pycom14g09580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008872
Physical Location & Seq
Forward (+)
11547192 .. 11553519
6328 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4326 bp
ATGTTCAGGCTACACAAGAACAGGCCTGCTAAATCAGGGGAGAGGGTGGATTTCAAGTTCTCCCACTTTAAGGCCCTCCAGGTTCCCAGAGGGTGGGACAAGCTGTTTGTATCAATTGTGTCTGTAGAAACTGGGAAACCAATTGTTAAATCAAGCAAAGCAGTGGTGCGCAACGGAAACTGTCAGTGGACTGAGACCCTTTGCGAATCTATATGGATTTCCCAAGACGAGTCTTCGAAGGAGATGGAAGATTATTTCATCAAGCTTGTCGTTTCCATGGGTTCGGCAAGATCCGGCATCCTTGGAGAGGTTTCAGTCAATATGTCAGATTACATAAGTTCAACCTCTTCTGCTCCAGTTTCTCTTCCATTGAAAAAGTGCAGTTTTGGAACAATTTTACAGGTAAAAATTAACTGCCTGACACCAAGAAAAAAGCTCAGTGATGAAGAGTCAAAAGAGACGAGCTTTCATTCAGAAGAAGTAATTGAAAATGGTCATGATGCGGACAGCAAAAAAGATTTTGGCTTGATCTCAAATCCCGGAGAGCCTGGGAGTCAAGATAGTAGTTTCTCTGCATCAGGTTCACATCGCAGCTATGACTCGGCAGAGGGCTCCATAAGAAAGGATAATATGTCCATGAGAAGCAACCTTAGTGTTGAAGGCAACCACCAGATAGGAAGACAAGATTCAACCACCTCCCTAATTAGTACATCTCATGGAATTTTTCCTGCTGATATTCATTCTCCGTCAGACCATTCATCATTCAATTCCAGGATTAATGGTTCAAGAATTCAGTCTCAGAATTCCCGGAAAGATTTTACAGAATCTTCTTTAAGTACCACTGATTCTTCTAAAAACCTTCTGGATGCGGCAGAAGTTACGATTGAAGAGTTACATGCGGAAGCTAAGATGTGGGAAAGGAATGCCAGGAAGCTAATGCTTGATTTGGATATAATGAGGGCAGAGTTCTCTGATCGATCCAAAAAGCAGGCAAATATGAATGTGGAGCTTTCAACAGCATATGCAGAACGCGATGGCTTGAAGAAAGAAGTTGAACACTTGCATTTGTTGCTTGAGAATGCAATGGTAAAACAAACAGCCAGTGAGGATTTAACTTCTCAGGATGGAGGAACACTCGAGATTGAGAAGGCATTCCAAGATGAACTGAACTTTCAAAAAGAATCCGCAGCCAATTTGTCCCTGCAGTTGGAGAGAAGTCAAGAATTAAATATTGAGCTGGTTTCAGTTCTCCAGGAGCTGGAAGAGACAATTGAAAAACAAAAAGTGGAATTGGAGAATCTTTCAGAACTACAGTCGACATTTGGTGATATGAAAAACTTGATCAATTTAACTACTGAGGAAAACAGAAATTTAAAGCTTCAGCTGCAACAATTGCAGGAATCAGAGAACAAGTTGCAAGTTGTGGTGCAACAAGTGGAACAAACCGTGGAAGAGAAAAATCATGAGATAGAGAATGGATCGAGTCTGAACGAACACTCCCTTTTGGATATTGAGACAGAATACAAAAGTAAACTTCTTTTGAAGGAACAAGAAATTGTCAAACTGAAAGCAAAGGTGTCCGAGTCTCTCAAAGAAAGGCATTCTGCAGAAATGGATTCCATCACCATGAGTGGAAGTGAAGCAGATCTGATCAGTGAAATTGAGGACTTGAAAGAAAAGGTGAACGAGCTAGAAAGAGATTGCAATGAGCTGACGGAAGAAAACTTAGAGCTCTTATTCAAGCTTAGGACAGCGAAGCAAAAAACGTCAGGGGGATACACACCTGTTGAACTTCCAGCCAGTGAGCTTTTAGTAGATTCTTTTACCAGCTTTGAGTCCAAAGTGACTGAAAATAAACCCCAATTACAGAATGCAGAGGAGAAGCTTAACAAGAAAGTGCTTGGGGAGATTACAAATAATAATGATTCTTCAGTTCAAGTACTTGACAGCTTGAAAATGGAACTAGAAAGTAAAGTAAATGAGCTGGGTAAAGAATTGACAGAGAAGACGTTCAAGATAGAAAAGCTTGAGGTGAACTTGCTAACCAAAGAAGAAGAAATCAGTCTTCTAAGAGGGGTCCAAAATGAATTACAAGCTGAGGTTTCCAACCTCCAGAAGGAAAAAATAGAGCTAGAGGAACACATGGAAGTTGTATTAAGGGAAAGCGATATCAGCTCTAAGTGTTTGAATGATTTGCGGAATGAATTAATGGTGCTTAGCAGAAGTGTGAATTCCCATGTTTCCACACATAAGGTACTTGAAAGGAAATCTTCGGAGCTAGAAGCTGATAAATGTGAACTGGACCTTCATCTATCAGAGCTTGAACAAGAAAACATACAGTTGTCAGCACGTATTTCTGCACTTGAAGCTCAACAAAGATGCTTGACAGATGAAAAAGAGGCCAATCAGTTAGAATTAGAGAATTCCCAATCTTATTCTCTGAGTCTCCAAGATGAGATTAGTAGATTAAACATTGAGATGGAATCTGATAAAGTTGAACTGAAACAGAAATTAAAGGACTTAGAGAGGCAGTGGTCAGGAGCTCGAGAAGAATGTGAATTTCTGAAAAGGGCAAACCCGAAGCTACAAGCTACTGCTGAGAACCTCATTGAGGAGTGCAATTCTATTCAGAATTCAAATGAAGAGTTGAGGAAGCAAAAGCTGGAGTTGCATCAGCTCTGTTCCCTCTTGGAGGCAAAGTTGAATCAATCGCAGGAGAGCTTTACAAATTGCTCCAAAAGAGTTGAAGTGCTGGAAAAAGATCTTTCTTTAATGCTGGAAAATGTCACCTCAAAAGAGGAGAGCCTGAATTCAGAATTAGATGCACTTCTTGATGAAAACATGAAATATAAGGAGAAACTTACATTGGAAGAAAGTTTGTTCAACAAGATGTATTTGGAGAAGACGATTGAAGTTGAGAGCCTTCAGGAAGAGGTAGAACGACTTACCAAGCAATTTGCTGCTACTCATGAAGAAAGAGAAGGACTAGCTTCAGATGCTGTCCAGGAAGCATCCAGACTTCGAACTGAGAAGGAAATGCTTCAATCTGCTCTCCAAGAGTTTCAATCTAAAGCGATACAGATGGAGGATGAATTGAATATTATGAGAACAGAATCTGAAGTGAAGTTACAAGGCCTATTCGCTGAGCTTGCCGATAAACAAAATCAGGAGTCAACAACAGCTGACCATGAAAAGCTATTGAAGTTGTTAGAGAATTACAAATCAAGTGAAGCAAAACTGAAGACCAATGTGAATGATCTTGAACTAAAGCTTACAATTTCTGATTATGAACATCAGCAGGTGGTGGAAGAATCCACCAATATGAAGGTTCAATTTGAGAAACTAACACGTTGTCAGGAGGAAGTTTTGGCTTTCAAGAATGAGCTGGATGCAACCAAGTTCGAGAAAGAAAAATTGGAAGCGTTATTGGATTCAATATCTGAGGAATGTGAAGATCTGAAGGCAGAGAAGAGTTCATTCCATGAGAAGATCTCAACATTGGAGAAGGCCTTATTTGACTTGGAGGATTGCCAACGTAATAAAGTTCTCTTGGAGGAAAAATTTCTGCAGATGGAAGGTGATCTAACTGCAGAAGAGGCTTTTTGTGCACAGTATGCCGAGATGAAGAATGAGCTCAACCAGATCAGGAGAGCCAACGAGCAGTATCAGCTGGAAATACAGCTACTTGAGGACGAGAGAAGTGAGTTCCTAAGAAAGTCTCAAGCCCTTGAACAAGAATTGAAAGTGACAAAGGAAGAAAAACAGAAGGATTGTAGCAGTCCAATTTCCAGCTCATCAAAAGGCGGCACCAGAGTTATTCCTGTTGGTGAAGATATGAAGGTCCCAAAAAATGAAATGGTGAGGAACAGCATTCACTGTCGTGATTTTAGAAGGAAGCCAATTTTGAAGAATGGTCAGGTGCAAGGAAATGCAACAGATCAGAATTATCCGTATAGAAACCAGCATCAAAGAGAGGATGATAACGGAGTTGAATTTCATGATGAAAGTCCAAATGACGTTGAAGAATTTGGTTTAAAGATTAAGTTGCTTGAAGATGAACTTGTTAAGGCATTGGAAGCAAATAACACTTACAAAGTTCAACTTGATAGGTACTTGTCTGAGGCTCGACACAATCATGCAGATGCTCGTCGAAATTCAAAAGCTGAAGAAAGACACGAACGCTCAAGGTCATCTCTAGAGATGGAACTGAAAGACATTCGCGAGCGCTACTTGCACATGAGCCTGAGATACGCGGAGGTAGAAGCTCAGCGCGAAGAACTTGTCATGAAGCTCAAAGTAGCCAAGGGTGGCAAAAGGTGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1442

Amino Acids

165.09

Weight (kDa)

4.95

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 24 - 140 9.4e-13 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3289
Acc16I TGCGCA 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 3289
