pycom14g20360

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010115
Physical Location & Seq
Forward (+)
21407641 .. 21412417
4777 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2535 bp
ATGCAACCCCAGAAAGGTGCTCGCTTGCAGATTCTGGAAAAAAAGATTAGCATACTGGAGAAGGCGACAGAGCTTAACCCAGATAATGAAGAACTGTTGCTTCCTCTTCTAAAGGCTTATCAAAGGAGGGACAACTCTGATGTATTAATTAGTAGATGGGAAAGGATACTTATACAACATTCAGGAAGTTACAGGTTGTGGAGAGAATTTTTGCGCGTCATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGACATGAGAAAAATGTACACACATGCAATCCAGGCTCTAGCTTCTGCTTGCAGAAAGCACATTAGGCAGGTTTGCCAAGCAGAAAACCATCCTCCAGATGCTGCTACAGTTCAACTAGAACTTGGTCTGGTTGATATATTTCTCAGTTTTTGTAGGTTTGAATGGCAGGCTGGCTATCAAGAGTTGGCCACTGCTCTATTTCAGGCTGAAATTGAATTCAGTTTGTTTTGTCCGTCTTTACTCCTCACCGAACAGAGTAAACAGATACTGTTCGAGCACTTTTGGAACAGTGATGGTGCAAGAGTTGGAGAAGAAGGTGCTCTTGGTTGGTCTACATGGTTGGAGAAAGAGGAAGAGAATAGGCAAAGGGTTATCAGAGAGGAGACTGCACATGATGATGAAGGTGGCTGGACAGGTTGGTCAGAACCACTCGCACAAAATAAGGAAAATAGTATAGAGACAGAAAAGGAGTCGGAAAGTAATGTAGTGGTTGAGGAGTTTCAAGAGGAACTTGAGGATGAAGATGTCAAGCAAGAAGAAGATACTGAAGCTTTGCTAAAGATGCTAGGGATTGATGTTGATGTTGGTGCTAGTGGTGAGATTAAAGACAGTTCAACCTGGATTAAATGGTCAGAAGAAGAGTTATCAAGAGATTGTGTTCAATGGATGCCTGTTCGTACAAGAGCTGCTGTCTCTCATAATGTTGGTACACCTGATGGAGAAGCAGATGATCACCTTTCGAGAGTCATAATGTATGAAGATGTCAACGAATACCTGTTTTCTTTGAGCTCATCTGAGGCCCGTTTATCTCTAGTGTTACAATTCGTTGATTTCTTCGGCGGGAAAAGCTCTCAGTGGATATCTACAAACAGCTCCACTTGGGCCGAGAAGGTCCTTAGCTTGGAGGCATTGCCAGATTCTATATTACAAACTTTGAGAAAGGTTCATGATTTTTTAATCAAAACACAAGGCAGTTCAAGTAATTTCAGCTTGGAATCTCTCTTGGGTACCACCAATGACATCTACAGAAGAACAGACATGATGAAATTTCTTCGTAATGCTACCTTGCTCTGTTTATCTGCTTTTCCACGTCATTTTGTCCTAGAAGACGCTGCTTTAGTTGCTGAAGAGCTATCTGCTTTGAATTTAAATCCTTCCAGCCGTTCAGTTACCCCATGCCGAGCTCTGGCAAAGTTCCTCTTAAAGAGTGACCGTCAGGATGTGTTGCTATGTGGAGTTTATGCAAGAAGGGAGGCTTTTCATGGGAATATTGATCATGCAAGAAGAGTATTTGACATGGCATTGTCATCTATTGAAGGGCTTCCTTTGGAATTACGGTCAAATGCTTCTCTTCTACATTTCTGGTATGCTGAGACGGAGCTTGCTAATGAGCATGGCAATGGGTGTGAATCATCACTACGTGCAATGCATATTTTATTTTGCTTAGGAAGTGGTTCTACGTATAGTCCGTATAAATCTCACCCATCAAATTTGCAATTACTGAGAGCACGCCAAGGTTTCAAAGAAAAAATAAGAACAGTACAAATGGCATTGATGCGAGGTGTTATAGATGATCAATCTGTTGCTCTCATATGTTCTGCTGCTTTATTTGAAGAGTTAACCTCTGGATGGGCTGCGGGTATTGAAGTTCTAGATCAGGCTTTTGCATTGGTGCTTCCAGAGAGAAAGAGCCGTAGTTATCAACTTGAATTTATGTTCAGCTTTTATATGAAGATGCTTTGGAGACATCATGGTCAGTCAAGTCTATCAAAATGCTGGGAGTCCATATTGCAGGGGCTTCAAATATATCCATGCAGCCCTGAACTATTTAGTGATTTCATCAAAGTTGGCCATCTTTATACGACACCTAATAAACTGCGGTGGGTGTTTGACGACCACTGTCACAAGAAACCTTCTGTGGTCGTCTGGCTTTTTGCATTGTCATTTGAATTGAGTAAAGGAGGATCGGAGCATAGGATCCGTGGATTATTTGAGAGGGCATTGGGAGATGATAGGTTTCATAACTCAGTTGTTCTTTGGCGATGCTACATTGCATATGAGATGAACATGGCAAAAAATCCTTCAGCAGCTAGGAGAAACTTCTTCCGGGCTATCCATGCCTGCCCGTGGTCAAAAAAGCTATGGCTTGATGGTTTTATGAAATTGAATTCCATCTTATCTGCAAAAGAGCTGTCGGATCTCCAAGAAGTCATGCGGGATAAGGAACTGAATCTGAGGACGGACATTTACGAAATTCTTTTGCAAGATGAGTTTGTATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

845

Amino Acids

96.83

Weight (kDa)

5.46

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 5 - 365 4.7e-69 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 670, 2004
Acc36I ACCTGC 1 cut(s) 310
Acc65I GGTACC 1 cut(s) 1259
AccB1I GGYRCC 1 cut(s) 1259
AccI GTMKAC 1 cut(s) 584
AccII CGCG 1 cut(s) 216
AciI CCGC 4 cut(s) 1092, 1889, 2131, 2467
AclWI GGATC 4 cut(s) 2224, 2224, 2237, 2457
AcoI YGGCCR 2 cut(s) 438, 2101
AcsI RAATTY 8 cut(s) 206, 467, 1298, 1396, 1741, 1961, 2419, 2505
AcuI CTGAAG 3 cut(s) 819, 1398, 2319
AdeI CACNNNGTG 1 cut(s) 1673
AfaI GTAC 5 cut(s) 269, 931, 961, 1261, 1794
AfiI CCNNNNNNNGG 5 cut(s) 14, 1153, 1438, 1881, 2379
AjiI CACGTC 1 cut(s) 1343
AjnI CCWGG 3 cut(s) 236, 282, 869
AjuI GAANNNNNNNTTGG 2 cut(s) 558, 590
Alw21I GWGCWC 6 cut(s) 22, 531, 574, 1043, 1438, 1762
Alw26I GTCTC 5 cut(s) 629, 704, 949, 1619, 1990
AlwI GGATC 4 cut(s) 2224, 2224, 2237, 2457
AlwNI CAGNNNCTG 3 cut(s) 34, 353, 520
AoxI GGCC 4 cut(s) 438, 1050, 1134, 2101
ApeKI GCWGC 7 cut(s) 353, 938, 1364, 1853, 1886, 2067, 2339
ApoI RAATTY 8 cut(s) 206, 467, 1298, 1396, 1741, 1961, 2419, 2505
ArsI GACNNNNNNTTYG 2 cut(s) 2429, 2461
AseI ATTAAT 1 cut(s) 146
Asp700I GAANNNNTTC 2 cut(s) 1572, 1705
Asp718I GGTACC 1 cut(s) 1259
AspLEI GCGC 1 cut(s) 216
AspS9I GGNCC 3 cut(s) 1051, 1134, 1144
AsuC2I CCSGG 1 cut(s) 2360
AsuHPI GGTGA 4 cut(s) 490, 860, 977, 1724
AvaII GGWCC 1 cut(s) 1144
BalI TGGCCA 2 cut(s) 440, 2103
BamHI GGATCC 1 cut(s) 2229
BanI GGYRCC 1 cut(s) 1259
BanII GRGCYC 2 cut(s) 1043, 1438
BarI GAAGNNNNNNTAC 2 cut(s) 1693, 1725
BbsI GAAGAC 1 cut(s) 1365
Bbv12I GWGCWC 6 cut(s) 22, 531, 574, 1043, 1438, 1762
BbvI GCAGC 7 cut(s) 340, 925, 1351, 1840, 1873, 2079, 2351
BccI CCATC 9 cut(s) 150, 348, 539, 962, 1744, 1875, 2112, 2396, 2432
