Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0000943

Physical Location & Seq

Genomic Coordinates Reverse (-)

815026 .. 818705

Gene Structure 3680 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1338 bp

ATGATTTGTCGTTCATACTACATAAAATTCCAACTTCTGGTGCGTTCCTCTTCTTCCTTCGGTAGAACAGAGCACGACTGGCGCTGCCCAACCCAAGTTTACAGGGTCCTCGTTTTCACTCCTCAGCAGTCAGCTTCTCCACCGAGCGCCGGACTTGGGAAAGCAGTTCCGATGAGCAGCAAGCAGAGACGGCCGCCGGACCCAGTGGCGGTTCTGAGGGGCCACCGCGCCTCCGTCATGGACCTCTCTTTTCATCCTTCCCAACCACTCCTGTTCACCGGTTCATCGGATGGCGAGCTGAGGATTTGGGATACTATCCAGCATCGGACTGTATCATCAGCTGGGGTGCATAATGCAGCACATGGTATTGCTTCTGTTGCTTGTACTGCCAACAGAGTTATCAGCCAGGGCAGGGATGGGACGGTTAAATGTTGGGATATTGAAGGTGGAGGTCTGCCCAGGACTCCGTCTGTTACAATCAAGACAAACTCTTACCACTTTTGTAAACTTTCCTTGGTGAAGAGGCCTCAATCTTGTTCTACACGAGTGGACGGGGTTAGGTTTCATGATGGAGAGGACATAAGGGTTAGGGGGACCACTGATGAAGATACTCTAGATGATAGTAGAGACAACGTTGAAGGGAATCTGCCTGAGCATCCCAGCACCTTTGAAGAAAATACTCAAGATGAATTATCTAGATATGTTGCTGTAGCAGGGGAGCAGACTTCTGAGGTTGAGATTTGGGATCTAAATACTGCAGAAAGGTTTGCACGGCTTCCTCAAAGCTGCTTTGGTGGTTCCCCGGGTATTTCTTCCAAGGAGAGAGGAATGTGCATGGCTATTCAAGCATTCTCACCATCTGAATCACAGGGATTCCTAAATGTCTTGGTTGGTTATGAGGATGGTTCCATGCTTTGGTGGGATATTAGAAATCCGGGGGTTCCAATGACCTCTGTCAGGTTTCATTCAGAACCAGTTCTAAGTCTATGTGTGGATGGGTCATGCAATGGTGGTATCTCAGGAGCTGCAGATGAGAAAGTTGTGCTGTATAGTTTGGATCCTTCAATGGGTACGTGTGTGATCAAGAAAGAAATTATTTTGGAACGACCTGGAATTTCAAGCACTTCCATTCGACCTGATGGTAAAATTTTTGCAACCGCTGGCTGGGACCACAGAGTGAGGGTATATAACTATCGTAAAGGAAATGCTTTGGCAATATTGAAGTATCACCATGCAACGTGTAATGCCGTTTCTTATTCAGCTGATTGTAAGCTAATGGCATCTGCTTCAGAAGACACAACGGTGGCTCTATGGGAACTCTATCTTCCTCGAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000075 GO:0000151 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006282 GO:0006810 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0009555 GO:0009636 GO:0009663 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010044 GO:0010154 GO:0010162 GO:0010431 GO:0010496 GO:0010497 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022611 GO:0031323 GO:0031461 GO:0031570 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034330 GO:0035670 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045216 GO:0045786 GO:0045787 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048609 GO:0048700 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051179 GO:0051234 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0065007 GO:0071156 GO:0071158 GO:0071695 GO:0071840 GO:0072718 GO:0080008 GO:0080090 GO:0080134 GO:0080135 GO:0090068 GO:0090567 GO:0097327 GO:0097439 GO:0099402 GO:1902494 GO:1990234 GO:2000026 GO:2000280 GO:2001020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

