pycom14g19170

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011787
Physical Location & Seq
Reverse (-)
20583083 .. 20584758
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
ATGTTCGAAGAATACGACCGCACCTCCTCCGCCTCCCCCCTCAAGCCTTCCCGCCTCCGATTGTTTCTCTTCTTCGCGAAGCCGGAAACCGCCGCCACGATGGGTGCTCTGCTCGACGACGCAAAGTCCGAGTCTTGGTTCGTCGACGCGCTGAACGGGTCGGGTTTTCCGAGGAACCTGTCGGATTCCGCCACTATGGATTACTTGCTGGCGGGGAGTGAGAATTCTTGCAATGATTTGGAGGCGCAAGGCGACGTCTTCGGAAGCAACAACAAACAGGACAACAACAATGGTTTGATGAAAAATGTTAACAATGTGGTCGGACATGATGTTCATTCGATCAATCAAGAGTCCACCTTTGCCGAGGACAGTACTTCCTTTTTCGGGTTGTCTTCTTCATCGACTTGCTCGTCGAATTTGCCTCCGATTCGGGTTCGAACGGACGAAACTGGTGCTAGGTTGATGCAGGGTGGTCAGAGGACTGTTGGGATCGAGGAGCAGTTTGCTCAGATGAGTACTAGTTATGCTGCTGTTGTTGCTGCTCCGCCGCCAATGGCGTCGAATTCGGGTGCTTTGCCGCCACCTGGTGCTCAGATGATGAATGTTTCTGGCGTTCCTGGAGAAAATATGAACAGGGTAGATGATGAGAGATCACCAGATCAGAGTATACCCGTCGGATTTCGTAAGCCTCCTCTGCCATTGCCGGTGCAGCCTCTACAAGTTAATAAGGCTGGCGGCGGCGGAGGAGGTTATAGTTTGCCTTCACCGGATTCAGTTGCAAGTGATAGTAGCATTGCATCCGCAAACTCTGTCTCCAAACCAATGTACTATCAAGAGCAGATTCAAGCTGCAAACAGGGACAACATCAGAGGTCCTGCTAGCCCGAATACACCCAGTGATGCTTCCGATACGGGATCGATCAAAACCAAGTTCAGCAAGTTCAGGATCCCGGGTACCAACAACTACAACAGCAGCAGCAGCAGCAACAACAACAATATGTCCATGCCAGCACACATTACATGCACCATCCTGCCGGAGGCCAAGTGCCAATGCAATCTTACTACACAATGTATGCACCCCCGTCACAGCAACAACCTCATCACCAAATCGATCACCAGCAGTATCCAGTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

379

Amino Acids

40.24

Weight (kDa)

5.31

Isoelectric Point (pI)

55.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 409
AatII GACGTC 1 cut(s) 258
Acc65I GGTACC 1 cut(s) 953
AccB1I GGYRCC 1 cut(s) 953
AccI GTMKAC 2 cut(s) 144, 667
AccII CGCG 2 cut(s) 77, 149
AclWI GGATC 4 cut(s) 497, 922, 940, 953
AcsI RAATTY 3 cut(s) 223, 415, 562
AcyI GRCGYC 2 cut(s) 255, 557
AdeI CACNNNGTG 2 cut(s) 587, 896
AfaI GTAC 4 cut(s) 373, 517, 827, 955
AfiI CCNNNNNNNGG 4 cut(s) 135, 384, 584, 1036
AgsI TTSAA 1 cut(s) 845
AhdI GACNNNNNGTC 1 cut(s) 124
AhlI ACTAGT 1 cut(s) 518
AjnI CCWGG 2 cut(s) 583, 616
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 1 cut(s) 848
AluI AGCT 1 cut(s) 848
Alw21I GWGCWC 2 cut(s) 109, 592
Alw26I GTCTC 1 cut(s) 817
AlwI GGATC 4 cut(s) 497, 922, 940, 953
Ama87I CYCGRG 1 cut(s) 949
AoxI GGCC 1 cut(s) 1038
ApeKI GCWGC 8 cut(s) 527, 539, 709, 848, 972, 975, 978, 981
ApoI RAATTY 3 cut(s) 223, 415, 562
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
Asp718I GGTACC 1 cut(s) 953
AspLEI GCGC 2 cut(s) 151, 247
AspS9I GGNCC 1 cut(s) 872
AsuC2I CCSGG 2 cut(s) 950, 951
