pycom15g20580

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005501
Physical Location & Seq
Reverse (-)
14740569 .. 14742319
1751 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1386 bp
ATGCAGTTCAATGCATCAGGTTCCCCGGAATTTATGCATAGAGATACTAAAAGAATTTTGTTAGGGAGACTGAGAAATGTTTCAAAAATAGAAACAGAACTTCATGATCCTTCATTAGTTTCCGGGAGATCTAGGTTATCTGCATTAGACTACGAAAGGGAAAGGGGAGTAGTGAAAGATAAACATGAAAACACAAGATCATTCAGACATGGATCACATGGAGATGAAGTGCTTGATAGAGAGTTGTCCCCTAGAAACCTCATTAGGTCCTTATCCGCCCCAGTACCCGGAACATCTTTTGGGAAACTTCTTCTGGAGGACCGTCATGTTTTAACCGGTGCTCACATTCAGAGGAAGCATGAAGGCATTGGACCTGTGTCCATGGAGATGAAACATCAGAAGAAAGAAAGGTTTAATTTTAAAGAAAAAGTTTCCAATCTCCGATACAGTTTTATGCTTAGGGGGAGACTTTTTGGCAAAAAAAATCAATCAGTTCCCGAATCACGTCATACTGAACGGTATCCTATGAAGGATATCATGAGCGGGCCAACAGTGATTACGAACTCTGGAGAGAGACATGATAATTTCACCGAGGTGCCTCCTAGTCCTGCATCTGTATGCAGCACTGCTCGGGAAGAGTTCTGGAGGCCAACTGATTATCTTAGTCCAATATCAACTCCGAGTGCAACTCCACGAGAAGATGGTATCGTGCCACAGGCTTTTAGGGACATCAGCGATAATCTGAGTGAGCTACGAAGGCAATTGAACCAACTCGAGTCTAATGAGCCTGAGGGGATTAAAGTTGAGGAAGCGGTTGAGTCTGAAATGGCTAGGTTAGACAACCCAGCAGAGGTTTACATACGAGATTTGCTTGTCGTTTCTGGTTTGTACGATGGATCATTTGATAAATCACTATCGAGATGGGACACATTTGCGAAACCAATCAGCAACTCAGTGTTCGAAGAAGTGGAAGAATCGTATAAAAACTTGGCCAACAAGCAAGATCCTTCAGCAACAGATCACAATGAGATGGTAAATCACAAGGTGTTACGCGATTTGTTGAACGAAGCTCTTTCAACAGTTCTCGGACCACCAATAAGCATGTCAAACTTCCGGAGAAAAATGATCAACTCCTCTGCGCTGCCACCATTACGTGGCAAGAAGTTGTTGAATTGTGTGTGGCGAATCATCTGCGAATGCCTATATCCTCCAACTAATGTATCCTGTTACTCACTCGATGATATGGCCGCCCGGGATCTTGGATCAGGTCCTTGGTCCGGATTGATGGACGAGGACGTTAATGCAGTGGGACGAGAGATCGAAAGCTTGATCATGGGAGATCTTGTTCAGGAAATTTTGTATGATATGCAGTTATTAATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 NTPase_1 VARLMGL

Protein Analysis

462

Amino Acids

52.31

Weight (kDa)

5.97

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 285 - 451 1.9e-33 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 595
AccB7I CCANNNNNTGG 1 cut(s) 1154
AccBSI CCGCTC 1 cut(s) 543
AccII CGCG 1 cut(s) 1053
AccIII TCCGGA 2 cut(s) 1113, 1277
AciI CCGC 4 cut(s) 276, 543, 812, 1248
AclWI GGATC 6 cut(s) 101, 220, 904, 998, 1263, 1270
AcoI YGGCCR 2 cut(s) 990, 1245
AcsI RAATTY 3 cut(s) 29, 54, 1353
AcuI CTGAAG 1 cut(s) 993
AdeI CACNNNGTG 1 cut(s) 1045
AfaI GTAC 2 cut(s) 285, 890
AfiI CCNNNNNNNGG 4 cut(s) 287, 850, 1154, 1277
AgeI ACCGGT 1 cut(s) 335
AgsI TTSAA 6 cut(s) 10, 84, 766, 1063, 1077, 1171
AjiI CACGTC 1 cut(s) 506
AleI CACNNNNGTG 1 cut(s) 593
AloI GAACNNNNNNTCC 2 cut(s) 1329, 1361
AluBI AGCT 3 cut(s) 751, 1070, 1326
AluI AGCT 3 cut(s) 751, 1070, 1326
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 3 cut(s) 61, 460, 568
