pycom15g17280

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011073
Physical Location & Seq
Reverse (-)
12176142 .. 12177413
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1272 bp
ATGATTAGATTTTTGGGTCAGTCTCAAAATTTCAAATTTCCAATGTTCCATTCAAGTTTGATTGTTTTTTTGCTTCTGAATTTTGTGGTGTTCCATGTTGAAGCACAATCCAGTGGCCCAGATTATCCTTCCGATGAACAAAGCAATTTTCAACCAAGTGTTGCTGTGGTTATTGGAATTCTAGCAGTCATGTTTGCACTGACTTTTGTCCTCCTTGTGTATGCAAAACTCTGCCACAGGAGAGCAGGTGTGGTTGGAAACAACAATCTCCAGCCAACAGGACTTATCAGTAGAAGCTCTCGCTTTTCGGGGATTGACAAGACAGTGATCGAGGCACTTCCTTTTTTCAGATTCTCTTCACTGAAAGGGTCGAAGGAGGGCCTCGAATGCTCAGTCTGCCTGTCGAAATTCGAAGGTGTTGAAATCCTCAGGCTGCTTCCCAAATGCAAGCATGCTTTTCACATCGGCTGCATTGATCACTGGCTCGAAAAGCACTCGAGCTGTCCTCTCTGCAGGCACAGGGTTAGTGCTGAGGAGTTGACTTCCATTACCTACTCAAACAGCATGAGGTGGATCGATCAATCAGAGCGCCGCCAGGACTCGAACATAGAGCTCTTTGTTCAGAGAGAGGAGGATCACCGCAGTTCTTCAAGATTCAGCGTAGGAAGCAGCTTTCGGAAAGTTGACAAGGCGATTGATAAGGAACTGTTGATCCAAGAAGCTGCTGATGTGACAGAGGAAGATCAGAAAGCCCTGCACAAGCACAAGCACACAATTGTTGTTTCGGATCTTGAGTTTAAGAACAGATGGAGCAATTTGAGTTCCTCTGACCTCATGTTCTTGAATTCCGAGATGCTTAACACGAAGTCAAGCAATAGATTTTCTTTCAGACATTTGAATGATGAGCAGTTTTCGCCGGTGAAAGCAACAGGGAATGAGGAGATTGTAAAGATTAAGGAGGACAAAGTTGGTACAATGAACAAAAATAGCTCAGCTTCGGTTCCTTCTGATTCAGACAGCATAGCAGCTTCAGCTCACGCCTCGAGTTCAGTGAAACGAGGAGAGAAAAGATCCATGTCCGAAATCACTGGTCTTTCGAGATTTGGGAATTTGGGCTTTAAGAACAGGACTAGAGAGCCTTCTCTGCTCGAAAACAATGCGGAGGAGGAAAGAAGGAGGCGGCTTTGGTTGCCGATTGCCAGTAGAACAGTCCAGTGGTTTGCCAATAGAGAGAATAGGCCTCCACAGCCTCAACAGCCTTTAGATGTATAA

Protein Analysis

424

Amino Acids

48.03

Weight (kDa)

8.88

Isoelectric Point (pI)

