Rorug06G0341800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011061
Physical Location & Seq
Forward (+)
49244076 .. 49245580
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0341800.1

Sequence Viewer

Length: 744 bp
ATGAAGACACGGTTGAGCCGGAATAATGACTTGGAAGCCGGAGAGATCCCTTTACTCTACCCTTCGATGACGGAGAGGCCGGAGCTCCGATGGGCCTTCATCCGCAAAGTCTATGCCATCCTCACCTTTCAGATACCCCTCACCATCCCCGTCGCCGCCACCGTCTACTTTAATCCTGCAATCGCCGTATTCCTTATCGAAACTTGGCTCGGATGGTTTCTGAAACAAACGATCATCATCACCTCCCTTGCTGTGCTCGTCGCCATGGTGGTTTGTAGCAAACAGCACCCGCTAAATTACGTGCTCCTTGGGGCTTTCACTATATGTGAGGCCCTCATGGTCGGAGTAAGTTGTGCATTTACCAGCGGGAAGGTGGTTTTGGAGGCTGCGATTCTAACTGCTGTGATTTTTCTAAGTCTGACCTTGTACACCTTTTGCGCCGCAAGGAGAGGCCATGACTTCAACTTCTTGGGTCCTTTCTTGTTTGCATCCCTGCTGGCTCTGCTTGTCTTTGGAGTGATATATATTTTCCTCCCTCTTGGTAAGACTAGTCATATGATATACGGGATTGTGGGAATACTCATATTCTGTGGCTACATTGTCCATGACACAGATGAATTGATCAAGCGCTACGAGTACGATGAGTACATTTTGGCGGCCATCGACTTGTATTTGGACCTCCTCAACTTGTTTCTCAGGTTTCTCGAGTTGATGAAAGATGCTGAGAGCTCAGACGTTGCTTAA

Protein Analysis

247

Amino Acids

27.8

Weight (kDa)

5.56

Isoelectric Point (pI)