AlwI GGATC 6 cut(s) 101, 220, 904, 998, 1263, 1270
Ama87I CYCGRG 3 cut(s) 630, 773, 1251
Aor13HI TCCGGA 2 cut(s) 1113, 1277
AoxI GGCC 4 cut(s) 545, 647, 990, 1245
ApeKI GCWGC 2 cut(s) 621, 1141
ApoI RAATTY 3 cut(s) 29, 54, 1353
AseI ATTAAT 1 cut(s) 1376
AsiGI ACCGGT 1 cut(s) 335
Asp700I GAANNNNTTC 1 cut(s) 79
AspLEI GCGC 1 cut(s) 1141
AspS9I GGNCC 7 cut(s) 267, 319, 371, 545, 1088, 1268, 1275
AsuC2I CCSGG 5 cut(s) 26, 124, 288, 1252, 1253
AsuHPI GGTGA 1 cut(s) 580
AsuII TTCGAA 1 cut(s) 960
AvaI CYCGRG 3 cut(s) 630, 773, 1251
AvaII GGWCC 6 cut(s) 267, 319, 371, 1088, 1268, 1275
AxyI CCTNAGG 1 cut(s) 789
BaeI ACNNNNGTAYC 2 cut(s) 1203, 1236
BalI TGGCCA 1 cut(s) 992
BanI GGYRCC 1 cut(s) 595
BauI CACGAG 1 cut(s) 693
Bbv12I GWGCWC 1 cut(s) 343
BbvI GCAGC 2 cut(s) 633, 1128
BccI CCATC 5 cut(s) 695, 887, 915, 1024, 1279
BcgI CGANNNNNNTGC 2 cut(s) 1173, 1207
BciVI GTATCC 2 cut(s) 531, 1231
BclI TGATCA 2 cut(s) 1125, 1329
BcnI CCSGG 5 cut(s) 26, 124, 288, 1252, 1253
BcoDI GTCTC 3 cut(s) 61, 460, 568
BfaI CTAG 4 cut(s) 132, 252, 603, 831
BfuI GTATCC 2 cut(s) 531, 1231
BglII AGATCT 2 cut(s) 128, 1339
BisI GCNGC 3 cut(s) 622, 1142, 1248
BlsI GCNGC 3 cut(s) 623, 1143, 1249
Bme1390I CCNGG 5 cut(s) 26, 124, 288, 1252, 1253
Bme18I GGWCC 6 cut(s) 267, 319, 371, 1088, 1268, 1275
BmeT110I CYCGRG 3 cut(s) 630, 773, 1251
BmgBI CACGTC 1 cut(s) 506
BmgT120I GGNCC 7 cut(s) 267, 319, 371, 545, 1088, 1268, 1275
BmiI GGNNCC 2 cut(s) 22, 597
BmrFI CCNGG 5 cut(s) 26, 124, 288, 1252, 1253
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 2 cut(s) 23, 620
BmuI ACTGGG 1 cut(s) 275
BoxI GACNNNNGTC 1 cut(s) 376
BplI GAGNNNNNCTC 2 cut(s) 673, 705
BpmI CTGGAG 3 cut(s) 335, 588, 664
Bpu10I CCTNAGC 1 cut(s) 458
Bpu14I TTCGAA 1 cut(s) 960
BpuMI CCSGG 5 cut(s) 26, 124, 288, 1252, 1253
BsaAI YACGTR 1 cut(s) 1154
BsaJI CCNNGG 5 cut(s) 24, 381, 591, 1251, 1271
BsaWI WCCGGW 3 cut(s) 335, 1113, 1277
BsaXI ACNNNNNCTCC 4 cut(s) 213, 243, 1329, 1359
Bsc4I CCNNNNNNNGG 4 cut(s) 287, 850, 1154, 1277
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 1 cut(s) 281
Bse21I CCTNAGG 1 cut(s) 789
BseAI TCCGGA 2 cut(s) 1113, 1277
BseDI CCNNGG 5 cut(s) 24, 381, 591, 1251, 1271
BseLI CCNNNNNNNGG 4 cut(s) 287, 850, 1154, 1277
BseMII CTCAG 4 cut(s) 62, 734, 780, 966
BseNI ACTGG 1 cut(s) 281
BseRI GAGGAG 1 cut(s) 1123
BseXI GCAGC 2 cut(s) 633, 1128
BseYI CCCAGC 1 cut(s) 844
Bsh1236I CGCG 1 cut(s) 1053
BshFI GGCC 4 cut(s) 547, 649, 992, 1247
BshNI GGYRCC 1 cut(s) 595
BshTI ACCGGT 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 343
