pycom15g16700

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009820
Physical Location & Seq
Forward (+)
11721903 .. 11725537
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2085 bp
ATGTTGGACGGGCTGCTGGGCCGTGGATTTGCCTCCAAATGCAAATCGCTGATTAAATCGACGAAGACTCGGATCGATGTGATACGGAGGAAGAGGAACGCAACGCAGAAGTTTCTGAAGAAGGATATCGCTGATCTGCTCACCAATGGCCTTGATATCAATGCTTTCGGAAGGGCTGATGGACTCACGGCGGAGTTAATCCTTTCTTCGTGTTATGATTTCGTTGAGCGATGCTGTGATTTGGTGCTCAAGAATCTTTCAGTTATGCAGAAAGAAAGTGATTTCCCTGAGGAATGTAAGGAACCTGTGTCATCGCTGATGATTGCTGCTGCAAGGTTCTCTGATTTACCAGAGTTGCGTGACCTTAGGCAACTTTTTCAAGAGAGATACGCGAATTCTCTGGAGTATTTTGTGAATCAAAAGTTTGTTGAGAACATAGCTTCAAGGCCTCCTACACTGGAGAAGAAGGTTCAGTTATTGAAAGACATAGCAGTAGAGTTCTCAATAAATTGGGACTCCAAGGCCTTTGAACAGAGGATGGCAAAACCTCCTCCTGCGTTTACACAGGACAGGCCAAAAACTTACGGGCCTTCCCATTTTAGTGATGATAAAAGCAAATTGTCTACTGGGAAATCTGCAGTCCCAAAAGAACATAATCTTTTACCCACAGAAAGGAGATTTGATCTTAATGATGCTCAGAAATTACACAAGGAGAAGCTCGGTACTATATTGAAAAAAGATGATCACGTTCCTCAAACGAGGCATAGACCCTTTGGGATAGAACATAAGTCTCTCAGTGCCAGGGAAGATACTGTCATGGAAAAAGATGGCCATGGAATTTTGTTCTGGGAAAGGCAAGAATTGGCTTCTCACAAATATGAAGCTTGGAATAGAAGGGAGGATGCTCCCCCAAAGTCAGTTAGATCAGACAGTTCATCTCAAGGAAAAGGACAGGAAAGGCATCACGAGAGGGAGAACAATGTTCCTAAAAGAGACTTCCATGAAGCTTTACCTCGTGTACAGCCAAGTGTTGCTGGACCTCATGTGAAAAGCAACGGTCAAGGCTTATTTGCTGGTGACAATTATGGTGGCAAACAAAATGACGCAAAATTAGGAATTAAAGAAGAAGAGACGCCTAAGTTGAAGCCCCCCTACAATTATGGGGTCCCTCCTCCGTATGTGAAACCAAATGCCAAAGCAAAAGATAGAAAACATGAAGTCAAGGATGGTTCTGGCAGCAAGCGCATCACTAAGGATCCTGTAGCGTATAACATGGCCTTTGTTGACAATATACTCAGAGGGGTAAGCGTAGGATCTGATGATGAGGATGAGAAGCCTCGTGCTCATCGGGATGATGCATCTAGCAACCACATACCAAAACCAAGATCTTCTAGGAGGAGACACTCAAGATCACGGTCCGCTCACAATGATGCTGGAAACAGTGAAGATACAGAATTTGCTATCAGAAAAACAAGAAGCAGGAGAAAGGATGAGTCACGAGGAGGTCTGCAACTGTTGTTTGATGACGAGCACTATAAGAAGGATGAAGAAGAGAGGAGAATGGACGAGCTTCTGATTCATTACAGTAAAAAGCCATCTAACTTGGACTCAGAATTGTCAAGAAGAAAGTCGAGAAGTCGTCACGCACAGCATGCAGGCACTGGTGTTGGTGAATCCCCAAGACACGCAAGCAGAGATGAGTCGGAAATGGTTCCAACAGCAGTAAGATCAGTTTCCCTTCCTCATGAACATAGCGGTCCATCAGAGGCACCCAAAGTATTTGCTCGTGCGGCTTCGTTTCAACCCGACAGGTCCAATCCAGCTAAGCATGTGCATCCCAAGCTCCCAGACTACGAAGATTTGGCCGCCCAATTTGCAGCCCTAAGAGGAATGTTGAGTGGCACAGTGGCTTCCGACGTGGTGTTCATCATAAATAACAGTTGTAAATTTCAGTGTCAGTGCCAAATGACTAATTTGACCTTGATTGTCAGAAGGAAAAGCCTTTACATTCCCCAGGACCATTTGCCCTTCCACATTCCCCAGGACCATTTGCCCCTCTACGTTCCCTTCCCTTGTCATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

695

Amino Acids

78.83

Weight (kDa)

9.28

