pycom14g04590

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011978
Physical Location & Seq
Reverse (-)
4226299 .. 4228335
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGAGGATTCGGAAACGACAGGTACCCTTCCCTCTGTCCTCTCTCTCTCCGGTGCCTTTATCAGATCCCCTCCTCCACGACCTCAACTACTCACCACCACCCGTGGTGCAACTCCACCCCCACCACCGCCATGATCCTCCAAATCCCTCCCACCCAGTTGAGAAAACTGTCCATGTGGGCCACCCCCCTCCTCAGCCCTTCGATCAGCGAGTCTTTCCGATCGGACGAGCAGGCAAGGGTTCTGATGGTTCTGGGGAGAATAAGAACAAAGAGAACAAGCAAGATTGTGGTTTGGAGATATCGATATTGAAAGGGGAAGAAGATGAGAGAGAATCCATAAAGGGAGGAGATCAGAAGGGAAATGATGATGATGATATCAGGGAGGAAAGCATATTGGTTGCTGAAACGTTAATGGGGTTTGCTTCGGGATCATCTAGTTCTTCTCACCCAGGAGAGAAAGCGTTTCCATTGAAGAAGCGAAGAGGAAGCTTCGAGAGAGGAGCCAATAATATCGGACAGACGATAATGGATGATCAGAAAGACAAAAAATCAATGAAAACGAAGACGAACAAGAAAAGGGAACCTCGACAGCAGCAAAATGACGATGTTCAACAGAAGCAAGACGATCATGTGATTGATGGCAATAGAGTCACTACTAGTGCTAGTGCAACAAGAAAGAGGGCAAGGGGAGGTGCACTCATGGAGGGGTCGCGGTGCAGTAGAGTTAACGGAAGAGGATGGAGGTGTTGCCAACAAACGCTTGTTGGTTACTCTCTTTGTGAGCATCACTTGGGGAAGGGTAGGTTAAGGAGCATGAATAGTGTCCGAAGCCGGTCGACGGCTGCGGCAACTGCTACTACCACTACTAGTAGTACTAGAATTGCTTCTACTGAGACTGAATTCAAGCAACTATCAGCGTCTAGAATATTATCCGTGGAGTCATCACCGCCGCAGAAAGAAGACACGAAAGCTGTTTTGCTGCTGGACGATGGTGATATTGGTGAAGGTAAAGGAAATGAAGGTGAAGATGAAGAGATCAAGAAGCCGTTGCTGGTTACTAAGAAGCGGATGAAGCTTGGGATCGTTAAAGCCCGATCCATGAGCAGCTTGCTAGGCCAAACGAATAGTCGTGCAATGGCTGATGAGGATGATGATAATAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

389

Amino Acids

42.69

Weight (kDa)