29.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bax1-I PF01027 32 - 238 1.6e-49 Inhibitor of apoptosis-promoting Bax1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 165
AciI CCGC 6 cut(s) 103, 156, 290, 366, 441, 656
AclWI GGATC 1 cut(s) 40
AcoI YGGCCR 1 cut(s) 657
AfaI GTAC 3 cut(s) 428, 638, 647
AfeI AGCGCT 1 cut(s) 629
AgsI TTSAA 1 cut(s) 463
AhlI ACTAGT 1 cut(s) 548
AjuI GAANNNNNNNTTGG 4 cut(s) 14, 46, 362, 394
AluBI AGCT 2 cut(s) 85, 729
AluI AGCT 2 cut(s) 85, 729
Alw21I GWGCWC 4 cut(s) 87, 258, 306, 731
AlwI GGATC 1 cut(s) 40
Ama87I CYCGRG 1 cut(s) 704
Aor51HI AGCGCT 1 cut(s) 629
AoxI GGCC 5 cut(s) 77, 93, 330, 451, 657
ApeKI GCWGC 1 cut(s) 386
AspLEI GCGC 2 cut(s) 440, 630
AspS9I GGNCC 4 cut(s) 93, 331, 473, 676
AsuHPI GGTGA 3 cut(s) 115, 133, 232
AvaI CYCGRG 1 cut(s) 704
AvaII GGWCC 2 cut(s) 473, 676
BanII GRGCYC 2 cut(s) 87, 731
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 4 cut(s) 87, 258, 306, 731
BbvI GCAGC 1 cut(s) 373
BccI CCATC 5 cut(s) 84, 125, 152, 207, 668
BceAI ACGGC 1 cut(s) 170
BclI TGATCA 1 cut(s) 621
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 1 cut(s) 549
BfoI RGCGCY 1 cut(s) 631
BisI GCNGC 4 cut(s) 156, 387, 441, 657
BlsI GCNGC 4 cut(s) 157, 388, 442, 658
Bme18I GGWCC 2 cut(s) 473, 676
BmeT110I CYCGRG 1 cut(s) 704
BmgT120I GGNCC 4 cut(s) 93, 331, 473, 676
BmiI GGNNCC 1 cut(s) 474
BmsI GCATC 2 cut(s) 497, 709
BpiI GAAGAC 1 cut(s) 11
BsaAI YACGTR 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 264, 307
Bse8I GATNNNNATC 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 264, 307
BseGI GGATG 5 cut(s) 99, 117, 144, 218, 488
BseJI GATNNNNATC 1 cut(s) 236
BseMII CTCAG 3 cut(s) 709, 714, 744
BseRI GAGGAG 1 cut(s) 671
BseXI GCAGC 1 cut(s) 373
BshFI GGCC 5 cut(s) 79, 95, 332, 453, 659
BsiHKAI GWGCWC 4 cut(s) 87, 258, 306, 731
BsiHKCI CYCGRG 1 cut(s) 704
BsiSI CCGG 3 cut(s) 19, 39, 80
BsnI GGCC 5 cut(s) 79, 95, 332, 453, 659
BsoBI CYCGRG 1 cut(s) 704
Bsp1286I GDGCHC 4 cut(s) 87, 258, 306, 731
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 3 cut(s) 45, 231, 621
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 6 cut(s) 103, 156, 290, 366, 441, 656
BspANI GGCC 5 cut(s) 79, 95, 332, 453, 659
BspCNI CTCAG 3 cut(s) 708, 715, 743
BspLI GGNNCC 1 cut(s) 474
BspPI GGATC 1 cut(s) 40
BsrGI TGTACA 1 cut(s) 426
BssECI CCNNGG 2 cut(s) 264, 307
BssMI GATC 3 cut(s) 45, 231, 621
BssT1I CCWWGG 2 cut(s) 264, 307
Bst4CI ACNGT 2 cut(s) 12, 163
BstAUI TGTACA 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 4 cut(s) 413, 695, 723, 730
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 5 cut(s) 99, 117, 144, 218, 488
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 2 cut(s) 440, 630
BstKTI GATC 3 cut(s) 48, 234, 624
BstMBI GATC 3 cut(s) 45, 231, 621
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BsuRI GGCC 5 cut(s) 79, 95, 332, 453, 659
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 5 cut(s) 99, 117, 144, 218, 488
Cac8I GCNNGC 1 cut(s) 498
CfoI GCGC 2 cut(s) 440, 630
Cfr13I GGNCC 4 cut(s) 93, 331, 473, 676
Csp6I GTAC 3 cut(s) 427, 637, 646
CviAII CATG 4 cut(s) 265, 337, 455, 605
CviQI GTAC 3 cut(s) 427, 637, 646
DdeI CTNAG 4 cut(s) 413, 695, 723, 730
DpnI GATC 3 cut(s) 47, 233, 623
DpnII GATC 3 cut(s) 45, 231, 621
EaeI YGGCCR 1 cut(s) 657
Ecl136II GAGCTC 2 cut(s) 85, 729
Eco130I CCWWGG 2 cut(s) 264, 307
Eco24I GRGCYC 2 cut(s) 87, 731
Eco47I GGWCC 2 cut(s) 473, 676
Eco47III AGCGCT 1 cut(s) 629
Eco53kI GAGCTC 2 cut(s) 85, 729
Eco88I CYCGRG 1 cut(s) 704
EcoICRI GAGCTC 2 cut(s) 85, 729
EcoO109I RGGNCCY 2 cut(s) 331, 473
EcoT14I CCWWGG 2 cut(s) 264, 307
