pycom13g28370

Protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009654
Physical Location & Seq
Forward (+)
24241455 .. 24245379
3925 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2022 bp
ATGGAGTTTGACTCAAGAGACGAAGCTAGAGAATTTTATGTTGCATATGGTAGACGCTCCGGTTTCACCGTATGGATACACCACAATCGTCGTTCACGAGTAAATAATCAGGTTATTGGTCAAGATTTTGTTTGCTTGGAAGAAGGCTTTCGGGCACAAAAGTATGTGCACAGAAAAGACAGAGTTCTTCCTCCACCCCCAGTAACCTGCGAGGGCTGTCAAGCTATGATAAGGCTAGCTCAAAGAGAGGGACTGAAGTGGGTAGTCACAAAATTTGTTAAAGAGCATAATCATAAACTAATGAGTCCCAGCAAGGTTCCATGGCGGGAATCAGGAATGCATTTAGCAAGTGAGGATGAGAAGGATAAAAGAATCCGAGAGCTGTCCCTGGAGTTGTACAACGAGAGGCAAAAATGGAAGCAACGTTGTGCTGCATGTGAAGAACAGTTAAATATGATCTTGAAAGATTTGGAAAGACACACAGAGCACATGTCAAAAAAAGTTTCAGATACAGTTCAGAGCATAAAAGAAATTGAGGAGGAACAATCAGAGGACTCGGACAGCGGATGGACAACCCATTTTATCAGTTTTAGGAGCCATGTAGCTACCAATTCACCTATGGTGCCGTTCGAAGTTGGCGCAGAGGATAAATTAAGTCCACAGAACGAAAGTCTTTCAGGAAAAAGGTGGCAGCTGATGTTTGGTGTCTGGATCAAGGTGCTCATCCTCATTGAAGGCTTTATGATGTTCCGGGTCATTCTCTTGTTGAATTGTAATGTGATTTTGTTTTCCTCTCATATCAAATTTGAAATTGATTGGGAAGTATTATTGGCACTCCAAAAATCTCATTCTACACTCCTCACAAGTGTATTTTTCTTTCCCAATATAGAAAGTTTGGAGTGTAGAATGAGATTTTTAGAGTTGGTAAGCATTTTGTCCAAGCCTCTTGATAAGATTTTTACTACTTGCAAAAGTAGGGATAAAAACACTTCAAAATACTTGCAAGAGAACAAGGTAATCGTAAACAATTTATGTGAAATCAAATCTTATTTAAATAAAAAGGATTTAAGAAATCGACTAAAAAACAATTTAAAGAAAATCAATTTGAAAGGAAACCACATTAACAATCATTTGTTAGGTTTTCCTAAAGTATGTTCTACTTGGACCTCCAACCTAAAACGTATATCAAACATGCAACCCGTTTGGACGTTCGGGTTGAATGTGAACACGAAGGGCTTATTACTTTATGAAAACCCTTTAAGAAACCATGCAACTCTTAAGACGTGGTCGCTAATTTTAGGGAACCTTCCGACCAAAATTGCATCAAATTACTTTTCTAAACATCCTCATGGCCATGAATCACTAAGACACTTAGATAGCTTAAACATGGTGATTAATAATTCAAAACATATTCTTGCTACGGCTCGTGGCCTCGCCCTAGCAAAAGAGACTAGTTATCACAATCATAAAGAAAAAAATTATAAAGAACACCTTGTAAATAAACTTGAATCGTCAAAACCTTATTTATACTACTACAAAATAAAATCCTTCCTAGAAAAGGAAATATATTCCCATTCTAACTTTGGGAAATTTGGGCAGCCGCAAAGAATCCCATGTTTTTTACCCTTCAAAGCTCCAAATCAGACCTTAAAGGACTCACGAACATCCTTCAATTGGAATCACTCCAAAATTCATCCGTTTGACCACTTTTTGCTCATGAGGAAATCACATCAAAAGAAAGTTCACTTGATCAAACTCTTGTACTGCAAGTTAGAGGATCAAATGGCAAACATCTTCACCAAGGCACTGCCTAAAGCAAGATTTGTGTACTTGAGAGGTCTACTTTGGAGTCGAGACCATAAATCATTTAGAGGGGAGTCAAGGAAGACTAAAAGGGAGCAACGACTAACACATGTAAGGCTGAGATTAGTGTGTGTTGCTGATAGGAGCATATTCATGCGACTTAAATGGCTTGTTCTCGTGCATTTACGTTATGTTTCTTTAGTTATTTTAGTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

674

Amino Acids

79.27

Weight (kDa)

9.77

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 11 - 101 3.1e-21 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1482
Acc36I ACCTGC 1 cut(s) 215
AccB1I GGYRCC 1 cut(s) 622
AccI GTMKAC 2 cut(s) 52, 1840
AciI CCGC 3 cut(s) 325, 564, 1601
AclI AACGTT 1 cut(s) 424
AclWI GGATC 2 cut(s) 719, 1785