AccB1I GGYRCC 1 cut(s) 3806
AccB7I CCANNNNNTGG 2 cut(s) 93, 1258
AccI GTMKAC 1 cut(s) 1316
AccII CGCG 4 cut(s) 1032, 4221, 4253, 4272
AciI CCGC 7 cut(s) 503, 869, 899, 1185, 2197, 3804, 4253
AclWI GGATC 3 cut(s) 285, 972, 1486
AcuI CTGAAG 8 cut(s) 1364, 1914, 2909, 2976, 3138, 3260, 3479, 4185
AfaI GTAC 5 cut(s) 709, 838, 1941, 2256, 4112
AfeI AGCGCT 1 cut(s) 4226
AfiI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 1207, 1258, 2116, 2611, 2692
AflIII ACRYGT 1 cut(s) 3347
AjnI CCWGG 6 cut(s) 78, 547, 770, 926, 1251, 3003
AjuI GAANNNNNNNTTGG 4 cut(s) 2849, 2881, 3398, 3430
AleI CACNNNNGTG 1 cut(s) 3879
AloI GAACNNNNNNTCC 6 cut(s) 41, 73, 766, 798, 1481, 1513
Alw21I GWGCWC 4 cut(s) 1734, 2545, 3610, 3636
Alw26I GTCTC 8 cut(s) 188, 452, 801, 1259, 1508, 1590, 2450, 3723
Alw44I GTGCAC 1 cut(s) 3606
AlwI GGATC 3 cut(s) 285, 972, 1486
AlwNI CAGNNNCTG 3 cut(s) 1258, 2999, 3116
Ama87I CYCGRG 2 cut(s) 1136, 2544
Aor51HI AGCGCT 1 cut(s) 4226
AoxI GGCC 5 cut(s) 23, 72, 2400, 3133, 3507
ApaLI GTGCAC 1 cut(s) 3606
ApeKI GCWGC 4 cut(s) 591, 1187, 1384, 2960
ArsI GACNNNNNNTTYG 4 cut(s) 89, 121, 4266, 4298
AseI ATTAAT 2 cut(s) 777, 2207
Asp700I GAANNNNTTC 4 cut(s) 1151, 2009, 3039, 3561
AspLEI GCGC 3 cut(s) 171, 4227, 4272
AspS9I GGNCC 4 cut(s) 73, 2077, 2302, 3841
AsuC2I CCSGG 2 cut(s) 540, 808
AsuHPI GGTGA 8 cut(s) 1337, 1615, 1693, 2044, 2779, 3590, 3839, 3871
AsuII TTCGAA 2 cut(s) 236, 3022
AvaI CYCGRG 2 cut(s) 1136, 2544
AvaII GGWCC 3 cut(s) 2077, 2302, 3841
BaeGI GKGCMC 1 cut(s) 3610
BanI GGYRCC 1 cut(s) 3806
BanII GRGCYC 4 cut(s) 614, 1734, 2545, 3636
BarI GAAGNNNNNNTAC 2 cut(s) 3111, 3143
BbsI GAAGAC 6 cut(s) 225, 685, 2012, 2057, 2909, 3248
Bbv12I GWGCWC 4 cut(s) 1734, 2545, 3610, 3636
BbvCI CCTCAGC 1 cut(s) 2097
BbvI GCAGC 4 cut(s) 603, 1199, 1371, 2947
BccI CCATC 8 cut(s) 238, 1028, 1118, 1628, 2473, 3076, 3565, 4195
BciT130I CCWGG 6 cut(s) 80, 549, 772, 928, 1253, 3005
BciVI GTATCC 1 cut(s) 1769
BclI TGATCA 2 cut(s) 1341, 1650
BcnI CCSGG 2 cut(s) 540, 808
BcoDI GTCTC 8 cut(s) 188, 452, 801, 1259, 1508, 1590, 2450, 3723
BfaI CTAG 6 cut(s) 1691, 1964, 2132, 2279, 2987, 4196
BfmI CTRYAG 6 cut(s) 123, 1202, 1310, 1605, 3566, 3588
BfoI RGCGCY 1 cut(s) 4228
BfuAI ACCTGC 1 cut(s) 3289
BfuI GTATCC 1 cut(s) 1769
BglII AGATCT 4 cut(s) 1645, 2761, 3454, 3489
BisI GCNGC 6 cut(s) 592, 870, 1188, 1385, 2961, 3805
BlpI GCTNAGC 3 cut(s) 2216, 3144, 4266