AsuHPI GGTGA 4 cut(s) 645, 756, 1093, 1105
AsuII TTCGAA 2 cut(s) 6, 436
AsuNHI GCTAGC 1 cut(s) 878
AvaI CYCGRG 1 cut(s) 949
AvaII GGWCC 1 cut(s) 872
BamHI GGATCC 1 cut(s) 945
BanI GGYRCC 1 cut(s) 953
BbsI GAAGAC 2 cut(s) 250, 384
Bbv12I GWGCWC 2 cut(s) 109, 592
BbvI GCAGC 8 cut(s) 514, 526, 721, 835, 984, 987, 990, 993
BccI CCATC 2 cut(s) 94, 1034
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 2 cut(s) 585, 618
BciVI GTATCC 1 cut(s) 1133
BcnI CCSGG 2 cut(s) 950, 951
BcoDI GTCTC 1 cut(s) 817
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 3 cut(s) 456, 519, 879
BfuI GTATCC 1 cut(s) 1133
BmcAI AGTACT 2 cut(s) 373, 517
Bme1390I CCNGG 4 cut(s) 585, 618, 950, 951
Bme18I GGWCC 1 cut(s) 872
BmeRI GACNNNNNGTC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 949
BmgT120I GGNCC 1 cut(s) 872
BmiI GGNNCC 3 cut(s) 176, 947, 955
BmrFI CCNGG 4 cut(s) 585, 618, 950, 951
BmrI ACTGGG 1 cut(s) 888
BmsI GCATC 3 cut(s) 453, 806, 889
BmtI GCTAGC 1 cut(s) 882
BmuI ACTGGG 1 cut(s) 888
BpiI GAAGAC 2 cut(s) 250, 384
BpmI CTGGAG 1 cut(s) 639
Bpu14I TTCGAA 2 cut(s) 6, 436
BpuEI CTTGAG 1 cut(s) 26
BpuMI CCSGG 2 cut(s) 950, 951
Bsa29I ATCGAT 2 cut(s) 917, 1109
BsaHI GRCGYC 2 cut(s) 255, 557
BsaJI CCNNGG 3 cut(s) 170, 363, 949
BsaWI WCCGGW 1 cut(s) 766
BsaXI ACNNNNNCTCC 6 cut(s) 8, 38, 797, 827, 1028, 1058
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 384, 584, 1036
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 3 cut(s) 454, 894, 1126
Bse3DI GCAATG 3 cut(s) 238, 698, 792
BseBI CCWGG 2 cut(s) 585, 618
BseCI ATCGAT 2 cut(s) 917, 1109
BseDI CCNNGG 3 cut(s) 170, 363, 949
BseGI GGATG 2 cut(s) 797, 1026
BseLI CCNNNNNNNGG 4 cut(s) 135, 384, 584, 1036
BseMI GCAATG 3 cut(s) 238, 698, 792
BseMII CTCAG 2 cut(s) 521, 605
BseNI ACTGG 3 cut(s) 454, 894, 1126
BseRI GAGGAG 4 cut(s) 16, 509, 681, 759
BseXI GCAGC 8 cut(s) 514, 526, 721, 835, 984, 987, 990, 993
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 2 cut(s) 77, 149
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 1 cut(s) 1040
BshNI GGYRCC 1 cut(s) 953
BshVI ATCGAT 2 cut(s) 917, 1109
BsiEI CGRYCG 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 109, 592
BsiHKCI CYCGRG 1 cut(s) 949
BsiSI CCGG 5 cut(s) 83, 704, 767, 950, 1034
BslFI GGGAC 1 cut(s) 872
BslI CCNNNNNNNGG 4 cut(s) 135, 384, 584, 1036
BsmAI GTCTC 1 cut(s) 817
BsmFI GGGAC 1 cut(s) 872
BsnI GGCC 1 cut(s) 1040
BsoBI CYCGRG 1 cut(s) 949
Bsp119I TTCGAA 2 cut(s) 6, 436
Bsp1286I GDGCHC 2 cut(s) 109, 592
