Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0002243

Physical Location & Seq

Genomic Coordinates Forward (+)

3926279 .. 3937718

Gene Structure 11440 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3033 bp

ATGGCGTCAAAATATGGATCGAAATCAAAAAAATTAGGTTCGAGCAGTTCAAAGGCTGCTAATTCCCCTGCATCTTCGACTACAACGTCTTCAAAACAATATTTGGAAACATCTATTGAATGCCAGAGTTCTCCTGCGTCTTCTTCAGCTCGAAGTAAGCCACAGTACTTGTACTTGGAAAGATTACCGCAAGATGTGGAAAAATCCAAAGAAAACGTCACTGTGACGGTCCGCTTTCGTCCACTCAGCCCAAGGGAAATTCGCCAAGGAGAAGAAATTGCATGGTATGCAGATGGTGACACCATTTTGCGAAATGAGCATAATCCATCAATAGCTTATGCATATGATCGGGTATTTGGCCCTACCACCACAACGCGTCATGTCTATGATGTTGCTGCTCAGCATGTTATAAGTGGTGCTATGGAAGGCGTCAATGGTACAATATTTGCGTATGGGGTGACCAGCAGCGGGAAGACTCACACTATGCATGGTGATCAAAGGTCGCCTGGAATTATACCACTGGCTGTGAAGGATGCTTTTAGCATCATCCAAGAGACTCCAAATCGAGAGTATCTTCTGCGTGTGTCATACTTGGAAATCTATAATGAGGTTGTAAATGACTTGTTGAATCCAGCAGGACAGAATTTACGAATTAGAGAAGATGCCCAGGGCACCTTTGTTGAAGGAATAAAGGAAGAAGTTGTACTGTCCCCAGCTCACGCCCTTTCACTTATAGCAGCTGGAGAAGAGCACAGGCATGTTGGCTCAACAAATTTCAATCTATTCAGTAGCAGGAGCCACACGATATTTACGCTGACAATAGAAAGTAGTCCATGTGGGGAAAATTGTGAAGGTGAAGCTGTAAGTCTGTCACAATTGTTTTTAACCTCATCGATCTGGCAGGTTCTGAGAGCTCGAAAGCTGAAACTACTGGTGTCAGACGGAAAGCGTCATCGATCCTATATTAATAAAAGTTTGCTAACTCTAGGAACTGTTATATCAAAGTTGACCGATATGAGGGCTACTCATATACCATACAGGGATTCTAAATTGACAAGACTTCTTCAATCTTCTCTAAGTGGACATGGGCGTGTATCTCTCATCTGCACTGTTACTCCTTCATCAAGCAGTTCCGAAGAGACACATAATACATTGAAATTTGCACATCGGGCAAAACACATAGAAATTCAAGCAGCACAAAACAAGATTATTGATGAGAAGTCGCTCATTAAGAAATATCAAAATGAGATACGTAGTTTAAAGGAAGAGTTAGAAGAGTTGAAGAGGGGTATAGTCACAATTCCTCAACTAAAAGATGCTGGGGAAGATGATATTCTGCTCCTTAAACAAAAGTTAGAAGATGGCAAATACAAACTGCAATCAAGATTGGAACAAGAAGAAGAAGCCAAAGCTGCTTTGTTGGGAAGAATCCAAAGGCTGACAAAATTGATTTTGGTCTCAACAAAAGGAACACAATCATCAAGGTTTTCTCACCGACCTGATGTTAGAAGAAGACATTCCTTTGGTGAAGAGGAGCTTGCATACCTACCATACAAAAGGCGGGACTTGATTTTGGATGATGAAAGCGTTGAATTGTTTGTTCCTCCTCTTGAGGGGAGTACTGAAATGACTGATGACACACTGAAGGGGGAGAAGAAGACTCGGAAGCATGGACTTCTAAACTGGTTAAAGATACGGAAACGTGATAGTGGTACAGGGACGTTGACAAGCACAAGTGATAGATCCAGTGGAATAAAATCTACTAGTACACCTTCAACACCTCAAGCAGAAAGTGGTAATTTTCATGTAGAATCGAGGCTTTCACATTCAGCTCTTACAGAAAACTCTCCATCGGCTGATCTTTTGTCTGAGGCTAGAGAGGACAGAGAAGTTCGTCAGGAAAATTTCCTGGGACGAGAGGCACCTCTGACTAGCATGAAGTCAATTGATCGGATCAATCTTTTAAGGGAGCAGCGAAAAATCTTGTCTGATGAGGTAGCACTCCGTTCAAGTGCTCTGAAGCGGTTGTCCGAGGAGGCTTCAAGACACTCCGAGAAGGATGAAATTAAGATGGAGATGCGAAAGTTCAAAGATGAAATCAAGGTGAAGAATGAACAAATAGCTTTGGTGGAAAAGAAAATTGCAGAGTCCTTTTCACACAACAAAATGAGTCAACTGGAAGCATCCCAATCTATTGCTGAAGTGATGGAGCAATTGAATGAGAAGTCCTTTGAACTTGAGGTTAAAGCTGCTGATAATCGTATCATCCAAGAACAGCTGGAACAAAAGATTGGTGAATGCAAAGGATTGCAGGAAACAGTCGCCTCCTTGAAGCAGCAGCTTTCTGAGGCACTAGAGTTCAGAAATTTGAGCCCAGTAGTCACTTCTCGAACTGGTTCAAAAAATTTGCATGAGGAACTGGGCACAGAGAAAGATAATGCGGTGTTGAATGACAAAAATGAAGTTTTCCTCCTACTGAAGCAGGTTGAAGAACTGAAGCAGAAGGTGGCTGAACTGACGGAGTCAAAGGAACAGTTAGAAGTTCGGAACAAGAAATTGGTGGAGGAGAGCTCGTACGCCAAAGGTCTAGCCTCAGCTGCTGCTGTTGAGCTCAAGGCATTATCAGAAGAAGTTTCCAAACTCATGAACCAAAATGAAAAACAAGCTGCTGAGCTAGCCGCATCTAAGAGCTCTCCAACTCTGCGTCGAAATAGTAGCACAATCCGAAATGGTCGGAGAGATAGTCACATCAAACAGGACCAAAGCGGGCTTATCTCAGAAATGAAGAGAGAACTGGCAGTGAGCAAGGAGAGAGAACATTCATACGAGGCTGCTCTTACGGAGAAGGATAAAAGAGAGGCTGATCTTCAAAGAAGGGTTGAGGAATCCAAGCAAAGAGAAGCATACCTGGAAAATGAACTCGCCAACATGTGGGTTCTCGTGGCGAAATTAAAAAAATCCCACGGAACTGAAACTGATTCTTCGGACTCAACAAAAGAGACCCGACAAACTGATGGTTATGGGGGATGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0035371 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:1990752

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1011

Amino Acids

113.42

Weight (kDa)

7.97

Isoelectric Point (pI)

55.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	66 - 209	1.2e-19	Microtubule binding
Kinesin	PF00225	78 - 394	7e-91	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12020 AT3G12020 AT3G12020 AT3G12020 AT5G06670 AT5G06670 AT5G06670 AT5G06670
fragaria_vesca	FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780
malus_domestica	MD12G1054300.v1.1 MD14G1053100.v1.1
prunus_persica	Prupe.7G082400_v2.0.a1
pyrus_communis	pycom12g04730 pycom14g04410
rosa_chinensis	RchiOBHm_Chr3g0479141
rosa_laevigata	RLG00000023604
rosa_multiflora	Rmu_co8049936.1_g000001 Rmu_co8383491.1_g000001 Rmu_sc0002555.1_g000005
rosa_roxburghii	Rroxscaffold_6G00402700
rosa_rugosa	Rorug03G0171300 Rorug03G0171400