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 170 1.1e-39 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1768
AccBSI CCGCTC 1 cut(s) 1421
AccI GTMKAC 1 cut(s) 623
AccII CGCG 1 cut(s) 392
AciI CCGC 5 cut(s) 191, 1419, 1755, 1790, 1866
AclWI GGATC 4 cut(s) 80, 1250, 1263, 1321
AcoI YGGCCR 2 cut(s) 829, 1863
AcsI RAATTY 4 cut(s) 394, 837, 1454, 1946
AcuI CTGAAG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 1133
AfaI GTAC 2 cut(s) 724, 1020
AfiI CCNNNNNNNGG 1 cut(s) 672
AgsI TTSAA 7 cut(s) 380, 444, 481, 530, 733, 1144, 1802
AjiI CACGTC 1 cut(s) 1918
AjnI CCWGG 3 cut(s) 800, 2013, 2040
AluBI AGCT 7 cut(s) 440, 718, 884, 1007, 1570, 1823, 1843
AluI AGCT 7 cut(s) 440, 718, 884, 1007, 1570, 1823, 1843
Alw21I GWGCWC 3 cut(s) 249, 1345, 1533
Alw26I GTCTC 4 cut(s) 795, 987, 1124, 1393
AlwI GGATC 4 cut(s) 80, 1250, 1263, 1321
AlwNI CAGNNNCTG 1 cut(s) 1661
AoxI GGCC 9 cut(s) 19, 148, 446, 522, 572, 587, 829, 1275, 1863
ApeKI GCWGC 5 cut(s) 13, 326, 329, 1236, 1877
ApoI RAATTY 4 cut(s) 394, 837, 1454, 1946
Asp700I GAANNNNTTC 1 cut(s) 1710
AspLEI GCGC 1 cut(s) 1245
AspS9I GGNCC 9 cut(s) 19, 587, 1037, 1165, 1416, 1757, 1812, 2017, 2044
AsuHPI GGTGA 3 cut(s) 133, 1088, 1682
AvaII GGWCC 7 cut(s) 1037, 1165, 1416, 1757, 1812, 2017, 2044
AxyI CCTNAGG 2 cut(s) 288, 365
BalI TGGCCA 1 cut(s) 831
BamHI GGATCC 1 cut(s) 1255
BanI GGYRCC 1 cut(s) 1768
BarI GAAGNNNNNNTAC 2 cut(s) 1136, 1168
BauI CACGAG 5 cut(s) 965, 1014, 1338, 1497, 1785
BbsI GAAGAC 1 cut(s) 71
Bbv12I GWGCWC 3 cut(s) 249, 1345, 1533
BbvI GCAGC 4 cut(s) 313, 316, 1248, 1889
BccI CCATC 6 cut(s) 173, 532, 821, 1220, 1603, 1768
BceAI ACGGC 2 cut(s) 6, 204
BciT130I CCWGG 3 cut(s) 802, 2015, 2042
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 4 cut(s) 795, 987, 1124, 1393
BfaI CTAG 2 cut(s) 1362, 1392
BfmI CTRYAG 2 cut(s) 636, 1260
BglII AGATCT 1 cut(s) 1385
BisI GCNGC 7 cut(s) 14, 327, 330, 1237, 1791, 1866, 1878
BlpI GCTNAGC 1 cut(s) 1824
BlsI GCNGC 7 cut(s) 15, 328, 331, 1238, 1792, 1867, 1879
Bme1390I CCNGG 3 cut(s) 802, 2015, 2042
Bme18I GGWCC 7 cut(s) 1037, 1165, 1416, 1757, 1812, 2017, 2044
BmgBI CACGTC 1 cut(s) 1918
BmgT120I GGNCC 9 cut(s) 19, 587, 1037, 1165, 1416, 1757, 1812, 2017, 2044
BmiI GGNNCC 6 cut(s) 303, 1166, 1167, 1257, 1713, 1770
BmrFI CCNGG 3 cut(s) 802, 2015, 2042
BmrI ACTGGG 1 cut(s) 636
BmsI GCATC 9 cut(s) 221, 682, 892, 970, 1254, 1345, 1367, 1420, 1843
BmuI ACTGGG 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 422, 479
Bpu1102I GCTNAGC 1 cut(s) 1824
