pycom13g21170

membrane protein At1g06890-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007221
Physical Location & Seq
Reverse (-)
17422301 .. 17424748
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
ATGTTAAACTTCTTTGTGAGGAAAGGCGTTAGGAAGATTCTCAAGCGCAAGGACAGTGATGCTGGTGAAAGAGGAAGAGCTCTAGAAGACTTAAGATCTACCCTTTTTAATGAATTCCGCTCAGCCGATGGTGCAAAGCGTCAACAACAACAGCGATGTGGCCCTGCCACTGCTCTTACCTTCAATTTTGTAATTGCTGTCGGCATAATTTTCGTGAACAAATTGGTGCTCAAAGTTGTCGGGTTCCAGTTTCCTATATTTCTCACCTTTATCCACTATGTGGTAAGCTGGTTGTTGATGGCCATTCTAAATGCTCTATCTATCCTCCCTGCATGTCCCTCAAAATCGACCCGCTTATCTACATTATTTACTCTTGGCTTTGTTATGGCTTTCTCTACTGGCCTTGCTAATGTTAGCTTGAAGTATAATAGTGTGGGGTTCTATCAGATGTCTAAGATTGCGGTTACGCCATCAATTGTTCTCGCAGAATTTTTGTTGTACAACAAGAGAGTTTCTTTCTCGAAGGTTCTAGCACTTACGGTAGTATCGATTGGTGTTGCCGTGGCTACAGTCACTGATCGTTCAATTTCATCTCTTTGGTGCTTGTATAGCATTAGCATGGATAATACCAAGTGCAGTCAACAAAATTCTTTGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

220

Amino Acids

24.27

Weight (kDa)

