pycom15g03540

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009882
Physical Location & Seq
Forward (+)
2171432 .. 2173757
2326 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
ATGGCGAGCAGGAGAGCCACCACGACACTCACCAAGTCGGCCCTAATTCTGGCGTCCCTGAACGCCACTGAATCCTCGAGAATCCCTCTCTCTCGGAGTCGGGCAATCGCCGTCGCCGCATCTGCCCGGACCTTCGGCAGCTCTGCCGTTCCCTTCTCTCGCCCCAGCTTTGTTCCCGAGAGCAGAAATGTAGCTTCGGCCAAGTATTTGGCAAGCGCCTTCACTCGCAGCTTCCACTCTACGACTCCCAATTTCTCCTCCGCCACGAGCTCCTCTCAGGCCAATCAAAATGAGTTAACTGAGATGGCGTGGGAGGACATTGTTGGTGCCGTTGACGCCGCCCGAAATAGCAAGCAGCAGGTGGTGGAGTCTGAGCACTTGATGAAAGCGCTTTTGGAGCAGAAAGATGGTTTGGCCCGGAGAATTTTCACGAAGGCTGGGCTCGACAACACTACGGTTCTTCAAGCAACTGACCACTTCATTGATAAGCAACCCAAGGTTGTGTTGTGGCAGGTGACGGGTGGCACCAGTGGCCCTATTATGGGTTCACATCTGGTTGGCTTGTTGGACAATGCTCGGCGGCAGAAGAAAGATATGAAAGATGATTTCGTTTCGGTGGAGCACCTTGTGTTGGCGTTTCAGGCCGATACGCGGTTTGGGCAGCAATTGTTCAGAAACCTCCAGCTTAGTGATAAGGATTTGAAGGAGGCTGTTAAAGATGTTCGTGGCAACCAGAGAGTCACTGATCAAAATCCTGAAGGAAAATATGAGGCCTTGGATAAGTACGGGAATGATTTGACTGAACTCACTAGGCGTGGCAAACTTGATCCTGTCATAGGCCGAGATGATGAAATACGGCAGTGTATCCAGATATTGTCTAGGAGAACGAAGAATAATCCTGTTATTATTGGTGAGCCTGGTTTGATTTCTTTAGATATGGGATCGTTGGTTGCTGGTGCCAAATTTTGTGGGGATTTTGAGGAAAGATTGAAAGCTGTTCTGAAGGAAGTTACTGCTTCCAATGGGCAGATTTTTTTATTTATTGATGAAATTCATACTGTTGTAGGAGCTGTCGTATTATCTCCTGATGTCCTTGTTAATGATTCTGTTAGGGGCTACAACTGGTGCGATGGATGCTGGGAACTTGTTGAAACCAATGCTTGGGCGATTGAATGA

Protein Analysis

392

Amino Acids

42.97

Weight (kDa)

6.63

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 99 - 225 2.2e-26 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25140
fragaria_vesca FvH4_2g40970 FvH4_2g40970 FvH4_2g40970 FvH4_2g40970
malus_domestica MD08G1058100.v1.1 MD15G1038200.v1.1
prunus_persica Prupe.1G392200_v2.0.a1
pyrus_communis pycom08g04820 pycom15g03540
rosa_chinensis RchiOBHm_Chr6g0299601
rosa_laevigata RLG00000011406
rosa_multiflora Rmu_sc0002895.1_g000001
rosa_roxburghii Rroxscaffold_7G00168520
rosa_rugosa Rorug06G0290800 Rorug06G0290900
rosa_samantha Rh6AG401700 Rh6BG410100 Rh6CG415700 Rh6DG402900
rosa_wichuraiana Rw6G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 349, 502
Acc36I ACCTGC 2 cut(s) 349, 502
AccB1I GGYRCC 3 cut(s) 326, 524, 956
AccB7I CCANNNNNTGG 1 cut(s) 1161
AccII CGCG 1 cut(s) 652
AciI CCGC 5 cut(s) 117, 261, 339, 580, 652
AclWI GGATC 2 cut(s) 821, 949
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 3 cut(s) 423, 962, 1050