BlsI GCNGC 6 cut(s) 593, 871, 1189, 1386, 2962, 3806
BmcAI AGTACT 1 cut(s) 1941
Bme1390I CCNGG 8 cut(s) 80, 540, 549, 772, 808, 928, 1253, 3005
Bme18I GGWCC 3 cut(s) 2077, 2302, 3841
BmeT110I CYCGRG 2 cut(s) 1136, 2544
BmgT120I GGNCC 4 cut(s) 73, 2077, 2302, 3841
BmiI GGNNCC 5 cut(s) 84, 613, 2078, 3808, 3843
BmrFI CCNGG 8 cut(s) 80, 540, 549, 772, 808, 928, 1253, 3005
BmrI ACTGGG 1 cut(s) 141
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 6 cut(s) 225, 685, 2012, 2057, 2909, 3248
BplI GAGNNNNNCTC 4 cut(s) 1119, 1151, 3476, 3508
BpmI CTGGAG 5 cut(s) 62, 339, 1235, 2096, 2684
Bpu10I CCTNAGC 2 cut(s) 1745, 2097
Bpu1102I GCTNAGC 3 cut(s) 2216, 3144, 4266
Bpu14I TTCGAA 2 cut(s) 236, 3022
BpuEI CTTGAG 5 cut(s) 1094, 2048, 3705, 3707, 4168
BpuMI CCSGG 2 cut(s) 540, 808
Bsa29I ATCGAT 1 cut(s) 976
BsaAI YACGTR 1 cut(s) 2351
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 5 cut(s) 276, 301, 548, 1446, 4302
BsaXI ACNNNNNCTCC 4 cut(s) 1481, 1511, 2684, 2714
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 93, 307, 1207, 1258, 2116, 2611, 2692
Bse1I ACTGG 5 cut(s) 136, 356, 1101, 1800, 2304
Bse3DI GCAATG 2 cut(s) 1089, 1711
BseBI CCWGG 6 cut(s) 80, 549, 772, 928, 1253, 3005
BseCI ATCGAT 1 cut(s) 976
BseDI CCNNGG 5 cut(s) 276, 301, 548, 1446, 4302
BseGI GGATG 7 cut(s) 297, 871, 1129, 3011, 3094, 3394, 3982
BseLI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 1207, 1258, 2116, 2611, 2692
BseMI GCAATG 2 cut(s) 1089, 1711
BseNI ACTGG 5 cut(s) 136, 356, 1101, 1800, 2304
BseRI GAGGAG 3 cut(s) 1892, 2627, 2813
BseSI GKGCMC 1 cut(s) 3610
BseXI GCAGC 4 cut(s) 603, 1199, 1371, 2947
BseYI CCCAGC 1 cut(s) 1984
BsgI GTGCAG 2 cut(s) 400, 2343
Bsh1236I CGCG 4 cut(s) 1032, 4221, 4253, 4272
BshFI GGCC 5 cut(s) 25, 74, 2402, 3135, 3509
BshNI GGYRCC 1 cut(s) 3806
BshVI ATCGAT 1 cut(s) 976
BsiHKAI GWGCWC 4 cut(s) 1734, 2545, 3610, 3636
BsiHKCI CYCGRG 2 cut(s) 1136, 2544
BsiSI CCGG 3 cut(s) 294, 540, 808
BslFI GGGAC 3 cut(s) 110, 1183, 3827
BslI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 1207, 1258, 2116, 2611, 2692
BsmAI GTCTC 8 cut(s) 188, 452, 801, 1259, 1508, 1590, 2450, 3723
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 3 cut(s) 110, 1183, 3827
BsmI GAATGC 6 cut(s) 928, 1084, 1151, 1600, 1876, 3870
BsnI GGCC 5 cut(s) 25, 74, 2402, 3135, 3509
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 2 cut(s) 1136, 2544
Bsp119I TTCGAA 2 cut(s) 236, 3022