BpuEI CTTGAG 3 cut(s) 233, 924, 1390
Bsa29I ATCGAT 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 1133
BsaJI CCNNGG 6 cut(s) 22, 519, 801, 832, 2013, 2040
Bsc4I CCNNNNNNNGG 1 cut(s) 672
Bse1I ACTGG 3 cut(s) 462, 631, 1666
Bse21I CCTNAGG 2 cut(s) 288, 365
BseBI CCWGG 3 cut(s) 802, 2015, 2042
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 6 cut(s) 22, 519, 801, 832, 2013, 2040
BseGI GGATG 8 cut(s) 543, 907, 1231, 1333, 1357, 1495, 1549, 1834
BseLI CCNNNNNNNGG 1 cut(s) 672
BseMII CTCAG 5 cut(s) 279, 710, 808, 1309, 1623
BseNI ACTGG 3 cut(s) 462, 631, 1666
BseRI GAGGAG 5 cut(s) 540, 1161, 1411, 1515, 1570
BseXI GCAGC 4 cut(s) 313, 316, 1248, 1889
BseYI CCCAGC 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 392
BshFI GGCC 9 cut(s) 21, 150, 448, 524, 574, 589, 831, 1277, 1865
BshNI GGYRCC 1 cut(s) 1768
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 3 cut(s) 249, 1345, 1533
BslFI GGGAC 3 cut(s) 527, 626, 1151
BslI CCNNNNNNNGG 1 cut(s) 672
BsmAI GTCTC 4 cut(s) 795, 987, 1124, 1393
BsmBI CGTCTC 1 cut(s) 1124
BsmFI GGGAC 3 cut(s) 527, 626, 1151
BsnI GGCC 9 cut(s) 21, 150, 448, 524, 574, 589, 831, 1277, 1865
Bsp1286I GDGCHC 3 cut(s) 249, 1345, 1533
Bsp1407I TGTACA 1 cut(s) 1018
Bsp1720I GCTNAGC 1 cut(s) 1824
Bsp19I CCATGG 1 cut(s) 832
BspACI CCGC 5 cut(s) 191, 1419, 1755, 1790, 1866
BspANI GGCC 9 cut(s) 21, 150, 448, 524, 574, 589, 831, 1277, 1865
BspCNI CTCAG 5 cut(s) 280, 709, 807, 1308, 1622
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 1 cut(s) 392
BspHI TCATGA 1 cut(s) 1744
BspLI GGNNCC 6 cut(s) 303, 1166, 1167, 1257, 1713, 1770
BspMAI CTGCAG 1 cut(s) 640
BspPI GGATC 4 cut(s) 80, 1250, 1263, 1321
BspT107I GGYRCC 1 cut(s) 1768
BsrBI CCGCTC 1 cut(s) 1421
BsrGI TGTACA 1 cut(s) 1018
BsrI ACTGG 3 cut(s) 462, 631, 1666
BssECI CCNNGG 6 cut(s) 22, 519, 801, 832, 2013, 2040
BssNI GRCGYC 1 cut(s) 1133
BssSI CACGAG 5 cut(s) 965, 1014, 1338, 1497, 1785
BssT1I CCWWGG 2 cut(s) 519, 832
Bst2BI CACGAG 5 cut(s) 965, 1014, 1338, 1497, 1785
Bst2UI CCWGG 3 cut(s) 802, 2015, 2042
Bst4CI ACNGT 9 cut(s) 814, 932, 1058, 1416, 1442, 1515, 1586, 1906, 1940
Bst6I CTCTTC 3 cut(s) 86, 1122, 1545
BstACI GRCGYC 1 cut(s) 1133
BstAPI GCANNNNNTGC 1 cut(s) 1652
BstAUI TGTACA 1 cut(s) 1018
BstC8I GCNNGC 4 cut(s) 1241, 1653, 1657, 1690
BstDSI CCRYGG 2 cut(s) 22, 832
BstF5I GGATG 8 cut(s) 543, 907, 1231, 1333, 1357, 1495, 1549, 1834
BstFNI CGCG 1 cut(s) 392
BstHHI GCGC 1 cut(s) 1245
BstMAI GTCTC 4 cut(s) 795, 987, 1124, 1393
BstMWI GCNNNNNNNGC 5 cut(s) 1242, 1652, 1790, 1840, 1874
BstNI CCWGG 3 cut(s) 802, 2015, 2042
BstNSI RCATGY 2 cut(s) 1655, 1832
BstSCI CCNGG 3 cut(s) 800, 2013, 2040
BstSFI CTRYAG 2 cut(s) 636, 1260
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 4 cut(s) 313, 316, 1248, 1889
BstV2I GAAGAC 1 cut(s) 71
BstX2I RGATCY 3 cut(s) 1255, 1313, 1385
BstYI RGATCY 3 cut(s) 1255, 1313, 1385
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 2 cut(s) 288, 365
BsuRI GGCC 9 cut(s) 21, 150, 448, 524, 574, 589, 831, 1277, 1865
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 2 cut(s) 22, 832
BtgZI GCGATG 2 cut(s) 244, 297
BtrI CACGTC 1 cut(s) 1918
BtsCI GGATG 8 cut(s) 543, 907, 1231, 1333, 1357, 1495, 1549, 1834
BtsIMutI CAGTG 7 cut(s) 455, 802, 1447, 1659, 1911, 1958, 1964
Cac8I GCNNGC 4 cut(s) 1241, 1653, 1657, 1690
CaiI CAGNNNCTG 1 cut(s) 1661
CciI TCATGA 1 cut(s) 1744
CfoI GCGC 1 cut(s) 1245
Cfr13I GGNCC 9 cut(s) 19, 587, 1037, 1165, 1416, 1757, 1812, 2017, 2044
ClaI ATCGAT 1 cut(s) 75
CpoI CGGWCCG 1 cut(s) 1416
CseI GACGC 2 cut(s) 1112, 1141
Csp6I GTAC 2 cut(s) 723, 1019
CspCI CAANNNNNGTGG 2 cut(s) 1069, 1104
CspI CGGWCCG 1 cut(s) 1416
CviAII CATG 9 cut(s) 817, 833, 1001, 1043, 1214, 1273, 1652, 1745, 1829
CviQI GTAC 2 cut(s) 723, 1019
EaeI YGGCCR 2 cut(s) 829, 1863
Eam1104I CTCTTC 3 cut(s) 86, 1122, 1545
EarI CTCTTC 3 cut(s) 86, 1122, 1545
EciI GGCGGA 1 cut(s) 206
Eco130I CCWWGG 2 cut(s) 519, 832
Eco147I AGGCCT 2 cut(s) 448, 524
Eco32I GATATC 2 cut(s) 127, 157
Eco47I GGWCC 7 cut(s) 1037, 1165, 1416, 1757, 1812, 2017, 2044
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 2 cut(s) 288, 365
EcoO109I RGGNCCY 1 cut(s) 1165
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 3 cut(s) 800, 2013, 2040
EcoRV GATATC 2 cut(s) 127, 157
EcoT14I CCWWGG 2 cut(s) 519, 832
EcoT22I ATGCAT 1 cut(s) 1360
ErhI CCWWGG 2 cut(s) 519, 832
Esp3I CGTCTC 1 cut(s) 1124
FaeI CATG 9 cut(s) 820, 836, 1004, 1046, 1217, 1276, 1655, 1748, 1832
FaqI GGGAC 3 cut(s) 527, 626, 1151
FatI CATG 9 cut(s) 816, 832, 1000, 1042, 1213, 1272, 1651, 1744, 1828
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 7 cut(s) 14, 327, 330, 1237, 1791, 1866, 1878
FokI GGATG 8 cut(s) 550, 914, 1238, 1340, 1364, 1502, 1556, 1821
Fsp4HI GCNGC 7 cut(s) 14, 327, 330, 1237, 1791, 1866, 1878
FspBI CTAG 2 cut(s) 1362, 1392
GlaI GCGC 1 cut(s) 1244
GluI GCNGC 7 cut(s) 14, 327, 330, 1237, 1791, 1866, 1878