446

Amino Acids

48.91

Weight (kDa)

6.67

Isoelectric Point (pI)

45.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	51 - 150	5.9e-07	CDC20/Fizzy WD40 domain
WD40	PF00400	68 - 104	9.3e-10	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	69 - 152	2.4e-11	WDR5 beta-propeller domain
WD40_Prp19	PF24814	69 - 150	1.4e-08	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	70 - 148	6e-12	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	71 - 253	1.4e-12	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	72 - 146	6.8e-07	WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	75 - 154	1.8e-10	TEP-1 second beta-propeller
Beta-prop_IFT140_1st	PF23383	77 - 159	5.8e-06	IFT140 first beta-propeller
Beta-prop_WDR3_1st	PF25173	235 - 358	6.5e-06	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	294 - 440	9.2e-16	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	294 - 440	1.1e-09	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	295 - 440	1.3e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	295 - 440	3.2e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	298 - 440	5.7e-14	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	299 - 439	9.7e-07	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	300 - 440	6.7e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	331 - 440	9.1e-07	MABP1/WDR62 second WD40 domain
Beta-prop_WDR5	PF25175	371 - 440	4.7e-14	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	372 - 440	1.3e-08	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	374 - 440	3.5e-06	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29860
fragaria_vesca	FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120 FvH4_2g39120
malus_domestica	MD08G1017100.v1.1 MD15G1016100.v1.1 MD15G1017900.v1.1 MD15G1018000.v1.1
prunus_persica	Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1 Prupe.1G369900_v2.0.a1
pyrus_communis	pycom08g01490 pycom15g01390
rosa_chinensis	RchiOBHm_Chr6g0272131 RchiOBHm_Chr6g0302231
rosa_laevigata	RLG00000011183 RLG00000013699
rosa_multiflora	Rmu_sc0013301.1_g000007 Rmu_sc0027601.1_g000001
rosa_roxburghii	Rroxscaffold_7G00165940 Rroxscaffold_7G00196580
rosa_rugosa	Rorug06G0065900 Rorug06G0313400.1
