Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0011061

Physical Location & Seq

Genomic Coordinates Reverse (-)

5917294 .. 5918892

Gene Structure 1599 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g09170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1599 bp

ATGTATGGCAAATGCGGGAGAATGGGGATTGCCTTTGAGGTGTTTGATAGAATGCCTGAGAGAAATGTTGTTTCGTGGACGGCTGTAATGTGTGGATACTTACAGAATGGTAATGCCAAAGGCTCACTGTCTCTATTTAGTAGAATGGGGTTTTCGGGAATTAAGCCAAATGAGTTCACCTTTTCGATCAATCTTAAGGCGTCTGGGTTTGTGGGCATTGTTGAGAATGGAATGCAGATTCACAATATGTGCACCAAATCTGGTTTTGAATGGGTTACAGTGGTGAGCAATTCTATCCTCGACATGTATGCGAAATGTGGAAGATTTCACGAAGCAGCACGAATGTTCGATGTTATGCCAGTTAAGAATCTTATCAGTTGGAACGCGATGATAGCTGGATTCACACTTGAAGGAAATGGGGAGAGAGCCTTGCTCTTATTTAGGAAAATGAAAGGGCTAGGAGAAGTCCCCGACGAATATACGATTACAACTACGCTGAAAGCTTGCAGTGGTCTTGGAGCAGTCCGGCAAGGAAGCCAAATACATTCCTCCTTAATCACAAGAGGATTCCTGTGCTCAGTTCGGACTACTATTGCTGGTGCTCTTGTCGATCTGTACGTAAAATGCAGGCACTTAACTGAAGCTCAGAGGGTATTCGATCAAATTGAACAGAAGAATTTGGTATCCTGGAGTTCCCTGATTCTTGGTTATGCCCAAGAATGCCATCTACTACAAGCCATGGACTTGTTTAGGCAGCTCAGGGTTAGTATCGATCATCAAGTAGACGGGTTTGTTCTCTCAAGCCTTATGGGCGTCTTTGCTGATTTTGCCCTGGTTGAACAAGGTAGGCAAATGCATGCTTTCACTCTCAAAATCCCATCTGGTTTAGACATATCAGTAACTAATTCGATTCTGGATATGTATCTCAAGTGTGGATTAACAGACGAGGCAGAAAGACTGTTTAATGAAACTCCGGTGAGGAATGTAATTTCTTGGACGATTATGATCACAGGCTACGGAAAGCATGGTCTTGGAAGGAAATCTGTACGTCTGTTCCAACAAATGCAATCGGATGACATAGAACCCGATGGGGTGACTTACTTGGCCGTGCTCTCAGCCTGCAGCCATTCTGGACTAATAGAAGAATGCAAAGATTACTTCTCAAGATTGTGTCTCGACAAACGGATCAAACCAATTGTAGAGCATTATGCTTGCATGGTCGATCTCCTCGGCAGAGCTGGACGCGTAAAAGAAGCCAAGAATCTCATCGACAACATGCCTTTGAAACCGAATGTTGGAATACAGCAAACTCTACTTGGTGCTTGTAGAGTACATGGGGACTTGGAAATAGGGAGAGAAGTAGGCGAGACGCTTCTGAAACTGGACAGCAATAACCCTGTCAGCTATGTGATGCTGTCAAACATCTACGCTGAGGCCGGTTACTGGAAAGAGTGCGAGAGAATAAGAAAACTGTTTAAGATGAAAGGGTTAAAGAAAGAGGCAGGACGCAGTTGGGTGGATCGATCAACAAGGGGGTCTACTTCTTCTACAATGGGGATGAGACACACCCGCTTACGGAAAAGATTCATCAAGTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF167 DYW_deaminase PPR PPR_2 Pkinase

Protein Analysis

533

Amino Acids

59.61

Weight (kDa)

9.05

Isoelectric Point (pI)

