RchiOBHm_Chr6g0305571

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011061
Physical Location & Seq
Forward (+)
64522946 .. 64525652
2707 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27462

Sequence Viewer

Length: 2061 bp
ATGAATGAGAGGCAGAGATTGGCGAATCTCCTGCGGAACTGTTCTAAGAATTTGCTACTTCGTGAGGGATTGCAAGCTCATGGGTCAGTGATGAGAATGGGACTTGGGTTTGATTTGATGCTGAACAATGATCTTATAGATATGTATGGTAAGTGTGGTAGAATGGGGAGTGCACGTGAGGTGTTTGATAGAATGCCTGAGAGAAATGTGGTTTCCTGGACTGCTTTAATGTGTGGCTACTTAAACAGTGGCAATGCCAAAGGGTCATTATCTCTGTTCAGTGAAATGGGATTTTCGGGAGTTAAGCCGAATGAGTACACTTTCTCGACTAATCTCAAGGCGTCCGGGATCTTGGGGATTGCTGATAATGGAATGCAGATACACAGTATGTGCGTTAAGTCTGGTTATGAATGGGCTGCAGTGGTGAGCAATGCTATCCTTGACATGTATGTGAAATGTGGAAGAGTTAGTGAAGCGTCCAGGATGTTCAGTGTTATGCCGGCTAAGAATGTTATTACTTGGAACGCAATGATAGCTGGATGCACGGTTGAAGGAAATGGGGAGAAAGCGTTGGTATTGTTTAGGAAAATGCAAGAGCTGGGAGAAGTTCCGGATGAGTATACGATTACAAGCACACTCAAGGCTTGTAGTGGCCTTGGAGCAATTCGGCAAGGAAGCCAAATACATTCCTCCTTGATCACAAGGGGGTTTCTGTGTTCAGTTCGGACGATCATTGCAGGTGCTCTCGTTGATATGTATGTGAAGTGCAGGCACTTAACTGAAGCTCAGAGAGTGTTTGATCAAGTTGAACAGAAGAATCTAATATCTTGGAGTGCCCTGATTCTTGGTTATGCCCAAGAATGCAATTTAACACAGGCCCTGGAGTTATTTAGGCAGCTCAGAGCTAGTGTATGTCATCAAGTAGATGGGTTTGTTCTGTCGAGCCTTATGGGTGTGTTTGCTGATTTTGCTCTAGTAGAACAAGGTAGGCAAATGCATGCTTTCACCACTAAAATCCCATCTGGTTTAGACATATCGGTGTTTAACTCGATTCTGGATATGTATCTCAAGTGTGGTTTGACAGATGAAGCAGAAAGACTTTTTGATGAAACTCAGTTGAGAAATGTAATTTCTTGGACAATTATGATCACCGGGTTTGGAAAGCATGGTCTAGGGAAGAAAGCTGTGCATCTTTTCAACCAAATGCAATCGGCGGAAATAGAACCTGATGGAGTGACTTACTTGGCAGTGCTCTCTGCCTGCAGCCATTCTGGACTAATTCAAGAATGCCAAGAATACTTCTCAAGATTGTGTCATGACAAACGGATTAAGCAAACTGTAGAGCATTATGCTTGCATGGTTGATCTCCTTGGTCGAGCTGGAAGATTAAAAGAAGCTAAGAATCTGATCGACAGCATGCTGCTAAAACCAAATATTGGAATATGGCAGACATTGCTTAGTGCTTGTAGAGTACATGGAGAGGTGGAGATAGGGAGAGAAGTGGGGGAGATGCTTCTGAGATTGGATGGCGATAACCCGGTCAACTATGTGATGCTGTCAAACATCTACGCAGAGGCCGGCTACTGGAAGGAGTGTGAGAGAATAAGAAAACTGTTTAAGAGGAAGGGGTTAAAGAAAGATGCAGGGCGGAGTTGGGTAGAGATTGACAAGGAGGTCTTCTTCTTCTACAATGGAGATAAGAGTCACCCACTTACAGAAAAGATTCATCAAGTATTGAAGGAAATGGAGAACAGAATAAAATCTGAGATCGGCTATGTTCATGGGGTGAGATTTGCACTGCATGACGTAGAGGAGGAGTCAAAAGAAGAGAGCTTGAGAGTTCATAGTGAAAAACTGGCAATCGGGTTGGCATTGGTATGTGGTGAGCTTGAGAAAGGGAATAGAACAATTCGGATCTTCAAGAACTTGAGGGTATGTGGTGATTGCCATGCCTTCATTAAAGGCTTGTCAAAGGTTTTGAAGGTGGTAATTCTTGTGAGAGATGCTAATAGGTTTCACAAGTTTGAGGACGGCGTGTGTTCTTGTGGAGATTATTGGTGA