rosa_samantha	Rh6AG183100 Rh6BG184000 Rh6BG476300 Rh6CG184400 Rh6DG176100 Rh6DG425800
rosa_wichuraiana	Rw4G012510 Rw6G015760 Rw6G036890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	37, 915
AccII	CGCG	1 cut(s)	228
AciI	CCGC	4 cut(s)	194, 209, 226, 1158
AclI	AACGTT	1 cut(s)	633
AclWI	GGATC	3 cut(s)	753, 1052, 1065
AcoI	YGGCCR	1 cut(s)	191
AcsI	RAATTY	3 cut(s)	26, 1113, 1146
AcuI	CTGAAG	1 cut(s)	1272
AdeI	CACNNNGTG	1 cut(s)	1177
AfaI	GTAC	2 cut(s)	385, 1072
AfiI	CCNNNNNNNGG	9 cut(s)	37, 149, 156, 208, 412, 915, 957, 1067, 1164
AflIII	ACRYGT	2 cut(s)	1073, 1238
AgeI	ACCGGT	1 cut(s)	278
AgsI	TTSAA	7 cut(s)	443, 638, 671, 845, 1065, 1119, 1222
AjnI	CCWGG	3 cut(s)	405, 458, 1108
AleI	CACNNNNGTG	1 cut(s)	1301
AluBI	AGCT	7 cut(s)	134, 298, 341, 786, 1025, 1262, 1273
AluI	AGCT	7 cut(s)	134, 298, 341, 786, 1025, 1262, 1273
Alw21I	GWGCWC	1 cut(s)	75
Alw26I	GTCTC	2 cut(s)	181, 621
AlwI	GGATC	3 cut(s)	753, 1052, 1065
AlwNI	CAGNNNCTG	1 cut(s)	1025
Ama87I	CYCGRG	1 cut(s)	802
AoxI	GGCC	3 cut(s)	191, 220, 524
ApeKI	GCWGC	5 cut(s)	84, 177, 356, 786, 1025
ApoI	RAATTY	3 cut(s)	26, 1113, 1146
AsiGI	ACCGGT	1 cut(s)	278
Asp700I	GAANNNNTTC	1 cut(s)	975
AspLEI	GCGC	3 cut(s)	84, 149, 230
AspS9I	GGNCC	6 cut(s)	106, 199, 220, 241, 594, 1168
AsuC2I	CCSGG	3 cut(s)	803, 804, 936
AsuHPI	GGTGA	4 cut(s)	268, 529, 846, 1220
AvaI	CYCGRG	1 cut(s)	802
AvaII	GGWCC	5 cut(s)	106, 199, 241, 594, 1168
BaeI	ACNNNNGTAYC	2 cut(s)	1062, 1095
BamHI	GGATCC	1 cut(s)	1057
BauI	CACGAG	1 cut(s)	543
BbsI	GAAGAC	1 cut(s)	1299
Bbv12I	GWGCWC	1 cut(s)	75
BbvCI	CCTCAGC	2 cut(s)	123, 299
BbvI	GCAGC	5 cut(s)	71, 189, 368, 773, 1012
BccI	CCATC	7 cut(s)	284, 410, 563, 865, 896, 989, 1133
BceAI	ACGGC	3 cut(s)	206, 788, 1232
BciT130I	CCWGG	3 cut(s)	407, 460, 1110
BciVI	GTATCC	1 cut(s)	304
BclI	TGATCA	1 cut(s)	1080
BcnI	CCSGG	3 cut(s)	803, 804, 936
BcoDI	GTCTC	2 cut(s)	181, 621
BfaI	CTAG	2 cut(s)	614, 696
BfmI	CTRYAG	3 cut(s)	708, 756, 1026
BfoI	RGCGCY	2 cut(s)	85, 150
BfuI	GTATCC	1 cut(s)	304
BisI	GCNGC	6 cut(s)	85, 178, 194, 357, 787, 1026
BlsI	GCNGC	6 cut(s)	86, 179, 195, 358, 788, 1027
Bme1390I	CCNGG	6 cut(s)	407, 460, 803, 804, 936, 1110
Bme18I	GGWCC	5 cut(s)	106, 199, 241, 594, 1168
BmeT110I	CYCGRG	1 cut(s)	802
BmgT120I	GGNCC	6 cut(s)	106, 199, 220, 241, 594, 1168
BmiI	GGNNCC	9 cut(s)	107, 201, 221, 595, 799, 907, 942, 1059, 1169
BmrFI	CCNGG	6 cut(s)	407, 460, 803, 804, 936, 1110
