pycom14g06930

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006449
Physical Location & Seq
Forward (+)
6887855 .. 6889200
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGGCTAAATCAAGTTCATCAGTTGGTAGAGCCACAGGCCCTTCTGTGATTTCCATGATACTTGTTCTTGGGCAGCTGATGGCCAGCTTCGAAACAACAGGCGCCCAAATTGGTGTATGTTATGGAATGTTGGGAGACTTACCACCCCCAGCAGAAGTTATTGCTCTCTACAAGAAAAATAACATCCGAAGAATGCGACTATATGATCCGAACCCAGCAGCTCTAGCAGCCCTCAGAGGCTCTGGCATTGAGCTCATATTAGACATACCAAAGGACCAACTTCAAAATATTGCTTCAAGTCAAGCCAATGCAAATACATGGGTCCAAAACAATGTCAGAAAGTATGCCAATGTAAGATTCAAATACATTGCCGTAGGAAACGAAGTCAAGCCCTCGGACTCGTCTGCTCAATTTCTGGTCCCGGCCATGCGGAACATTCAAAATGCGATTTCCAGTGCCGGTCTTGGAAACCAAATCAAAGTTTCCACCGCCATAGACACCGGTGTGCTTGGAAATTCCTTTCCTCCATCAAAAGGAGAATTTAAGGGTGACTATAGCCCAATTTTGAATCCTGTTGTCCGGTTCCTGGTGGACAACAAATCTCCGCTACTCGTTAATTTGTATCCTTATTTTAGTTATATTGGCAACCCTCGTGACATTCGTCTAGATTATGCTCTTTTCACAGCTCCGTCAGTTGTAGTACAAGATGGCCAACGTGGTTATCGTAATCTTTTCGATGCCATTTTGGATGCTGTTTACGCTGCGCTTGACAAGGTCGGTGGAGGATCTTTGGAAATTGTTATATCGGAGAGTGGTTGGCCGACAGCTGGTGGGACGGCAACAACAGTTGATAATGCGAGGACTTATAACTCGAATTTGATTCAACATGTGAAGGGAGGGACTCCAAGGAAGCCTGGAAAACCCATTGAAACTTACATCTTTGCCATGTTTGATGAGAATAGAAAGACCCCAGAGTTTGAGAAACATTGGGGGCTATTCTCCCCTAACAAACAGCCTAAATACCCAATCAATTTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

347

Amino Acids

37.65

Weight (kDa)