Protein Analysis

686

Amino Acids

76.98

Weight (kDa)

8.21

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 68 - 113 4.6e-11 PPR repeat family
PPR_2 PF13041 169 - 216 1.9e-11 PPR repeat family
PPR PF01535 172 - 201 2.8e-07 PPR repeat
PPR_2 PF13041 374 - 422 5.8e-12 PPR repeat family
PPR PF01535 377 - 405 1.3e-06 PPR repeat
E_motif PF20431 492 - 554 3.5e-18 E motif
DYW_deaminase PF14432 591 - 686 2.5e-32 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 728
AasI GACNNNNNNGTC 1 cut(s) 1673
Acc36I ACCTGC 1 cut(s) 728
AccB7I CCANNNNNTGG 1 cut(s) 1436
AccI GTMKAC 1 cut(s) 620
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 3 cut(s) 34, 1214, 1648
AclWI GGATC 2 cut(s) 356, 1922
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 801
AcvI CACGTG 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 341
AfaI GTAC 2 cut(s) 317, 1473
AfiI CCNNNNNNNGG 2 cut(s) 1436, 1584
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 7 cut(s) 551, 809, 1198, 1283, 1738, 1921, 1981
AjnI CCWGG 3 cut(s) 215, 479, 879
Alw21I GWGCWC 3 cut(s) 175, 745, 1254
Alw44I GTGCAC 1 cut(s) 171
AlwI GGATC 2 cut(s) 356, 1922
AlwNI CAGNNNCTG 1 cut(s) 880
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 3 cut(s) 652, 876, 1575
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 4 cut(s) 416, 895, 1263, 1420
ApoI RAATTY 1 cut(s) 49
Asp700I GAANNNNTTC 1 cut(s) 1722
AspS9I GGNCC 1 cut(s) 877
AsuC2I CCSGG 3 cut(s) 346, 1153, 1538
AsuHPI GGTGA 7 cut(s) 436, 997, 1141, 1697, 1798, 1895, 1952
BaeGI GKGCMC 2 cut(s) 175, 838
BbrPI CACGTG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 1669
Bbv12I GWGCWC 3 cut(s) 175, 745, 1254
BbvI GCAGC 4 cut(s) 403, 907, 1275, 1407
BccI CCATC 4 cut(s) 920, 1027, 1223, 1520
BceAI ACGGC 1 cut(s) 2047
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 3 cut(s) 217, 481, 881
BclI TGATCA 3 cut(s) 696, 799, 1146
BcnI CCSGG 3 cut(s) 346, 1153, 1538
BfaI CTAG 3 cut(s) 906, 974, 1172
BfmI CTRYAG 3 cut(s) 417, 1261, 1338
BfuAI ACCTGC 1 cut(s) 728
BisI GCNGC 4 cut(s) 417, 896, 1264, 1421
BlsI GCNGC 4 cut(s) 418, 897, 1265, 1422
Bme1390I CCNGG 6 cut(s) 217, 346, 481, 881, 1153, 1538
BmgT120I GGNCC 1 cut(s) 877
BmrFI CCNGG 6 cut(s) 217, 346, 481, 881, 1153, 1538
BmsI GCATC 7 cut(s) 108, 530, 1198, 1500, 1542, 1630, 1993
BpiI GAAGAC 1 cut(s) 1669
BpmI CTGGAG 1 cut(s) 902
BpuEI CTTGAG 7 cut(s) 320, 623, 1052, 1288, 1855, 1910, 1948
BpuMI CCSGG 3 cut(s) 346, 1153, 1538
BsaAI YACGTR 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 1062
BsaHI GRCGYC 1 cut(s) 341
BsaJI CCNNGG 3 cut(s) 655, 879, 1369
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 4 cut(s) 160, 190, 554, 584
Bsc4I CCNNNNNNNGG 2 cut(s) 1436, 1584