BmrI	ACTGGG	1 cut(s)	197
BmsI	GCATC	3 cut(s)	331, 664, 1289
BmuI	ACTGGG	1 cut(s)	197
BoxI	GACNNNNGTC	1 cut(s)	953
BpiI	GAAGAC	1 cut(s)	1299
Bpu10I	CCTNAGC	3 cut(s)	123, 299, 651
BpuEI	CTTGAG	1 cut(s)	666
BpuMI	CCSGG	3 cut(s)	803, 804, 936
BsaAI	YACGTR	1 cut(s)	1074
BsaJI	CCNNGG	7 cut(s)	406, 458, 513, 801, 802, 816, 935
BsaWI	WCCGGW	1 cut(s)	278
BsaXI	ACNNNNNCTCC	2 cut(s)	215, 245
Bsc4I	CCNNNNNNNGG	9 cut(s)	37, 149, 156, 208, 412, 915, 957, 1067, 1164
Bse118I	RCCGGY	1 cut(s)	278
Bse1I	ACTGG	3 cut(s)	83, 203, 974
Bse3DI	GCAATG	1 cut(s)	1012
BseBI	CCWGG	3 cut(s)	407, 460, 1110
BseDI	CCNNGG	7 cut(s)	406, 458, 513, 801, 802, 816, 935
BseGI	GGATG	6 cut(s)	253, 295, 421, 655, 907, 1000
BseLI	CCNNNNNNNGG	9 cut(s)	37, 149, 156, 208, 412, 915, 957, 1067, 1164
BseMI	GCAATG	1 cut(s)	1012
BseMII	CTCAG	6 cut(s)	137, 206, 290, 642, 720, 1032
BseNI	ACTGG	3 cut(s)	83, 203, 974
BseRI	GAGGAG	1 cut(s)	111
BseX3I	CGGCCG	1 cut(s)	191
BseXI	GCAGC	5 cut(s)	71, 189, 368, 773, 1012
BseYI	CCCAGC	3 cut(s)	341, 659, 1164
Bsh1236I	CGCG	1 cut(s)	228
Bsh1285I	CGRYCG	1 cut(s)	194
BshFI	GGCC	3 cut(s)	193, 222, 526
BshTI	ACCGGT	1 cut(s)	278
BsiEI	CGRYCG	1 cut(s)	194
BsiHKAI	GWGCWC	1 cut(s)	75
BsiHKCI	CYCGRG	1 cut(s)	802
BsiSI	CCGG	5 cut(s)	150, 197, 279, 803, 935
BslFI	GGGAC	3 cut(s)	433, 607, 1181
BslI	CCNNNNNNNGG	9 cut(s)	37, 149, 156, 208, 412, 915, 957, 1067, 1164
BsmAI	GTCTC	2 cut(s)	181, 621
BsmBI	CGTCTC	1 cut(s)	181
BsmFI	GGGAC	3 cut(s)	433, 607, 1181
BsmI	GAATGC	1 cut(s)	848
BsnI	GGCC	3 cut(s)	193, 222, 526
BsoBI	CYCGRG	1 cut(s)	802
Bsp1286I	GDGCHC	1 cut(s)	75
Bsp143I	GATC	3 cut(s)	745, 1057, 1080
BspACI	CCGC	4 cut(s)	194, 209, 226, 1158
BspANI	GGCC	3 cut(s)	193, 222, 526
BspCNI	CTCAG	6 cut(s)	136, 207, 291, 643, 721, 1031
BspFNI	CGCG	1 cut(s)	228
BspHI	TCATGA	1 cut(s)	565
BspLI	GGNNCC	9 cut(s)	107, 201, 221, 595, 799, 907, 942, 1059, 1169
BspMAI	CTGCAG	2 cut(s)	760, 1030
BspPI	GGATC	3 cut(s)	753, 1052, 1065
BsrDI	GCAATG	1 cut(s)	1012
BsrFI	RCCGGY	1 cut(s)	278
BsrI	ACTGG	3 cut(s)	83, 203, 974
BssAI	RCCGGY	1 cut(s)	278
BssECI	CCNNGG	7 cut(s)	406, 458, 513, 801, 802, 816, 935
BssMI	GATC	3 cut(s)	745, 1057, 1080
BssSI	CACGAG	1 cut(s)	543
BssT1I	CCWWGG	2 cut(s)	513, 816
Bst2BI	CACGAG	1 cut(s)	543
Bst2UI	CCWGG	3 cut(s)	407, 460, 1110
Bst4CI	ACNGT	3 cut(s)	331, 424, 1303
Bst6I	CTCTTC	2 cut(s)	55, 515
BstBAI	YACGTR	1 cut(s)	1074
BstC8I	GCNNGC	3 cut(s)	182, 296, 1162
