pycom15g06190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013622
Physical Location & Seq
Reverse (-)
3810127 .. 3811613
1487 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGGCTTCCACCCCCAAGCCAGTAAAACTTGCAGATCTCCTCCAAGAGCCGCAAGAACCCTTCGTCCTAGAGGTTTACCTATCCGAAAGGGGTTACCTGAGAAAAAGCTCGAGTACAAACACCAATTCGAGGAGTAGGAATAGCAATTCGAGCAAGAAACTTACCAGTTCATCCAGTTGGGGCAATCTCAGCTGCCCTAAGATACTGAGATCTCTGTATAAAAAGCTTGTTTCAAGGCATAGTGAGACAACAAGCATTAAGGGTTGTGAAAAGAGAGAGGGGGAGTCCAATGCCGGCACCAACACGCAGAGAAGTAACCGGGAGAGCGGGGAATCGGATAGAATTTCATCTGCTAGTAGTAGGACGCAGTATGATTCGTGCACCGAGGGCGAAAAAGAATCAACAGATGCAGCCCCTGTTTCGTTGCGAAATGATCATGATGCCTCAGTTGCAGCGGACGCTTCTCAAGTTTCGGAATCCTGCAATATGAAAGACAGAAAGATAGAACGCCTCAACGGACAATCATCGGAGATTCACCGTAAGCAACAAAACTCCGTTTCGGTACCAGAAGATAAACCTCCCCATGCAAATGCAAGTTTACCTCTTCATAACAAATATGAACTGCAAACTCCATGCCCATCCACATATAGCTTCAACAAGATTACAGAAGACTCAATATTATCATCTTCTCTCTGGCAACTACTTTTCCACCCACCATTTGAGAACCCAACAGCTTCTGGAGTTTCAGAAATGCTAGAGCCTGCCCGGTCTAGCATCAATCCATCCCCTCATTTTGCAAAATCGAAGAGGGTGTTGCAGCAGACAAGGCAGCTCCTGTTTGATTGCGTGAGGGAGATGACGGAAAATCATGCAAAGAAAATGAAGGATCACCAGCAGCATAACTGCGACCAATTTCTTGGAGCCGAAGAGATTGGGAAGCTGGTTTATGAGAAGTTGGGGAGTTGGGGGAGCAAACAGGCTGGGCACCAAGCCAACGTCAACTTTTTGTTGGAGTCGGACTTGTTGGCTTCGGCTGAAGAATGGGGTGATAATTACCAAGAGAGAAGGGAAATTGGGTCGGAAATTGGAGACGCCATTTTACAAGACATCATCAAAGACATATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

376

Amino Acids

41.88

Weight (kDa)

6.29

Isoelectric Point (pI)