66.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 129 - 172 1.3e-12 Ring finger domain
zf-RING_11 PF17123 129 - 158 5e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 129 - 171 3e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 130 - 171 1.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 136 - 176 5.9e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-rbx1 PF12678 142 - 172 6.2e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20030 AT4G28890
fragaria_vesca FvH4_1g05750
malus_domestica MD02G1058600.v1.1 MD02G1058700.v1.1 MD15G1192900.v1.1 MD15G1193000.v1.1
prunus_persica Prupe.7G224300_v2.0.a1
pyrus_communis pycom02g04750 pycom15g17270 pycom15g17280
rosa_chinensis RchiOBHm_Chr2g0091391
rosa_laevigata RLG00000016220
rosa_multiflora Rmu_ssc0000333.1_g000003
rosa_rugosa Rorug02G0017400
rosa_samantha Rh2AG062400 Rh2BG061400 Rh2CG063500 Rh2DG062000
rosa_wichuraiana Rw2G005780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
Acc36I ACCTGC 1 cut(s) 236
AciI CCGC 4 cut(s) 592, 640, 1160, 1180
AclWI GGATC 5 cut(s) 581, 642, 706, 795, 1065
AcsI RAATTY 7 cut(s) 28, 35, 79, 177, 407, 844, 1108
AcuI CTGAAG 1 cut(s) 1014
AfaI GTAC 1 cut(s) 973
AgsI TTSAA 8 cut(s) 34, 54, 101, 152, 422, 651, 844, 898
AjnI CCWGG 1 cut(s) 594
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AloI GAACNNNNNNTCC 2 cut(s) 696, 728
AluBI AGCT 9 cut(s) 297, 501, 613, 672, 722, 990, 995, 1028, 1034
AluI AGCT 9 cut(s) 297, 501, 613, 672, 722, 990, 995, 1028, 1034
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 5 cut(s) 581, 642, 706, 795, 1065
Ama87I CYCGRG 2 cut(s) 496, 1042
AoxI GGCC 3 cut(s) 115, 379, 1238
ApeKI GCWGC 5 cut(s) 433, 468, 669, 722, 1025
ApoI RAATTY 7 cut(s) 28, 35, 79, 177, 407, 844, 1108
ArsI GACNNNNNNTTYG 2 cut(s) 378, 410
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 2 cut(s) 116, 379
AsuHPI GGTGA 2 cut(s) 629, 931
AsuII TTCGAA 1 cut(s) 411
AvaI CYCGRG 2 cut(s) 496, 1042
AxyI CCTNAGG 1 cut(s) 428
BanII GRGCYC 1 cut(s) 615
Bbv12I GWGCWC 1 cut(s) 615
BbvCI CCTCAGC 1 cut(s) 531
BbvI GCAGC 5 cut(s) 420, 455, 681, 709, 1037
BccI CCATC 1 cut(s) 801
BcgI CGANNNNNNTGC 2 cut(s) 1139, 1173
BciT130I CCWGG 1 cut(s) 596
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 2 cut(s) 182, 1131
BfmI CTRYAG 1 cut(s) 511
BfoI RGCGCY 1 cut(s) 592
BfuAI ACCTGC 1 cut(s) 236
BisI GCNGC 7 cut(s) 434, 469, 592, 670, 723, 1026, 1181
BlpI GCTNAGC 1 cut(s) 991
BlsI GCNGC 7 cut(s) 435, 470, 593, 671, 724, 1027, 1182
Bme1390I CCNGG 1 cut(s) 596
BmeT110I CYCGRG 2 cut(s) 496, 1042
BmgT120I GGNCC 2 cut(s) 116, 379
BmiI GGNNCC 1 cut(s) 1002
BmrFI CCNGG 1 cut(s) 596
BmsI GCATC 1 cut(s) 843
BoxI GACNNNNGTC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 490, 522
BpmI CTGGAG 1 cut(s) 254
Bpu10I CCTNAGC 1 cut(s) 531
Bpu1102I GCTNAGC 1 cut(s) 991
Bpu14I TTCGAA 1 cut(s) 411
BpuEI CTTGAG 1 cut(s) 812
Bsa29I ATCGAT 1 cut(s) 576
BsaXI ACNNNNNCTCC 2 cut(s) 232, 262
Bse118I RCCGGY 1 cut(s) 916
Bse1I ACTGG 5 cut(s) 111, 485, 1093, 1200, 1213
Bse21I CCTNAGG 1 cut(s) 428
BseBI CCWGG 1 cut(s) 596
BseCI ATCGAT 1 cut(s) 576
BseMII CTCAG 4 cut(s) 405, 442, 522, 1005
BseNI ACTGG 5 cut(s) 111, 485, 1093, 1200, 1213
BseRI GAGGAG 5 cut(s) 548, 644, 953, 1074, 1178
BseXI GCAGC 5 cut(s) 420, 455, 681, 709, 1037
BsgI GTGCAG 1 cut(s) 740
BshFI GGCC 3 cut(s) 117, 381, 1240
BshVI ATCGAT 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 615
BsiHKCI CYCGRG 2 cut(s) 496, 1042
