pycom14g11890

Transmembrane protein 120

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006071
Physical Location & Seq
Forward (+)
14958200 .. 14962217
4018 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
ATGGGGGATGCCAAGAGGAACACTTCTTCTTCTTCTTCCGGCAGTGACGAGGAGATACGAGCACAAGTGGCCAAGGCGGTGGAGGAGGTGAAGGAGCTGCAGGACAAAGCTGCTTCCTTTGTATCCAGAGCCGCCACCGACGAGCAATCCCTCCGCCAGCGCGCCCAATCTCTCGATTCCTCCATCCGCCGCCTCCGCTCCCAGCTTTATTCACTGCTTTCCAGCAAGGCCTTGGATCACAAGTTAGCAGAAAAGCTGGAAGAAGATTTGCAGAGGGCCACATGTGTGTTGGTGGATGGCGAGGCCGCTTCGTTTCTTCCTGGGAAATCTCAGGGAGGAGGGTTTCTAAGGATGTTTCTCGGTCCTATCAATGTGCGTTCCTCTAGCAAAGATATCCAACTCAAGGTTAAAGAGGAATACAACGCTTACAGAGATAGAACTGCCCTGCTATTCCTTATTTTTCCAGCAACACTACTTATACTAAGATCTTGGATTTGGGATGGATGTTTGCCAGCGTTTCCAGTTCAGGCTTACCAGGCATGGCTGTTATTCCTCTACACTGGTTTGGCCCTGCGAGAAAACATACTGAGAGTAAATGGAAGTGATATTCGTCCATGGTGGATATACCATCACTATTGTGCTGTATTTATGGCCCTTGTTAGTCTCACATGGGAGATTAAAGGACAACCAAATTGTTCCCAAAAGCAGAGAGGTGTGCAACTTTTCCTACAATGGGCTATGATGCAAGGAGTTGCTATGCTTTTACAAAATAGATATCAACGCCAGAGACTCTATACTCGAATTGCATTGGGAAAGGCCAAAAGAATGGATGTGGTCTGGGGAGAAACAGCAGGCGTAGATGGTCAATTGTGGATTCTATGTCCAATACTATTTGTTTTACAGGGTTTTGAGGCATATGTCGGAATACAGTTGCTCACAACTGCTTTTATTGGCGTTGTTTCGGAATGGCAGGTGAGTTTTTGCGGCGTGCTGTTAGTTTTAATGGCAGTTGGGAACTTTGTAAATACGGTACAGACGCTTATGGTGAAGTCGAGGTTTAAGGCAAAGATGAAAAGAAGTAAGAGCAAGCAGGATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMPIT

Protein Analysis

367

Amino Acids

41.39

Weight (kDa)