BstDEI	CTNAG	7 cut(s)	123, 215, 299, 651, 729, 980, 1018
BstENI	CCTNNNNNAGG	1 cut(s)	955
BstF5I	GGATG	6 cut(s)	253, 295, 421, 655, 907, 1000
BstFNI	CGCG	1 cut(s)	228
BstH2I	RGCGCY	2 cut(s)	85, 150
BstHHI	GCGC	3 cut(s)	84, 149, 230
BstKTI	GATC	3 cut(s)	748, 1060, 1083
BstMAI	GTCTC	2 cut(s)	181, 621
BstMBI	GATC	3 cut(s)	745, 1057, 1080
BstMCI	CGRYCG	1 cut(s)	194
BstMWI	GCNNNNNNNGC	5 cut(s)	79, 190, 377, 386, 845
BstNI	CCWGG	3 cut(s)	407, 460, 1110
BstPAI	GACNNNNGTC	1 cut(s)	953
BstSCI	CCNGG	6 cut(s)	405, 458, 801, 802, 934, 1108
BstSFI	CTRYAG	3 cut(s)	708, 756, 1026
BstUI	CGCG	1 cut(s)	228
BstV1I	GCAGC	5 cut(s)	71, 189, 368, 773, 1012
BstV2I	GAAGAC	1 cut(s)	1299
BstX2I	RGATCY	2 cut(s)	745, 1057
BstYI	RGATCY	2 cut(s)	745, 1057
BstZI	CGGCCG	1 cut(s)	191
BsuI	GTATCC	1 cut(s)	304
BsuRI	GGCC	3 cut(s)	193, 222, 526
BtsCI	GGATG	6 cut(s)	253, 295, 421, 655, 907, 1000
BtsIMutI	CAGTG	2 cut(s)	210, 597
Cac8I	GCNNGC	3 cut(s)	182, 296, 1162
CaiI	CAGNNNCTG	1 cut(s)	1025
CciI	TCATGA	1 cut(s)	565
CfoI	GCGC	3 cut(s)	84, 149, 230
Cfr10I	RCCGGY	1 cut(s)	278
Cfr13I	GGNCC	6 cut(s)	106, 199, 220, 241, 594, 1168
Cfr9I	CCCGGG	1 cut(s)	802
Csp6I	GTAC	2 cut(s)	384, 1071
CspAI	ACCGGT	1 cut(s)	278
CviAII	CATG	7 cut(s)	238, 362, 566, 835, 910, 1002, 1232
CviQI	GTAC	2 cut(s)	384, 1071
DdeI	CTNAG	7 cut(s)	123, 215, 299, 651, 729, 980, 1018
DpnI	GATC	3 cut(s)	747, 1059, 1082
DpnII	GATC	3 cut(s)	745, 1057, 1080
DraIII	CACNNNGTG	1 cut(s)	1177
EaeI	YGGCCR	1 cut(s)	191
EagI	CGGCCG	1 cut(s)	191
Eam1104I	CTCTTC	2 cut(s)	55, 515
EarI	CTCTTC	2 cut(s)	55, 515
EclXI	CGGCCG	1 cut(s)	191
Eco130I	CCWWGG	2 cut(s)	513, 816
Eco147I	AGGCCT	1 cut(s)	526
Eco47I	GGWCC	5 cut(s)	106, 199, 241, 594, 1168
Eco52I	CGGCCG	1 cut(s)	191
Eco57I	CTGAAG	1 cut(s)	1272
Eco88I	CYCGRG	1 cut(s)	802
EcoNI	CCTNNNNNAGG	1 cut(s)	955
EcoO109I	RGGNCCY	1 cut(s)	106
EcoRII	CCWGG	3 cut(s)	405, 458, 1108
EcoT14I	CCWWGG	2 cut(s)	513, 816
ErhI	CCWWGG	2 cut(s)	513, 816
Esp3I	CGTCTC	1 cut(s)	181
FaeI	CATG	7 cut(s)	241, 365, 569, 838, 913, 1005, 1235
FaqI	GGGAC	3 cut(s)	433, 607, 1181
FatI	CATG	7 cut(s)	237, 361, 565, 834, 909, 1001, 1231
FbaI	TGATCA	1 cut(s)	1080
Fnu4HI	GCNGC	6 cut(s)	85, 178, 194, 357, 787, 1026
FokI	GGATG	6 cut(s)	240, 302, 428, 642, 914, 1007
Fsp4HI	GCNGC	6 cut(s)	85, 178, 194, 357, 787, 1026
FspBI	CTAG	2 cut(s)	614, 696
GlaI	GCGC	3 cut(s)	83, 148, 229
GluI	GCNGC	6 cut(s)	85, 178, 194, 357, 787, 1026
GsaI	CCCAGC	3 cut(s)	345, 663, 1168