BceAI ACGGC 2 cut(s) 1398, 1929
BciT130I CCWGG 3 cut(s) 238, 284, 871
BciVI GTATCC 1 cut(s) 159
BclI TGATCA 3 cut(s) 982, 1525, 1825
BcnI CCSGG 1 cut(s) 2360
BcoDI GTCTC 5 cut(s) 629, 704, 949, 1619, 1990
BfaI CTAG 8 cut(s) 290, 368, 818, 843, 1064, 1355, 1904, 2343
BfmI CTRYAG 2 cut(s) 357, 1276
BfuAI ACCTGC 1 cut(s) 310
BfuI GTATCC 1 cut(s) 159
BisI GCNGC 7 cut(s) 354, 939, 1365, 1854, 1887, 2068, 2340
BlsI GCNGC 7 cut(s) 355, 940, 1366, 1855, 1888, 2069, 2341
Bme1390I CCNGG 4 cut(s) 238, 284, 871, 2360
Bme18I GGWCC 1 cut(s) 1144
BmgBI CACGTC 1 cut(s) 1343
BmgT120I GGNCC 3 cut(s) 1051, 1134, 1144
BmiI GGNNCC 2 cut(s) 1261, 2231
BmrFI CCNGG 4 cut(s) 238, 284, 871, 2360
BmsI GCATC 6 cut(s) 340, 804, 909, 1797, 1977, 2285
BoxI GACNNNNGTC 1 cut(s) 2151
BpiI GAAGAC 1 cut(s) 1365
BplI GAGNNNNNCTC 2 cut(s) 1859, 1891
BpmI CTGGAG 3 cut(s) 77, 220, 330
Bpu10I CCTNAGC 2 cut(s) 1148, 1696
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 2360
BsaAI YACGTR 2 cut(s) 1673, 1713
BsaJI CCNNGG 3 cut(s) 1765, 2233, 2378
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 1153, 1438, 1881, 2379
Bse1I ACTGG 1 cut(s) 60
Bse3DI GCAATG 4 cut(s) 1160, 1657, 1683, 2301
BseBI CCWGG 3 cut(s) 238, 284, 871
BseDI CCNNGG 3 cut(s) 1765, 2233, 2378
BseGI GGATG 5 cut(s) 340, 775, 924, 1477, 1886
BseLI CCNNNNNNNGG 5 cut(s) 14, 1153, 1438, 1881, 2379
BseMI GCAATG 4 cut(s) 1160, 1657, 1683, 2301
BseMII CTCAG 7 cut(s) 409, 1038, 1118, 1614, 1745, 2292, 2477
BseNI ACTGG 1 cut(s) 60
BseRI GAGGAG 3 cut(s) 485, 647, 761
BseXI GCAGC 7 cut(s) 340, 925, 1351, 1840, 1873, 2079, 2351
BseYI CCCAGC 1 cut(s) 2028
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 440, 1052, 1136, 2103
BshNI GGYRCC 1 cut(s) 1259
BsiHKAI GWGCWC 6 cut(s) 22, 531, 574, 1043, 1438, 1762
BsiSI CCGG 1 cut(s) 2359
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 5 cut(s) 14, 1153, 1438, 1881, 2379
BsmAI GTCTC 5 cut(s) 629, 704, 949, 1619, 1990
BsmBI CGTCTC 1 cut(s) 1619
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 4 cut(s) 440, 1052, 1136, 2103
Bsp1286I GDGCHC 6 cut(s) 22, 531, 574, 1043, 1438, 1762
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 7 cut(s) 982, 1525, 1825, 1906, 2216, 2229, 2449
BspACI CCGC 4 cut(s) 1092, 1889, 2131, 2467
BspANI GGCC 4 cut(s) 440, 1052, 1136, 2103
