pycom15g13130

Belongs to the eukaryotic ribosomal protein eS24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004025
Physical Location & Seq
Forward (+)
8996621 .. 8998270
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
ATGGCGGATAGCAAGGCGGTGACGATCCGGACGAGGAAGTTCATGACCAACCGTCTCCTCTCCAGAAAGCAGTTCGTTATCGATGTTCTGCATCCAGGAAGACCGAATGTTTCCAAGGCGGAGTTGAAGGAGAAACTGGCAAGGTTGTATGAAGTGAGGGATCCAGATGCCATCTTTGTTTTCAAGTTCCGCACCCATTTTGGAGGAGGGAAGTGCACTGGTTTTGGTTTGATTTATGATTCTGTTGAGAATGCAAAGAAGTACGAGCCCAAGTACAGGCTGATCAGGAATGGATTGGATACAAAGGTGGAGAAGTCAAGGAAACAACTCAAGGAAAGGAAGAACAGAGCCAAGAAGATCCGCGGAGTAAAGAAGACTAAGGCTGGTGATGCTGCCAAGGGCGGGAAGAAGAAATGA

Protein Analysis

139

Amino Acids

15.84

Weight (kDa)

10.54

Isoelectric Point (pI)

13.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S24e PF01282 7 - 98 1.5e-38 Ribosomal protein S24e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 363
AccIII TCCGGA 1 cut(s) 27
AciI CCGC 7 cut(s) 5, 17, 119, 190, 361, 363, 402
AclWI GGATC 4 cut(s) 19, 155, 168, 352
AfaI GTAC 2 cut(s) 263, 275
AfiI CCNNNNNNNGG 2 cut(s) 276, 402
AgsI TTSAA 2 cut(s) 127, 184
AhdI GACNNNNNGTC 1 cut(s) 51
AjnI CCWGG 1 cut(s) 94
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 1 cut(s) 59
Alw44I GTGCAC 1 cut(s) 214
AlwI GGATC 4 cut(s) 19, 155, 168, 352
Aor13HI TCCGGA 1 cut(s) 27
ApaLI GTGCAC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 392
AsuHPI GGTGA 2 cut(s) 31, 398
BaeGI GKGCMC 1 cut(s) 218
BaeI ACNNNNGTAYC 2 cut(s) 291, 324
BamHI GGATCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 106, 380
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 96
BciVI GTATCC 1 cut(s) 292
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 59
BfuI GTATCC 1 cut(s) 292
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
Bme1390I CCNGG 1 cut(s) 96
BmeRI GACNNNNNGTC 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 3 cut(s) 100, 157, 379
BpiI GAAGAC 2 cut(s) 106, 380
BpmI CTGGAG 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 314
Bsa29I ATCGAT 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 114, 361, 396
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 402
Bse1I ACTGG 2 cut(s) 141, 223
BseAI TCCGGA 1 cut(s) 27
BseBI CCWGG 1 cut(s) 96
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 114, 361, 396
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 2 cut(s) 276, 402
BseNI ACTGG 2 cut(s) 141, 223
BseRI GAGGAG 2 cut(s) 47, 219
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 1 cut(s) 379
Bsh1236I CGCG 1 cut(s) 363
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 218
BsiSI CCGG 1 cut(s) 28
BslI CCNNNNNNNGG 2 cut(s) 276, 402
BsmAI GTCTC 1 cut(s) 59
BsmBI CGTCTC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 218, 270
Bsp13I TCCGGA 1 cut(s) 27
Bsp143I GATC 4 cut(s) 24, 160, 282, 357
BspACI CCGC 7 cut(s) 5, 17, 119, 190, 361, 363, 402
BspDI ATCGAT 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 27
BspFNI CGCG 1 cut(s) 363
BspHI TCATGA 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 162
BspPI GGATC 4 cut(s) 19, 155, 168, 352
BsrI ACTGG 2 cut(s) 141, 223
BssECI CCNNGG 3 cut(s) 114, 361, 396
BssMI GATC 4 cut(s) 24, 160, 282, 357
BssT1I CCWWGG 2 cut(s) 114, 396
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 53
BstDEI CTNAG 1 cut(s) 378
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 1 cut(s) 91
BstFNI CGCG 1 cut(s) 363
BstKTI GATC 4 cut(s) 27, 163, 285, 360