rosa_samantha	Rh3AG222600 Rh3BG255100 Rh3CG250700 Rh3DG247100 Rh6AG090700 Rh6BG153100 Rh6CG078500 Rh6DG074300 Rh6DG137500
rosa_wichuraiana	Rw0G001170 Rw1G014040 Rw3G019950 Rw6G024570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	410
AasI	GACNNNNNNGTC	1 cut(s)	85
Acc36I	ACCTGC	2 cut(s)	892, 2473
AccB1I	GGYRCC	2 cut(s)	671, 1921
AccB7I	CCANNNNNTGG	1 cut(s)	2931
AccII	CGCG	1 cut(s)	376
AciI	CCGC	8 cut(s)	188, 232, 468, 1561, 2023, 2441, 2679, 2766
AclWI	GGATC	4 cut(s)	25, 951, 1737, 1961
AcsI	RAATTY	8 cut(s)	258, 643, 772, 1157, 1185, 1903, 2365, 2404
AcuI	CTGAAG	6 cut(s)	129, 1664, 2039, 2220, 2498, 2516
AcyI	GRCGYC	2 cut(s)	5, 429
AfaI	GTAC	8 cut(s)	167, 173, 439, 705, 1621, 1714, 1768, 2576
AfiI	CCNNNNNNNGG	4 cut(s)	468, 1017, 1613, 2931
AflIII	ACRYGT	2 cut(s)	374, 2928
AhlI	ACTAGT	1 cut(s)	1763
AjnI	CCWGG	4 cut(s)	505, 666, 1908, 2907
AjuI	GAANNNNNNNTTGG	4 cut(s)	2274, 2306, 2540, 2572
Alw21I	GWGCWC	6 cut(s)	753, 916, 2017, 2573, 2613, 2693
Alw26I	GTCTC	4 cut(s)	548, 1133, 1462, 2993
AlwI	GGATC	4 cut(s)	25, 951, 1737, 1961
AlwNI	CAGNNNCTG	2 cut(s)	907, 2600
AoxI	GGCC	1 cut(s)	358
ApoI	RAATTY	8 cut(s)	258, 643, 772, 1157, 1185, 1903, 2365, 2404
ArsI	GACNNNNNNTTYG	2 cut(s)	201, 233
AseI	ATTAAT	1 cut(s)	966
Asp700I	GAANNNNTTC	2 cut(s)	1516, 2395
AspS9I	GGNCC	3 cut(s)	229, 359, 2758
AsuHPI	GGTGA	8 cut(s)	308, 469, 503, 866, 1484, 1538, 2116, 2306
AsuNHI	GCTAGC	1 cut(s)	2674
AvaII	GGWCC	2 cut(s)	229, 2758
BaeGI	GKGCMC	2 cut(s)	674, 2426
BanI	GGYRCC	2 cut(s)	671, 1921
BanII	GRGCYC	5 cut(s)	916, 2375, 2573, 2613, 2693
BarI	GAAGNNNNNNTAC	2 cut(s)	687, 719
BauI	CACGAG	1 cut(s)	2939
BbsI	GAAGAC	5 cut(s)	81, 132, 479, 1519, 1664
Bbv12I	GWGCWC	6 cut(s)	753, 916, 2017, 2573, 2613, 2693
BbvCI	CCTCAGC	1 cut(s)	2593
BccI	CCATC	8 cut(s)	287, 334, 1355, 1858, 2065, 2200, 3008, 3021
BciT130I	CCWGG	4 cut(s)	507, 668, 1910, 2909
BclI	TGATCA	1 cut(s)	493
BcoDI	GTCTC	4 cut(s)	548, 1133, 1462, 2993
BcuI	ACTAGT	1 cut(s)	1763
BfaI	CTAG	7 cut(s)	986, 1764, 1875, 1932, 2354, 2588, 2675
BfuAI	ACCTGC	2 cut(s)	892, 2473
BlpI	GCTNAGC	2 cut(s)	399, 2670
BmcAI	AGTACT	2 cut(s)	167, 1621
Bme1390I	CCNGG	4 cut(s)	507, 668, 1910, 2909
