pycom15g00990

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011405
Physical Location & Seq
Reverse (-)
530461 .. 532152
1692 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ATGTCAGTGTGGTATGGGCTTATAGCAAATAACCTGAGAGGGGAAAGAGAGGCGATGGCTGCTCACGCAGGACGGCTTCTAAGGCAATGGCCGATGTGGCAGCAGCATTGCTGCTCAAACGCCGCCTTCCACCACCTTCTCTACTCCCCCCTACTTCCAGTCACCACCAGAACCAACTCCCGTTGTCCATCTTTGACCACCATCCACCTGTCCACTCGCGCCGTCCATAAACTATTTCGCGGGCTCAGACGGTCCGACTACCAAGATGCCAGCAACACCGGCAGTTCGGGTTCGGGTTCGGATTCGGATGCAGTCACGTACAAGAGCCGAAACGAGTTGAAGCGCCACGCCAGACGCGCCGTCCGTTGGGCTATGGACCTCGCCTCCTTCTCCACCCCTCAAATCAAACGCATTGTCAGAGTAGCTTCTCTGGACCAGGACGTGTTGGATGCTGTGATTCTAGTGAAGAAACTAGGACCGGATGTTCGAGAAGGGAAGCGAAGGCAGTTCAATTATATTGGGAAACTGCTGCGGGATGTGGAGGCTGACTTGATGGATGCTTTGATTCAGGCTACGAAAGACAGTGACGAAAGTAAGCTACAAGCTTTGTCAGGTCCGGAGACATTGGGAGTTGATGATGTTGCCGATGCAGAAGAAGCAGAAGAAACTGATTATGAGGAGGAAGAAGAGGGTTCTCACATTGATGAAGCAACCAGATGGTTTGATGGTCTGGTCAGTAAGGACATTAAGGTCACCAATGAAGTATATTCAATTTCAAGTGTTGAGTTTGACCGTCAGGAACTAAGGAAACTTGTCCGAAGTGTGCATTCTAGATTGGAACAGAAGGTTGATTCGGAGGAAAATACGGTAAAGATTGATGCAGCAAGAGTGAGAGCTGAAAAGGCACTCACCAGGTTCCTTCGTTGCCTTGCTAAACGTAGCCTAGAGATTGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

320

Amino Acids

36.15

Weight (kDa)

