pycom15g11740

PHD-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004222
Physical Location & Seq
Forward (+)
7945602 .. 7956475
10874 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6225 bp
ATGGAGAGCTCGTGGAAGCCTAAATATAGTTCGACATTCCCGTCAATGGTTTCCTCGTCCTCTCAGGAGCTTCGGAATCAGATGGAGAGCAATGCAGGGTATTATTCTTATCCACATGGCTCACAAGATTTAAGACCCACAATGCTTGGTAGATTACAAGATCCTGTACTTTCAAGCATTCCGTATTCAGATTCCCACAGATCAGAACATGCTACTTTGGGAAATTCATTTCTAGCTCTCTTATCTGGGTCACAATCGGTATTCCAGTGTGATTTTCAAGGATTATCAAATCCAAAGCCTATGGGTACCTCTTGTAAGCGTCTGCCAGATAGTAATAATTTCATTGTGAATGGTACCGGAAGTGCCATTCCAGTCACATCCAGTGGGCTACTCCCAGAAAATATGAGTGGGCAACACCTGCAAGGCGGAGCAGGCTCTTTTAAGGTTTCATCAAAGTCAGTCCCAAGCTCCAGTTTTGGAAGCAAATCTGTTTTGTATGATCTGCAGAATTCGGACCTGGCCAAGGTAGTCACATGTGACATGGTCTCTGGTAGTGAGAAGGTAAGGGGCTCTTTTTCCTTGAGCGGGGAATGGCATGGTGTTAGCGCTGCAGATACTAGGAAGGCAGCGAAGGCTTGTGGAGCAAATATTCAATCGTCAAGAACAAAGTCAATGGATGCAAGCACCTCTATATCTAACCAAGCATTGGGTTTTATGAATGGTTGCCCTCGTGTTTTTTGTTCAACCACTAGTGGTTATCTACTTCTCAGCAATACAGGACTTGTTGGAATTGTTTGTTCATGCCATTGTCTCCACATGTCTGTCCTGAAATTTTGCGAGCATTCAGGGTTATATGATGTCAACCCGGGGGACGTCGTTCGTATGGACAGTGGAGAGACCATTGCCCAGTGGTGCAAGCTCTACTTCCTGAATTCTGGGATTAGGGTTCCGGGAGATCGGAGTGAATGGGACTGGCCTGAAGGATTATCAGCAACTGCCTCTTTGGCGAAATCTAGTGTGACTATGCCCAACATATCCAATAACTTGTCTCATATGGTTCTCTCATCTGGAGGTTCAGCTAGTTCTCAGCGGTCTTTAGATGGTGCTGCGCTCTTAAAGAGCCTCCTTACAAACCAGAATTCTGCTATTGGTTCTGTACAAAATAAACAACAAAGGAACATTCAGGATAGCAACAACATTTCTCTCAAGGGTTTGACTGGCACTTCACAGAACAATTTACATGGTATGGCTGATAACCTGATATTGGAGGGCACTAAGTCAATCTCAAATTTGGTTGGTTGTGGACTGCAAGATGGCTGTCAGTCCGCATCTGCTTACGTTGAGTCCATGAAGAATGGAAATTCATCCATTACCTACCCCACCATGAAGAATGGAAATTCATCCATTACACACCATTCTTTACAGAACCGAAGAATCATTGGTACAGAATCAAACGTTTGCAGAACTGTAAATGGGAAGGATGGTGCCTTAAGGGATGCTGCAATTTCAAATATTGAGCTGAGGCTTGGTCAACCATACCAGTTGGGTCAAACTCAAACTTCAGGAAATTCAAATCTATCAGCTGTACGTCCACAGCTATTGGACACACATGCAAATCCACTGAAGTCTCTCTTTCCTGAACAGATGATTCCTAACACAGCCAACTGCAGTGAAGGGGTGAAATTTGGCCAATCTCTTCAGTTTTCTGCTGTTCCATATAATCCCAGCACAAAAAGAGATGGAAAGCAGTTAAACCGTGGAAATAATGCATTTGTAATCAGTAATGCGATAGATGCTGCAAGAGTAGAGAAAACCAATTGCAACCTGGTCCAGGATTCTGTGATTTCATTTCTTACCAATCTCAATGCTCCACCTGATGAAAATTCACGACCAAAAGCCAGTAATTATATATATAATGGAGGTGAGCATGCTATGCGAAGTACACAACATTATGAAACTCATTCTGCCAAGTCTGAGCTCACTAGTGGCCCCCGGAATGGAGGTAATAATTTACAAAGGCAATTGAACATGTCTCAGTTGGGTTCCTATAGACTAATTGACAAGGCTAAAGGGGCAAGTTTTGTCGCTAATGATTCTCACCTTTCAAAGGATTCAGGGTGTAGAATTCACAAGGAAATTGAAATCTCAAGCTCTTTCAATGGATTGAGTGGAAGCAGTGATCCTCATTTCTTAACAGTACATAAAGACAGCTGCTACTCACACCAGTTGTCTGGGGTAGCACCCAATGGACCTGATTCTAGAAGATATTCTAATTTTCCTGTGAAGATTTGTTCTTTTAGTAATAGTGGGCAAGTTGGTCATGTTGATCTCCCACCCTTGACTTCATCGGTAGGATCTGGCATAATTTTTTCATCAGATGGAGTCTCAAAATGCATTCCTGTTAGTGTTTCAACATCTGTATCAGATCAGAGTCATCATCCACCTGCTTTATCTAGAGAACAGTTAACTGGAGTAACCACTCACTTGCCCGATGATAATTCACGACTGCTTGCAATAAGGCAAATACTGGAGTTATCGAAGCAACACCAGGCATGGCCTTCCCTTCCCATGAATCGAGGGGAAGGAAGATTTGGTTGCTCTTCTTATATGCAGAATTCTCTTGTTGACACTTCAGCATCCGAGAAGCAAGTCCAACAACTTAGTCATACCAGTAACCATGATGTCACCATTAAATCACACCAACCAGGTGCTAGCTGTAGAATTGGAACTGATGAAGGTTTTGCTTCCATGACTGGTGTTAACACCTGCTGTCATTTGTCAACATTGAAACAAGGAACTGCATTACATTCTAAAGAAGTTGATATGAATCATCAAATTTCCTTTGTTCCTCTGTTTAATGAGCAACACTCATTGAGAAGTGGGAAGAACACCAATGAGCCTAGAGGACATGAAAGATGCTACCACAAAGTTCCCTATGGTTGTTTTCATGGCAGCTGCAGTTGTGCGGCCAACATAAACTGTTTAGGAATGAATTTTGAAAGAGTTGGAGGTTTTCCTAATGCCTTTAATGAACAAATTGGAACAGTCCATGGTGAATCCCCCACGACATTTACTCATAAATGTGCTAACAACCATATTGTTCCAAAGGATAAAACTGTTTCCTTAGATCATAGGGGAAAGTTGAATGGGAAATTACCCAAGAACATCTGTAATGCTTCACAGTGGAAAGATGTTCCGAGTAAGGTCAAAGGAGTTTCTGATGTGATGCGTGTGGACCGTTTATCTAATTTGTTTGATGCGAGAAGACGAGATAGTGAGCAGCTTGCAGATACTAGTGTTAAACACTCCAATGGTACTTTGCAGATGGTGGAATCTTCGAAAGAGCATGAAATTTCTAATATTTCTTCTGGATGTTCTGTGCCGGCTGTTACCCAGGCATCAATTGAGGTTAACAAGATAGATTCTTCAACAGTTGATGCTGGGGATAATGGATGTGTAAGTAATCTTGTTTTTGACGAAGGGTCAGGCATTGGTAAATGCGGGTCATCAGATGATGCACTTGAAAGTGTAAAAAGTGCGGAGTTCTTTGCCTCAACTAGCTTGAGAAAACTTGGGTCTTTTAAGAATTTAAATCATCAATCATCTAGCCTTCTGGATGAGCTTAAGCTCCTAAACTCTTTAACATGGAGAAAAGGTCAACAGAAAATCCCAGCTGGGCTTGACAAATATCCCCAGAACTCTGAGAGAGACTCGGAAACTGGGAAGAAAAAGAGAGAAATGGGTTCCAAGACGTGTCCCACTTCAGGTCCTTATCCTGTACGAGAAGAAACCCCCAAATGTAATGACCCTGTTCAGGTTCCCTCTCATCCGTCAAAATGTGTGAAGATGCTCATTCCATCGGGGCAATCCAAAACTCATACCTTTGGGGCCTGTGCGACTCAGTCCAGTTCCAAACCCAGACTGCCTATATCGTCTTCAGAAAAGAAGCTTTCACGTAAAAGAGATTTGCGCAAATTCTATGATGACAATGACACAGAGGAAACTGATCTTTACCAGACAGAACCAAGTGGTGGTGCCAACTGTGATACTGCCGAAGTTTCTGGGGGAAAGAAATGTAAGAGGGATTTCAATTCTAACGGTTCGAGGCATGTCAAAATACAAGAATCAGGTGAAGAAGGTGCAAGAAAAAGAAAGCAAACATCTGTAGGTTGTTTGAAATCATGTTCTATTCAGCTGGTGAATACTTGTTATAGGAAGGCAAGACCGATAGTATGCGGAAAATATGGTGAACTAGCTAATGGAAGTTTGCCTGGAGACACGCCAAAACCAGCAAAAATTGTTCCCCTAAGCAAGGTTCTTGATTCTTCTAGAAGATGCAAGGTCCCGAAAGATTGTAATCCTAAATCACTTCAGAAAACAATCCCTGGTGGGGCTGTTGTTTGTGGTAAACTAAATGGTACTTCCATGGAAAAAACGAAGAAAGGGTGCTCCATTGGAGAAAAGAAATGTCGTAAAGAGTTGTCCTCATTGGAGAAATTGGGAGATAATCAACGTGAGAAAGATCATGGTAAGCTAGGCGTTATTGCCCATGCTCAATTGAAGCTGAAGTCTAAGGAAATTCGCAAGCGTAGCATTTATGAATTAACCGAGAAAGGGAAGGATCCCAGCTTTGAAAGTGCTTCTGCTTCAAAGATTTCAAATTGTCTGCCAGCAAAGAGAGAAGGACATCTCCAAAAGAGTGGTGAAAATAAGCTTGGACTCTGCAAACTTAGTGCTAAAATTTCCACTCTAGAGCAATGGTGCCATTCTGATTTGGATTCAGATGCATTCTGCTGTGTTTGTGGTAGCTCAAACAATGATGAAATCAATTATTTACTGGAGTGTAGTCAATGTTCAATCAAGGTGCATCAGGCTTGTTATGGTGTCTCAAAACAACCTAAAGGCCATTGGTATTGCAGGCCATGCCGGACCAGTTCAAAAAATATTGTTTGTGTTCTGTGTGGTTATGGTGGTGGTGCCATGACTCAAGCGCTGCGAAGTCACTCAGTTGTGAAAAGCCTTTTGAAAGCTTGGAACACTGAAACAGAAAGCATGCCCAAGAATAAGTTTTCATCAGTTAAAACTTTGCAAAAGGATTTGAGTGGGTGGCATTGCTTGACCTCAGCTGTCTGTTTTATGGATATGTCCTACCAAGTTGATGTCGTACATAATAGCATTACAGCGGGATTACTTGATTCGGCAACTAAGCAATGGGTTCATATGGTTTGTGGGCTTTGGACTCCTGGAACACGGTGCCCAAATGTTGACACCATGAGTGCTTTTGATGTCTCTGGTGCACATCCTAGAGCAGATGTGGTTTGTTGTATATGTATGCGAGCAGGCGGTTCATGTATACAGTGCAGGATTGCAAATTGTTCTGCTCAGTTTCACCCTTGGTGTGCTCATCAGAAGGGTCTGTTGCAAAGTGAAGCTGAGGGTGTTGACAATGAAAATATTGGTTTTTATGGAAAATGCGTGCTACATGCTACATACCCAATGTGCGAATCTGGCCATGTCCCTGAAACTGGTTGCATTGAAAAACAGGAATTAACTTGCGCTAGAACTGAGGGTTACAAGGGCCGTAAACGTGATGGGTTCAGGCATAATCATTATGGTCAGTCAAAGGGCAGTGGTGGATGCCTTGTTCCTCAGGAGCAGTTAAATGCTTGGGTTCATATTAATGGTCAGAAGTCATCCACTCAGGGGCTTCCAAAGCTGCCAGCCTCGGATATAGAGCATGATTGTCGGAAAGAATATGCACGCTACAAGCAAGCAAAGTTTTGGAAGCATCTAGTAGTGTACAAGTCTGGCATACATGCCCTTGGCCTTTACACGTCTCGGTTCATTTCCCGCAGTGAAATGGTTGTTGAGTATATTGGAGAGATAGTGGGGCTGCGTGTTGCCGACAAAAGGGAAAATGAATACCAATCTGGTAAAAAGCTTCAGTACAAGAGCGCTTGCTACTTCTTCAAGATAGATAAAGAGCATATTATTGATGCCACATGCAAGGGAGGGATTGCTCGTTTTGTCAACCATTCCTGCTCGCCAAATTGCGTCGCCAAAGTGATGTCTGTGAGGAATGAAAAGAAGGTGGTATTTTTCGCGGAGCGGGACATATTCCCCGGAGAAGAGATTACATATGACTACCACTTCAACCATGAGGATGAAGGTAAGAAGATTCCATGTTTTTGCAATTCAAAGAATTGCAGGCGCTACTTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD_2 SET zf-HC5HC2H_2

Protein Analysis

2075

Amino Acids

225.75

Weight (kDa)

8.87

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 260 - 306 1.7e-13 Tify domain binding domain
PHD_2 PF13831 1610 - 1642 1.5e-09 PHD-finger
zf-HC5HC2H_2 PF13832 1733 - 1809 4.3e-15 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 1740 - 1828 6.2e-11 PHD-like zinc-binding domain
SET PF00856 1941 - 2049 3.3e-24 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 426, 2452, 2774
AasI GACNNNNNNGTC 1 cut(s) 40
AatII GACGTC 1 cut(s) 876
Acc16I TGCGCA 1 cut(s) 3971
Acc36I ACCTGC 3 cut(s) 426, 2452, 2774
Acc65I GGTACC 2 cut(s) 305, 353
AccB1I GGYRCC 7 cut(s) 305, 353, 1484, 4034, 4761, 4976, 5253
AccB7I CCANNNNNTGG 2 cut(s) 706, 4031
AccBSI CCGCTC 2 cut(s) 585, 6109
AccI GTMKAC 1 cut(s) 5353
AccII CGCG 1 cut(s) 6104
AclI AACGTT 1 cut(s) 1455
AclWI GGATC 5 cut(s) 155, 2174, 2362, 4616, 4629
AcoI YGGCCR 4 cut(s) 519, 1687, 2967, 5509
AcyI GRCGYC 1 cut(s) 873
AfeI AGCGCT 3 cut(s) 607, 4992, 5956
AflII CTTAAG 2 cut(s) 1489, 3623
AflIII ACRYGT 5 cut(s) 533, 816, 2028, 3752, 5832
AhdI GACNNNNNGTC 1 cut(s) 3481
