pycom15g06620

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003645
Physical Location & Seq
Reverse (-)
4060952 .. 4062872
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 834 bp
ATGTCGACCAGAGGCCCTGCCCTCTTCTCCGCCATTGGCAAGAAAGCCACAGACTTGCTTAACAAGGACTACAACACTGACCAGAAGATAAACATTACCACCTACACTGAAACTGGACTTGCCCTCAACTCGGGTTTGGCTAATAAGGGAGGTCTGTCTTCTGGGGATATGGCAGCACAATACAATTACAAGAATGTCGCGCTCGATTTCAAAGCTGATACACAATCAAATGTCTCGACAGTGCTCGCTGTCACTGACATCTTGCCTTCTACCAAAACCATCGCTTCAATCAAACTCCCTGATTACAAATCTGGCAAGTTGGAAGTCCAGTATCTTCATGAACATGCATCCTTCACAACCACTGTTGGTCTGAACGAGTCCCCTGCTGTGGACTTTTCGGCAACCATTGGTACCCCGGCCATTGCCTTTGGAGCAGAAGCTAGTTTCTTGACAGGTTCCAGAGTGTTTTCTAAGTACAATGCCGGTGTCAGCTTTACACAGCACGATTCAACAGCTTCTGTGATTCTGGCTGACAAAGGAGATTCACTACGGGCGTCATACGTGCATCAACTGAGCAGGCTGAATGGGGGAGCTGTAGTTGGAGAGGTGAACAGAAAGTTCTCCACGAACGAGAACATGCTAACAGTCGGAGGTTCATACGTGGTTGACTCTGAGACGGTTGTGAAAGCGAGGCTCAACAACCACGGCAATCTTGGGGCTCTTTTACAGCACCAGCTCACACCCAAATCTTCACTTACACTCTCTGCAGCCTTCGACACCAAGGCATTAGAGAGGAATCCGAACTTGGGGTTGGCGCTCTCCCTCAAGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

278

Amino Acids

29.31

Weight (kDa)

8.6

Isoelectric Point (pI)