BspCNI CTCAG 7 cut(s) 408, 1039, 1117, 1615, 1746, 2291, 2478
BspFNI CGCG 1 cut(s) 216
BspHI TCATGA 1 cut(s) 1198
BspLI GGNNCC 2 cut(s) 1261, 2231
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 4 cut(s) 2224, 2224, 2237, 2457
BspQI GCTCTTC 1 cut(s) 1374
BspT107I GGYRCC 1 cut(s) 1259
BsrDI GCAATG 4 cut(s) 1160, 1657, 1683, 2301
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 3 cut(s) 1765, 2233, 2378
BssMI GATC 7 cut(s) 982, 1525, 1825, 1906, 2216, 2229, 2449
BssT1I CCWWGG 1 cut(s) 1765
Bst2UI CCWGG 3 cut(s) 238, 284, 871
Bst4CI ACNGT 9 cut(s) 96, 361, 522, 542, 863, 1466, 1590, 1792, 2153
Bst6I CTCTTC 7 cut(s) 111, 600, 885, 1374, 1531, 1608, 1860
BstAUI TGTACA 1 cut(s) 267
BstBAI YACGTR 2 cut(s) 1673, 1713
BstC8I GCNNGC 8 cut(s) 22, 26, 301, 420, 424, 1635, 1762, 2374
BstDEI CTNAG 9 cut(s) 395, 1047, 1104, 1148, 1623, 1696, 1754, 2278, 2486
BstDSI CCRYGG 2 cut(s) 2233, 2378
BstF5I GGATG 5 cut(s) 340, 775, 924, 1477, 1886
BstFNI CGCG 1 cut(s) 216
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 7 cut(s) 985, 1528, 1828, 1909, 2219, 2232, 2452
BstMAI GTCTC 5 cut(s) 629, 704, 949, 1619, 1990
BstMBI GATC 7 cut(s) 982, 1525, 1825, 1906, 2216, 2229, 2449
BstMWI GCNNNNNNNGC 6 cut(s) 284, 316, 814, 1098, 1373, 2369
BstNI CCWGG 3 cut(s) 238, 284, 871
BstNSI RCATGY 1 cut(s) 278
BstPAI GACNNNNGTC 1 cut(s) 2151
BstSCI CCNGG 4 cut(s) 236, 282, 869, 2358
BstSFI CTRYAG 2 cut(s) 357, 1276
BstSNI TACGTA 1 cut(s) 1713
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 7 cut(s) 340, 925, 1351, 1840, 1873, 2079, 2351
BstV2I GAAGAC 1 cut(s) 1365
BstX2I RGATCY 2 cut(s) 2229, 2449
BstYI RGATCY 2 cut(s) 2229, 2449
BsuI GTATCC 1 cut(s) 159
BsuRI GGCC 4 cut(s) 440, 1052, 1136, 2103
BtgI CCRYGG 2 cut(s) 2233, 2378
BtgZI GCGATG 1 cut(s) 2308
BtrI CACGTC 1 cut(s) 1343
BtsCI GGATG 5 cut(s) 340, 775, 924, 1477, 1886
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 4 cut(s) 441, 547, 1112, 2149
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 8 cut(s) 22, 26, 301, 420, 424, 1635, 1762, 2374
CaiI CAGNNNCTG 3 cut(s) 34, 353, 520
CciI TCATGA 1 cut(s) 1198
CfoI GCGC 1 cut(s) 216
Cfr13I GGNCC 3 cut(s) 1051, 1134, 1144
CseI GACGC 2 cut(s) 205, 1370
Csp6I GTAC 5 cut(s) 268, 930, 960, 1260, 1793
CviQI GTAC 5 cut(s) 268, 930, 960, 1260, 1793
DdeI CTNAG 9 cut(s) 395, 1047, 1104, 1148, 1623, 1696, 1754, 2278, 2486