EcoT38I GRGCYC 2 cut(s) 87, 731
ErhI CCWWGG 2 cut(s) 264, 307
FaeI CATG 4 cut(s) 268, 340, 458, 608
FatI CATG 4 cut(s) 264, 336, 454, 604
FauI CCCGC 2 cut(s) 297, 359
FauNDI CATATG 1 cut(s) 555
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 4 cut(s) 156, 387, 441, 657
FokI GGATG 5 cut(s) 86, 104, 131, 225, 475
FriOI GRGCYC 2 cut(s) 87, 731
Fsp4HI GCNGC 4 cut(s) 156, 387, 441, 657
FspBI CTAG 1 cut(s) 549
GlaI GCGC 2 cut(s) 439, 629
GluI GCNGC 4 cut(s) 156, 387, 441, 657
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 5 cut(s) 79, 95, 332, 453, 659
HapII CCGG 3 cut(s) 19, 39, 80
HhaI GCGC 2 cut(s) 440, 630
Hin1II CATG 4 cut(s) 268, 340, 458, 608
Hin6I GCGC 2 cut(s) 438, 628
HinP1I GCGC 2 cut(s) 438, 628
HinfI GANTC 1 cut(s) 391
HpaII CCGG 3 cut(s) 19, 39, 80
HphI GGTGA 3 cut(s) 115, 133, 232
Hpy166II GTNNAC 2 cut(s) 166, 429
Hpy188I TCNGA 7 cut(s) 89, 132, 212, 222, 344, 420, 733
Hpy188III TCNNGA 1 cut(s) 704
Hpy8I GTNNAC 2 cut(s) 166, 429
Hpy99I CGWCG 2 cut(s) 155, 263
HpyAV CCTTC 3 cut(s) 72, 106, 364
HpyCH4III ACNGT 2 cut(s) 12, 163
HpyCH4IV ACGT 2 cut(s) 300, 735
HpyCH4V TGCA 3 cut(s) 179, 356, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpyF3I CTNAG 4 cut(s) 413, 695, 723, 730
HpySE526I ACGT 2 cut(s) 300, 735
Hsp92II CATG 4 cut(s) 268, 340, 458, 608
HspAI GCGC 2 cut(s) 438, 628
Ksp22I TGATCA 1 cut(s) 621
Kzo9I GATC 3 cut(s) 45, 231, 621
LmnI GCTCC 3 cut(s) 82, 90, 309
LpnPI CCDG 8 cut(s) 32, 52, 93, 189, 376, 482, 506, 682
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 2 cut(s) 497, 709
MaeI CTAG 1 cut(s) 549
MaeII ACGT 2 cut(s) 300, 735
MalI GATC 3 cut(s) 47, 233, 623
MboI GATC 3 cut(s) 45, 231, 621
MboII GAAGA 1 cut(s) 16
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 4 cut(s) 87, 258, 306, 731
MluCI AATT 2 cut(s) 295, 617
MmeI TCCRAC 1 cut(s) 322
MseI TTAA 2 cut(s) 171, 742
MspA1I CMGCKG 1 cut(s) 366
MspI CCGG 3 cut(s) 19, 39, 80
MwoI GCNNNNNNNGC 1 cut(s) 502
NcoI CCATGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 555
NdeII GATC 3 cut(s) 45, 231, 621
NlaIII CATG 4 cut(s) 268, 340, 458, 608
NlaIV GGNNCC 1 cut(s) 474
PaeR7I CTCGAG 1 cut(s) 704
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 4 cut(s) 157, 388, 442, 658
Ppu21I YACGTR 1 cut(s) 301
PpuMI RGGWCCY 1 cut(s) 473
Psp124BI GAGCTC 2 cut(s) 87, 731
Psp5II RGGWCCY 1 cut(s) 473
PspN4I GGNNCC 1 cut(s) 474
PspPI GGNCC 4 cut(s) 93, 331, 473, 676
PspPPI RGGWCCY 1 cut(s) 473
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 3 cut(s) 428, 638, 647
RsaNI GTAC 3 cut(s) 427, 637, 646
SacI GAGCTC 2 cut(s) 87, 731
SaqAI TTAA 2 cut(s) 171, 742
SatI GCNGC 4 cut(s) 156, 387, 441, 657
Sau3AI GATC 3 cut(s) 45, 231, 621
Sau96I GGNCC 4 cut(s) 93, 331, 473, 676
SduI GDGCHC 4 cut(s) 87, 258, 306, 731
SfaNI GCATC 2 cut(s) 497, 709
Sfr274I CTCGAG 1 cut(s) 704
SinI GGWCC 2 cut(s) 473, 676
SlaI CTCGAG 1 cut(s) 704
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 2 cut(s) 295, 617
SsiI CCGC 6 cut(s) 103, 156, 290, 366, 441, 656
SspMI CTAG 1 cut(s) 549
SstI GAGCTC 2 cut(s) 87, 731
StyI CCWWGG 2 cut(s) 264, 307
TaaI ACNGT 2 cut(s) 12, 163
TaiI ACGT 2 cut(s) 303, 738
TaqI TCGA 4 cut(s) 65, 198, 663, 705
TasI AATT 2 cut(s) 295, 617
TatI WGTACW 2 cut(s) 426, 645
TauI GCSGC 3 cut(s) 158, 443, 659
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 2 cut(s) 171, 742
Tru9I TTAA 2 cut(s) 171, 742
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 4 cut(s) 17, 88, 630, 728
TspGWI ACGGA 1 cut(s) 86
VpaK11BI GGWCC 2 cut(s) 473, 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 704
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.