pycom15g12850

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008755
Physical Location & Seq
Reverse (-)
8805096 .. 8807007
1912 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
ATGGCTGCTGCTTGGGCTTCAACACAGTGTTTCAACTCTCGCCTCCTCCCACCGCCGGATACTTGTCGTTCCCACGGAGGCAGAGGCAGCAGCAGCATTCAACGCCTTCTCCTGTCGTCTCAGATTCCCAAATCCCTCTCTCTTTCTTCGTCGCCATCTTCCTCTTCGTTTTCCATAGGAGGAGGAGGTGCAATTGAGAGGAAGGAGCGTTCGTCTTTTGCTCAGACAGCGGCTGTTCGCCATCTCGTGGGTTCCGTCACCAAAACTCAGGGCCTCCGTTTCGCAGTGGTTGTGGCGCGGTTCAATGAGTTTATAACAAAGCAGCTGTTAGAGGGAGCTCTGACCACTTTCAGCAACTACTCAATTCAAGACGAGGATGTTGACGTTGTGTGGGTTCCTGGTAGTTTTGAAATCGGTGTAACTGCACAGAGGCTTGCAAGTTTGGGAAAATATCATGCAATTCTATGCATCGGAGCTGTGATTAGAGGTGATACAACCCATTATGACGCGGTTGCTAATTCAGCAGCATCCGGAGTACTTTCAGCCGGATTACAATCAGGAGTGCCGTGCGTATTTGGTGTCCTAACTTGTGATGATATGGAGCAGGCTATCAATCGAGCGGGTGGTAAATCTGGAAATAAGGGTGCAGAGACTGCTATGACAGCTATTGAGATGGCATCTTTGTTTGAGCACCAACTGAAGTAA

Protein Analysis

235

Amino Acids

24.54

Weight (kDa)