DpnI GATC 7 cut(s) 984, 1527, 1827, 1908, 2218, 2231, 2451
DpnII GATC 7 cut(s) 982, 1525, 1825, 1906, 2216, 2229, 2449
DraI TTTAAA 1 cut(s) 1401
DraIII CACNNNGTG 1 cut(s) 1673
DrdI GACNNNNNNGTC 2 cut(s) 670, 2004
DseDI GACNNNNNNGTC 2 cut(s) 670, 2004
EaeI YGGCCR 2 cut(s) 438, 2101
Eam1104I CTCTTC 7 cut(s) 111, 600, 885, 1374, 1531, 1608, 1860
EarI CTCTTC 7 cut(s) 111, 600, 885, 1374, 1531, 1608, 1860
Ecl136II GAGCTC 2 cut(s) 1041, 1436
Eco105I TACGTA 1 cut(s) 1713
Eco130I CCWWGG 1 cut(s) 1765
Eco24I GRGCYC 2 cut(s) 1043, 1438
Eco32I GATATC 1 cut(s) 1113
Eco47I GGWCC 1 cut(s) 1144
Eco53kI GAGCTC 2 cut(s) 1041, 1436
Eco57I CTGAAG 3 cut(s) 819, 1398, 2319
EcoICRI GAGCTC 2 cut(s) 1041, 1436
EcoO109I RGGNCCY 1 cut(s) 1144
EcoRI GAATTC 2 cut(s) 467, 2419
EcoRII CCWGG 3 cut(s) 236, 282, 869
EcoRV GATATC 1 cut(s) 1113
EcoT14I CCWWGG 1 cut(s) 1765
EcoT22I ATGCAT 1 cut(s) 1683
EcoT38I GRGCYC 2 cut(s) 1043, 1438
ErhI CCWWGG 1 cut(s) 1765
Esp3I CGTCTC 1 cut(s) 1619
FalI AAGNNNNNCTT 8 cut(s) 558, 590, 747, 779, 1436, 1468, 1492, 1524
FaqI GGGAC 1 cut(s) 143
FauI CCCGC 3 cut(s) 1085, 1882, 2460
FauNDI CATATG 2 cut(s) 1844, 2308
FbaI TGATCA 3 cut(s) 982, 1525, 1825
FblI GTMKAC 1 cut(s) 584
Fnu4HI GCNGC 7 cut(s) 354, 939, 1365, 1854, 1887, 2068, 2340
FokI GGATG 5 cut(s) 327, 782, 931, 1484, 1893
FriOI GRGCYC 2 cut(s) 1043, 1438
Fsp4HI GCNGC 7 cut(s) 354, 939, 1365, 1854, 1887, 2068, 2340
FspBI CTAG 8 cut(s) 290, 368, 818, 843, 1064, 1355, 1904, 2343
GlaI GCGC 1 cut(s) 215
GluI GCNGC 7 cut(s) 354, 939, 1365, 1854, 1887, 2068, 2340
GsaI CCCAGC 1 cut(s) 2032
GsuI CTGGAG 3 cut(s) 77, 220, 330
HaeIII GGCC 4 cut(s) 440, 1052, 1136, 2103
HapII CCGG 1 cut(s) 2359
HgaI GACGC 2 cut(s) 205, 1370
HhaI GCGC 1 cut(s) 216
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HincII GTYRAC 2 cut(s) 1018, 1872
HindII GTYRAC 2 cut(s) 1018, 1872
HindIII AAGCTT 1 cut(s) 801
HinfI GANTC 8 cut(s) 31, 722, 996, 1169, 1247, 1661, 2033, 2482
HpaI GTTAAC 1 cut(s) 1872
HpaII CCGG 1 cut(s) 2359
HphI GGTGA 4 cut(s) 490, 860, 977, 1724
Hpy166II GTNNAC 6 cut(s) 270, 512, 585, 962, 1018, 1872
Hpy8I GTNNAC 6 cut(s) 270, 512, 585, 962, 1018, 1872
HpyAV CCTTC 9 cut(s) 55, 560, 647, 1135, 1416, 1494, 1562, 2175, 2343
HpyCH4III ACNGT 9 cut(s) 96, 361, 522, 542, 863, 1466, 1590, 1792, 2153
