pycom15g07970

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002837
Physical Location & Seq
Reverse (-)
4941201 .. 4942103
903 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 597 bp
ATGGAGGTAAAAACAAAATATTTACGAACTCGAAAAAAAAAAAAAAAGAATAAATATTTACTGCTGGGTGCAGACCATTTCTCAAGATTCACACTGTTGCCACCATTTCGCTTCATCCTTCCACCCAAAAACCAAAAAAACCAGAGAGAGACAGAGAAAGTCCGAGAAAATTTTCTGTTTGGTTCTCGGAAAATGGTGATGGCAAAGTCTAAATTGTCGTACGAGGAGTCTCGCCAGCAGCGATTGGAGGAAAACAAAAAGAGAATGGAAGCTCTCAATCTGCCTATGCTTGCTCAAGCTCTTAACAAAACCCCAAATTCTCCCAAACCCTCCCCTATGAAGCGCGCTGGGAAGCCTCGCACGTATGAGGTGCAGATGGTGGAGGTGAGGAGGTCCACCCGGGTTACAAATCGACCTGCACCCGATTACAAAGAAGTTGTGGCGGAGCGAGTAATGATTCCGAGAAGGAGCTACGCTACTAATCGGCATAGGGATTTGGCGAACCGGGTGTATGCTTCCGATGAAGCCAGAGCCGCCGCGATGGTGAAAGCCGACGAATTAGAGGCCAGTTTGGGATCGATCACCCGACACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

199

Amino Acids

23.19

Weight (kDa)