AcoI YGGCCR 1 cut(s) 1351
AcsI RAATTY 5 cut(s) 32, 272, 803, 1589, 1689
AcuI CTGAAG 1 cut(s) 275
AfaI GTAC 4 cut(s) 398, 1763, 1829, 2016
AfiI CCNNNNNNNGG 2 cut(s) 1558, 1674
AflII CTTAAG 1 cut(s) 1277
AflIII ACRYGT 2 cut(s) 489, 1912
AhlI ACTAGT 1 cut(s) 1451
AjiI CACGTC 1 cut(s) 1284
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 8 cut(s) 26, 224, 239, 382, 605, 694, 1380, 1634
AluI AGCT 8 cut(s) 26, 224, 239, 382, 605, 694, 1380, 1634
Alw21I GWGCWC 3 cut(s) 171, 489, 723
Alw26I GTCTC 3 cut(s) 12, 1442, 1848
Alw44I GTGCAC 1 cut(s) 167
AlwI GGATC 2 cut(s) 719, 1785
AoxI GGCC 2 cut(s) 1351, 1429
ApaLI GTGCAC 1 cut(s) 167
ApeKI GCWGC 3 cut(s) 431, 691, 1597
ApoI RAATTY 5 cut(s) 32, 272, 803, 1589, 1689
AseI ATTAAT 1 cut(s) 1395
Asp700I GAANNNNTTC 1 cut(s) 147
AspLEI GCGC 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 1164
AsuC2I CCSGG 1 cut(s) 752
AsuHPI GGTGA 4 cut(s) 58, 606, 1402, 1789
AsuII TTCGAA 1 cut(s) 630
AsuNHI GCTAGC 1 cut(s) 235
AvaII GGWCC 1 cut(s) 1164
BaeGI GKGCMC 2 cut(s) 157, 171
BalI TGGCCA 1 cut(s) 1353
BanI GGYRCC 1 cut(s) 622
BauI CACGAG 3 cut(s) 96, 1425, 1979
BbsI GAAGAC 1 cut(s) 1892
Bbv12I GWGCWC 3 cut(s) 171, 489, 723
BbvI GCAGC 3 cut(s) 418, 703, 1609
BccI CCATC 1 cut(s) 561
BceAI ACGGC 2 cut(s) 610, 1437
BciT130I CCWGG 1 cut(s) 389
BciVI GTATCC 1 cut(s) 69
BclI TGATCA 1 cut(s) 1749
BcnI CCSGG 1 cut(s) 752
BcoDI GTCTC 3 cut(s) 12, 1442, 1848
BcuI ACTAGT 1 cut(s) 1451
BfaI CTAG 5 cut(s) 27, 236, 1439, 1452, 1553
BfrI CTTAAG 1 cut(s) 1277
BfuAI ACCTGC 1 cut(s) 215
BfuI GTATCC 1 cut(s) 69
BisI GCNGC 4 cut(s) 432, 692, 1598, 1601
BlsI GCNGC 4 cut(s) 433, 693, 1599, 1602
BmcAI AGTACT 1 cut(s) 2016
Bme1390I CCNGG 2 cut(s) 389, 752
Bme18I GGWCC 1 cut(s) 1164
BmgBI CACGTC 1 cut(s) 1284
BmgT120I GGNCC 1 cut(s) 1164
BmiI GGNNCC 4 cut(s) 318, 596, 624, 1304
BmrFI CCNGG 2 cut(s) 389, 752
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 1331
BmtI GCTAGC 1 cut(s) 239
BmuI ACTGGG 1 cut(s) 194
BpiI GAAGAC 1 cut(s) 1892
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 410
Bpu14I TTCGAA 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 1852
BpuMI CCSGG 1 cut(s) 752
BsaI GGTCTC 1 cut(s) 1848
BsaJI CCNNGG 3 cut(s) 320, 387, 1800
BsaWI WCCGGW 1 cut(s) 59
Bsc4I CCNNNNNNNGG 2 cut(s) 1558, 1674
Bse1I ACTGG 1 cut(s) 200
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 3 cut(s) 320, 387, 1800
BseGI GGATG 6 cut(s) 361, 572, 723, 1342, 1664, 1693
BseLI CCNNNNNNNGG 2 cut(s) 1558, 1674
BseMII CTCAG 1 cut(s) 1913
BseNI ACTGG 1 cut(s) 200
BseRI GAGGAG 2 cut(s) 551, 848
BseSI GKGCMC 2 cut(s) 157, 171
BseXI GCAGC 3 cut(s) 418, 703, 1609