Bse118I RCCGGY 2 cut(s) 499, 1577
Bse1I ACTGG 2 cut(s) 1589, 1860
Bse3DI GCAATG 5 cut(s) 259, 436, 534, 732, 1451
Bse8I GATNNNNATC 1 cut(s) 1062
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 3 cut(s) 217, 481, 881
BseDI CCNNGG 3 cut(s) 655, 879, 1369
BseGI GGATG 4 cut(s) 489, 545, 619, 1531
BseJI GATNNNNATC 1 cut(s) 1062
BseLI CCNNNNNNNGG 2 cut(s) 1436, 1584
BseMI GCAATG 5 cut(s) 259, 436, 534, 732, 1451
BseMII CTCAG 6 cut(s) 189, 800, 913, 1127, 1508, 1755
BseNI ACTGG 2 cut(s) 1589, 1860
BseRI GAGGAG 2 cut(s) 1826, 1829
BseSI GKGCMC 2 cut(s) 175, 838
BseXI GCAGC 4 cut(s) 403, 907, 1275, 1407
BseYI CCCAGC 1 cut(s) 598
BsgI GTGCAG 1 cut(s) 787
BshFI GGCC 3 cut(s) 654, 878, 1577
BsiHKAI GWGCWC 3 cut(s) 175, 745, 1254
BsiSI CCGG 6 cut(s) 345, 500, 611, 1152, 1538, 1578
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 2 cut(s) 1436, 1584
BsmFI GGGAC 1 cut(s) 114
BsmI GAATGC 4 cut(s) 198, 378, 866, 1292
BsnI GGCC 3 cut(s) 654, 878, 1577
Bsp1286I GDGCHC 4 cut(s) 175, 745, 838, 1254
Bsp13I TCCGGA 1 cut(s) 610
BspACI CCGC 3 cut(s) 34, 1214, 1648
BspANI GGCC 3 cut(s) 654, 878, 1577
BspCNI CTCAG 6 cut(s) 190, 799, 912, 1126, 1509, 1756
BspEI TCCGGA 1 cut(s) 610
BspHI TCATGA 1 cut(s) 1315
BspMAI CTGCAG 2 cut(s) 421, 1265
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 2 cut(s) 356, 1922
BsrDI GCAATG 5 cut(s) 259, 436, 534, 732, 1451
BsrFI RCCGGY 2 cut(s) 499, 1577
BsrI ACTGG 2 cut(s) 1589, 1860
BssAI RCCGGY 2 cut(s) 499, 1577
BssECI CCNNGG 3 cut(s) 655, 879, 1369
BssNAI GTATAC 1 cut(s) 621
BssNI GRCGYC 1 cut(s) 341
BssT1I CCWWGG 2 cut(s) 655, 1369
Bst1107I GTATAC 1 cut(s) 621
Bst2UI CCWGG 3 cut(s) 217, 481, 881
Bst4CI ACNGT 6 cut(s) 41, 248, 386, 547, 1339, 1614
Bst6I CTCTTC 2 cut(s) 457, 1821
BstACI GRCGYC 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 1453
BstBAI YACGTR 1 cut(s) 176
BstC8I GCNNGC 8 cut(s) 75, 501, 770, 999, 1261, 1354, 1418, 1579
BstF5I GGATG 4 cut(s) 489, 545, 619, 1531
BstMWI GCNNNNNNNGC 3 cut(s) 533, 968, 1453
BstNI CCWGG 3 cut(s) 217, 481, 881
BstNSI RCATGY 3 cut(s) 448, 1001, 1420
BstSCI CCNGG 6 cut(s) 215, 344, 479, 879, 1151, 1536
BstSFI CTRYAG 3 cut(s) 417, 1261, 1338
BstSLI GKGCMC 2 cut(s) 175, 838
BstV1I GCAGC 4 cut(s) 403, 907, 1275, 1407
BstV2I GAAGAC 1 cut(s) 1669
BstX2I RGATCY 2 cut(s) 348, 1914
BstYI RGATCY 2 cut(s) 348, 1914
BstZ17I GTATAC 1 cut(s) 621
BsuRI GGCC 3 cut(s) 654, 878, 1577
BtsCI GGATG 4 cut(s) 489, 545, 619, 1531
BtsI GCAGTG 3 cut(s) 426, 1254, 1796
BtsIMutI CAGTG 7 cut(s) 93, 253, 286, 426, 496, 1254, 1796
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 8 cut(s) 75, 501, 770, 999, 1261, 1354, 1418, 1579
CaiI CAGNNNCTG 1 cut(s) 880
CciI TCATGA 1 cut(s) 1315
Cfr10I RCCGGY 2 cut(s) 499, 1577
