pycom14g06920

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006449
Physical Location & Seq
Forward (+)
6883315 .. 6884709
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGGCTAAATCAAGTTCATCAGTTGGTAGAGCCAGAGGCCCTTCTGTGATTTCCATGATACTTGTTCTTGGGCAGCTGATGGCCAGCTTCGAAAGAACAGGCGCCCAAATTGGTGTATGTTATGGAATGCTGGGAGACTTACCACCCCCAGCAGAAGTTATTGCTCTCTACAAGCAAAATAACATCCGAAGAATGCGACTATATGATCCGAACCCAGCAGCTCTAGCAGCCCTCAGAGGCTCTGACATTGAGCTCATATTAGACATACCAAAGGACCAACTTCAAAACATTGCTTCAAGTCAAGCCAATGCAAATACATGGGTCCAAAACAATGTCAGAAACTATGCCAATGTAAGATTCAAATACATTGCCGTAGGAAACGAAATCAAGCCCTCGGACTCGTCTGCTCAATTTCTGGTCCCGGCCATGCGGAACATTCAAAATGCGATTTCCAGTGCCGGTCTTGGAAACCAAATCAAAGTTTCCACCGCCATAGACACCGGTGTGCTTGGAAATTCCTTTCCTCCATCAAAAGGAGAATTTAAGGGTGACTATAGCCCAATTTTGAATCCTGTTGTCCGGTTCCTGGTGGACAACAAATCTCCGCTACTCGTTAATTTGTATCCTTATTTTAGTTATATTGGCAACACTCGTGACATTCGTCTAGATTATGCTCTTTTCACAGCTCCGTCAGTTGTAGTACAAGATGGCCAACGTGGTTATCGTAATCTTTTCGATGCCATTTTGGATGCTGTTTACGCTGCGCTTGACAAGGTCGGTGGAGGATCTTTGGAAATTGTTATATCGGAGAGTGGTTGGCCGACGGCTGGTGGGACGGCAACAACAGTTGATAATGCGAGGACTTATAACTCGAATTTGATTCAACATGTCAAGGTAGGGACTCCAAGGAAGCCTGGAAAGCCCATTGAAACTTACATCTTTGCCATGTTTGATGAGAATAGAAAGACCCCAGAGTTTGAGAAACATTGGGGGCTATTCTCCCCTAACAAACAGCCTAAATACCCAATCAATTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

347

Amino Acids

37.87

Weight (kDa)