AhlI ACTAGT 3 cut(s) 749, 1982, 3293
AjiI CACGTC 2 cut(s) 3753, 5835
AjnI CCWGG 9 cut(s) 516, 1824, 1830, 2547, 2704, 3393, 4270, 4384, 5242
AjuI GAANNNNNNNTTGG 4 cut(s) 2574, 2606, 4698, 4730
AloI GAACNNNNNNTCC 2 cut(s) 5628, 5660
Alw21I GWGCWC 5 cut(s) 11, 1980, 4451, 5299, 5404
Alw44I GTGCAC 1 cut(s) 5295
AlwI GGATC 5 cut(s) 155, 2174, 2362, 4616, 4629
AlwNI CAGNNNCTG 1 cut(s) 995
Ama87I CYCGRG 1 cut(s) 865
Aor51HI AGCGCT 3 cut(s) 607, 4992, 5956
ApaLI GTGCAC 1 cut(s) 5295
AseI ATTAAT 1 cut(s) 5679
Asp700I GAANNNNTTC 1 cut(s) 2266
Asp718I GGTACC 2 cut(s) 305, 353
AspLEI GCGC 7 cut(s) 608, 1111, 3972, 4993, 5558, 5957, 6213
AsuC2I CCSGG 5 cut(s) 866, 867, 951, 1993, 6123
AsuII TTCGAA 1 cut(s) 3338
AsuNHI GCTAGC 1 cut(s) 2711
AvaI CYCGRG 1 cut(s) 865
AvaII GGWCC 7 cut(s) 514, 1828, 2249, 3235, 3767, 4342, 4929
AxyI CCTNAGG 1 cut(s) 5649
BaeGI GKGCMC 3 cut(s) 1274, 5258, 5299
BaeI ACNNNNGTAYC 4 cut(s) 2403, 2436, 3282, 3315
BalI TGGCCA 3 cut(s) 521, 1689, 5511
BamHI GGATCC 1 cut(s) 4621
BanI GGYRCC 7 cut(s) 305, 353, 1484, 4034, 4761, 4976, 5253
BanII GRGCYC 3 cut(s) 11, 572, 1980
BauI CACGAG 2 cut(s) 10, 729
BbsI GAAGAC 2 cut(s) 3271, 3927
Bbv12I GWGCWC 5 cut(s) 11, 1980, 4451, 5299, 5404
BbvCI CCTCAGC 3 cut(s) 1520, 5122, 5433
BccI CCATC 9 cut(s) 76, 1094, 1307, 1475, 1733, 2372, 3319, 3865, 5585
BceAI ACGGC 1 cut(s) 5565
BcgI CGANNNNNNTGC 2 cut(s) 5726, 5760
BciT130I CCWGG 9 cut(s) 518, 1826, 1832, 2549, 2706, 3395, 4272, 4386, 5244
BcnI CCSGG 5 cut(s) 866, 867, 951, 1993, 6123
BcuI ACTAGT 3 cut(s) 749, 1982, 3293
BfmI CTRYAG 7 cut(s) 503, 609, 1666, 2047, 2716, 2956, 4164
BfoI RGCGCY 4 cut(s) 609, 4994, 5958, 6214
BfrI CTTAAG 2 cut(s) 1489, 3623
BfuAI ACCTGC 3 cut(s) 426, 2452, 2774
BglI GCCNNNNNGGC 1 cut(s) 1004
Bme18I GGWCC 7 cut(s) 514, 1828, 2249, 3235, 3767, 4342, 4929
BmeRI GACNNNNNGTC 1 cut(s) 3481
BmeT110I CYCGRG 1 cut(s) 865
BmgBI CACGTC 2 cut(s) 3753, 5835
BmrI ACTGGG 2 cut(s) 901, 3729
BmtI GCTAGC 1 cut(s) 2715
BmuI ACTGGG 2 cut(s) 901, 3729
BpiI GAAGAC 2 cut(s) 3271, 3927
BplI GAGNNNNNCTC 6 cut(s) 2852, 2884, 3695, 3727, 4469, 4501
BpmI CTGGAG 6 cut(s) 454, 1089, 2490, 2549, 4293, 4859
Bpu10I CCTNAGC 4 cut(s) 1520, 4307, 5122, 5433
Bpu14I TTCGAA 1 cut(s) 3338
BpuEI CTTGAG 5 cut(s) 601, 1190, 2131, 3583, 4971
BpuMI CCSGG 5 cut(s) 866, 867, 951, 1993, 6123
BsaAI YACGTR 1 cut(s) 3956
BsaBI GATNNNNATC 2 cut(s) 2826, 4356
BsaHI GRCGYC 1 cut(s) 873
BsaI GGTCTC 2 cut(s) 550, 890
BsaWI WCCGGW 1 cut(s) 356
BsaXI ACNNNNNCTCC 5 cut(s) 26, 3640, 3670, 5871, 5901
