pycom15g17950

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009948
Physical Location & Seq
Forward (+)
12748387 .. 12750495
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGAGGAAGCTGAGCAGAAAATTGAGAAATTCACAGGACGATGACTTCTCTCTACCCACTTGGGACGATCCTTCCCTCAACGATTCTCGCCCCATGGATACTCAAGAGCAGGAGGAGTTGGTTCGTGAAATGGAGAAGAGTCAAGCTCAACAGAGTCGGTTATGGAGGGCTTTGTTTGCATTGCTTCTCTTTTGTTTCGCGGTGTTTCTGTTGTACTCAATCTGTCAGCAGGTTCTGTCTCCATGGGAATTGCGTTATCATGCTTATTTCATGGAAGAGATGCTCTCATGGATGATTATATCTGCAGATTGGCTAGCTGTTGTAACATATTCATCAGCTATCATAGGATTGCTTCATGATTCAAAGCATCACCGCCAATGGATTTGGTACTCACTCTTTAGTGGCATTGTACTTGCAGCTTTCTGGCTGTATTACATGTTGAGATTGCCAAGGTTCCGCTGGGATGTAATCTGGCTTCCATTTGGACCTCTAAGTGGAGCAGCACTGGCTCTGTACGTTGATCGATCTGCTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

21.2

Weight (kDa)