9.3

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 37 - 345 5.3e-119 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
AccB1I GGYRCC 1 cut(s) 101
AciI CCGC 3 cut(s) 430, 489, 605
AclWI GGATC 2 cut(s) 200, 793
AcoI YGGCCR 4 cut(s) 81, 423, 709, 818
AcsI RAATTY 3 cut(s) 514, 539, 874
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 3 cut(s) 533, 586, 827
AflIII ACRYGT 1 cut(s) 886
AgeI ACCGGT 1 cut(s) 500
AgsI TTSAA 8 cut(s) 284, 297, 361, 440, 568, 884, 929, 1036
AjnI CCWGG 2 cut(s) 585, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 5 cut(s) 76, 87, 221, 253, 686
AluI AGCT 5 cut(s) 76, 87, 221, 253, 686
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 2 cut(s) 200, 793
AoxI GGCC 5 cut(s) 37, 81, 423, 709, 818
ApeKI GCWGC 4 cut(s) 73, 218, 227, 761
ApoI RAATTY 3 cut(s) 514, 539, 874
AsiGI ACCGGT 1 cut(s) 500
AspLEI GCGC 2 cut(s) 104, 766
AspS9I GGNCC 4 cut(s) 38, 274, 322, 418
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 1 cut(s) 560
AsuII TTCGAA 1 cut(s) 90
AvaII GGWCC 3 cut(s) 274, 322, 418
BalI TGGCCA 2 cut(s) 83, 711
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 255
BauI CACGAG 1 cut(s) 651
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 4 cut(s) 85, 230, 239, 748
BccI CCATC 3 cut(s) 73, 535, 701
BceAI ACGGC 3 cut(s) 356, 840, 852
BciT130I CCWGG 2 cut(s) 587, 915
BciVI GTATCC 1 cut(s) 633
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 2 cut(s) 224, 665
BfmI CTRYAG 1 cut(s) 553
BfoI RGCGCY 1 cut(s) 105
BfuI GTATCC 1 cut(s) 633
BisI GCNGC 4 cut(s) 74, 219, 228, 762
BlsI GCNGC 4 cut(s) 75, 220, 229, 763
Bme1390I CCNGG 3 cut(s) 422, 587, 915
Bme18I GGWCC 3 cut(s) 274, 322, 418
BmgT120I GGNCC 4 cut(s) 38, 274, 322, 418
BmiI GGNNCC 4 cut(s) 103, 323, 420, 584
BmrFI CCNGG 3 cut(s) 422, 587, 915
BmsI GCATC 2 cut(s) 727, 739
BoxI GACNNNNGTC 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 393, 905
BsaWI WCCGGW 2 cut(s) 500, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 533, 586, 827
Bse118I RCCGGY 2 cut(s) 458, 500
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 2 cut(s) 288, 366
BseBI CCWGG 2 cut(s) 587, 915
BseDI CCNNGG 2 cut(s) 393, 905
BseGI GGATG 2 cut(s) 183, 754
BseLI CCNNNNNNNGG 3 cut(s) 533, 586, 827
BseMI GCAATG 2 cut(s) 288, 366
BseMII CTCAG 1 cut(s) 247
BseNI ACTGG 1 cut(s) 453
BseXI GCAGC 4 cut(s) 85, 230, 239, 748
BseYI CCCAGC 3 cut(s) 130, 148, 214
BshFI GGCC 5 cut(s) 39, 83, 425, 711, 820
BshNI GGYRCC 1 cut(s) 101
BshTI ACCGGT 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 4 cut(s) 422, 459, 501, 580
BslFI GGGAC 3 cut(s) 404, 847, 913
BslI CCNNNNNNNGG 3 cut(s) 533, 586, 827
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 3 cut(s) 404, 847, 913
BsmI GAATGC 2 cut(s) 132, 198
BsnI GGCC 5 cut(s) 39, 83, 425, 711, 820
Bsp119I TTCGAA 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 205, 785
BspACI CCGC 3 cut(s) 430, 489, 605
BspANI GGCC 5 cut(s) 39, 83, 425, 711, 820
BspCNI CTCAG 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 103, 323, 420, 584
BspPI GGATC 2 cut(s) 200, 793
BspT104I TTCGAA 1 cut(s) 90
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 2 cut(s) 288, 366
BsrFI RCCGGY 2 cut(s) 458, 500
BsrI ACTGG 1 cut(s) 453
BssAI RCCGGY 2 cut(s) 458, 500
BssECI CCNNGG 2 cut(s) 393, 905
BssMI GATC 2 cut(s) 205, 785