Bme18I	GGWCC	2 cut(s)	229, 2758
BmgT120I	GGNCC	3 cut(s)	229, 359, 2758
BmiI	GGNNCC	3 cut(s)	673, 797, 1923
BmrFI	CCNGG	4 cut(s)	507, 668, 1910, 2909
BmrI	ACTGGG	2 cut(s)	2369, 2429
BmsI	GCATC	8 cut(s)	80, 523, 552, 652, 1306, 2067, 2192, 2690
BmtI	GCTAGC	1 cut(s)	2678
BmuI	ACTGGG	2 cut(s)	2369, 2429
BpiI	GAAGAC	5 cut(s)	81, 132, 479, 1519, 1664
BplI	GAGNNNNNCTC	4 cut(s)	1009, 1041, 2555, 2587
BpmI	CTGGAG	1 cut(s)	762
Bpu10I	CCTNAGC	1 cut(s)	2593
Bpu1102I	GCTNAGC	2 cut(s)	399, 2670
BpuEI	CTTGAG	4 cut(s)	1631, 1767, 2258, 2597
Bsa29I	ATCGAT	2 cut(s)	893, 955
BsaAI	YACGTR	1 cut(s)	1253
BsaBI	GATNNNNATC	1 cut(s)	22
BsaHI	GRCGYC	2 cut(s)	5, 429
BsaI	GGTCTC	2 cut(s)	1462, 2993
BsaJI	CCNNGG	7 cut(s)	251, 265, 666, 667, 1909, 2031, 2962
BsaXI	ACNNNNNCTCC	6 cut(s)	1099, 1129, 2558, 2588, 2728, 2758
Bsc4I	CCNNNNNNNGG	4 cut(s)	468, 1017, 1613, 2931
Bse1I	ACTGG	9 cut(s)	525, 936, 1688, 1746, 2181, 2375, 2398, 2424, 2799
Bse8I	GATNNNNATC	1 cut(s)	22
BseBI	CCWGG	4 cut(s)	507, 668, 1910, 2909
BseCI	ATCGAT	2 cut(s)	893, 955
BseDI	CCNNGG	7 cut(s)	251, 265, 666, 667, 1909, 2031, 2962
BseGI	GGATG	7 cut(s)	538, 546, 1582, 2065, 2183, 2265, 3032
BseJI	GATNNNNATC	1 cut(s)	22
BseLI	CCNNNNNNNGG	4 cut(s)	468, 1017, 1613, 2931
BseMII	CTCAG	8 cut(s)	259, 413, 899, 1860, 2337, 2607, 2661, 2790
BseNI	ACTGG	9 cut(s)	525, 936, 1688, 1746, 2181, 2375, 2398, 2424, 2799
BseRI	GAGGAG	4 cut(s)	1547, 1596, 2048, 2579
BseSI	GKGCMC	2 cut(s)	674, 2426
BseYI	CCCAGC	2 cut(s)	712, 1319
BsgI	GTGCAG	1 cut(s)	1090
Bsh1236I	CGCG	1 cut(s)	376
BshFI	GGCC	1 cut(s)	360
BshNI	GGYRCC	2 cut(s)	671, 1921
BshVI	ATCGAT	2 cut(s)	893, 955
BsiHKAI	GWGCWC	6 cut(s)	753, 916, 2017, 2573, 2613, 2693
BsiWI	CGTACG	1 cut(s)	2574
BslFI	GGGAC	4 cut(s)	694, 1577, 1732, 1926
BslI	CCNNNNNNNGG	4 cut(s)	468, 1017, 1613, 2931
BsmAI	GTCTC	4 cut(s)	548, 1133, 1462, 2993
BsmFI	GGGAC	4 cut(s)	694, 1577, 1732, 1926
BsmI	GAATGC	2 cut(s)	125, 2303
BsnI	GGCC	1 cut(s)	360
Bso31I	GGTCTC	2 cut(s)	1462, 2993
Bsp1286I	GDGCHC	9 cut(s)	674, 753, 916, 2017, 2375, 2426, 2573, 2613, 2693
Bsp1720I	GCTNAGC	2 cut(s)	399, 2670
BspACI	CCGC	8 cut(s)	188, 232, 468, 1561, 2023, 2441, 2679, 2766