Bsp1286I GDGCHC 5 cut(s) 614, 1734, 2545, 3610, 3636
Bsp1720I GCTNAGC 3 cut(s) 2216, 3144, 4266
Bsp19I CCATGG 1 cut(s) 276
Bsp68I TCGCGA 1 cut(s) 4221
BspACI CCGC 7 cut(s) 503, 869, 899, 1185, 2197, 3804, 4253
BspANI GGCC 5 cut(s) 25, 74, 2402, 3135, 3509
BspDI ATCGAT 1 cut(s) 976
BspFNI CGCG 4 cut(s) 1032, 4221, 4253, 4272
BspHI TCATGA 5 cut(s) 496, 1462, 2968, 3997, 4284
BspLI GGNNCC 5 cut(s) 84, 613, 2078, 3808, 3843
BspMAI CTGCAG 4 cut(s) 1206, 1609, 3570, 3592
BspMI ACCTGC 1 cut(s) 3289
BspPI GGATC 3 cut(s) 285, 972, 1486
BspT104I TTCGAA 2 cut(s) 236, 3022
BspT107I GGYRCC 1 cut(s) 3806
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 1089, 1711
BsrI ACTGG 5 cut(s) 136, 356, 1101, 1800, 2304
BssECI CCNNGG 5 cut(s) 276, 301, 548, 1446, 4302
BssT1I CCWWGG 3 cut(s) 276, 301, 4302
Bst2UI CCWGG 6 cut(s) 80, 549, 772, 928, 1253, 3005
Bst4CI ACNGT 6 cut(s) 182, 1314, 1447, 2340, 3612, 3878
BstAPI GCANNNNNTGC 3 cut(s) 1069, 1393, 3614
BstBAI YACGTR 1 cut(s) 2351
BstBI TTCGAA 2 cut(s) 236, 3022
BstC8I GCNNGC 4 cut(s) 27, 990, 3150, 4223
BstDSI CCRYGG 2 cut(s) 276, 1446
BstENI CCTNNNNNAGG 4 cut(s) 305, 2114, 2609, 2690
BstF5I GGATG 7 cut(s) 297, 871, 1129, 3011, 3094, 3394, 3982
BstFNI CGCG 4 cut(s) 1032, 4221, 4253, 4272
BstH2I RGCGCY 1 cut(s) 4228
BstHHI GCGC 3 cut(s) 171, 4227, 4272
BstMAI GTCTC 8 cut(s) 188, 452, 801, 1259, 1508, 1590, 2450, 3723
BstNI CCWGG 6 cut(s) 80, 549, 772, 928, 1253, 3005
BstNSI RCATGY 1 cut(s) 899
BstSCI CCNGG 8 cut(s) 78, 538, 547, 770, 806, 926, 1251, 3003
BstSFI CTRYAG 6 cut(s) 123, 1202, 1310, 1605, 3566, 3588
BstSLI GKGCMC 1 cut(s) 3610
BstUI CGCG 4 cut(s) 1032, 4221, 4253, 4272
BstV1I GCAGC 4 cut(s) 603, 1199, 1371, 2947
BstV2I GAAGAC 6 cut(s) 225, 685, 2012, 2057, 2909, 3248
BstX2I RGATCY 5 cut(s) 290, 1645, 2761, 3454, 3489
BstYI RGATCY 5 cut(s) 290, 1645, 2761, 3454, 3489
Bsu15I ATCGAT 1 cut(s) 976
BsuI GTATCC 1 cut(s) 1769
BsuRI GGCC 5 cut(s) 25, 74, 2402, 3135, 3509
BsuTUI ATCGAT 1 cut(s) 976
BtgI CCRYGG 2 cut(s) 276, 1446
BtgZI GCGATG 2 cut(s) 572, 1047
BtsCI GGATG 7 cut(s) 297, 871, 1129, 3011, 3094, 3394, 3982
BtsI GCAGTG 2 cut(s) 168, 2537
BtsIMutI CAGTG 9 cut(s) 168, 191, 445, 840, 1108, 1660, 1807, 2537, 3874
BtuMI TCGCGA 1 cut(s) 4221
BveI ACCTGC 1 cut(s) 3289
Cac8I GCNNGC 4 cut(s) 27, 990, 3150, 4223
CaiI CAGNNNCTG 3 cut(s) 1258, 2999, 3116
CciI TCATGA 5 cut(s) 496, 1462, 2968, 3997, 4284
