pycom15g01710

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008497
Physical Location & Seq
Reverse (-)
993822 .. 994667
846 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGGCTCTCCCTCAGCTCATCCCCTCCTCTTCCTCCTCCTCCCTCCCCCATAAACCCACCTTCAACCCCAATCCCCTCCCCTCCCGCTTAAACCCTAACCCTAATATCCTCTCCCCCCGCCTCCACCGCCGCCGCCAACTACCCGGATCCCTCCAATGCACAGCCTCCTCATTCTCTGAGCGCCACCCCACCAATCAGCCCAAATCAGGGGACGTCGTCGAGCTCCCCATTTTCCCCTTGCCCCTCGTCCTCTTCCCGGGCGCCATCCTCCCCCTCCAGATATTCGAGTTCCGCTACCGCATGATGATGCACTCCCTCCTCCAGTCCGATCTCCGCTTCGGCGTAATTTACAGCGACACCGTCTCCGGTACCGCCGACGTCGGCTGCGTCGGCGAGGTCGTCAAGCACGAGCGCCTTGTCGACGACCGGTTCTTCCTCATCTGCAAGGGCCAGGAGCGGTTTCGCATCACCGACCTCGTCCGCACCAAACCCTACCTGGTGGCGGAGGTCAAGTGGCTTGAAGACCGGCCGTCGAGCGACGGCGAGGACGACCTGGAAGCGTTGGCGAACGAGGTCGAGTCGCATATGAAGGATGTGATTCGGTTGTCGAATCGGTTGGGCGGGAAGCCGGAGAAGGATGTGCAGGACCTGCGGCGGAACCTATTTCCGACGCTATTTTCGTTTTTTGTTGGGAGCACGTTTGAAGGGGCGCCGAGAGAGCAGCAGGCGTTGCTGGAATTGGAGGACACGGCGGCGCGGTTGAAGAGAGAGAAGGAGACGCTGAGGAACACACTTAATTACTTGACGGCAGCCTCCGCCGTCAAAGACGTCTTCCCGTCGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

282

Amino Acids

31.71

Weight (kDa)

