pycom14g19140

Mitochondrial ribosomal subunit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006027
Physical Location & Seq
Reverse (-)
20562593 .. 20564551
1959 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1260 bp
ATGAGACGAACTCTGCTGAGCAATCTCTCTCTCTGCACTCGCAATCGCCTTCTCTCTCCTCAAACATTCATCCCAAATCCTACCCCCTCACTCGTCCCACTCGCCCGTTCGACTCGGTCCCTGCTCAGCCGGTTCTTCTCCTCAGACGGCGACTCCTCCGCCGAAGGCCCCAATCCGGCTGCTGATTCGAGCTTGATCCCAACCAAAAAGAAAGATGCCTCCGTCGAAGTCAAGGACGTCAATAACAAAGAGCTGAAGGAGCGGATAAATAAATATTTTAAGGGTGATGAGGAGGCGCTCCCTGAGATACTTGAAGCGATTCTGCAGAGGAGATTGACTGGGAAGCACGAGGAAACAGATGATGAGCTGATAGATGAATTGCAAATGCAGCCATTGGATGATGTTAAGGACCAAGAGTTTGAATCGGATTTTGAGGAATTGTATGACACGGATGAAGAGATTGATGACTTGTATAATGCAAAGGATATGGTCATGAATAGGATGTCAAAAGATGAGTACTTCAACATGGATGCCAAGAAGTGGGATGATATTGTTGAGGATGCAATTAAGCACGGGATTATGAAGGATACAAAGGAGTGTGAGGCGATTCTCGAGGATATGCTTAGCTGGGATAAGCTCCTGCCAGATGACATGAAAAGGAAGGTAGAAGAGAAGTTCAATGACTTAGGGGATAAGTGTGAAAGAGGGGAGCTTCAACCTGAAGAAGCTTATGAGTTGTTCAAGGAGTTTGAGGATGAGATTGTCATGGACTATTTGCAGAAGATGGAAGCCGACGGCCCCCCACAATTTGATGAGGCTGATGTGCCTGACAAGAAGGATCTAGCCGACCCACCAGGCGAAGGGCCAATCCTTAGGTGGCAAACACGGGTAGTCTTTGCTCCTGGTGGTGATTCCTGGCATCCCAAGAATAGAAAAGTGAAACTGTCTGTTACAGTGAAAGAACTCGGGCTTTCAAAGCATCAATTCCGTCGGCTCAGAGACTTGGTTGGAAAGCGGTACAATCCAGGGAAAGATGAGCTTACAATTACTAGTGAGAGGTTTGAACACCGAGAAGAAAATAGAAAGGATTGCCTTAGGACACTTCTTTCCCTTATTGAGGAAGCTGGGAAGGCTAACAAACTGGCTGAGGATGCTCGAGTTTCGTATGTCAAGGAGAGATTAAGAGCAAATCCCGCATTCATGGAGAGGCTGCACAACAAGACCATGAGATTGCAAGGAAAGAGCGCAGTACCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S28

Protein Analysis

420

Amino Acids

48.4

Weight (kDa)

