pycom15g07300
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009047
Physical Location & Seq
Reverse (-)
4463383 .. 4464912
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1002 bp
ATGGCCCTGGAACTAATGATGGGATTGATTGACAGTTTTGCAGCGAAGAGGGAGGAGAATGCCGTGAAGGAGGCGGCCTCCGCCGGAATCCAGAGCGTCGAGAAGTTTATCAGCTTGATTTCGCAACACCATCACCAGTCGGTGTCGTCGAACACGGAAGATGAAACGGAGTATAAAGAGGTGGCGGACATGGCAGTGACCAAGTTCAGGAAGGTCATTTCGTTGTTGGACAAAGGAAGAATCGGCCACGCTCGGTTCCGGAAAGCTCCGGTAACCCAAATTCCGACACCTTCAATTTCAGAACCCCATGATCAAGAAACAGAGCAACCTTCTGTTTTCAGAGCAGAACAATCTTCTGCTTTCAAGGTTTACTGTCCGACGCCGGTTGTTCGCCTTCCGCCCCTGCCGCAAAATCCTCATCTAAAAACTACTCCGGTTGCGCTGAAAAAAGGTATTGGGTGCGCTGAGAGAAAGATGGATTTTGCTGCTGCTGCTACCATCAGCTTCTCGCCTTCGGCACCCAAAACTGCCGCGAATTCATTCATTTCTTCTTTAACTGCTGGGGATGCTGCTGAGCAATCGATCTCTTCTGGGTTTCAATTCACAAACATGTCGCAGTCGTCTTCCGGTAGGCCGCCGCTGTCTTCGTCGTCGTTGAAGAGGAAGTGCAGCTCCGTCGACAATGTTTCTCGTCTCCGGTGCGGCTCTACTACTGGGCGTTGCCACTGTTCGAAGACAAGGGTTCCTGCAATTAGTATGAAATTGGCTGATATTCCACCTGATGATTATTCTTGGAGAAAGTACGGTCAAAAACCCATCAAGGGTTCCCCTTATCCAAGAGGGTATTACAAGTGTAGCAGCGAGAGAGGTTGCCTTGCACGCAAGCACGTGGAGCGCGCTGTAGACGATCCCACCATGCTGATCGTGACCTACGAAGGCGATCACAATCACTCCCTCAGCGTCACCGATCCAACGGCCACCCTCATCCTCGAATCGTCTTGA

Protein Analysis

334

Amino Acids

36.29

Weight (kDa)