CfoI GCGC 3 cut(s) 171, 4227, 4272
Cfr13I GGNCC 4 cut(s) 73, 2077, 2302, 3841
ClaI ATCGAT 1 cut(s) 976
Csp6I GTAC 5 cut(s) 708, 837, 1940, 2255, 4111
CviQI GTAC 5 cut(s) 708, 837, 1940, 2255, 4111
DraI TTTAAA 2 cut(s) 1374, 4035
Ecl136II GAGCTC 3 cut(s) 1732, 2543, 3634
Eco130I CCWWGG 3 cut(s) 276, 301, 4302
Eco147I AGGCCT 3 cut(s) 25, 3135, 3509
Eco24I GRGCYC 4 cut(s) 614, 1734, 2545, 3636
Eco31I GGTCTC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 2170
Eco47I GGWCC 3 cut(s) 2077, 2302, 3841
Eco47III AGCGCT 1 cut(s) 4226
Eco53kI GAGCTC 3 cut(s) 1732, 2543, 3634
Eco57I CTGAAG 8 cut(s) 1364, 1914, 2909, 2976, 3138, 3260, 3479, 4185
Eco88I CYCGRG 2 cut(s) 1136, 2544
EcoICRI GAGCTC 3 cut(s) 1732, 2543, 3634
EcoNI CCTNNNNNAGG 4 cut(s) 305, 2114, 2609, 2690
EcoO109I RGGNCCY 2 cut(s) 73, 3841
EcoRI GAATTC 6 cut(s) 789, 802, 2230, 2422, 2632, 2809
EcoRII CCWGG 6 cut(s) 78, 547, 770, 926, 1251, 3003
EcoRV GATATC 1 cut(s) 2170
EcoT14I CCWWGG 3 cut(s) 276, 301, 4302
EcoT38I GRGCYC 4 cut(s) 614, 1734, 2545, 3636
ErhI CCWWGG 3 cut(s) 276, 301, 4302
Esp3I CGTCTC 1 cut(s) 452
FalI AAGNNNNNCTT 6 cut(s) 2254, 2286, 4044, 4076, 4086, 4118
FaqI GGGAC 3 cut(s) 110, 1183, 3827
FauNDI CATATG 1 cut(s) 1021
FbaI TGATCA 2 cut(s) 1341, 1650
FblI GTMKAC 1 cut(s) 1316
Fnu4HI GCNGC 6 cut(s) 592, 870, 1188, 1385, 2961, 3805
FokI GGATG 7 cut(s) 284, 878, 1136, 2998, 3101, 3401, 3989
FriOI GRGCYC 4 cut(s) 614, 1734, 2545, 3636
Fsp4HI GCNGC 6 cut(s) 592, 870, 1188, 1385, 2961, 3805
FspBI CTAG 6 cut(s) 1691, 1964, 2132, 2279, 2987, 4196
FspI TGCGCA 1 cut(s) 170
GlaI GCGC 3 cut(s) 170, 4226, 4271
GluI GCNGC 6 cut(s) 592, 870, 1188, 1385, 2961, 3805
GsaI CCCAGC 1 cut(s) 1988
GsuI CTGGAG 5 cut(s) 62, 339, 1235, 2096, 2684
HaeII RGCGCY 1 cut(s) 4228
HaeIII GGCC 5 cut(s) 25, 74, 2402, 3135, 3509
HapII CCGG 3 cut(s) 294, 540, 808
HhaI GCGC 3 cut(s) 171, 4227, 4272
Hin6I GCGC 3 cut(s) 169, 4225, 4270
HinP1I GCGC 3 cut(s) 169, 4225, 4270
HincII GTYRAC 2 cut(s) 1317, 3174
HindII GTYRAC 2 cut(s) 1317, 3174
HindIII AAGCTT 6 cut(s) 263, 1376, 1742, 1883, 2024, 3269
HpaII CCGG 3 cut(s) 294, 540, 808
HphI GGTGA 8 cut(s) 1337, 1615, 1693, 2044, 2779, 3590, 3839, 3871
Hpy166II GTNNAC 9 cut(s) 189, 584, 1317, 1532, 1684, 2035, 2297, 3174, 3608
Hpy8I GTNNAC 9 cut(s) 189, 584, 1317, 1532, 1684, 2035, 2297, 3174, 3608
Hpy99I CGWCG 1 cut(s) 4152
HpyCH4III ACNGT 6 cut(s) 182, 1314, 1447, 2340, 3612, 3878