35.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	21 - 66	2.4e-10	PPR repeat family
PPR_2	PF13041	123 - 169	4.4e-09	PPR repeat family
PPR	PF01535	125 - 154	9.7e-06	PPR repeat
PPR_2	PF13041	327 - 375	9.6e-11	PPR repeat family
PPR	PF01535	330 - 359	7.9e-07	PPR repeat
E_motif	PF20431	445 - 507	1.6e-18	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15130
fragaria_vesca	FvH4_2g36310
malus_domestica	MD08G1120300.v1.1 MD08G1120600.v1.1 MD15G1099700.v1.1
prunus_persica	Prupe.1G454500_v2.0.a1
pyrus_communis	pycom08g10070 pycom08g10090 pycom15g09170
rosa_chinensis	RchiOBHm_Chr6g0305571
rosa_laevigata	RLG00000010876
rosa_multiflora	Rmu_co8434531.1_g000001 Rmu_sc0000841.1_g000001 Rmu_sc0001153.1_g000004 Rmu_sc0033568.1_g000001
rosa_rugosa	Rorug06G0341800
rosa_samantha	Rh6AG453800 Rh6BG447700 Rh6DG454100
rosa_wichuraiana	Rw6G039550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	783, 1538
AccII	CGCG	2 cut(s)	386, 1245
AciI	CCGC	2 cut(s)	15, 1570
AclWI	GGATC	2 cut(s)	1193, 1527
AcoI	YGGCCR	1 cut(s)	1104
AcsI	RAATTY	1 cut(s)	676
AcuI	CTGAAG	1 cut(s)	660
AcyI	GRCGYC	2 cut(s)	200, 813
AfaI	GTAC	3 cut(s)	617, 1047, 1332
AfiI	CCNNNNNNNGG	3 cut(s)	1295, 1443, 1575
AflII	CTTAAG	1 cut(s)	194
AflIII	ACRYGT	2 cut(s)	303, 1243
AgsI	TTSAA	5 cut(s)	269, 410, 668, 839, 1285
AjnI	CCWGG	2 cut(s)	686, 831
AluBI	AGCT	6 cut(s)	395, 503, 644, 757, 1238, 1404
AluI	AGCT	6 cut(s)	395, 503, 644, 757, 1238, 1404
Alw21I	GWGCWC	4 cut(s)	254, 578, 604, 1113
Alw26I	GTCTC	4 cut(s)	135, 1178, 1361, 1555
Alw44I	GTGCAC	1 cut(s)	250
AlwI	GGATC	2 cut(s)	1193, 1527
AoxI	GGCC	2 cut(s)	1104, 1434
ApaLI	GTGCAC	1 cut(s)	250
ApeKI	GCWGC	3 cut(s)	335, 754, 1122
ApoI	RAATTY	1 cut(s)	676
Asp700I	GAANNNNTTC	1 cut(s)	1583
AsuHPI	GGTGA	4 cut(s)	169, 295, 988, 1105
BaeGI	GKGCMC	1 cut(s)	254
Bbv12I	GWGCWC	4 cut(s)	254, 578, 604, 1113
BbvCI	CCTCAGC	1 cut(s)	1431
BbvI	GCAGC	3 cut(s)	347, 766, 1134
BccI	CCATC	3 cut(s)	732, 886, 1082
BceAI	ACGGC	2 cut(s)	96, 1091
BcgI	CGANNNNNNTGC	2 cut(s)	290, 324
BciT130I	CCWGG	2 cut(s)	688, 833
BciVI	GTATCC	2 cut(s)	89, 694
BclI	TGATCA	1 cut(s)	1005
BcoDI	GTCTC	4 cut(s)	135, 1178, 1361, 1555
BfaI	CTAG	1 cut(s)	458
BfmI	CTRYAG	1 cut(s)	1120