AcuI CTGAAG 2 cut(s) 777, 1022
AcyI GRCGYC 2 cut(s) 53, 336
AdeI CACNNNGTG 1 cut(s) 628
AfaI GTAC 1 cut(s) 785
AfeI AGCGCT 1 cut(s) 390
AfiI CCNNNNNNNGG 7 cut(s) 49, 541, 542, 631, 651, 836, 1161
AgsI TTSAA 5 cut(s) 464, 703, 991, 1151, 1172
AjnI CCWGG 1 cut(s) 916
AjuI GAANNNNNNNTTGG 4 cut(s) 377, 395, 409, 427
AluBI AGCT 8 cut(s) 141, 168, 194, 231, 270, 685, 995, 1070
AluI AGCT 8 cut(s) 141, 168, 194, 231, 270, 685, 995, 1070
Alw21I GWGCWC 3 cut(s) 272, 378, 624
AlwI GGATC 2 cut(s) 821, 949
Ama87I CYCGRG 2 cut(s) 76, 176
Aor51HI AGCGCT 1 cut(s) 390
AoxI GGCC 8 cut(s) 39, 198, 279, 414, 532, 642, 771, 838
ApeKI GCWGC 4 cut(s) 138, 228, 355, 661
ApoI RAATTY 3 cut(s) 423, 962, 1050
AspLEI GCGC 2 cut(s) 218, 391
AspS9I GGNCC 4 cut(s) 40, 129, 415, 533
AsuC2I CCSGG 2 cut(s) 127, 418
AsuHPI GGTGA 3 cut(s) 22, 526, 923
AvaI CYCGRG 2 cut(s) 76, 176
AvaII GGWCC 1 cut(s) 129
BanI GGYRCC 3 cut(s) 326, 524, 956
BanII GRGCYC 2 cut(s) 272, 444
BauI CACGAG 1 cut(s) 265
Bbv12I GWGCWC 3 cut(s) 272, 378, 624
BbvI GCAGC 4 cut(s) 150, 240, 367, 673
BccI CCATC 3 cut(s) 298, 401, 1124
BceAI ACGGC 4 cut(s) 95, 131, 314, 872
BciT130I CCWGG 1 cut(s) 918
BciVI GTATCC 1 cut(s) 875
BclI TGATCA 1 cut(s) 745
BcnI CCSGG 2 cut(s) 127, 418
BfaI CTAG 2 cut(s) 810, 879
BfoI RGCGCY 2 cut(s) 219, 392
BfuAI ACCTGC 2 cut(s) 349, 502
BfuI GTATCC 1 cut(s) 875
BisI GCNGC 7 cut(s) 117, 139, 229, 339, 356, 581, 662
BlsI GCNGC 7 cut(s) 118, 140, 230, 340, 357, 582, 663
Bme1390I CCNGG 3 cut(s) 127, 418, 918
Bme18I GGWCC 1 cut(s) 129
BmeT110I CYCGRG 2 cut(s) 76, 176
BmgT120I GGNCC 4 cut(s) 40, 129, 415, 533
BmiI GGNNCC 3 cut(s) 328, 526, 958
BmrFI CCNGG 3 cut(s) 127, 418, 918
BmsI GCATC 2 cut(s) 128, 1124
BplI GAGNNNNNCTC 4 cut(s) 70, 102, 259, 291
BpmI CTGGAG 1 cut(s) 665
BpuMI CCSGG 2 cut(s) 127, 418
BsaBI GATNNNNATC 1 cut(s) 750
BsaHI GRCGYC 2 cut(s) 53, 336
BsaJI CCNNGG 2 cut(s) 495, 774
BsaXI ACNNNNNCTCC 2 cut(s) 1059, 1089
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 541, 542, 631, 651, 836, 1161
Bse1I ACTGG 2 cut(s) 528, 1127
Bse8I GATNNNNATC 1 cut(s) 750
BseBI CCWGG 1 cut(s) 918
BseDI CCNNGG 2 cut(s) 495, 774
BseGI GGATG 1 cut(s) 1139
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 7 cut(s) 49, 541, 542, 631, 651, 836, 1161
BseMII CTCAG 3 cut(s) 290, 291, 363
BseNI ACTGG 2 cut(s) 528, 1127
BseRI GAGGAG 2 cut(s) 247, 262
BseXI GCAGC 4 cut(s) 150, 240, 367, 673
