pycom14g02160

Essential component of the cytosolic iron-sulfur (Fe S) protein assembly machinery. Required for the maturation of extramitochondrial Fe S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007416
Physical Location & Seq
Reverse (-)
1679115 .. 1683120
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1149 bp
ATGATTCATATTGTCCTCGTAGAGCAGTCAAAACACAGCATTTATACGGTCAAATACTCTTCCTCGCCGACTCCTCATAGCTCACAGCCTCATTTGGCCATGGAGTTAGCGAAAGAGCTCCGAGAGATTCAGACGCTACAGGGCCACACCGATAGGGTATGGAGCCTCGCCTGGAATCCGGCCACCGGTGTCGCCGGAATCCCCCTTTCCTTCGCTTCCTGCAGCGGCGATAAGACCGTTCGTATTTGGGAACAAAACCCCTCAACTTCTTCTTGGGATTGCAAGGCAGTTCTGGATGAAACACACACGAGAACCGTCCGATCGTGCGCTTGGTCACCATCTGGGAAATTATTGGCCACTGCAAGCTTCGATGCCACTACTGCAATTTGGGAAAATGTTGGGGGCGATTATGAATGTGTTGCCAGTCTAGAGGGTCATGAAAACGAAGTGAAAAGTGTGTGTTGGAATCCATCTGGGTCTATGCTTACGACTTGCGGTCGAGATAAAACTGTATGGGTATGGGAAGTGCTGCCCGGGAACGAGTTCGACTGTGTGTCAGTTTTGCAAGGCCATACACAAGATGTGAAGATGGTTCAGTGGCATCCCACTAGGAATCTTATATTTTCTTGTAGCTATGATAACACTGTCAAGATATGGGCTGATGATGGTGACGATGATGATTGGGCATGTGTTCAAACCTTAGGTGAAACTAACAATGGTCACTCTTCTACTGTCTGGGCTCTATCATTTAATGCCAGTGGAGACAAGATAGTTACCTGCAGTGATGATCTTACTTTAAAGATATGGGGATCAGACGATACAAAGATGTCTGCTGATGGTTTTGCACCTTGGCGACATCTTTGCACTCTTTCAGGTTATCATGACCGGACAATATTTTCAGTTCATTGGTCAAGGGACAATATCATAGCCAGTGGAGCTGCAGATGATGCAATAAGATTTTTCGTGGAGAACGATGAACAAGATGGTTTGGTTGATGGGCCTTCATATAAATTGCTTTTGAGGAAGGAAAATGCCCATGACATGGATATCAATTCGGTGCAATGGAGCCCTGGGGAGGATCAGTTTCTTGCTTCTGCAGCCGATGACGGGACGATTAAAATATGGGAGCTGACATCTGATAGCTGCTAA

Protein Analysis

383

Amino Acids

42.33

Weight (kDa)

4.73

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 36 - 133 2.6e-14 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 38 - 87 8e-07 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 38 - 177 3.1e-14 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 40 - 269 1.8e-11 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 41 - 176 6.1e-17 Prp19 WD40 domain
WD40 PF00400 42 - 83 2.2e-08 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 43 - 271 3.3e-24 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 53 - 220 6.3e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 70 - 178 1.8e-09 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 89 - 158 2.4e-07 WDHD1 first WD40 domain
WD40 PF00400 94 - 130 1.6e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 99 - 273 1.1e-16 CDC20/Fizzy WD40 domain
EIF3I PF24805 100 - 183 9.9e-06 EIF3I
Beta-prop_WDR3_1st PF25173 100 - 269 1.4e-26 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 101 - 275 7.7e-35 WDR5 beta-propeller domain
Beta-prop_EML PF23409 102 - 317 1.4e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 102 - 181 4.4e-18 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 136 - 272 3.1e-10 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 138 - 219 3.9e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 138 - 174 8.8e-11 WD domain, G-beta repeat