7.03

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 74 - 261 3.5e-29 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 216, 381, 831
Acc36I ACCTGC 1 cut(s) 657
Acc65I GGTACC 1 cut(s) 368
AccB1I GGYRCC 3 cut(s) 260, 368, 709
AccBSI CCGCTC 1 cut(s) 457
AccI GTMKAC 1 cut(s) 420
AccII CGCG 1 cut(s) 757
AclWI GGATC 2 cut(s) 141, 154
AcoI YGGCCR 1 cut(s) 527
AcyI GRCGYC 5 cut(s) 213, 261, 378, 710, 828
AfaI GTAC 1 cut(s) 370
AfiI CCNNNNNNNGG 4 cut(s) 206, 207, 256, 502
AgeI ACCGGT 1 cut(s) 426
AgsI TTSAA 4 cut(s) 64, 521, 704, 763
AhdI GACNNNNNGTC 1 cut(s) 529
AjnI CCWGG 3 cut(s) 450, 495, 552
AluBI AGCT 2 cut(s) 16, 223
AluI AGCT 2 cut(s) 16, 223
Alw21I GWGCWC 2 cut(s) 225, 698
Alw26I GTCTC 2 cut(s) 367, 770
AlwI GGATC 2 cut(s) 141, 154
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 2 cut(s) 448, 527
ApeKI GCWGC 3 cut(s) 384, 721, 809
ArsI GACNNNNNNTTYG 2 cut(s) 661, 693
AsiGI ACCGGT 1 cut(s) 426
Asp718I GGTACC 1 cut(s) 368
AspLEI GCGC 5 cut(s) 183, 263, 414, 712, 757
AspS9I GGNCC 2 cut(s) 448, 646
AsuC2I CCSGG 3 cut(s) 144, 257, 258
AsuHPI GGTGA 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 1 cut(s) 646
BamHI GGATCC 1 cut(s) 146
BanI GGYRCC 3 cut(s) 260, 368, 709
BanII GRGCYC 1 cut(s) 225
BauI CACGAG 1 cut(s) 407
BbsI GAAGAC 2 cut(s) 528, 823
Bbv12I GWGCWC 2 cut(s) 225, 698
BbvCI CCTCAGC 2 cut(s) 12, 782
BbvI GCAGC 3 cut(s) 371, 733, 821
BccI CCATC 1 cut(s) 272
BceAI ACGGC 5 cut(s) 514, 556, 765, 803, 822
BciT130I CCWGG 3 cut(s) 452, 497, 554
BcnI CCSGG 3 cut(s) 144, 257, 258
BcoDI GTCTC 2 cut(s) 367, 770
BfoI RGCGCY 4 cut(s) 184, 264, 415, 713
BfuAI ACCTGC 1 cut(s) 657
BisI GCNGC 7 cut(s) 130, 133, 385, 653, 722, 753, 810
BlsI GCNGC 7 cut(s) 131, 134, 386, 654, 723, 754, 811
Bme1390I CCNGG 6 cut(s) 144, 257, 258, 452, 497, 554
Bme18I GGWCC 1 cut(s) 646
BmeRI GACNNNNNGTC 1 cut(s) 529
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 2 cut(s) 448, 646
BmiI GGNNCC 5 cut(s) 148, 262, 370, 659, 711
BmrFI CCNGG 6 cut(s) 144, 257, 258, 452, 497, 554
BmsI GCATC 2 cut(s) 297, 474
BpiI GAAGAC 2 cut(s) 528, 823
BpmI CTGGAG 2 cut(s) 260, 305
Bpu10I CCTNAGC 2 cut(s) 12, 782
BpuMI CCSGG 3 cut(s) 144, 257, 258
BsaHI GRCGYC 5 cut(s) 213, 261, 378, 710, 828
BsaJI CCNNGG 1 cut(s) 256
BsaWI WCCGGW 2 cut(s) 365, 426
BsaXI ACNNNNNCTCC 4 cut(s) 347, 377, 497, 527
Bsc4I CCNNNNNNNGG 4 cut(s) 206, 207, 256, 502
Bse118I RCCGGY 2 cut(s) 426, 525
Bse1I ACTGG 1 cut(s) 322
BseBI CCWGG 3 cut(s) 452, 497, 554
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 4 cut(s) 18, 264, 598, 643
BseLI CCNNNNNNNGG 4 cut(s) 206, 207, 256, 502
BseMII CTCAG 3 cut(s) 26, 168, 773
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 5 cut(s) 16, 25, 28, 157, 308
BseX3I CGGCCG 1 cut(s) 527
BseXI GCAGC 3 cut(s) 371, 733, 821
BsgI GTGCAG 1 cut(s) 662
Bsh1236I CGCG 1 cut(s) 757
Bsh1285I CGRYCG 2 cut(s) 427, 530
BshFI GGCC 2 cut(s) 450, 529
BshNI GGYRCC 3 cut(s) 260, 368, 709
BshTI ACCGGT 1 cut(s) 426
BsiEI CGRYCG 2 cut(s) 427, 530
BsiHKAI GWGCWC 2 cut(s) 225, 698
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 6 cut(s) 144, 257, 366, 427, 526, 629
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 4 cut(s) 206, 207, 256, 502
BsmAI GTCTC 2 cut(s) 367, 770
BsmBI CGTCTC 2 cut(s) 367, 770
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 2 cut(s) 450, 529
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 225, 698
Bsp143I GATC 2 cut(s) 146, 328
BspANI GGCC 2 cut(s) 450, 529
BspCNI CTCAG 3 cut(s) 25, 169, 774
BspFNI CGCG 1 cut(s) 757
BspLI GGNNCC 5 cut(s) 148, 262, 370, 659, 711
