pycom14g01150

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005474
Physical Location & Seq
Forward (+)
808640 .. 810621
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGAAAGGCCAAAGCAGTTACGCTCCACCTGCTTACATTCCGTTGGGAATGTCAGATTCTGATTCAGCGATTGTTTTGCGAAATGAAGACCCGCCCAATCAGCAGAACAGCATTGATGGGCCCAAACAATGGTCTTCTGGAATCTGCGCATGCTGTGACGATATGCAGAGCTGTTGTGTAGGTCTATTTTGTCCATGCTTTCTGTTTGGAAAGAATGCAGAATTTCTGGGTTCTGGGACATTATTAGGATCATGCATGACTCATTTTATTTTGTGGGCACTTGTTAATACCGCCTGCTGCTTATTGACTGAGGGCCTGTTTTTGGGCATACCGGGATGCTTTGTTGCATGTTATGCTTGTGGCTACCGCAGTGCCCTAAGAACAAAGTATAATCTTCAGGAAGCACCATGTGGAGACTTCGTCACTCATTTTTTCTGCCATCTGTGTGCACTTTGTCAAGAGTACCGGGAGATCCGTGAAAAGTCTGATTCCGGCGCTCCTGACCTGAGTCTGGCTGTAGTTACAGCCCCACCAGTCCAGACAATGGAACCAGGTCTCCAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

189

Amino Acids

20.32

Weight (kDa)

4.9

Isoelectric Point (pI)