BseYI CCCAGC 3 cut(s) 164, 437, 1137
Bsh1236I CGCG 1 cut(s) 652
BshFI GGCC 8 cut(s) 41, 200, 281, 416, 534, 644, 773, 840
BshNI GGYRCC 3 cut(s) 326, 524, 956
BsiHKAI GWGCWC 3 cut(s) 272, 378, 624
BsiHKCI CYCGRG 2 cut(s) 76, 176
BsiSI CCGG 2 cut(s) 127, 418
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 7 cut(s) 49, 541, 542, 631, 651, 836, 1161
BsmFI GGGAC 1 cut(s) 40
BsnI GGCC 8 cut(s) 41, 200, 281, 416, 534, 644, 773, 840
BsoBI CYCGRG 2 cut(s) 76, 176
Bsp1286I GDGCHC 4 cut(s) 272, 378, 444, 624
Bsp143I GATC 3 cut(s) 745, 826, 941
BspACI CCGC 5 cut(s) 117, 261, 339, 580, 652
BspANI GGCC 8 cut(s) 41, 200, 281, 416, 534, 644, 773, 840
BspCNI CTCAG 3 cut(s) 289, 292, 364
BspFNI CGCG 1 cut(s) 652
BspLI GGNNCC 3 cut(s) 328, 526, 958
BspMI ACCTGC 2 cut(s) 349, 502
BspPI GGATC 2 cut(s) 821, 949
BspT107I GGYRCC 3 cut(s) 326, 524, 956
BsrI ACTGG 2 cut(s) 528, 1127
BssECI CCNNGG 2 cut(s) 495, 774
BssMI GATC 3 cut(s) 745, 826, 941
BssNI GRCGYC 2 cut(s) 53, 336
BssSI CACGAG 1 cut(s) 265
BssT1I CCWWGG 2 cut(s) 495, 774
Bst2BI CACGAG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 918
Bst4CI ACNGT 2 cut(s) 457, 1060
BstACI GRCGYC 2 cut(s) 53, 336
BstC8I GCNNGC 3 cut(s) 7, 214, 353
BstDEI CTNAG 4 cut(s) 276, 300, 372, 686
BstENI CCTNNNNNAGG 1 cut(s) 834
BstF5I GGATG 1 cut(s) 1139
BstFNI CGCG 1 cut(s) 652
BstH2I RGCGCY 2 cut(s) 219, 392
BstHHI GCGC 2 cut(s) 218, 391
BstKTI GATC 3 cut(s) 748, 829, 944
BstMBI GATC 3 cut(s) 745, 826, 941
BstMWI GCNNNNNNNGC 8 cut(s) 116, 122, 335, 397, 531, 641, 658, 1134
BstNI CCWGG 1 cut(s) 918
BstSCI CCNGG 3 cut(s) 125, 416, 916
BstUI CGCG 1 cut(s) 652
BstV1I GCAGC 4 cut(s) 150, 240, 367, 673
BstXI CCANNNNNNTGG 1 cut(s) 208
BsuI GTATCC 1 cut(s) 875
BsuRI GGCC 8 cut(s) 41, 200, 281, 416, 534, 644, 773, 840
BtgZI GCGATG 1 cut(s) 1143
BtsCI GGATG 1 cut(s) 1139
BtsI GCAGTG 1 cut(s) 866
BtsIMutI CAGTG 4 cut(s) 66, 535, 741, 866
BveI ACCTGC 2 cut(s) 349, 502
Cac8I GCNNGC 3 cut(s) 7, 214, 353
CfoI GCGC 2 cut(s) 218, 391
Cfr13I GGNCC 4 cut(s) 40, 129, 415, 533
CseI GACGC 2 cut(s) 42, 344
Csp6I GTAC 1 cut(s) 784
CspCI CAANNNNNGTGG 2 cut(s) 949, 984
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 4 cut(s) 276, 300, 372, 686
DpnI GATC 3 cut(s) 747, 828, 943
DpnII GATC 3 cut(s) 745, 826, 941
DraIII CACNNNGTG 1 cut(s) 628
EaeI YGGCCR 1 cut(s) 198
EciI GGCGGA 1 cut(s) 250
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 2 cut(s) 495, 774
