pycom15g09590

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006256
Physical Location & Seq
Reverse (-)
6244522 .. 6255071
10550 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3522 bp
ATGGCGATGATACATTCTAAACGGCTGTCCACCGCTTCCTCCTTCATTTTCTCAAACACCTTCGCTGCCTCCGCCCTGTGTCTCCGCCGCTCCATGGCCAAGCTGCAGTGGAGGGCGTTTGAGGCTTATCCATACGCTTGCTTACCATCTTCGTCAAATTTGACAACGAATGTATACAGAAATTATTCTCAGCATAGATTAAAAAAGTCCGGAGTTTCTTGCATGATCACGGCCAACCCTTCGAAGTTTGGGCCGAATTTTCATCCTTTCAGAAGAAGTGGCGATGGGTCTGAATGCGCTTTTACCTTTCATTCTCATTTAAAACATGGTTCGCTTAATTCTTTAGGGCTTGCTGGTATTTTCAGTTGTGGCTCTTTTAAGAATAGTTGCAGATTAGGTTTATCTAATCATGGTGCTGGAGCTTTTCCCAAGAGATTTTTCTCTCAAGTTCCATCTGTGATAAACAAAGGAGAGAGTGTGGGTGTCCAAGATGGCAGGAATAGTGGAAATATGTTTTTTTTCAGAAGGCTTATTAAATGCAAGAAATGGGAAAAGGCATTAGCAGCTCAAAGCAAAACCAAAGCTACTGATTCTAGTATCTCTAAAGATGTTGAAGTAAAAGCCTTAACCGAACAGAAAGTTGGAAGTGAGAAGGGGGCAGGAAAGTTGGGATCTTTGTTTTCCAATTCTCCTATAAGAAATGATACCCAGGTCTCTAAAGTCAAAGGGAAAAAGAGGTCGAAGAGCAAGAAAACTACAGAACAAATTTCTGCTGAAAATCCTCCAGCAGAAGCTGCTATGTCACAAAGTTCTAAGGTTACTTCTCAACCAAAGAAATCTGGTGGTACCAAAAGTGGCGAAAAGTCAGCGGAGGTTTCAGAGTTGAACCTCACTGCGAAAACTCCCGTGGAAATTGCTGATGGTTCTTCAACAAAACCCCAGCCAAAGAAGAAAAGTGGGAACTCTTTTAGAAGGGGAAAATTAATAAATTCAGATACCAAGTCGCCACAGAAACTTATACCACAAAAAATAGGCAGAGTGAAACCTCATGGGAAAATGACACTGAAGCAGCTGTACCCTGCAACTGGGAAAACTGTGGTGGTGGTGGAGTCTGCTACAAAGGCAAAGGTTATTCAGGGTTATCTTGGTGATATGTTTGAAGTTCTTCCTAGCTATGGTCATGTAAGGGACTTGGCTGCTAGGTCCGGATCTGTACGGCCTGATGATGATTTTAGTATGGTGTGGGAAGTTCCATCTGCCGCCTGGACTCCTCTAAATAGCATAAAGGTTGCGCTAACAGGAGCAGAAACTCTTATTCTTGCATCAGATCCGGATCGTGAAGGAGAGGCTATTGCTTGGCATATTATTGAGATGCTGCAACAACAGGATGCTTTACGTGAAGATATTACCGTAGCCAGGGTTGTTTTTCATGAAATCACTGAGGCCTCTATCAAAAGTGCGCTGCAAGCTCCAAGAGAAATTGATGAGAACTTGGTCCATGCTTACCTTGCACGGCGTGCACTTGATTATTTGATTGGATTTAACATTTCACCACTGTTATGGAGGAAACTACCAGGTTGCCAATCTGCGGGGCGAGTCCAATCTGCTGCACTGGCTCTTCTGTGCGATAGAGAAATGGAAATTGATGAATTTAAACCACAGGAATATTGGACTGCTGCGGTTGAATTGAAGAAAAAAGAAAATGTTTCATCAGTTAATGCTCCATCCTTCCCAGCACACTTGGCCCATTATGATTCCAAAAAGCTAAATCAATTTTCTTTCAGTTCTCATACCGAAGCAAAAGAAATTGAACAGCAGATTAATTCAGAAAATTTTCAAGTTGTTAATTCTAAGAAAAACAAAATGCGGAAGAATCCTCCCACGCCGTATATAACATCCACACTTCAGCAGGATGCTGCCAACAAATTGCATTTTAGTGCAGCATATACAATGAAGCTTGCACAGAAACTTTACGAGGGGGTTCAGTTATCTGATGGTAAAGCAATGGGTTTGATCACATACACTCGAACAGATGGAGTACATATTTCTGATGAAGCCTGTAAGGATGTGCGCTCCTTGGTCATTGAAAGGTATGGACAGAATTTTGCATCAGAAAGTGCACGCAAATTTTTTAAGAAAGTGAAGAATTCCCAAGAGGCGCATGAAGCAATCAGACCCACTGATGTTCGAAGATTGCCATCATTGCTCCGTGGGGTACTTGATGAAGATTCCCTGAAGTTGTACACTCTTATTTGGTGTCAGACAGTGGCATGTCAGATGGAGCCTGCTACAATTGAGCAGATACATCTTGATATTGGAAATGCTCACAACTCCATTGTTCTCCGCACTTCGTCCTCAAGGGTTGAGTTCCCTGGATATCAGGCAGTCTTCCGGGACATGGAAGCTCAAGCGGTCAAATATAAAGAACATGAAGAAAACAGTCATGAAGAAGCTTTTGTACTTCTTAATTCATTAAAGTATGGGGATCCATTAGATATTGGTAAACTGGAACTCAAGGAGCACCATACTCAACCTCCTCCCCGCTACTCTGAGGCAGCACTGATTAAGAAATTGGAGGAGCTTGGGATTGGAAGACCTTCAACATATGTGAGCACTCTGAAGGTTTTACAGGGTAGAAACTATGTGACAGTGAAAAGCCGTGTATTACATCCAGAATTTCGTGGTCGTATGGTGTCAGCATTTCTCAGCGACCATTTTTCCGGGGTCACAGATTATAGTTTTACTGCTGACATGGAGACTGAGCTTGATAATGTCTCTGCTGGCTTGACTGAATGGAAAGGCCTCCTTAAAGATTATTGGACCCGATTCAGCACTTATTGTGAACGTACCAGTACTGTTCATATTCATCAGGTAGAGAAGATGATGGAGAAGAAATTTGGAGACTTCTTGTTTGCTTCTCTTCCCGACCAAAGCAGGACATGCCCATATTGTGCGGAAGGCAGTTTGATTTTCAAAGTGAGTCGGTTCGGTGCTGGCTATTTTATAGGTTGTGATCAACACCCACAGTGCAAATACATTGCTAAGACATTCTACGGTGATGAAGAAGAAGAAGAAGATGATGGTCAAAAAAAGAACAGCAGCATGGAGGAACCAAAACTGCTAGGTCTAAGTCCACTTTCCGATGAAAAGGTTCTTTTGAAGAAAGGTCCTTACGGATGCTATGTACAACTTGGTGAAGACAGGAAGACTTATTTACCTAGAAGAGCCAATGCATCCCATATAAAGGATGTGAACTCCATTACCCTTGAATATGCGCTGGAGTTGCTGCGATATCCTGTGACATTGGGAAAACATCCAGCTGATGGACAACCAGTCATTTTAAAGGTTTCAAAGGTTGGGTTTGCGGTTAGACATCGGAGCACAATTGCTTCTGTTCCTAAGCATAAGAAACCCACTGAAATCACCCTCAAGGGTGCATTGAAGCTTTTGTCTGGTAATAATGTAAGAAGAATTGGTCGACCTACGAGAGAACAAATTGAAGAAGCCCAAGCAGCCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1174

Amino Acids

130.58

Weight (kDa)

9.33

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 365 - 479 8.2e-16 Toprim domain
Topoisom_bac PF01131 494 - 936 2.2e-109 DNA topoisomerase
Zn_ribbon_Top1 PF01396 979 - 1014 1.8e-07 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1045 - 1106 1e-08 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 845
AccB7I CCANNNNNTGG 1 cut(s) 3323
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 2 cut(s) 174, 3478
AccIII TCCGGA 3 cut(s) 209, 1205, 1330