Cfr13I GGNCC 1 cut(s) 877
CseI GACGC 2 cut(s) 330, 465
Csp6I GTAC 2 cut(s) 316, 1472
CviQI GTAC 2 cut(s) 316, 1472
DrdI GACNNNNNNGTC 1 cut(s) 1673
DseDI GACNNNNNNGTC 1 cut(s) 1673
Eam1104I CTCTTC 2 cut(s) 457, 1821
EarI CTCTTC 2 cut(s) 457, 1821
EciI GGCGGA 2 cut(s) 1229, 1663
Eco130I CCWWGG 2 cut(s) 655, 1369
Eco57I CTGAAG 1 cut(s) 801
Eco72I CACGTG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 877
EcoRII CCWGG 3 cut(s) 215, 479, 879
EcoT14I CCWWGG 2 cut(s) 655, 1369
EcoT22I ATGCAT 1 cut(s) 999
ErhI CCWWGG 2 cut(s) 655, 1369
FalI AAGNNNNNCTT 2 cut(s) 1661, 1693
FaqI GGGAC 1 cut(s) 114
FbaI TGATCA 3 cut(s) 696, 799, 1146
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 4 cut(s) 417, 896, 1264, 1421
FokI GGATG 4 cut(s) 496, 552, 626, 1538
Fsp4HI GCNGC 4 cut(s) 417, 896, 1264, 1421
FspBI CTAG 3 cut(s) 906, 974, 1172
GluI GCNGC 4 cut(s) 417, 896, 1264, 1421
GsaI CCCAGC 1 cut(s) 602
GsuI CTGGAG 1 cut(s) 902
HaeIII GGCC 3 cut(s) 654, 878, 1577
HapII CCGG 6 cut(s) 345, 500, 611, 1152, 1538, 1578
HgaI GACGC 2 cut(s) 330, 465
Hin1I GRCGYC 1 cut(s) 341
HincII GTYRAC 1 cut(s) 1543
HindII GTYRAC 1 cut(s) 1543
HinfI GANTC 8 cut(s) 25, 817, 841, 1051, 1402, 1702, 1723, 1817
HpaII CCGG 6 cut(s) 345, 500, 611, 1152, 1538, 1578
HphI GGTGA 7 cut(s) 436, 997, 1141, 1697, 1798, 1895, 1952
Hpy166II GTNNAC 4 cut(s) 173, 318, 621, 1543
Hpy188I TCNGA 7 cut(s) 726, 789, 902, 1407, 1518, 1765, 1914
Hpy8I GTNNAC 4 cut(s) 173, 318, 621, 1543
HpyAV CCTTC 6 cut(s) 545, 1582, 1618, 1732, 1963, 1975
HpyCH4III ACNGT 6 cut(s) 41, 248, 386, 547, 1339, 1614
HpyCH4IV ACGT 2 cut(s) 175, 1806
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 968, 1453
HpySE526I ACGT 2 cut(s) 175, 1806
Hsp92I GRCGYC 1 cut(s) 341
Kpn2I TCCGGA 1 cut(s) 610
KroI GCCGGC 2 cut(s) 499, 1577
KroNI GCCGGC 2 cut(s) 501, 1579
Ksp22I TGATCA 3 cut(s) 696, 799, 1146
LmnI GCTCC 1 cut(s) 659
Lsp1109I GCAGC 4 cut(s) 403, 907, 1275, 1407
LweI GCATC 7 cut(s) 108, 530, 1198, 1500, 1542, 1630, 1993
MaeI CTAG 3 cut(s) 906, 974, 1172
MaeII ACGT 2 cut(s) 175, 1806
MaeIII GTNAC 2 cut(s) 1234, 1703
MboII GAAGA 9 cut(s) 474, 826, 1189, 1395, 1669, 1672, 1675, 1838, 1909
MflI RGATCY 2 cut(s) 348, 1914
MhlI GDGCHC 4 cut(s) 175, 745, 838, 1254
MluCI AATT 8 cut(s) 49, 663, 865, 1128, 1140, 1278, 1908, 1989
MlyI GAGTC 2 cut(s) 1711, 1826
Mph1103I ATGCAT 1 cut(s) 999
MroI TCCGGA 1 cut(s) 610
MroNI GCCGGC 2 cut(s) 499, 1577
MroXI GAANNNNTTC 1 cut(s) 1722
MslI CAYNNNNRTG 3 cut(s) 449, 1037, 1876
MspI CCGG 6 cut(s) 345, 500, 611, 1152, 1538, 1578
MspR9I CCNGG 6 cut(s) 217, 346, 481, 881, 1153, 1538
Mva1269I GAATGC 4 cut(s) 198, 378, 866, 1292
MvaI CCWGG 3 cut(s) 217, 481, 881
MwoI GCNNNNNNNGC 3 cut(s) 533, 968, 1453