9.56

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM120A-B PF07851 22 - 353 6.4e-99 TMEM120A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 961
Acc36I ACCTGC 1 cut(s) 961
AccBSI CCGCTC 1 cut(s) 198
AccII CGCG 1 cut(s) 162
AciI CCGC 8 cut(s) 77, 132, 154, 187, 190, 196, 306, 984
AclWI GGATC 1 cut(s) 243
AcoI YGGCCR 1 cut(s) 69
AfaI GTAC 1 cut(s) 1032
AfiI CCNNNNNNNGG 2 cut(s) 403, 733
AflIII ACRYGT 1 cut(s) 281
AjnI CCWGG 2 cut(s) 319, 534
AleI CACNNNNGTG 2 cut(s) 284, 636
AluBI AGCT 4 cut(s) 97, 110, 205, 256
AluI AGCT 4 cut(s) 97, 110, 205, 256
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 2 cut(s) 668, 781
AlwI GGATC 1 cut(s) 243
AlwNI CAGNNNCTG 1 cut(s) 1096
AoxI GGCC 7 cut(s) 69, 228, 276, 303, 567, 651, 816
ApeKI GCWGC 2 cut(s) 97, 110
AspLEI GCGC 2 cut(s) 162, 164
AspS9I GGNCC 4 cut(s) 276, 362, 568, 652
AsuHPI GGTGA 3 cut(s) 100, 985, 1057
AvaII GGWCC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 2 cut(s) 84, 97
BccI CCATC 5 cut(s) 191, 290, 494, 636, 854
BciT130I CCWGG 2 cut(s) 321, 536
BciVI GTATCC 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 668, 781
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 98, 1097
BfuAI ACCTGC 1 cut(s) 961
BfuI GTATCC 1 cut(s) 133
BglII AGATCT 1 cut(s) 485
BisI GCNGC 6 cut(s) 98, 111, 132, 190, 306, 985
BlsI GCNGC 6 cut(s) 99, 112, 133, 191, 307, 986
Bme1390I CCNGG 2 cut(s) 321, 536
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 4 cut(s) 276, 362, 568, 652
BmrFI CCNGG 2 cut(s) 321, 536
BmsI GCATC 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 386
BsaJI CCNNGG 4 cut(s) 72, 231, 320, 614
Bsc4I CCNNNNNNNGG 2 cut(s) 403, 733
Bse1I ACTGG 2 cut(s) 521, 565
BseBI CCWGG 2 cut(s) 321, 536
BseDI CCNNGG 4 cut(s) 72, 231, 320, 614
BseGI GGATG 7 cut(s) 13, 183, 301, 357, 505, 509, 835
BseLI CCNNNNNNNGG 2 cut(s) 403, 733
BseMII CTCAG 2 cut(s) 344, 578
BseNI ACTGG 2 cut(s) 521, 565
BsePI GCGCGC 1 cut(s) 160
BseRI GAGGAG 3 cut(s) 65, 98, 351
BseXI GCAGC 2 cut(s) 84, 97
BseYI CCCAGC 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 7 cut(s) 71, 230, 278, 305, 569, 653, 818
BsiHKAI GWGCWC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 2 cut(s) 403, 733
BsmAI GTCTC 2 cut(s) 668, 781
BsnI GGCC 7 cut(s) 71, 230, 278, 305, 569, 653, 818
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 3 cut(s) 235, 485, 1093
Bsp19I CCATGG 1 cut(s) 614
BspACI CCGC 8 cut(s) 77, 132, 154, 187, 190, 196, 306, 984
BspANI GGCC 7 cut(s) 71, 230, 278, 305, 569, 653, 818
BspCNI CTCAG 2 cut(s) 343, 579
BspFNI CGCG 1 cut(s) 162
BspMAI CTGCAG 1 cut(s) 102
BspMI ACCTGC 1 cut(s) 961
BspPI GGATC 1 cut(s) 243
BsrBI CCGCTC 1 cut(s) 198
BsrI ACTGG 2 cut(s) 521, 565
BssECI CCNNGG 4 cut(s) 72, 231, 320, 614
BssHII GCGCGC 1 cut(s) 160
BssMI GATC 3 cut(s) 235, 485, 1093
BssT1I CCWWGG 3 cut(s) 72, 231, 614
Bst2UI CCWGG 2 cut(s) 321, 536
Bst4CI ACNGT 2 cut(s) 930, 1030