AclWI GGATC 6 cut(s) 679, 1216, 1322, 1341, 2480, 2493
AcoI YGGCCR 2 cut(s) 96, 231
AcuI CTGAAG 4 cut(s) 1085, 1889, 2255, 2639
AdeI CACNNNGTG 1 cut(s) 1517
AfiI CCNNNNNNNGG 8 cut(s) 94, 1085, 1175, 1416, 1588, 2398, 3244, 3323
AhdI GACNNNNNGTC 1 cut(s) 3332
AjnI CCWGG 5 cut(s) 708, 1262, 1415, 1573, 2371
AjuI GAANNNNNNNTTGG 4 cut(s) 422, 454, 624, 656
Alw21I GWGCWC 5 cut(s) 1522, 2122, 2523, 2615, 3383
Alw26I GTCTC 5 cut(s) 86, 718, 2750, 2779, 2895
Alw44I GTGCAC 2 cut(s) 1518, 2118
AlwI GGATC 6 cut(s) 679, 1216, 1322, 1341, 2480, 2493
AlwNI CAGNNNCTG 1 cut(s) 794
Aor13HI TCCGGA 3 cut(s) 209, 1205, 1330
AoxI GGCC 7 cut(s) 96, 231, 251, 1217, 1443, 1743, 2800
ApaLI GTGCAC 2 cut(s) 1518, 2118
ArsI GACNNNNNNTTYG 2 cut(s) 2894, 2926
AseI ATTAAT 2 cut(s) 983, 1821
Asp700I GAANNNNTTC 5 cut(s) 184, 1164, 1833, 2596, 3150
Asp718I GGTACC 1 cut(s) 845
AspLEI GCGC 6 cut(s) 299, 1294, 1462, 2073, 2161, 3277
AspS9I GGNCC 6 cut(s) 251, 1203, 1495, 1744, 2820, 3167
AsuC2I CCSGG 2 cut(s) 2393, 2722
AsuHPI GGTGA 5 cut(s) 1160, 1542, 3068, 3206, 3415
AsuII TTCGAA 2 cut(s) 242, 2188
AvaII GGWCC 4 cut(s) 1203, 1495, 2820, 3167
BaeGI GKGCMC 2 cut(s) 1522, 2122
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BalI TGGCCA 1 cut(s) 98
BamHI GGATCC 1 cut(s) 2485
BanI GGYRCC 1 cut(s) 845
BarI GAAGNNNNNNTAC 4 cut(s) 1058, 1090, 2442, 2474
BbsI GAAGAC 4 cut(s) 2380, 2599, 3204, 3212
Bbv12I GWGCWC 5 cut(s) 1522, 2122, 2523, 2615, 3383
BceAI ACGGC 6 cut(s) 38, 246, 1232, 1529, 1870, 2643
BciT130I CCWGG 5 cut(s) 710, 1264, 1417, 1575, 2373
BclI TGATCA 3 cut(s) 225, 2013, 3013
BcnI CCSGG 2 cut(s) 2393, 2722
BcoDI GTCTC 5 cut(s) 86, 718, 2750, 2779, 2895
BfaI CTAG 5 cut(s) 594, 1170, 1200, 3122, 3219
BfmI CTRYAG 2 cut(s) 104, 756
BmcAI AGTACT 1 cut(s) 2854
Bme1390I CCNGG 7 cut(s) 710, 1264, 1417, 1575, 2373, 2393, 2722
Bme18I GGWCC 4 cut(s) 1203, 1495, 2820, 3167
BmeRI GACNNNNNGTC 1 cut(s) 3332
BmgT120I GGNCC 6 cut(s) 251, 1203, 1495, 1744, 2820, 3167
BmiI GGNNCC 5 cut(s) 847, 2283, 2487, 2822, 3111
BmrFI CCNGG 7 cut(s) 710, 1264, 1417, 1575, 2373, 2393, 2722
BmrI ACTGGG 1 cut(s) 1095
BmsI GCATC 7 cut(s) 1331, 1362, 1378, 1903, 2117, 3167, 3242