4.94

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S28 PF10213 305 - 384 3.4e-17 Mitochondrial ribosomal subunit protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 240
AccB7I CCANNNNNTGG 1 cut(s) 540
AccBSI CCGCTC 1 cut(s) 262
AciI CCGC 4 cut(s) 159, 262, 1015, 1194
AclWI GGATC 2 cut(s) 190, 846
AcuI CTGAAG 2 cut(s) 275, 741
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 3 cut(s) 518, 1019, 1251
AfiI CCNNNNNNNGG 4 cut(s) 175, 540, 860, 1117
AgsI TTSAA 8 cut(s) 314, 422, 523, 679, 716, 742, 975, 1064
AhlI ACTAGT 1 cut(s) 1049
AjnI CCWGG 4 cut(s) 853, 901, 914, 1024
AluBI AGCT 9 cut(s) 192, 253, 367, 627, 637, 712, 728, 1039, 1124
AluI AGCT 9 cut(s) 192, 253, 367, 627, 637, 712, 728, 1039, 1124
Alw26I GTCTC 1 cut(s) 993
AlwI GGATC 2 cut(s) 190, 846
AlwNI CAGNNNCTG 1 cut(s) 1253
Ama87I CYCGRG 3 cut(s) 611, 965, 1155
AoxI GGCC 3 cut(s) 166, 796, 863
ApeKI GCWGC 3 cut(s) 179, 388, 1210
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 2 cut(s) 298, 1247
AspS9I GGNCC 5 cut(s) 117, 167, 409, 797, 863
AsuHPI GGTGA 2 cut(s) 296, 920
AvaI CYCGRG 3 cut(s) 611, 965, 1155
AvaII GGWCC 2 cut(s) 117, 409
AxyI CCTNAGG 2 cut(s) 872, 1094
BauI CACGAG 1 cut(s) 347
BbvCI CCTCAGC 1 cut(s) 1146
BbvI GCAGC 3 cut(s) 166, 400, 1197
BccI CCATC 1 cut(s) 778
BceAI ACGGC 2 cut(s) 163, 811
BciT130I CCWGG 4 cut(s) 855, 903, 916, 1026
BciVI GTATCC 1 cut(s) 580
BcoDI GTCTC 1 cut(s) 993
BcuI ACTAGT 1 cut(s) 1049
BfaI CTAG 2 cut(s) 842, 1050
BfmI CTRYAG 1 cut(s) 323
BfoI RGCGCY 1 cut(s) 299
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 3 cut(s) 180, 389, 1211
BlpI GCTNAGC 3 cut(s) 17, 125, 623
BlsI GCNGC 3 cut(s) 181, 390, 1212
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 4 cut(s) 855, 903, 916, 1026
Bme18I GGWCC 2 cut(s) 117, 409
BmeT110I CYCGRG 3 cut(s) 611, 965, 1155
BmgT120I GGNCC 5 cut(s) 117, 167, 409, 797, 863
BmiI GGNNCC 3 cut(s) 119, 169, 799
BmrFI CCNGG 4 cut(s) 855, 903, 916, 1026
BmrI ACTGGG 1 cut(s) 348
BmsI GCATC 6 cut(s) 205, 520, 550, 928, 988, 1141
BmuI ACTGGG 1 cut(s) 348
BplI GAGNNNNNCTC 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 1146
Bpu1102I GCTNAGC 3 cut(s) 17, 125, 623
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 1025
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 540, 860, 1117
Bse118I RCCGGY 1 cut(s) 129
Bse1I ACTGG 2 cut(s) 343, 1146
Bse21I CCTNAGG 2 cut(s) 872, 1094
BseBI CCWGG 4 cut(s) 855, 903, 916, 1026
BseDI CCNNGG 1 cut(s) 1025
BseLI CCNNNNNNNGG 4 cut(s) 175, 540, 860, 1117
BseMII CTCAG 6 cut(s) 8, 139, 156, 294, 1009, 1137
BseNI ACTGG 2 cut(s) 343, 1146
BseRI GAGGAG 5 cut(s) 48, 130, 145, 305, 343
BseXI GCAGC 3 cut(s) 166, 400, 1197
BseYI CCCAGC 2 cut(s) 627, 1124
BsgI GTGCAG 2 cut(s) 19, 1196
BshFI GGCC 3 cut(s) 168, 798, 865
BsiHKCI CYCGRG 3 cut(s) 611, 965, 1155
BsiSI CCGG 2 cut(s) 130, 176
BslFI GGGAC 2 cut(s) 80, 103
BslI CCNNNNNNNGG 4 cut(s) 175, 540, 860, 1117
BsmAI GTCTC 1 cut(s) 993
BsmFI GGGAC 2 cut(s) 80, 103
BsmI GAATGC 1 cut(s) 1196
BsnI GGCC 3 cut(s) 168, 798, 865
BsoBI CYCGRG 3 cut(s) 611, 965, 1155
Bsp143I GATC 2 cut(s) 195, 838
Bsp1720I GCTNAGC 3 cut(s) 17, 125, 623
BspACI CCGC 4 cut(s) 159, 262, 1015, 1194
BspANI GGCC 3 cut(s) 168, 798, 865
BspCNI CTCAG 6 cut(s) 9, 138, 155, 295, 1008, 1138