BstC8I GCNNGC 6 cut(s) 158, 162, 513, 853, 989, 1088
BstDEI CTNAG 4 cut(s) 330, 347, 482, 587
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 7 cut(s) 13, 183, 301, 357, 505, 509, 835
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 2 cut(s) 162, 164
BstKTI GATC 3 cut(s) 238, 488, 1096
BstMAI GTCTC 2 cut(s) 668, 781
BstMBI GATC 3 cut(s) 235, 485, 1093
BstMWI GCNNNNNNNGC 3 cut(s) 68, 195, 536
BstNI CCWGG 2 cut(s) 321, 536
BstNSI RCATGY 1 cut(s) 285
BstSCI CCNGG 2 cut(s) 319, 534
BstSFI CTRYAG 2 cut(s) 98, 1097
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 2 cut(s) 84, 97
BstX2I RGATCY 2 cut(s) 485, 1093
BstXI CCANNNNNNTGG 2 cut(s) 79, 826
BstYI RGATCY 2 cut(s) 485, 1093
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 7 cut(s) 71, 230, 278, 305, 569, 653, 818
BtgI CCRYGG 1 cut(s) 614
BtsCI GGATG 7 cut(s) 13, 183, 301, 357, 505, 509, 835
BtsI GCAGTG 2 cut(s) 49, 212
BtsIMutI CAGTG 3 cut(s) 49, 212, 558
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 6 cut(s) 158, 162, 513, 853, 989, 1088
CaiI CAGNNNCTG 1 cut(s) 1096
CfoI GCGC 2 cut(s) 162, 164
Cfr13I GGNCC 4 cut(s) 276, 362, 568, 652
CseI GACGC 1 cut(s) 1045
Csp6I GTAC 1 cut(s) 1031
CviAII CATG 4 cut(s) 282, 540, 615, 669
CviQI GTAC 1 cut(s) 1031
DdeI CTNAG 4 cut(s) 330, 347, 482, 587
DpnI GATC 3 cut(s) 237, 487, 1095
DpnII GATC 3 cut(s) 235, 485, 1093
EaeI YGGCCR 1 cut(s) 69
EciI GGCGGA 2 cut(s) 143, 176
Eco130I CCWWGG 3 cut(s) 72, 231, 614
Eco147I AGGCCT 1 cut(s) 230
Eco32I GATATC 2 cut(s) 394, 776
Eco47I GGWCC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 319, 534
EcoRV GATATC 2 cut(s) 394, 776
EcoT14I CCWWGG 3 cut(s) 72, 231, 614
ErhI CCWWGG 3 cut(s) 72, 231, 614
FaeI CATG 4 cut(s) 285, 543, 618, 672
FatI CATG 4 cut(s) 281, 539, 614, 668
FauNDI CATATG 1 cut(s) 916
Fnu4HI GCNGC 6 cut(s) 98, 111, 132, 190, 306, 985
FokI GGATG 7 cut(s) 20, 170, 308, 364, 512, 516, 842
Fsp4HI GCNGC 6 cut(s) 98, 111, 132, 190, 306, 985
FspBI CTAG 1 cut(s) 384
GlaI GCGC 2 cut(s) 161, 163
GluI GCNGC 6 cut(s) 98, 111, 132, 190, 306, 985
GsaI CCCAGC 1 cut(s) 205
HaeIII GGCC 7 cut(s) 71, 230, 278, 305, 569, 653, 818
HapII CCGG 1 cut(s) 39
HgaI GACGC 1 cut(s) 1045
HhaI GCGC 2 cut(s) 162, 164
Hin1II CATG 4 cut(s) 285, 543, 618, 672
Hin6I GCGC 2 cut(s) 160, 162
HinP1I GCGC 2 cut(s) 160, 162
HinfI GANTC 3 cut(s) 176, 789, 874
HpaII CCGG 1 cut(s) 39
HphI GGTGA 3 cut(s) 100, 985, 1057
Hpy188I TCNGA 2 cut(s) 923, 964
Hpy188III TCNNGA 2 cut(s) 126, 173
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 2 cut(s) 930, 1030
HpyCH4V TGCA 5 cut(s) 100, 271, 718, 745, 806
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 195, 536
HpyF3I CTNAG 4 cut(s) 330, 347, 482, 587
Hsp92II CATG 4 cut(s) 285, 543, 618, 672
HspAI GCGC 2 cut(s) 160, 162
Kzo9I GATC 3 cut(s) 235, 485, 1093
LmnI GCTCC 2 cut(s) 94, 203
Lsp1109I GCAGC 2 cut(s) 84, 97
LweI GCATC 1 cut(s) 732
MaeI CTAG 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 44