9.13

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 235 - 275 1.2e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 22
AccB1I GGYRCC 2 cut(s) 22, 52
AccI GTMKAC 1 cut(s) 836
AccII CGCG 1 cut(s) 712
AciI CCGC 6 cut(s) 127, 712, 845, 947, 950, 1066
AclI AACGTT 1 cut(s) 407
AclWI GGATC 5 cut(s) 59, 128, 436, 1088, 1089
AcsI RAATTY 1 cut(s) 899
AfaI GTAC 2 cut(s) 24, 874
AfiI CCNNNNNNNGG 1 cut(s) 838
AgsI TTSAA 4 cut(s) 310, 472, 611, 904
AhlI ACTAGT 2 cut(s) 656, 866
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 4 cut(s) 489, 971, 1075, 1107
AluI AGCT 4 cut(s) 489, 971, 1075, 1107
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 1 cut(s) 887
Alw44I GTGCAC 1 cut(s) 693
AlwI GGATC 5 cut(s) 59, 128, 436, 1088, 1089
AoxI GGCC 2 cut(s) 178, 1114
ApaLI GTGCAC 1 cut(s) 693
ApeKI GCWGC 4 cut(s) 592, 842, 979, 1104
ApoI RAATTY 1 cut(s) 899
ArsI GACNNNNNNTTYG 2 cut(s) 1111, 1143
Asp700I GAANNNNTTC 1 cut(s) 883
Asp718I GGTACC 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 178
AsuHPI GGTGA 6 cut(s) 84, 437, 936, 1004, 1013, 1034
BaeGI GKGCMC 1 cut(s) 697
BanI GGYRCC 2 cut(s) 22, 52
BbsI GAAGAC 2 cut(s) 569, 966
Bbv12I GWGCWC 1 cut(s) 697
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 4 cut(s) 604, 829, 966, 1116
BccI CCATC 4 cut(s) 239, 632, 732, 983
BceAI ACGGC 2 cut(s) 855, 1030
BciT130I CCWGG 1 cut(s) 450
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 887
BcuI ACTAGT 2 cut(s) 656, 866
BfaI CTAG 7 cut(s) 435, 657, 663, 867, 876, 921, 1112
BisI GCNGC 6 cut(s) 593, 843, 846, 950, 980, 1105
BlsI GCNGC 6 cut(s) 594, 844, 847, 951, 981, 1106
BmcAI AGTACT 1 cut(s) 874
Bme1390I CCNGG 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 178
BmiI GGNNCC 4 cut(s) 24, 54, 502, 582
BmrFI CCNGG 1 cut(s) 450
BmrI ACTGGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 793
BmuI ACTGGG 1 cut(s) 149
BpiI GAAGAC 2 cut(s) 569, 966
BplI GAGNNNNNCTC 2 cut(s) 681, 713
Bpu10I CCTNAGC 1 cut(s) 192
Bsa29I ATCGAT 1 cut(s) 302
BsaJI CCNNGG 3 cut(s) 102, 448, 933
BsaWI WCCGGW 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 838
Bse118I RCCGGY 1 cut(s) 831
Bse1I ACTGG 1 cut(s) 155
Bse3DI GCAATG 1 cut(s) 1140
BseBI CCWGG 1 cut(s) 450
BseCI ATCGAT 1 cut(s) 302
BseDI CCNNGG 3 cut(s) 102, 448, 933
BseGI GGATG 4 cut(s) 535, 743, 1074, 1153
BseLI CCNNNNNNNGG 1 cut(s) 838
BseMI GCAATG 1 cut(s) 1140
BseMII CTCAG 2 cut(s) 206, 882
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 4 cut(s) 62, 180, 360, 513
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 4 cut(s) 604, 829, 966, 1116
BsgI GTGCAG 1 cut(s) 736
Bsh1236I CGCG 1 cut(s) 712
Bsh1285I CGRYCG 2 cut(s) 222, 836
BshFI GGCC 2 cut(s) 180, 1116
BshNI GGYRCC 2 cut(s) 22, 52
BshVI ATCGAT 1 cut(s) 302
BsiEI CGRYCG 2 cut(s) 222, 836
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 2 cut(s) 50, 832
BslI CCNNNNNNNGG 1 cut(s) 838