Eco147I AGGCCT 1 cut(s) 773
Eco24I GRGCYC 2 cut(s) 272, 444
Eco47I GGWCC 1 cut(s) 129
Eco47III AGCGCT 1 cut(s) 390
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 2 cut(s) 777, 1022
Eco88I CYCGRG 2 cut(s) 76, 176
EcoICRI GAGCTC 1 cut(s) 270
EcoNI CCTNNNNNAGG 1 cut(s) 834
EcoRII CCWGG 1 cut(s) 916
EcoT14I CCWWGG 2 cut(s) 495, 774
EcoT38I GRGCYC 2 cut(s) 272, 444
ErhI CCWWGG 2 cut(s) 495, 774
FaiI YATR 6 cut(s) 542, 596, 768, 836, 938, 1056
FaqI GGGAC 1 cut(s) 40
FbaI TGATCA 1 cut(s) 745
Fnu4HI GCNGC 7 cut(s) 117, 139, 229, 339, 356, 581, 662
FokI GGATG 1 cut(s) 1146
FriOI GRGCYC 2 cut(s) 272, 444
Fsp4HI GCNGC 7 cut(s) 117, 139, 229, 339, 356, 581, 662
FspBI CTAG 2 cut(s) 810, 879
GlaI GCGC 2 cut(s) 217, 390
GluI GCNGC 7 cut(s) 117, 139, 229, 339, 356, 581, 662
GsaI CCCAGC 3 cut(s) 168, 441, 1141
GsuI CTGGAG 1 cut(s) 665
HaeII RGCGCY 2 cut(s) 219, 392
HaeIII GGCC 8 cut(s) 41, 200, 281, 416, 534, 644, 773, 840
HapII CCGG 2 cut(s) 127, 418
HgaI GACGC 2 cut(s) 42, 344
HhaI GCGC 2 cut(s) 218, 391
Hin1I GRCGYC 2 cut(s) 53, 336
Hin6I GCGC 2 cut(s) 216, 389
HinP1I GCGC 2 cut(s) 216, 389
HincII GTYRAC 2 cut(s) 297, 334
HindII GTYRAC 2 cut(s) 297, 334
HinfI GANTC 7 cut(s) 71, 81, 97, 244, 368, 738, 1103
HpaI GTTAAC 1 cut(s) 297
HpaII CCGG 2 cut(s) 127, 418
HphI GGTGA 3 cut(s) 22, 526, 923
Hpy166II GTNNAC 3 cut(s) 297, 334, 548
Hpy188I TCNGA 4 cut(s) 96, 373, 674, 1002
Hpy188III TCNNGA 6 cut(s) 78, 176, 430, 755, 868, 1085
Hpy8I GTNNAC 3 cut(s) 297, 334, 548
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 7 cut(s) 142, 163, 229, 427, 697, 752, 997
HpyCH4III ACNGT 2 cut(s) 457, 1060
HpyF10VI GCNNNNNNNGC 8 cut(s) 116, 122, 335, 397, 531, 641, 658, 1134
HpyF3I CTNAG 4 cut(s) 276, 300, 372, 686
Hsp92I GRCGYC 2 cut(s) 53, 336
HspAI GCGC 2 cut(s) 216, 389
Ksp22I TGATCA 1 cut(s) 745
KspAI GTTAAC 1 cut(s) 297
Kzo9I GATC 3 cut(s) 745, 826, 941
LmnI GCTCC 4 cut(s) 275, 397, 619, 1067
Lsp1109I GCAGC 4 cut(s) 150, 240, 367, 673
LweI GCATC 2 cut(s) 128, 1124
MaeI CTAG 2 cut(s) 810, 879
MaeIII GTNAC 3 cut(s) 514, 739, 1009
MalI GATC 3 cut(s) 747, 828, 943
MboI GATC 3 cut(s) 745, 826, 941
MboII GAAGA 3 cut(s) 452, 598, 901
MfeI CAATTG 1 cut(s) 665
MhlI GDGCHC 4 cut(s) 272, 378, 444, 624
MluCI AATT 6 cut(s) 45, 250, 423, 665, 962, 1050
MlyI GAGTC 4 cut(s) 106, 238, 377, 747
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 9 cut(s) 85, 96, 268, 283, 307, 689, 700, 763, 973