MalI GATC 3 cut(s) 237, 487, 1095
MbiI CCGCTC 1 cut(s) 198
MboI GATC 3 cut(s) 235, 485, 1093
MboII GAAGA 7 cut(s) 18, 21, 24, 27, 272, 275, 308
MfeI CAATTG 1 cut(s) 866
MflI RGATCY 2 cut(s) 485, 1093
MhlI GDGCHC 1 cut(s) 64
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 3 cut(s) 691, 801, 866
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 783
MmeI TCCRAC 2 cut(s) 421, 901
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MseI TTAA 4 cut(s) 408, 678, 1001, 1059
MslI CAYNNNNRTG 2 cut(s) 284, 636
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 2 cut(s) 321, 536
MunI CAATTG 1 cut(s) 866
MvaI CCWGG 2 cut(s) 321, 536
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 68, 195, 536
NcoI CCATGG 1 cut(s) 614
NdeI CATATG 1 cut(s) 916
NdeII GATC 3 cut(s) 235, 485, 1093
NlaIII CATG 4 cut(s) 285, 543, 618, 672
NmuCI GTSAC 1 cut(s) 44
NspI RCATGY 1 cut(s) 285
OliI CACNNNNGTG 2 cut(s) 284, 636
PaqCI CACCTGC 1 cut(s) 961
PauI GCGCGC 1 cut(s) 160
PceI AGGCCT 1 cut(s) 230
PciI ACATGT 1 cut(s) 281
PfeI GAWTC 2 cut(s) 176, 874
PkrI GCNGC 6 cut(s) 99, 112, 133, 191, 307, 986
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
PscI ACATGT 1 cut(s) 281
Psp6I CCWGG 2 cut(s) 319, 534
PspFI CCCAGC 1 cut(s) 201
PspGI CCWGG 2 cut(s) 319, 534
PspPI GGNCC 4 cut(s) 276, 362, 568, 652
PstI CTGCAG 1 cut(s) 102
PstNI CAGNNNCTG 1 cut(s) 1096
PsuI RGATCY 2 cut(s) 485, 1093
PteI GCGCGC 1 cut(s) 160
RsaI GTAC 1 cut(s) 1032
RsaNI GTAC 1 cut(s) 1031
RseI CAYNNNNRTG 2 cut(s) 284, 636
SaqAI TTAA 4 cut(s) 408, 678, 1001, 1059
SatI GCNGC 6 cut(s) 98, 111, 132, 190, 306, 985
Sau3AI GATC 3 cut(s) 235, 485, 1093
Sau96I GGNCC 4 cut(s) 276, 362, 568, 652
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 2 cut(s) 321, 536
SduI GDGCHC 1 cut(s) 64
SetI ASST 9 cut(s) 90, 99, 112, 207, 258, 408, 715, 975, 1058
SfaNI GCATC 1 cut(s) 732
SfcI CTRYAG 2 cut(s) 98, 1097
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 2 cut(s) 284, 636
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 3 cut(s) 691, 801, 866
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 8 cut(s) 77, 132, 154, 187, 190, 196, 306, 984
SspMI CTAG 1 cut(s) 384
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 319, 534
StyI CCWWGG 3 cut(s) 72, 231, 614
TaaI ACNGT 2 cut(s) 930, 1030
TaqI TCGA 3 cut(s) 174, 799, 1052
TaqII GACCGA 1 cut(s) 350
TasI AATT 3 cut(s) 691, 801, 866
TauI GCSGC 4 cut(s) 134, 192, 308, 987
TfiI GAWTC 2 cut(s) 176, 874
Tru1I TTAA 4 cut(s) 408, 678, 1001, 1059
Tru9I TTAA 4 cut(s) 408, 678, 1001, 1059
TscAI CASTG 3 cut(s) 49, 219, 565
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 2 cut(s) 97, 110
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 1 cut(s) 1085
TspRI CASTG 3 cut(s) 49, 219, 565
VpaK11BI GGWCC 1 cut(s) 362
XceI RCATGY 1 cut(s) 285
XcmI CCANNNNNNNNNTGG 2 cut(s) 229, 286
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.