Rh6DG454100

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011061
Physical Location & Seq
Forward (+)
62557148 .. 62558923
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG454100.1

Sequence Viewer

Length: 1776 bp
ATGGGATTTTCGGGAGTTAAGCCGAATGAGTACACTCTTTCGACTAATCTCAAGGCGTCTGGGATCTTGGGGATTGCTGATAATGGAATGCAGATACACAGTATGTGCGCTAAGTCTGGTTATGAATGGGCTGCAGTGGTGAGCAATGCTATCCTTGACATGTATGCGAAATGTGGAAGAGTTAGTGAAGCGTCCAGGATGTTCAGTGTTATGCCGGCTAAGAATGTTATTACTTGGAACACAATGATAGCTGGATACACGGTTGAAGGAAATGGGGAGAAAGCGTTGGTATTGTTTAGGAAAATGCAAGAGCTGGGAGAAGTTCCGGATGAGTATACGATTACAAGCACACTCAAGGCTTGTAGTGGCCTTGGAGCAATTCGGCAAGGAAGCCAAATACATTCCTCCTTGATCACAAGGGGGTTTCTGTGTTCAGTTCGGACGATTATTGCAGGTGCTCTCGTTGATATGTATGTGAAGTGCAGGCACTTAACTGAAGCTCAGAGAGTGTTTGATCAAGTTGAACAGAAGAATCTAATATCTTGGAGTGCCCTGATTCTTGGTTATGCCCAAGAATGCAATTTAACACAGGCCATGGAGTTGTTTAGGCAGCTCAGAGCTAGCGTATGTCATCAAGTAGATGGGTTTGTTCTGTCGAGCCTTATGGGTGTGTTTGCTGATTTTGCTCTAGTAGAACAAGGTAGGCAAATGCATGCTTTCACCACTAAAATCCCATCTGGTTTAGACATATCGGTGTTTAACTCGATTCTGGATATGTATCTCAAGTGTGGTTTGACAGATGAGGCAGAAAGACTTTTCGATGAAATTCAGTTGAGAAATGTAATTTCTTGGACAATTATGATCACCGGGTTTGGAAAGCATGGTCTGGGGAAGAAAGCTGTGCTTCTTTTCAACCAAATGCAATCGGAGGAAATAGAACCTGATGGAGTGACTTACTTGGCAGTGCTCTCAGCCTGCAGCCATTCTGGACTAATTCAAGAATGCCAAGAATACTTCTCAAGATTGTGTCATGACAAACGGATTAAGCAAACTGTAGAGCATTATGCTTGCATGGTTGATCTCCTTGGTCGAGCAGGAAGATTAAAAGAAGCTAAGAATCTGATTGACAGCATGCTGCTAAAACCAAATATTGGAATATGGCAGACATTGCTTAGTGCTTGTAGAGTACATGGAGAGGTGGAGATAGGGAGAGAAGTGGGAGAGATGCTTCTGAGGTTGGATGGTGATAACGCGGTCAACTATGTGATGCTGTCAAACCTCTATGCAGAGGCCGGCTACTGGAAGGAGTGTGAGAGAATAAGAAAACTGTTTAAGAGGAAGGGGTTAAAAAAAGATGCAGGACGGAGTTGGGTAGAGATTGACAAGGAGGTCTACTTCTTCTACAATGGAGATGAGAGTCATCCACTTACAGAAAAGATTCATCAAGTATTGAAGGAAATGGAGAACAGAATAAAATCTGAGATCGGCTACGTTCATGGGGTGAGATTTGCACTGCATGACGTAGAAGAGGAGTCAAAAGAAGAGAGCTTGAGAGTTCATAGTGAAAAACTGGCAATCGGGTTGGCATTGGTATGTGGTGAGCTTGAGAAAGGGAATAGAACAATTCGGATCTTCAAGAACTTGAGGGTATGTGGTGATTGCCATGCCTTCATTAAAGGCTTGTCAAAGGTTTTGAAGGTGGTAATTCTTGTGAGAGATGCTAATAGGTTTCACAAGTTTGAGGACGGCGTGTGTTCTTGTGGAGATTATTGGTGA

Protein Analysis

591

Amino Acids

66.38

Weight (kDa)