Bse118I RCCGGY 1 cut(s) 3382
Bse21I CCTNAGG 1 cut(s) 5649
Bse3DI GCAATG 5 cut(s) 97, 900, 4763, 5110, 5216
Bse8I GATNNNNATC 2 cut(s) 2826, 4356
BseBI CCWGG 9 cut(s) 518, 1826, 1832, 2549, 2706, 3395, 4272, 4386, 5244
BseJI GATNNNNATC 2 cut(s) 2826, 4356
BseMI GCAATG 5 cut(s) 97, 900, 4763, 5110, 5216
BseSI GKGCMC 3 cut(s) 1274, 5258, 5299
BseYI CCCAGC 5 cut(s) 1724, 3440, 3670, 3674, 4625
BsgI GTGCAG 1 cut(s) 5380
Bsh1236I CGCG 1 cut(s) 6104
BshNI GGYRCC 7 cut(s) 305, 353, 1484, 4034, 4761, 4976, 5253
BsiHKAI GWGCWC 5 cut(s) 11, 1980, 4451, 5299, 5404
BsiHKCI CYCGRG 1 cut(s) 865
BsiSI CCGG 7 cut(s) 357, 866, 950, 1993, 3383, 4927, 6123
BslFI GGGAC 7 cut(s) 446, 884, 983, 3741, 4328, 5501, 6125
BsmBI CGTCTC 1 cut(s) 5841
BsmFI GGGAC 7 cut(s) 446, 884, 983, 3741, 4328, 5501, 6125
BsmI GAATGC 4 cut(s) 177, 841, 2394, 4787
Bso31I GGTCTC 2 cut(s) 550, 890
BsoBI CYCGRG 1 cut(s) 865
Bsp119I TTCGAA 1 cut(s) 3338
Bsp1286I GDGCHC 8 cut(s) 11, 572, 1274, 1980, 4451, 5258, 5299, 5404
Bsp1407I TGTACA 2 cut(s) 1156, 5799
Bsp19I CCATGG 2 cut(s) 3049, 4425
BspFNI CGCG 1 cut(s) 6104
BspMAI CTGCAG 4 cut(s) 507, 613, 1670, 2960
BspMI ACCTGC 3 cut(s) 426, 2452, 2774
BspOI GCTAGC 1 cut(s) 2715
BspPI GGATC 5 cut(s) 155, 2174, 2362, 4616, 4629
BspQI GCTCTTC 1 cut(s) 2605
BspT104I TTCGAA 1 cut(s) 3338
BspT107I GGYRCC 7 cut(s) 305, 353, 1484, 4034, 4761, 4976, 5253
BspTI CTTAAG 2 cut(s) 1489, 3623
BspTNI GGTCTC 2 cut(s) 550, 890
BsrBI CCGCTC 2 cut(s) 585, 6109
BsrDI GCAATG 5 cut(s) 97, 900, 4763, 5110, 5216
BsrFI RCCGGY 1 cut(s) 3382
BsrGI TGTACA 2 cut(s) 1156, 5799
BssAI RCCGGY 1 cut(s) 3382
BssNAI GTATAC 1 cut(s) 5354
BssNI GRCGYC 1 cut(s) 873
BssSI CACGAG 2 cut(s) 10, 729
BssT1I CCWWGG 5 cut(s) 522, 3049, 4425, 5393, 5821
Bst1107I GTATAC 1 cut(s) 5354
Bst2BI CACGAG 2 cut(s) 10, 729
Bst2UI CCWGG 9 cut(s) 518, 1826, 1832, 2549, 2706, 3395, 4272, 4386, 5244
Bst6I CTCTTC 3 cut(s) 1701, 2605, 6123
BstACI GRCGYC 1 cut(s) 873
BstAFI CTTAAG 2 cut(s) 1489, 3623
BstAPI GCANNNNNTGC 3 cut(s) 418, 1933, 4923
BstAUI TGTACA 2 cut(s) 1156, 5799
BstBAI YACGTR 1 cut(s) 3956
BstBI TTCGAA 1 cut(s) 3338
BstDSI CCRYGG 3 cut(s) 1756, 3049, 4425
BstENI CCTNNNNNAGG 4 cut(s) 521, 1829, 2105, 4310
BstFNI CGCG 1 cut(s) 6104
BstH2I RGCGCY 4 cut(s) 609, 4994, 5958, 6214
BstHHI GCGC 7 cut(s) 608, 1111, 3972, 4993, 5558, 5957, 6213
BstNI CCWGG 9 cut(s) 518, 1826, 1832, 2549, 2706, 3395, 4272, 4386, 5244
BstSFI CTRYAG 7 cut(s) 503, 609, 1666, 2047, 2716, 2956, 4164
BstSLI GKGCMC 3 cut(s) 1274, 5258, 5299
BstUI CGCG 1 cut(s) 6104