59.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 44 - 157 4.4e-29 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 37
Acc16I TGCGCA 1 cut(s) 148
Acc36I ACCTGC 1 cut(s) 37
AccB7I CCANNNNNTGG 2 cut(s) 129, 544
AciI CCGC 3 cut(s) 92, 291, 367
AclWI GGATC 2 cut(s) 256, 466
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 1 cut(s) 380
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 4 cut(s) 129, 322, 511, 544
AjnI CCWGG 1 cut(s) 550
AloI GAACNNNNNNTCC 1 cut(s) 540
AluBI AGCT 1 cut(s) 171
AluI AGCT 1 cut(s) 171
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 2 cut(s) 408, 560
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 2 cut(s) 256, 466
AlwNI CAGNNNCTG 1 cut(s) 59
AoxI GGCC 3 cut(s) 7, 119, 313
ApaI GGGCCC 1 cut(s) 123
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 297
ApoI RAATTY 1 cut(s) 221
AspLEI GCGC 2 cut(s) 149, 497
AspS9I GGNCC 3 cut(s) 119, 120, 313
AsuC2I CCSGG 2 cut(s) 333, 467
BaeGI GKGCMC 4 cut(s) 123, 280, 376, 451
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 93, 126
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 1 cut(s) 284
BccI CCATC 2 cut(s) 110, 447
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 1 cut(s) 552
BcnI CCSGG 2 cut(s) 333, 467
BcoDI GTCTC 2 cut(s) 408, 560
BfmI CTRYAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 498
BfuAI ACCTGC 1 cut(s) 37
BisI GCNGC 1 cut(s) 298
BlsI GCNGC 1 cut(s) 299
Bme1390I CCNGG 3 cut(s) 333, 467, 552
BmgT120I GGNCC 3 cut(s) 119, 120, 313
BmiI GGNNCC 2 cut(s) 121, 549
BmrFI CCNGG 3 cut(s) 333, 467, 552
BmsI GCATC 1 cut(s) 326
BoxI GACNNNNGTC 1 cut(s) 507
BpiI GAAGAC 2 cut(s) 93, 126
BpuMI CCSGG 2 cut(s) 333, 467
BsaI GGTCTC 1 cut(s) 560
BsaXI ACNNNNNCTCC 3 cut(s) 405, 435, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 129, 322, 511, 544
Bse1I ACTGG 1 cut(s) 533
BseBI CCWGG 1 cut(s) 552
BseGI GGATG 1 cut(s) 341
BseLI CCNNNNNNNGG 4 cut(s) 129, 322, 511, 544
BseMII CTCAG 2 cut(s) 300, 497
BseNI ACTGG 1 cut(s) 533
BseSI GKGCMC 4 cut(s) 123, 280, 376, 451
BseXI GCAGC 1 cut(s) 284
BshFI GGCC 3 cut(s) 9, 121, 315
BsiHKAI GWGCWC 1 cut(s) 451
BsiSI CCGG 3 cut(s) 332, 466, 492
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 4 cut(s) 129, 322, 511, 544
BsmAI GTCTC 2 cut(s) 408, 560
BsmFI GGGAC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 3 cut(s) 9, 121, 315
Bso31I GGTCTC 1 cut(s) 560
Bsp120I GGGCCC 1 cut(s) 119
Bsp1286I GDGCHC 4 cut(s) 123, 280, 376, 451
Bsp143I GATC 2 cut(s) 248, 471
BspACI CCGC 3 cut(s) 92, 291, 367
BspANI GGCC 3 cut(s) 9, 121, 315
BspCNI CTCAG 2 cut(s) 301, 498
BspLI GGNNCC 2 cut(s) 121, 549
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 2 cut(s) 256, 466
BspTNI GGTCTC 1 cut(s) 560
BsrI ACTGG 1 cut(s) 533
BssMI GATC 2 cut(s) 248, 471
Bst2UI CCWGG 1 cut(s) 552
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 2 cut(s) 151, 295
BstDEI CTNAG 3 cut(s) 309, 377, 506
BstF5I GGATG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 2 cut(s) 149, 497
BstKTI GATC 2 cut(s) 251, 474
BstMAI GTCTC 2 cut(s) 408, 560
BstMBI GATC 2 cut(s) 248, 471
BstMWI GCNNNNNNNGC 3 cut(s) 29, 100, 353
BstNI CCWGG 1 cut(s) 552
BstNSI RCATGY 2 cut(s) 153, 351
BstPAI GACNNNNGTC 1 cut(s) 507
BstSCI CCNGG 3 cut(s) 331, 465, 550
BstSFI CTRYAG 1 cut(s) 516
BstSLI GKGCMC 4 cut(s) 123, 280, 376, 451
BstV1I GCAGC 1 cut(s) 284
BstV2I GAAGAC 2 cut(s) 93, 126
BstX2I RGATCY 1 cut(s) 471
BstYI RGATCY 1 cut(s) 471
BsuRI GGCC 3 cut(s) 9, 121, 315
BtsCI GGATG 1 cut(s) 341
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 376
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 2 cut(s) 151, 295
CaiI CAGNNNCTG 1 cut(s) 59
CfoI GCGC 2 cut(s) 149, 497
Cfr13I GGNCC 3 cut(s) 119, 120, 313
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 1 cut(s) 463
CviAII CATG 6 cut(s) 150, 195, 252, 256, 348, 408
CviJI RGCY 7 cut(s) 9, 121, 171, 315, 363, 515, 527
CviKI_1 RGCY 7 cut(s) 9, 121, 171, 315, 363, 515, 527