20.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 5 - 268 4.1e-67 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 1 cut(s) 410
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 200
AciI CCGC 1 cut(s) 30
AcoI YGGCCR 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 554
AfaI GTAC 2 cut(s) 412, 476
AfiI CCNNNNNNNGG 3 cut(s) 130, 388, 806
AgsI TTSAA 3 cut(s) 211, 288, 510
AjuI GAANNNNNNNTTGG 4 cut(s) 788, 794, 820, 826
AluBI AGCT 6 cut(s) 215, 440, 492, 515, 593, 736
AluI AGCT 6 cut(s) 215, 440, 492, 515, 593, 736
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 238, 668
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 2 cut(s) 13, 417
ApeKI GCWGC 2 cut(s) 173, 767
Asp718I GGTACC 1 cut(s) 410
AspLEI GCGC 2 cut(s) 202, 817
AspS9I GGNCC 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 1 cut(s) 619
AvaI CYCGRG 1 cut(s) 130
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 1 cut(s) 721
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 2 cut(s) 185, 779
BccI CCATC 1 cut(s) 287
BceAI ACGGC 1 cut(s) 721
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 2 cut(s) 238, 668
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 2 cut(s) 594, 765
BfoI RGCGCY 1 cut(s) 818
BisI GCNGC 2 cut(s) 174, 768
BlsI GCNGC 2 cut(s) 175, 769
Bme1390I CCNGG 1 cut(s) 416
BmeT110I CYCGRG 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 2 cut(s) 412, 457
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 2 cut(s) 356, 574
BpiI GAAGAC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 809
BpuMI CCSGG 1 cut(s) 416
BsaAI YACGTR 2 cut(s) 562, 661
BsaHI GRCGYC 1 cut(s) 554
BsaJI CCNNGG 3 cut(s) 414, 703, 780
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 388, 806
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 2 cut(s) 118, 328
Bse3DI GCAATG 1 cut(s) 420
BseDI CCNNGG 3 cut(s) 414, 703, 780
BseGI GGATG 1 cut(s) 347
BseLI CCNNNNNNNGG 3 cut(s) 130, 388, 806
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 563, 663
BseNI ACTGG 2 cut(s) 118, 328
BseXI GCAGC 2 cut(s) 185, 779
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 2 cut(s) 15, 419
BshNI GGYRCC 1 cut(s) 410
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 2 cut(s) 416, 483
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 3 cut(s) 130, 388, 806
BsmAI GTCTC 2 cut(s) 238, 668
BsmBI CGTCTC 1 cut(s) 668
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 2 cut(s) 15, 419
BsoBI CYCGRG 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 246, 721
BspACI CCGC 1 cut(s) 30
BspANI GGCC 2 cut(s) 15, 419
BspCNI CTCAG 2 cut(s) 564, 664
BspFNI CGCG 1 cut(s) 200
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 412, 457
BspMAI CTGCAG 1 cut(s) 769
BspT107I GGYRCC 1 cut(s) 410
BsrDI GCAATG 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 482
BsrI ACTGG 2 cut(s) 118, 328
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 3 cut(s) 414, 703, 780
BssNI GRCGYC 1 cut(s) 554
BssT1I CCWWGG 1 cut(s) 780
Bst4CI ACNGT 4 cut(s) 241, 364, 646, 679
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 1 cut(s) 554
BstBAI YACGTR 2 cut(s) 562, 661
BstC8I GCNNGC 2 cut(s) 246, 578
BstDEI CTNAG 3 cut(s) 471, 572, 672
BstDSI CCRYGG 1 cut(s) 703
BstF5I GGATG 1 cut(s) 347
BstFNI CGCG 1 cut(s) 200
BstH2I RGCGCY 1 cut(s) 818
BstHHI GCGC 2 cut(s) 202, 817
BstMAI GTCTC 2 cut(s) 238, 668
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNSI RCATGY 2 cut(s) 347, 640
BstSCI CCNGG 1 cut(s) 414
BstSFI CTRYAG 2 cut(s) 594, 765
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 2 cut(s) 185, 779
BstV2I GAAGAC 1 cut(s) 150
BsuRI GGCC 2 cut(s) 15, 419
BtgI CCRYGG 1 cut(s) 703
BtgZI GCGATG 1 cut(s) 265
BtsCI GGATG 1 cut(s) 347
BtsIMutI CAGTG 5 cut(s) 75, 105, 246, 252, 360
Cac8I GCNNGC 2 cut(s) 246, 578
CaiI CAGNNNCTG 1 cut(s) 518
CciI TCATGA 1 cut(s) 337
CfoI GCGC 2 cut(s) 202, 817
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 14
CseI GACGC 1 cut(s) 543
Csp6I GTAC 2 cut(s) 411, 475
CviAII CATG 3 cut(s) 338, 344, 637