63.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 243 - 372 5.6e-08 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32690
malus_domestica MD08G1078600.v1.1 MD15G1065400.v1.1
prunus_persica Prupe.1G419500_v2.0.a1
pyrus_communis pycom08g06500 pycom15g06190
rosa_chinensis RchiOBHm_Chr6g0309981
rosa_laevigata RLG00000010519
rosa_multiflora Rmu_sc0000294.1_g000010
rosa_roxburghii Rroxscaffold_7G00158980
rosa_rugosa Rorug06G0376500 Rorug06G0376600
rosa_samantha Rh6AG490300 Rh6BG499800 Rh6CG504600 Rh6CG504700 Rh6DG490700
rosa_wichuraiana Rw6G042680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 562
AccB1I GGYRCC 3 cut(s) 296, 562, 984
AccBSI CCGCTC 1 cut(s) 327
AciI CCGC 3 cut(s) 50, 327, 455
AclWI GGATC 1 cut(s) 894
AcsI RAATTY 1 cut(s) 342
AcuI CTGAAG 1 cut(s) 1056
AcyI GRCGYC 1 cut(s) 1092
AfaI GTAC 2 cut(s) 115, 564
AfiI CCNNNNNNNGG 1 cut(s) 129
AgsI TTSAA 2 cut(s) 234, 655
AloI GAACNNNNNNTCC 2 cut(s) 48, 80
AluBI AGCT 7 cut(s) 108, 192, 226, 651, 734, 832, 940
AluI AGCT 7 cut(s) 108, 192, 226, 651, 734, 832, 940
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 2 cut(s) 239, 1083
Alw44I GTGCAC 1 cut(s) 379
AlwI GGATC 1 cut(s) 894
AlwNI CAGNNNCTG 3 cut(s) 416, 737, 835
Ama87I CYCGRG 1 cut(s) 109
ApaLI GTGCAC 1 cut(s) 379
ApeKI GCWGC 6 cut(s) 192, 410, 452, 817, 829, 895
ApoI RAATTY 1 cut(s) 342
Asp718I GGTACC 1 cut(s) 562
AsuC2I CCSGG 2 cut(s) 320, 766
AsuHPI GGTGA 3 cut(s) 527, 881, 1058
AvaI CYCGRG 1 cut(s) 109
BaeGI GKGCMC 2 cut(s) 383, 987
BanI GGYRCC 3 cut(s) 296, 562, 984
BbsI GAAGAC 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 6 cut(s) 179, 422, 464, 829, 841, 907
BccI CCATC 2 cut(s) 646, 790
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 2 cut(s) 320, 766
BcoDI GTCTC 2 cut(s) 239, 1083
BfaI CTAG 4 cut(s) 68, 354, 755, 771
BglII AGATCT 2 cut(s) 34, 209
BisI GCNGC 7 cut(s) 50, 193, 411, 453, 818, 830, 896
BlsI GCNGC 7 cut(s) 51, 194, 412, 454, 819, 831, 897
Bme1390I CCNGG 2 cut(s) 320, 766
BmeT110I CYCGRG 1 cut(s) 109
BmiI GGNNCC 4 cut(s) 298, 564, 922, 986
BmrFI CCNGG 2 cut(s) 320, 766
BmsI GCATC 3 cut(s) 397, 430, 783
BpiI GAAGAC 1 cut(s) 675
BpmI CTGGAG 1 cut(s) 759
BpuEI CTTGAG 1 cut(s) 450
BpuMI CCSGG 2 cut(s) 320, 766
BsaHI GRCGYC 1 cut(s) 1092
BsaJI CCNNGG 1 cut(s) 384
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 3 cut(s) 20, 165, 174
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 3 cut(s) 170, 638, 782
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMII CTCAG 4 cut(s) 89, 197, 202, 459
BseNI ACTGG 3 cut(s) 20, 165, 174
BseRI GAGGAG 2 cut(s) 29, 145
BseSI GKGCMC 2 cut(s) 383, 987
BseXI GCAGC 6 cut(s) 179, 422, 464, 829, 841, 907
BseYI CCCAGC 1 cut(s) 980
BshNI GGYRCC 3 cut(s) 296, 562, 984
BsiHKAI GWGCWC 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 3 cut(s) 294, 319, 766
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 2 cut(s) 239, 1083
BsmBI CGTCTC 1 cut(s) 1083
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 2 cut(s) 383, 987