6.98

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 74 - 121 1.9e-12 PPR repeat family
PPR PF01535 77 - 106 6e-08 PPR repeat
PPR_2 PF13041 279 - 327 1.2e-11 PPR repeat family
PPR PF01535 282 - 310 9.8e-07 PPR repeat
E_motif PF20431 397 - 459 5.2e-17 E motif
DYW_deaminase PF14432 496 - 591 2e-32 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 443
AasI GACNNNNNNGTC 1 cut(s) 1388
Acc36I ACCTGC 1 cut(s) 443
AccB7I CCANNNNNTGG 1 cut(s) 1151
AccI GTMKAC 2 cut(s) 335, 1392
AccII CGCG 1 cut(s) 1253
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 1 cut(s) 1253
AclWI GGATC 2 cut(s) 71, 1637
AcsI RAATTY 1 cut(s) 825
AcuI CTGAAG 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 2 cut(s) 32, 1188
AfiI CCNNNNNNNGG 2 cut(s) 1151, 1299
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 7 cut(s) 266, 524, 913, 998, 1453, 1636, 1696
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 9 cut(s) 251, 313, 500, 613, 620, 899, 1112, 1548, 1603
AluI AGCT 9 cut(s) 251, 313, 500, 613, 620, 899, 1112, 1548, 1603
Alw21I GWGCWC 2 cut(s) 460, 969
AlwI GGATC 2 cut(s) 71, 1637
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 3 cut(s) 367, 591, 1290
ApeKI GCWGC 4 cut(s) 131, 610, 978, 1135
ApoI RAATTY 1 cut(s) 825
Asp700I GAANNNNTTC 1 cut(s) 1437
AspLEI GCGC 1 cut(s) 110
AsuC2I CCSGG 1 cut(s) 868
AsuHPI GGTGA 7 cut(s) 151, 712, 856, 1256, 1513, 1610, 1667
AsuNHI GCTAGC 1 cut(s) 620
BaeGI GKGCMC 1 cut(s) 553
Bbv12I GWGCWC 2 cut(s) 460, 969
BbvI GCAGC 4 cut(s) 118, 622, 990, 1122
BccI CCATC 4 cut(s) 635, 742, 938, 1235
BceAI ACGGC 1 cut(s) 1762
BciT130I CCWGG 1 cut(s) 196
BciVI GTATCC 1 cut(s) 248
BclI TGATCA 3 cut(s) 411, 514, 861
BcnI CCSGG 1 cut(s) 868
BfaI CTAG 2 cut(s) 621, 689
BfmI CTRYAG 3 cut(s) 132, 976, 1053
BfuAI ACCTGC 1 cut(s) 443
BfuI GTATCC 1 cut(s) 248
BisI GCNGC 4 cut(s) 132, 611, 979, 1136
BlsI GCNGC 4 cut(s) 133, 612, 980, 1137
Bme1390I CCNGG 2 cut(s) 196, 868
BmrFI CCNGG 2 cut(s) 196, 868
BmsI GCATC 4 cut(s) 1215, 1257, 1345, 1708
BmtI GCTAGC 1 cut(s) 624
BpuEI CTTGAG 7 cut(s) 35, 338, 767, 1003, 1570, 1625, 1663
BpuMI CCSGG 1 cut(s) 868
BsaBI GATNNNNATC 1 cut(s) 777
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 3 cut(s) 370, 594, 1084
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 1151, 1299
Bse118I RCCGGY 2 cut(s) 214, 1292
Bse1I ACTGG 2 cut(s) 1304, 1575
Bse3DI GCAATG 2 cut(s) 151, 1166
Bse8I GATNNNNATC 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 3 cut(s) 370, 594, 1084
BseGI GGATG 4 cut(s) 204, 334, 1246, 1420
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 2 cut(s) 1151, 1299
BseMI GCAATG 2 cut(s) 151, 1166
BseMII CTCAG 5 cut(s) 515, 628, 984, 1223, 1470
BseNI ACTGG 2 cut(s) 1304, 1575
BseRI GAGGAG 1 cut(s) 1544