CviQI GTAC 2 cut(s) 411, 475
DdeI CTNAG 3 cut(s) 471, 572, 672
EaeI YGGCCR 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 780
Eco24I GRGCYC 1 cut(s) 721
Eco88I CYCGRG 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 14
EcoT14I CCWWGG 1 cut(s) 780
EcoT22I ATGCAT 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 1 cut(s) 780
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 3 cut(s) 341, 347, 640
FaiI YATR 6 cut(s) 170, 339, 345, 559, 638, 658
FaqI GGGAC 1 cut(s) 364
FatI CATG 3 cut(s) 337, 343, 636
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 174, 768
FokI GGATG 1 cut(s) 334
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 2 cut(s) 174, 768
FspBI CTAG 1 cut(s) 441
GlaI GCGC 2 cut(s) 201, 816
GluI GCNGC 2 cut(s) 174, 768
HaeII RGCGCY 1 cut(s) 818
HaeIII GGCC 2 cut(s) 15, 419
HapII CCGG 2 cut(s) 416, 483
HgaI GACGC 1 cut(s) 543
HhaI GCGC 2 cut(s) 202, 817
Hin1I GRCGYC 1 cut(s) 554
Hin1II CATG 3 cut(s) 341, 347, 640
Hin6I GCGC 2 cut(s) 200, 815
HinP1I GCGC 2 cut(s) 200, 815
HincII GTYRAC 2 cut(s) 6, 667
HindII GTYRAC 2 cut(s) 6, 667
HinfI GANTC 6 cut(s) 377, 506, 523, 542, 668, 796
HpaII CCGG 2 cut(s) 416, 483
HphI GGTGA 1 cut(s) 619
Hpy166II GTNNAC 4 cut(s) 6, 391, 610, 667
Hpy188I TCNGA 4 cut(s) 372, 650, 673, 801
Hpy188III TCNNGA 4 cut(s) 235, 338, 448, 459
Hpy8I GTNNAC 4 cut(s) 6, 391, 610, 667
HpyAV CCTTC 3 cut(s) 276, 361, 781
HpyCH4III ACNGT 4 cut(s) 241, 364, 646, 679
HpyCH4IV ACGT 2 cut(s) 561, 660
HpyCH4V TGCA 3 cut(s) 347, 565, 767
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 3 cut(s) 471, 572, 672
HpySE526I ACGT 2 cut(s) 561, 660
Hsp92I GRCGYC 1 cut(s) 554
Hsp92II CATG 3 cut(s) 341, 347, 640
HspAI GCGC 2 cut(s) 200, 815
KpnI GGTACC 1 cut(s) 414
LmnI GCTCC 2 cut(s) 431, 590
Lsp1109I GCAGC 2 cut(s) 185, 779
LweI GCATC 2 cut(s) 356, 574
MaeI CTAG 1 cut(s) 441
MaeII ACGT 2 cut(s) 561, 660
MaeIII GTNAC 1 cut(s) 250
MboII GAAGA 5 cut(s) 16, 97, 150, 326, 741
MhlI GDGCHC 2 cut(s) 246, 721
MluCI AATT 1 cut(s) 184
MlyI GAGTC 2 cut(s) 386, 662
MmeI TCCRAC 3 cut(s) 300, 580, 628
MnlI CCTC 9 cut(s) 5, 32, 134, 143, 598, 644, 684, 786, 833
Mph1103I ATGCAT 1 cut(s) 349
MseI TTAA 1 cut(s) 60
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 2 cut(s) 416, 483
MspR9I CCNGG 1 cut(s) 416
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 431
NciI CCSGG 1 cut(s) 416
NlaIII CATG 3 cut(s) 341, 347, 640
NlaIV GGNNCC 2 cut(s) 412, 457
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 349
NspI RCATGY 2 cut(s) 347, 640
PagI TCATGA 1 cut(s) 337
PfeI GAWTC 4 cut(s) 506, 523, 542, 796
PkrI GCNGC 2 cut(s) 175, 769
PleI GAGTC 2 cut(s) 385, 662
PpsI GAGTC 2 cut(s) 385, 662
Ppu21I YACGTR 2 cut(s) 562, 661
PspN4I GGNNCC 2 cut(s) 412, 457
PspPI GGNCC 1 cut(s) 14
PstI CTGCAG 1 cut(s) 769
PstNI CAGNNNCTG 1 cut(s) 518
RsaI GTAC 2 cut(s) 412, 476
RsaNI GTAC 2 cut(s) 411, 475
RseI CAYNNNNRTG 1 cut(s) 342
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 60
SatI GCNGC 2 cut(s) 174, 768
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 2 cut(s) 386, 662
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 2 cut(s) 246, 721
SfaNI GCATC 2 cut(s) 356, 574
SfcI CTRYAG 2 cut(s) 594, 765
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 1 cut(s) 824
SmoI CTYRAG 1 cut(s) 824
Sse9I AATT 1 cut(s) 184
SsiI CCGC 1 cut(s) 30
SspMI CTAG 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 4 cut(s) 241, 364, 646, 679
TaiI ACGT 2 cut(s) 564, 663
TaqI TCGA 4 cut(s) 5, 204, 236, 774
TasI AATT 1 cut(s) 184
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 4 cut(s) 506, 523, 542, 796
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TscAI CASTG 5 cut(s) 82, 112, 246, 259, 367
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 173, 767
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 3 cut(s) 326, 354, 645
TspRI CASTG 5 cut(s) 82, 112, 246, 259, 367
XceI RCATGY 2 cut(s) 347, 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 441
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.