9.35

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 37 - 345 4.7e-118 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
AccB1I GGYRCC 1 cut(s) 101
AciI CCGC 3 cut(s) 430, 489, 605
AclWI GGATC 2 cut(s) 200, 793
AcoI YGGCCR 4 cut(s) 81, 423, 709, 818
AcsI RAATTY 3 cut(s) 514, 539, 874
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 3 cut(s) 533, 586, 827
AflIII ACRYGT 1 cut(s) 886
AgeI ACCGGT 1 cut(s) 500
AgsI TTSAA 7 cut(s) 284, 297, 361, 440, 568, 884, 929
AjnI CCWGG 2 cut(s) 585, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 6 cut(s) 76, 87, 221, 253, 686, 827
AluI AGCT 6 cut(s) 76, 87, 221, 253, 686, 827
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 2 cut(s) 200, 793
AoxI GGCC 5 cut(s) 37, 81, 423, 709, 818
ApeKI GCWGC 4 cut(s) 73, 218, 227, 761
ApoI RAATTY 3 cut(s) 514, 539, 874
AsiGI ACCGGT 1 cut(s) 500
AspLEI GCGC 2 cut(s) 104, 766
AspS9I GGNCC 4 cut(s) 38, 274, 322, 418
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 1 cut(s) 560
AsuII TTCGAA 1 cut(s) 90
AvaII GGWCC 3 cut(s) 274, 322, 418
BalI TGGCCA 2 cut(s) 83, 711
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 255
BauI CACGAG 1 cut(s) 651
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 4 cut(s) 85, 230, 239, 748
BccI CCATC 3 cut(s) 73, 535, 701
BceAI ACGGC 2 cut(s) 356, 852
BciT130I CCWGG 2 cut(s) 587, 915
BciVI GTATCC 1 cut(s) 633
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 2 cut(s) 224, 665
BfmI CTRYAG 1 cut(s) 553
BfoI RGCGCY 1 cut(s) 105
BfuI GTATCC 1 cut(s) 633
BisI GCNGC 4 cut(s) 74, 219, 228, 762
BlsI GCNGC 4 cut(s) 75, 220, 229, 763
Bme1390I CCNGG 3 cut(s) 422, 587, 915
Bme18I GGWCC 3 cut(s) 274, 322, 418
BmgT120I GGNCC 4 cut(s) 38, 274, 322, 418
BmiI GGNNCC 4 cut(s) 103, 323, 420, 584
BmrFI CCNGG 3 cut(s) 422, 587, 915
BmsI GCATC 2 cut(s) 727, 739
BoxI GACNNNNGTC 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 393, 905
BsaWI WCCGGW 2 cut(s) 500, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 533, 586, 827
Bse118I RCCGGY 2 cut(s) 458, 500
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 1 cut(s) 366
BseBI CCWGG 2 cut(s) 587, 915
BseDI CCNNGG 2 cut(s) 393, 905
BseGI GGATG 2 cut(s) 183, 754
BseLI CCNNNNNNNGG 3 cut(s) 533, 586, 827
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 247
BseNI ACTGG 1 cut(s) 453
BseXI GCAGC 4 cut(s) 85, 230, 239, 748
BseYI CCCAGC 2 cut(s) 148, 214
BshFI GGCC 5 cut(s) 39, 83, 425, 711, 820
BshNI GGYRCC 1 cut(s) 101
BshTI ACCGGT 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 4 cut(s) 422, 459, 501, 580
BslFI GGGAC 3 cut(s) 404, 847, 913
BslI CCNNNNNNNGG 3 cut(s) 533, 586, 827
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 3 cut(s) 404, 847, 913
BsmI GAATGC 1 cut(s) 198
BsnI GGCC 5 cut(s) 39, 83, 425, 711, 820
Bsp119I TTCGAA 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 205, 785
BspACI CCGC 3 cut(s) 430, 489, 605
BspANI GGCC 5 cut(s) 39, 83, 425, 711, 820
BspCNI CTCAG 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 103, 323, 420, 584
BspPI GGATC 2 cut(s) 200, 793
BspT104I TTCGAA 1 cut(s) 90
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 2 cut(s) 458, 500
BsrI ACTGG 1 cut(s) 453
BssAI RCCGGY 2 cut(s) 458, 500
BssECI CCNNGG 2 cut(s) 393, 905
BssMI GATC 2 cut(s) 205, 785