BfrI	CTTAAG	1 cut(s)	194
BfuI	GTATCC	2 cut(s)	89, 694
BglI	GCCNNNNNGGC	1 cut(s)	810
BisI	GCNGC	3 cut(s)	336, 755, 1123
BlsI	GCNGC	3 cut(s)	337, 756, 1124
Bme1390I	CCNGG	2 cut(s)	688, 833
BmrFI	CCNGG	2 cut(s)	688, 833
BmsI	GCATC	1 cut(s)	1401
BplI	GAGNNNNNCTC	2 cut(s)	417, 449
BpmI	CTGGAG	1 cut(s)	709
Bpu10I	CCTNAGC	2 cut(s)	758, 1431
BpuEI	CTTGAG	3 cut(s)	784, 911, 1147
Bsa29I	ATCGAT	2 cut(s)	771, 1522
BsaAI	YACGTR	1 cut(s)	619
BsaBI	GATNNNNATC	2 cut(s)	921, 1004
BsaHI	GRCGYC	2 cut(s)	200, 813
BsaJI	CCNNGG	3 cut(s)	738, 831, 1228
BsaWI	WCCGGW	1 cut(s)	973
Bsc4I	CCNNNNNNNGG	3 cut(s)	1295, 1443, 1575
Bse118I	RCCGGY	1 cut(s)	1436
Bse1I	ACTGG	3 cut(s)	359, 1386, 1448
Bse8I	GATNNNNATC	2 cut(s)	921, 1004
BseBI	CCWGG	2 cut(s)	688, 833
BseCI	ATCGAT	2 cut(s)	771, 1522
BseDI	CCNNGG	3 cut(s)	738, 831, 1228
BseGI	GGATG	2 cut(s)	1078, 1563
BseJI	GATNNNNATC	2 cut(s)	921, 1004
BseLI	CCNNNNNNNGG	3 cut(s)	1295, 1443, 1575
BseMII	CTCAG	6 cut(s)	48, 591, 659, 772, 1128, 1422
BseNI	ACTGG	3 cut(s)	359, 1386, 1448
BseRI	GAGGAG	1 cut(s)	1217
BseSI	GKGCMC	1 cut(s)	254
BseXI	GCAGC	3 cut(s)	347, 766, 1134
Bsh1236I	CGCG	2 cut(s)	386, 1245
BshFI	GGCC	2 cut(s)	1106, 1436
BshVI	ATCGAT	2 cut(s)	771, 1522
BsiHKAI	GWGCWC	4 cut(s)	254, 578, 604, 1113
BsiSI	CCGG	3 cut(s)	526, 974, 1437
BslFI	GGGAC	2 cut(s)	452, 1352
BslI	CCNNNNNNNGG	3 cut(s)	1295, 1443, 1575
BsmAI	GTCTC	4 cut(s)	135, 1178, 1361, 1555
BsmBI	CGTCTC	1 cut(s)	1361
BsmFI	GGGAC	2 cut(s)	452, 1352
BsmI	GAATGC	4 cut(s)	57, 237, 725, 1151
BsnI	GGCC	2 cut(s)	1106, 1436
Bsp1286I	GDGCHC	4 cut(s)	254, 578, 604, 1113
Bsp143I	GATC	9 cut(s)	186, 610, 658, 772, 1005, 1185, 1222, 1519, 1523
Bsp19I	CCATGG	1 cut(s)	738
BspACI	CCGC	2 cut(s)	15, 1570
BspANI	GGCC	2 cut(s)	1106, 1436
BspCNI	CTCAG	6 cut(s)	49, 590, 658, 771, 1127, 1423
BspDI	ATCGAT	2 cut(s)	771, 1522
BspFNI	CGCG	2 cut(s)	386, 1245
BspMAI	CTGCAG	1 cut(s)	1124
BspPI	GGATC	2 cut(s)	1193, 1527
BspTI	CTTAAG	1 cut(s)	194
BsrFI	RCCGGY	1 cut(s)	1436
BsrI	ACTGG	3 cut(s)	359, 1386, 1448
BssAI	RCCGGY	1 cut(s)	1436
BssECI	CCNNGG	3 cut(s)	738, 831, 1228
BssMI	GATC	9 cut(s)	186, 610, 658, 772, 1005, 1185, 1222, 1519, 1523
BssNI	GRCGYC	2 cut(s)	200, 813