9.02

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 261 - 317 1.2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 517
AccI GTMKAC 2 cut(s) 678, 903
AccII CGCG 2 cut(s) 533, 897
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 9 cut(s) 74, 81, 185, 398, 407, 531, 635, 638, 702
AclWI GGATC 2 cut(s) 902, 962
AcoI YGGCCR 2 cut(s) 244, 975
AcsI RAATTY 2 cut(s) 279, 535
AcvI CACGTG 1 cut(s) 889
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 1 cut(s) 803
AfiI CCNNNNNNNGG 2 cut(s) 207, 821
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 4 cut(s) 294, 364, 599, 658
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 4 cut(s) 114, 266, 504, 672
AluI AGCT 4 cut(s) 114, 266, 504, 672
Alw26I GTCTC 1 cut(s) 698
AlwI GGATC 2 cut(s) 902, 962
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 5 cut(s) 3, 75, 244, 632, 975
ApeKI GCWGC 7 cut(s) 41, 485, 488, 491, 569, 669, 858
ApoI RAATTY 2 cut(s) 279, 535
Asp700I GAANNNNTTC 1 cut(s) 539
AspLEI GCGC 4 cut(s) 442, 464, 897, 899
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 125, 955
AsuII TTCGAA 1 cut(s) 731
BanI GGYRCC 1 cut(s) 517
BbrPI CACGTG 1 cut(s) 889
BbsI GAAGAC 3 cut(s) 615, 636, 740
BbvCI CCTCAGC 1 cut(s) 956
BbvI GCAGC 7 cut(s) 53, 472, 475, 478, 556, 681, 870
BccI CCATC 5 cut(s) 13, 138, 469, 506, 824
BceAI ACGGC 2 cut(s) 47, 990
BcgI CGANNNNNNTGC 2 cut(s) 658, 692
BciT130I CCWGG 1 cut(s) 8
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 698
BfmI CTRYAG 1 cut(s) 900
BlpI GCTNAGC 1 cut(s) 573
Bme1390I CCNGG 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 4 cut(s) 257, 519, 744, 826
BmrFI CCNGG 1 cut(s) 8
BmrI ACTGGG 1 cut(s) 723
BmsI GCATC 1 cut(s) 556
BmuI ACTGGG 1 cut(s) 723
BpiI GAAGAC 3 cut(s) 615, 636, 740
BplI GAGNNNNNCTC 2 cut(s) 62, 94
Bpu10I CCTNAGC 1 cut(s) 956
Bpu1102I GCTNAGC 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 731
Bsa29I ATCGAT 1 cut(s) 581
BsaAI YACGTR 1 cut(s) 889
BsaBI GATNNNNATC 1 cut(s) 945
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 6
BsaWI WCCGGW 5 cut(s) 258, 268, 433, 626, 696
BsaXI ACNNNNNCTCC 2 cut(s) 935, 965
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 821
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 2 cut(s) 136, 718
Bse8I GATNNNNATC 1 cut(s) 945
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 1 cut(s) 8
BseCI ATCGAT 1 cut(s) 581
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 2 cut(s) 571, 984
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 2 cut(s) 207, 821
BseMII CTCAG 3 cut(s) 456, 564, 970
BseNI ACTGG 2 cut(s) 136, 718
BsePI GCGCGC 1 cut(s) 895
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 7 cut(s) 53, 472, 475, 478, 556, 681, 870
BseYI CCCAGC 1 cut(s) 560
BsgI GTGCAG 1 cut(s) 688
Bsh1236I CGCG 2 cut(s) 533, 897
BshFI GGCC 5 cut(s) 5, 77, 246, 634, 977
BshNI GGYRCC 1 cut(s) 517
BshVI ATCGAT 1 cut(s) 581
BsiSI CCGG 7 cut(s) 84, 259, 269, 383, 434, 627, 697
BslI CCNNNNNNNGG 2 cut(s) 207, 821
BsmAI GTCTC 1 cut(s) 698
BsmBI CGTCTC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 5 cut(s) 5, 77, 246, 634, 977
Bsp119I TTCGAA 1 cut(s) 731
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 6 cut(s) 310, 582, 907, 921, 940, 967
Bsp1720I GCTNAGC 1 cut(s) 573