8.31

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 91 - 229 9e-52 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 247
AccBSI CCGCTC 1 cut(s) 620
AccII CGCG 2 cut(s) 298, 509
AccIII TCCGGA 1 cut(s) 530
AciI CCGC 5 cut(s) 53, 230, 298, 509, 620
AfaI GTAC 1 cut(s) 537
AfiI CCNNNNNNNGG 2 cut(s) 55, 247
AgsI TTSAA 6 cut(s) 21, 34, 101, 304, 368, 410
AjnI CCWGG 1 cut(s) 397
AloI GAACNNNNNNTCC 2 cut(s) 193, 225
AluBI AGCT 4 cut(s) 325, 338, 476, 665
AluI AGCT 4 cut(s) 325, 338, 476, 665
Alw21I GWGCWC 2 cut(s) 340, 693
Alw26I GTCTC 2 cut(s) 123, 644
AlwNI CAGNNNCTG 2 cut(s) 233, 653
Aor13HI TCCGGA 1 cut(s) 530
AoxI GGCC 1 cut(s) 271
ApeKI GCWGC 7 cut(s) 5, 8, 87, 90, 93, 322, 524
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 271
AsuHPI GGTGA 2 cut(s) 250, 500
BanII GRGCYC 1 cut(s) 340
BauI CACGAG 1 cut(s) 245
Bbv12I GWGCWC 2 cut(s) 340, 693
BbvI GCAGC 5 cut(s) 99, 102, 105, 334, 536
BccI CCATC 3 cut(s) 163, 249, 667
BceAI ACGGC 1 cut(s) 550
BciT130I CCWGG 1 cut(s) 399
BciVI GTATCC 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 123, 644
BfuI GTATCC 1 cut(s) 52
BisI GCNGC 8 cut(s) 6, 9, 88, 91, 94, 231, 323, 525
BlsI GCNGC 8 cut(s) 7, 10, 89, 92, 95, 232, 324, 526
BmcAI AGTACT 1 cut(s) 537
Bme1390I CCNGG 1 cut(s) 399
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 2 cut(s) 253, 396
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 3 cut(s) 477, 536, 686
BsaBI GATNNNNATC 1 cut(s) 553
BsaJI CCNNGG 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 530
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 197, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 247
Bse8I GATNNNNATC 1 cut(s) 553
BseAI TCCGGA 1 cut(s) 530
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 2 cut(s) 382, 527
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 2 cut(s) 55, 247
BseMII CTCAG 3 cut(s) 134, 236, 281
BseRI GAGGAG 3 cut(s) 35, 195, 198
BseXI GCAGC 5 cut(s) 99, 102, 105, 334, 536
BsgI GTGCAG 2 cut(s) 408, 666
Bsh1236I CGCG 2 cut(s) 298, 509
BshFI GGCC 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 340, 693
BsiSI CCGG 3 cut(s) 56, 531, 546
BslI CCNNNNNNNGG 2 cut(s) 55, 247
BsmAI GTCTC 2 cut(s) 123, 644
BsmBI CGTCTC 1 cut(s) 123
BsmI GAATGC 1 cut(s) 96
BsnI GGCC 1 cut(s) 273
Bsp1286I GDGCHC 2 cut(s) 340, 693
Bsp13I TCCGGA 1 cut(s) 530
BspACI CCGC 5 cut(s) 53, 230, 298, 509, 620
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 3 cut(s) 133, 235, 280
BspEI TCCGGA 1 cut(s) 530
BspFNI CGCG 2 cut(s) 298, 509
BspLI GGNNCC 2 cut(s) 253, 396
BsrBI CCGCTC 1 cut(s) 620
BssECI CCNNGG 1 cut(s) 73
BssSI CACGAG 1 cut(s) 245
Bst2BI CACGAG 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 27
Bst6I CTCTTC 1 cut(s) 169
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 2 cut(s) 435, 606
BstDEI CTNAG 3 cut(s) 120, 222, 267
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 2 cut(s) 382, 527
BstFNI CGCG 2 cut(s) 298, 509
BstHHI GCGC 1 cut(s) 298
BstMAI GTCTC 2 cut(s) 123, 644
BstMWI GCNNNNNNNGC 8 cut(s) 14, 87, 93, 102, 227, 521, 653, 662
BstNI CCWGG 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 397
BstUI CGCG 2 cut(s) 298, 509
BstV1I GCAGC 5 cut(s) 99, 102, 105, 334, 536
BsuI GTATCC 1 cut(s) 52
BsuRI GGCC 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 2 cut(s) 382, 527
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 2 cut(s) 32, 291
Cac8I GCNNGC 2 cut(s) 435, 606
CaiI CAGNNNCTG 2 cut(s) 233, 653
CfoI GCGC 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 271
CseI GACGC 1 cut(s) 515
Csp6I GTAC 1 cut(s) 536
CviAII CATG 1 cut(s) 455
CviQI GTAC 1 cut(s) 536
DdeI CTNAG 3 cut(s) 120, 222, 267