HpyCH4IV ACGT 6 cut(s) 1766, 2009, 2350, 3349, 3535, 4017
HpySE526I ACGT 6 cut(s) 1766, 2009, 2350, 3349, 3535, 4017
HspAI GCGC 3 cut(s) 169, 4225, 4270
Ksp22I TGATCA 2 cut(s) 1341, 1650
LmnI GCTCC 7 cut(s) 358, 617, 1006, 1255, 2275, 2540, 2738
Lsp1109I GCAGC 4 cut(s) 603, 1199, 1371, 2947
MaeI CTAG 6 cut(s) 1691, 1964, 2132, 2279, 2987, 4196
MaeII ACGT 6 cut(s) 1766, 2009, 2350, 3349, 3535, 4017
MaeIII GTNAC 6 cut(s) 877, 891, 1843, 2785, 3126, 3745
MfeI CAATTG 4 cut(s) 114, 141, 1269, 1391
MflI RGATCY 5 cut(s) 290, 1645, 2761, 3454, 3489
MhlI GDGCHC 5 cut(s) 614, 1734, 2545, 3610, 3636
MlyI GAGTC 9 cut(s) 239, 458, 562, 593, 1492, 1592, 1844, 2452, 3179
MmeI TCCRAC 2 cut(s) 1188, 2130
MroXI GAANNNNTTC 4 cut(s) 1151, 2009, 3039, 3561
MslI CAYNNNNRTG 6 cut(s) 1625, 2477, 3320, 3879, 4134, 4140
MspA1I CMGCKG 3 cut(s) 1384, 3182, 3670
MspI CCGG 3 cut(s) 294, 540, 808
MspR9I CCNGG 8 cut(s) 80, 540, 549, 772, 808, 928, 1253, 3005
MunI CAATTG 4 cut(s) 114, 141, 1269, 1391
Mva1269I GAATGC 6 cut(s) 928, 1084, 1151, 1600, 1876, 3870
MvaI CCWGG 6 cut(s) 80, 549, 772, 928, 1253, 3005
MvnI CGCG 4 cut(s) 1032, 4221, 4253, 4272
NciI CCSGG 2 cut(s) 540, 808
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 1021
NlaIV GGNNCC 5 cut(s) 84, 613, 2078, 3808, 3843
NmeAIII GCCGAG 2 cut(s) 581, 3643
NmuCI GTSAC 3 cut(s) 1843, 2785, 3745
NruI TCGCGA 1 cut(s) 4221
NsbI TGCGCA 1 cut(s) 170
NspI RCATGY 1 cut(s) 899
NspV TTCGAA 2 cut(s) 236, 3022
OliI CACNNNNGTG 1 cut(s) 3879
PaeR7I CTCGAG 2 cut(s) 1136, 2544
PagI TCATGA 5 cut(s) 496, 1462, 2968, 3997, 4284
PaqCI CACCTGC 1 cut(s) 3289
PceI AGGCCT 3 cut(s) 25, 3135, 3509
PctI GAATGC 6 cut(s) 928, 1084, 1151, 1600, 1876, 3870
PdmI GAANNNNTTC 4 cut(s) 1151, 2009, 3039, 3561
PflMI CCANNNNNTGG 2 cut(s) 93, 1258
PfoI TCCNGGA 5 cut(s) 538, 770, 806, 1251, 3003
PkrI GCNGC 6 cut(s) 593, 871, 1189, 1386, 2962, 3806
PleI GAGTC 9 cut(s) 238, 457, 561, 593, 1491, 1591, 1843, 2451, 3178
PpsI GAGTC 9 cut(s) 238, 457, 561, 593, 1491, 1591, 1843, 2451, 3178
Ppu21I YACGTR 1 cut(s) 2351
PpuMI RGGWCCY 1 cut(s) 3841
PshBI ATTAAT 2 cut(s) 777, 2207
Psp124BI GAGCTC 3 cut(s) 1734, 2545, 3636
Psp5II RGGWCCY 1 cut(s) 3841
Psp6I CCWGG 6 cut(s) 78, 547, 770, 926, 1251, 3003
PspFI CCCAGC 1 cut(s) 1984
PspGI CCWGG 6 cut(s) 78, 547, 770, 926, 1251, 3003
PspN4I GGNNCC 5 cut(s) 84, 613, 2078, 3808, 3843
PspPI GGNCC 4 cut(s) 73, 2077, 2302, 3841
PspPPI RGGWCCY 1 cut(s) 3841