BspANI	GGCC	1 cut(s)	360
BspCNI	CTCAG	8 cut(s)	258, 412, 900, 1861, 2338, 2606, 2662, 2789
BspDI	ATCGAT	2 cut(s)	893, 955
BspFNI	CGCG	1 cut(s)	376
BspHI	TCATGA	1 cut(s)	2643
BspLI	GGNNCC	3 cut(s)	673, 797, 1923
BspMI	ACCTGC	2 cut(s)	892, 2473
BspOI	GCTAGC	1 cut(s)	2678
BspPI	GGATC	4 cut(s)	25, 951, 1737, 1961
BspQI	GCTCTTC	1 cut(s)	741
BspT107I	GGYRCC	2 cut(s)	671, 1921
BspTNI	GGTCTC	2 cut(s)	1462, 2993
BsrI	ACTGG	9 cut(s)	525, 936, 1688, 1746, 2181, 2375, 2398, 2424, 2799
BssECI	CCNNGG	7 cut(s)	251, 265, 666, 667, 1909, 2031, 2962
BssNI	GRCGYC	2 cut(s)	5, 429
BssSI	CACGAG	1 cut(s)	2939
BssT1I	CCWWGG	2 cut(s)	251, 265
Bst2BI	CACGAG	1 cut(s)	2939
Bst2UI	CCWGG	4 cut(s)	507, 668, 1910, 2909
Bst4CI	ACNGT	8 cut(s)	165, 223, 229, 708, 994, 1111, 2320, 2535
Bst6I	CTCTTC	7 cut(s)	741, 1131, 1260, 1269, 1277, 1524, 2780
BstACI	GRCGYC	2 cut(s)	5, 429
BstAPI	GCANNNNNTGC	1 cut(s)	287
BstBAI	YACGTR	1 cut(s)	1253
BstC8I	GCNNGC	3 cut(s)	1539, 2676, 2768
BstDSI	CCRYGG	1 cut(s)	2962
BstEII	GGTNACC	1 cut(s)	457
BstF5I	GGATG	7 cut(s)	538, 546, 1582, 2065, 2183, 2265, 3032
BstFNI	CGCG	1 cut(s)	376
BstMAI	GTCTC	4 cut(s)	548, 1133, 1462, 2993
BstMWI	GCNNNNNNNGC	6 cut(s)	287, 316, 1169, 1412, 2597, 2675
BstNI	CCWGG	4 cut(s)	507, 668, 1910, 2909
BstNSI	RCATGY	3 cut(s)	407, 761, 2932
BstPI	GGTNACC	1 cut(s)	457
BstSCI	CCNGG	4 cut(s)	505, 666, 1908, 2907
BstSLI	GKGCMC	2 cut(s)	674, 2426
BstSNI	TACGTA	1 cut(s)	1253
BstUI	CGCG	1 cut(s)	376
BstV2I	GAAGAC	5 cut(s)	81, 132, 479, 1519, 1664
BstX2I	RGATCY	1 cut(s)	1742
BstYI	RGATCY	1 cut(s)	1742
Bsu15I	ATCGAT	2 cut(s)	893, 955
BsuRI	GGCC	1 cut(s)	360
BsuTUI	ATCGAT	2 cut(s)	893, 955
BtgI	CCRYGG	1 cut(s)	2962
BtsCI	GGATG	7 cut(s)	538, 546, 1582, 2065, 2183, 2265, 3032
BtsI	GCAGTG	1 cut(s)	2805
BtsIMutI	CAGTG	6 cut(s)	219, 518, 1107, 1640, 1753, 2805
BveI	ACCTGC	2 cut(s)	892, 2473
Cac8I	GCNNGC	3 cut(s)	1539, 2676, 2768
CaiI	CAGNNNCTG	2 cut(s)	907, 2600
CciI	TCATGA	1 cut(s)	2643
Cfr13I	GGNCC	3 cut(s)	229, 359, 2758
ClaI	ATCGAT	2 cut(s)	893, 955
CpoI	CGGWCCG	1 cut(s)	229
CseI	GACGC	5 cut(s)	126, 365, 418, 938, 2693