10.59

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 424
AccII CGCG 2 cut(s) 345, 539
AciI CCGC 3 cut(s) 443, 534, 537
AclWI GGATC 1 cut(s) 583
AcsI RAATTY 2 cut(s) 169, 316
AfaI GTAC 1 cut(s) 221
AluBI AGCT 3 cut(s) 272, 299, 471
AluI AGCT 3 cut(s) 272, 299, 471
Alw26I GTCTC 2 cut(s) 143, 234
AlwI GGATC 1 cut(s) 583
Ama87I CYCGRG 1 cut(s) 399
AoxI GGCC 1 cut(s) 564
ApeKI GCWGC 1 cut(s) 238
ApoI RAATTY 2 cut(s) 169, 316
Asp700I GAANNNNTTC 1 cut(s) 171
AspLEI GCGC 2 cut(s) 345, 347
AspS9I GGNCC 1 cut(s) 393
AsuC2I CCSGG 3 cut(s) 400, 401, 506
AsuHPI GGTGA 4 cut(s) 208, 397, 556, 574
AvaI CYCGRG 1 cut(s) 399
AvaII GGWCC 1 cut(s) 393
BbvI GCAGC 1 cut(s) 250
BccI CCATC 3 cut(s) 193, 370, 535
BcnI CCSGG 3 cut(s) 400, 401, 506
BcoDI GTCTC 2 cut(s) 143, 234
BfuAI ACCTGC 1 cut(s) 424
BisI GCNGC 3 cut(s) 239, 534, 537
BlsI GCNGC 3 cut(s) 240, 535, 538
Bme1390I CCNGG 3 cut(s) 400, 401, 506
Bme18I GGWCC 1 cut(s) 393
BmeT110I CYCGRG 1 cut(s) 399
BmgT120I GGNCC 1 cut(s) 393
BmrFI CCNGG 3 cut(s) 400, 401, 506
BpuEI CTTGAG 2 cut(s) 67, 279
BpuMI CCSGG 3 cut(s) 400, 401, 506
Bsa29I ATCGAT 1 cut(s) 578
BsaAI YACGTR 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 567
BseCI ATCGAT 1 cut(s) 578
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 567
BsePI GCGCGC 1 cut(s) 343
BseRI GAGGAG 2 cut(s) 239, 403
BseXI GCAGC 1 cut(s) 250
BseYI CCCAGC 2 cut(s) 64, 347
BsgI GTGCAG 3 cut(s) 90, 392, 402
Bsh1236I CGCG 2 cut(s) 345, 539
BshFI GGCC 1 cut(s) 566
BshVI ATCGAT 1 cut(s) 578
BsiHKCI CYCGRG 1 cut(s) 399
BsiSI CCGG 2 cut(s) 400, 505
BsiWI CGTACG 1 cut(s) 219
BsmAI GTCTC 2 cut(s) 143, 234
BsnI GGCC 1 cut(s) 566
BsoBI CYCGRG 1 cut(s) 399
Bsp143I GATC 2 cut(s) 575, 579
BspACI CCGC 3 cut(s) 443, 534, 537
BspANI GGCC 1 cut(s) 566
BspDI ATCGAT 1 cut(s) 578
BspFNI CGCG 2 cut(s) 345, 539
BspMI ACCTGC 1 cut(s) 424
BspPI GGATC 1 cut(s) 583
BsrI ACTGG 1 cut(s) 567
BssECI CCNNGG 1 cut(s) 399
BssHII GCGCGC 1 cut(s) 343
BssMI GATC 2 cut(s) 575, 579
Bst4CI ACNGT 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 3 cut(s) 236, 291, 345
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 2 cut(s) 345, 539
BstHHI GCGC 2 cut(s) 345, 347
BstKTI GATC 2 cut(s) 578, 582
BstMAI GTCTC 2 cut(s) 143, 234
BstMBI GATC 2 cut(s) 575, 579
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstSCI CCNGG 3 cut(s) 398, 399, 504
BstUI CGCG 2 cut(s) 345, 539
BstV1I GCAGC 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 578
BsuRI GGCC 1 cut(s) 566
BsuTUI ATCGAT 1 cut(s) 578
BtgZI GCGATG 1 cut(s) 554
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 92
BveI ACCTGC 1 cut(s) 424
Cac8I GCNNGC 3 cut(s) 236, 291, 345
CfoI GCGC 2 cut(s) 345, 347
Cfr13I GGNCC 1 cut(s) 393
Cfr9I CCCGGG 1 cut(s) 399
ClaI ATCGAT 1 cut(s) 578
Csp6I GTAC 1 cut(s) 220
CviJI RGCY 8 cut(s) 272, 299, 355, 471, 527, 533, 551, 566
CviKI_1 RGCY 8 cut(s) 272, 299, 355, 471, 527, 533, 551, 566
CviQI GTAC 1 cut(s) 220
DpnI GATC 2 cut(s) 577, 581
DpnII GATC 2 cut(s) 575, 579
EciI GGCGGA 1 cut(s) 458
Eco47I GGWCC 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 399
FaiI YATR 5 cut(s) 287, 338, 366, 489, 513
Fnu4HI GCNGC 3 cut(s) 239, 534, 537
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 3 cut(s) 239, 534, 537
GlaI GCGC 2 cut(s) 344, 346
GluI GCNGC 3 cut(s) 239, 534, 537
GsaI CCCAGC 2 cut(s) 68, 351
HaeIII GGCC 1 cut(s) 566
HapII CCGG 2 cut(s) 400, 505
HhaI GCGC 2 cut(s) 345, 347
Hin6I GCGC 2 cut(s) 343, 345
HinP1I GCGC 2 cut(s) 343, 345
HinfI GANTC 3 cut(s) 87, 227, 457
HpaII CCGG 2 cut(s) 400, 505
HphI GGTGA 4 cut(s) 208, 397, 556, 574
Hpy166II GTNNAC 1 cut(s) 396
Hpy188I TCNGA 4 cut(s) 164, 189, 462, 520
Hpy188III TCNNGA 1 cut(s) 84
Hpy8I GTNNAC 1 cut(s) 396
Hpy99I CGWCG 1 cut(s) 557
HpyAV CCTTC 2 cut(s) 128, 459
HpyCH4III ACNGT 1 cut(s) 96
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 3 cut(s) 71, 373, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpySE526I ACGT 1 cut(s) 362
HspAI GCGC 2 cut(s) 343, 345
Kzo9I GATC 2 cut(s) 575, 579
LmnI GCTCC 2 cut(s) 445, 468
LpnPI CCDG 9 cut(s) 50, 155, 248, 333, 413, 429, 518, 541, 580
Lsp1109I GCAGC 1 cut(s) 250
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 1 cut(s) 403
MalI GATC 2 cut(s) 577, 581
MboI GATC 2 cut(s) 575, 579
MluCI AATT 4 cut(s) 169, 212, 316, 557
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 9 cut(s) 217, 241, 340, 361, 366, 376, 381, 384, 556
MroXI GAANNNNTTC 1 cut(s) 171
MseI TTAA 1 cut(s) 303
MspI CCGG 2 cut(s) 400, 505
MspR9I CCNGG 3 cut(s) 400, 401, 506
MvnI CGCG 2 cut(s) 345, 539
MwoI GCNNNNNNNGC 1 cut(s) 533
NciI CCSGG 3 cut(s) 400, 401, 506
NdeII GATC 2 cut(s) 575, 579
PauI GCGCGC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 2 cut(s) 87, 457
Pfl23II CGTACG 1 cut(s) 219
PkrI GCNGC 3 cut(s) 240, 535, 538
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 363
PspFI CCCAGC 2 cut(s) 64, 347
PspLI CGTACG 1 cut(s) 219
PspPI GGNCC 1 cut(s) 393
PteI GCGCGC 1 cut(s) 343
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 3 cut(s) 239, 534, 537
Sau3AI GATC 2 cut(s) 575, 579
Sau96I GGNCC 1 cut(s) 393
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 3 cut(s) 400, 401, 506
SetI ASST 9 cut(s) 9, 274, 301, 365, 372, 387, 395, 418, 473
SinI GGWCC 1 cut(s) 393
SmaI CCCGGG 1 cut(s) 401
SmlI CTYRAG 2 cut(s) 82, 294
SmoI CTYRAG 2 cut(s) 82, 294
Sse9I AATT 4 cut(s) 169, 212, 316, 557
SsiI CCGC 3 cut(s) 443, 534, 537
SspI AATATT 2 cut(s) 20, 56
StyD4I CCNGG 3 cut(s) 398, 399, 504
TaaI ACNGT 1 cut(s) 96
TaiI ACGT 1 cut(s) 365
TaqI TCGA 3 cut(s) 31, 412, 578
TasI AATT 4 cut(s) 169, 212, 316, 557
TauI GCSGC 2 cut(s) 536, 539
TfiI GAWTC 2 cut(s) 87, 457
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 1 cut(s) 99
TseI GCWGC 1 cut(s) 238
TspDTI ATGAA 3 cut(s) 103, 353, 537
TspMI CCCGGG 1 cut(s) 399
TspRI CASTG 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 393
XapI RAATTY 2 cut(s) 169, 316
XmaI CCCGGG 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.