BssT1I	CCWWGG	1 cut(s)	738
Bst2UI	CCWGG	2 cut(s)	688, 833
Bst4CI	ACNGT	4 cut(s)	129, 280, 960, 1473
BstACI	GRCGYC	2 cut(s)	200, 813
BstAFI	CTTAAG	1 cut(s)	194
BstBAI	YACGTR	1 cut(s)	619
BstC8I	GCNNGC	5 cut(s)	505, 629, 858, 1120, 1213
BstDEI	CTNAG	6 cut(s)	57, 577, 645, 758, 1114, 1431
BstDSI	CCRYGG	1 cut(s)	738
BstF5I	GGATG	2 cut(s)	1078, 1563
BstFNI	CGCG	2 cut(s)	386, 1245
BstKTI	GATC	9 cut(s)	189, 613, 661, 775, 1008, 1188, 1225, 1522, 1526
BstMAI	GTCTC	4 cut(s)	135, 1178, 1361, 1555
BstMBI	GATC	9 cut(s)	186, 610, 658, 772, 1005, 1185, 1222, 1519, 1523
BstMWI	GCNNNNNNNGC	3 cut(s)	392, 810, 827
BstNI	CCWGG	2 cut(s)	688, 833
BstNSI	RCATGY	3 cut(s)	307, 860, 1279
BstSCI	CCNGG	2 cut(s)	686, 831
BstSFI	CTRYAG	1 cut(s)	1120
BstSLI	GKGCMC	1 cut(s)	254
BstSNI	TACGTA	1 cut(s)	619
BstUI	CGCG	2 cut(s)	386, 1245
BstV1I	GCAGC	3 cut(s)	347, 766, 1134
Bsu15I	ATCGAT	2 cut(s)	771, 1522
BsuI	GTATCC	2 cut(s)	89, 694
BsuRI	GGCC	2 cut(s)	1106, 1436
BsuTUI	ATCGAT	2 cut(s)	771, 1522
BtgI	CCRYGG	1 cut(s)	738
BtgZI	GCGATG	1 cut(s)	401
BtsCI	GGATG	2 cut(s)	1078, 1563
BtsI	GCAGTG	1 cut(s)	514
BtsIMutI	CAGTG	3 cut(s)	125, 285, 514
Cac8I	GCNNGC	5 cut(s)	505, 629, 858, 1120, 1213
Cfr10I	RCCGGY	1 cut(s)	1436
ClaI	ATCGAT	2 cut(s)	771, 1522
CseI	GACGC	5 cut(s)	189, 802, 1251, 1378, 1515
Csp6I	GTAC	3 cut(s)	616, 1046, 1331
CviAII	CATG	7 cut(s)	304, 739, 857, 1025, 1216, 1276, 1334
CviQI	GTAC	3 cut(s)	616, 1046, 1331
DdeI	CTNAG	6 cut(s)	57, 577, 645, 758, 1114, 1431
DpnI	GATC	9 cut(s)	188, 612, 660, 774, 1007, 1187, 1224, 1521, 1525
DpnII	GATC	9 cut(s)	186, 610, 658, 772, 1005, 1185, 1222, 1519, 1523
EaeI	YGGCCR	1 cut(s)	1104
Eco105I	TACGTA	1 cut(s)	619
Eco130I	CCWWGG	1 cut(s)	738
Eco57I	CTGAAG	1 cut(s)	660
EcoRII	CCWGG	2 cut(s)	686, 831
EcoT14I	CCWWGG	1 cut(s)	738
EcoT22I	ATGCAT	1 cut(s)	858
ErhI	CCWWGG	1 cut(s)	738
Esp3I	CGTCTC	1 cut(s)	1361
FaeI	CATG	7 cut(s)	307, 742, 860, 1028, 1219, 1279, 1337
FaqI	GGGAC	2 cut(s)	452, 1352
FatI	CATG	7 cut(s)	303, 738, 856, 1024, 1215, 1275, 1333
FauI	CCCGC	2 cut(s)	8, 1577
FbaI	TGATCA	1 cut(s)	1005
FblI	GTMKAC	2 cut(s)	783, 1538
Fnu4HI	GCNGC	3 cut(s)	336, 755, 1123
FokI	GGATG	2 cut(s)	1085, 1570
Fsp4HI	GCNGC	3 cut(s)	336, 755, 1123