BsiHKCI CYCGRG 3 cut(s) 630, 773, 1251
BsiSI CCGG 7 cut(s) 26, 123, 288, 336, 1114, 1252, 1278
BslFI GGGAC 4 cut(s) 232, 740, 938, 1323
BslI CCNNNNNNNGG 4 cut(s) 287, 850, 1154, 1277
BsmAI GTCTC 3 cut(s) 61, 460, 568
BsmFI GGGAC 4 cut(s) 232, 740, 938, 1323
BsmI GAATGC 1 cut(s) 1202
BsnI GGCC 4 cut(s) 547, 649, 992, 1247
BsoBI CYCGRG 3 cut(s) 630, 773, 1251
Bsp119I TTCGAA 1 cut(s) 960
Bsp1286I GDGCHC 1 cut(s) 343
Bsp13I TCCGGA 2 cut(s) 1113, 1277
Bsp19I CCATGG 1 cut(s) 381
BspACI CCGC 4 cut(s) 276, 543, 812, 1248
BspANI GGCC 4 cut(s) 547, 649, 992, 1247
BspCNI CTCAG 4 cut(s) 63, 735, 781, 965
BspEI TCCGGA 2 cut(s) 1113, 1277
BspFNI CGCG 1 cut(s) 1053
BspHI TCATGA 2 cut(s) 103, 537
BspLI GGNNCC 2 cut(s) 22, 597
BspPI GGATC 6 cut(s) 101, 220, 904, 998, 1263, 1270
BspT104I TTCGAA 1 cut(s) 960
BspT107I GGYRCC 1 cut(s) 595
BsrBI CCGCTC 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 335
BsrI ACTGG 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 5 cut(s) 24, 381, 591, 1251, 1271
BssSI CACGAG 1 cut(s) 693
BssT1I CCWWGG 2 cut(s) 381, 1271
Bst2BI CACGAG 1 cut(s) 693
Bst4CI ACNGT 5 cut(s) 323, 449, 519, 553, 1081
Bst6I CTCTTC 1 cut(s) 630
BstBAI YACGTR 1 cut(s) 1154
BstBI TTCGAA 1 cut(s) 960
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 6 cut(s) 71, 458, 662, 743, 789, 952
BstDSI CCRYGG 1 cut(s) 381
BstFNI CGCG 1 cut(s) 1053
BstHHI GCGC 1 cut(s) 1141
BstMAI GTCTC 3 cut(s) 61, 460, 568
BstMWI GCNNNNNNNGC 1 cut(s) 757
BstNSI RCATGY 1 cut(s) 1105
BstPAI GACNNNNGTC 1 cut(s) 376
BstSCI CCNGG 5 cut(s) 24, 122, 286, 1250, 1251
BstUI CGCG 1 cut(s) 1053
BstV1I GCAGC 2 cut(s) 633, 1128
BstX2I RGATCY 4 cut(s) 128, 1003, 1255, 1339
BstYI RGATCY 4 cut(s) 128, 1003, 1255, 1339
Bsu36I CCTNAGG 1 cut(s) 789
BsuI GTATCC 2 cut(s) 531, 1231
BsuRI GGCC 4 cut(s) 547, 649, 992, 1247
BtgI CCRYGG 1 cut(s) 381
BtrI CACGTC 1 cut(s) 506
BtsI GCAGTG 2 cut(s) 624, 1311
BtsIMutI CAGTG 4 cut(s) 558, 624, 960, 1311
Cac8I GCNNGC 1 cut(s) 545
CciI TCATGA 2 cut(s) 103, 537
CfoI GCGC 1 cut(s) 1141
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 7 cut(s) 267, 319, 371, 545, 1088, 1268, 1275
Cfr9I CCCGGG 1 cut(s) 1251
Csp6I GTAC 2 cut(s) 284, 889
CspAI ACCGGT 1 cut(s) 335
CviQI GTAC 2 cut(s) 284, 889
DdeI CTNAG 6 cut(s) 71, 458, 662, 743, 789, 952
DraI TTTAAA 1 cut(s) 421
DraIII CACNNNGTG 1 cut(s) 1045
EaeI YGGCCR 2 cut(s) 990, 1245
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
EciI GGCGGA 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 381, 1271
Eco32I GATATC 1 cut(s) 535
Eco47I GGWCC 6 cut(s) 267, 319, 371, 1088, 1268, 1275