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 4 cut(s) 24, 160, 282, 357
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 2 cut(s) 106, 380
BstX2I RGATCY 2 cut(s) 160, 357
BstYI RGATCY 2 cut(s) 160, 357
Bsu15I ATCGAT 1 cut(s) 81
BsuI GTATCC 1 cut(s) 292
BsuTUI ATCGAT 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 216
CciI TCATGA 1 cut(s) 42
Cfr42I CCGCGG 1 cut(s) 364
ClaI ATCGAT 1 cut(s) 81
Csp6I GTAC 2 cut(s) 262, 274
CviAII CATG 1 cut(s) 43
CviJI RGCY 4 cut(s) 268, 280, 350, 383
CviKI_1 RGCY 4 cut(s) 268, 280, 350, 383
CviQI GTAC 2 cut(s) 262, 274
DdeI CTNAG 1 cut(s) 378
DpnI GATC 4 cut(s) 26, 162, 284, 359
DpnII GATC 4 cut(s) 24, 160, 282, 357
DriI GACNNNNNGTC 1 cut(s) 51
Eam1105I GACNNNNNGTC 1 cut(s) 51
EciI GGCGGA 2 cut(s) 20, 134
Eco130I CCWWGG 2 cut(s) 114, 396
Eco24I GRGCYC 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 94
EcoT14I CCWWGG 2 cut(s) 114, 396
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 2 cut(s) 114, 396
Esp3I CGTCTC 1 cut(s) 59
FaeI CATG 1 cut(s) 46
FaiI YATR 3 cut(s) 44, 150, 237
FatI CATG 1 cut(s) 42
FauI CCCGC 1 cut(s) 395
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 1 cut(s) 78
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 1 cut(s) 393
GluI GCNGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 46
HapII CCGG 1 cut(s) 28
Hin1II CATG 1 cut(s) 46
HinfI GANTC 1 cut(s) 239
HpaII CCGG 1 cut(s) 28
HphI GGTGA 2 cut(s) 31, 398
Hpy166II GTNNAC 1 cut(s) 216
Hpy188III TCNNGA 5 cut(s) 28, 43, 63, 164, 286
Hpy8I GTNNAC 1 cut(s) 216
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4V TGCA 3 cut(s) 91, 216, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 1 cut(s) 378
Hsp92II CATG 1 cut(s) 46
Kpn2I TCCGGA 1 cut(s) 27
Ksp22I TGATCA 1 cut(s) 282
KspI CCGCGG 1 cut(s) 364
Kzo9I GATC 4 cut(s) 24, 160, 282, 357
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 3 cut(s) 100, 157, 379
MaeIII GTNAC 1 cut(s) 19
MalI GATC 4 cut(s) 26, 162, 284, 359
MboI GATC 4 cut(s) 24, 160, 282, 357
MboII GAAGA 4 cut(s) 111, 352, 367, 385
MflI RGATCY 2 cut(s) 160, 357
MhlI GDGCHC 2 cut(s) 218, 270
MnlI CCTC 5 cut(s) 27, 68, 150, 197, 200
MroI TCCGGA 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 363
MspI CCGG 1 cut(s) 28
MspR9I CCNGG 1 cut(s) 96
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 96
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 1 cut(s) 389
NdeII GATC 4 cut(s) 24, 160, 282, 357
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 19
PagI TCATGA 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 239
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 162
PsuI RGATCY 2 cut(s) 160, 357
RsaI GTAC 2 cut(s) 263, 275
RsaNI GTAC 2 cut(s) 262, 274
SacII CCGCGG 1 cut(s) 364
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 4 cut(s) 24, 160, 282, 357
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 2 cut(s) 218, 270
SetI ASST 2 cut(s) 146, 309
SfaNI GCATC 3 cut(s) 100, 157, 379
Sfr303I CCGCGG 1 cut(s) 364
SgrBI CCGCGG 1 cut(s) 364
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SsiI CCGC 7 cut(s) 5, 17, 119, 190, 361, 363, 402
StyD4I CCNGG 1 cut(s) 94
StyI CCWWGG 2 cut(s) 114, 396
TaaI ACNGT 1 cut(s) 53
TaqI TCGA 1 cut(s) 81
TaqII GACCGA 1 cut(s) 118
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 1 cut(s) 239
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 31, 165
TspRI CASTG 1 cut(s) 223
VneI GTGCAC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.