pycom14g17590

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010088
Physical Location & Seq
Forward (+)
19455724 .. 19459433
3710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1470 bp
ATGTATGATTTTATGACGGTGTATGCGTGTTATCTTGCCATCATAGGTGGCCTTGGAGTTACTCTGATACTGTTAGCTGTGTATCAGAAAGCTTTGCCAGCTCTTCCCGTGTCAATAATGCTCGGCGTACTGTTTTATGTATTGACACGGCTCTTACTGGAAGTTTTCGTTGTACAATTCCAACACCTGCAAAGTTGGGAGAAGGGAGGATGCTGTATGGATCGGAGCCTCGGAGGAAGCCCGAACGTCTACATTTGTTTGTACCAAGAATATCAAATCTTCGATTGTTTGTTTCAGCTGGATTCAGTTGCATATCGAAAGGAATCCGATGGTTTTGGACTGCCGGAGGCAGAACCAAGACAATTTTTAGTTATGGAATCTGCAGATGATAGAACAAGGTTAAGCACTGCAGCTACAACTCCATGTGAAGTTGATAATGCGGCCAAAGGATTAGGCAGCAGATCAGGCGGATTAGGCTTGTCTGATCAGTTGAATGAGTTGAGGACAGATCCAATTACCGCTCCAGGAGAAGCTAATATTTCTACTGTAAGAGAAAGATTAAGTAACTCTCAGGTATCTGATTGTAATGAAGCCTTTGAATTGGGTGCAAATGCAACCCGTGCCGAGGATTTTGCCGCCTGGGCGGCCACAGATGCTGCGCAGAATGTGCAGCCTACTGATAGACTAGAAGGATCACATTCTCAACCAGCAGATGATGCAAACGATTCAGCAACACAATCTCCGCCTATGCAGATGATGGAGCGGCCAGGCGACCCTGCCTCCTCACCGTATAGGATTCCGGATTATGTGTTTGCTAGGAACAAATCAACAGCCCCATTGGAATGGAGCACTGAATCCAATGAGTCCTTGTTCAGCATTCAGATGGGAAATACGAGTTTTACCAGAGACCAGTTTAACTGGTTGTGTAAATCTGGTGAATTGGGTTTACCTGGTGAGGTAAATTACTTGCCTGGTGGTCCATGCAACATTGACTTCACAAGCAACCAGCCTCCGGCTAAGCAAACTGCACAATCAGCGGAGAATGCTTCCAATAAGTTAAGCACGGTGGTGGAGGAGCCGAGTCTGATGAGGGTAACTGAAGAGAAAGCTGCGGAAACCATGAGAGAAGTTCTAAGGGAAAATGCAGTGAATCATGAGAAAGAGAAGTCAACCACCGCCCGTGAGGGCGGCAAGTATCACTCGGCTCGCCTCTCTCATGCTTCGGATGGAAGTACAAGATCTTTTGCATTCCCAATATTGTTAGGGGACATAAATGTTTCGCTACGGGGAGAAGGAACGAAACACAAGCACCAATTGGGGTCAACGAGAACGTCGCAGCGAGAATCGCAGCCGCAAACACCAAAAGAAACACCGAACGCGTCGTCTCCGGCCTCAAAACCAACCCCAATTGTACCCAAGTGCGGCTGGGGGCTGTCTTGCTTCTCTTGTTGCCCATCATGCTGTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

490

Amino Acids

53.25

Weight (kDa)

5.02

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Presenilin PF01080 2 - 57 5.3e-20 Presenilin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 195
Acc16I TGCGCA 1 cut(s) 660
Acc36I ACCTGC 1 cut(s) 195
AccBSI CCGCTC 2 cut(s) 521, 763
AccI GTMKAC 1 cut(s) 249
AccII CGCG 1 cut(s) 1379
AccIII TCCGGA 1 cut(s) 799
AclWI GGATC 3 cut(s) 228, 503, 700
AcoI YGGCCR 3 cut(s) 441, 645, 764
AcuI CTGAAG 1 cut(s) 1119
AfaI GTAC 6 cut(s) 129, 174, 263, 1234, 1413, 1465
AfiI CCNNNNNNNGG 3 cut(s) 625, 1012, 1421
AflIII ACRYGT 1 cut(s) 1377
AgsI TTSAA 2 cut(s) 493, 599
AjnI CCWGG 5 cut(s) 523, 638, 766, 949, 970
AleI CACNNNNGTG 1 cut(s) 1067