Csp6I	GTAC	8 cut(s)	166, 172, 438, 704, 1620, 1713, 1767, 2575
CspCI	CAANNNNNGTGG	2 cut(s)	150, 185
CspI	CGGWCCG	1 cut(s)	229
CviQI	GTAC	8 cut(s)	166, 172, 438, 704, 1620, 1713, 1767, 2575
DraI	TTTAAA	1 cut(s)	1260
DrdI	GACNNNNNNGTC	1 cut(s)	85
DseDI	GACNNNNNNGTC	1 cut(s)	85
Eam1104I	CTCTTC	7 cut(s)	741, 1131, 1260, 1269, 1277, 1524, 2780
EarI	CTCTTC	7 cut(s)	741, 1131, 1260, 1269, 1277, 1524, 2780
Ecl136II	GAGCTC	4 cut(s)	914, 2571, 2611, 2691
Eco105I	TACGTA	1 cut(s)	1253
Eco130I	CCWWGG	2 cut(s)	251, 265
Eco24I	GRGCYC	5 cut(s)	916, 2375, 2573, 2613, 2693
Eco31I	GGTCTC	2 cut(s)	1462, 2993
Eco47I	GGWCC	2 cut(s)	229, 2758
Eco53kI	GAGCTC	4 cut(s)	914, 2571, 2611, 2691
Eco57I	CTGAAG	6 cut(s)	129, 1664, 2039, 2220, 2498, 2516
Eco91I	GGTNACC	1 cut(s)	457
EcoICRI	GAGCTC	4 cut(s)	914, 2571, 2611, 2691
EcoO65I	GGTNACC	1 cut(s)	457
EcoRII	CCWGG	4 cut(s)	505, 666, 1908, 2907
EcoT14I	CCWWGG	2 cut(s)	251, 265
EcoT22I	ATGCAT	2 cut(s)	343, 489
EcoT38I	GRGCYC	5 cut(s)	916, 2375, 2573, 2613, 2693
ErhI	CCWWGG	2 cut(s)	251, 265
FalI	AAGNNNNNCTT	2 cut(s)	1521, 1553
FaqI	GGGAC	4 cut(s)	694, 1577, 1732, 1926
FauI	CCCGC	3 cut(s)	461, 1554, 2759
FauNDI	CATATG	1 cut(s)	343
FbaI	TGATCA	1 cut(s)	493
FokI	GGATG	6 cut(s)	533, 545, 1589, 2072, 2170, 2252
FriOI	GRGCYC	5 cut(s)	916, 2375, 2573, 2613, 2693
FspBI	CTAG	7 cut(s)	986, 1764, 1875, 1932, 2354, 2588, 2675
GsaI	CCCAGC	2 cut(s)	716, 1323
GsuI	CTGGAG	1 cut(s)	762
HaeIII	GGCC	1 cut(s)	360
HgaI	GACGC	5 cut(s)	126, 365, 418, 938, 2693
Hin1I	GRCGYC	2 cut(s)	5, 429
HincII	GTYRAC	3 cut(s)	1008, 1725, 2174
HindII	GTYRAC	3 cut(s)	1008, 1725, 2174
HphI	GGTGA	8 cut(s)	308, 469, 503, 866, 1484, 1538, 2116, 2306
Hpy166II	GTNNAC	6 cut(s)	242, 1008, 1082, 1725, 1769, 2174
Hpy188III	TCNNGA	7 cut(s)	566, 1383, 1610, 1898, 2043, 2388, 2644
Hpy8I	GTNNAC	6 cut(s)	242, 1008, 1082, 1725, 1769, 2174
Hpy99I	CGWCG	1 cut(s)	2709
HpyCH4III	ACNGT	8 cut(s)	165, 223, 229, 708, 994, 1111, 2320, 2535
HpyCH4IV	ACGT	5 cut(s)	86, 216, 1252, 1702, 1721
HpyF10VI	GCNNNNNNNGC	6 cut(s)	287, 316, 1169, 1412, 2597, 2675
HpySE526I	ACGT	5 cut(s)	86, 216, 1252, 1702, 1721
Hsp92I	GRCGYC	2 cut(s)	5, 429
Ksp22I	TGATCA	1 cut(s)	493