WD40_Prp19 PF24814 139 - 218 4e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 146 - 220 1.5e-17 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 147 - 230 5.1e-08 Aladin seven-bladed propeller
WD40_Prp19 PF24814 164 - 270 9e-16 Prp19 WD40 domain
WD40 PF00400 183 - 219 6.2e-10 WD domain, G-beta repeat
EIF3I PF24805 187 - 379 3.3e-08 EIF3I
WD40 PF00400 230 - 269 2.6e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 234 - 321 1.1e-08 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 237 - 321 3.4e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 238 - 382 1.8e-08 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 241 - 321 1.1e-08 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 245 - 379 7.7e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 292 - 380 5.1e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 292 - 382 1.5e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 294 - 381 4.7e-10 THOC3 beta-propeller domain
WD40 PF00400 343 - 375 2.8e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 785
AccB7I CCANNNNNTGG 1 cut(s) 1042
AciI CCGC 2 cut(s) 225, 495
AclWI GGATC 2 cut(s) 817, 1086
AcoI YGGCCR 3 cut(s) 96, 180, 354
AfiI CCNNNNNNNGG 4 cut(s) 185, 1042, 1075, 1107
AgeI ACCGGT 1 cut(s) 185
AgsI TTSAA 1 cut(s) 695
AhdI GACNNNNNGTC 2 cut(s) 495, 553
AjnI CCWGG 2 cut(s) 170, 1069
AluBI AGCT 7 cut(s) 81, 118, 366, 633, 938, 1129, 1143
AluI AGCT 7 cut(s) 81, 118, 366, 633, 938, 1129, 1143
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 756
AlwI GGATC 2 cut(s) 817, 1086
Ama87I CYCGRG 1 cut(s) 533
AoxI GGCC 6 cut(s) 96, 142, 180, 354, 568, 998
ApeKI GCWGC 5 cut(s) 222, 529, 938, 1097, 1143
AsiGI ACCGGT 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 542
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 2 cut(s) 142, 998
AsuC2I CCSGG 2 cut(s) 534, 535
AsuHPI GGTGA 3 cut(s) 327, 680, 716
AvaI CYCGRG 1 cut(s) 533
AxyI CCTNAGG 1 cut(s) 700
BalI TGGCCA 2 cut(s) 98, 356
BanII GRGCYC 3 cut(s) 120, 742, 1070
BauI CACGAG 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 5 cut(s) 234, 516, 925, 1109, 1130
BccI CCATC 7 cut(s) 346, 478, 583, 659, 830, 977, 989
BcgI CGANNNNNNTGC 2 cut(s) 843, 877
BciT130I CCWGG 2 cut(s) 172, 1071
BcnI CCSGG 2 cut(s) 534, 535
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 2 cut(s) 428, 609
BfmI CTRYAG 5 cut(s) 137, 220, 778, 939, 1095
BfuAI ACCTGC 1 cut(s) 785
BisI GCNGC 6 cut(s) 223, 226, 530, 939, 1098, 1144
BlsI GCNGC 6 cut(s) 224, 227, 531, 940, 1099, 1145
Bme1390I CCNGG 4 cut(s) 172, 534, 535, 1071
BmeRI GACNNNNNGTC 2 cut(s) 495, 553
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 2 cut(s) 142, 998
BmiI GGNNCC 2 cut(s) 164, 1067