AluBI AGCT 7 cut(s) 77, 92, 101, 298, 413, 533, 1109
AluI AGCT 7 cut(s) 77, 92, 101, 298, 413, 533, 1109
Alw21I GWGCWC 1 cut(s) 851
Alw26I GTCTC 2 cut(s) 900, 1389
AlwI GGATC 3 cut(s) 228, 503, 700
AlwNI CAGNNNCTG 1 cut(s) 656
Aor13HI TCCGGA 1 cut(s) 799
AoxI GGCC 5 cut(s) 49, 441, 645, 764, 1389
ApeKI GCWGC 7 cut(s) 410, 456, 656, 670, 1109, 1336, 1348
ArsI GACNNNNNNTTYG 2 cut(s) 1367, 1399
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 1 cut(s) 977
AsuHPI GGTGA 3 cut(s) 777, 947, 965
AvaII GGWCC 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 851
BbvI GCAGC 7 cut(s) 422, 468, 643, 682, 1096, 1348, 1360
BccI CCATC 6 cut(s) 47, 323, 751, 877, 1220, 1462
BceAI ACGGC 1 cut(s) 164
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BciT130I CCWGG 5 cut(s) 525, 640, 768, 951, 972
BclI TGATCA 1 cut(s) 484
BcoDI GTCTC 2 cut(s) 900, 1389
BfaI CTAG 2 cut(s) 686, 816
BfmI CTRYAG 2 cut(s) 381, 408
BfuAI ACCTGC 1 cut(s) 195
BglI GCCNNNNNGGC 2 cut(s) 641, 644
BglII AGATCT 1 cut(s) 1238
BlpI GCTNAGC 1 cut(s) 1017
Bme1390I CCNGG 5 cut(s) 525, 640, 768, 951, 972
Bme18I GGWCC 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 2 cut(s) 227, 1077
BmrFI CCNGG 5 cut(s) 525, 640, 768, 951, 972
BmsI GCATC 3 cut(s) 200, 643, 706
BpmI CTGGAG 1 cut(s) 507
Bpu1102I GCTNAGC 1 cut(s) 1017
BsaI GGTCTC 1 cut(s) 900
BsaJI CCNNGG 4 cut(s) 52, 229, 624, 639
BsaWI WCCGGW 1 cut(s) 799
BsaXI ACNNNNNCTCC 4 cut(s) 724, 754, 764, 794
Bsc4I CCNNNNNNNGG 3 cut(s) 625, 1012, 1421
Bse1I ACTGG 3 cut(s) 162, 910, 923
BseAI TCCGGA 1 cut(s) 799
BseBI CCWGG 5 cut(s) 525, 640, 768, 951, 972
BseDI CCNNGG 4 cut(s) 52, 229, 624, 639
BseGI GGATG 2 cut(s) 215, 1231
BseLI CCNNNNNNNGG 3 cut(s) 625, 1012, 1421
BseMII CTCAG 1 cut(s) 584
BseNI ACTGG 3 cut(s) 162, 910, 923
BseRI GAGGAG 2 cut(s) 772, 1088
BseXI GCAGC 7 cut(s) 422, 468, 643, 682, 1096, 1348, 1360
BseYI CCCAGC 1 cut(s) 1425
BsgI GTGCAG 2 cut(s) 689, 1011
Bsh1236I CGCG 1 cut(s) 1379
BshFI GGCC 5 cut(s) 51, 443, 647, 766, 1391
BsiHKAI GWGCWC 1 cut(s) 851
BsiSI CCGG 4 cut(s) 344, 800, 1013, 1388
BslFI GGGAC 1 cut(s) 1280
BslI CCNNNNNNNGG 3 cut(s) 625, 1012, 1421
BsmAI GTCTC 2 cut(s) 900, 1389
BsmBI CGTCTC 1 cut(s) 1389
BsmFI GGGAC 1 cut(s) 1280
BsmI GAATGC 3 cut(s) 876, 1048, 1247
BsnI GGCC 5 cut(s) 51, 443, 647, 766, 1391
Bso31I GGTCTC 1 cut(s) 900
Bsp1286I GDGCHC 1 cut(s) 851
Bsp13I TCCGGA 1 cut(s) 799