HpyCH4IV ACGT 3 cut(s) 1342, 1672, 1712
HpyF10VI GCNNNNNNNGC 6 cut(s) 284, 316, 814, 1098, 1373, 2369
HpyF3I CTNAG 9 cut(s) 395, 1047, 1104, 1148, 1623, 1696, 1754, 2278, 2486
HpySE526I ACGT 3 cut(s) 1342, 1672, 1712
HspAI GCGC 1 cut(s) 214
KpnI GGTACC 1 cut(s) 1263
Ksp22I TGATCA 3 cut(s) 982, 1525, 1825
KspAI GTTAAC 1 cut(s) 1872
Kzo9I GATC 7 cut(s) 982, 1525, 1825, 1906, 2216, 2229, 2449
LguI GCTCTTC 1 cut(s) 1374
LmnI GCTCC 3 cut(s) 1130, 1630, 2221
Lsp1109I GCAGC 7 cut(s) 340, 925, 1351, 1840, 1873, 2079, 2351
LweI GCATC 6 cut(s) 340, 804, 909, 1797, 1977, 2285
MaeI CTAG 8 cut(s) 290, 368, 818, 843, 1064, 1355, 1904, 2343
MaeII ACGT 3 cut(s) 1342, 1672, 1712
MaeIII GTNAC 5 cut(s) 188, 1068, 1420, 1460, 2153
MalI GATC 7 cut(s) 984, 1527, 1827, 1908, 2218, 2231, 2451
MboI GATC 7 cut(s) 982, 1525, 1825, 1906, 2216, 2229, 2449
MflI RGATCY 2 cut(s) 2229, 2449
MhlI GDGCHC 6 cut(s) 22, 531, 574, 1043, 1438, 1762
MlsI TGGCCA 2 cut(s) 440, 2103
MluNI TGGCCA 2 cut(s) 440, 2103
MlyI GAGTC 3 cut(s) 731, 1005, 2042
MmeI TCCRAC 4 cut(s) 538, 573, 705, 2427
Mox20I TGGCCA 2 cut(s) 440, 2103
Mph1103I ATGCAT 1 cut(s) 1683
MroXI GAANNNNTTC 2 cut(s) 1572, 1705
MscI TGGCCA 2 cut(s) 440, 2103
MseI TTAA 8 cut(s) 75, 146, 855, 876, 1208, 1400, 1454, 1871
MslI CAYNNNNRTG 2 cut(s) 648, 1650
Msp20I TGGCCA 2 cut(s) 440, 2103
MspI CCGG 1 cut(s) 2359
MspR9I CCNGG 4 cut(s) 238, 284, 871, 2360
MvaI CCWGG 3 cut(s) 238, 284, 871
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 6 cut(s) 284, 316, 814, 1098, 1373, 2369
NciI CCSGG 1 cut(s) 2360
NdeI CATATG 2 cut(s) 1844, 2308
NdeII GATC 7 cut(s) 982, 1525, 1825, 1906, 2216, 2229, 2449
NlaIV GGNNCC 2 cut(s) 1261, 2231
NmeAIII GCCGAG 2 cut(s) 1162, 1457
NmuCI GTSAC 2 cut(s) 1460, 2153
NsiI ATGCAT 1 cut(s) 1683
NspI RCATGY 1 cut(s) 278
PagI TCATGA 1 cut(s) 1198
PciSI GCTCTTC 1 cut(s) 1374
PcsI WCGNNNNNNNCGW 1 cut(s) 1718
PdmI GAANNNNTTC 2 cut(s) 1572, 1705
PfeI GAWTC 5 cut(s) 31, 1169, 1247, 1661, 2482
PkrI GCNGC 7 cut(s) 355, 940, 1366, 1855, 1888, 2069, 2341
PleI GAGTC 3 cut(s) 730, 1004, 2041
PpsI GAGTC 3 cut(s) 730, 1004, 2041
Ppu21I YACGTR 2 cut(s) 1673, 1713
PpuMI RGGWCCY 1 cut(s) 1144
PshAI GACNNNNGTC 1 cut(s) 2151
PshBI ATTAAT 1 cut(s) 146
Psp124BI GAGCTC 2 cut(s) 1043, 1438
Psp5II RGGWCCY 1 cut(s) 1144