Eco57I CTGAAG 1 cut(s) 993
Eco81I CCTNAGG 1 cut(s) 789
Eco88I CYCGRG 3 cut(s) 630, 773, 1251
EcoO109I RGGNCCY 2 cut(s) 267, 1268
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 2 cut(s) 381, 1271
EcoT22I ATGCAT 2 cut(s) 16, 39
ErhI CCWWGG 2 cut(s) 381, 1271
FaqI GGGAC 4 cut(s) 232, 740, 938, 1323
FauI CCCGC 1 cut(s) 536
FbaI TGATCA 2 cut(s) 1125, 1329
Fnu4HI GCNGC 3 cut(s) 622, 1142, 1248
Fsp4HI GCNGC 3 cut(s) 622, 1142, 1248
FspBI CTAG 4 cut(s) 132, 252, 603, 831
GlaI GCGC 1 cut(s) 1140
GluI GCNGC 3 cut(s) 622, 1142, 1248
GsaI CCCAGC 1 cut(s) 848
GsuI CTGGAG 3 cut(s) 335, 588, 664
HaeIII GGCC 4 cut(s) 547, 649, 992, 1247
HapII CCGG 7 cut(s) 26, 123, 288, 336, 1114, 1252, 1278
HhaI GCGC 1 cut(s) 1141
Hin6I GCGC 1 cut(s) 1139
HinP1I GCGC 1 cut(s) 1139
HindIII AAGCTT 1 cut(s) 1324
HinfI GANTC 5 cut(s) 500, 776, 818, 974, 1185
HpaII CCGG 7 cut(s) 26, 123, 288, 336, 1114, 1252, 1278
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 8 cut(s) 206, 351, 399, 443, 681, 744, 823, 1088
Hpy8I GTNNAC 1 cut(s) 856
HpyAV CCTTC 5 cut(s) 120, 356, 523, 750, 1017
HpyCH4III ACNGT 5 cut(s) 323, 449, 519, 553, 1081
HpyCH4IV ACGT 3 cut(s) 505, 1153, 1296
HpyCH4V TGCA 9 cut(s) 4, 14, 37, 143, 611, 621, 686, 1304, 1369
HpyF10VI GCNNNNNNNGC 1 cut(s) 757
HpyF3I CTNAG 6 cut(s) 71, 458, 662, 743, 789, 952
HpySE526I ACGT 3 cut(s) 505, 1153, 1296
HspAI GCGC 1 cut(s) 1139
Kpn2I TCCGGA 2 cut(s) 1113, 1277
Ksp22I TGATCA 2 cut(s) 1125, 1329
Lsp1109I GCAGC 2 cut(s) 633, 1128
LweI GCATC 2 cut(s) 23, 620
MaeI CTAG 4 cut(s) 132, 252, 603, 831
MaeII ACGT 3 cut(s) 505, 1153, 1296
MaeIII GTNAC 2 cut(s) 1047, 1226
MbiI CCGCTC 1 cut(s) 543
MboII GAAGA 6 cut(s) 302, 412, 647, 710, 974, 983
MfeI CAATTG 1 cut(s) 761
MflI RGATCY 4 cut(s) 128, 1003, 1255, 1339
MhlI GDGCHC 1 cut(s) 343
MlsI TGGCCA 1 cut(s) 992
MluCI AATT 7 cut(s) 29, 54, 415, 583, 761, 1171, 1353
MluNI TGGCCA 1 cut(s) 992
MlyI GAGTC 2 cut(s) 785, 827
MmeI TCCRAC 1 cut(s) 1235
Mox20I TGGCCA 1 cut(s) 992
Mph1103I ATGCAT 2 cut(s) 16, 39
MroI TCCGGA 2 cut(s) 1113, 1277
MroXI GAANNNNTTC 1 cut(s) 79
MscI TGGCCA 1 cut(s) 992
MseI TTAA 6 cut(s) 332, 414, 420, 798, 1299, 1376
MslI CAYNNNNRTG 4 cut(s) 222, 386, 593, 616
Msp20I TGGCCA 1 cut(s) 992
MspI CCGG 7 cut(s) 26, 123, 288, 336, 1114, 1252, 1278
MspR9I CCNGG 5 cut(s) 26, 124, 288, 1252, 1253
MunI CAATTG 1 cut(s) 761
Mva1269I GAATGC 1 cut(s) 1202
MvnI CGCG 1 cut(s) 1053
MwoI GCNNNNNNNGC 1 cut(s) 757
NciI CCSGG 5 cut(s) 26, 124, 288, 1252, 1253
NcoI CCATGG 1 cut(s) 381
NlaIV GGNNCC 2 cut(s) 22, 597
NsiI ATGCAT 2 cut(s) 16, 39