BmuI ACTGGG 1 cut(s) 1095
BpiI GAAGAC 4 cut(s) 2380, 2599, 3204, 3212
BpmI CTGGAG 3 cut(s) 438, 768, 3299
Bpu10I CCTNAGC 1 cut(s) 3399
Bpu14I TTCGAA 2 cut(s) 242, 2188
BpuEI CTTGAG 5 cut(s) 429, 2341, 2391, 2498, 3413
BpuMI CCSGG 2 cut(s) 2393, 2722
BsaAI YACGTR 1 cut(s) 1397
BsaBI GATNNNNATC 2 cut(s) 1332, 2197
BsaI GGTCTC 1 cut(s) 718
BsaJI CCNNGG 8 cut(s) 93, 708, 906, 1416, 2076, 2209, 2371, 2721
BsaWI WCCGGW 3 cut(s) 209, 1205, 1330
BsaXI ACNNNNNCTCC 4 cut(s) 462, 492, 2518, 2548
Bsc4I CCNNNNNNNGG 8 cut(s) 94, 1085, 1175, 1416, 1588, 2398, 3244, 3323
Bse1I ACTGG 5 cut(s) 1090, 1617, 2511, 2850, 3332
Bse3DI GCAATG 3 cut(s) 2010, 2201, 3036
Bse8I GATNNNNATC 2 cut(s) 1332, 2197
BseAI TCCGGA 3 cut(s) 209, 1205, 1330
BseBI CCWGG 5 cut(s) 710, 1264, 1417, 1575, 2373
BseDI CCNNGG 8 cut(s) 93, 708, 906, 1416, 2076, 2209, 2371, 2721
BseJI GATNNNNATC 2 cut(s) 1332, 2197
BseLI CCNNNNNNNGG 8 cut(s) 94, 1085, 1175, 1416, 1588, 2398, 3244, 3323
BseMI GCAATG 3 cut(s) 2010, 2201, 3036
BseMII CTCAG 5 cut(s) 203, 1431, 2541, 2719, 2751
BseNI ACTGG 5 cut(s) 1090, 1617, 2511, 2850, 3332
BseRI GAGGAG 3 cut(s) 1260, 2526, 2591
BseSI GKGCMC 2 cut(s) 1522, 2122
BseYI CCCAGC 2 cut(s) 939, 1732
BsgI GTGCAG 2 cut(s) 1593, 1959
BshFI GGCC 7 cut(s) 98, 233, 253, 1219, 1445, 1745, 2802
BshNI GGYRCC 1 cut(s) 845
BsiHKAI GWGCWC 5 cut(s) 1522, 2122, 2523, 2615, 3383
BsiSI CCGG 5 cut(s) 210, 1206, 1331, 2392, 2721
BslFI GGGAC 2 cut(s) 1202, 2408
BslI CCNNNNNNNGG 8 cut(s) 94, 1085, 1175, 1416, 1588, 2398, 3244, 3323
BsmAI GTCTC 5 cut(s) 86, 718, 2750, 2779, 2895
BsmFI GGGAC 2 cut(s) 1202, 2408
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 7 cut(s) 98, 233, 253, 1219, 1445, 1745, 2802
Bso31I GGTCTC 1 cut(s) 718
Bsp119I TTCGAA 2 cut(s) 242, 2188
Bsp1286I GDGCHC 5 cut(s) 1522, 2122, 2523, 2615, 3383
Bsp13I TCCGGA 3 cut(s) 209, 1205, 1330
Bsp1407I TGTACA 2 cut(s) 2241, 3184
Bsp143I GATC 8 cut(s) 225, 671, 1208, 1327, 1333, 2013, 2485, 3013
Bsp19I CCATGG 1 cut(s) 93
BspANI GGCC 7 cut(s) 98, 233, 253, 1219, 1445, 1745, 2802
BspCNI CTCAG 5 cut(s) 202, 1432, 2542, 2718, 2752
BspEI TCCGGA 3 cut(s) 209, 1205, 1330
BspHI TCATGA 2 cut(s) 1429, 2443