BsmAI GTCTC 1 cut(s) 887
BsnI GGCC 2 cut(s) 180, 1116
Bsp1286I GDGCHC 1 cut(s) 697
BspACI CCGC 6 cut(s) 127, 712, 845, 947, 950, 1066
BspANI GGCC 2 cut(s) 180, 1116
BspCNI CTCAG 2 cut(s) 205, 883
BspDI ATCGAT 1 cut(s) 302
BspFNI CGCG 1 cut(s) 712
BspLI GGNNCC 4 cut(s) 24, 54, 502, 582
BspPI GGATC 5 cut(s) 59, 128, 436, 1088, 1089
BspT107I GGYRCC 2 cut(s) 22, 52
BsrDI GCAATG 1 cut(s) 1140
BsrFI RCCGGY 1 cut(s) 831
BsrI ACTGG 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 831
BssECI CCNNGG 3 cut(s) 102, 448, 933
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 3 cut(s) 475, 727, 1026
BstC8I GCNNGC 2 cut(s) 232, 1109
BstDEI CTNAG 3 cut(s) 192, 891, 1059
BstDSI CCRYGG 2 cut(s) 102, 933
BstF5I GGATG 4 cut(s) 535, 743, 1074, 1153
BstFNI CGCG 1 cut(s) 712
BstMAI GTCTC 1 cut(s) 887
BstMCI CGRYCG 2 cut(s) 222, 836
BstMWI GCNNNNNNNGC 3 cut(s) 851, 1072, 1113
BstNI CCWGG 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 448
BstSLI GKGCMC 1 cut(s) 697
BstUI CGCG 1 cut(s) 712
BstV1I GCAGC 4 cut(s) 604, 829, 966, 1116
BstV2I GAAGAC 2 cut(s) 569, 966
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
Bsu15I ATCGAT 1 cut(s) 302
BsuRI GGCC 2 cut(s) 180, 1116
BsuTUI ATCGAT 1 cut(s) 302
BtgI CCRYGG 2 cut(s) 102, 933
BtsCI GGATG 4 cut(s) 535, 743, 1074, 1153
Cac8I GCNNGC 2 cut(s) 232, 1109
Cfr10I RCCGGY 1 cut(s) 831
Cfr13I GGNCC 1 cut(s) 178
ClaI ATCGAT 1 cut(s) 302
CseI GACGC 1 cut(s) 906
Csp6I GTAC 2 cut(s) 23, 873
CviAII CATG 6 cut(s) 131, 173, 629, 700, 814, 1099
CviQI GTAC 2 cut(s) 23, 873
DdeI CTNAG 3 cut(s) 192, 891, 1059
Eam1104I CTCTTC 3 cut(s) 475, 727, 1026
EarI CTCTTC 3 cut(s) 475, 727, 1026
Eco32I GATATC 2 cut(s) 300, 376
EcoRI GAATTC 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 448
EcoRV GATATC 2 cut(s) 300, 376
FaeI CATG 6 cut(s) 134, 176, 632, 703, 817, 1102
FaiI YATR 8 cut(s) 132, 174, 338, 392, 630, 701, 815, 1100
FatI CATG 6 cut(s) 130, 172, 628, 699, 813, 1098
FbaI TGATCA 1 cut(s) 532
FblI GTMKAC 1 cut(s) 836
Fnu4HI GCNGC 6 cut(s) 593, 843, 846, 950, 980, 1105
FokI GGATG 4 cut(s) 542, 750, 1081, 1160
Fsp4HI GCNGC 6 cut(s) 593, 843, 846, 950, 980, 1105
FspBI CTAG 7 cut(s) 435, 657, 663, 867, 876, 921, 1112
GluI GCNGC 6 cut(s) 593, 843, 846, 950, 980, 1105
HaeIII GGCC 2 cut(s) 180, 1116
HapII CCGG 2 cut(s) 50, 832
HgaI GACGC 1 cut(s) 906
Hin1II CATG 6 cut(s) 134, 176, 632, 703, 817, 1102
HincII GTYRAC 2 cut(s) 727, 837
HindII GTYRAC 2 cut(s) 727, 837
HindIII AAGCTT 2 cut(s) 487, 1073
HinfI GANTC 5 cut(s) 7, 210, 332, 648, 938
HpaI GTTAAC 1 cut(s) 727
HpaII CCGG 2 cut(s) 50, 832
HphI GGTGA 6 cut(s) 84, 437, 936, 1004, 1013, 1034
Hpy166II GTNNAC 3 cut(s) 695, 727, 837
Hpy188I TCNGA 9 cut(s) 12, 64, 219, 224, 244, 354, 515, 537, 827
Hpy188III TCNNGA 4 cut(s) 426, 493, 921, 1039
Hpy8I GTNNAC 3 cut(s) 695, 727, 837
Hpy99I CGWCG 1 cut(s) 841
