pycom14g04720

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013027
Physical Location & Seq
Forward (+)
4454241 .. 4455118
878 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 507 bp
ATGCAAAGCGGGAAGAACGCGATGGAGTCGATAAAAGAAGCAGCTGCTAATATAGCAGCCTCTGCTATCTCTGGCATGGAAAAGACCAAACCCACCGTGCAAGAAAAGGTGGAGAAGATGAAATCCAGTGACCCAATTGAAAAGGACATGGCGGCCGGAAAGAAAGAGGAGAGAATAGTCGATGCCGAGATCAACAAACGTAATGCCATGGAGCAAAACGCCGCGGCTCGGCGGGATGTGGCAGGGGCTACCGGCTATACTGCGACCGGTGCTGGCACTGGAACCCACACGTATTCTACGACTGGGGCCACAGGGCAGCAGACTGGAACTCACCAGATATCCGCACTACCTGGCCACGGCACCGGTCAGCCTGCGGGCCAAGTGACGGAGGGTGCGGTGGGCGCGCACCCTGTTGGGATCAACACGGGTACGGGTCGGACCACAGCCCACAACACTCATGTTGGCGGGAATGTACCTAACTATGGGACTGGTGGGACCTATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

169

Amino Acids

17.06

Weight (kDa)

8.0

Isoelectric Point (pI)