Bsp1407I TGTACA 1 cut(s) 172
Bsp143I GATC 6 cut(s) 220, 461, 484, 508, 692, 1238
Bsp1720I GCTNAGC 1 cut(s) 1017
BspANI GGCC 5 cut(s) 51, 443, 647, 766, 1391
BspCNI CTCAG 1 cut(s) 583
BspEI TCCGGA 1 cut(s) 799
BspFNI CGCG 1 cut(s) 1379
BspHI TCATGA 1 cut(s) 1153
BspLI GGNNCC 2 cut(s) 227, 1077
BspMAI CTGCAG 2 cut(s) 385, 412
BspMI ACCTGC 1 cut(s) 195
BspPI GGATC 3 cut(s) 228, 503, 700
BspQI GCTCTTC 1 cut(s) 108
BspTNI GGTCTC 1 cut(s) 900
BsrBI CCGCTC 2 cut(s) 521, 763
BsrGI TGTACA 1 cut(s) 172
BsrI ACTGG 3 cut(s) 162, 910, 923
BssECI CCNNGG 4 cut(s) 52, 229, 624, 639
BssMI GATC 6 cut(s) 220, 461, 484, 508, 692, 1238
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 5 cut(s) 525, 640, 768, 951, 972
Bst4CI ACNGT 6 cut(s) 19, 72, 132, 547, 789, 1066
Bst6I CTCTTC 2 cut(s) 108, 1095
BstAPI GCANNNNNTGC 3 cut(s) 620, 667, 716
BstAUI TGTACA 1 cut(s) 172
BstC8I GCNNGC 2 cut(s) 99, 1207
BstDEI CTNAG 4 cut(s) 570, 1017, 1133, 1467
BstF5I GGATG 2 cut(s) 215, 1231
BstFNI CGCG 1 cut(s) 1379
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 6 cut(s) 223, 464, 487, 511, 695, 1241
BstMAI GTCTC 2 cut(s) 900, 1389
BstMBI GATC 6 cut(s) 220, 461, 484, 508, 692, 1238
BstNI CCWGG 5 cut(s) 525, 640, 768, 951, 972
BstSCI CCNGG 5 cut(s) 523, 638, 766, 949, 970
BstSFI CTRYAG 2 cut(s) 381, 408
BstUI CGCG 1 cut(s) 1379
BstV1I GCAGC 7 cut(s) 422, 468, 643, 682, 1096, 1348, 1360
BstX2I RGATCY 2 cut(s) 508, 1238
BstXI CCANNNNNNTGG 1 cut(s) 843
BstYI RGATCY 2 cut(s) 508, 1238
BsuRI GGCC 5 cut(s) 51, 443, 647, 766, 1391
BtsCI GGATG 2 cut(s) 215, 1231
BtsI GCAGTG 2 cut(s) 405, 1152
BtsIMutI CAGTG 3 cut(s) 405, 849, 1152
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 99, 1207
CaiI CAGNNNCTG 1 cut(s) 656
CciI TCATGA 1 cut(s) 1153
CfoI GCGC 1 cut(s) 661
Cfr13I GGNCC 1 cut(s) 977
CseI GACGC 1 cut(s) 1368
CsiI ACCWGGT 1 cut(s) 949
Csp6I GTAC 6 cut(s) 128, 173, 262, 1233, 1412, 1464
CviAII CATG 6 cut(s) 423, 981, 1120, 1154, 1217, 1458
CviQI GTAC 6 cut(s) 128, 173, 262, 1233, 1412, 1464
DdeI CTNAG 4 cut(s) 570, 1017, 1133, 1467
DpnI GATC 6 cut(s) 222, 463, 486, 510, 694, 1240
DpnII GATC 6 cut(s) 220, 461, 484, 508, 692, 1238
EaeI YGGCCR 3 cut(s) 441, 645, 764
Eam1104I CTCTTC 2 cut(s) 108, 1095
EarI CTCTTC 2 cut(s) 108, 1095
EciI GGCGGA 2 cut(s) 483, 732
Eco130I CCWWGG 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 900
Eco47I GGWCC 1 cut(s) 977
Eco57I CTGAAG 1 cut(s) 1119