Bsp143I GATC 8 cut(s) 339, 489, 650, 658, 914, 918, 945, 1110
Bsp68I TCGCGA 1 cut(s) 77
BspANI GGCC 1 cut(s) 1040
BspCNI CTCAG 2 cut(s) 520, 604
BspDI ATCGAT 2 cut(s) 917, 1109
BspFNI CGCG 2 cut(s) 77, 149
BspLI GGNNCC 3 cut(s) 176, 947, 955
BspOI GCTAGC 1 cut(s) 882
BspPI GGATC 4 cut(s) 497, 922, 940, 953
BspT104I TTCGAA 2 cut(s) 6, 436
BspT107I GGYRCC 1 cut(s) 953
BsrDI GCAATG 3 cut(s) 238, 698, 792
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 3 cut(s) 454, 894, 1126
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 3 cut(s) 170, 363, 949
BssMI GATC 8 cut(s) 339, 489, 650, 658, 914, 918, 945, 1110
BssNAI GTATAC 1 cut(s) 668
BssNI GRCGYC 2 cut(s) 255, 557
Bst1107I GTATAC 1 cut(s) 668
Bst2UI CCWGG 2 cut(s) 585, 618
Bst4CI ACNGT 2 cut(s) 371, 484
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 2 cut(s) 255, 557
BstBI TTCGAA 2 cut(s) 6, 436
BstC8I GCNNGC 4 cut(s) 210, 733, 880, 1008
BstDEI CTNAG 2 cut(s) 507, 591
BstENI CCTNNNNNAGG 1 cut(s) 1034
BstF5I GGATG 2 cut(s) 797, 1026
BstFNI CGCG 2 cut(s) 77, 149
BstHHI GCGC 2 cut(s) 151, 247
BstKTI GATC 8 cut(s) 342, 492, 653, 661, 917, 921, 948, 1113
BstMAI GTCTC 1 cut(s) 817
BstMBI GATC 8 cut(s) 339, 489, 650, 658, 914, 918, 945, 1110
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 5 cut(s) 536, 694, 709, 978, 981
BstNI CCWGG 2 cut(s) 585, 618
BstNSI RCATGY 1 cut(s) 1023
BstSCI CCNGG 4 cut(s) 583, 616, 948, 949
BstUI CGCG 2 cut(s) 77, 149
BstV1I GCAGC 8 cut(s) 514, 526, 721, 835, 984, 987, 990, 993
BstV2I GAAGAC 2 cut(s) 250, 384
BstX2I RGATCY 1 cut(s) 945
BstYI RGATCY 1 cut(s) 945
BstZ17I GTATAC 1 cut(s) 668
Bsu15I ATCGAT 2 cut(s) 917, 1109
BsuI GTATCC 1 cut(s) 1133
BsuRI GGCC 1 cut(s) 1040
BsuTUI ATCGAT 2 cut(s) 917, 1109
BtsCI GGATG 2 cut(s) 797, 1026
BtsIMutI CAGTG 1 cut(s) 901
BtuMI TCGCGA 1 cut(s) 77
Cac8I GCNNGC 4 cut(s) 210, 733, 880, 1008
CfoI GCGC 2 cut(s) 151, 247
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 1 cut(s) 872
Cfr9I CCCGGG 1 cut(s) 949
ClaI ATCGAT 2 cut(s) 917, 1109
CseI GACGC 3 cut(s) 128, 155, 546
CsiI ACCWGGT 1 cut(s) 583
Csp6I GTAC 4 cut(s) 372, 516, 826, 954
CviAII CATG 3 cut(s) 326, 1003, 1020
CviJI RGCY 8 cut(s) 46, 82, 688, 712, 731, 848, 882, 1040
CviKI_1 RGCY 8 cut(s) 46, 82, 688, 712, 731, 848, 882, 1040
CviQI GTAC 4 cut(s) 372, 516, 826, 954
DdeI CTNAG 2 cut(s) 507, 591
DpnI GATC 8 cut(s) 341, 491, 652, 660, 916, 920, 947, 1112
DpnII GATC 8 cut(s) 339, 489, 650, 658, 914, 918, 945, 1110
DraIII CACNNNGTG 2 cut(s) 587, 896