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 4 cut(s) 118, 622, 990, 1122
BseYI CCCAGC 1 cut(s) 313
BsgI GTGCAG 1 cut(s) 502
Bsh1236I CGCG 1 cut(s) 1253
BshFI GGCC 3 cut(s) 369, 593, 1292
BsiHKAI GWGCWC 2 cut(s) 460, 969
BsiSI CCGG 4 cut(s) 215, 326, 867, 1293
BslI CCNNNNNNNGG 2 cut(s) 1151, 1299
BsmI GAATGC 3 cut(s) 93, 581, 1007
BsnI GGCC 3 cut(s) 369, 593, 1292
Bsp1286I GDGCHC 3 cut(s) 460, 553, 969
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 7 cut(s) 63, 411, 514, 861, 1078, 1482, 1629
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 1 cut(s) 1253
BspANI GGCC 3 cut(s) 369, 593, 1292
BspCNI CTCAG 5 cut(s) 514, 627, 983, 1224, 1471
BspEI TCCGGA 1 cut(s) 325
BspFNI CGCG 1 cut(s) 1253
BspHI TCATGA 1 cut(s) 1030
BspMAI CTGCAG 2 cut(s) 136, 980
BspMI ACCTGC 1 cut(s) 443
BspOI GCTAGC 1 cut(s) 624
BspPI GGATC 2 cut(s) 71, 1637
BsrDI GCAATG 2 cut(s) 151, 1166
BsrFI RCCGGY 2 cut(s) 214, 1292
BsrI ACTGG 2 cut(s) 1304, 1575
BssAI RCCGGY 2 cut(s) 214, 1292
BssECI CCNNGG 3 cut(s) 370, 594, 1084
BssMI GATC 7 cut(s) 63, 411, 514, 861, 1078, 1482, 1629
BssNAI GTATAC 1 cut(s) 336
BssNI GRCGYC 1 cut(s) 56
BssT1I CCWWGG 3 cut(s) 370, 594, 1084
Bst1107I GTATAC 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 4 cut(s) 101, 262, 1054, 1329
Bst6I CTCTTC 3 cut(s) 172, 1521, 1536
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 1168
BstC8I GCNNGC 8 cut(s) 216, 485, 622, 714, 976, 1069, 1133, 1294
BstDEI CTNAG 9 cut(s) 111, 219, 501, 614, 970, 1113, 1172, 1232, 1479
BstDSI CCRYGG 1 cut(s) 594
BstF5I GGATG 4 cut(s) 204, 334, 1246, 1420
BstFNI CGCG 1 cut(s) 1253
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 7 cut(s) 66, 414, 517, 864, 1081, 1485, 1632
BstMBI GATC 7 cut(s) 63, 411, 514, 861, 1078, 1482, 1629
BstMWI GCNNNNNNNGC 2 cut(s) 683, 1168
BstNI CCWGG 1 cut(s) 196
BstNSI RCATGY 3 cut(s) 163, 716, 1135
BstSCI CCNGG 2 cut(s) 194, 866
BstSFI CTRYAG 3 cut(s) 132, 976, 1053
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 1 cut(s) 1253
BstV1I GCAGC 4 cut(s) 118, 622, 990, 1122
BstX2I RGATCY 2 cut(s) 63, 1629
BstYI RGATCY 2 cut(s) 63, 1629
BstZ17I GTATAC 1 cut(s) 336
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 3 cut(s) 369, 593, 1292
BtgI CCRYGG 1 cut(s) 594
BtsCI GGATG 4 cut(s) 204, 334, 1246, 1420
BtsI GCAGTG 3 cut(s) 141, 969, 1511
BtsIMutI CAGTG 4 cut(s) 141, 211, 969, 1511
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 8 cut(s) 216, 485, 622, 714, 976, 1069, 1133, 1294
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 1 cut(s) 110
Cfr10I RCCGGY 2 cut(s) 214, 1292
CseI GACGC 2 cut(s) 45, 180
Csp6I GTAC 2 cut(s) 31, 1187
CviQI GTAC 2 cut(s) 31, 1187
DdeI CTNAG 9 cut(s) 111, 219, 501, 614, 970, 1113, 1172, 1232, 1479
DpnI GATC 7 cut(s) 65, 413, 516, 863, 1080, 1484, 1631