22.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 8 - 77 1.4e-17 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 359
AccII CGCG 3 cut(s) 20, 224, 404
AciI CCGC 9 cut(s) 9, 152, 222, 224, 232, 342, 374, 395, 465
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 2 cut(s) 153, 352
AfaI GTAC 2 cut(s) 430, 474
AfiI CCNNNNNNNGG 4 cut(s) 228, 356, 385, 482
AflIII ACRYGT 1 cut(s) 288
AgeI ACCGGT 2 cut(s) 266, 362
AgsI TTSAA 1 cut(s) 140
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 1 cut(s) 44
AluI AGCT 1 cut(s) 44
AlwI GGATC 1 cut(s) 425
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 4 cut(s) 153, 306, 352, 376
ApeKI GCWGC 4 cut(s) 41, 44, 56, 316
AsiGI ACCGGT 2 cut(s) 266, 362
AspLEI GCGC 2 cut(s) 404, 406
AspS9I GGNCC 4 cut(s) 306, 376, 438, 495
AsuHPI GGTGA 1 cut(s) 323
AvaII GGWCC 2 cut(s) 438, 495
BalI TGGCCA 1 cut(s) 354
BanI GGYRCC 1 cut(s) 359
BbvI GCAGC 4 cut(s) 31, 53, 68, 328
BccI CCATC 1 cut(s) 16
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 351
BisI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
BlsI GCNGC 7 cut(s) 43, 46, 58, 154, 223, 226, 318
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 2 cut(s) 438, 495
BmgT120I GGNCC 4 cut(s) 306, 376, 438, 495
BmiI GGNNCC 4 cut(s) 283, 307, 361, 496
BmrFI CCNGG 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 312
BmsI GCATC 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 207, 222, 355
BsaWI WCCGGW 2 cut(s) 266, 362
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 356, 385, 482
Bse118I RCCGGY 3 cut(s) 251, 266, 362
Bse1I ACTGG 5 cut(s) 126, 283, 307, 328, 493
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 3 cut(s) 207, 222, 355
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 4 cut(s) 228, 356, 385, 482
BseNI ACTGG 5 cut(s) 126, 283, 307, 328, 493
BsePI GCGCGC 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 182
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 4 cut(s) 31, 53, 68, 328
Bsh1236I CGCG 3 cut(s) 20, 224, 404
Bsh1285I CGRYCG 2 cut(s) 156, 267
BshFI GGCC 4 cut(s) 155, 308, 354, 378
BshNI GGYRCC 1 cut(s) 359
BshTI ACCGGT 2 cut(s) 266, 362
BsiEI CGRYCG 2 cut(s) 156, 267
BsiSI CCGG 4 cut(s) 156, 252, 267, 363
BslFI GGGAC 1 cut(s) 499
BslI CCNNNNNNNGG 4 cut(s) 228, 356, 385, 482
BsmFI GGGAC 1 cut(s) 499
BsnI GGCC 4 cut(s) 155, 308, 354, 378
Bsp143I GATC 2 cut(s) 189, 417
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 9 cut(s) 9, 152, 222, 224, 232, 342, 374, 395, 465
BspANI GGCC 4 cut(s) 155, 308, 354, 378
BspFNI CGCG 3 cut(s) 20, 224, 404
BspLI GGNNCC 4 cut(s) 283, 307, 361, 496
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 359
BsrFI RCCGGY 3 cut(s) 251, 266, 362
BsrI ACTGG 5 cut(s) 126, 283, 307, 328, 493
BssAI RCCGGY 3 cut(s) 251, 266, 362
BssECI CCNNGG 3 cut(s) 207, 222, 355
BssHII GCGCGC 1 cut(s) 402
BssMI GATC 2 cut(s) 189, 417
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 291
BstC8I GCNNGC 4 cut(s) 274, 372, 376, 404
BstDSI CCRYGG 3 cut(s) 207, 222, 355
BstF5I GGATG 1 cut(s) 241
BstFNI CGCG 3 cut(s) 20, 224, 404
BstHHI GCGC 2 cut(s) 404, 406
BstKTI GATC 2 cut(s) 192, 420
BstMBI GATC 2 cut(s) 189, 417
BstMCI CGRYCG 2 cut(s) 156, 267
BstMWI GCNNNNNNNGC 4 cut(s) 53, 62, 269, 401
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstUI CGCG 3 cut(s) 20, 224, 404
BstV1I GCAGC 4 cut(s) 31, 53, 68, 328
BstZI CGGCCG 1 cut(s) 153
BsuRI GGCC 4 cut(s) 155, 308, 354, 378
BtgI CCRYGG 3 cut(s) 207, 222, 355
BtgZI GCGATG 1 cut(s) 35
BtsCI GGATG 1 cut(s) 241
BtsIMutI CAGTG 2 cut(s) 133, 276
Cac8I GCNNGC 4 cut(s) 274, 372, 376, 404
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 2 cut(s) 404, 406
Cfr10I RCCGGY 3 cut(s) 251, 266, 362
Cfr13I GGNCC 4 cut(s) 306, 376, 438, 495
Cfr42I CCGCGG 1 cut(s) 225
Csp6I GTAC 2 cut(s) 429, 473
CspAI ACCGGT 2 cut(s) 266, 362