GsaI CCCAGC 1 cut(s) 20
GsuI CTGGAG 2 cut(s) 422, 479
HaeIII GGCC 9 cut(s) 21, 150, 448, 524, 574, 589, 831, 1277, 1865
HgaI GACGC 2 cut(s) 1112, 1141
HhaI GCGC 1 cut(s) 1245
Hin1I GRCGYC 1 cut(s) 1133
Hin1II CATG 9 cut(s) 820, 836, 1004, 1046, 1217, 1276, 1655, 1748, 1832
Hin6I GCGC 1 cut(s) 1243
HinP1I GCGC 1 cut(s) 1243
HincII GTYRAC 1 cut(s) 1285
HindII GTYRAC 1 cut(s) 1285
HindIII AAGCTT 2 cut(s) 882, 1005
HphI GGTGA 3 cut(s) 133, 1088, 1682
Hpy166II GTNNAC 4 cut(s) 561, 624, 1019, 1285
Hpy8I GTNNAC 4 cut(s) 561, 624, 1019, 1285
Hpy99I CGWCG 2 cut(s) 64, 1919
HpyCH4III ACNGT 9 cut(s) 814, 932, 1058, 1416, 1442, 1515, 1586, 1906, 1940
HpyCH4IV ACGT 3 cut(s) 747, 1917, 2061
HpyCH4V TGCA 9 cut(s) 42, 268, 332, 638, 1358, 1510, 1655, 1834, 1877
HpyF10VI GCNNNNNNNGC 5 cut(s) 1242, 1652, 1790, 1840, 1874
HpySE526I ACGT 3 cut(s) 747, 1917, 2061
Hsp92I GRCGYC 1 cut(s) 1133
Hsp92II CATG 9 cut(s) 820, 836, 1004, 1046, 1217, 1276, 1655, 1748, 1832
HspAI GCGC 1 cut(s) 1243
KflI GGGWCCC 1 cut(s) 1165
Ksp22I TGATCA 1 cut(s) 742
LmnI GCTCC 2 cut(s) 910, 1848
Lsp1109I GCAGC 4 cut(s) 313, 316, 1248, 1889
LweI GCATC 9 cut(s) 221, 682, 892, 970, 1254, 1345, 1367, 1420, 1843
MaeI CTAG 2 cut(s) 1362, 1392
MaeII ACGT 3 cut(s) 747, 1917, 2061
MaeIII GTNAC 4 cut(s) 359, 1076, 1494, 1640
MbiI CCGCTC 1 cut(s) 1421
MflI RGATCY 3 cut(s) 1255, 1313, 1385
MhlI GDGCHC 3 cut(s) 249, 1345, 1533
MlsI TGGCCA 1 cut(s) 831
MluNI TGGCCA 1 cut(s) 831
MlyI GAGTC 6 cut(s) 61, 177, 509, 1502, 1601, 1709
MmeI TCCRAC 3 cut(s) 1683, 1739, 1938
Mox20I TGGCCA 1 cut(s) 831
Mph1103I ATGCAT 1 cut(s) 1360
MroXI GAANNNNTTC 1 cut(s) 1710
MscI TGGCCA 1 cut(s) 831
MseI TTAA 4 cut(s) 54, 197, 687, 1119
MslI CAYNNNNRTG 4 cut(s) 600, 876, 1350, 1428
Msp20I TGGCCA 1 cut(s) 831
MspR9I CCNGG 3 cut(s) 802, 2015, 2042
MvaI CCWGG 3 cut(s) 802, 2015, 2042
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 5 cut(s) 1242, 1652, 1790, 1840, 1874
NcoI CCATGG 1 cut(s) 832
NlaIII CATG 9 cut(s) 820, 836, 1004, 1046, 1217, 1276, 1655, 1748, 1832
NlaIV GGNNCC 6 cut(s) 303, 1166, 1167, 1257, 1713, 1770
NmuCI GTSAC 4 cut(s) 359, 1076, 1494, 1640
NsiI ATGCAT 1 cut(s) 1360
NspI RCATGY 2 cut(s) 1655, 1832
PaeI GCATGC 1 cut(s) 1655
PagI TCATGA 1 cut(s) 1744
PceI AGGCCT 2 cut(s) 448, 524
PdmI GAANNNNTTC 1 cut(s) 1710
PfeI GAWTC 4 cut(s) 253, 415, 1576, 1673
PkrI GCNGC 7 cut(s) 15, 328, 331, 1238, 1792, 1867, 1879
PleI GAGTC 6 cut(s) 61, 177, 509, 1501, 1601, 1708