HaeII	RGCGCY	2 cut(s)	85, 150
HaeIII	GGCC	3 cut(s)	193, 222, 526
HapII	CCGG	5 cut(s)	150, 197, 279, 803, 935
HhaI	GCGC	3 cut(s)	84, 149, 230
Hin1II	CATG	7 cut(s)	241, 365, 569, 838, 913, 1005, 1235
Hin6I	GCGC	3 cut(s)	82, 147, 228
HinP1I	GCGC	3 cut(s)	82, 147, 228
HinfI	GANTC	4 cut(s)	463, 643, 863, 873
HpaII	CCGG	5 cut(s)	150, 197, 279, 803, 935
HphI	GGTGA	4 cut(s)	268, 529, 846, 1220
Hpy166II	GTNNAC	4 cut(s)	100, 276, 506, 550
Hpy188I	TCNGA	8 cut(s)	171, 216, 289, 327, 730, 862, 970, 1291
Hpy188III	TCNNGA	7 cut(s)	481, 566, 614, 683, 696, 1020, 1084
Hpy8I	GTNNAC	4 cut(s)	100, 276, 506, 550
HpyAV	CCTTC	5 cut(s)	67, 267, 437, 632, 1071
HpyCH4III	ACNGT	3 cut(s)	331, 424, 1303
HpyCH4IV	ACGT	3 cut(s)	633, 1073, 1238
HpyCH4V	TGCA	9 cut(s)	349, 356, 758, 770, 834, 1005, 1028, 1154, 1235
HpyF10VI	GCNNNNNNNGC	5 cut(s)	79, 190, 377, 386, 845
HpyF3I	CTNAG	7 cut(s)	123, 215, 299, 651, 729, 980, 1018
HpySE526I	ACGT	3 cut(s)	633, 1073, 1238
Hsp92II	CATG	7 cut(s)	241, 365, 569, 838, 913, 1005, 1235
HspAI	GCGC	3 cut(s)	82, 147, 228
Ksp22I	TGATCA	1 cut(s)	1080
Kzo9I	GATC	3 cut(s)	745, 1057, 1080
LmnI	GCTCC	2 cut(s)	718, 1022
Lsp1109I	GCAGC	5 cut(s)	71, 189, 368, 773, 1012
LweI	GCATC	3 cut(s)	331, 664, 1289
MaeI	CTAG	2 cut(s)	614, 696
MaeII	ACGT	3 cut(s)	633, 1073, 1238
MaeIII	GTNAC	1 cut(s)	472
MalI	GATC	3 cut(s)	747, 1059, 1082
MboI	GATC	3 cut(s)	745, 1057, 1080
MboII	GAAGA	8 cut(s)	42, 45, 532, 617, 683, 804, 1304, 1316
MflI	RGATCY	2 cut(s)	745, 1057
MhlI	GDGCHC	1 cut(s)	75
MluCI	AATT	5 cut(s)	26, 689, 1092, 1113, 1146
MlyI	GAGTC	1 cut(s)	457
MmeI	TCCRAC	1 cut(s)	55
MroXI	GAANNNNTTC	1 cut(s)	975
MseI	TTAA	2 cut(s)	426, 1336
MslI	CAYNNNNRTG	1 cut(s)	1301
MspA1I	CMGCKG	3 cut(s)	341, 1160, 1262
MspI	CCGG	5 cut(s)	150, 197, 279, 803, 935
MspR9I	CCNGG	6 cut(s)	407, 460, 803, 804, 936, 1110
Mva1269I	GAATGC	1 cut(s)	848
MvaI	CCWGG	3 cut(s)	407, 460, 1110
MvnI	CGCG	1 cut(s)	228
MwoI	GCNNNNNNNGC	5 cut(s)	79, 190, 377, 386, 845
NciI	CCSGG	3 cut(s)	803, 804, 936
NdeII	GATC	3 cut(s)	745, 1057, 1080
NlaIII	CATG	7 cut(s)	241, 365, 569, 838, 913, 1005, 1235
NlaIV	GGNNCC	9 cut(s)	107, 201, 221, 595, 799, 907, 942, 1059, 1169
OliI	CACNNNNGTG	1 cut(s)	1301
PagI	TCATGA	1 cut(s)	565
PceI	AGGCCT	1 cut(s)	526
PctI	GAATGC	1 cut(s)	848
PdmI	GAANNNNTTC	1 cut(s)	975
PfeI	GAWTC	3 cut(s)	643, 863, 873
PflFI	GACNNNGTC	1 cut(s)	466
PflMI	CCANNNNNTGG	2 cut(s)	37, 915
PinAI	ACCGGT	1 cut(s)	278