BseYI CCCAGC 1 cut(s) 308
BshFI GGCC 2 cut(s) 1353, 1431
BshNI GGYRCC 1 cut(s) 622
BsiHKAI GWGCWC 3 cut(s) 171, 489, 723
BsiSI CCGG 2 cut(s) 60, 751
BslFI GGGAC 3 cut(s) 264, 291, 370
BslI CCNNNNNNNGG 2 cut(s) 1558, 1674
BsmAI GTCTC 3 cut(s) 12, 1442, 1848
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 3 cut(s) 264, 291, 370
BsmI GAATGC 1 cut(s) 342
BsnI GGCC 2 cut(s) 1353, 1431
Bso31I GGTCTC 1 cut(s) 1848
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 4 cut(s) 157, 171, 489, 723
Bsp1407I TGTACA 1 cut(s) 396
Bsp143I GATC 4 cut(s) 456, 711, 1749, 1777
Bsp19I CCATGG 1 cut(s) 320
BspACI CCGC 3 cut(s) 325, 564, 1601
BspANI GGCC 2 cut(s) 1353, 1431
BspCNI CTCAG 1 cut(s) 1914
BspHI TCATGA 1 cut(s) 1716
BspLI GGNNCC 4 cut(s) 318, 596, 624, 1304
BspMI ACCTGC 1 cut(s) 215
BspOI GCTAGC 1 cut(s) 239
BspPI GGATC 2 cut(s) 719, 1785
BspT104I TTCGAA 1 cut(s) 630
BspT107I GGYRCC 1 cut(s) 622
BspTI CTTAAG 1 cut(s) 1277
BspTNI GGTCTC 1 cut(s) 1848
BsrGI TGTACA 1 cut(s) 396
BsrI ACTGG 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 320, 387, 1800
BssMI GATC 4 cut(s) 456, 711, 1749, 1777
BssSI CACGAG 3 cut(s) 96, 1425, 1979
BssT1I CCWWGG 2 cut(s) 320, 1800
Bst2BI CACGAG 3 cut(s) 96, 1425, 1979
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 3 cut(s) 70, 447, 514
BstAFI CTTAAG 1 cut(s) 1277
BstAUI TGTACA 1 cut(s) 396
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 3 cut(s) 1364, 1372, 1922
BstDSI CCRYGG 1 cut(s) 320
BstENI CCTNNNNNAGG 1 cut(s) 1556
BstF5I GGATG 6 cut(s) 361, 572, 723, 1342, 1664, 1693
BstHHI GCGC 1 cut(s) 641
BstKTI GATC 4 cut(s) 459, 714, 1752, 1780
BstMAI GTCTC 3 cut(s) 12, 1442, 1848
BstMBI GATC 4 cut(s) 456, 711, 1749, 1777
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 4 cut(s) 438, 493, 1195, 1916
BstSCI CCNGG 2 cut(s) 387, 750
BstSLI GKGCMC 2 cut(s) 157, 171
BstV1I GCAGC 3 cut(s) 418, 703, 1609
BstV2I GAAGAC 1 cut(s) 1892
BsuI GTATCC 1 cut(s) 69
BsuRI GGCC 2 cut(s) 1353, 1431
BtgI CCRYGG 1 cut(s) 320
BtrI CACGTC 1 cut(s) 1284
BtsCI GGATG 6 cut(s) 361, 572, 723, 1342, 1664, 1693
BtsI GCAGTG 1 cut(s) 1805
BtsIMutI CAGTG 1 cut(s) 1805
BveI ACCTGC 1 cut(s) 215
Cac8I GCNNGC 1 cut(s) 237
CciI TCATGA 1 cut(s) 1716
CfoI GCGC 1 cut(s) 641
Cfr13I GGNCC 1 cut(s) 1164
CseI GACGC 1 cut(s) 63
Csp6I GTAC 4 cut(s) 397, 1762, 1828, 2015
CviQI GTAC 4 cut(s) 397, 1762, 1828, 2015
DdeI CTNAG 3 cut(s) 1364, 1372, 1922
DpnI GATC 4 cut(s) 458, 713, 1751, 1779
DpnII GATC 4 cut(s) 456, 711, 1749, 1777
DraI TTTAAA 2 cut(s) 1053, 1092
EaeI YGGCCR 1 cut(s) 1351
Eco130I CCWWGG 2 cut(s) 320, 1800
Eco31I GGTCTC 1 cut(s) 1848
Eco47I GGWCC 1 cut(s) 1164