6.53

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 107 - 308 1.2e-27 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 749
AccII CGCG 3 cut(s) 219, 240, 357
AccIII TCCGGA 1 cut(s) 616
AciI CCGC 3 cut(s) 123, 240, 532
AcoI YGGCCR 1 cut(s) 89
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 40, 366
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 4 cut(s) 340, 511, 771, 777
AhdI GACNNNNNGTC 1 cut(s) 359
AjiI CACGTC 1 cut(s) 442
AjnI CCWGG 2 cut(s) 435, 911
AloI GAACNNNNNNTCC 2 cut(s) 468, 500
AluBI AGCT 4 cut(s) 425, 598, 605, 896
AluI AGCT 4 cut(s) 425, 598, 605, 896
Alw26I GTCTC 1 cut(s) 614
Aor13HI TCCGGA 1 cut(s) 616
AoxI GGCC 1 cut(s) 89
ApeKI GCWGC 6 cut(s) 59, 100, 103, 111, 529, 881
ArsI GACNNNNNNTTYG 4 cut(s) 399, 431, 468, 500
AspLEI GCGC 3 cut(s) 221, 345, 359
AspS9I GGNCC 5 cut(s) 252, 376, 433, 476, 614
AsuHPI GGTGA 3 cut(s) 154, 745, 901
AvaII GGWCC 5 cut(s) 252, 376, 433, 476, 614
BanII GRGCYC 1 cut(s) 246
BbvI GCAGC 6 cut(s) 46, 98, 112, 115, 516, 893
BccI CCATC 6 cut(s) 49, 196, 209, 547, 711, 719
BceAI ACGGC 3 cut(s) 89, 206, 344
BciT130I CCWGG 2 cut(s) 437, 913
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 4 cut(s) 461, 473, 831, 944
BfoI RGCGCY 1 cut(s) 346
BglI GCCNNNNNGGC 1 cut(s) 97
BisI GCNGC 7 cut(s) 60, 101, 104, 112, 123, 530, 882
BlsI GCNGC 7 cut(s) 61, 102, 105, 113, 124, 531, 883
Bme1390I CCNGG 2 cut(s) 437, 913
Bme18I GGWCC 5 cut(s) 252, 376, 433, 476, 614
BmeRI GACNNNNNGTC 1 cut(s) 359
BmgBI CACGTC 1 cut(s) 442
BmgT120I GGNCC 5 cut(s) 252, 376, 433, 476, 614
BmiI GGNNCC 1 cut(s) 917
BmrFI CCNGG 2 cut(s) 437, 913
BmsI GCATC 6 cut(s) 256, 298, 439, 547, 637, 868
BsaAI YACGTR 1 cut(s) 318
BsaWI WCCGGW 2 cut(s) 478, 616
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 366
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 1 cut(s) 158
Bse3DI GCAATG 2 cut(s) 92, 106
BseAI TCCGGA 1 cut(s) 616
BseBI CCWGG 2 cut(s) 437, 913
BseGI GGATG 6 cut(s) 201, 313, 454, 487, 541, 562
BseLI CCNNNNNNNGG 2 cut(s) 40, 366
BseMI GCAATG 2 cut(s) 92, 106
BseMII CTCAG 2 cut(s) 26, 259
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 1 cut(s) 692
BseXI GCAGC 6 cut(s) 46, 98, 112, 115, 516, 893
Bsh1236I CGCG 3 cut(s) 219, 240, 357
BshFI GGCC 1 cut(s) 91
BsiSI CCGG 3 cut(s) 279, 479, 617
BslI CCNNNNNNNGG 2 cut(s) 40, 366
BsmAI GTCTC 1 cut(s) 614
BsmI GAATGC 1 cut(s) 826
BsnI GGCC 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 246
Bsp13I TCCGGA 1 cut(s) 616
BspACI CCGC 3 cut(s) 123, 240, 532
BspANI GGCC 1 cut(s) 91
BspCNI CTCAG 2 cut(s) 27, 258
BspEI TCCGGA 1 cut(s) 616
BspFNI CGCG 3 cut(s) 219, 240, 357
BspLI GGNNCC 1 cut(s) 917
BsrDI GCAATG 2 cut(s) 92, 106
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 437, 913
Bst4CI ACNGT 4 cut(s) 252, 584, 794, 868
Bst6I CTCTTC 1 cut(s) 681
BstBAI YACGTR 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 242, 271
BstDEI CTNAG 4 cut(s) 35, 80, 245, 803
BstEII GGTNACC 1 cut(s) 751
BstF5I GGATG 6 cut(s) 201, 313, 454, 487, 541, 562
BstFNI CGCG 3 cut(s) 219, 240, 357
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 3 cut(s) 221, 345, 359
BstMAI GTCTC 1 cut(s) 614
BstMWI GCNNNNNNNGC 8 cut(s) 59, 65, 82, 97, 279, 356, 656, 902
BstNI CCWGG 2 cut(s) 437, 913
BstPI GGTNACC 1 cut(s) 751
BstSCI CCNGG 2 cut(s) 435, 911
BstUI CGCG 3 cut(s) 219, 240, 357
BstV1I GCAGC 6 cut(s) 46, 98, 112, 115, 516, 893
BsuRI GGCC 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 68
BtrI CACGTC 1 cut(s) 442
BtsCI GGATG 6 cut(s) 201, 313, 454, 487, 541, 562
BtsIMutI CAGTG 2 cut(s) 12, 589
Cac8I GCNNGC 2 cut(s) 242, 271
CfoI GCGC 3 cut(s) 221, 345, 359
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 5 cut(s) 252, 376, 433, 476, 614
CpoI CGGWCCG 1 cut(s) 252
CseI GACGC 1 cut(s) 363
CsiI ACCWGGT 1 cut(s) 911
Csp6I GTAC 1 cut(s) 319
CspI CGGWCCG 1 cut(s) 252
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 4 cut(s) 35, 80, 245, 803
DrdI GACNNNNNNGTC 1 cut(s) 749
DriI GACNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 749