BspMI ACCTGC 1 cut(s) 657
BspPI GGATC 2 cut(s) 141, 154
BspT107I GGYRCC 3 cut(s) 260, 368, 709
BsrBI CCGCTC 1 cut(s) 457
BsrFI RCCGGY 2 cut(s) 426, 525
BsrI ACTGG 1 cut(s) 322
BssAI RCCGGY 2 cut(s) 426, 525
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 2 cut(s) 146, 328
BssNI GRCGYC 5 cut(s) 213, 261, 378, 710, 828
BssSI CACGAG 1 cut(s) 407
Bst2BI CACGAG 1 cut(s) 407
Bst2UI CCWGG 3 cut(s) 452, 497, 554
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 3 cut(s) 34, 257, 758
BstACI GRCGYC 5 cut(s) 213, 261, 378, 710, 828
BstAPI GCANNNNNTGC 2 cut(s) 649, 730
BstC8I GCNNGC 1 cut(s) 726
BstDEI CTNAG 3 cut(s) 12, 177, 782
BstF5I GGATG 4 cut(s) 18, 264, 598, 643
BstFNI CGCG 1 cut(s) 757
BstH2I RGCGCY 4 cut(s) 184, 264, 415, 713
BstHHI GCGC 5 cut(s) 183, 263, 414, 712, 757
BstKTI GATC 2 cut(s) 149, 331
BstMAI GTCTC 2 cut(s) 367, 770
BstMBI GATC 2 cut(s) 146, 328
BstMCI CGRYCG 2 cut(s) 427, 530
BstMWI GCNNNNNNNGC 6 cut(s) 126, 390, 649, 718, 730, 815
BstNI CCWGG 3 cut(s) 452, 497, 554
BstSCI CCNGG 6 cut(s) 142, 255, 256, 450, 495, 552
BstUI CGCG 1 cut(s) 757
BstV1I GCAGC 3 cut(s) 371, 733, 821
BstV2I GAAGAC 2 cut(s) 528, 823
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BstZI CGGCCG 1 cut(s) 527
BsuRI GGCC 2 cut(s) 450, 529
BtsCI GGATG 4 cut(s) 18, 264, 598, 643
BveI ACCTGC 1 cut(s) 657
Cac8I GCNNGC 1 cut(s) 726
CaiI CAGNNNCTG 1 cut(s) 649
CfoI GCGC 5 cut(s) 183, 263, 414, 712, 757
Cfr10I RCCGGY 2 cut(s) 426, 525
Cfr13I GGNCC 2 cut(s) 448, 646
Cfr9I CCCGGG 1 cut(s) 256
CseI GACGC 3 cut(s) 376, 679, 787
CsiI ACCWGGT 1 cut(s) 495
Csp6I GTAC 1 cut(s) 369
CspAI ACCGGT 1 cut(s) 426
CviAII CATG 1 cut(s) 301
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 3 cut(s) 12, 177, 782
DinI GGCGCC 2 cut(s) 262, 711
DpnI GATC 2 cut(s) 148, 330
DpnII GATC 2 cut(s) 146, 328
DriI GACNNNNNGTC 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 527
EagI CGGCCG 1 cut(s) 527
Eam1104I CTCTTC 3 cut(s) 34, 257, 758
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 3 cut(s) 34, 257, 758
EciI GGCGGA 3 cut(s) 518, 670, 805
Ecl136II GAGCTC 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 527
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 646
Eco52I CGGCCG 1 cut(s) 527
Eco53kI GAGCTC 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 646
EcoRII CCWGG 3 cut(s) 450, 495, 552
EcoT38I GRGCYC 1 cut(s) 225
EgeI GGCGCC 2 cut(s) 262, 711
EheI GGCGCC 2 cut(s) 262, 711
Esp3I CGTCTC 2 cut(s) 367, 770
FaeI CATG 1 cut(s) 304
FaiI YATR 4 cut(s) 51, 302, 585, 587
FaqI GGGAC 1 cut(s) 224
FatI CATG 1 cut(s) 300
FauI CCCGC 3 cut(s) 92, 125, 614
FauNDI CATATG 1 cut(s) 585
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 7 cut(s) 130, 133, 385, 653, 722, 753, 810
FokI GGATG 4 cut(s) 5, 251, 605, 650
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 7 cut(s) 130, 133, 385, 653, 722, 753, 810
GlaI GCGC 5 cut(s) 182, 262, 413, 711, 756
GluI GCNGC 7 cut(s) 130, 133, 385, 653, 722, 753, 810
GsuI CTGGAG 2 cut(s) 260, 305
HaeII RGCGCY 4 cut(s) 184, 264, 415, 713
HaeIII GGCC 2 cut(s) 450, 529
HapII CCGG 6 cut(s) 144, 257, 366, 427, 526, 629
HgaI GACGC 3 cut(s) 376, 679, 787
HhaI GCGC 5 cut(s) 183, 263, 414, 712, 757
Hin1I GRCGYC 5 cut(s) 213, 261, 378, 710, 828
Hin1II CATG 1 cut(s) 304
Hin6I GCGC 5 cut(s) 181, 261, 412, 710, 755
HinP1I GCGC 5 cut(s) 181, 261, 412, 710, 755
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 3 cut(s) 578, 598, 610
HpaII CCGG 6 cut(s) 144, 257, 366, 427, 526, 629
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 3 cut(s) 178, 328, 669