EcoRII CCWGG 5 cut(s) 523, 638, 766, 949, 970
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 1389
FaeI CATG 6 cut(s) 426, 984, 1123, 1157, 1220, 1461
FaqI GGGAC 1 cut(s) 1280
FatI CATG 6 cut(s) 422, 980, 1119, 1153, 1216, 1457
FbaI TGATCA 1 cut(s) 484
FblI GTMKAC 1 cut(s) 249
FokI GGATG 2 cut(s) 222, 1238
FspBI CTAG 2 cut(s) 686, 816
FspI TGCGCA 1 cut(s) 660
GlaI GCGC 1 cut(s) 660
GsaI CCCAGC 1 cut(s) 1429
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 5 cut(s) 51, 443, 647, 766, 1391
HapII CCGG 4 cut(s) 344, 800, 1013, 1388
HgaI GACGC 1 cut(s) 1368
HhaI GCGC 1 cut(s) 661
Hin1II CATG 6 cut(s) 426, 984, 1123, 1157, 1220, 1461
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HincII GTYRAC 2 cut(s) 1170, 1323
HindII GTYRAC 2 cut(s) 1170, 1323
HindIII AAGCTT 1 cut(s) 90
HpaII CCGG 4 cut(s) 344, 800, 1013, 1388
HphI GGTGA 3 cut(s) 777, 947, 965
Hpy166II GTNNAC 4 cut(s) 250, 947, 1170, 1323
Hpy188III TCNNGA 2 cut(s) 800, 1154
Hpy8I GTNNAC 4 cut(s) 250, 947, 1170, 1323
Hpy99I CGWCG 2 cut(s) 1336, 1384
HpyAV CCTTC 3 cut(s) 196, 683, 1286
HpyCH4III ACNGT 6 cut(s) 19, 72, 132, 547, 789, 1066
HpyCH4IV ACGT 2 cut(s) 246, 1331
HpyF3I CTNAG 4 cut(s) 570, 1017, 1133, 1467
HpySE526I ACGT 2 cut(s) 246, 1331
Hsp92II CATG 6 cut(s) 426, 984, 1123, 1157, 1220, 1461
HspAI GCGC 1 cut(s) 659
Kpn2I TCCGGA 1 cut(s) 799
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 6 cut(s) 220, 461, 484, 508, 692, 1238
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 5 cut(s) 225, 526, 760, 846, 1075
Lsp1109I GCAGC 7 cut(s) 422, 468, 643, 682, 1096, 1348, 1360
LweI GCATC 3 cut(s) 200, 643, 706
MabI ACCWGGT 1 cut(s) 949
MaeI CTAG 2 cut(s) 686, 816
MaeII ACGT 2 cut(s) 246, 1331
MaeIII GTNAC 3 cut(s) 58, 563, 1093
MalI GATC 6 cut(s) 222, 463, 486, 510, 694, 1240
MbiI CCGCTC 2 cut(s) 521, 763
MboI GATC 6 cut(s) 220, 461, 484, 508, 692, 1238
MboII GAAGA 3 cut(s) 95, 271, 1112
MfeI CAATTG 2 cut(s) 1313, 1407
MflI RGATCY 2 cut(s) 508, 1238
MhlI GDGCHC 1 cut(s) 851
MluCI AATT 8 cut(s) 176, 362, 513, 599, 938, 961, 1313, 1407
MluI ACGCGT 1 cut(s) 1377
MlyI GAGTC 2 cut(s) 872, 1090
MmeI TCCRAC 1 cut(s) 205
MroI TCCGGA 1 cut(s) 799
MseI TTAA 4 cut(s) 401, 560, 915, 1058
MslI CAYNNNNRTG 3 cut(s) 841, 881, 1067
MspA1I CMGCKG 2 cut(s) 298, 1037
MspI CCGG 4 cut(s) 344, 800, 1013, 1388
MspR9I CCNGG 5 cut(s) 525, 640, 768, 951, 972
MunI CAATTG 2 cut(s) 1313, 1407