Eco57I CTGAAG 1 cut(s) 275
EcoNI CCTNNNNNAGG 1 cut(s) 1556
EcoRII CCWGG 1 cut(s) 387
EcoT14I CCWWGG 2 cut(s) 320, 1800
EcoT22I ATGCAT 1 cut(s) 342
ErhI CCWWGG 2 cut(s) 320, 1800
Esp3I CGTCTC 1 cut(s) 12
FalI AAGNNNNNCTT 2 cut(s) 1476, 1508
FaqI GGGAC 3 cut(s) 264, 291, 370
FauI CCCGC 1 cut(s) 318
FauNDI CATATG 1 cut(s) 46
FbaI TGATCA 1 cut(s) 1749
FblI GTMKAC 2 cut(s) 52, 1840
Fnu4HI GCNGC 4 cut(s) 432, 692, 1598, 1601
FokI GGATG 6 cut(s) 368, 579, 710, 1329, 1651, 1680
Fsp4HI GCNGC 4 cut(s) 432, 692, 1598, 1601
FspBI CTAG 5 cut(s) 27, 236, 1439, 1452, 1553
GlaI GCGC 1 cut(s) 640
GluI GCNGC 4 cut(s) 432, 692, 1598, 1601
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 1 cut(s) 410
HaeIII GGCC 2 cut(s) 1353, 1431
HapII CCGG 2 cut(s) 60, 751
HgaI GACGC 1 cut(s) 63
HhaI GCGC 1 cut(s) 641
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HpaII CCGG 2 cut(s) 60, 751
HphI GGTGA 4 cut(s) 58, 606, 1402, 1789
Hpy166II GTNNAC 9 cut(s) 53, 95, 169, 659, 1024, 1225, 1744, 1828, 1841
Hpy188I TCNGA 7 cut(s) 377, 508, 519, 550, 559, 1311, 1644
Hpy8I GTNNAC 9 cut(s) 53, 95, 169, 659, 1024, 1225, 1744, 1828, 1841
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 8 cut(s) 137, 355, 728, 1225, 1316, 1558, 1636, 1678
HpyCH4III ACNGT 3 cut(s) 70, 447, 514
HpyCH4IV ACGT 5 cut(s) 424, 1180, 1208, 1283, 1990
HpyF3I CTNAG 3 cut(s) 1364, 1372, 1922
HpySE526I ACGT 5 cut(s) 424, 1180, 1208, 1283, 1990
HspAI GCGC 1 cut(s) 639
Ksp22I TGATCA 1 cut(s) 1749
Kzo9I GATC 4 cut(s) 456, 711, 1749, 1777
LmnI GCTCC 5 cut(s) 62, 594, 1639, 1897, 1947
Lsp1109I GCAGC 3 cut(s) 418, 703, 1609
LweI GCATC 1 cut(s) 1331
MaeI CTAG 5 cut(s) 27, 236, 1439, 1452, 1553
MaeII ACGT 5 cut(s) 424, 1180, 1208, 1283, 1990
MaeIII GTNAC 2 cut(s) 202, 265
MalI GATC 4 cut(s) 458, 713, 1751, 1779
MboI GATC 4 cut(s) 456, 711, 1749, 1777
MboII GAAGA 5 cut(s) 152, 179, 452, 1786, 1897
MfeI CAATTG 1 cut(s) 1672
MhlI GDGCHC 4 cut(s) 157, 171, 489, 723
MlsI TGGCCA 1 cut(s) 1353
MluNI TGGCCA 1 cut(s) 1353
MlyI GAGTC 6 cut(s) 5, 313, 548, 1649, 1858, 1886
MmeI TCCRAC 2 cut(s) 1194, 1334
Mox20I TGGCCA 1 cut(s) 1353
Mph1103I ATGCAT 1 cut(s) 342
MroXI GAANNNNTTC 1 cut(s) 147
MscI TGGCCA 1 cut(s) 1353
MslI CAYNNNNRTG 3 cut(s) 1347, 1353, 1955
Msp20I TGGCCA 1 cut(s) 1353
MspA1I CMGCKG 2 cut(s) 564, 694
MspCI CTTAAG 1 cut(s) 1277
MspI CCGG 2 cut(s) 60, 751
MspR9I CCNGG 2 cut(s) 389, 752
MunI CAATTG 1 cut(s) 1672
Mva1269I GAATGC 1 cut(s) 342
MvaI CCWGG 1 cut(s) 389
NciI CCSGG 1 cut(s) 752
NcoI CCATGG 1 cut(s) 320
NdeI CATATG 1 cut(s) 46
NdeII GATC 4 cut(s) 456, 711, 1749, 1777
NheI GCTAGC 1 cut(s) 235
NlaIV GGNNCC 4 cut(s) 318, 596, 624, 1304