BspACI CCGC 9 cut(s) 74, 81, 185, 398, 407, 531, 635, 638, 702
BspANI GGCC 5 cut(s) 5, 77, 246, 634, 977
BspCNI CTCAG 3 cut(s) 457, 565, 969
BspDI ATCGAT 1 cut(s) 581
BspEI TCCGGA 1 cut(s) 258
BspFNI CGCG 2 cut(s) 533, 897
BspLI GGNNCC 4 cut(s) 257, 519, 744, 826
BspPI GGATC 2 cut(s) 902, 962
BspT104I TTCGAA 1 cut(s) 731
BspT107I GGYRCC 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 2 cut(s) 136, 718
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 1 cut(s) 6
BssHII GCGCGC 1 cut(s) 895
BssMI GATC 6 cut(s) 310, 582, 907, 921, 940, 967
BssNI GRCGYC 1 cut(s) 380
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 4 cut(s) 35, 374, 728, 806
Bst6I CTCTTC 3 cut(s) 41, 592, 653
BstACI GRCGYC 1 cut(s) 380
BstBAI YACGTR 1 cut(s) 889
BstBI TTCGAA 1 cut(s) 731
BstC8I GCNNGC 3 cut(s) 880, 884, 897
BstDEI CTNAG 3 cut(s) 465, 573, 956
BstEII GGTNACC 1 cut(s) 271
BstF5I GGATG 2 cut(s) 571, 984
BstFNI CGCG 2 cut(s) 533, 897
BstHHI GCGC 4 cut(s) 442, 464, 897, 899
BstKTI GATC 6 cut(s) 313, 585, 910, 924, 943, 970
BstMAI GTCTC 1 cut(s) 698
BstMBI GATC 6 cut(s) 310, 582, 907, 921, 940, 967
BstMWI GCNNNNNNNGC 7 cut(s) 80, 191, 406, 491, 566, 879, 892
BstNI CCWGG 1 cut(s) 8
BstNSI RCATGY 1 cut(s) 613
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 6
BstSFI CTRYAG 1 cut(s) 900
BstUI CGCG 2 cut(s) 533, 897
BstV1I GCAGC 7 cut(s) 53, 472, 475, 478, 556, 681, 870
BstV2I GAAGAC 3 cut(s) 615, 636, 740
Bsu15I ATCGAT 1 cut(s) 581
BsuRI GGCC 5 cut(s) 5, 77, 246, 634, 977
BsuTUI ATCGAT 1 cut(s) 581
BtsCI GGATG 2 cut(s) 571, 984
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 201, 724
Cac8I GCNNGC 3 cut(s) 880, 884, 897
CfoI GCGC 4 cut(s) 442, 464, 897, 899
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 581
CseI GACGC 3 cut(s) 85, 388, 949
Csp6I GTAC 1 cut(s) 802
CviAII CATG 4 cut(s) 190, 308, 610, 916
CviQI GTAC 1 cut(s) 802
DdeI CTNAG 3 cut(s) 465, 573, 956
DpnI GATC 6 cut(s) 312, 584, 909, 923, 942, 969
DpnII GATC 6 cut(s) 310, 582, 907, 921, 940, 967
EaeI YGGCCR 2 cut(s) 244, 975
Eam1104I CTCTTC 3 cut(s) 41, 592, 653
EarI CTCTTC 3 cut(s) 41, 592, 653
EciI GGCGGA 3 cut(s) 70, 200, 387
Eco72I CACGTG 1 cut(s) 889
Eco91I GGTNACC 1 cut(s) 271
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 698
FaeI CATG 4 cut(s) 193, 311, 613, 919
FaiI YATR 6 cut(s) 174, 191, 309, 611, 758, 917
FatI CATG 4 cut(s) 189, 307, 609, 915
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 2 cut(s) 678, 903
FokI GGATG 2 cut(s) 578, 971
GlaI GCGC 4 cut(s) 441, 463, 896, 898
GsaI CCCAGC 1 cut(s) 564
HaeIII GGCC 5 cut(s) 5, 77, 246, 634, 977
HapII CCGG 7 cut(s) 84, 259, 269, 383, 434, 627, 697
HgaI GACGC 3 cut(s) 85, 388, 949
HhaI GCGC 4 cut(s) 442, 464, 897, 899
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 4 cut(s) 193, 311, 613, 919
Hin6I GCGC 4 cut(s) 440, 462, 895, 897
HinP1I GCGC 4 cut(s) 440, 462, 895, 897
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HinfI GANTC 3 cut(s) 87, 240, 992
HpaII CCGG 7 cut(s) 84, 259, 269, 383, 434, 627, 697
HphI GGTGA 2 cut(s) 125, 955
Hpy166II GTNNAC 3 cut(s) 370, 679, 904
Hpy188I TCNGA 4 cut(s) 285, 301, 341, 378
Hpy188III TCNNGA 7 cut(s) 91, 100, 208, 259, 314, 925, 999
Hpy8I GTNNAC 3 cut(s) 370, 679, 904
Hpy99I CGWCG 6 cut(s) 101, 151, 382, 652, 655, 680