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 340
Eco53kI GAGCTC 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 397
EcoT22I ATGCAT 1 cut(s) 470
EcoT38I GRGCYC 1 cut(s) 340
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 1 cut(s) 458
FaiI YATR 7 cut(s) 176, 314, 456, 466, 504, 599, 659
FatI CATG 1 cut(s) 454
FauI CCCGC 1 cut(s) 613
Fnu4HI GCNGC 8 cut(s) 6, 9, 88, 91, 94, 231, 323, 525
FokI GGATG 2 cut(s) 389, 514
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 8 cut(s) 6, 9, 88, 91, 94, 231, 323, 525
GlaI GCGC 1 cut(s) 297
GluI GCNGC 8 cut(s) 6, 9, 88, 91, 94, 231, 323, 525
HaeIII GGCC 1 cut(s) 273
HapII CCGG 3 cut(s) 56, 531, 546
HgaI GACGC 1 cut(s) 515
HhaI GCGC 1 cut(s) 298
Hin1II CATG 1 cut(s) 458
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 1 cut(s) 124
HpaII CCGG 3 cut(s) 56, 531, 546
HphI GGTGA 2 cut(s) 250, 500
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 123, 225, 342, 473
Hpy188III TCNNGA 4 cut(s) 368, 531, 558, 633
Hpy8I GTNNAC 1 cut(s) 382
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 116, 196
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 6 cut(s) 191, 425, 437, 458, 468, 647
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 87, 93, 102, 227, 521, 653, 662
HpyF3I CTNAG 3 cut(s) 120, 222, 267
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 1 cut(s) 458
HspAI GCGC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 530
LmnI GCTCC 4 cut(s) 205, 335, 473, 601
Lsp1109I GCAGC 5 cut(s) 99, 102, 105, 334, 536
LweI GCATC 3 cut(s) 477, 536, 686
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 2 cut(s) 256, 418
MbiI CCGCTC 1 cut(s) 620
MboII GAAGA 3 cut(s) 138, 150, 156
MfeI CAATTG 1 cut(s) 192
MhlI GDGCHC 2 cut(s) 340, 693
MluCI AATT 4 cut(s) 192, 363, 459, 517
Mph1103I ATGCAT 1 cut(s) 470
MroI TCCGGA 1 cut(s) 530
MspA1I CMGCKG 2 cut(s) 230, 325
MspI CCGG 3 cut(s) 56, 531, 546
MspR9I CCNGG 1 cut(s) 399
MunI CAATTG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 96
MvaI CCWGG 1 cut(s) 399
MvnI CGCG 2 cut(s) 298, 509
MwoI GCNNNNNNNGC 8 cut(s) 14, 87, 93, 102, 227, 521, 653, 662
NlaIII CATG 1 cut(s) 458
NlaIV GGNNCC 2 cut(s) 253, 396
NmuCI GTSAC 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 470
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 1 cut(s) 96
PfeI GAWTC 1 cut(s) 124
PflMI CCANNNNNTGG 1 cut(s) 247
PkrI GCNGC 8 cut(s) 7, 10, 89, 92, 95, 232, 324, 526
PsiI TTATAA 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 340
Psp6I CCWGG 1 cut(s) 397
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 2 cut(s) 253, 396
PspPI GGNCC 1 cut(s) 271
PstNI CAGNNNCTG 2 cut(s) 233, 653
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
SacI GAGCTC 1 cut(s) 340
SatI GCNGC 8 cut(s) 6, 9, 88, 91, 94, 231, 323, 525
Sau96I GGNCC 1 cut(s) 271
ScaI AGTACT 1 cut(s) 537
ScrFI CCNGG 1 cut(s) 399
SduI GDGCHC 2 cut(s) 340, 693
SetI ASST 7 cut(s) 190, 327, 340, 387, 478, 490, 667
SfaNI GCATC 3 cut(s) 477, 536, 686
Sse9I AATT 4 cut(s) 192, 363, 459, 517
SsiI CCGC 5 cut(s) 53, 230, 298, 509, 620
SstI GAGCTC 1 cut(s) 340
StyD4I CCNGG 1 cut(s) 397
TaaI ACNGT 1 cut(s) 27
TaiI ACGT 1 cut(s) 387
TaqI TCGA 1 cut(s) 616
TasI AATT 4 cut(s) 192, 363, 459, 517
TatI WGTACW 1 cut(s) 535
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 1 cut(s) 124
TscAI CASTG 2 cut(s) 32, 291
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 7 cut(s) 5, 8, 87, 90, 93, 322, 524
Tsp45I GTSAC 1 cut(s) 256
TspGWI ACGGA 3 cut(s) 90, 244, 266
TspRI CASTG 2 cut(s) 32, 291
Van91I CCANNNNNTGG 1 cut(s) 247
ZrmI AGTACT 1 cut(s) 537
Zsp2I ATGCAT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.