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 1 cut(s) 681
Eam1105I GACNNNNNGTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 681
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 5 cut(s) 252, 376, 433, 476, 614
Eco91I GGTNACC 1 cut(s) 751
EcoO65I GGTNACC 1 cut(s) 751
EcoRII CCWGG 2 cut(s) 435, 911
EcoT38I GRGCYC 1 cut(s) 246
FaiI YATR 9 cut(s) 15, 23, 228, 374, 516, 675, 766, 956, 958
FauI CCCGC 2 cut(s) 233, 525
Fnu4HI GCNGC 7 cut(s) 60, 101, 104, 112, 123, 530, 882
FokI GGATG 6 cut(s) 188, 320, 461, 494, 548, 569
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 7 cut(s) 60, 101, 104, 112, 123, 530, 882
FspBI CTAG 4 cut(s) 461, 473, 831, 944
GlaI GCGC 3 cut(s) 220, 344, 358
GluI GCNGC 7 cut(s) 60, 101, 104, 112, 123, 530, 882
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 1 cut(s) 91
HapII CCGG 3 cut(s) 279, 479, 617
HgaI GACGC 1 cut(s) 363
HhaI GCGC 3 cut(s) 221, 345, 359
Hin6I GCGC 3 cut(s) 219, 343, 357
HinP1I GCGC 3 cut(s) 219, 343, 357
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 4 cut(s) 302, 457, 565, 851
HpaII CCGG 3 cut(s) 279, 479, 617
HphI GGTGA 3 cut(s) 154, 745, 901
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 7 cut(s) 248, 256, 301, 307, 419, 818, 856
Hpy188III TCNNGA 5 cut(s) 431, 488, 617, 797, 831
Hpy8I GTNNAC 1 cut(s) 213
HpyAV CCTTC 7 cut(s) 136, 146, 397, 485, 495, 838, 929
HpyCH4III ACNGT 4 cut(s) 252, 584, 794, 868
HpyCH4IV ACGT 3 cut(s) 317, 441, 937
HpyCH4V TGCA 4 cut(s) 311, 650, 826, 881
HpyF10VI GCNNNNNNNGC 8 cut(s) 59, 65, 82, 97, 279, 356, 656, 902
HpyF3I CTNAG 4 cut(s) 35, 80, 245, 803
HpySE526I ACGT 3 cut(s) 317, 441, 937
HspAI GCGC 3 cut(s) 219, 343, 357
Kpn2I TCCGGA 1 cut(s) 616
Lsp1109I GCAGC 6 cut(s) 46, 98, 112, 115, 516, 893
LweI GCATC 6 cut(s) 256, 298, 439, 547, 637, 868
MabI ACCWGGT 1 cut(s) 911
MaeI CTAG 4 cut(s) 461, 473, 831, 944
MaeII ACGT 3 cut(s) 317, 441, 937
MaeIII GTNAC 4 cut(s) 160, 313, 584, 751
MboII GAAGA 5 cut(s) 478, 665, 674, 695, 698
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 2 cut(s) 511, 771
MmeI TCCRAC 2 cut(s) 279, 426
MroI TCCGGA 1 cut(s) 616
MseI TTAA 1 cut(s) 747
MslI CAYNNNNRTG 1 cut(s) 702
MspI CCGG 3 cut(s) 279, 479, 617
MspR9I CCNGG 2 cut(s) 437, 913
Mva1269I GAATGC 1 cut(s) 826
MvaI CCWGG 2 cut(s) 437, 913
MvnI CGCG 3 cut(s) 219, 240, 357
MwoI GCNNNNNNNGC 8 cut(s) 59, 65, 82, 97, 279, 356, 656, 902
NlaIV GGNNCC 1 cut(s) 917
NmuCI GTSAC 4 cut(s) 160, 313, 584, 751
PcsI WCGNNNNNNNCGW 1 cut(s) 361
PctI GAATGC 1 cut(s) 826
PfeI GAWTC 4 cut(s) 302, 457, 565, 851
PkrI GCNGC 7 cut(s) 61, 102, 105, 113, 124, 531, 883
Ppu21I YACGTR 1 cut(s) 318
Psp6I CCWGG 2 cut(s) 435, 911
PspEI GGTNACC 1 cut(s) 751
PspGI CCWGG 2 cut(s) 435, 911
PspN4I GGNNCC 1 cut(s) 917
PspPI GGNCC 5 cut(s) 252, 376, 433, 476, 614
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 702
Rsr2I CGGWCCG 1 cut(s) 252
RsrII CGGWCCG 1 cut(s) 252
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 7 cut(s) 60, 101, 104, 112, 123, 530, 882
Sau96I GGNCC 5 cut(s) 252, 376, 433, 476, 614
ScrFI CCNGG 2 cut(s) 437, 913
SduI GDGCHC 1 cut(s) 246
SexAI ACCWGGT 1 cut(s) 911
SfaNI GCATC 6 cut(s) 256, 298, 439, 547, 637, 868
SinI GGWCC 5 cut(s) 252, 376, 433, 476, 614
SmiMI CAYNNNNRTG 1 cut(s) 702
Sse9I AATT 2 cut(s) 511, 771
SsiI CCGC 3 cut(s) 123, 240, 532
SspMI CTAG 4 cut(s) 461, 473, 831, 944
StyD4I CCNGG 2 cut(s) 435, 911
TaaI ACNGT 4 cut(s) 252, 584, 794, 868
TaiI ACGT 3 cut(s) 320, 444, 940
TaqI TCGA 1 cut(s) 487
TasI AATT 2 cut(s) 511, 771
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 4 cut(s) 302, 457, 565, 851
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TscAI CASTG 2 cut(s) 12, 589
TseFI GTSAC 4 cut(s) 160, 313, 584, 751
TseI GCWGC 6 cut(s) 59, 100, 103, 111, 529, 881
Tsp45I GTSAC 4 cut(s) 160, 313, 584, 751
TspDTI ATGAA 2 cut(s) 720, 774
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 2 cut(s) 12, 589
VpaK11BI GGWCC 5 cut(s) 252, 376, 433, 476, 614
XbaI TCTAGA 1 cut(s) 830
XspI CTAG 4 cut(s) 461, 473, 831, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.