LguI	GCTCTTC	1 cut(s)	741
LmnI	GCTCC	5 cut(s)	795, 1344, 1534, 1969, 2209
LweI	GCATC	8 cut(s)	80, 523, 552, 652, 1306, 2067, 2192, 2690
MaeI	CTAG	7 cut(s)	986, 1764, 1875, 1932, 2354, 2588, 2675
MaeII	ACGT	5 cut(s)	86, 216, 1252, 1702, 1721
MaeIII	GTNAC	9 cut(s)	217, 223, 296, 457, 870, 1111, 1294, 2380, 2744
MfeI	CAATTG	3 cut(s)	875, 1944, 2213
MflI	RGATCY	1 cut(s)	1742
MhlI	GDGCHC	9 cut(s)	674, 753, 916, 2017, 2375, 2426, 2573, 2613, 2693
MluI	ACGCGT	1 cut(s)	374
MlyI	GAGTC	7 cut(s)	469, 550, 1654, 2156, 2179, 2531, 2981
MmeI	TCCRAC	2 cut(s)	2714, 2720
Mph1103I	ATGCAT	2 cut(s)	343, 489
MroXI	GAANNNNTTC	2 cut(s)	1516, 2395
MslI	CAYNNNNRTG	3 cut(s)	384, 756, 1089
MspA1I	CMGCKG	4 cut(s)	468, 740, 2278, 2597
MspR9I	CCNGG	4 cut(s)	507, 668, 1910, 2909
MunI	CAATTG	3 cut(s)	875, 1944, 2213
Mva1269I	GAATGC	2 cut(s)	125, 2303
MvaI	CCWGG	4 cut(s)	507, 668, 1910, 2909
MvnI	CGCG	1 cut(s)	376
MwoI	GCNNNNNNNGC	6 cut(s)	287, 316, 1169, 1412, 2597, 2675
NdeI	CATATG	1 cut(s)	343
NheI	GCTAGC	1 cut(s)	2674
NlaIV	GGNNCC	3 cut(s)	673, 797, 1923
NmuCI	GTSAC	8 cut(s)	217, 223, 296, 457, 870, 1294, 2380, 2744
NsiI	ATGCAT	2 cut(s)	343, 489
NspI	RCATGY	3 cut(s)	407, 761, 2932
PagI	TCATGA	1 cut(s)	2643
PasI	CCCWGGG	1 cut(s)	667
PciI	ACATGT	1 cut(s)	2928
PciSI	GCTCTTC	1 cut(s)	741
PcsI	WCGNNNNNNNCGW	1 cut(s)	83
PctI	GAATGC	2 cut(s)	125, 2303
PdmI	GAANNNNTTC	2 cut(s)	1516, 2395
PfeI	GAWTC	6 cut(s)	628, 1043, 1428, 1811, 2885, 2978
Pfl23II	CGTACG	1 cut(s)	2574
PflFI	GACNNNGTC	1 cut(s)	2521
PflMI	CCANNNNNTGG	1 cut(s)	2931
PleI	GAGTC	7 cut(s)	469, 550, 1654, 2155, 2178, 2530, 2981
PpsI	GAGTC	7 cut(s)	469, 550, 1654, 2155, 2178, 2530, 2981
Ppu21I	YACGTR	1 cut(s)	1253
PscI	ACATGT	1 cut(s)	2928
PshBI	ATTAAT	1 cut(s)	966
PsiI	TTATAA	1 cut(s)	410
Psp124BI	GAGCTC	4 cut(s)	916, 2573, 2613, 2693
Psp6I	CCWGG	4 cut(s)	505, 666, 1908, 2907
PspEI	GGTNACC	1 cut(s)	457
PspFI	CCCAGC	2 cut(s)	712, 1319
PspGI	CCWGG	4 cut(s)	505, 666, 1908, 2907
PspLI	CGTACG	1 cut(s)	2574
PspN4I	GGNNCC	3 cut(s)	673, 797, 1923
PspPI	GGNCC	3 cut(s)	229, 359, 2758
PstNI	CAGNNNCTG	2 cut(s)	907, 2600
PsuI	RGATCY	1 cut(s)	1742