10.11

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 60 - 197 6.4e-10 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 280
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 3 cut(s) 118, 352, 461
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 3 cut(s) 113, 488, 646
AdeI CACNNNGTG 1 cut(s) 280
AfaI GTAC 1 cut(s) 500
AfiI CCNNNNNNNGG 1 cut(s) 280
AflII CTTAAG 1 cut(s) 91
AgsI TTSAA 3 cut(s) 184, 421, 585
AluBI AGCT 3 cut(s) 80, 288, 417
AluI AGCT 3 cut(s) 80, 288, 417
Alw21I GWGCWC 2 cut(s) 82, 231
AlwNI CAGNNNCTG 1 cut(s) 575
AoxI GGCC 3 cut(s) 160, 300, 400
ApoI RAATTY 3 cut(s) 113, 488, 646
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 77, 256
BaeI ACNNNNGTAYC 2 cut(s) 528, 561
BalI TGGCCA 1 cut(s) 302
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 82, 231
BccI CCATC 3 cut(s) 122, 292, 478
BceAI ACGGC 1 cut(s) 545
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BfaI CTAG 3 cut(s) 83, 530, 658
BfmI CTRYAG 1 cut(s) 567
BfrI CTTAAG 1 cut(s) 91
BglII AGATCT 1 cut(s) 95
BlpI GCTNAGC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 1 cut(s) 245
BmsI GCATC 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 561
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse1I ACTGG 2 cut(s) 247, 403
BseCI ATCGAT 1 cut(s) 548
BseDI CCNNGG 1 cut(s) 561
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 2 cut(s) 247, 403
BsgI GTGCAG 1 cut(s) 655
BshFI GGCC 3 cut(s) 162, 302, 402
BshVI ATCGAT 1 cut(s) 548
BsiHKAI GWGCWC 2 cut(s) 82, 231
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 3 cut(s) 162, 302, 402
Bsp1286I GDGCHC 2 cut(s) 82, 231
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 2 cut(s) 95, 577
Bsp1720I GCTNAGC 1 cut(s) 121
BspACI CCGC 3 cut(s) 118, 352, 461
BspANI GGCC 3 cut(s) 162, 302, 402
BspCNI CTCAG 1 cut(s) 134
BspDI ATCGAT 1 cut(s) 548
BspLI GGNNCC 1 cut(s) 245
BspQI GCTCTTC 1 cut(s) 70
BspTI CTTAAG 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 120
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 247, 403
BssECI CCNNGG 1 cut(s) 561
BssMI GATC 2 cut(s) 95, 577
Bst4CI ACNGT 3 cut(s) 56, 541, 571
Bst6I CTCTTC 1 cut(s) 70
BstAFI CTTAAG 1 cut(s) 91
BstAUI TGTACA 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 121, 453
BstDSI CCRYGG 1 cut(s) 561
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 2 cut(s) 98, 580
BstMBI GATC 2 cut(s) 95, 577
BstMWI GCNNNNNNNGC 2 cut(s) 131, 609
BstNSI RCATGY 1 cut(s) 336
BstSFI CTRYAG 1 cut(s) 567
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
Bsu15I ATCGAT 1 cut(s) 548
BsuRI GGCC 3 cut(s) 162, 302, 402
BsuTUI ATCGAT 1 cut(s) 548
BtgI CCRYGG 1 cut(s) 561
BtgZI GCGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 3 cut(s) 61, 168, 573
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 548
CseI GACGC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 499
CviAII CATG 2 cut(s) 333, 619
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 2 cut(s) 121, 453
DpnI GATC 2 cut(s) 97, 579
DpnII GATC 2 cut(s) 95, 577
DraIII CACNNNGTG 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 113
EcoT38I GRGCYC 1 cut(s) 82
FaeI CATG 2 cut(s) 336, 622
FaiI YATR 8 cut(s) 206, 257, 279, 334, 386, 426, 609, 620
FaqI GGGAC 1 cut(s) 321
FatI CATG 2 cut(s) 332, 618
FauI CCCGC 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 82
FspBI CTAG 3 cut(s) 83, 530, 658
GlaI GCGC 1 cut(s) 47
HaeIII GGCC 3 cut(s) 162, 302, 402
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 48
Hin1II CATG 2 cut(s) 336, 622
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HincII GTYRAC 2 cut(s) 143, 641
HindII GTYRAC 2 cut(s) 143, 641
HinfI GANTC 1 cut(s) 37
HphI GGTGA 2 cut(s) 77, 256
Hpy166II GTNNAC 3 cut(s) 143, 217, 641
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 3 cut(s) 83, 214, 520
Hpy8I GTNNAC 3 cut(s) 143, 217, 641
HpyAV CCTTC 2 cut(s) 190, 517
HpyCH4III ACNGT 3 cut(s) 56, 541, 571
HpyCH4V TGCA 3 cut(s) 134, 332, 636
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 609
HpyF3I CTNAG 2 cut(s) 121, 453
Hsp92II CATG 2 cut(s) 336, 622
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 95, 577
LguI GCTCTTC 1 cut(s) 70
LpnPI CCDG 6 cut(s) 48, 177, 260, 274, 342, 384
LweI GCATC 1 cut(s) 49
MaeI CTAG 3 cut(s) 83, 530, 658
MaeIII GTNAC 2 cut(s) 463, 571
MalI GATC 2 cut(s) 97, 579
MbiI CCGCTC 1 cut(s) 120
MboI GATC 2 cut(s) 95, 577
MboII GAAGA 3 cut(s) 46, 87, 98
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 2 cut(s) 82, 231
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 9 cut(s) 113, 184, 192, 207, 221, 474, 488, 585, 646
MluNI TGGCCA 1 cut(s) 302
MnlI CCTC 4 cut(s) 12, 65, 335, 349
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 3 cut(s) 5, 92, 108
MslI CAYNNNNRTG 1 cut(s) 617
Msp20I TGGCCA 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 91
MunI CAATTG 1 cut(s) 474
MwoI GCNNNNNNNGC 2 cut(s) 131, 609
NdeII GATC 2 cut(s) 95, 577
NlaIII CATG 2 cut(s) 336, 622
NlaIV GGNNCC 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 571
NspI RCATGY 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 70
PcsI WCGNNNNNNNCGW 1 cut(s) 545
PfeI GAWTC 1 cut(s) 37
PflMI CCANNNNNTGG 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 245
PspPI GGNCC 1 cut(s) 161
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 1 cut(s) 95
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 1 cut(s) 617
SacI GAGCTC 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 3 cut(s) 5, 92, 108
Sau3AI GATC 2 cut(s) 95, 577
Sau96I GGNCC 1 cut(s) 161
SduI GDGCHC 2 cut(s) 82, 231
SetI ASST 6 cut(s) 82, 182, 269, 290, 419, 528
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 567
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 2 cut(s) 41, 91
SmoI CTYRAG 2 cut(s) 41, 91
Sse9I AATT 9 cut(s) 113, 184, 192, 207, 221, 474, 488, 585, 646
SsiI CCGC 3 cut(s) 118, 352, 461
SspMI CTAG 3 cut(s) 83, 530, 658
SstI GAGCTC 1 cut(s) 82
TaaI ACNGT 3 cut(s) 56, 541, 571
TaqI TCGA 3 cut(s) 347, 521, 548
TasI AATT 9 cut(s) 113, 184, 192, 207, 221, 474, 488, 585, 646
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 3 cut(s) 5, 92, 108
Tru9I TTAA 3 cut(s) 5, 92, 108
TscAI CASTG 3 cut(s) 61, 175, 580
TseFI GTSAC 1 cut(s) 571
Tsp45I GTSAC 1 cut(s) 571
TspDTI ATGAA 2 cut(s) 126, 579
TspRI CASTG 3 cut(s) 61, 175, 580
Van91I CCANNNNNTGG 1 cut(s) 280
Vha464I CTTAAG 1 cut(s) 91
XapI RAATTY 3 cut(s) 113, 488, 646
XbaI TCTAGA 1 cut(s) 82
XceI RCATGY 1 cut(s) 336
XspI CTAG 3 cut(s) 83, 530, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.