BspLI GGNNCC 5 cut(s) 847, 2283, 2487, 2822, 3111
BspMAI CTGCAG 1 cut(s) 108
BspPI GGATC 6 cut(s) 679, 1216, 1322, 1341, 2480, 2493
BspQI GCTCTTC 3 cut(s) 737, 1623, 3217
BspT104I TTCGAA 2 cut(s) 242, 2188
BspT107I GGYRCC 1 cut(s) 845
BspTNI GGTCTC 1 cut(s) 718
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 3 cut(s) 2010, 2201, 3036
BsrGI TGTACA 2 cut(s) 2241, 3184
BsrI ACTGG 5 cut(s) 1090, 1617, 2511, 2850, 3332
BssECI CCNNGG 8 cut(s) 93, 708, 906, 1416, 2076, 2209, 2371, 2721
BssMI GATC 8 cut(s) 225, 671, 1208, 1327, 1333, 2013, 2485, 3013
BssNAI GTATAC 1 cut(s) 175
BssT1I CCWWGG 2 cut(s) 93, 2076
Bst1107I GTATAC 1 cut(s) 175
Bst2UI CCWGG 5 cut(s) 710, 1264, 1417, 1575, 2373
Bst4CI ACNGT 9 cut(s) 1096, 1411, 1557, 2266, 2441, 2650, 2857, 3027, 3056
Bst6I CTCTTC 4 cut(s) 737, 1623, 2925, 3217
BstAPI GCANNNNNTGC 3 cut(s) 794, 1517, 2940
BstAUI TGTACA 2 cut(s) 2241, 3184
BstBAI YACGTR 1 cut(s) 1397
BstBI TTCGAA 2 cut(s) 242, 2188
BstC8I GCNNGC 9 cut(s) 139, 351, 1467, 1518, 1959, 2122, 2286, 2782, 2995
BstDSI CCRYGG 3 cut(s) 93, 906, 2209
BstHHI GCGC 6 cut(s) 299, 1294, 1462, 2073, 2161, 3277
BstKTI GATC 8 cut(s) 228, 674, 1211, 1330, 1336, 2016, 2488, 3016
BstMAI GTCTC 5 cut(s) 86, 718, 2750, 2779, 2895
BstMBI GATC 8 cut(s) 225, 671, 1208, 1327, 1333, 2013, 2485, 3013
BstNI CCWGG 5 cut(s) 710, 1264, 1417, 1575, 2373
BstNSI RCATGY 2 cut(s) 2274, 2943
BstSCI CCNGG 7 cut(s) 708, 1262, 1415, 1573, 2371, 2391, 2720
BstSFI CTRYAG 2 cut(s) 104, 756
BstSLI GKGCMC 2 cut(s) 1522, 2122
BstV2I GAAGAC 4 cut(s) 2380, 2599, 3204, 3212
BstX2I RGATCY 4 cut(s) 671, 1208, 1327, 2485
BstXI CCANNNNNNTGG 1 cut(s) 1560
BstYI RGATCY 4 cut(s) 671, 1208, 1327, 2485
BstZ17I GTATAC 1 cut(s) 175
BsuRI GGCC 7 cut(s) 98, 233, 253, 1219, 1445, 1745, 2802
BtgI CCRYGG 3 cut(s) 93, 906, 2209
BtgZI GCGATG 2 cut(s) 20, 297
BtsI GCAGTG 2 cut(s) 113, 891
Cac8I GCNNGC 9 cut(s) 139, 351, 1467, 1518, 1959, 2122, 2286, 2782, 2995
CaiI CAGNNNCTG 1 cut(s) 794
CciI TCATGA 2 cut(s) 1429, 2443
CfoI GCGC 6 cut(s) 299, 1294, 1462, 2073, 2161, 3277
Cfr13I GGNCC 6 cut(s) 251, 1203, 1495, 1744, 2820, 3167
CsiI ACCWGGT 1 cut(s) 1573