BstV2I GAAGAC 2 cut(s) 3271, 3927
BstX2I RGATCY 3 cut(s) 160, 2354, 4621
BstXI CCANNNNNNTGG 2 cut(s) 2231, 4700
BstYI RGATCY 3 cut(s) 160, 2354, 4621
BstZ17I GTATAC 1 cut(s) 5354
Bsu36I CCTNAGG 1 cut(s) 5649
BtgI CCRYGG 3 cut(s) 1756, 3049, 4425
BtrI CACGTC 2 cut(s) 3753, 5835
BtsI GCAGTG 4 cut(s) 1675, 2182, 5635, 5860
BveI ACCTGC 3 cut(s) 426, 2452, 2774
CaiI CAGNNNCTG 1 cut(s) 995
CfoI GCGC 7 cut(s) 608, 1111, 3972, 4993, 5558, 5957, 6213
Cfr10I RCCGGY 1 cut(s) 3382
Cfr9I CCCGGG 1 cut(s) 865
CseI GACGC 2 cut(s) 308, 6043
CsiI ACCWGGT 2 cut(s) 1824, 2704
CspCI CAANNNNNGTGG 4 cut(s) 127, 162, 1581, 1616
DraI TTTAAA 1 cut(s) 3591
DrdI GACNNNNNNGTC 1 cut(s) 40
DriI GACNNNNNGTC 1 cut(s) 3481
DseDI GACNNNNNNGTC 1 cut(s) 40
EaeI YGGCCR 4 cut(s) 519, 1687, 2967, 5509
Eam1104I CTCTTC 3 cut(s) 1701, 2605, 6123
Eam1105I GACNNNNNGTC 1 cut(s) 3481
EarI CTCTTC 3 cut(s) 1701, 2605, 6123
EciI GGCGGA 1 cut(s) 441
Ecl136II GAGCTC 2 cut(s) 9, 1978
Eco130I CCWWGG 5 cut(s) 522, 3049, 4425, 5393, 5821
Eco24I GRGCYC 3 cut(s) 11, 572, 1980
Eco31I GGTCTC 2 cut(s) 550, 890
Eco47I GGWCC 7 cut(s) 514, 1828, 2249, 3235, 3767, 4342, 4929
Eco47III AGCGCT 3 cut(s) 607, 4992, 5956
Eco53kI GAGCTC 2 cut(s) 9, 1978
Eco81I CCTNAGG 1 cut(s) 5649
Eco88I CYCGRG 1 cut(s) 865
EcoICRI GAGCTC 2 cut(s) 9, 1978
EcoNI CCTNNNNNAGG 4 cut(s) 521, 1829, 2105, 4310
EcoO109I RGGNCCY 3 cut(s) 3767, 3888, 4342
EcoRI GAATTC 5 cut(s) 508, 931, 1138, 2124, 2614
EcoRII CCWGG 9 cut(s) 516, 1824, 1830, 2547, 2704, 3393, 4270, 4384, 5242
EcoT14I CCWWGG 5 cut(s) 522, 3049, 4425, 5393, 5821
EcoT22I ATGCAT 3 cut(s) 1771, 2396, 4789
EcoT38I GRGCYC 3 cut(s) 11, 572, 1980
ErhI CCWWGG 5 cut(s) 522, 3049, 4425, 5393, 5821
Esp3I CGTCTC 1 cut(s) 5841
FalI AAGNNNNNCTT 6 cut(s) 556, 588, 908, 940, 1616, 1648
FaqI GGGAC 7 cut(s) 446, 884, 983, 3741, 4328, 5501, 6125
FauI CCCGC 5 cut(s) 578, 3494, 5176, 5858, 6102
FauNDI CATATG 3 cut(s) 1053, 5220, 6139
FblI GTMKAC 1 cut(s) 5353
FriOI GRGCYC 3 cut(s) 11, 572, 1980
FspI TGCGCA 1 cut(s) 3971
GlaI GCGC 7 cut(s) 607, 1110, 3971, 4992, 5557, 5956, 6212
GsaI CCCAGC 5 cut(s) 1728, 3444, 3674, 3678, 4629
GsuI CTGGAG 6 cut(s) 454, 1089, 2490, 2549, 4293, 4859
HaeII RGCGCY 4 cut(s) 609, 4994, 5958, 6214
HapII CCGG 7 cut(s) 357, 866, 950, 1993, 3383, 4927, 6123
HgaI GACGC 2 cut(s) 308, 6043
HhaI GCGC 7 cut(s) 608, 1111, 3972, 4993, 5558, 5957, 6213
Hin1I GRCGYC 1 cut(s) 873
Hin6I GCGC 7 cut(s) 606, 1109, 3970, 4991, 5556, 5955, 6211
HinP1I GCGC 7 cut(s) 606, 1109, 3970, 4991, 5556, 5955, 6211
HindIII AAGCTT 4 cut(s) 3947, 4712, 5028, 5939