PstI CTGCAG 4 cut(s) 1206, 1609, 3570, 3592
PstNI CAGNNNCTG 3 cut(s) 1258, 2999, 3116
PsuI RGATCY 5 cut(s) 290, 1645, 2761, 3454, 3489
PvuII CAGCTG 3 cut(s) 1384, 3182, 3670
RruI TCGCGA 1 cut(s) 4221
RsaI GTAC 5 cut(s) 709, 838, 1941, 2256, 4112
RsaNI GTAC 5 cut(s) 708, 837, 1940, 2255, 4111
RseI CAYNNNNRTG 6 cut(s) 1625, 2477, 3320, 3879, 4134, 4140
SacI GAGCTC 3 cut(s) 1734, 2545, 3636
SalI GTCGAC 1 cut(s) 1315
SatI GCNGC 6 cut(s) 592, 870, 1188, 1385, 2961, 3805
Sau96I GGNCC 4 cut(s) 73, 2077, 2302, 3841
ScaI AGTACT 1 cut(s) 1941
SchI GAGTC 9 cut(s) 239, 458, 562, 593, 1492, 1592, 1844, 2452, 3179
ScrFI CCNGG 8 cut(s) 80, 540, 549, 772, 808, 928, 1253, 3005
SduI GDGCHC 5 cut(s) 614, 1734, 2545, 3610, 3636
SfcI CTRYAG 6 cut(s) 123, 1202, 1310, 1605, 3566, 3588
Sfr274I CTCGAG 2 cut(s) 1136, 2544
SfuI TTCGAA 2 cut(s) 236, 3022
SinI GGWCC 3 cut(s) 2077, 2302, 3841
SlaI CTCGAG 2 cut(s) 1136, 2544
SmiMI CAYNNNNRTG 6 cut(s) 1625, 2477, 3320, 3879, 4134, 4140
SmlI CTYRAG 7 cut(s) 1073, 1136, 2027, 2544, 3686, 3720, 4183
SmoI CTYRAG 7 cut(s) 1073, 1136, 2027, 2544, 3686, 3720, 4183
SseBI AGGCCT 3 cut(s) 25, 3135, 3509
SsiI CCGC 7 cut(s) 503, 869, 899, 1185, 2197, 3804, 4253
SspI AATATT 2 cut(s) 1231, 3100
SspMI CTAG 6 cut(s) 1691, 1964, 2132, 2279, 2987, 4196
SstI GAGCTC 3 cut(s) 1734, 2545, 3636
StuI AGGCCT 3 cut(s) 25, 3135, 3509
StyD4I CCNGG 8 cut(s) 78, 538, 547, 770, 806, 926, 1251, 3003
StyI CCWWGG 3 cut(s) 276, 301, 4302
TaaI ACNGT 6 cut(s) 182, 1314, 1447, 2340, 3612, 3878
TaiI ACGT 6 cut(s) 1769, 2012, 2353, 3352, 3538, 4020
TatI WGTACW 2 cut(s) 707, 1939
TauI GCSGC 2 cut(s) 872, 3807
TscAI CASTG 9 cut(s) 168, 191, 445, 847, 1108, 1660, 1807, 2537, 3881
TseFI GTSAC 3 cut(s) 1843, 2785, 3745
TseI GCWGC 4 cut(s) 591, 1187, 1384, 2960
Tsp45I GTSAC 3 cut(s) 1843, 2785, 3745
TspGWI ACGGA 5 cut(s) 189, 735, 1730, 3939, 3999
TspRI CASTG 9 cut(s) 168, 191, 445, 847, 1108, 1660, 1807, 2537, 3881
Van91I CCANNNNNTGG 2 cut(s) 93, 1258
VneI GTGCAC 1 cut(s) 3606
VpaK11BI GGWCC 3 cut(s) 2077, 2302, 3841
VspI ATTAAT 2 cut(s) 777, 2207
XagI CCTNNNNNAGG 4 cut(s) 305, 2114, 2609, 2690
XbaI TCTAGA 1 cut(s) 4195
XceI RCATGY 1 cut(s) 899
XhoI CTCGAG 2 cut(s) 1136, 2544
XmiI GTMKAC 1 cut(s) 1316
XmnI GAANNNNTTC 4 cut(s) 1151, 2009, 3039, 3561
XspI CTAG 6 cut(s) 1691, 1964, 2132, 2279, 2987, 4196
ZrmI AGTACT 1 cut(s) 1941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.