BsiSI CCGG 1 cut(s) 917
BsmAI GTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 3 cut(s) 117, 381, 1240
BsoBI CYCGRG 2 cut(s) 496, 1042
Bsp119I TTCGAA 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 615
Bsp143I GATC 9 cut(s) 327, 475, 573, 577, 634, 711, 742, 787, 1070
Bsp1720I GCTNAGC 1 cut(s) 991
BspACI CCGC 4 cut(s) 592, 640, 1160, 1180
BspANI GGCC 3 cut(s) 117, 381, 1240
BspCNI CTCAG 4 cut(s) 404, 441, 523, 1004
BspDI ATCGAT 1 cut(s) 576
BspLI GGNNCC 1 cut(s) 1002
BspMAI CTGCAG 1 cut(s) 515
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 5 cut(s) 581, 642, 706, 795, 1065
BspT104I TTCGAA 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 916
BsrI ACTGG 5 cut(s) 111, 485, 1093, 1200, 1213
BssAI RCCGGY 1 cut(s) 916
BssMI GATC 9 cut(s) 327, 475, 573, 577, 634, 711, 742, 787, 1070
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 3 cut(s) 325, 708, 1210
Bst6I CTCTTC 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 411
BstC8I GCNNGC 3 cut(s) 449, 453, 515
BstDEI CTNAG 4 cut(s) 391, 428, 531, 991
BstH2I RGCGCY 1 cut(s) 592
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 9 cut(s) 330, 478, 576, 580, 637, 714, 745, 790, 1073
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 9 cut(s) 327, 475, 573, 577, 634, 711, 742, 787, 1070
BstNI CCWGG 1 cut(s) 596
BstNSI RCATGY 1 cut(s) 455
BstPAI GACNNNNGTC 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 594
BstSFI CTRYAG 1 cut(s) 511
BstV1I GCAGC 5 cut(s) 420, 455, 681, 709, 1037
BstX2I RGATCY 2 cut(s) 787, 1070
BstYI RGATCY 2 cut(s) 787, 1070
Bsu15I ATCGAT 1 cut(s) 576
Bsu36I CCTNAGG 1 cut(s) 428
BsuRI GGCC 3 cut(s) 117, 381, 1240
BsuTUI ATCGAT 1 cut(s) 576
BtsIMutI CAGTG 8 cut(s) 118, 197, 330, 359, 478, 1056, 1086, 1220
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 3 cut(s) 449, 453, 515
CfoI GCGC 1 cut(s) 591
Cfr10I RCCGGY 1 cut(s) 916
Cfr13I GGNCC 2 cut(s) 116, 379
ClaI ATCGAT 1 cut(s) 576
Csp6I GTAC 1 cut(s) 972
CviAII CATG 6 cut(s) 95, 190, 452, 565, 835, 1075
CviQI GTAC 1 cut(s) 972
DdeI CTNAG 4 cut(s) 391, 428, 531, 991
DpnI GATC 9 cut(s) 329, 477, 575, 579, 636, 713, 744, 789, 1072
DpnII GATC 9 cut(s) 327, 475, 573, 577, 634, 711, 742, 787, 1070
Eam1104I CTCTTC 1 cut(s) 361
EarI CTCTTC 1 cut(s) 361
Ecl136II GAGCTC 1 cut(s) 613
Eco147I AGGCCT 1 cut(s) 1240
Eco24I GRGCYC 1 cut(s) 615
Eco53kI GAGCTC 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 1014
Eco81I CCTNAGG 1 cut(s) 428
Eco88I CYCGRG 2 cut(s) 496, 1042
EcoICRI GAGCTC 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 379
EcoRI GAATTC 2 cut(s) 177, 844
EcoRII CCWGG 1 cut(s) 594
EcoT38I GRGCYC 1 cut(s) 615
FaeI CATG 6 cut(s) 98, 193, 455, 568, 838, 1078
FatI CATG 6 cut(s) 94, 189, 451, 564, 834, 1074
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 7 cut(s) 434, 469, 592, 670, 723, 1026, 1181
FriOI GRGCYC 1 cut(s) 615
Fsp4HI GCNGC 7 cut(s) 434, 469, 592, 670, 723, 1026, 1181
FspBI CTAG 2 cut(s) 182, 1131
GlaI GCGC 1 cut(s) 590
GluI GCNGC 7 cut(s) 434, 469, 592, 670, 723, 1026, 1181
GsuI CTGGAG 1 cut(s) 254
HaeII RGCGCY 1 cut(s) 592
HaeIII GGCC 3 cut(s) 117, 381, 1240
HapII CCGG 1 cut(s) 917
HhaI GCGC 1 cut(s) 591
Hin1II CATG 6 cut(s) 98, 193, 455, 568, 838, 1078
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HincII GTYRAC 2 cut(s) 540, 685
HindII GTYRAC 2 cut(s) 540, 685
HinfI GANTC 4 cut(s) 351, 599, 654, 1010
HpaII CCGG 1 cut(s) 917
HphI GGTGA 2 cut(s) 629, 931