BmrFI CCNGG 4 cut(s) 172, 534, 535, 1071
BmsI GCATC 3 cut(s) 361, 610, 937
BpuMI CCSGG 2 cut(s) 534, 535
BsaJI CCNNGG 5 cut(s) 99, 533, 848, 1069, 1070
BsaWI WCCGGW 2 cut(s) 185, 885
BsaXI ACNNNNNCTCC 2 cut(s) 1067, 1097
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 1042, 1075, 1107
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 3 cut(s) 423, 756, 930
Bse21I CCTNAGG 1 cut(s) 700
Bse3DI GCAATG 1 cut(s) 1067
BseBI CCWGG 2 cut(s) 172, 1071
BseDI CCNNGG 5 cut(s) 99, 533, 848, 1069, 1070
BseGI GGATG 2 cut(s) 301, 601
BseLI CCNNNNNNNGG 4 cut(s) 185, 1042, 1075, 1107
BseMI GCAATG 1 cut(s) 1067
BseNI ACTGG 3 cut(s) 423, 756, 930
BseRI GAGGAG 1 cut(s) 63
BseXI GCAGC 5 cut(s) 234, 516, 925, 1109, 1130
Bsh1285I CGRYCG 2 cut(s) 323, 499
BshFI GGCC 6 cut(s) 98, 144, 182, 356, 570, 1000
BshTI ACCGGT 1 cut(s) 185
BsiEI CGRYCG 2 cut(s) 323, 499
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 5 cut(s) 179, 186, 195, 534, 886
BslFI GGGAC 2 cut(s) 929, 1123
BslI CCNNNNNNNGG 4 cut(s) 185, 1042, 1075, 1107
BsmAI GTCTC 1 cut(s) 756
BsmFI GGGAC 2 cut(s) 929, 1123
BsnI GGCC 6 cut(s) 98, 144, 182, 356, 570, 1000
BsoBI CYCGRG 1 cut(s) 533
Bsp1286I GDGCHC 3 cut(s) 120, 742, 1070
Bsp143I GATC 4 cut(s) 320, 787, 809, 1078
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 2 cut(s) 225, 495
BspANI GGCC 6 cut(s) 98, 144, 182, 356, 570, 1000
BspHI TCATGA 2 cut(s) 436, 880
BspLI GGNNCC 2 cut(s) 164, 1067
BspMAI CTGCAG 4 cut(s) 224, 782, 943, 1099
BspMI ACCTGC 1 cut(s) 785
BspPI GGATC 2 cut(s) 817, 1086
BsrDI GCAATG 1 cut(s) 1067
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 3 cut(s) 423, 756, 930
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 5 cut(s) 99, 533, 848, 1069, 1070
BssMI GATC 4 cut(s) 320, 787, 809, 1078
BssSI CACGAG 1 cut(s) 307
BssT1I CCWWGG 2 cut(s) 99, 848
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 172, 1071
Bst4CI ACNGT 7 cut(s) 49, 238, 316, 511, 551, 646, 733
Bst6I CTCTTC 2 cut(s) 64, 730
BstAPI GCANNNNNTGC 1 cut(s) 947
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 1 cut(s) 700
BstDSI CCRYGG 1 cut(s) 99
BstEII GGTNACC 1 cut(s) 333
BstF5I GGATG 2 cut(s) 301, 601
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 4 cut(s) 323, 790, 812, 1081
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 4 cut(s) 320, 787, 809, 1078
BstMCI CGRYCG 2 cut(s) 323, 499
BstMWI GCNNNNNNNGC 4 cut(s) 380, 935, 947, 1097
BstNI CCWGG 2 cut(s) 172, 1071
BstNSI RCATGY 1 cut(s) 690
BstPI GGTNACC 1 cut(s) 333
BstSCI CCNGG 4 cut(s) 170, 532, 533, 1069
BstSFI CTRYAG 5 cut(s) 137, 220, 778, 939, 1095
BstV1I GCAGC 5 cut(s) 234, 516, 925, 1109, 1130
Bsu36I CCTNAGG 1 cut(s) 700
BsuRI GGCC 6 cut(s) 98, 144, 182, 356, 570, 1000
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 2 cut(s) 301, 601