HpaI GTTAAC 3 cut(s) 2466, 2761, 3412
HpaII CCGG 7 cut(s) 357, 866, 950, 1993, 3383, 4927, 6123
Hpy99I CGWCG 2 cut(s) 878, 6059
HpyCH4IV ACGT 9 cut(s) 873, 1338, 1455, 1588, 3752, 3955, 4513, 5587, 5834
HpySE526I ACGT 9 cut(s) 873, 1338, 1455, 1588, 3752, 3955, 4513, 5587, 5834
Hsp92I GRCGYC 1 cut(s) 873
HspAI GCGC 7 cut(s) 606, 1109, 3970, 4991, 5556, 5955, 6211
KpnI GGTACC 2 cut(s) 309, 357
KroI GCCGGC 1 cut(s) 3382
KroNI GCCGGC 1 cut(s) 3384
KspAI GTTAAC 3 cut(s) 2466, 2761, 3412
LguI GCTCTTC 1 cut(s) 2605
LmnI GCTCC 9 cut(s) 67, 428, 473, 641, 1873, 3633, 4454, 5653, 6106
MabI ACCWGGT 2 cut(s) 1824, 2704
MaeII ACGT 9 cut(s) 873, 1338, 1455, 1588, 3752, 3955, 4513, 5587, 5834
MbiI CCGCTC 2 cut(s) 585, 6109
MfeI CAATTG 4 cut(s) 1816, 2021, 3402, 4556
MflI RGATCY 3 cut(s) 160, 2354, 4621
MhlI GDGCHC 8 cut(s) 11, 572, 1274, 1980, 4451, 5258, 5299, 5404
MlsI TGGCCA 3 cut(s) 521, 1689, 5511
MluNI TGGCCA 3 cut(s) 521, 1689, 5511
MlyI GAGTC 8 cut(s) 1352, 2391, 2440, 3704, 3892, 4713, 4978, 5233
MmeI TCCRAC 4 cut(s) 766, 2677, 2986, 5726
Mox20I TGGCCA 3 cut(s) 521, 1689, 5511
Mph1103I ATGCAT 3 cut(s) 1771, 2396, 4789
MroNI GCCGGC 1 cut(s) 3382
MroXI GAANNNNTTC 1 cut(s) 2266
MscI TGGCCA 3 cut(s) 521, 1689, 5511
MslI CAYNNNNRTG 4 cut(s) 3081, 3309, 5679, 6162
Msp20I TGGCCA 3 cut(s) 521, 1689, 5511
MspA1I CMGCKG 8 cut(s) 1090, 1583, 2211, 2955, 3674, 4195, 5126, 5183
MspCI CTTAAG 2 cut(s) 1489, 3623
MspI CCGG 7 cut(s) 357, 866, 950, 1993, 3383, 4927, 6123
MunI CAATTG 4 cut(s) 1816, 2021, 3402, 4556
Mva1269I GAATGC 4 cut(s) 177, 841, 2394, 4787
MvaI CCWGG 9 cut(s) 518, 1826, 1832, 2549, 2706, 3395, 4272, 4386, 5244
MvnI CGCG 1 cut(s) 6104
NaeI GCCGGC 1 cut(s) 3384
NciI CCSGG 5 cut(s) 866, 867, 951, 1993, 6123
NcoI CCATGG 2 cut(s) 3049, 4425
NdeI CATATG 3 cut(s) 1053, 5220, 6139
NgoMIV GCCGGC 1 cut(s) 3382
NheI GCTAGC 1 cut(s) 2711
NmuCI GTSAC 7 cut(s) 249, 373, 529, 536, 1018, 2683, 5000
NsbI TGCGCA 1 cut(s) 3971
NsiI ATGCAT 3 cut(s) 1771, 2396, 4789
NspV TTCGAA 1 cut(s) 3338
PaeI GCATGC 2 cut(s) 1931, 5056
PaqCI CACCTGC 3 cut(s) 426, 2452, 2774
PciI ACATGT 3 cut(s) 533, 816, 2028
PciSI GCTCTTC 1 cut(s) 2605
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PctI GAATGC 4 cut(s) 177, 841, 2394, 4787
PdiI GCCGGC 1 cut(s) 3384
PdmI GAANNNNTTC 1 cut(s) 2266
PflFI GACNNNGTC 2 cut(s) 542, 3901
PflMI CCANNNNNTGG 2 cut(s) 706, 4031
PfoI TCCNGGA 3 cut(s) 949, 1830, 5242
PleI GAGTC 8 cut(s) 1351, 2390, 2439, 3704, 3892, 4713, 4978, 5233
PpsI GAGTC 8 cut(s) 1351, 2390, 2439, 3704, 3892, 4713, 4978, 5233