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 651
BssT1I CCWWGG 1 cut(s) 905
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 2 cut(s) 587, 915
Bst4CI ACNGT 1 cut(s) 847
BstACI GRCGYC 1 cut(s) 102
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 183, 754
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 2 cut(s) 104, 766
BstKTI GATC 2 cut(s) 208, 788
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 2 cut(s) 205, 785
BstMWI GCNNNNNNNGC 3 cut(s) 224, 227, 758
BstNI CCWGG 2 cut(s) 587, 915
BstNSI RCATGY 1 cut(s) 890
BstPAI GACNNNNGTC 1 cut(s) 660
BstSCI CCNGG 3 cut(s) 420, 585, 913
BstSFI CTRYAG 1 cut(s) 553
BstV1I GCAGC 4 cut(s) 85, 230, 239, 748
BstX2I RGATCY 1 cut(s) 785
BstYI RGATCY 1 cut(s) 785
BsuI GTATCC 1 cut(s) 633
BsuRI GGCC 5 cut(s) 39, 83, 425, 711, 820
BtsCI GGATG 2 cut(s) 183, 754
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 85
CfoI GCGC 2 cut(s) 104, 766
Cfr10I RCCGGY 2 cut(s) 458, 500
Cfr13I GGNCC 4 cut(s) 38, 274, 322, 418
Csp6I GTAC 1 cut(s) 701
CspAI ACCGGT 1 cut(s) 500
CspCI CAANNNNNGTGG 2 cut(s) 760, 795
CviAII CATG 5 cut(s) 55, 318, 427, 887, 946
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 1 cut(s) 233
DinI GGCGCC 1 cut(s) 103
DpnI GATC 2 cut(s) 207, 787
DpnII GATC 2 cut(s) 205, 785
EaeI YGGCCR 4 cut(s) 81, 423, 709, 818
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 905
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 3 cut(s) 274, 322, 418
Eco53kI GAGCTC 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 38
EcoRII CCWGG 2 cut(s) 585, 913
EcoT14I CCWWGG 1 cut(s) 905
EcoT38I GRGCYC 1 cut(s) 255
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 905
FaeI CATG 5 cut(s) 58, 321, 430, 890, 949
FaqI GGGAC 3 cut(s) 404, 847, 913
FatI CATG 5 cut(s) 54, 317, 426, 886, 945
Fnu4HI GCNGC 4 cut(s) 74, 219, 228, 762
FokI GGATG 2 cut(s) 170, 761
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 4 cut(s) 74, 219, 228, 762
FspBI CTAG 2 cut(s) 224, 665
GlaI GCGC 2 cut(s) 103, 765
GluI GCNGC 4 cut(s) 74, 219, 228, 762
GsaI CCCAGC 2 cut(s) 152, 218
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 5 cut(s) 39, 83, 425, 711, 820
HapII CCGG 4 cut(s) 422, 459, 501, 580
HhaI GCGC 2 cut(s) 104, 766
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 5 cut(s) 58, 321, 430, 890, 949
Hin6I GCGC 2 cut(s) 102, 764
HinP1I GCGC 2 cut(s) 102, 764
HinfI GANTC 5 cut(s) 357, 398, 568, 880, 901
HpaII CCGG 4 cut(s) 422, 459, 501, 580
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 2 cut(s) 592, 757
Hpy188I TCNGA 6 cut(s) 188, 210, 236, 338, 397, 808
Hpy188III TCNNGA 2 cut(s) 653, 665
Hpy8I GTNNAC 2 cut(s) 592, 757
HpyAV CCTTC 2 cut(s) 51, 886
HpyCH4III ACNGT 1 cut(s) 847
HpyCH4IV ACGT 1 cut(s) 715
HpyCH4V TGCA 1 cut(s) 311
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 227, 758
HpyF3I CTNAG 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 715
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 5 cut(s) 58, 321, 430, 890, 949
HspAI GCGC 2 cut(s) 102, 764
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 205, 785
LmnI GCTCC 1 cut(s) 691
Lsp1109I GCAGC 4 cut(s) 85, 230, 239, 748
LweI GCATC 2 cut(s) 727, 739
MaeI CTAG 2 cut(s) 224, 665
MaeII ACGT 1 cut(s) 715
MaeIII GTNAC 2 cut(s) 548, 653
MalI GATC 2 cut(s) 207, 787
MboI GATC 2 cut(s) 205, 785