FspBI	CTAG	1 cut(s)	458
GluI	GCNGC	3 cut(s)	336, 755, 1123
GsuI	CTGGAG	1 cut(s)	709
HaeIII	GGCC	2 cut(s)	1106, 1436
HapII	CCGG	3 cut(s)	526, 974, 1437
HgaI	GACGC	5 cut(s)	189, 802, 1251, 1378, 1515
Hin1I	GRCGYC	2 cut(s)	200, 813
Hin1II	CATG	7 cut(s)	307, 742, 860, 1028, 1219, 1279, 1337
HindIII	AAGCTT	1 cut(s)	501
HinfI	GANTC	8 cut(s)	238, 367, 399, 567, 700, 910, 1261, 1584
HpaII	CCGG	3 cut(s)	526, 974, 1437
HphI	GGTGA	4 cut(s)	169, 295, 988, 1105
Hpy166II	GTNNAC	5 cut(s)	78, 177, 252, 784, 1539
Hpy188I	TCNGA	4 cut(s)	585, 648, 1072, 1377
Hpy188III	TCNNGA	6 cut(s)	156, 329, 914, 1131, 1164, 1175
Hpy8I	GTNNAC	5 cut(s)	78, 177, 252, 784, 1539
Hpy99I	CGWCG	1 cut(s)	476
HpyAV	CCTTC	2 cut(s)	404, 1029
HpyCH4III	ACNGT	4 cut(s)	129, 280, 960, 1473
HpyCH4IV	ACGT	2 cut(s)	618, 1048
HpyCH4V	TGCA	9 cut(s)	235, 252, 507, 627, 856, 1066, 1122, 1149, 1215
HpyF10VI	GCNNNNNNNGC	3 cut(s)	392, 810, 827
HpyF3I	CTNAG	6 cut(s)	57, 577, 645, 758, 1114, 1431
HpySE526I	ACGT	2 cut(s)	618, 1048
Hsp92I	GRCGYC	2 cut(s)	200, 813
Hsp92II	CATG	7 cut(s)	307, 742, 860, 1028, 1219, 1279, 1337
Ksp22I	TGATCA	1 cut(s)	1005
Kzo9I	GATC	9 cut(s)	186, 610, 658, 772, 1005, 1185, 1222, 1519, 1523
LmnI	GCTCC	1 cut(s)	518
Lsp1109I	GCAGC	3 cut(s)	347, 766, 1134
LweI	GCATC	1 cut(s)	1401
MaeI	CTAG	1 cut(s)	458
MaeII	ACGT	2 cut(s)	618, 1048
MaeIII	GTNAC	4 cut(s)	274, 898, 1093, 1439
MalI	GATC	9 cut(s)	188, 612, 660, 774, 1007, 1187, 1224, 1521, 1525
MboI	GATC	9 cut(s)	186, 610, 658, 772, 1005, 1185, 1222, 1519, 1523
MboII	GAAGA	4 cut(s)	333, 685, 1154, 1536
MfeI	CAATTG	1 cut(s)	1194
MhlI	GDGCHC	4 cut(s)	254, 578, 604, 1113
MluCI	AATT	7 cut(s)	159, 289, 663, 676, 904, 987, 1194
MluI	ACGCGT	1 cut(s)	1243
MmeI	TCCRAC	3 cut(s)	359, 1081, 1276
Mph1103I	ATGCAT	1 cut(s)	858
MroXI	GAANNNNTTC	1 cut(s)	1583
MseI	TTAA	9 cut(s)	162, 195, 363, 554, 635, 938, 963, 1476, 1490
MspCI	CTTAAG	1 cut(s)	194
MspI	CCGG	3 cut(s)	526, 974, 1437
MspR9I	CCNGG	2 cut(s)	688, 833
MunI	CAATTG	1 cut(s)	1194
Mva1269I	GAATGC	4 cut(s)	57, 237, 725, 1151
MvaI	CCWGG	2 cut(s)	688, 833
MvnI	CGCG	2 cut(s)	386, 1245
MwoI	GCNNNNNNNGC	3 cut(s)	392, 810, 827
NcoI	CCATGG	1 cut(s)	738