Ppu21I YACGTR 1 cut(s) 3956
PpuMI RGGWCCY 2 cut(s) 3767, 4342
PscI ACATGT 3 cut(s) 533, 816, 2028
PshBI ATTAAT 1 cut(s) 5679
Psp124BI GAGCTC 2 cut(s) 11, 1980
Psp1406I AACGTT 1 cut(s) 1455
Psp5II RGGWCCY 2 cut(s) 3767, 4342
Psp6I CCWGG 9 cut(s) 516, 1824, 1830, 2547, 2704, 3393, 4270, 4384, 5242
PspFI CCCAGC 5 cut(s) 1724, 3440, 3670, 3674, 4625
PspGI CCWGG 9 cut(s) 516, 1824, 1830, 2547, 2704, 3393, 4270, 4384, 5242
PspPPI RGGWCCY 2 cut(s) 3767, 4342
PsrI GAACNNNNNNTAC 2 cut(s) 4837, 4869
PstI CTGCAG 4 cut(s) 507, 613, 1670, 2960
PstNI CAGNNNCTG 1 cut(s) 995
PsuI RGATCY 3 cut(s) 160, 2354, 4621
PsyI GACNNNGTC 2 cut(s) 542, 3901
PvuII CAGCTG 6 cut(s) 1583, 2211, 2955, 3674, 4195, 5126
RseI CAYNNNNRTG 4 cut(s) 3081, 3309, 5679, 6162
SacI GAGCTC 2 cut(s) 11, 1980
SapI GCTCTTC 1 cut(s) 2605
SchI GAGTC 8 cut(s) 1352, 2391, 2440, 3704, 3892, 4713, 4978, 5233
SduI GDGCHC 8 cut(s) 11, 572, 1274, 1980, 4451, 5258, 5299, 5404
SexAI ACCWGGT 2 cut(s) 1824, 2704
SfcI CTRYAG 7 cut(s) 503, 609, 1666, 2047, 2716, 2956, 4164
SfuI TTCGAA 1 cut(s) 3338
SinI GGWCC 7 cut(s) 514, 1828, 2249, 3235, 3767, 4342, 4929
SmaI CCCGGG 1 cut(s) 867
SmiI ATTTAAAT 1 cut(s) 3591
SmiMI CAYNNNNRTG 4 cut(s) 3081, 3309, 5679, 6162
SmlI CTYRAG 7 cut(s) 580, 1205, 1489, 2146, 3562, 3623, 4986
SmoI CTYRAG 7 cut(s) 580, 1205, 1489, 2146, 3562, 3623, 4986
SpeI ACTAGT 3 cut(s) 749, 1982, 3293
SphI GCATGC 2 cut(s) 1931, 5056
SspI AATATT 5 cut(s) 649, 1513, 3361, 4945, 5455
SstI GAGCTC 2 cut(s) 11, 1980
StyI CCWWGG 5 cut(s) 522, 3049, 4425, 5393, 5821
SwaI ATTTAAAT 1 cut(s) 3591
TaiI ACGT 9 cut(s) 876, 1341, 1458, 1591, 3755, 3958, 4516, 5590, 5837
TaqI TCGA 5 cut(s) 32, 2537, 2575, 3338, 4103
TaqII GACCGA 1 cut(s) 4240
TatI WGTACW 6 cut(s) 166, 1156, 1940, 2197, 5799, 5946
TauI GCSGC 1 cut(s) 2969
TseFI GTSAC 7 cut(s) 249, 373, 529, 536, 1018, 2683, 5000
Tsp45I GTSAC 7 cut(s) 249, 373, 529, 536, 1018, 2683, 5000
TspGWI ACGGA 2 cut(s) 171, 3819
TspMI CCCGGG 1 cut(s) 865
Tth111I GACNNNGTC 2 cut(s) 542, 3901
Van91I CCANNNNNTGG 2 cut(s) 706, 4031
Vha464I CTTAAG 2 cut(s) 1489, 3623
VneI GTGCAC 1 cut(s) 5295
VpaK11BI GGWCC 7 cut(s) 514, 1828, 2249, 3235, 3767, 4342, 4929
VspI ATTAAT 1 cut(s) 5679
XagI CCTNNNNNAGG 4 cut(s) 521, 1829, 2105, 4310
XbaI TCTAGA 4 cut(s) 2258, 2453, 4328, 4750
XcmI CCANNNNNNNNNTGG 1 cut(s) 1822
XmaI CCCGGG 1 cut(s) 865
XmiI GTMKAC 1 cut(s) 5353
XmnI GAANNNNTTC 1 cut(s) 2266
ZraI GACGTC 1 cut(s) 874
Zsp2I ATGCAT 3 cut(s) 1771, 2396, 4789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.