Mva1269I GAATGC 3 cut(s) 876, 1048, 1247
MvaI CCWGG 5 cut(s) 525, 640, 768, 951, 972
MvnI CGCG 1 cut(s) 1379
NdeII GATC 6 cut(s) 220, 461, 484, 508, 692, 1238
NlaIII CATG 6 cut(s) 426, 984, 1123, 1157, 1220, 1461
NlaIV GGNNCC 2 cut(s) 227, 1077
NmeAIII GCCGAG 4 cut(s) 102, 649, 1104, 1181
NsbI TGCGCA 1 cut(s) 660
OliI CACNNNNGTG 1 cut(s) 1067
PagI TCATGA 1 cut(s) 1153
PaqCI CACCTGC 1 cut(s) 195
PciSI GCTCTTC 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 3 cut(s) 876, 1048, 1247
PfeI GAWTC 8 cut(s) 302, 323, 377, 725, 796, 854, 1150, 1343
PfoI TCCNGGA 1 cut(s) 523
PleI GAGTC 2 cut(s) 871, 1089
PpsI GAGTC 2 cut(s) 871, 1089
Psp6I CCWGG 5 cut(s) 523, 638, 766, 949, 970
PspFI CCCAGC 1 cut(s) 1425
PspGI CCWGG 5 cut(s) 523, 638, 766, 949, 970
PspN4I GGNNCC 2 cut(s) 227, 1077
PspPI GGNCC 1 cut(s) 977
PstI CTGCAG 2 cut(s) 385, 412
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 2 cut(s) 508, 1238
PvuII CAGCTG 1 cut(s) 298
RsaI GTAC 6 cut(s) 129, 174, 263, 1234, 1413, 1465
RsaNI GTAC 6 cut(s) 128, 173, 262, 1233, 1412, 1464
RseI CAYNNNNRTG 3 cut(s) 841, 881, 1067
SapI GCTCTTC 1 cut(s) 108
SaqAI TTAA 4 cut(s) 401, 560, 915, 1058
Sau3AI GATC 6 cut(s) 220, 461, 484, 508, 692, 1238
Sau96I GGNCC 1 cut(s) 977
SchI GAGTC 2 cut(s) 872, 1090
ScrFI CCNGG 5 cut(s) 525, 640, 768, 951, 972
SduI GDGCHC 1 cut(s) 851
SexAI ACCWGGT 1 cut(s) 949
SfaNI GCATC 3 cut(s) 200, 643, 706
SfcI CTRYAG 2 cut(s) 381, 408
SinI GGWCC 1 cut(s) 977
SmiMI CAYNNNNRTG 3 cut(s) 841, 881, 1067
Sse9I AATT 8 cut(s) 176, 362, 513, 599, 938, 961, 1313, 1407
SspI AATATT 2 cut(s) 538, 1257
SspMI CTAG 2 cut(s) 686, 816
StyD4I CCNGG 5 cut(s) 523, 638, 766, 949, 970
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 6 cut(s) 19, 72, 132, 547, 789, 1066
TaiI ACGT 2 cut(s) 249, 1334
TaqI TCGA 2 cut(s) 282, 316
TasI AATT 8 cut(s) 176, 362, 513, 599, 938, 961, 1313, 1407
TatI WGTACW 3 cut(s) 172, 1232, 1463
TauI GCSGC 7 cut(s) 443, 638, 647, 766, 1191, 1354, 1425
TfiI GAWTC 8 cut(s) 302, 323, 377, 725, 796, 854, 1150, 1343
Tru1I TTAA 4 cut(s) 401, 560, 915, 1058
Tru9I TTAA 4 cut(s) 401, 560, 915, 1058
TscAI CASTG 3 cut(s) 412, 856, 1152
TseI GCWGC 7 cut(s) 410, 456, 656, 670, 1109, 1336, 1348
TspDTI ATGAA 1 cut(s) 603
TspRI CASTG 3 cut(s) 412, 856, 1152
VpaK11BI GGWCC 1 cut(s) 977
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 2 cut(s) 686, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.