PsyI	GACNNNGTC	1 cut(s)	2521
PvuII	CAGCTG	3 cut(s)	740, 2278, 2597
RsaI	GTAC	8 cut(s)	167, 173, 439, 705, 1621, 1714, 1768, 2576
RsaNI	GTAC	8 cut(s)	166, 172, 438, 704, 1620, 1713, 1767, 2575
RseI	CAYNNNNRTG	3 cut(s)	384, 756, 1089
Rsr2I	CGGWCCG	1 cut(s)	229
RsrII	CGGWCCG	1 cut(s)	229
SacI	GAGCTC	4 cut(s)	916, 2573, 2613, 2693
SapI	GCTCTTC	1 cut(s)	741
Sau96I	GGNCC	3 cut(s)	229, 359, 2758
ScaI	AGTACT	2 cut(s)	167, 1621
SchI	GAGTC	7 cut(s)	469, 550, 1654, 2156, 2179, 2531, 2981
ScrFI	CCNGG	4 cut(s)	507, 668, 1910, 2909
SduI	GDGCHC	9 cut(s)	674, 753, 916, 2017, 2375, 2426, 2573, 2613, 2693
SfaNI	GCATC	8 cut(s)	80, 523, 552, 652, 1306, 2067, 2192, 2690
SinI	GGWCC	2 cut(s)	229, 2758
SmiMI	CAYNNNNRTG	3 cut(s)	384, 756, 1089
SmlI	CTYRAG	4 cut(s)	1610, 1782, 2237, 2612
SmoI	CTYRAG	4 cut(s)	1610, 1782, 2237, 2612
SnaBI	TACGTA	1 cut(s)	1253
SpeI	ACTAGT	1 cut(s)	1763
SsiI	CCGC	8 cut(s)	188, 232, 468, 1561, 2023, 2441, 2679, 2766
SspI	AATATT	2 cut(s)	101, 444
SspMI	CTAG	7 cut(s)	986, 1764, 1875, 1932, 2354, 2588, 2675
SstI	GAGCTC	4 cut(s)	916, 2573, 2613, 2693
StyD4I	CCNGG	4 cut(s)	505, 666, 1908, 2907
StyI	CCWWGG	2 cut(s)	251, 265
TaaI	ACNGT	8 cut(s)	165, 223, 229, 708, 994, 1111, 2320, 2535
TaiI	ACGT	5 cut(s)	89, 219, 1255, 1705, 1724
TaqII	GACCGA	1 cut(s)	1025
TatI	WGTACW	5 cut(s)	165, 171, 703, 1619, 1766
TauI	GCSGC	1 cut(s)	2681
TfiI	GAWTC	6 cut(s)	628, 1043, 1428, 1811, 2885, 2978
TscAI	CASTG	6 cut(s)	226, 525, 1114, 1647, 1753, 2805
TseFI	GTSAC	8 cut(s)	217, 223, 296, 457, 870, 1294, 2380, 2744
Tsp45I	GTSAC	8 cut(s)	217, 223, 296, 457, 870, 1294, 2380, 2744
TspGWI	ACGGA	6 cut(s)	957, 1711, 1994, 2534, 2855, 2979
TspRI	CASTG	6 cut(s)	226, 525, 1114, 1647, 1753, 2805
Tth111I	GACNNNGTC	1 cut(s)	2521
Van91I	CCANNNNNTGG	1 cut(s)	2931
VpaK11BI	GGWCC	2 cut(s)	229, 2758
VspI	ATTAAT	1 cut(s)	966
XapI	RAATTY	8 cut(s)	258, 643, 772, 1157, 1185, 1903, 2365, 2404
XceI	RCATGY	3 cut(s)	407, 761, 2932
XmnI	GAANNNNTTC	2 cut(s)	1516, 2395
XspI	CTAG	7 cut(s)	986, 1764, 1875, 1932, 2354, 2588, 2675
ZrmI	AGTACT	2 cut(s)	167, 1621
Zsp2I	ATGCAT	2 cut(s)	343, 489

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.