PkrI	GCNGC	6 cut(s)	86, 179, 195, 358, 788, 1027
PleI	GAGTC	1 cut(s)	457
PpsI	GAGTC	1 cut(s)	457
Ppu21I	YACGTR	1 cut(s)	1074
PpuMI	RGGWCCY	1 cut(s)	106
PshAI	GACNNNNGTC	1 cut(s)	953
Psp1406I	AACGTT	1 cut(s)	633
Psp5II	RGGWCCY	1 cut(s)	106
Psp6I	CCWGG	3 cut(s)	405, 458, 1108
PspFI	CCCAGC	3 cut(s)	341, 659, 1164
PspGI	CCWGG	3 cut(s)	405, 458, 1108
PspN4I	GGNNCC	9 cut(s)	107, 201, 221, 595, 799, 907, 942, 1059, 1169
PspPI	GGNCC	6 cut(s)	106, 199, 220, 241, 594, 1168
PspPPI	RGGWCCY	1 cut(s)	106
PstI	CTGCAG	2 cut(s)	760, 1030
PstNI	CAGNNNCTG	1 cut(s)	1025
PsuI	RGATCY	2 cut(s)	745, 1057
PsyI	GACNNNGTC	1 cut(s)	466
PvuII	CAGCTG	2 cut(s)	341, 1262
RsaI	GTAC	2 cut(s)	385, 1072
RsaNI	GTAC	2 cut(s)	384, 1071
RseI	CAYNNNNRTG	1 cut(s)	1301
SaqAI	TTAA	2 cut(s)	426, 1336
SatI	GCNGC	6 cut(s)	85, 178, 194, 357, 787, 1026
Sau3AI	GATC	3 cut(s)	745, 1057, 1080
Sau96I	GGNCC	6 cut(s)	106, 199, 220, 241, 594, 1168
SchI	GAGTC	1 cut(s)	457
ScrFI	CCNGG	6 cut(s)	407, 460, 803, 804, 936, 1110
SduI	GDGCHC	1 cut(s)	75
SfaNI	GCATC	3 cut(s)	331, 664, 1289
SfcI	CTRYAG	3 cut(s)	708, 756, 1026
SinI	GGWCC	5 cut(s)	106, 199, 241, 594, 1168
SmaI	CCCGGG	1 cut(s)	804
SmiMI	CAYNNNNRTG	1 cut(s)	1301
SmlI	CTYRAG	1 cut(s)	681
SmoI	CTYRAG	1 cut(s)	681
Sse9I	AATT	5 cut(s)	26, 689, 1092, 1113, 1146
SseBI	AGGCCT	1 cut(s)	526
SsiI	CCGC	4 cut(s)	194, 209, 226, 1158
SspI	AATATT	1 cut(s)	1218
SspMI	CTAG	2 cut(s)	614, 696
StuI	AGGCCT	1 cut(s)	526
StyD4I	CCNGG	6 cut(s)	405, 458, 801, 802, 934, 1108
StyI	CCWWGG	2 cut(s)	513, 816
TaaI	ACNGT	3 cut(s)	331, 424, 1303
TaiI	ACGT	3 cut(s)	636, 1076, 1241
TaqI	TCGA	2 cut(s)	1132, 1330
TasI	AATT	5 cut(s)	26, 689, 1092, 1113, 1146
TatI	WGTACW	1 cut(s)	383
TauI	GCSGC	1 cut(s)	196
TfiI	GAWTC	3 cut(s)	643, 863, 873
Tru1I	TTAA	2 cut(s)	426, 1336
Tru9I	TTAA	2 cut(s)	426, 1336
TscAI	CASTG	2 cut(s)	210, 604
TseI	GCWGC	5 cut(s)	84, 177, 356, 786, 1025
TspDTI	ATGAA	6 cut(s)	242, 273, 554, 618, 702, 953
TspGWI	ACGGA	2 cut(s)	223, 456
TspMI	CCCGGG	1 cut(s)	802
TspRI	CASTG	2 cut(s)	210, 604
Tth111I	GACNNNGTC	1 cut(s)	466
Van91I	CCANNNNNTGG	2 cut(s)	37, 915
VpaK11BI	GGWCC	5 cut(s)	106, 199, 241, 594, 1168
XagI	CCTNNNNNAGG	1 cut(s)	955
XapI	RAATTY	3 cut(s)	26, 1113, 1146
XbaI	TCTAGA	2 cut(s)	613, 695
XcmI	CCANNNNNNNNNTGG	1 cut(s)	413
XmaI	CCCGGG	1 cut(s)	802
XmnI	GAANNNNTTC	1 cut(s)	975
XspI	CTAG	2 cut(s)	614, 696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.