NspI RCATGY 1 cut(s) 1105
NspV TTCGAA 1 cut(s) 960
OliI CACNNNNGTG 1 cut(s) 593
PaeR7I CTCGAG 1 cut(s) 773
PagI TCATGA 2 cut(s) 103, 537
PctI GAATGC 1 cut(s) 1202
PdmI GAANNNNTTC 1 cut(s) 79
PfeI GAWTC 3 cut(s) 500, 974, 1185
PflMI CCANNNNNTGG 1 cut(s) 1154
PfoI TCCNGGA 1 cut(s) 122
PinAI ACCGGT 1 cut(s) 335
PkrI GCNGC 3 cut(s) 623, 1143, 1249
PleI GAGTC 2 cut(s) 784, 826
PpsI GAGTC 2 cut(s) 784, 826
Ppu21I YACGTR 1 cut(s) 1154
PpuMI RGGWCCY 2 cut(s) 267, 1268
PshAI GACNNNNGTC 1 cut(s) 376
PshBI ATTAAT 1 cut(s) 1376
Psp5II RGGWCCY 2 cut(s) 267, 1268
PspFI CCCAGC 1 cut(s) 844
PspN4I GGNNCC 2 cut(s) 22, 597
PspPI GGNCC 7 cut(s) 267, 319, 371, 545, 1088, 1268, 1275
PspPPI RGGWCCY 2 cut(s) 267, 1268
PspXI VCTCGAGB 1 cut(s) 773
PsuI RGATCY 4 cut(s) 128, 1003, 1255, 1339
RsaI GTAC 2 cut(s) 285, 890
RsaNI GTAC 2 cut(s) 284, 889
RseI CAYNNNNRTG 4 cut(s) 222, 386, 593, 616
SaqAI TTAA 6 cut(s) 332, 414, 420, 798, 1299, 1376
SatI GCNGC 3 cut(s) 622, 1142, 1248
Sau96I GGNCC 7 cut(s) 267, 319, 371, 545, 1088, 1268, 1275
SchI GAGTC 2 cut(s) 785, 827
ScrFI CCNGG 5 cut(s) 26, 124, 288, 1252, 1253
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 2 cut(s) 23, 620
Sfr274I CTCGAG 1 cut(s) 773
SfuI TTCGAA 1 cut(s) 960
SinI GGWCC 6 cut(s) 267, 319, 371, 1088, 1268, 1275
SlaI CTCGAG 1 cut(s) 773
SmaI CCCGGG 1 cut(s) 1253
SmiMI CAYNNNNRTG 4 cut(s) 222, 386, 593, 616
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
Sse9I AATT 7 cut(s) 29, 54, 415, 583, 761, 1171, 1353
SsiI CCGC 4 cut(s) 276, 543, 812, 1248
SspMI CTAG 4 cut(s) 132, 252, 603, 831
StyD4I CCNGG 5 cut(s) 24, 122, 286, 1250, 1251
StyI CCWWGG 2 cut(s) 381, 1271
TaaI ACNGT 5 cut(s) 323, 449, 519, 553, 1081
TaiI ACGT 3 cut(s) 508, 1156, 1299
TaqI TCGA 5 cut(s) 774, 917, 960, 1236, 1320
TasI AATT 7 cut(s) 29, 54, 415, 583, 761, 1171, 1353
TauI GCSGC 1 cut(s) 1250
TfiI GAWTC 3 cut(s) 500, 974, 1185
Tru1I TTAA 6 cut(s) 332, 414, 420, 798, 1299, 1376
Tru9I TTAA 6 cut(s) 332, 414, 420, 798, 1299, 1376
TscAI CASTG 4 cut(s) 558, 631, 960, 1311
TseI GCWGC 2 cut(s) 621, 1141
TspDTI ATGAA 7 cut(s) 92, 102, 201, 240, 375, 404, 542
TspMI CCCGGG 1 cut(s) 1251
TspRI CASTG 4 cut(s) 558, 631, 960, 1311
Van91I CCANNNNNTGG 1 cut(s) 1154
VpaK11BI GGWCC 6 cut(s) 267, 319, 371, 1088, 1268, 1275
VspI ATTAAT 1 cut(s) 1376
XapI RAATTY 3 cut(s) 29, 54, 1353
XceI RCATGY 1 cut(s) 1105
XhoI CTCGAG 1 cut(s) 773
XmaI CCCGGG 1 cut(s) 1251
XmnI GAANNNNTTC 1 cut(s) 79
XspI CTAG 4 cut(s) 132, 252, 603, 831
Zsp2I ATGCAT 2 cut(s) 16, 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.