Bsp143I GATC 4 cut(s) 34, 209, 433, 886
BspACI CCGC 3 cut(s) 50, 327, 455
BspCNI CTCAG 4 cut(s) 90, 198, 201, 458
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 4 cut(s) 298, 564, 922, 986
BspPI GGATC 1 cut(s) 894
BspT107I GGYRCC 3 cut(s) 296, 562, 984
BsrBI CCGCTC 1 cut(s) 327
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 3 cut(s) 20, 165, 174
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 4 cut(s) 34, 209, 433, 886
BssNI GRCGYC 1 cut(s) 1092
Bst4CI ACNGT 1 cut(s) 539
Bst6I CTCTTC 3 cut(s) 609, 800, 921
BstACI GRCGYC 1 cut(s) 1092
BstC8I GCNNGC 2 cut(s) 295, 762
BstDEI CTNAG 5 cut(s) 98, 188, 198, 206, 445
BstEII GGTNACC 1 cut(s) 92
BstF5I GGATG 3 cut(s) 170, 638, 782
BstKTI GATC 4 cut(s) 37, 212, 436, 889
BstMAI GTCTC 2 cut(s) 239, 1083
BstMBI GATC 4 cut(s) 34, 209, 433, 886
BstMWI GCNNNNNNNGC 6 cut(s) 150, 189, 387, 449, 458, 826
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 318, 764
BstSLI GKGCMC 2 cut(s) 383, 987
BstV1I GCAGC 6 cut(s) 179, 422, 464, 829, 841, 907
BstV2I GAAGAC 1 cut(s) 675
BstX2I RGATCY 2 cut(s) 34, 209
BstXI CCANNNNNNTGG 1 cut(s) 917
BstYI RGATCY 2 cut(s) 34, 209
BtsCI GGATG 3 cut(s) 170, 638, 782
Cac8I GCNNGC 2 cut(s) 295, 762
CaiI CAGNNNCTG 3 cut(s) 416, 737, 835
CciI TCATGA 1 cut(s) 436
Cfr10I RCCGGY 1 cut(s) 293
CseI GACGC 3 cut(s) 373, 467, 1100
Csp6I GTAC 2 cut(s) 114, 563
CviAII CATG 4 cut(s) 437, 584, 633, 869
CviQI GTAC 2 cut(s) 114, 563
DdeI CTNAG 5 cut(s) 98, 188, 198, 206, 445
DpnI GATC 4 cut(s) 36, 211, 435, 888
DpnII GATC 4 cut(s) 34, 209, 433, 886
Eam1104I CTCTTC 3 cut(s) 609, 800, 921
EarI CTCTTC 3 cut(s) 609, 800, 921
Eco57I CTGAAG 1 cut(s) 1056
Eco88I CYCGRG 1 cut(s) 109
Eco91I GGTNACC 1 cut(s) 92
EcoO65I GGTNACC 1 cut(s) 92
Esp3I CGTCTC 1 cut(s) 1083
FaeI CATG 4 cut(s) 440, 587, 636, 872
FatI CATG 4 cut(s) 436, 583, 632, 868
FauI CCCGC 1 cut(s) 320
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 7 cut(s) 50, 193, 411, 453, 818, 830, 896
FokI GGATG 3 cut(s) 157, 625, 769
Fsp4HI GCNGC 7 cut(s) 50, 193, 411, 453, 818, 830, 896
FspBI CTAG 4 cut(s) 68, 354, 755, 771
GluI GCNGC 7 cut(s) 50, 193, 411, 453, 818, 830, 896
GsaI CCCAGC 1 cut(s) 984
GsuI CTGGAG 1 cut(s) 759
HapII CCGG 3 cut(s) 294, 319, 766
HgaI GACGC 3 cut(s) 373, 467, 1100
Hin1I GRCGYC 1 cut(s) 1092
Hin1II CATG 4 cut(s) 440, 587, 636, 872
HincII GTYRAC 1 cut(s) 1000
HindII GTYRAC 1 cut(s) 1000
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 8 cut(s) 284, 332, 374, 398, 476, 532, 671, 1013
HpaII CCGG 3 cut(s) 294, 319, 766
HphI GGTGA 3 cut(s) 527, 881, 1058
Hpy166II GTNNAC 4 cut(s) 76, 381, 599, 1000
Hpy188I TCNGA 8 cut(s) 85, 337, 475, 529, 748, 1018, 1081, 1127
Hpy188III TCNNGA 2 cut(s) 437, 738
Hpy8I GTNNAC 4 cut(s) 76, 381, 599, 1000
HpyAV CCTTC 3 cut(s) 70, 877, 1059
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 1 cut(s) 996
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 189, 387, 449, 458, 826
HpyF3I CTNAG 5 cut(s) 98, 188, 198, 206, 445