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 3 cut(s) 119, 169, 799
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 2 cut(s) 190, 846
BsrBI CCGCTC 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 129
BsrI ACTGG 2 cut(s) 343, 1146
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 1025
BssMI GATC 2 cut(s) 195, 838
BssNI GRCGYC 1 cut(s) 237
BssSI CACGAG 1 cut(s) 347
Bst2BI CACGAG 1 cut(s) 347
Bst2UI CCWGG 4 cut(s) 855, 903, 916, 1026
Bst4CI ACNGT 2 cut(s) 945, 955
Bst6I CTCTTC 2 cut(s) 450, 663
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstENI CCTNNNNNAGG 1 cut(s) 1115
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 2 cut(s) 298, 1247
BstKTI GATC 2 cut(s) 198, 841
BstMAI GTCTC 1 cut(s) 993
BstMBI GATC 2 cut(s) 195, 838
BstMWI GCNNNNNNNGC 7 cut(s) 259, 388, 976, 1130, 1151, 1193, 1253
BstNI CCWGG 4 cut(s) 855, 903, 916, 1026
BstSCI CCNGG 4 cut(s) 853, 901, 914, 1024
BstSFI CTRYAG 1 cut(s) 323
BstV1I GCAGC 3 cut(s) 166, 400, 1197
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
Bsu36I CCTNAGG 2 cut(s) 872, 1094
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 3 cut(s) 168, 798, 865
BtsIMutI CAGTG 1 cut(s) 960
CaiI CAGNNNCTG 1 cut(s) 1253
CciI TCATGA 1 cut(s) 492
CfoI GCGC 2 cut(s) 298, 1247
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 5 cut(s) 117, 167, 409, 797, 863
Csp6I GTAC 3 cut(s) 517, 1018, 1250
CviAII CATG 6 cut(s) 493, 526, 652, 766, 1201, 1225
CviQI GTAC 3 cut(s) 517, 1018, 1250
DpnI GATC 2 cut(s) 197, 840
DpnII GATC 2 cut(s) 195, 838
Eam1104I CTCTTC 2 cut(s) 450, 663
EarI CTCTTC 2 cut(s) 450, 663
EciI GGCGGA 1 cut(s) 148
Eco47I GGWCC 2 cut(s) 117, 409
Eco57I CTGAAG 2 cut(s) 275, 741
Eco81I CCTNAGG 2 cut(s) 872, 1094
Eco88I CYCGRG 3 cut(s) 611, 965, 1155
EcoNI CCTNNNNNAGG 1 cut(s) 1115
EcoO109I RGGNCCY 1 cut(s) 167
EcoRII CCWGG 4 cut(s) 853, 901, 914, 1024
FaeI CATG 6 cut(s) 496, 529, 655, 769, 1204, 1228
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FaqI GGGAC 2 cut(s) 80, 103
FatI CATG 6 cut(s) 492, 525, 651, 765, 1200, 1224
FauI CCCGC 1 cut(s) 1201
Fnu4HI GCNGC 3 cut(s) 180, 389, 1211
Fsp4HI GCNGC 3 cut(s) 180, 389, 1211
FspBI CTAG 2 cut(s) 842, 1050
GlaI GCGC 2 cut(s) 297, 1246
GluI GCNGC 3 cut(s) 180, 389, 1211
GsaI CCCAGC 2 cut(s) 631, 1128
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 3 cut(s) 168, 798, 865
HapII CCGG 2 cut(s) 130, 176
HhaI GCGC 2 cut(s) 298, 1247
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 6 cut(s) 496, 529, 655, 769, 1204, 1228
Hin6I GCGC 2 cut(s) 296, 1245
HinP1I GCGC 2 cut(s) 296, 1245
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 7 cut(s) 112, 152, 185, 319, 422, 607, 911
HpaII CCGG 2 cut(s) 130, 176
HphI GGTGA 2 cut(s) 296, 920
Hpy188I TCNGA 3 cut(s) 145, 427, 998
Hpy188III TCNNGA 2 cut(s) 493, 611
Hpy99I CGWCG 3 cut(s) 227, 797, 993
HpyAV CCTTC 8 cut(s) 59, 158, 250, 577, 655, 829, 854, 1123
HpyCH4III ACNGT 2 cut(s) 945, 955
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 9 cut(s) 36, 325, 382, 388, 479, 563, 778, 1213, 1234
HpyF10VI GCNNNNNNNGC 7 cut(s) 259, 388, 976, 1130, 1151, 1193, 1253
HpySE526I ACGT 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 6 cut(s) 496, 529, 655, 769, 1204, 1228
HspAI GCGC 2 cut(s) 296, 1245