CviAII CATG 4 cut(s) 76, 148, 208, 458
CviQI GTAC 2 cut(s) 429, 473
DpnI GATC 2 cut(s) 191, 419
DpnII GATC 2 cut(s) 189, 417
EaeI YGGCCR 2 cut(s) 153, 352
EagI CGGCCG 1 cut(s) 153
EclXI CGGCCG 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 207
Eco32I GATATC 1 cut(s) 339
Eco47I GGWCC 2 cut(s) 438, 495
Eco52I CGGCCG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 349
EcoRV GATATC 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 4 cut(s) 79, 151, 211, 461
FaiI YATR 8 cut(s) 53, 77, 149, 209, 258, 459, 483, 501
FaqI GGGAC 1 cut(s) 499
FatI CATG 4 cut(s) 75, 147, 207, 457
FauI CCCGC 4 cut(s) 2, 225, 367, 458
Fnu4HI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
GlaI GCGC 2 cut(s) 403, 405
GluI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
HaeIII GGCC 4 cut(s) 155, 308, 354, 378
HapII CCGG 4 cut(s) 156, 252, 267, 363
HhaI GCGC 2 cut(s) 404, 406
Hin1II CATG 4 cut(s) 79, 151, 211, 461
Hin6I GCGC 2 cut(s) 402, 404
HinP1I GCGC 2 cut(s) 402, 404
HinfI GANTC 1 cut(s) 26
HpaII CCGG 4 cut(s) 156, 252, 267, 363
HphI GGTGA 1 cut(s) 323
Hpy188I TCNGA 1 cut(s) 438
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 2 cut(s) 199, 290
HpyCH4V TGCA 2 cut(s) 4, 100
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 62, 269, 401
HpySE526I ACGT 2 cut(s) 199, 290
Hsp92II CATG 4 cut(s) 79, 151, 211, 461
HspAI GCGC 2 cut(s) 402, 404
KspI CCGCGG 1 cut(s) 225
Kzo9I GATC 2 cut(s) 189, 417
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 4 cut(s) 31, 53, 68, 328
LweI GCATC 1 cut(s) 172
MaeII ACGT 2 cut(s) 199, 290
MaeIII GTNAC 2 cut(s) 128, 382
MalI GATC 2 cut(s) 191, 419
MboI GATC 2 cut(s) 189, 417
MboII GAAGA 2 cut(s) 25, 127
MfeI CAATTG 1 cut(s) 135
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 2 cut(s) 135, 502
MluNI TGGCCA 1 cut(s) 354
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 3 cut(s) 70, 160, 382
Mox20I TGGCCA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 354
Msp20I TGGCCA 1 cut(s) 354
MspA1I CMGCKG 2 cut(s) 44, 224
MspI CCGG 4 cut(s) 156, 252, 267, 363
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 351
MvnI CGCG 3 cut(s) 20, 224, 404
MwoI GCNNNNNNNGC 4 cut(s) 53, 62, 269, 401
NcoI CCATGG 1 cut(s) 207
NdeII GATC 2 cut(s) 189, 417
NlaIII CATG 4 cut(s) 79, 151, 211, 461
NlaIV GGNNCC 4 cut(s) 283, 307, 361, 496
NmeAIII GCCGAG 2 cut(s) 208, 211
NmuCI GTSAC 2 cut(s) 128, 382
PauI GCGCGC 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PinAI ACCGGT 2 cut(s) 266, 362
PkrI GCNGC 7 cut(s) 43, 46, 58, 154, 223, 226, 318
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Ppu21I YACGTR 1 cut(s) 291
PpuMI RGGWCCY 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 4 cut(s) 283, 307, 361, 496
PspPI GGNCC 4 cut(s) 306, 376, 438, 495
PspPPI RGGWCCY 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 62
PteI GCGCGC 1 cut(s) 402
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 2 cut(s) 430, 474
RsaNI GTAC 2 cut(s) 429, 473
SacII CCGCGG 1 cut(s) 225
SatI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
Sau3AI GATC 2 cut(s) 189, 417
Sau96I GGNCC 4 cut(s) 306, 376, 438, 495
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 351
SetI ASST 7 cut(s) 46, 111, 202, 293, 352, 478, 500
SfaNI GCATC 1 cut(s) 172
Sfr303I CCGCGG 1 cut(s) 225
SgrBI CCGCGG 1 cut(s) 225
SinI GGWCC 2 cut(s) 438, 495
Sse9I AATT 2 cut(s) 135, 502
SsiI CCGC 9 cut(s) 9, 152, 222, 224, 232, 342, 374, 395, 465
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 2 cut(s) 202, 293
TaqI TCGA 2 cut(s) 29, 180
TasI AATT 2 cut(s) 135, 502
TauI GCSGC 3 cut(s) 155, 224, 227
TscAI CASTG 2 cut(s) 133, 283
TseFI GTSAC 2 cut(s) 128, 382
TseI GCWGC 4 cut(s) 41, 44, 56, 316
Tsp45I GTSAC 2 cut(s) 128, 382
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 133, 283
VpaK11BI GGWCC 2 cut(s) 438, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.