NdeII	GATC	9 cut(s)	186, 610, 658, 772, 1005, 1185, 1222, 1519, 1523
NlaIII	CATG	7 cut(s)	307, 742, 860, 1028, 1219, 1279, 1337
NmeAIII	GCCGAG	1 cut(s)	1209
NmuCI	GTSAC	1 cut(s)	1093
NsiI	ATGCAT	1 cut(s)	858
NspI	RCATGY	3 cut(s)	307, 860, 1279
PaeI	GCATGC	1 cut(s)	860
PciI	ACATGT	1 cut(s)	303
PcsI	WCGNNNNNNNCGW	1 cut(s)	615
PctI	GAATGC	4 cut(s)	57, 237, 725, 1151
PdmI	GAANNNNTTC	1 cut(s)	1583
PfeI	GAWTC	8 cut(s)	238, 367, 399, 567, 700, 910, 1261, 1584
PfoI	TCCNGGA	1 cut(s)	686
PkrI	GCNGC	3 cut(s)	337, 756, 1124
Ppu21I	YACGTR	1 cut(s)	619
PscI	ACATGT	1 cut(s)	303
Psp6I	CCWGG	2 cut(s)	686, 831
PspGI	CCWGG	2 cut(s)	686, 831
PstI	CTGCAG	1 cut(s)	1124
RsaI	GTAC	3 cut(s)	617, 1047, 1332
RsaNI	GTAC	3 cut(s)	616, 1046, 1331
SaqAI	TTAA	9 cut(s)	162, 195, 363, 554, 635, 938, 963, 1476, 1490
SatI	GCNGC	3 cut(s)	336, 755, 1123
Sau3AI	GATC	9 cut(s)	186, 610, 658, 772, 1005, 1185, 1222, 1519, 1523
ScrFI	CCNGG	2 cut(s)	688, 833
SduI	GDGCHC	4 cut(s)	254, 578, 604, 1113
SfaNI	GCATC	1 cut(s)	1401
SfcI	CTRYAG	1 cut(s)	1120
SmlI	CTYRAG	4 cut(s)	194, 799, 926, 1162
SmoI	CTYRAG	4 cut(s)	194, 799, 926, 1162
SnaBI	TACGTA	1 cut(s)	619
SphI	GCATGC	1 cut(s)	860
Sse9I	AATT	7 cut(s)	159, 289, 663, 676, 904, 987, 1194
SsiI	CCGC	2 cut(s)	15, 1570
SspMI	CTAG	1 cut(s)	458
StyD4I	CCNGG	2 cut(s)	686, 831
StyI	CCWWGG	1 cut(s)	738
TaaI	ACNGT	4 cut(s)	129, 280, 960, 1473
TaiI	ACGT	2 cut(s)	621, 1051
TasI	AATT	7 cut(s)	159, 289, 663, 676, 904, 987, 1194
TatI	WGTACW	1 cut(s)	1330
TfiI	GAWTC	8 cut(s)	238, 367, 399, 567, 700, 910, 1261, 1584
Tru1I	TTAA	9 cut(s)	162, 195, 363, 554, 635, 938, 963, 1476, 1490
Tru9I	TTAA	9 cut(s)	162, 195, 363, 554, 635, 938, 963, 1476, 1490
TscAI	CASTG	3 cut(s)	132, 285, 514
TseFI	GTSAC	1 cut(s)	1093
TseI	GCWGC	3 cut(s)	335, 754, 1122
Tsp45I	GTSAC	1 cut(s)	1093
TspDTI	ATGAA	4 cut(s)	464, 981, 1496, 1576
TspGWI	ACGGA	3 cut(s)	1032, 1198, 1591
TspRI	CASTG	3 cut(s)	132, 285, 514
Vha464I	CTTAAG	1 cut(s)	194
VneI	GTGCAC	1 cut(s)	250
XapI	RAATTY	1 cut(s)	676
XceI	RCATGY	3 cut(s)	307, 860, 1279
XmiI	GTMKAC	2 cut(s)	783, 1538
XmnI	GAANNNNTTC	1 cut(s)	1583
XspI	CTAG	1 cut(s)	458
Zsp2I	ATGCAT	1 cut(s)	858

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.