DrdI GACNNNNNNGTC 1 cut(s) 409
DriI GACNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 409
Eam1104I CTCTTC 1 cut(s) 74
Eam1105I GACNNNNNGTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 74
EciI GGCGGA 4 cut(s) 19, 178, 534, 756
Eco47I GGWCC 1 cut(s) 872
Eco88I CYCGRG 1 cut(s) 949
EcoNI CCTNNNNNAGG 1 cut(s) 1034
EcoO109I RGGNCCY 1 cut(s) 872
EcoRI GAATTC 2 cut(s) 223, 562
EcoRII CCWGG 2 cut(s) 583, 616
FaeI CATG 3 cut(s) 329, 1006, 1023
FaqI GGGAC 1 cut(s) 872
FatI CATG 3 cut(s) 325, 1002, 1019
FauI CCCGC 2 cut(s) 59, 205
FblI GTMKAC 2 cut(s) 144, 667
FokI GGATG 2 cut(s) 784, 1013
FspBI CTAG 3 cut(s) 456, 519, 879
GlaI GCGC 2 cut(s) 150, 246
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 1 cut(s) 1040
HapII CCGG 5 cut(s) 83, 704, 767, 950, 1034
HgaI GACGC 3 cut(s) 128, 155, 546
HhaI GCGC 2 cut(s) 151, 247
Hin1I GRCGYC 2 cut(s) 255, 557
Hin1II CATG 3 cut(s) 329, 1006, 1023
Hin6I GCGC 2 cut(s) 149, 245
HinP1I GCGC 2 cut(s) 149, 245
HincII GTYRAC 2 cut(s) 145, 310
HindII GTYRAC 2 cut(s) 145, 310
HinfI GANTC 6 cut(s) 131, 185, 350, 427, 770, 841
HpaI GTTAAC 1 cut(s) 310
HpaII CCGG 5 cut(s) 83, 704, 767, 950, 1034
HphI GGTGA 4 cut(s) 645, 756, 1093, 1105
Hpy166II GTNNAC 4 cut(s) 145, 310, 354, 668
Hpy188III TCNNGA 4 cut(s) 76, 347, 833, 943
Hpy8I GTNNAC 4 cut(s) 145, 310, 354, 668
Hpy99I CGWCG 8 cut(s) 119, 122, 146, 149, 257, 415, 562, 677
HpyAV CCTTC 2 cut(s) 57, 771
HpyCH4III ACNGT 2 cut(s) 371, 484
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 9 cut(s) 231, 466, 709, 779, 797, 851, 1023, 1053, 1075
HpyF10VI GCNNNNNNNGC 5 cut(s) 536, 694, 709, 978, 981
HpyF3I CTNAG 2 cut(s) 507, 591
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 2 cut(s) 255, 557
Hsp92II CATG 3 cut(s) 329, 1006, 1023
HspAI GCGC 2 cut(s) 149, 245
KpnI GGTACC 1 cut(s) 957
KspAI GTTAAC 1 cut(s) 310
Kzo9I GATC 8 cut(s) 339, 489, 650, 658, 914, 918, 945, 1110
LmnI GCTCC 2 cut(s) 496, 547
Lsp1109I GCAGC 8 cut(s) 514, 526, 721, 835, 984, 987, 990, 993
LweI GCATC 3 cut(s) 453, 806, 889
MabI ACCWGGT 1 cut(s) 583
MaeI CTAG 3 cut(s) 456, 519, 879
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 1082
MalI GATC 8 cut(s) 341, 491, 652, 660, 916, 920, 947, 1112
MboI GATC 8 cut(s) 339, 489, 650, 658, 914, 918, 945, 1110
MboII GAAGA 6 cut(s) 20, 61, 64, 250, 384, 387
MflI RGATCY 1 cut(s) 945
MhlI GDGCHC 2 cut(s) 109, 592
MluCI AATT 3 cut(s) 223, 415, 562
MlyI GAGTC 2 cut(s) 140, 359
MmeI TCCRAC 3 cut(s) 162, 301, 655
MseI TTAA 2 cut(s) 309, 723