Psp6I CCWGG 3 cut(s) 236, 282, 869
PspFI CCCAGC 1 cut(s) 2028
PspGI CCWGG 3 cut(s) 236, 282, 869
PspN4I GGNNCC 2 cut(s) 1261, 2231
PspPI GGNCC 3 cut(s) 1051, 1134, 1144
PspPPI RGGWCCY 1 cut(s) 1144
PstNI CAGNNNCTG 3 cut(s) 34, 353, 520
PsuI RGATCY 2 cut(s) 2229, 2449
RsaI GTAC 5 cut(s) 269, 931, 961, 1261, 1794
RsaNI GTAC 5 cut(s) 268, 930, 960, 1260, 1793
RseI CAYNNNNRTG 2 cut(s) 648, 1650
SacI GAGCTC 2 cut(s) 1043, 1438
SapI GCTCTTC 1 cut(s) 1374
SaqAI TTAA 8 cut(s) 75, 146, 855, 876, 1208, 1400, 1454, 1871
SatI GCNGC 7 cut(s) 354, 939, 1365, 1854, 1887, 2068, 2340
Sau3AI GATC 7 cut(s) 982, 1525, 1825, 1906, 2216, 2229, 2449
Sau96I GGNCC 3 cut(s) 1051, 1134, 1144
SchI GAGTC 3 cut(s) 731, 1005, 2042
ScrFI CCNGG 4 cut(s) 238, 284, 871, 2360
SduI GDGCHC 6 cut(s) 22, 531, 574, 1043, 1438, 1762
SfaNI GCATC 6 cut(s) 340, 804, 909, 1797, 1977, 2285
SfcI CTRYAG 2 cut(s) 357, 1276
SinI GGWCC 1 cut(s) 1144
SmiI ATTTAAAT 1 cut(s) 1401
SmiMI CAYNNNNRTG 2 cut(s) 648, 1650
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
SnaBI TACGTA 1 cut(s) 1713
SsiI CCGC 4 cut(s) 1092, 1889, 2131, 2467
SspI AATATT 1 cut(s) 1522
SspMI CTAG 8 cut(s) 290, 368, 818, 843, 1064, 1355, 1904, 2343
SstI GAGCTC 2 cut(s) 1043, 1438
StyD4I CCNGG 4 cut(s) 236, 282, 869, 2358
StyI CCWWGG 1 cut(s) 1765
SwaI ATTTAAAT 1 cut(s) 1401
TaaI ACNGT 9 cut(s) 96, 361, 522, 542, 863, 1466, 1590, 1792, 2153
TaiI ACGT 3 cut(s) 1345, 1675, 1715
TaqI TCGA 2 cut(s) 525, 992
TatI WGTACW 2 cut(s) 267, 1792
TfiI GAWTC 5 cut(s) 31, 1169, 1247, 1661, 2482
Tru1I TTAA 8 cut(s) 75, 146, 855, 876, 1208, 1400, 1454, 1871
Tru9I TTAA 8 cut(s) 75, 146, 855, 876, 1208, 1400, 1454, 1871
TscAI CASTG 4 cut(s) 448, 547, 1112, 2156
TseFI GTSAC 2 cut(s) 1460, 2153
TseI GCWGC 7 cut(s) 353, 938, 1364, 1853, 1886, 2067, 2339
Tsp45I GTSAC 2 cut(s) 1460, 2153
TspGWI ACGGA 5 cut(s) 474, 1643, 1710, 2222, 2507
TspRI CASTG 4 cut(s) 448, 547, 1112, 2156
VpaK11BI GGWCC 1 cut(s) 1144
VspI ATTAAT 1 cut(s) 146
XapI RAATTY 8 cut(s) 206, 467, 1298, 1396, 1741, 1961, 2419, 2505
XbaI TCTAGA 1 cut(s) 1903
XceI RCATGY 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 584
XmnI GAANNNNTTC 2 cut(s) 1572, 1705
XspI CTAG 8 cut(s) 290, 368, 818, 843, 1064, 1355, 1904, 2343
Zsp2I ATGCAT 1 cut(s) 1683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.