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 651
BssT1I CCWWGG 1 cut(s) 905
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 2 cut(s) 587, 915
Bst4CI ACNGT 1 cut(s) 847
BstACI GRCGYC 1 cut(s) 102
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 183, 754
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 2 cut(s) 104, 766
BstKTI GATC 2 cut(s) 208, 788
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 2 cut(s) 205, 785
BstMWI GCNNNNNNNGC 4 cut(s) 224, 227, 758, 919
BstNI CCWGG 2 cut(s) 587, 915
BstNSI RCATGY 1 cut(s) 890
BstPAI GACNNNNGTC 1 cut(s) 660
BstSCI CCNGG 3 cut(s) 420, 585, 913
BstSFI CTRYAG 1 cut(s) 553
BstV1I GCAGC 4 cut(s) 85, 230, 239, 748
BstX2I RGATCY 1 cut(s) 785
BstYI RGATCY 1 cut(s) 785
BsuI GTATCC 1 cut(s) 633
BsuRI GGCC 5 cut(s) 39, 83, 425, 711, 820
BtsCI GGATG 2 cut(s) 183, 754
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 85
CfoI GCGC 2 cut(s) 104, 766
Cfr10I RCCGGY 2 cut(s) 458, 500
Cfr13I GGNCC 4 cut(s) 38, 274, 322, 418
Csp6I GTAC 1 cut(s) 701
CspAI ACCGGT 1 cut(s) 500
CspCI CAANNNNNGTGG 2 cut(s) 760, 795
CviAII CATG 5 cut(s) 55, 318, 427, 887, 946
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 1 cut(s) 233
DinI GGCGCC 1 cut(s) 103
DpnI GATC 2 cut(s) 207, 787
DpnII GATC 2 cut(s) 205, 785
EaeI YGGCCR 4 cut(s) 81, 423, 709, 818
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 905
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 3 cut(s) 274, 322, 418
Eco53kI GAGCTC 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 38
EcoRII CCWGG 2 cut(s) 585, 913
EcoT14I CCWWGG 1 cut(s) 905
EcoT38I GRGCYC 1 cut(s) 255
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 905
FaeI CATG 5 cut(s) 58, 321, 430, 890, 949
FaqI GGGAC 3 cut(s) 404, 847, 913
FatI CATG 5 cut(s) 54, 317, 426, 886, 945
Fnu4HI GCNGC 4 cut(s) 74, 219, 228, 762
FokI GGATG 2 cut(s) 170, 761
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 4 cut(s) 74, 219, 228, 762
FspBI CTAG 2 cut(s) 224, 665
GlaI GCGC 2 cut(s) 103, 765
GluI GCNGC 4 cut(s) 74, 219, 228, 762
GsaI CCCAGC 3 cut(s) 134, 152, 218
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 5 cut(s) 39, 83, 425, 711, 820
HapII CCGG 4 cut(s) 422, 459, 501, 580
HhaI GCGC 2 cut(s) 104, 766
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 5 cut(s) 58, 321, 430, 890, 949
Hin6I GCGC 2 cut(s) 102, 764
HinP1I GCGC 2 cut(s) 102, 764
HinfI GANTC 5 cut(s) 357, 398, 568, 880, 901
HpaII CCGG 4 cut(s) 422, 459, 501, 580
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 2 cut(s) 592, 757
Hpy188I TCNGA 7 cut(s) 188, 210, 236, 244, 338, 397, 808
Hpy188III TCNNGA 2 cut(s) 653, 665
Hpy8I GTNNAC 2 cut(s) 592, 757
Hpy99I CGWCG 1 cut(s) 826
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 1 cut(s) 847
HpyCH4IV ACGT 1 cut(s) 715
HpyCH4V TGCA 1 cut(s) 311
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 227, 758, 919
HpyF3I CTNAG 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 715
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 5 cut(s) 58, 321, 430, 890, 949
HspAI GCGC 2 cut(s) 102, 764
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 205, 785
LmnI GCTCC 1 cut(s) 691
Lsp1109I GCAGC 4 cut(s) 85, 230, 239, 748
LweI GCATC 2 cut(s) 727, 739
MaeI CTAG 2 cut(s) 224, 665
MaeII ACGT 1 cut(s) 715