6.06

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 220
AccII CGCG 1 cut(s) 200
AciI CCGC 3 cut(s) 200, 373, 457
AclWI GGATC 1 cut(s) 62
AcsI RAATTY 1 cut(s) 28
AfaI GTAC 4 cut(s) 215, 389, 411, 515
AfiI CCNNNNNNNGG 1 cut(s) 494
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 1 cut(s) 363
AluBI AGCT 5 cut(s) 10, 146, 317, 338, 419
AluI AGCT 5 cut(s) 10, 146, 317, 338, 419
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 1 cut(s) 235
ApeKI GCWGC 2 cut(s) 416, 500
ApoI RAATTY 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 362
AsuNHI GCTAGC 1 cut(s) 313
AvaII GGWCC 1 cut(s) 485
BarI GAAGNNNNNNTAC 2 cut(s) 459, 491
BbvI GCAGC 2 cut(s) 428, 512
BciVI GTATCC 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 1 cut(s) 314
BfmI CTRYAG 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 220
BfuI GTATCC 1 cut(s) 91
BisI GCNGC 2 cut(s) 417, 501
BlpI GCTNAGC 1 cut(s) 11
BlsI GCNGC 2 cut(s) 418, 502
Bme18I GGWCC 1 cut(s) 485
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 455
BmsI GCATC 2 cut(s) 270, 376
BmtI GCTAGC 1 cut(s) 317
BplI GAGNNNNNCTC 4 cut(s) 130, 162, 269, 301
Bpu1102I GCTNAGC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 87
Bsa29I ATCGAT 1 cut(s) 523
BsaJI CCNNGG 3 cut(s) 93, 242, 449
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse1I ACTGG 1 cut(s) 510
Bse3DI GCAATG 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 523
BseDI CCNNGG 3 cut(s) 93, 242, 449
BseGI GGATG 2 cut(s) 297, 469
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMI GCAATG 1 cut(s) 179
BseNI ACTGG 1 cut(s) 510
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 2 cut(s) 428, 512
BseYI CCCAGC 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 200
BshVI ATCGAT 1 cut(s) 523
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 243
BsmFI GGGAC 1 cut(s) 77
Bsp143I GATC 3 cut(s) 67, 520, 524
Bsp1720I GCTNAGC 1 cut(s) 11
Bsp19I CCATGG 2 cut(s) 93, 242
BspACI CCGC 3 cut(s) 200, 373, 457
BspDI ATCGAT 1 cut(s) 523
BspFNI CGCG 1 cut(s) 200
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 455
BspMAI CTGCAG 1 cut(s) 307
BspMI ACCTGC 1 cut(s) 220
BspOI GCTAGC 1 cut(s) 317
BspPI GGATC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 179
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 3 cut(s) 93, 242, 449
BssMI GATC 3 cut(s) 67, 520, 524
BssT1I CCWWGG 3 cut(s) 93, 242, 449
Bst6I CTCTTC 2 cut(s) 131, 270
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 2 cut(s) 11, 491
BstDSI CCRYGG 2 cut(s) 93, 242
BstF5I GGATG 2 cut(s) 297, 469
BstFNI CGCG 1 cut(s) 200
BstKTI GATC 3 cut(s) 70, 523, 527
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 3 cut(s) 67, 520, 524
BstMWI GCNNNNNNNGC 2 cut(s) 176, 506
BstNSI RCATGY 1 cut(s) 439
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 2 cut(s) 428, 512
Bsu15I ATCGAT 1 cut(s) 523
BsuI GTATCC 1 cut(s) 91
BsuTUI ATCGAT 1 cut(s) 523
BtgI CCRYGG 2 cut(s) 93, 242
BtsCI GGATG 2 cut(s) 297, 469
BtsIMutI CAGTG 1 cut(s) 503
BveI ACCTGC 1 cut(s) 220
Cac8I GCNNGC 1 cut(s) 315
CaiI CAGNNNCTG 1 cut(s) 235
CciI TCATGA 1 cut(s) 355
Cfr13I GGNCC 1 cut(s) 485
ClaI ATCGAT 1 cut(s) 523
Csp6I GTAC 4 cut(s) 214, 388, 410, 514
CviAII CATG 7 cut(s) 94, 243, 260, 271, 288, 356, 436
CviQI GTAC 4 cut(s) 214, 388, 410, 514
DdeI CTNAG 2 cut(s) 11, 491
DpnI GATC 3 cut(s) 69, 522, 526
DpnII GATC 3 cut(s) 67, 520, 524
Eam1104I CTCTTC 2 cut(s) 131, 270
EarI CTCTTC 2 cut(s) 131, 270
Eco130I CCWWGG 3 cut(s) 93, 242, 449
Eco47I GGWCC 1 cut(s) 485
EcoT14I CCWWGG 3 cut(s) 93, 242, 449
ErhI CCWWGG 3 cut(s) 93, 242, 449
FaeI CATG 7 cut(s) 97, 246, 263, 274, 291, 359, 439
FaqI GGGAC 1 cut(s) 77
FatI CATG 7 cut(s) 93, 242, 259, 270, 287, 355, 435
Fnu4HI GCNGC 2 cut(s) 417, 501
FokI GGATG 2 cut(s) 304, 476
Fsp4HI GCNGC 2 cut(s) 417, 501
FspBI CTAG 1 cut(s) 314
GluI GCNGC 2 cut(s) 417, 501
GsaI CCCAGC 1 cut(s) 463
Hin1II CATG 7 cut(s) 97, 246, 263, 274, 291, 359, 439
HinfI GANTC 4 cut(s) 83, 139, 154, 359
HphI GGTGA 1 cut(s) 362
Hpy188III TCNNGA 3 cut(s) 104, 125, 356
HpyAV CCTTC 1 cut(s) 81
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 3 cut(s) 179, 305, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 506
HpyF3I CTNAG 2 cut(s) 11, 491
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 7 cut(s) 97, 246, 263, 274, 291, 359, 439
Kzo9I GATC 3 cut(s) 67, 520, 524
LmnI GCTCC 1 cut(s) 497
LpnPI CCDG 7 cut(s) 20, 95, 215, 409, 445, 457, 491
Lsp1109I GCAGC 2 cut(s) 428, 512
LweI GCATC 2 cut(s) 270, 376
MaeI CTAG 1 cut(s) 314
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 1 cut(s) 322
MalI GATC 3 cut(s) 69, 522, 526
MboI GATC 3 cut(s) 67, 520, 524
MboII GAAGA 2 cut(s) 148, 287
MluCI AATT 3 cut(s) 20, 28, 248
MlyI GAGTC 2 cut(s) 148, 163
MnlI CCTC 4 cut(s) 86, 106, 159, 498
MspA1I CMGCKG 1 cut(s) 459
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 2 cut(s) 176, 506
NcoI CCATGG 2 cut(s) 93, 242
NdeII GATC 3 cut(s) 67, 520, 524
NheI GCTAGC 1 cut(s) 313
NlaIII CATG 7 cut(s) 97, 246, 263, 274, 291, 359, 439
NlaIV GGNNCC 1 cut(s) 455
NspI RCATGY 1 cut(s) 439
PagI TCATGA 1 cut(s) 355
PciI ACATGT 1 cut(s) 435
PfeI GAWTC 2 cut(s) 83, 359
PkrI GCNGC 2 cut(s) 418, 502
PleI GAGTC 2 cut(s) 147, 162
PpsI GAGTC 2 cut(s) 147, 162
PscI ACATGT 1 cut(s) 435
PspFI CCCAGC 1 cut(s) 459
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 1 cut(s) 485
PstI CTGCAG 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 235
RsaI GTAC 4 cut(s) 215, 389, 411, 515
RsaNI GTAC 4 cut(s) 214, 388, 410, 514
SatI GCNGC 2 cut(s) 417, 501
Sau3AI GATC 3 cut(s) 67, 520, 524
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 2 cut(s) 148, 163
SetI ASST 9 cut(s) 12, 148, 234, 319, 340, 421, 455, 490, 519
SfaNI GCATC 2 cut(s) 270, 376
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 3 cut(s) 20, 28, 248
SsiI CCGC 3 cut(s) 200, 373, 457
SspMI CTAG 1 cut(s) 314
StyI CCWWGG 3 cut(s) 93, 242, 449
TaiI ACGT 1 cut(s) 519
TaqI TCGA 1 cut(s) 523
TasI AATT 3 cut(s) 20, 28, 248
TatI WGTACW 2 cut(s) 213, 409
TfiI GAWTC 2 cut(s) 83, 359
TscAI CASTG 1 cut(s) 510
TseI GCWGC 2 cut(s) 416, 500
TspDTI ATGAA 3 cut(s) 259, 321, 344
TspRI CASTG 1 cut(s) 510
VpaK11BI GGWCC 1 cut(s) 485
XapI RAATTY 1 cut(s) 28
XceI RCATGY 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 456
XspI CTAG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.