BtsI GCAGTG 2 cut(s) 357, 787
BtsIMutI CAGTG 6 cut(s) 357, 602, 642, 763, 787, 937
BveI ACCTGC 1 cut(s) 785
Cac8I GCNNGC 1 cut(s) 364
CciI TCATGA 2 cut(s) 436, 880
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 142, 998
Cfr9I CCCGGG 1 cut(s) 533
CseI GACGC 1 cut(s) 142
CspAI ACCGGT 1 cut(s) 185
CviAII CATG 6 cut(s) 100, 437, 687, 881, 1037, 1042
DdeI CTNAG 1 cut(s) 700
DpnI GATC 4 cut(s) 322, 789, 811, 1080
DpnII GATC 4 cut(s) 320, 787, 809, 1078
DraI TTTAAA 1 cut(s) 798
DriI GACNNNNNGTC 2 cut(s) 495, 553
EaeI YGGCCR 3 cut(s) 96, 180, 354
Eam1104I CTCTTC 2 cut(s) 64, 730
Eam1105I GACNNNNNGTC 2 cut(s) 495, 553
EarI CTCTTC 2 cut(s) 64, 730
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 99, 848
Eco24I GRGCYC 3 cut(s) 120, 742, 1070
Eco32I GATATC 1 cut(s) 1048
Eco53kI GAGCTC 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 700
Eco88I CYCGRG 1 cut(s) 533
Eco91I GGTNACC 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 118
EcoO65I GGTNACC 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 170, 1069
EcoRV GATATC 1 cut(s) 1048
EcoT14I CCWWGG 2 cut(s) 99, 848
EcoT38I GRGCYC 3 cut(s) 120, 742, 1070
ErhI CCWWGG 2 cut(s) 99, 848
FaeI CATG 6 cut(s) 103, 440, 690, 884, 1040, 1045
FaqI GGGAC 2 cut(s) 929, 1123
FatI CATG 6 cut(s) 99, 436, 686, 880, 1036, 1041
Fnu4HI GCNGC 6 cut(s) 223, 226, 530, 939, 1098, 1144
FokI GGATG 2 cut(s) 308, 588
FriOI GRGCYC 3 cut(s) 120, 742, 1070
Fsp4HI GCNGC 6 cut(s) 223, 226, 530, 939, 1098, 1144
FspBI CTAG 2 cut(s) 428, 609
GlaI GCGC 1 cut(s) 328
GluI GCNGC 6 cut(s) 223, 226, 530, 939, 1098, 1144
HaeIII GGCC 6 cut(s) 98, 144, 182, 356, 570, 1000
HapII CCGG 5 cut(s) 179, 186, 195, 534, 886
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 329
Hin1II CATG 6 cut(s) 103, 440, 690, 884, 1040, 1045
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 7 cut(s) 4, 70, 127, 175, 198, 466, 613
HpaII CCGG 5 cut(s) 179, 186, 195, 534, 886
HphI GGTGA 3 cut(s) 327, 680, 716
Hpy188I TCNGA 5 cut(s) 122, 132, 320, 814, 1138
Hpy188III TCNNGA 6 cut(s) 293, 428, 437, 500, 649, 881
HpyAV CCTTC 3 cut(s) 220, 1011, 1018
HpyCH4III ACNGT 7 cut(s) 49, 238, 316, 511, 551, 646, 733
HpyF10VI GCNNNNNNNGC 4 cut(s) 380, 935, 947, 1097
HpyF3I CTNAG 1 cut(s) 700
Hsp92II CATG 6 cut(s) 103, 440, 690, 884, 1040, 1045
HspAI GCGC 1 cut(s) 327
Kzo9I GATC 4 cut(s) 320, 787, 809, 1078
LmnI GCTCC 5 cut(s) 123, 162, 935, 1065, 1126
Lsp1109I GCAGC 5 cut(s) 234, 516, 925, 1109, 1130
LweI GCATC 3 cut(s) 361, 610, 937
MaeI CTAG 2 cut(s) 428, 609
MaeIII GTNAC 4 cut(s) 333, 668, 719, 772
MalI GATC 4 cut(s) 322, 789, 811, 1080
MboI GATC 4 cut(s) 320, 787, 809, 1078
MboII GAAGA 4 cut(s) 51, 261, 598, 717
MhlI GDGCHC 3 cut(s) 120, 742, 1070
MlsI TGGCCA 2 cut(s) 98, 356
MluCI AATT 4 cut(s) 347, 384, 1010, 1051
MluNI TGGCCA 2 cut(s) 98, 356