HpyAV CCTTC 7 cut(s) 61, 205, 300, 339, 404, 522, 929
HpyCH4III ACNGT 4 cut(s) 35, 374, 728, 806
HpyCH4IV ACGT 1 cut(s) 888
HpyCH4V TGCA 4 cut(s) 41, 669, 749, 878
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 191, 406, 491, 566, 879, 892
HpyF3I CTNAG 3 cut(s) 465, 573, 956
HpySE526I ACGT 1 cut(s) 888
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 4 cut(s) 193, 311, 613, 919
HspAI GCGC 4 cut(s) 440, 462, 895, 897
Kpn2I TCCGGA 1 cut(s) 258
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 6 cut(s) 310, 582, 907, 921, 940, 967
LmnI GCTCC 3 cut(s) 271, 677, 892
Lsp1109I GCAGC 7 cut(s) 53, 472, 475, 478, 556, 681, 870
LweI GCATC 1 cut(s) 556
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 4 cut(s) 196, 271, 925, 961
MalI GATC 6 cut(s) 312, 584, 909, 923, 942, 969
MboI GATC 6 cut(s) 310, 582, 907, 921, 940, 967
MluCI AATT 6 cut(s) 279, 294, 535, 599, 750, 761
MmeI TCCRAC 4 cut(s) 207, 308, 401, 995
MroI TCCGGA 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 539
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 2 cut(s) 194, 608
MspA1I CMGCKG 1 cut(s) 640
MspI CCGG 7 cut(s) 84, 259, 269, 383, 434, 627, 697
MspR9I CCNGG 1 cut(s) 8
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 1 cut(s) 8
MvnI CGCG 2 cut(s) 533, 897
MwoI GCNNNNNNNGC 7 cut(s) 80, 191, 406, 491, 566, 879, 892
NdeII GATC 6 cut(s) 310, 582, 907, 921, 940, 967
NlaIII CATG 4 cut(s) 193, 311, 613, 919
NlaIV GGNNCC 4 cut(s) 257, 519, 744, 826
NmuCI GTSAC 3 cut(s) 196, 925, 961
NspI RCATGY 1 cut(s) 613
NspV TTCGAA 1 cut(s) 731
PauI GCGCGC 1 cut(s) 895
PciI ACATGT 1 cut(s) 609
PcsI WCGNNNNNNNCGW 2 cut(s) 146, 930
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 539
PfeI GAWTC 3 cut(s) 87, 240, 992
PmaCI CACGTG 1 cut(s) 889
PmlI CACGTG 1 cut(s) 889
Ppu21I YACGTR 1 cut(s) 889
PscI ACATGT 1 cut(s) 609
Psp6I CCWGG 1 cut(s) 6
PspCI CACGTG 1 cut(s) 889
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 4 cut(s) 257, 519, 744, 826
PspPI GGNCC 1 cut(s) 4
PteI GCGCGC 1 cut(s) 895
RsaI GTAC 1 cut(s) 803
RsaNI GTAC 1 cut(s) 802
RseI CAYNNNNRTG 2 cut(s) 194, 608
SalI GTCGAC 1 cut(s) 677
SaqAI TTAA 1 cut(s) 554
Sau3AI GATC 6 cut(s) 310, 582, 907, 921, 940, 967
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 8
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 1 cut(s) 900
SfuI TTCGAA 1 cut(s) 731
SmiMI CAYNNNNRTG 2 cut(s) 194, 608
Sse9I AATT 6 cut(s) 279, 294, 535, 599, 750, 761
SsiI CCGC 9 cut(s) 74, 81, 185, 398, 407, 531, 635, 638, 702
StyD4I CCNGG 1 cut(s) 6
TaaI ACNGT 4 cut(s) 35, 374, 728, 806
TaiI ACGT 1 cut(s) 891
TaqI TCGA 6 cut(s) 99, 149, 581, 678, 731, 990
TasI AATT 6 cut(s) 279, 294, 535, 599, 750, 761
TauI GCSGC 6 cut(s) 77, 409, 533, 637, 640, 705
TfiI GAWTC 3 cut(s) 87, 240, 992
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 2 cut(s) 201, 731
TseFI GTSAC 3 cut(s) 196, 925, 961
TseI GCWGC 7 cut(s) 41, 485, 488, 491, 569, 669, 858
Tsp45I GTSAC 3 cut(s) 196, 925, 961
TspDTI ATGAA 4 cut(s) 177, 528, 532, 773
TspGWI ACGGA 3 cut(s) 170, 182, 664
TspRI CASTG 2 cut(s) 201, 731
XapI RAATTY 2 cut(s) 279, 535
XceI RCATGY 1 cut(s) 613
XmiI GTMKAC 2 cut(s) 678, 903
XmnI GAANNNNTTC 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.