DpnI GATC 8 cut(s) 227, 673, 1210, 1329, 1335, 2015, 2487, 3015
DpnII GATC 8 cut(s) 225, 671, 1208, 1327, 1333, 2013, 2485, 3013
DraI TTTAAA 3 cut(s) 321, 1654, 3342
DraIII CACNNNGTG 1 cut(s) 1517
DriI GACNNNNNGTC 1 cut(s) 3332
EaeI YGGCCR 2 cut(s) 96, 231
Eam1104I CTCTTC 4 cut(s) 737, 1623, 2925, 3217
Eam1105I GACNNNNNGTC 1 cut(s) 3332
EarI CTCTTC 4 cut(s) 737, 1623, 2925, 3217
EciI GGCGGA 2 cut(s) 61, 74
Eco130I CCWWGG 2 cut(s) 93, 2076
Eco147I AGGCCT 2 cut(s) 1445, 2802
Eco31I GGTCTC 1 cut(s) 718
Eco32I GATATC 2 cut(s) 2378, 3293
Eco47I GGWCC 4 cut(s) 1203, 1495, 2820, 3167
Eco57I CTGAAG 4 cut(s) 1085, 1889, 2255, 2639
EcoO109I RGGNCCY 1 cut(s) 3167
EcoRI GAATTC 1 cut(s) 2146
EcoRII CCWGG 5 cut(s) 708, 1262, 1415, 1573, 2371
EcoRV GATATC 2 cut(s) 2378, 3293
EcoT14I CCWWGG 2 cut(s) 93, 2076
EcoT22I ATGCAT 1 cut(s) 3235
ErhI CCWWGG 2 cut(s) 93, 2076
FalI AAGNNNNNCTT 2 cut(s) 2790, 2822
FaqI GGGAC 2 cut(s) 1202, 2408
FauI CCCGC 2 cut(s) 1582, 2549
FauNDI CATATG 1 cut(s) 2605
FbaI TGATCA 3 cut(s) 225, 2013, 3013
FblI GTMKAC 2 cut(s) 174, 3478
FspBI CTAG 5 cut(s) 594, 1170, 1200, 3122, 3219
GlaI GCGC 6 cut(s) 298, 1293, 1461, 2072, 2160, 3276
GsaI CCCAGC 2 cut(s) 943, 1736
GsuI CTGGAG 3 cut(s) 438, 768, 3299
HaeIII GGCC 7 cut(s) 98, 233, 253, 1219, 1445, 1745, 2802
HapII CCGG 5 cut(s) 210, 1206, 1331, 2392, 2721
HhaI GCGC 6 cut(s) 299, 1294, 1462, 2073, 2161, 3277
Hin6I GCGC 6 cut(s) 297, 1292, 1460, 2071, 2159, 3275
HinP1I GCGC 6 cut(s) 297, 1292, 1460, 2071, 2159, 3275
HincII GTYRAC 1 cut(s) 3479
HindII GTYRAC 1 cut(s) 3479
HindIII AAGCTT 3 cut(s) 1955, 2451, 3443
HinfI GANTC 9 cut(s) 590, 1109, 1267, 1596, 1754, 1873, 2228, 2826, 2980
HpaII CCGG 5 cut(s) 210, 1206, 1331, 2392, 2721
HphI GGTGA 5 cut(s) 1160, 1542, 3068, 3206, 3415
Hpy188III TCNNGA 9 cut(s) 210, 1206, 1331, 1337, 1430, 2309, 2444, 2672, 2924
HpyCH4III ACNGT 9 cut(s) 1096, 1411, 1557, 2266, 2441, 2650, 2857, 3027, 3056
HpyCH4IV ACGT 2 cut(s) 1396, 2845
HpySE526I ACGT 2 cut(s) 1396, 2845
HspAI GCGC 6 cut(s) 297, 1292, 1460, 2071, 2159, 3275
Kpn2I TCCGGA 3 cut(s) 209, 1205, 1330
KpnI GGTACC 1 cut(s) 849
Ksp22I TGATCA 3 cut(s) 225, 2013, 3013