DpnII GATC 7 cut(s) 63, 411, 514, 861, 1078, 1482, 1629
DrdI GACNNNNNNGTC 1 cut(s) 1388
DseDI GACNNNNNNGTC 1 cut(s) 1388
Eam1104I CTCTTC 3 cut(s) 172, 1521, 1536
EarI CTCTTC 3 cut(s) 172, 1521, 1536
Eco130I CCWWGG 3 cut(s) 370, 594, 1084
Eco57I CTGAAG 1 cut(s) 516
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 3 cut(s) 370, 594, 1084
EcoT22I ATGCAT 1 cut(s) 714
ErhI CCWWGG 3 cut(s) 370, 594, 1084
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FbaI TGATCA 3 cut(s) 411, 514, 861
FblI GTMKAC 2 cut(s) 335, 1392
Fnu4HI GCNGC 4 cut(s) 132, 611, 979, 1136
FokI GGATG 4 cut(s) 211, 341, 1253, 1407
Fsp4HI GCNGC 4 cut(s) 132, 611, 979, 1136
FspBI CTAG 2 cut(s) 621, 689
GlaI GCGC 1 cut(s) 109
GluI GCNGC 4 cut(s) 132, 611, 979, 1136
GsaI CCCAGC 1 cut(s) 317
HaeIII GGCC 3 cut(s) 369, 593, 1292
HapII CCGG 4 cut(s) 215, 326, 867, 1293
HgaI GACGC 2 cut(s) 45, 180
HhaI GCGC 1 cut(s) 110
Hin1I GRCGYC 1 cut(s) 56
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HincII GTYRAC 1 cut(s) 1258
HindII GTYRAC 1 cut(s) 1258
HinfI GANTC 7 cut(s) 532, 556, 766, 1117, 1417, 1438, 1532
HpaII CCGG 4 cut(s) 215, 326, 867, 1293
HphI GGTGA 7 cut(s) 151, 712, 856, 1256, 1513, 1610, 1667
Hpy166II GTNNAC 4 cut(s) 33, 336, 1258, 1393
Hpy188I TCNGA 8 cut(s) 441, 504, 617, 928, 1122, 1233, 1480, 1629
Hpy188III TCNNGA 8 cut(s) 12, 326, 770, 987, 998, 1020, 1031, 1636
Hpy8I GTNNAC 4 cut(s) 33, 336, 1258, 1393
HpyAV CCTTC 6 cut(s) 260, 1297, 1333, 1447, 1678, 1690
HpyCH4III ACNGT 4 cut(s) 101, 262, 1054, 1329
HpyCH4IV ACGT 2 cut(s) 1491, 1521
HpyF10VI GCNNNNNNNGC 2 cut(s) 683, 1168
HpyF3I CTNAG 9 cut(s) 111, 219, 501, 614, 970, 1113, 1172, 1232, 1479
HpySE526I ACGT 2 cut(s) 1491, 1521
Hsp92I GRCGYC 1 cut(s) 56
HspAI GCGC 1 cut(s) 108
Kpn2I TCCGGA 1 cut(s) 325
KroI GCCGGC 2 cut(s) 214, 1292
KroNI GCCGGC 2 cut(s) 216, 1294
Ksp22I TGATCA 3 cut(s) 411, 514, 861
Kzo9I GATC 7 cut(s) 63, 411, 514, 861, 1078, 1482, 1629
LmnI GCTCC 1 cut(s) 374
Lsp1109I GCAGC 4 cut(s) 118, 622, 990, 1122
LweI GCATC 4 cut(s) 1215, 1257, 1345, 1708
MaeI CTAG 2 cut(s) 621, 689
MaeII ACGT 2 cut(s) 1491, 1521
MaeIII GTNAC 1 cut(s) 949
MalI GATC 7 cut(s) 65, 413, 516, 863, 1080, 1484, 1631
MboI GATC 7 cut(s) 63, 411, 514, 861, 1078, 1482, 1629
MboII GAAGA 8 cut(s) 189, 541, 904, 1110, 1390, 1538, 1553, 1624
MflI RGATCY 2 cut(s) 63, 1629
MhlI GDGCHC 3 cut(s) 460, 553, 969
MluCI AATT 8 cut(s) 378, 580, 825, 843, 855, 993, 1623, 1704
MlyI GAGTC 2 cut(s) 1426, 1541
MmeI TCCRAC 1 cut(s) 1218
Mph1103I ATGCAT 1 cut(s) 714
MroI TCCGGA 1 cut(s) 325
MroNI GCCGGC 2 cut(s) 214, 1292
MroXI GAANNNNTTC 1 cut(s) 1437
MseI TTAA 9 cut(s) 18, 491, 584, 759, 1044, 1103, 1332, 1346, 1674
MslI CAYNNNNRTG 2 cut(s) 752, 1591
MspI CCGG 4 cut(s) 215, 326, 867, 1293
MspR9I CCNGG 2 cut(s) 196, 868