MseI TTAA 3 cut(s) 296, 714, 1098
MspI CCGG 2 cut(s) 127, 418
MspR9I CCNGG 3 cut(s) 127, 418, 918
MunI CAATTG 1 cut(s) 665
MvaI CCWGG 1 cut(s) 918
MvnI CGCG 1 cut(s) 652
MwoI GCNNNNNNNGC 8 cut(s) 116, 122, 335, 397, 531, 641, 658, 1134
NciI CCSGG 2 cut(s) 127, 418
NdeII GATC 3 cut(s) 745, 826, 941
NlaIV GGNNCC 3 cut(s) 328, 526, 958
NmeAIII GCCGAG 2 cut(s) 556, 866
NmuCI GTSAC 2 cut(s) 514, 739
PaeR7I CTCGAG 1 cut(s) 76
PaqCI CACCTGC 2 cut(s) 349, 502
PceI AGGCCT 1 cut(s) 773
PfeI GAWTC 3 cut(s) 71, 81, 1103
PflMI CCANNNNNTGG 1 cut(s) 1161
PkrI GCNGC 7 cut(s) 118, 140, 230, 340, 357, 582, 663
PleI GAGTC 4 cut(s) 105, 238, 376, 746
PpsI GAGTC 4 cut(s) 105, 238, 376, 746
Psp124BI GAGCTC 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 916
PspFI CCCAGC 3 cut(s) 164, 437, 1137
PspGI CCWGG 1 cut(s) 916
PspN4I GGNNCC 3 cut(s) 328, 526, 958
PspPI GGNCC 4 cut(s) 40, 129, 415, 533
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
SacI GAGCTC 1 cut(s) 272
SaqAI TTAA 3 cut(s) 296, 714, 1098
SatI GCNGC 7 cut(s) 117, 139, 229, 339, 356, 581, 662
Sau3AI GATC 3 cut(s) 745, 826, 941
Sau96I GGNCC 4 cut(s) 40, 129, 415, 533
SchI GAGTC 4 cut(s) 106, 238, 377, 747
ScrFI CCNGG 3 cut(s) 127, 418, 918
SduI GDGCHC 4 cut(s) 272, 378, 444, 624
SfaNI GCATC 2 cut(s) 128, 1124
Sfr274I CTCGAG 1 cut(s) 76
SinI GGWCC 1 cut(s) 129
SlaI CTCGAG 1 cut(s) 76
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 6 cut(s) 45, 250, 423, 665, 962, 1050
SseBI AGGCCT 1 cut(s) 773
SsiI CCGC 5 cut(s) 117, 261, 339, 580, 652
SspMI CTAG 2 cut(s) 810, 879
SstI GAGCTC 1 cut(s) 272
StuI AGGCCT 1 cut(s) 773
StyD4I CCNGG 3 cut(s) 125, 416, 916
StyI CCWWGG 2 cut(s) 495, 774
TaaI ACNGT 2 cut(s) 457, 1060
TaqI TCGA 2 cut(s) 77, 444
TasI AATT 6 cut(s) 45, 250, 423, 665, 962, 1050
TauI GCSGC 3 cut(s) 119, 341, 583
TfiI GAWTC 3 cut(s) 71, 81, 1103
Tru1I TTAA 3 cut(s) 296, 714, 1098
Tru9I TTAA 3 cut(s) 296, 714, 1098
TscAI CASTG 4 cut(s) 73, 535, 748, 866
TseFI GTSAC 2 cut(s) 514, 739
TseI GCWGC 4 cut(s) 138, 228, 355, 661
Tsp45I GTSAC 2 cut(s) 514, 739
TspDTI ATGAA 6 cut(s) 398, 469, 611, 864, 1043, 1062
TspRI CASTG 4 cut(s) 73, 535, 748, 866
Van91I CCANNNNNTGG 1 cut(s) 1161
VpaK11BI GGWCC 1 cut(s) 129
XagI CCTNNNNNAGG 1 cut(s) 834
XapI RAATTY 3 cut(s) 423, 962, 1050
XhoI CTCGAG 1 cut(s) 76
XspI CTAG 2 cut(s) 810, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.