Kzo9I GATC 8 cut(s) 225, 671, 1208, 1327, 1333, 2013, 2485, 3013
LguI GCTCTTC 3 cut(s) 737, 1623, 3217
LweI GCATC 7 cut(s) 1331, 1362, 1378, 1903, 2117, 3167, 3242
MabI ACCWGGT 1 cut(s) 1573
MaeI CTAG 5 cut(s) 594, 1170, 1200, 3122, 3219
MaeII ACGT 2 cut(s) 1396, 2845
MaeIII GTNAC 5 cut(s) 801, 817, 2644, 2725, 3298
MalI GATC 8 cut(s) 227, 673, 1210, 1329, 1335, 2015, 2487, 3015
MbiI CCGCTC 1 cut(s) 90
MboI GATC 8 cut(s) 225, 671, 1208, 1327, 1333, 2013, 2485, 3013
MfeI CAATTG 2 cut(s) 2292, 3384
MflI RGATCY 4 cut(s) 671, 1208, 1327, 2485
MhlI GDGCHC 5 cut(s) 1522, 2122, 2523, 2615, 3383
MlsI TGGCCA 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 4 cut(s) 1118, 1261, 1605, 2989
MmeI TCCRAC 1 cut(s) 622
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 1 cut(s) 3235
MroI TCCGGA 3 cut(s) 209, 1205, 1330
MroXI GAANNNNTTC 5 cut(s) 184, 1164, 1833, 2596, 3150
MscI TGGCCA 1 cut(s) 98
MslI CAYNNNNRTG 2 cut(s) 1558, 1935
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 3 cut(s) 869, 1072, 3320
MspI CCGG 5 cut(s) 210, 1206, 1331, 2392, 2721
MspR9I CCNGG 7 cut(s) 710, 1264, 1417, 1575, 2373, 2393, 2722
MunI CAATTG 2 cut(s) 2292, 3384
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 5 cut(s) 710, 1264, 1417, 1575, 2373
NciI CCSGG 2 cut(s) 2393, 2722
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 2605
NdeII GATC 8 cut(s) 225, 671, 1208, 1327, 1333, 2013, 2485, 3013
NlaIV GGNNCC 5 cut(s) 847, 2283, 2487, 2822, 3111
NmuCI GTSAC 4 cut(s) 801, 2644, 2725, 3298
NsiI ATGCAT 1 cut(s) 3235
NspI RCATGY 2 cut(s) 2274, 2943
NspV TTCGAA 2 cut(s) 242, 2188
PagI TCATGA 2 cut(s) 1429, 2443
PceI AGGCCT 2 cut(s) 1445, 2802
PciSI GCTCTTC 3 cut(s) 737, 1623, 3217
PctI GAATGC 1 cut(s) 299
PdmI GAANNNNTTC 5 cut(s) 184, 1164, 1833, 2596, 3150
PfeI GAWTC 5 cut(s) 590, 1754, 1873, 2228, 2826
PflMI CCANNNNNTGG 1 cut(s) 3323
PfoI TCCNGGA 1 cut(s) 2391
PleI GAGTC 4 cut(s) 1117, 1261, 1604, 2988
PpsI GAGTC 4 cut(s) 1117, 1261, 1604, 2988
Ppu21I YACGTR 1 cut(s) 1397
PpuMI RGGWCCY 1 cut(s) 3167
PshBI ATTAAT 2 cut(s) 983, 1821
Psp5II RGGWCCY 1 cut(s) 3167
Psp6I CCWGG 5 cut(s) 708, 1262, 1415, 1573, 2371
PspFI CCCAGC 2 cut(s) 939, 1732