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 3 cut(s) 309, 377, 506
DpnI GATC 2 cut(s) 250, 473
DpnII GATC 2 cut(s) 248, 471
DraIII CACNNNGTG 1 cut(s) 410
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 560
Eco57I CTGAAG 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 550
EcoT22I ATGCAT 1 cut(s) 257
EcoT38I GRGCYC 1 cut(s) 123
FaeI CATG 6 cut(s) 153, 198, 255, 259, 351, 411
FaqI GGGAC 1 cut(s) 250
FatI CATG 6 cut(s) 149, 194, 251, 255, 347, 407
FauI CCCGC 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 298
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 298
FspI TGCGCA 1 cut(s) 148
GlaI GCGC 2 cut(s) 148, 496
GluI GCNGC 1 cut(s) 298
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 3 cut(s) 9, 121, 315
HapII CCGG 3 cut(s) 332, 466, 492
HhaI GCGC 2 cut(s) 149, 497
Hin1II CATG 6 cut(s) 153, 198, 255, 259, 351, 411
Hin6I GCGC 2 cut(s) 147, 495
HinP1I GCGC 2 cut(s) 147, 495
HinfI GANTC 6 cut(s) 56, 62, 141, 259, 488, 508
HpaII CCGG 3 cut(s) 332, 466, 492
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 3 cut(s) 55, 61, 487
Hpy188III TCNNGA 5 cut(s) 138, 398, 458, 500, 538
Hpy8I GTNNAC 1 cut(s) 449
HpyCH4V TGCA 5 cut(s) 166, 218, 255, 347, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 100, 353
HpyF3I CTNAG 3 cut(s) 309, 377, 506
Hsp92II CATG 6 cut(s) 153, 198, 255, 259, 351, 411
HspAI GCGC 2 cut(s) 147, 495
Kzo9I GATC 2 cut(s) 248, 471
LmnI GCTCC 2 cut(s) 28, 502
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 1 cut(s) 326
MabI ACCWGGT 1 cut(s) 550
MaeIII GTNAC 4 cut(s) 17, 155, 421, 520
MalI GATC 2 cut(s) 250, 473
MboI GATC 2 cut(s) 248, 471
MboII GAAGA 3 cut(s) 98, 126, 386
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 4 cut(s) 123, 280, 376, 451
MluCI AATT 1 cut(s) 221
MlyI GAGTC 2 cut(s) 253, 517
MnlI CCTC 1 cut(s) 304
Mph1103I ATGCAT 1 cut(s) 257
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 444
MspI CCGG 3 cut(s) 332, 466, 492
MspR9I CCNGG 3 cut(s) 333, 467, 552
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 552
MwoI GCNNNNNNNGC 3 cut(s) 29, 100, 353
NciI CCSGG 2 cut(s) 333, 467
NdeII GATC 2 cut(s) 248, 471
NlaIII CATG 6 cut(s) 153, 198, 255, 259, 351, 411
NlaIV GGNNCC 2 cut(s) 121, 549
NmuCI GTSAC 2 cut(s) 155, 421
NsbI TGCGCA 1 cut(s) 148
NsiI ATGCAT 1 cut(s) 257
NspI RCATGY 2 cut(s) 153, 351
PaeI GCATGC 1 cut(s) 153
PaqCI CACCTGC 1 cut(s) 37
PctI GAATGC 1 cut(s) 220
PfeI GAWTC 4 cut(s) 56, 62, 141, 488
PflFI GACNNNGTC 1 cut(s) 419
PflMI CCANNNNNTGG 2 cut(s) 129, 544
PkrI GCNGC 1 cut(s) 299
PleI GAGTC 2 cut(s) 253, 516
PpsI GAGTC 2 cut(s) 253, 516
PshAI GACNNNNGTC 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 550
PspGI CCWGG 1 cut(s) 550
PspN4I GGNNCC 2 cut(s) 121, 549
PspOMI GGGCCC 1 cut(s) 119
PspPI GGNCC 3 cut(s) 119, 120, 313
PstNI CAGNNNCTG 1 cut(s) 59
PsuI RGATCY 1 cut(s) 471
PsyI GACNNNGTC 1 cut(s) 419
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
RseI CAYNNNNRTG 1 cut(s) 444
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 298
Sau3AI GATC 2 cut(s) 248, 471
Sau96I GGNCC 3 cut(s) 119, 120, 313
SchI GAGTC 2 cut(s) 253, 517
ScrFI CCNGG 3 cut(s) 333, 467, 552
SduI GDGCHC 4 cut(s) 123, 280, 376, 451
SetI ASST 5 cut(s) 31, 173, 184, 507, 556
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 1 cut(s) 326
SfcI CTRYAG 1 cut(s) 516
SmiMI CAYNNNNRTG 1 cut(s) 444
SphI GCATGC 1 cut(s) 153
Sse9I AATT 1 cut(s) 221
SsiI CCGC 3 cut(s) 92, 291, 367
StyD4I CCNGG 3 cut(s) 331, 465, 550
TasI AATT 1 cut(s) 221
TfiI GAWTC 4 cut(s) 56, 62, 141, 488
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 2 cut(s) 155, 421
TseI GCWGC 1 cut(s) 297
Tsp45I GTSAC 2 cut(s) 155, 421
TspDTI ATGAA 2 cut(s) 17, 99
TspGWI ACGGA 2 cut(s) 30, 464
TspRI CASTG 1 cut(s) 376
Tth111I GACNNNGTC 1 cut(s) 419
Van91I CCANNNNNTGG 2 cut(s) 129, 544
VneI GTGCAC 1 cut(s) 447
XapI RAATTY 1 cut(s) 221
XceI RCATGY 2 cut(s) 153, 351
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.