pycom15g04170

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005994
Physical Location & Seq
Forward (+)
2517186 .. 2518054
869 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 336 bp
ATGTGGGCTGGAACTTGGAGAGTTGGAGGTGTTCTCGCTGTTTCTCGTGCATTTGGTGATAGGCTGTTGAAGCAGTACGTTGTTGCTGATCCAGAAATCCAGGAGGAAAAGATTGACAACTCTCTTGAGTTCCTTATCCTTGCTAGTGATGGACTGTGGGATGTTGTCACCAATGAGGAGGCCGTAGCAATGATCAAACCTATTCAGGATCCAAAGCAGGCAGCGGAGAGGTTAATGCAGGAAGCATATCAGAGGGGGAGTGCAGATAACATCACGTGTGTTGTTGTTCGCTTCCTGGCTAACCAAGGGGGCTCCTCACGAAGCAGTTCCGGCTAA

Protein Analysis

112

Amino Acids

12.17

Weight (kDa)

4.73

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 3 - 91 2.8e-28 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 224
AclWI GGATC 3 cut(s) 83, 203, 216
AcvI CACGTG 1 cut(s) 276
AfaI GTAC 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 275
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 2 cut(s) 99, 294
AlwI GGATC 3 cut(s) 83, 203, 216
AoxI GGCC 1 cut(s) 180
ApeKI GCWGC 1 cut(s) 221
Asp700I GAANNNNTTC 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 68, 160
BamHI GGATCC 1 cut(s) 208
BanII GRGCYC 1 cut(s) 314
BauI CACGAG 1 cut(s) 45
BbrPI CACGTG 1 cut(s) 276
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 143
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 2 cut(s) 101, 296
BclI TGATCA 1 cut(s) 192
BfaI CTAG 1 cut(s) 144
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 2 cut(s) 101, 296
BmiI GGNNCC 2 cut(s) 210, 313
BmrFI CCNGG 2 cut(s) 101, 296
BplI GAGNNNNNCTC 2 cut(s) 18, 50
BpuEI CTTGAG 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 195
BseBI CCWGG 2 cut(s) 101, 296
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 166
BseMI GCAATG 1 cut(s) 195
BseRI GAGGAG 2 cut(s) 191, 304
BseXI GCAGC 1 cut(s) 233
BsgI GTGCAG 1 cut(s) 282
BshFI GGCC 1 cut(s) 182
BsiSI CCGG 1 cut(s) 330
BsnI GGCC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 3 cut(s) 88, 192, 208
BspACI CCGC 1 cut(s) 224
BspANI GGCC 1 cut(s) 182
BspLI GGNNCC 2 cut(s) 210, 313
BspPI GGATC 3 cut(s) 83, 203, 216
BsrDI GCAATG 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 3 cut(s) 88, 192, 208
BssSI CACGAG 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 304
Bst2BI CACGAG 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 101, 296
Bst4CI ACNGT 1 cut(s) 156
BstBAI YACGTR 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 219
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 3 cut(s) 91, 195, 211
BstMBI GATC 3 cut(s) 88, 192, 208
BstMWI GCNNNNNNNGC 2 cut(s) 70, 330
BstNI CCWGG 2 cut(s) 101, 296
BstSCI CCNGG 2 cut(s) 99, 294
BstV1I GCAGC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 1 cut(s) 182
BtsCI GGATG 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 219
Csp6I GTAC 1 cut(s) 76
CviJI RGCY 6 cut(s) 8, 64, 182, 299, 312, 333
CviKI_1 RGCY 6 cut(s) 8, 64, 182, 299, 312, 333
CviQI GTAC 1 cut(s) 76
DpnI GATC 3 cut(s) 90, 194, 210
DpnII GATC 3 cut(s) 88, 192, 208
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 314
Eco72I CACGTG 1 cut(s) 276
EcoRII CCWGG 2 cut(s) 99, 294
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 304
FaiI YATR 1 cut(s) 247
FbaI TGATCA 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 1 cut(s) 173
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 1 cut(s) 144
GluI GCNGC 1 cut(s) 222
HaeIII GGCC 1 cut(s) 182
HapII CCGG 1 cut(s) 330
HpaII CCGG 1 cut(s) 330
HphI GGTGA 2 cut(s) 68, 160
Hpy188I TCNGA 1 cut(s) 252
Hpy188III TCNNGA 4 cut(s) 92, 125, 206, 318
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4IV ACGT 2 cut(s) 78, 275
HpyCH4V TGCA 3 cut(s) 50, 238, 263
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 330
HpySE526I ACGT 2 cut(s) 78, 275
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 3 cut(s) 88, 192, 208
LmnI GCTCC 1 cut(s) 317
LpnPI CCDG 8 cut(s) 86, 105, 113, 191, 203, 224, 281, 308
Lsp1109I GCAGC 1 cut(s) 233
MaeI CTAG 1 cut(s) 144
MaeII ACGT 2 cut(s) 78, 275
MaeIII GTNAC 1 cut(s) 166
MalI GATC 3 cut(s) 90, 194, 210
MboI GATC 3 cut(s) 88, 192, 208
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 4
MnlI CCTC 7 cut(s) 20, 97, 169, 172, 222, 246, 325
MroXI GAANNNNTTC 1 cut(s) 325
MseI TTAA 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 2 cut(s) 101, 296
MvaI CCWGG 2 cut(s) 101, 296
MwoI GCNNNNNNNGC 2 cut(s) 70, 330
NdeII GATC 3 cut(s) 88, 192, 208
NlaIV GGNNCC 2 cut(s) 210, 313
NmuCI GTSAC 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 325
PfoI TCCNGGA 1 cut(s) 99
PkrI GCNGC 1 cut(s) 223
PmaCI CACGTG 1 cut(s) 276
PmlI CACGTG 1 cut(s) 276
Ppu21I YACGTR 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 99, 294
PspCI CACGTG 1 cut(s) 276
PspGI CCWGG 2 cut(s) 99, 294
PspN4I GGNNCC 2 cut(s) 210, 313
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 3 cut(s) 88, 192, 208
ScrFI CCNGG 2 cut(s) 101, 296
SduI GDGCHC 1 cut(s) 314
SetI ASST 5 cut(s) 31, 81, 202, 233, 278
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SsiI CCGC 1 cut(s) 224
SspMI CTAG 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 99, 294
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 1 cut(s) 156
TaiI ACGT 2 cut(s) 81, 278
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 325
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.