Kzo9I GATC 2 cut(s) 195, 838
LmnI GCTCC 5 cut(s) 259, 303, 642, 709, 904
Lsp1109I GCAGC 3 cut(s) 166, 400, 1197
LweI GCATC 6 cut(s) 205, 520, 550, 928, 988, 1141
MaeI CTAG 2 cut(s) 842, 1050
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 949
MalI GATC 2 cut(s) 197, 840
MbiI CCGCTC 1 cut(s) 262
MboI GATC 2 cut(s) 195, 838
MboII GAAGA 6 cut(s) 127, 467, 680, 734, 793, 1085
MflI RGATCY 1 cut(s) 838
MluCI AATT 6 cut(s) 377, 437, 564, 806, 983, 1044
MlyI GAGTC 2 cut(s) 106, 146
MmeI TCCRAC 1 cut(s) 988
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 4 cut(s) 279, 405, 567, 1181
MspI CCGG 2 cut(s) 130, 176
MspR9I CCNGG 4 cut(s) 855, 903, 916, 1026
Mva1269I GAATGC 1 cut(s) 1196
MvaI CCWGG 4 cut(s) 855, 903, 916, 1026
MwoI GCNNNNNNNGC 7 cut(s) 259, 388, 976, 1130, 1151, 1193, 1253
NdeII GATC 2 cut(s) 195, 838
NlaIII CATG 6 cut(s) 496, 529, 655, 769, 1204, 1228
NlaIV GGNNCC 3 cut(s) 119, 169, 799
PaeR7I CTCGAG 2 cut(s) 611, 1155
PagI TCATGA 1 cut(s) 492
PctI GAATGC 1 cut(s) 1196
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 5 cut(s) 185, 319, 422, 607, 911
PflFI GACNNNGTC 1 cut(s) 115
PflMI CCANNNNNTGG 1 cut(s) 540
PkrI GCNGC 3 cut(s) 181, 390, 1212
PleI GAGTC 2 cut(s) 106, 146
PpsI GAGTC 2 cut(s) 106, 146
Psp6I CCWGG 4 cut(s) 853, 901, 914, 1024
PspFI CCCAGC 2 cut(s) 627, 1124
PspGI CCWGG 4 cut(s) 853, 901, 914, 1024
PspN4I GGNNCC 3 cut(s) 119, 169, 799
PspPI GGNCC 5 cut(s) 117, 167, 409, 797, 863
PspXI VCTCGAGB 1 cut(s) 1155
PstI CTGCAG 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 1253
PsuI RGATCY 1 cut(s) 838
PsyI GACNNNGTC 1 cut(s) 115
RsaI GTAC 3 cut(s) 518, 1019, 1251
RsaNI GTAC 3 cut(s) 517, 1018, 1250
SaqAI TTAA 4 cut(s) 279, 405, 567, 1181
SatI GCNGC 3 cut(s) 180, 389, 1211
Sau3AI GATC 2 cut(s) 195, 838
Sau96I GGNCC 5 cut(s) 117, 167, 409, 797, 863
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 2 cut(s) 106, 146
ScrFI CCNGG 4 cut(s) 855, 903, 916, 1026
SfaNI GCATC 6 cut(s) 205, 520, 550, 928, 988, 1141
SfcI CTRYAG 1 cut(s) 323
Sfr274I CTCGAG 2 cut(s) 611, 1155
SinI GGWCC 2 cut(s) 117, 409
SlaI CTCGAG 2 cut(s) 611, 1155
SmlI CTYRAG 2 cut(s) 611, 1155
SmoI CTYRAG 2 cut(s) 611, 1155
SpeI ACTAGT 1 cut(s) 1049
Sse9I AATT 6 cut(s) 377, 437, 564, 806, 983, 1044
SsiI CCGC 4 cut(s) 159, 262, 1015, 1194
SspI AATATT 1 cut(s) 275
SspMI CTAG 2 cut(s) 842, 1050
StyD4I CCNGG 4 cut(s) 853, 901, 914, 1024
TaaI ACNGT 2 cut(s) 945, 955
TaiI ACGT 1 cut(s) 240
TaqI TCGA 5 cut(s) 110, 188, 225, 612, 1156
TaqII GACCGA 1 cut(s) 105
TasI AATT 6 cut(s) 377, 437, 564, 806, 983, 1044
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 5 cut(s) 185, 319, 422, 607, 911
Tru1I TTAA 4 cut(s) 279, 405, 567, 1181
Tru9I TTAA 4 cut(s) 279, 405, 567, 1181
TscAI CASTG 1 cut(s) 960
TseI GCWGC 3 cut(s) 179, 388, 1210
TspDTI ATGAA 7 cut(s) 58, 390, 468, 509, 596, 668, 1189
TspGWI ACGGA 3 cut(s) 211, 464, 977
TspRI CASTG 1 cut(s) 960
Tth111I GACNNNGTC 1 cut(s) 115
Van91I CCANNNNNTGG 1 cut(s) 540
VpaK11BI GGWCC 2 cut(s) 117, 409
XagI CCTNNNNNAGG 1 cut(s) 1115
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XhoI CTCGAG 2 cut(s) 611, 1155
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 2 cut(s) 842, 1050
ZraI GACGTC 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.