MboII GAAGA 1 cut(s) 201
MflI RGATCY 1 cut(s) 785
MhlI GDGCHC 1 cut(s) 255
MlsI TGGCCA 2 cut(s) 83, 711
MluCI AATT 9 cut(s) 108, 410, 514, 539, 561, 616, 795, 874, 1030
MluNI TGGCCA 2 cut(s) 83, 711
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 2 cut(s) 392, 895
MnlI CCTC 8 cut(s) 230, 242, 403, 534, 660, 776, 852, 890
Mox20I TGGCCA 2 cut(s) 83, 711
MscI TGGCCA 2 cut(s) 83, 711
MseI TTAA 3 cut(s) 543, 615, 1035
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 2 cut(s) 83, 711
MspA1I CMGCKG 2 cut(s) 76, 827
MspI CCGG 4 cut(s) 422, 459, 501, 580
MspR9I CCNGG 3 cut(s) 422, 587, 915
Mva1269I GAATGC 1 cut(s) 198
MvaI CCWGG 2 cut(s) 587, 915
MwoI GCNNNNNNNGC 3 cut(s) 224, 227, 758
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 1 cut(s) 422
NdeII GATC 2 cut(s) 205, 785
NlaIII CATG 5 cut(s) 58, 321, 430, 890, 949
NlaIV GGNNCC 4 cut(s) 103, 323, 420, 584
NmuCI GTSAC 2 cut(s) 548, 653
NspI RCATGY 1 cut(s) 890
NspV TTCGAA 1 cut(s) 90
OliI CACNNNNGTG 1 cut(s) 503
PciI ACATGT 1 cut(s) 886
PcsI WCGNNNNNNNCGW 2 cut(s) 658, 721
PctI GAATGC 1 cut(s) 198
PfeI GAWTC 3 cut(s) 357, 568, 880
PflFI GACNNNGTC 1 cut(s) 773
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 4 cut(s) 75, 220, 229, 763
PleI GAGTC 2 cut(s) 392, 895
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 2 cut(s) 392, 895
PscI ACATGT 1 cut(s) 886
PshAI GACNNNNGTC 1 cut(s) 660
PsiI TTATAA 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 585, 913
PspFI CCCAGC 2 cut(s) 148, 214
PspGI CCWGG 2 cut(s) 585, 913
PspN4I GGNNCC 4 cut(s) 103, 323, 420, 584
PspPI GGNCC 4 cut(s) 38, 274, 322, 418
PsuI RGATCY 1 cut(s) 785
PsyI GACNNNGTC 1 cut(s) 773
PvuII CAGCTG 2 cut(s) 76, 827
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 255
SaqAI TTAA 3 cut(s) 543, 615, 1035
SatI GCNGC 4 cut(s) 74, 219, 228, 762
Sau3AI GATC 2 cut(s) 205, 785
Sau96I GGNCC 4 cut(s) 38, 274, 322, 418
SchI GAGTC 2 cut(s) 392, 895
ScrFI CCNGG 3 cut(s) 422, 587, 915
SduI GDGCHC 1 cut(s) 255
SetI ASST 8 cut(s) 78, 89, 223, 255, 688, 718, 777, 829
SfaNI GCATC 2 cut(s) 727, 739
SfcI CTRYAG 1 cut(s) 553
SfoI GGCGCC 1 cut(s) 103
SfuI TTCGAA 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 500
SinI GGWCC 3 cut(s) 274, 322, 418
SmiMI CAYNNNNRTG 1 cut(s) 503
Sse9I AATT 9 cut(s) 108, 410, 514, 539, 561, 616, 795, 874, 1030
SsiI CCGC 3 cut(s) 430, 489, 605
SspDI GGCGCC 1 cut(s) 101
SspI AATATT 1 cut(s) 289
SspMI CTAG 2 cut(s) 224, 665
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 3 cut(s) 420, 585, 913
StyI CCWWGG 1 cut(s) 905
TaaI ACNGT 1 cut(s) 847
TaiI ACGT 1 cut(s) 718
TaqI TCGA 3 cut(s) 90, 735, 872
TasI AATT 9 cut(s) 108, 410, 514, 539, 561, 616, 795, 874, 1030
TatI WGTACW 1 cut(s) 700
TfiI GAWTC 3 cut(s) 357, 568, 880
Tru1I TTAA 3 cut(s) 543, 615, 1035
Tru9I TTAA 3 cut(s) 543, 615, 1035
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 2 cut(s) 548, 653
TseI GCWGC 4 cut(s) 73, 218, 227, 761
Tsp45I GTSAC 2 cut(s) 548, 653
TspDTI ATGAA 1 cut(s) 6
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 1 cut(s) 460
Tth111I GACNNNGTC 1 cut(s) 773
VpaK11BI GGWCC 3 cut(s) 274, 322, 418
XapI RAATTY 3 cut(s) 514, 539, 874
XbaI TCTAGA 1 cut(s) 664
XceI RCATGY 1 cut(s) 890
XspI CTAG 2 cut(s) 224, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.