Mva1269I GAATGC 3 cut(s) 93, 581, 1007
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 1 cut(s) 1253
MwoI GCNNNNNNNGC 2 cut(s) 683, 1168
NaeI GCCGGC 2 cut(s) 216, 1294
NciI CCSGG 1 cut(s) 868
NcoI CCATGG 1 cut(s) 594
NdeII GATC 7 cut(s) 63, 411, 514, 861, 1078, 1482, 1629
NgoMIV GCCGGC 2 cut(s) 214, 1292
NheI GCTAGC 1 cut(s) 620
NmuCI GTSAC 1 cut(s) 949
NsiI ATGCAT 1 cut(s) 714
NspI RCATGY 3 cut(s) 163, 716, 1135
PaeI GCATGC 2 cut(s) 716, 1135
PagI TCATGA 1 cut(s) 1030
PaqCI CACCTGC 1 cut(s) 443
PciI ACATGT 1 cut(s) 159
PctI GAATGC 3 cut(s) 93, 581, 1007
PdiI GCCGGC 2 cut(s) 216, 1294
PdmI GAANNNNTTC 1 cut(s) 1437
PfeI GAWTC 5 cut(s) 532, 556, 766, 1117, 1438
PflMI CCANNNNNTGG 1 cut(s) 1151
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 4 cut(s) 133, 612, 980, 1137
PleI GAGTC 2 cut(s) 1425, 1540
PpsI GAGTC 2 cut(s) 1425, 1540
PscI ACATGT 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 194
PspFI CCCAGC 1 cut(s) 313
PspGI CCWGG 1 cut(s) 194
PstI CTGCAG 2 cut(s) 136, 980
PsuI RGATCY 2 cut(s) 63, 1629
RsaI GTAC 2 cut(s) 32, 1188
RsaNI GTAC 2 cut(s) 31, 1187
RseI CAYNNNNRTG 2 cut(s) 752, 1591
SaqAI TTAA 9 cut(s) 18, 491, 584, 759, 1044, 1103, 1332, 1346, 1674
SatI GCNGC 4 cut(s) 132, 611, 979, 1136
Sau3AI GATC 7 cut(s) 63, 411, 514, 861, 1078, 1482, 1629
SchI GAGTC 2 cut(s) 1426, 1541
ScrFI CCNGG 2 cut(s) 196, 868
SduI GDGCHC 3 cut(s) 460, 553, 969
SfaNI GCATC 4 cut(s) 1215, 1257, 1345, 1708
SfcI CTRYAG 3 cut(s) 132, 976, 1053
SmiMI CAYNNNNRTG 2 cut(s) 752, 1591
SmlI CTYRAG 7 cut(s) 50, 353, 782, 1018, 1549, 1604, 1642
SmoI CTYRAG 7 cut(s) 50, 353, 782, 1018, 1549, 1604, 1642
SphI GCATGC 2 cut(s) 716, 1135
Sse9I AATT 8 cut(s) 378, 580, 825, 843, 855, 993, 1623, 1704
SsiI CCGC 1 cut(s) 1253
SspI AATATT 1 cut(s) 1150
SspMI CTAG 2 cut(s) 621, 689
StyD4I CCNGG 2 cut(s) 194, 866
StyI CCWWGG 3 cut(s) 370, 594, 1084
TaaI ACNGT 4 cut(s) 101, 262, 1054, 1329
TaiI ACGT 2 cut(s) 1494, 1524
TaqI TCGA 5 cut(s) 41, 656, 764, 819, 1090
TasI AATT 8 cut(s) 378, 580, 825, 843, 855, 993, 1623, 1704
TatI WGTACW 2 cut(s) 30, 1186
TfiI GAWTC 5 cut(s) 532, 556, 766, 1117, 1438
Tru1I TTAA 9 cut(s) 18, 491, 584, 759, 1044, 1103, 1332, 1346, 1674
Tru9I TTAA 9 cut(s) 18, 491, 584, 759, 1044, 1103, 1332, 1346, 1674
TscAI CASTG 4 cut(s) 141, 211, 969, 1518
TseFI GTSAC 1 cut(s) 949
TseI GCWGC 4 cut(s) 131, 610, 978, 1135
Tsp45I GTSAC 1 cut(s) 949
TspDTI ATGAA 6 cut(s) 138, 837, 1430, 1484, 1547, 1660
TspGWI ACGGA 2 cut(s) 1054, 1378
TspRI CASTG 4 cut(s) 141, 211, 969, 1518
Van91I CCANNNNNTGG 1 cut(s) 1151
XapI RAATTY 1 cut(s) 825
XceI RCATGY 3 cut(s) 163, 716, 1135
XmiI GTMKAC 2 cut(s) 335, 1392
XmnI GAANNNNTTC 1 cut(s) 1437
XspI CTAG 2 cut(s) 621, 689
Zsp2I ATGCAT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.