PpsI GAGTC 6 cut(s) 61, 177, 509, 1501, 1601, 1708
PpuMI RGGWCCY 1 cut(s) 1165
Psp5II RGGWCCY 1 cut(s) 1165
Psp6I CCWGG 3 cut(s) 800, 2013, 2040
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 3 cut(s) 800, 2013, 2040
PspN4I GGNNCC 6 cut(s) 303, 1166, 1167, 1257, 1713, 1770
PspPI GGNCC 9 cut(s) 19, 587, 1037, 1165, 1416, 1757, 1812, 2017, 2044
PspPPI RGGWCCY 1 cut(s) 1165
PstI CTGCAG 1 cut(s) 640
PstNI CAGNNNCTG 1 cut(s) 1661
PsuI RGATCY 3 cut(s) 1255, 1313, 1385
RsaI GTAC 2 cut(s) 724, 1020
RsaNI GTAC 2 cut(s) 723, 1019
RseI CAYNNNNRTG 4 cut(s) 600, 876, 1350, 1428
Rsr2I CGGWCCG 1 cut(s) 1416
RsrII CGGWCCG 1 cut(s) 1416
SaqAI TTAA 4 cut(s) 54, 197, 687, 1119
SatI GCNGC 7 cut(s) 14, 327, 330, 1237, 1791, 1866, 1878
Sau96I GGNCC 9 cut(s) 19, 587, 1037, 1165, 1416, 1757, 1812, 2017, 2044
SchI GAGTC 6 cut(s) 61, 177, 509, 1502, 1601, 1709
ScrFI CCNGG 3 cut(s) 802, 2015, 2042
SduI GDGCHC 3 cut(s) 249, 1345, 1533
SfaNI GCATC 9 cut(s) 221, 682, 892, 970, 1254, 1345, 1367, 1420, 1843
SfcI CTRYAG 2 cut(s) 636, 1260
SinI GGWCC 7 cut(s) 1037, 1165, 1416, 1757, 1812, 2017, 2044
SmiMI CAYNNNNRTG 4 cut(s) 600, 876, 1350, 1428
SmlI CTYRAG 3 cut(s) 248, 939, 1405
SmoI CTYRAG 3 cut(s) 248, 939, 1405
SphI GCATGC 1 cut(s) 1655
SseBI AGGCCT 2 cut(s) 448, 524
SsiI CCGC 5 cut(s) 191, 1419, 1755, 1790, 1866
SspMI CTAG 2 cut(s) 1362, 1392
StuI AGGCCT 2 cut(s) 448, 524
StyD4I CCNGG 3 cut(s) 800, 2013, 2040
StyI CCWWGG 2 cut(s) 519, 832
TaaI ACNGT 9 cut(s) 814, 932, 1058, 1416, 1442, 1515, 1586, 1906, 1940
TaiI ACGT 3 cut(s) 750, 1920, 2064
TaqI TCGA 3 cut(s) 59, 75, 1631
TatI WGTACW 1 cut(s) 1018
TauI GCSGC 2 cut(s) 1793, 1868
TfiI GAWTC 4 cut(s) 253, 415, 1576, 1673
Tru1I TTAA 4 cut(s) 54, 197, 687, 1119
Tru9I TTAA 4 cut(s) 54, 197, 687, 1119
TscAI CASTG 7 cut(s) 462, 802, 1447, 1666, 1911, 1958, 1964
TseFI GTSAC 4 cut(s) 359, 1076, 1494, 1640
TseI GCWGC 5 cut(s) 13, 326, 329, 1236, 1877
Tsp45I GTSAC 4 cut(s) 359, 1076, 1494, 1640
TspDTI ATGAA 8 cut(s) 894, 924, 1017, 1230, 1560, 1568, 1761, 1915
TspGWI ACGGA 2 cut(s) 100, 1164
TspRI CASTG 7 cut(s) 462, 802, 1447, 1666, 1911, 1958, 1964
VpaK11BI GGWCC 7 cut(s) 1037, 1165, 1416, 1757, 1812, 2017, 2044
XapI RAATTY 4 cut(s) 394, 837, 1454, 1946
XceI RCATGY 2 cut(s) 1655, 1832
XmiI GTMKAC 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 1710
XspI CTAG 2 cut(s) 1362, 1392
Zsp2I ATGCAT 1 cut(s) 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.