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 5 cut(s) 70, 583, 628, 698, 766
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 4 cut(s) 213, 378, 698, 828
HpyCH4V TGCA 4 cut(s) 159, 310, 444, 643
HpyF10VI GCNNNNNNNGC 6 cut(s) 126, 390, 649, 718, 730, 815
HpyF3I CTNAG 3 cut(s) 12, 177, 782
HpySE526I ACGT 4 cut(s) 213, 378, 698, 828
Hsp92I GRCGYC 5 cut(s) 213, 261, 378, 710, 828
Hsp92II CATG 1 cut(s) 304
HspAI GCGC 5 cut(s) 181, 261, 412, 710, 755
KasI GGCGCC 2 cut(s) 260, 709
KpnI GGTACC 1 cut(s) 372
Kzo9I GATC 2 cut(s) 146, 328
LmnI GCTCC 3 cut(s) 228, 454, 693
Lsp1109I GCAGC 3 cut(s) 371, 733, 821
LweI GCATC 2 cut(s) 297, 474
MabI ACCWGGT 1 cut(s) 495
MaeII ACGT 4 cut(s) 213, 378, 698, 828
MalI GATC 2 cut(s) 148, 330
MbiI CCGCTC 1 cut(s) 457
MboI GATC 2 cut(s) 146, 328
MboII GAAGA 6 cut(s) 21, 244, 424, 533, 775, 823
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 2 cut(s) 225, 698
MluCI AATT 3 cut(s) 345, 737, 796
Mly113I GGCGCC 2 cut(s) 261, 710
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 1 cut(s) 692
MseI TTAA 2 cut(s) 89, 795
MslI CAYNNNNRTG 1 cut(s) 305
MspI CCGG 6 cut(s) 144, 257, 366, 427, 526, 629
MspR9I CCNGG 6 cut(s) 144, 257, 258, 452, 497, 554
MvaI CCWGG 3 cut(s) 452, 497, 554
MvnI CGCG 1 cut(s) 757
MwoI GCNNNNNNNGC 6 cut(s) 126, 390, 649, 718, 730, 815
NarI GGCGCC 2 cut(s) 261, 710
NciI CCSGG 3 cut(s) 144, 257, 258
NdeI CATATG 1 cut(s) 585
NdeII GATC 2 cut(s) 146, 328
NlaIII CATG 1 cut(s) 304
NlaIV GGNNCC 5 cut(s) 148, 262, 370, 659, 711
NmeAIII GCCGAG 1 cut(s) 738
PcsI WCGNNNNNNNCGW 5 cut(s) 384, 396, 405, 546, 677
PfeI GAWTC 2 cut(s) 598, 610
PflFI GACNNNGTC 3 cut(s) 215, 359, 476
PinAI ACCGGT 1 cut(s) 426
PkrI GCNGC 7 cut(s) 131, 134, 386, 654, 723, 754, 811
PleI GAGTC 1 cut(s) 586
PluTI GGCGCC 2 cut(s) 264, 713
PpsI GAGTC 1 cut(s) 586
PpuMI RGGWCCY 1 cut(s) 646
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 3 cut(s) 450, 495, 552
PspGI CCWGG 3 cut(s) 450, 495, 552
PspN4I GGNNCC 5 cut(s) 148, 262, 370, 659, 711
PspPI GGNCC 2 cut(s) 448, 646
PspPPI RGGWCCY 1 cut(s) 646
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 146
PsyI GACNNNGTC 3 cut(s) 215, 359, 476
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
RseI CAYNNNNRTG 1 cut(s) 305
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 2 cut(s) 89, 795
SatI GCNGC 7 cut(s) 130, 133, 385, 653, 722, 753, 810
Sau3AI GATC 2 cut(s) 146, 328
Sau96I GGNCC 2 cut(s) 448, 646
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 6 cut(s) 144, 257, 258, 452, 497, 554
SduI GDGCHC 2 cut(s) 225, 698
SexAI ACCWGGT 1 cut(s) 495
SfaNI GCATC 2 cut(s) 297, 474
SfoI GGCGCC 2 cut(s) 262, 711
SinI GGWCC 1 cut(s) 646
SmaI CCCGGG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 305
Sse9I AATT 3 cut(s) 345, 737, 796
SspDI GGCGCC 2 cut(s) 260, 709
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 6 cut(s) 142, 255, 256, 450, 495, 552
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 4 cut(s) 216, 381, 701, 831
TaqI TCGA 6 cut(s) 219, 285, 420, 533, 576, 608
TasI AATT 3 cut(s) 345, 737, 796
TauI GCSGC 4 cut(s) 132, 135, 655, 755
TfiI GAWTC 2 cut(s) 598, 610
Tru1I TTAA 2 cut(s) 89, 795
Tru9I TTAA 2 cut(s) 89, 795
TseI GCWGC 3 cut(s) 384, 721, 809
TspDTI ATGAA 1 cut(s) 602
TspMI CCCGGG 1 cut(s) 256
Tth111I GACNNNGTC 3 cut(s) 215, 359, 476
VpaK11BI GGWCC 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmaI CCCGGG 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 420
ZraI GACGTC 3 cut(s) 214, 379, 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.