) 477
FspBI CTAG 2 cut(s) 119, 425
GluI GCNGC 1 cut(s) 477
HaeIII GGCC 1 cut(s) 117
HgaI GACGC 1 cut(s) 279
Hin1II CATG 7 cut(s) 16, 38, 50, 303, 329, 383, 527
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 4 cut(s) 41, 50, 306, 330
HphI GGTGA 2 cut(s) 169, 188
Hpy188I TCNGA 2 cut(s) 285, 295
Hpy188III TCNNGA 3 cut(s) 380, 392, 524
HpyAV CCTTC 1 cut(s) 552
HpyCH4III ACNGT 2 cut(s) 136, 436
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 2 cut(s) 38, 558
HpyF3I CTNAG 3 cut(s) 230, 338, 446
HpySE526I ACGT 1 cut(s) 191
Hsp92II CATG 7 cut(s) 16, 38, 50, 303, 329, 383, 527
Ksp22I TGATCA 1 cut(s) 520
LpnPI CCDG 8 cut(s) 90, 114, 221, 231, 332, 371, 493, 520
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 4 cut(s) 115, 246, 385, 545
MabI ACCWGGT 1 cut(s) 506
MaeI CTAG 2 cut(s) 119, 425
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 194
MboII GAAGA 3 cut(s) 17, 301, 412
MfeI CAATTG 1 cut(s) 562
MflI RGATCY 3 cut(s) 16, 255, 388
MluCI AATT 3 cut(s) 213, 481, 562
MlyI GAGTC 2 cut(s) 35, 339
MnlI CCTC 1 cut(s) 480
MroXI GAANNNNTTC 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 508
MunI CAATTG 1 cut(s) 562
MvaI CCWGG 1 cut(s) 508
NlaIII CATG 7 cut(s) 16, 38, 50, 303, 329, 383, 527
NmuCI GTSAC 1 cut(s) 194
NspI RCATGY 1 cut(s) 38
PaeI GCATGC 1 cut(s) 38
PagI TCATGA 2 cut(s) 379, 523
PceI AGGCCT 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 2 cut(s) 50, 306
PflFI GACNNNGTC 1 cut(s) 289
PkrI GCNGC 1 cut(s) 478
PleI GAGTC 2 cut(s) 35, 338
PmaCI CACGTG 1 cut(s) 192
PmlI CACGTG 1 cut(s) 192
PpsI GAGTC 2 cut(s) 35, 338
Ppu21I YACGTR 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 506
PspCI CACGTG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 506
PsrI GAACNNNNNNTAC 2 cut(s) 264, 296
PsuI RGATCY 3 cut(s) 16, 255, 388
PsyI GACNNNGTC 1 cut(s) 289
RsaI GTAC 2 cut(s) 144, 464
RsaNI GTAC 2 cut(s) 143, 463
SatI GCNGC 1 cut(s) 477
SchI GAGTC 2 cut(s) 35, 339
ScrFI CCNGG 1 cut(s) 508
SetI ASST 7 cut(s) 81, 88, 194, 210, 481, 512, 540
SexAI ACCWGGT 1 cut(s) 506
SfaNI GCATC 4 cut(s) 115, 246, 385, 545
SfcI CTRYAG 1 cut(s) 550
SphI GCATGC 1 cut(s) 38
Sse9I AATT 3 cut(s) 213, 481, 562
SseBI AGGCCT 1 cut(s) 117
SspMI CTAG 2 cut(s) 119, 425
StuI AGGCCT 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 506
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 2 cut(s) 136, 436
TaiI ACGT 1 cut(s) 194
TaqI TCGA 1 cut(s) 90
TaqII GACCGA 1 cut(s) 81
TasI AATT 3 cut(s) 213, 481, 562
TfiI GAWTC 2 cut(s) 50, 306
TscAI CASTG 2 cut(s) 238, 342
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 476
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 3 cut(s) 63, 145, 368
TspRI CASTG 2 cut(s) 238, 342
Tth111I GACNNNGTC 1 cut(s) 289
XagI CCTNNNNNAGG 1 cut(s) 505
XceI RCATGY 1 cut(s) 38
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 2 cut(s) 119, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.