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 1 cut(s) 443
MnlI CCTC 9 cut(s) 26, 73, 84, 99, 176, 271, 424, 1014, 1069
Mox20I TGGCCA 2 cut(s) 98, 356
MroXI GAANNNNTTC 1 cut(s) 542
MscI TGGCCA 2 cut(s) 98, 356
MseI TTAA 3 cut(s) 750, 797, 1116
Msp20I TGGCCA 2 cut(s) 98, 356
MspA1I CMGCKG 1 cut(s) 225
MspI CCGG 5 cut(s) 179, 186, 195, 534, 886
MspR9I CCNGG 4 cut(s) 172, 534, 535, 1071
MvaI CCWGG 2 cut(s) 172, 1071
MwoI GCNNNNNNNGC 4 cut(s) 380, 935, 947, 1097
NciI CCSGG 2 cut(s) 534, 535
NcoI CCATGG 1 cut(s) 99
NdeII GATC 4 cut(s) 320, 787, 809, 1078
NlaIII CATG 6 cut(s) 103, 440, 690, 884, 1040, 1045
NlaIV GGNNCC 2 cut(s) 164, 1067
NmuCI GTSAC 3 cut(s) 333, 668, 719
NspI RCATGY 1 cut(s) 690
PagI TCATGA 2 cut(s) 436, 880
PasI CCCWGGG 1 cut(s) 1070
PcsI WCGNNNNNNNCGW 1 cut(s) 969
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 6 cut(s) 4, 127, 175, 198, 466, 613
PflMI CCANNNNNTGG 1 cut(s) 1042
PinAI ACCGGT 1 cut(s) 185
PkrI GCNGC 6 cut(s) 224, 227, 531, 940, 1099, 1145
Ple19I CGATCG 1 cut(s) 323
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 170, 1069
PspEI GGTNACC 1 cut(s) 333
PspGI CCWGG 2 cut(s) 170, 1069
PspN4I GGNNCC 2 cut(s) 164, 1067
PspPI GGNCC 2 cut(s) 142, 998
PstI CTGCAG 4 cut(s) 224, 782, 943, 1099
PvuI CGATCG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 750, 797, 1116
SatI GCNGC 6 cut(s) 223, 226, 530, 939, 1098, 1144
Sau3AI GATC 4 cut(s) 320, 787, 809, 1078
Sau96I GGNCC 2 cut(s) 142, 998
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 4 cut(s) 172, 534, 535, 1071
SduI GDGCHC 3 cut(s) 120, 742, 1070
SfaNI GCATC 3 cut(s) 361, 610, 937
SfcI CTRYAG 5 cut(s) 137, 220, 778, 939, 1095
SgrAI CRCCGGYG 1 cut(s) 185
SmaI CCCGGG 1 cut(s) 535
Sse9I AATT 4 cut(s) 347, 384, 1010, 1051
SsiI CCGC 2 cut(s) 225, 495
SspI AATATT 1 cut(s) 894
SspMI CTAG 2 cut(s) 428, 609
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 4 cut(s) 170, 532, 533, 1069
StyI CCWWGG 2 cut(s) 99, 848
TaaI ACNGT 7 cut(s) 49, 238, 316, 511, 551, 646, 733
TaqI TCGA 3 cut(s) 369, 499, 546
TasI AATT 4 cut(s) 347, 384, 1010, 1051
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 6 cut(s) 4, 127, 175, 198, 466, 613
Tru1I TTAA 3 cut(s) 750, 797, 1116
Tru9I TTAA 3 cut(s) 750, 797, 1116
TscAI CASTG 6 cut(s) 364, 602, 649, 763, 787, 937
TseFI GTSAC 3 cut(s) 333, 668, 719
TseI GCWGC 5 cut(s) 222, 529, 938, 1097, 1143
Tsp45I GTSAC 3 cut(s) 333, 668, 719
TspDTI ATGAA 6 cut(s) 312, 426, 453, 893, 990, 993
TspMI CCCGGG 1 cut(s) 533
TspRI CASTG 6 cut(s) 364, 602, 649, 763, 787, 937
Van91I CCANNNNNTGG 1 cut(s) 1042
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 1 cut(s) 690
XmaI CCCGGG 1 cut(s) 533
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 2 cut(s) 428, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.