MspI CCGG 5 cut(s) 83, 704, 767, 950, 1034
MspR9I CCNGG 4 cut(s) 585, 618, 950, 951
MvaI CCWGG 2 cut(s) 585, 618
MvnI CGCG 2 cut(s) 77, 149
MwoI GCNNNNNNNGC 5 cut(s) 536, 694, 709, 978, 981
NciI CCSGG 2 cut(s) 950, 951
NdeII GATC 8 cut(s) 339, 489, 650, 658, 914, 918, 945, 1110
NheI GCTAGC 1 cut(s) 878
NlaIII CATG 3 cut(s) 329, 1006, 1023
NlaIV GGNNCC 3 cut(s) 176, 947, 955
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 1082
NruI TCGCGA 1 cut(s) 77
NspI RCATGY 1 cut(s) 1023
NspV TTCGAA 2 cut(s) 6, 436
PcsI WCGNNNNNNNCGW 4 cut(s) 12, 126, 167, 407
PfeI GAWTC 4 cut(s) 185, 427, 770, 841
PfoI TCCNGGA 1 cut(s) 616
PleI GAGTC 2 cut(s) 139, 358
PpsI GAGTC 2 cut(s) 139, 358
PpuMI RGGWCCY 1 cut(s) 872
Psp5II RGGWCCY 1 cut(s) 872
Psp6I CCWGG 2 cut(s) 583, 616
PspGI CCWGG 2 cut(s) 583, 616
PspN4I GGNNCC 3 cut(s) 176, 947, 955
PspPI GGNCC 1 cut(s) 872
PspPPI RGGWCCY 1 cut(s) 872
PsuI RGATCY 1 cut(s) 945
RruI TCGCGA 1 cut(s) 77
RsaI GTAC 4 cut(s) 373, 517, 827, 955
RsaNI GTAC 4 cut(s) 372, 516, 826, 954
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 2 cut(s) 309, 723
Sau3AI GATC 8 cut(s) 339, 489, 650, 658, 914, 918, 945, 1110
Sau96I GGNCC 1 cut(s) 872
ScaI AGTACT 2 cut(s) 373, 517
SchI GAGTC 2 cut(s) 140, 359
ScrFI CCNGG 4 cut(s) 585, 618, 950, 951
SduI GDGCHC 2 cut(s) 109, 592
SexAI ACCWGGT 1 cut(s) 583
SfaNI GCATC 3 cut(s) 453, 806, 889
SfuI TTCGAA 2 cut(s) 6, 436
SgrDI CGTCGACG 1 cut(s) 143
SinI GGWCC 1 cut(s) 872
SmaI CCCGGG 1 cut(s) 951
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 518
Sse9I AATT 3 cut(s) 223, 415, 562
SspMI CTAG 3 cut(s) 456, 519, 879
StyD4I CCNGG 4 cut(s) 583, 616, 948, 949
TaaI ACNGT 2 cut(s) 371, 484
TaiI ACGT 1 cut(s) 258
TasI AATT 3 cut(s) 223, 415, 562
TatI WGTACW 3 cut(s) 371, 515, 825
TauI GCSGC 5 cut(s) 95, 550, 580, 738, 741
TfiI GAWTC 4 cut(s) 185, 427, 770, 841
Tru1I TTAA 2 cut(s) 309, 723
Tru9I TTAA 2 cut(s) 309, 723
TscAI CASTG 1 cut(s) 901
TseFI GTSAC 1 cut(s) 1082
TseI GCWGC 8 cut(s) 527, 539, 709, 848, 972, 975, 978, 981
Tsp45I GTSAC 1 cut(s) 1082
TspDTI ATGAA 5 cut(s) 314, 323, 387, 614, 644
TspGWI ACGGA 1 cut(s) 455
TspMI CCCGGG 1 cut(s) 949
TspRI CASTG 1 cut(s) 901
VpaK11BI GGWCC 1 cut(s) 872
XagI CCTNNNNNAGG 1 cut(s) 1034
XapI RAATTY 3 cut(s) 223, 415, 562
XceI RCATGY 1 cut(s) 1023
XmaI CCCGGG 1 cut(s) 949
XmiI GTMKAC 2 cut(s) 144, 667
XspI CTAG 3 cut(s) 456, 519, 879
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 2 cut(s) 373, 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.