HpyAV CCTTC 6 cut(s) 37, 208, 349, 790, 998, 1013
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 5 cut(s) 109, 668, 695, 717, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 851, 1072, 1113
HpyF3I CTNAG 3 cut(s) 192, 891, 1059
HpySE526I ACGT 1 cut(s) 407
Hsp92II CATG 6 cut(s) 134, 176, 632, 703, 817, 1102
KpnI GGTACC 1 cut(s) 26
Ksp22I TGATCA 1 cut(s) 532
KspAI GTTAAC 1 cut(s) 727
LmnI GCTCC 2 cut(s) 500, 810
Lsp1109I GCAGC 4 cut(s) 604, 829, 966, 1116
LweI GCATC 1 cut(s) 793
MaeI CTAG 7 cut(s) 435, 657, 663, 867, 876, 921, 1112
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 3 cut(s) 649, 767, 1054
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 2 cut(s) 879, 899
MlyI GAGTC 3 cut(s) 219, 657, 947
MroXI GAANNNNTTC 1 cut(s) 883
MseI TTAA 4 cut(s) 410, 726, 806, 1086
MslI CAYNNNNRTG 1 cut(s) 129
MspI CCGG 2 cut(s) 50, 832
MspR9I CCNGG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 450
MvnI CGCG 1 cut(s) 712
MwoI GCNNNNNNNGC 3 cut(s) 851, 1072, 1113
NlaIII CATG 6 cut(s) 134, 176, 632, 703, 817, 1102
NlaIV GGNNCC 4 cut(s) 24, 54, 502, 582
NmuCI GTSAC 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 883
PfeI GAWTC 2 cut(s) 7, 332
PkrI GCNGC 6 cut(s) 594, 844, 847, 951, 981, 1106
Ple19I CGATCG 1 cut(s) 222
PleI GAGTC 3 cut(s) 218, 656, 946
PpsI GAGTC 3 cut(s) 218, 656, 946
Psp1406I AACGTT 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 4 cut(s) 24, 54, 502, 582
PspPI GGNCC 1 cut(s) 178
PsuI RGATCY 1 cut(s) 64
PvuI CGATCG 1 cut(s) 222
RsaI GTAC 2 cut(s) 24, 874
RsaNI GTAC 2 cut(s) 23, 873
RseI CAYNNNNRTG 1 cut(s) 129
SalI GTCGAC 1 cut(s) 835
SaqAI TTAA 4 cut(s) 410, 726, 806, 1086
SatI GCNGC 6 cut(s) 593, 843, 846, 950, 980, 1105
Sau96I GGNCC 1 cut(s) 178
ScaI AGTACT 1 cut(s) 874
SchI GAGTC 3 cut(s) 219, 657, 947
ScrFI CCNGG 1 cut(s) 450
SduI GDGCHC 1 cut(s) 697
SfaNI GCATC 1 cut(s) 793
SmiMI CAYNNNNRTG 1 cut(s) 129
SpeI ACTAGT 2 cut(s) 656, 866
Sse9I AATT 2 cut(s) 879, 899
SsiI CCGC 6 cut(s) 127, 712, 845, 947, 950, 1066
SspI AATATT 1 cut(s) 927
SspMI CTAG 7 cut(s) 435, 657, 663, 867, 876, 921, 1112
StyD4I CCNGG 1 cut(s) 448
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 410
TaqI TCGA 5 cut(s) 201, 302, 492, 586, 836
TasI AATT 2 cut(s) 879, 899
TatI WGTACW 1 cut(s) 872
TauI GCSGC 2 cut(s) 848, 952
TfiI GAWTC 2 cut(s) 7, 332
Tru1I TTAA 4 cut(s) 410, 726, 806, 1086
Tru9I TTAA 4 cut(s) 410, 726, 806, 1086
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 4 cut(s) 592, 842, 979, 1104
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 5 cut(s) 569, 830, 1032, 1044, 1085
TspGWI ACGGA 2 cut(s) 744, 922
VneI GTGCAC 1 cut(s) 693
XapI RAATTY 1 cut(s) 899
XbaI TCTAGA 1 cut(s) 920
XmiI GTMKAC 1 cut(s) 836
XmnI GAANNNNTTC 1 cut(s) 883
XspI CTAG 7 cut(s) 435, 657, 663, 867, 876, 921, 1112
ZrmI AGTACT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.