HpySE526I ACGT 1 cut(s) 996
Hsp92I GRCGYC 1 cut(s) 1092
Hsp92II CATG 4 cut(s) 440, 587, 636, 872
KpnI GGTACC 1 cut(s) 566
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 4 cut(s) 34, 209, 433, 886
LmnI GCTCC 3 cut(s) 837, 920, 969
Lsp1109I GCAGC 6 cut(s) 179, 422, 464, 829, 841, 907
LweI GCATC 3 cut(s) 397, 430, 783
MaeI CTAG 4 cut(s) 68, 354, 755, 771
MaeII ACGT 1 cut(s) 996
MaeIII GTNAC 2 cut(s) 92, 314
MalI GATC 4 cut(s) 36, 211, 435, 888
MbiI CCGCTC 1 cut(s) 327
MboI GATC 4 cut(s) 34, 209, 433, 886
MboII GAAGA 7 cut(s) 581, 596, 678, 680, 817, 938, 1049
MflI RGATCY 2 cut(s) 34, 209
MhlI GDGCHC 2 cut(s) 383, 987
MluCI AATT 7 cut(s) 124, 145, 342, 911, 1051, 1071, 1083
MlyI GAGTC 3 cut(s) 293, 665, 1022
MmeI TCCRAC 3 cut(s) 990, 996, 1059
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 588
MspA1I CMGCKG 2 cut(s) 192, 455
MspI CCGG 3 cut(s) 294, 319, 766
MspR9I CCNGG 2 cut(s) 320, 766
MwoI GCNNNNNNNGC 6 cut(s) 150, 189, 387, 449, 458, 826
NaeI GCCGGC 1 cut(s) 295
NciI CCSGG 2 cut(s) 320, 766
NdeII GATC 4 cut(s) 34, 209, 433, 886
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 4 cut(s) 440, 587, 636, 872
NlaIV GGNNCC 4 cut(s) 298, 564, 922, 986
PaeR7I CTCGAG 1 cut(s) 109
PagI TCATGA 1 cut(s) 436
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 5 cut(s) 332, 374, 398, 476, 532
PkrI GCNGC 7 cut(s) 51, 194, 412, 454, 819, 831, 897
PleI GAGTC 3 cut(s) 292, 665, 1021
PpsI GAGTC 3 cut(s) 292, 665, 1021
PspEI GGTNACC 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 980
PspN4I GGNNCC 4 cut(s) 298, 564, 922, 986
PspXI VCTCGAGB 1 cut(s) 109
PstNI CAGNNNCTG 3 cut(s) 416, 737, 835
PsuI RGATCY 2 cut(s) 34, 209
PvuII CAGCTG 1 cut(s) 192
RsaI GTAC 2 cut(s) 115, 564
RsaNI GTAC 2 cut(s) 114, 563
RseI CAYNNNNRTG 1 cut(s) 588
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 7 cut(s) 50, 193, 411, 453, 818, 830, 896
Sau3AI GATC 4 cut(s) 34, 209, 433, 886
SchI GAGTC 3 cut(s) 293, 665, 1022
ScrFI CCNGG 2 cut(s) 320, 766
SduI GDGCHC 2 cut(s) 383, 987
SfaNI GCATC 3 cut(s) 397, 430, 783
Sfr274I CTCGAG 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 2 cut(s) 109, 465
SmoI CTYRAG 2 cut(s) 109, 465
Sse9I AATT 7 cut(s) 124, 145, 342, 911, 1051, 1071, 1083
SsiI CCGC 3 cut(s) 50, 327, 455
SspI AATATT 1 cut(s) 678
SspMI CTAG 4 cut(s) 68, 354, 755, 771
StyD4I CCNGG 2 cut(s) 318, 764
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 1 cut(s) 999
TaqI TCGA 4 cut(s) 110, 128, 149, 803
TasI AATT 7 cut(s) 124, 145, 342, 911, 1051, 1071, 1083
TatI WGTACW 1 cut(s) 113
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 5 cut(s) 332, 374, 398, 476, 532
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseI GCWGC 6 cut(s) 192, 410, 452, 817, 829, 895
TspDTI ATGAA 6 cut(s) 159, 336, 503, 596, 633, 896
TspGWI ACGGA 3 cut(s) 531, 544, 875
VneI GTGCAC 1 cut(s) 379
XapI RAATTY 1 cut(s) 342
XhoI CTCGAG 1 cut(s) 109
XspI CTAG 4 cut(s) 68, 354, 755, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.