pycom15g11180

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014812
Physical Location & Seq
Reverse (-)
7552976 .. 7554362
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
ATGCAGGACCGACTCGTTAGCCTTTTGATATTTAGCATTGTGCTGTCTGGGTTGATTGATATTGCGTACAGTGAAAAAACATTTGATGTTCTTAATTATAGAGCAGTTGGGGATGGAGAAGCTGACGATTCTGAAGCTTTTGTAAATGCATGGAAAGCCTTATGTGGAGCAGATGAAGTTGGTGACGTACCTACACTAGTTGTACCCAAGGGAAAAACATTCTTACTGCAGCCTATCGAATTTGAAGGCCCTTGCAGGCCTAAGAGGGTCCATATTCAGGCTCAGGTTCATCCATGGCTATTGTCCGTTTATTGTTCCTGGGGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

110

Amino Acids

12.1

Weight (kDa)

4.8

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 3 cut(s) 68, 189, 204
AfiI CCNNNNNNNGG 1 cut(s) 277
AgsI TTSAA 1 cut(s) 245
AhlI ACTAGT 1 cut(s) 196
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 2 cut(s) 122, 137
AluI AGCT 2 cut(s) 122, 137
AoxI GGCC 2 cut(s) 247, 257
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 239
AspS9I GGNCC 3 cut(s) 7, 248, 268
AsuHPI GGTGA 1 cut(s) 194
AvaII GGWCC 2 cut(s) 7, 268
BbvI GCAGC 1 cut(s) 241
BccI CCATC 1 cut(s) 107
BciT130I CCWGG 1 cut(s) 319
BcuI ACTAGT 1 cut(s) 196
BfaI CTAG 1 cut(s) 197
BfmI CTRYAG 1 cut(s) 227
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
Bme1390I CCNGG 1 cut(s) 319
Bme18I GGWCC 2 cut(s) 7, 268
BmgT120I GGNCC 3 cut(s) 7, 248, 268
BmiI GGNNCC 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 207, 293, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 3 cut(s) 207, 293, 318
BseGI GGATG 2 cut(s) 118, 289
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 296
BseXI GCAGC 1 cut(s) 241
BshFI GGCC 2 cut(s) 249, 259
BslI CCNNNNNNNGG 1 cut(s) 277
BsnI GGCC 2 cut(s) 249, 259
Bsp19I CCATGG 1 cut(s) 293
BspANI GGCC 2 cut(s) 249, 259
BspCNI CTCAG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 269
BspMAI CTGCAG 1 cut(s) 231
BssECI CCNNGG 3 cut(s) 207, 293, 318
BssT1I CCWWGG 2 cut(s) 207, 293
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 2 cut(s) 261, 282
BstDSI CCRYGG 1 cut(s) 293
BstF5I GGATG 2 cut(s) 118, 289
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 317
BstSFI CTRYAG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 241
BsuRI GGCC 2 cut(s) 249, 259
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 2 cut(s) 118, 289
BtsIMutI CAGTG 1 cut(s) 76
Cac8I GCNNGC 1 cut(s) 257
Cfr13I GGNCC 3 cut(s) 7, 248, 268
Csp6I GTAC 3 cut(s) 67, 188, 203
CviAII CATG 2 cut(s) 150, 294
CviJI RGCY 9 cut(s) 21, 122, 137, 158, 232, 249, 259, 281, 298
CviKI_1 RGCY 9 cut(s) 21, 122, 137, 158, 232, 249, 259, 281, 298
CviQI GTAC 3 cut(s) 67, 188, 203
DdeI CTNAG 2 cut(s) 261, 282
Eco130I CCWWGG 2 cut(s) 207, 293
Eco147I AGGCCT 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 7, 268
Eco57I CTGAAG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 2 cut(s) 207, 293
EcoT22I ATGCAT 1 cut(s) 151
ErhI CCWWGG 2 cut(s) 207, 293
FaeI CATG 2 cut(s) 153, 297
FaiI YATR 5 cut(s) 99, 151, 163, 273, 295
FatI CATG 2 cut(s) 149, 293
Fnu4HI GCNGC 1 cut(s) 230
FokI GGATG 2 cut(s) 125, 276
Fsp4HI GCNGC 1 cut(s) 230
FspBI CTAG 1 cut(s) 197
GluI GCNGC 1 cut(s) 230
HaeIII GGCC 2 cut(s) 249, 259
Hin1II CATG 2 cut(s) 153, 297
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 2 cut(s) 12, 128
HphI GGTGA 1 cut(s) 194
Hpy188I TCNGA 1 cut(s) 133
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 4 cut(s) 4, 149, 229, 255
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 261, 282
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 2 cut(s) 153, 297
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 5 cut(s) 33, 241, 263, 269, 304
Lsp1109I GCAGC 1 cut(s) 241
MaeI CTAG 1 cut(s) 197
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 182
MluCI AATT 2 cut(s) 94, 239
MlyI GAGTC 1 cut(s) 6
MnlI CCTC 1 cut(s) 258
Mph1103I ATGCAT 1 cut(s) 151
MseI TTAA 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 319
MvaI CCWGG 1 cut(s) 319
MwoI GCNNNNNNNGC 1 cut(s) 155
NcoI CCATGG 1 cut(s) 293
NlaIII CATG 2 cut(s) 153, 297
NlaIV GGNNCC 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 182
NsiI ATGCAT 1 cut(s) 151
PceI AGGCCT 1 cut(s) 259
PfeI GAWTC 1 cut(s) 128
PkrI GCNGC 1 cut(s) 231
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 269
PspPI GGNCC 3 cut(s) 7, 248, 268
PstI CTGCAG 1 cut(s) 231
RsaI GTAC 3 cut(s) 68, 189, 204
RsaNI GTAC 3 cut(s) 67, 188, 203
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 1 cut(s) 230
Sau96I GGNCC 3 cut(s) 7, 248, 268
SchI GAGTC 1 cut(s) 6
ScrFI CCNGG 1 cut(s) 319
SetI ASST 5 cut(s) 124, 139, 189, 193, 288
SfcI CTRYAG 1 cut(s) 227
SinI GGWCC 2 cut(s) 7, 268
SpeI ACTAGT 1 cut(s) 196
Sse9I AATT 2 cut(s) 94, 239
SseBI AGGCCT 1 cut(s) 259
SspMI CTAG 1 cut(s) 197
StuI AGGCCT 1 cut(s) 259
StyD4I CCNGG 1 cut(s) 317
StyI CCWWGG 2 cut(s) 207, 293
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 237
TaqII GACCGA 1 cut(s) 24
TasI AATT 2 cut(s) 94, 239
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 189, 278
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 1 cut(s) 76
VpaK11BI GGWCC 2 cut(s) 7, 268
XapI RAATTY 1 cut(s) 239
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.