Hpy166II GTNNAC 2 cut(s) 540, 685
Hpy188III TCNNGA 4 cut(s) 651, 791, 841, 1098
Hpy8I GTNNAC 2 cut(s) 540, 685
HpyAV CCTTC 6 cut(s) 138, 367, 407, 1014, 1149, 1167
HpyCH4III ACNGT 3 cut(s) 325, 708, 1210
HpyCH4V TGCA 6 cut(s) 197, 224, 447, 471, 513, 757
HpyF3I CTNAG 4 cut(s) 391, 428, 531, 991
Hsp92II CATG 6 cut(s) 98, 193, 455, 568, 838, 1078
HspAI GCGC 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 9 cut(s) 327, 475, 573, 577, 634, 711, 742, 787, 1070
LmnI GCTCC 1 cut(s) 810
Lsp1109I GCAGC 5 cut(s) 420, 455, 681, 709, 1037
LweI GCATC 1 cut(s) 843
MaeI CTAG 2 cut(s) 182, 1131
MaeIII GTNAC 1 cut(s) 730
MalI GATC 9 cut(s) 329, 477, 575, 579, 636, 713, 744, 789, 1072
MboI GATC 9 cut(s) 327, 475, 573, 577, 634, 711, 742, 787, 1070
MboII GAAGA 3 cut(s) 348, 639, 752
MfeI CAATTG 1 cut(s) 774
MflI RGATCY 2 cut(s) 787, 1070
MhlI GDGCHC 1 cut(s) 615
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 235
MseI TTAA 4 cut(s) 798, 858, 954, 1119
MslI CAYNNNNRTG 1 cut(s) 897
MspI CCGG 1 cut(s) 917
MspR9I CCNGG 1 cut(s) 596
MunI CAATTG 1 cut(s) 774
Mva1269I GAATGC 1 cut(s) 392
MvaI CCWGG 1 cut(s) 596
NdeII GATC 9 cut(s) 327, 475, 573, 577, 634, 711, 742, 787, 1070
NlaIII CATG 6 cut(s) 98, 193, 455, 568, 838, 1078
NlaIV GGNNCC 1 cut(s) 1002
NmuCI GTSAC 1 cut(s) 730
NspI RCATGY 1 cut(s) 455
NspV TTCGAA 1 cut(s) 411
PaeI GCATGC 1 cut(s) 455
PaeR7I CTCGAG 2 cut(s) 496, 1042
PaqCI CACCTGC 1 cut(s) 236
PceI AGGCCT 1 cut(s) 1240
PctI GAATGC 1 cut(s) 392
PfeI GAWTC 3 cut(s) 351, 654, 1010
PkrI GCNGC 7 cut(s) 435, 470, 593, 671, 724, 1027, 1182
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
PshAI GACNNNNGTC 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 594
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 1 cut(s) 1002
PspPI GGNCC 2 cut(s) 116, 379
PspXI VCTCGAGB 2 cut(s) 496, 1042
PstI CTGCAG 1 cut(s) 515
PsuI RGATCY 2 cut(s) 787, 1070
RsaI GTAC 1 cut(s) 973
RsaNI GTAC 1 cut(s) 972
RseI CAYNNNNRTG 1 cut(s) 897
SacI GAGCTC 1 cut(s) 615
SaqAI TTAA 4 cut(s) 798, 858, 954, 1119
SatI GCNGC 7 cut(s) 434, 469, 592, 670, 723, 1026, 1181
Sau3AI GATC 9 cut(s) 327, 475, 573, 577, 634, 711, 742, 787, 1070
Sau96I GGNCC 2 cut(s) 116, 379
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 1 cut(s) 596
SduI GDGCHC 1 cut(s) 615
SfaNI GCATC 1 cut(s) 843
SfcI CTRYAG 1 cut(s) 511
Sfr274I CTCGAG 2 cut(s) 496, 1042
SfuI TTCGAA 1 cut(s) 411
SgrAI CRCCGGYG 1 cut(s) 916
SlaI CTCGAG 2 cut(s) 496, 1042
SmiMI CAYNNNNRTG 1 cut(s) 897
SmlI CTYRAG 3 cut(s) 496, 791, 1042
SmoI CTYRAG 3 cut(s) 496, 791, 1042
SphI GCATGC 1 cut(s) 455
SseBI AGGCCT 1 cut(s) 1240
SsiI CCGC 4 cut(s) 592, 640, 1160, 1180
SspMI CTAG 2 cut(s) 182, 1131
SstI GAGCTC 1 cut(s) 615
StuI AGGCCT 1 cut(s) 1240
StyD4I CCNGG 1 cut(s) 594
TaaI ACNGT 3 cut(s) 325, 708, 1210
TauI GCSGC 2 cut(s) 594, 1183
TfiI GAWTC 3 cut(s) 351, 654, 1010
Tru1I TTAA 4 cut(s) 798, 858, 954, 1119
Tru9I TTAA 4 cut(s) 798, 858, 954, 1119
TscAI CASTG 8 cut(s) 118, 204, 330, 366, 485, 1056, 1093, 1220
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 5 cut(s) 433, 468, 669, 722, 1025
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 2 cut(s) 150, 992
TspRI CASTG 8 cut(s) 118, 204, 330, 366, 485, 1056, 1093, 1220
XapI RAATTY 7 cut(s) 28, 35, 79, 177, 407, 844, 1108
XceI RCATGY 1 cut(s) 455
XhoI CTCGAG 2 cut(s) 496, 1042
XspI CTAG 2 cut(s) 182, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.