NaeI GCCGGC 2 cut(s) 501, 1579
NciI CCSGG 3 cut(s) 346, 1153, 1538
NgoMIV GCCGGC 2 cut(s) 499, 1577
NmuCI GTSAC 2 cut(s) 1234, 1703
NsiI ATGCAT 1 cut(s) 999
NspI RCATGY 3 cut(s) 448, 1001, 1420
PaeI GCATGC 2 cut(s) 1001, 1420
PagI TCATGA 1 cut(s) 1315
PaqCI CACCTGC 1 cut(s) 728
PciI ACATGT 1 cut(s) 444
PctI GAATGC 4 cut(s) 198, 378, 866, 1292
PdiI GCCGGC 2 cut(s) 501, 1579
PdmI GAANNNNTTC 1 cut(s) 1722
PfeI GAWTC 6 cut(s) 25, 817, 841, 1051, 1402, 1723
PflMI CCANNNNNTGG 1 cut(s) 1436
PfoI TCCNGGA 3 cut(s) 215, 344, 479
PkrI GCNGC 4 cut(s) 418, 897, 1265, 1422
PleI GAGTC 2 cut(s) 1710, 1825
PmaCI CACGTG 1 cut(s) 176
PmlI CACGTG 1 cut(s) 176
PpsI GAGTC 2 cut(s) 1710, 1825
Ppu21I YACGTR 1 cut(s) 176
PscI ACATGT 1 cut(s) 444
Psp6I CCWGG 3 cut(s) 215, 479, 879
PspCI CACGTG 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 598
PspGI CCWGG 3 cut(s) 215, 479, 879
PspPI GGNCC 1 cut(s) 877
PstI CTGCAG 2 cut(s) 421, 1265
PstNI CAGNNNCTG 1 cut(s) 880
PsuI RGATCY 2 cut(s) 348, 1914
RsaI GTAC 2 cut(s) 317, 1473
RsaNI GTAC 2 cut(s) 316, 1472
RseI CAYNNNNRTG 3 cut(s) 449, 1037, 1876
SatI GCNGC 4 cut(s) 417, 896, 1264, 1421
Sau96I GGNCC 1 cut(s) 877
SchI GAGTC 2 cut(s) 1711, 1826
ScrFI CCNGG 6 cut(s) 217, 346, 481, 881, 1153, 1538
SduI GDGCHC 4 cut(s) 175, 745, 838, 1254
SfaNI GCATC 7 cut(s) 108, 530, 1198, 1500, 1542, 1630, 1993
SfcI CTRYAG 3 cut(s) 417, 1261, 1338
SmiMI CAYNNNNRTG 3 cut(s) 449, 1037, 1876
SmlI CTYRAG 7 cut(s) 335, 638, 1067, 1303, 1834, 1889, 1927
SmoI CTYRAG 7 cut(s) 335, 638, 1067, 1303, 1834, 1889, 1927
SphI GCATGC 2 cut(s) 1001, 1420
Sse9I AATT 8 cut(s) 49, 663, 865, 1128, 1140, 1278, 1908, 1989
SsiI CCGC 3 cut(s) 34, 1214, 1648
SspI AATATT 1 cut(s) 1435
SspMI CTAG 3 cut(s) 906, 974, 1172
StyD4I CCNGG 6 cut(s) 215, 344, 479, 879, 1151, 1536
StyI CCWWGG 2 cut(s) 655, 1369
TaaI ACNGT 6 cut(s) 41, 248, 386, 547, 1339, 1614
TaiI ACGT 2 cut(s) 178, 1809
TaqI TCGA 5 cut(s) 326, 941, 1049, 1375, 1410
TasI AATT 8 cut(s) 49, 663, 865, 1128, 1140, 1278, 1908, 1989
TatI WGTACW 2 cut(s) 315, 1471
TfiI GAWTC 6 cut(s) 25, 817, 841, 1051, 1402, 1723
TscAI CASTG 7 cut(s) 93, 253, 286, 426, 496, 1254, 1803
TseFI GTSAC 2 cut(s) 1234, 1703
TseI GCWGC 4 cut(s) 416, 895, 1263, 1420
Tsp45I GTSAC 2 cut(s) 1234, 1703
TspDTI ATGAA 8 cut(s) 17, 423, 1101, 1122, 1715, 1769, 1832, 1945
TspGWI ACGGA 1 cut(s) 1339
TspRI CASTG 7 cut(s) 93, 253, 286, 426, 496, 1254, 1803
Van91I CCANNNNNTGG 1 cut(s) 1436
VneI GTGCAC 1 cut(s) 171
XapI RAATTY 1 cut(s) 49
XceI RCATGY 3 cut(s) 448, 1001, 1420
XmiI GTMKAC 1 cut(s) 620
XmnI GAANNNNTTC 1 cut(s) 1722
XspI CTAG 3 cut(s) 906, 974, 1172
Zsp2I ATGCAT 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.