PspGI CCWGG 5 cut(s) 708, 1262, 1415, 1573, 2371
PspN4I GGNNCC 5 cut(s) 847, 2283, 2487, 2822, 3111
PspPI GGNCC 6 cut(s) 251, 1203, 1495, 1744, 2820, 3167
PspPPI RGGWCCY 1 cut(s) 3167
PstI CTGCAG 1 cut(s) 108
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 4 cut(s) 671, 1208, 1327, 2485
PvuII CAGCTG 2 cut(s) 1072, 3320
RseI CAYNNNNRTG 2 cut(s) 1558, 1935
SalI GTCGAC 1 cut(s) 3477
SapI GCTCTTC 3 cut(s) 737, 1623, 3217
Sau3AI GATC 8 cut(s) 225, 671, 1208, 1327, 1333, 2013, 2485, 3013
Sau96I GGNCC 6 cut(s) 251, 1203, 1495, 1744, 2820, 3167
ScaI AGTACT 1 cut(s) 2854
SchI GAGTC 4 cut(s) 1118, 1261, 1605, 2989
ScrFI CCNGG 7 cut(s) 710, 1264, 1417, 1575, 2373, 2393, 2722
SduI GDGCHC 5 cut(s) 1522, 2122, 2523, 2615, 3383
SexAI ACCWGGT 1 cut(s) 1573
SfaNI GCATC 7 cut(s) 1331, 1362, 1378, 1903, 2117, 3167, 3242
SfcI CTRYAG 2 cut(s) 104, 756
SfuI TTCGAA 2 cut(s) 242, 2188
SinI GGWCC 4 cut(s) 1203, 1495, 2820, 3167
SmiMI CAYNNNNRTG 2 cut(s) 1558, 1935
SmlI CTYRAG 5 cut(s) 444, 2356, 2406, 2513, 3428
SmoI CTYRAG 5 cut(s) 444, 2356, 2406, 2513, 3428
SseBI AGGCCT 2 cut(s) 1445, 2802
SspI AATATT 1 cut(s) 1667
SspMI CTAG 5 cut(s) 594, 1170, 1200, 3122, 3219
StuI AGGCCT 2 cut(s) 1445, 2802
StyD4I CCNGG 7 cut(s) 708, 1262, 1415, 1573, 2371, 2391, 2720
StyI CCWWGG 2 cut(s) 93, 2076
TaaI ACNGT 9 cut(s) 1096, 1411, 1557, 2266, 2441, 2650, 2857, 3027, 3056
TaiI ACGT 2 cut(s) 1399, 2848
TaqI TCGA 5 cut(s) 242, 740, 2026, 2188, 3478
TatI WGTACW 5 cut(s) 2038, 2241, 2458, 2852, 3184
TauI GCSGC 2 cut(s) 90, 1262
TfiI GAWTC 5 cut(s) 590, 1754, 1873, 2228, 2826
TseFI GTSAC 4 cut(s) 801, 2644, 2725, 3298
Tsp45I GTSAC 4 cut(s) 801, 2644, 2725, 3298
TspGWI ACGGA 2 cut(s) 2198, 3189
Van91I CCANNNNNTGG 1 cut(s) 3323
VneI GTGCAC 2 cut(s) 1518, 2118
VpaK11BI GGWCC 4 cut(s) 1203, 1495, 2820, 3167
VspI ATTAAT 2 cut(s) 983, 1821
XceI RCATGY 2 cut(s) 2274, 2943
XcmI CCANNNNNNNNNTGG 2 cut(s) 1260, 1665
XmiI GTMKAC 2 cut(s) 174, 3478
XmnI GAANNNNTTC 5 cut(s) 184, 1164, 1833, 2596, 3150
XspI CTAG 5 cut(s) 594, 1170, 1200, 3122, 3219
ZrmI AGTACT 1 cut(s) 2854
Zsp2I ATGCAT 1 cut(s) 3235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.