NmuCI GTSAC 1 cut(s) 265
NsiI ATGCAT 1 cut(s) 342
NspI RCATGY 4 cut(s) 438, 493, 1195, 1916
NspV TTCGAA 1 cut(s) 630
PagI TCATGA 1 cut(s) 1716
PciI ACATGT 2 cut(s) 489, 1912
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PctI GAATGC 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 6 cut(s) 329, 372, 1358, 1508, 1608, 1678
PflFI GACNNNGTC 1 cut(s) 1285
PkrI GCNGC 4 cut(s) 433, 693, 1599, 1602
PleI GAGTC 6 cut(s) 5, 312, 548, 1649, 1857, 1885
PpsI GAGTC 6 cut(s) 5, 312, 548, 1649, 1857, 1885
PscI ACATGT 2 cut(s) 489, 1912
PshBI ATTAAT 1 cut(s) 1395
PsiI TTATAA 1 cut(s) 1482
Psp1406I AACGTT 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 387
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 4 cut(s) 318, 596, 624, 1304
PspPI GGNCC 1 cut(s) 1164
PsyI GACNNNGTC 1 cut(s) 1285
PvuII CAGCTG 1 cut(s) 694
RsaI GTAC 4 cut(s) 398, 1763, 1829, 2016
RsaNI GTAC 4 cut(s) 397, 1762, 1828, 2015
RseI CAYNNNNRTG 3 cut(s) 1347, 1353, 1955
SatI GCNGC 4 cut(s) 432, 692, 1598, 1601
Sau3AI GATC 4 cut(s) 456, 711, 1749, 1777
Sau96I GGNCC 1 cut(s) 1164
ScaI AGTACT 1 cut(s) 2016
SchI GAGTC 6 cut(s) 5, 313, 548, 1649, 1858, 1886
ScrFI CCNGG 2 cut(s) 389, 752
SduI GDGCHC 4 cut(s) 157, 171, 489, 723
SfaNI GCATC 1 cut(s) 1331
SfuI TTCGAA 1 cut(s) 630
SinI GGWCC 1 cut(s) 1164
SmiI ATTTAAAT 1 cut(s) 1053
SmiMI CAYNNNNRTG 3 cut(s) 1347, 1353, 1955
SmlI CTYRAG 3 cut(s) 13, 1277, 1831
SmoI CTYRAG 3 cut(s) 13, 1277, 1831
SpeI ACTAGT 1 cut(s) 1451
SsiI CCGC 3 cut(s) 325, 564, 1601
SspMI CTAG 5 cut(s) 27, 236, 1439, 1452, 1553
StyD4I CCNGG 2 cut(s) 387, 750
StyI CCWWGG 2 cut(s) 320, 1800
SwaI ATTTAAAT 1 cut(s) 1053
TaaI ACNGT 3 cut(s) 70, 447, 514
TaiI ACGT 5 cut(s) 427, 1183, 1211, 1286, 1993
TaqI TCGA 3 cut(s) 630, 1075, 1852
TatI WGTACW 4 cut(s) 396, 1761, 1827, 2014
TauI GCSGC 1 cut(s) 1603
TfiI GAWTC 6 cut(s) 329, 372, 1358, 1508, 1608, 1678
TscAI CASTG 1 cut(s) 1812
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 3 cut(s) 431, 691, 1597
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 4 cut(s) 1263, 1371, 1682, 1945
TspGWI ACGGA 1 cut(s) 1686
TspRI CASTG 1 cut(s) 1812
Tth111I GACNNNGTC 1 cut(s) 1285
Vha464I CTTAAG 1 cut(s) 1277
VneI GTGCAC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 1164
VspI ATTAAT 1 cut(s) 1395
XagI CCTNNNNNAGG 1 cut(s) 1556
XapI RAATTY 5 cut(s) 32, 272, 803, 1589, 1689
XceI RCATGY 4 cut(s) 438, 493, 1195, 1916
XcmI CCANNNNNNNNNTGG 2 cut(s) 616, 1580
XmiI GTMKAC 2 cut(s) 52, 1840
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 5 cut(s) 27, 236, 1439, 1452, 1553
ZrmI AGTACT 1 cut(s) 2016
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.