MaeIII GTNAC 2 cut(s) 548, 653
MalI GATC 2 cut(s) 207, 787
MboI GATC 2 cut(s) 205, 785
MboII GAAGA 1 cut(s) 201
MflI RGATCY 1 cut(s) 785
MhlI GDGCHC 1 cut(s) 255
MlsI TGGCCA 2 cut(s) 83, 711
MluCI AATT 9 cut(s) 108, 410, 514, 539, 561, 616, 795, 874, 1030
MluNI TGGCCA 2 cut(s) 83, 711
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 2 cut(s) 392, 895
MnlI CCTC 7 cut(s) 29, 230, 242, 403, 534, 776, 852
Mox20I TGGCCA 2 cut(s) 83, 711
MscI TGGCCA 2 cut(s) 83, 711
MseI TTAA 2 cut(s) 543, 615
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 2 cut(s) 83, 711
MspA1I CMGCKG 1 cut(s) 76
MspI CCGG 4 cut(s) 422, 459, 501, 580
MspR9I CCNGG 3 cut(s) 422, 587, 915
Mva1269I GAATGC 2 cut(s) 132, 198
MvaI CCWGG 2 cut(s) 587, 915
MwoI GCNNNNNNNGC 4 cut(s) 224, 227, 758, 919
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 1 cut(s) 422
NdeII GATC 2 cut(s) 205, 785
NlaIII CATG 5 cut(s) 58, 321, 430, 890, 949
NlaIV GGNNCC 4 cut(s) 103, 323, 420, 584
NmuCI GTSAC 2 cut(s) 548, 653
NspI RCATGY 1 cut(s) 890
NspV TTCGAA 1 cut(s) 90
OliI CACNNNNGTG 1 cut(s) 503
PciI ACATGT 1 cut(s) 886
PcsI WCGNNNNNNNCGW 2 cut(s) 658, 721
PctI GAATGC 2 cut(s) 132, 198
PfeI GAWTC 3 cut(s) 357, 568, 880
PflFI GACNNNGTC 1 cut(s) 773
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 4 cut(s) 75, 220, 229, 763
PleI GAGTC 2 cut(s) 392, 895
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 2 cut(s) 392, 895
PscI ACATGT 1 cut(s) 886
PshAI GACNNNNGTC 1 cut(s) 660
PsiI TTATAA 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 585, 913
PspFI CCCAGC 3 cut(s) 130, 148, 214
PspGI CCWGG 2 cut(s) 585, 913
PspN4I GGNNCC 4 cut(s) 103, 323, 420, 584
PspPI GGNCC 4 cut(s) 38, 274, 322, 418
PsuI RGATCY 1 cut(s) 785
PsyI GACNNNGTC 1 cut(s) 773
PvuII CAGCTG 1 cut(s) 76
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 543, 615
SatI GCNGC 4 cut(s) 74, 219, 228, 762
Sau3AI GATC 2 cut(s) 205, 785
Sau96I GGNCC 4 cut(s) 38, 274, 322, 418
SchI GAGTC 2 cut(s) 392, 895
ScrFI CCNGG 3 cut(s) 422, 587, 915
SduI GDGCHC 1 cut(s) 255
SetI ASST 8 cut(s) 78, 89, 223, 255, 688, 718, 777, 897
SfaNI GCATC 2 cut(s) 727, 739
SfcI CTRYAG 1 cut(s) 553
SfoI GGCGCC 1 cut(s) 103
SfuI TTCGAA 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 500
SinI GGWCC 3 cut(s) 274, 322, 418
SmiMI CAYNNNNRTG 1 cut(s) 503
Sse9I AATT 9 cut(s) 108, 410, 514, 539, 561, 616, 795, 874, 1030
SsiI CCGC 3 cut(s) 430, 489, 605
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 224, 665
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 3 cut(s) 420, 585, 913
StyI CCWWGG 1 cut(s) 905
TaaI ACNGT 1 cut(s) 847
TaiI ACGT 1 cut(s) 718
TaqI TCGA 3 cut(s) 90, 735, 872
TasI AATT 9 cut(s) 108, 410, 514, 539, 561, 616, 795, 874, 1030
TatI WGTACW 1 cut(s) 700
TfiI GAWTC 3 cut(s) 357, 568, 880
Tru1I TTAA 2 cut(s) 543, 615
Tru9I TTAA 2 cut(s) 543, 615
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 2 cut(s) 548, 653
TseI GCWGC 4 cut(s) 73, 218, 227, 761
Tsp45I GTSAC 2 cut(s) 548, 653
TspDTI ATGAA 1 cut(s) 6
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 1 cut(s) 460
Tth111I GACNNNGTC 1 cut(s) 773
VpaK11BI GGWCC 3 cut(s) 274, 322, 418
XapI RAATTY 3 cut(s) 514, 539, 874
XbaI TCTAGA 1 cut(s) 664
XceI RCATGY 1 cut(s) 890
XspI CTAG 2 cut(s) 224, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.