pycom15g23840

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013560
Physical Location & Seq
Forward (+)
17981120 .. 17984390
3271 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
ATGGCGTTCTCGTCATTATTCTCCACGGCGCAGCCCCAACAGCAGCAGCAGCAATCGCTGTTTCAAACTCAACAGGCCTCGCAGCCTTTTCAGCAAAGCAGCTCCTTTTTCTCCCAACAACCACTGCAGCAGCAGCCGCCGCTGTTCCCACAGCCGCAATTTCAGCAGCAGCCACTGTTTCAACAGCCTCAATTTCAGCAGCAGCAACAGCAGCAGCAATTGTACTTGTTTACGAACGAGAAGACTCCGGCGAGTTATGGTACCAAGTGGGCGGATCTCCATCCGGATTCCCAGAAAGTTCTTCTGCAAATTGAGGAACGGATACTGGGATATAGGGATGAAAGCCAGCGGCTGGATCAGTGTAGTCGCCTCTGCGATTCATCAGTTTCGAATGATGCTTTTGAGCATGACGCAAGTCGTATTCTCCAGGAACTTGGGGGTATTGGTACTTCCATGGACCGAGAGAAAGCTGTTCTGCACGAGCTGATGGCTATTGTGAAAGATATGTTGCGGAACAGCGAGGTTGCTGTTCGTTCTTTCATGATTCTACGCCCAAGGTTTCTTCATCCAAATGCAGGAGGTACTTCAAATGCTACTGCCCCATCACAAGCTCCTGGAGCAACAGTACCACCAGGTTCAAGTAGTCAACCAACAACTACTTCTATAGTGCCAGTTTTTGATTTTTACAATGGGATTCCAAGGAAACCATCTCCCTTCCTACAGCAAACAATGGCAAGATTTGAGAAGTATCTTGTTGAGTGTCGCCAGTGGATTGAAGAATTAGAGCAGCTGCTTCTGGACTCAGAGAGGAACTCTGCCAATGATAGATCCTCATTGTTGCAGTCTCTTCCAAATGTTATGAAAAATGTGCATGATTTTTTTGTTCACGTGGCAGCTAAGGTGGAGAGTATTCATCAATACGTTGTGTCCATGAAAACAGCTTATCTTGTTAACCAGCGCCGCCAAGGGGATGGGAATGATCCATTTCTCGAGGCTGATCGGCGGGAAACTGCTAGGCAAGAAGCTGCTGCTAAGAGGGCGCATCCAACTTTGCATTTATCTGCAACTTCGCAACCATCAACCCAAGTTGCTGGGTTGTTTGCCAGTTCAGGAACTCCTGGGACATCGACTGCCCCACAGACATCTGCAGCTACCATTACAGCTTCATCTGGGAGTGGACTTTTCAGTACCCCTTCTGCTCCATCTGCTTCCACGTCTTCCTCTCTGTTTGCTACACCACCCACTTCGGGTCCTGCATCTTCTCTTTTTTCAACACCGTCTGCTACACCCCAGGCATCACTGATTAGTTCTTCATCAGCGTTTGGACCTGCTTCAACTCCCTCTCTGTTTTCCAATCCTACTCCAGGCTTCAGTTCTACTACTCCTATTGGAAGTTCATTATTTACTTCAGGTAGTGCTGGATCAGGGCTGAATTCTAGCTTTACTTCAACGAAATCAGCACGGCCGAGAAGTCGGACTGGTGGCCGCCGTTAG

Protein Analysis

498

Amino Acids

53.93

Weight (kDa)

8.73

Isoelectric Point (pI)

65.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013560)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37130
fragaria_vesca FvH4_1g14810
malus_domestica MD02G1161300.v1.1 MD15G1275400.v1.1
prunus_persica Prupe.7G142300_v2.0.a1
pyrus_communis pycom02g12740 pycom15g23840
rosa_chinensis RchiOBHm_Chr2g0103771
rosa_laevigata RLG00000017258
rosa_multiflora Rmu_co8256557.1_g000001
rosa_roxburghii Rroxscaffold_2G00139410
rosa_rugosa Rorug02G0117200
rosa_samantha Rh2AG166200 Rh2BG173400 Rh2CG172100 Rh2DG171600
rosa_wichuraiana Rw0G006920 Rw2G013020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1336
Acc65I GGTACC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 352
AccIII TCCGGA 1 cut(s) 283
AciI CCGC 9 cut(s) 137, 140, 155, 272, 349, 511, 961, 1003, 1486
AclWI GGATC 5 cut(s) 282, 363, 822, 974, 1429
AcoI YGGCCR 2 cut(s) 1463, 1483
AcsI RAATTY 1 cut(s) 1432
AcuI CTGAAG 2 cut(s) 1354, 1392
AcvI CACGTG 1 cut(s) 889
AfaI GTAC 6 cut(s) 224, 262, 448, 583, 627, 1189
AfiI CCNNNNNNNGG 5 cut(s) 352, 575, 967, 1247, 1364
AgsI TTSAA 8 cut(s) 65, 182, 588, 639, 776, 1272, 1335, 1449
AjiI CACGTC 1 cut(s) 1215
AjnI CCWGG 6 cut(s) 426, 613, 631, 1117, 1290, 1363
AjuI GAANNNNNNNTTGG 2 cut(s) 643, 675
Alw26I GTCTC 1 cut(s) 849
AlwI GGATC 5 cut(s) 282, 363, 822, 974, 1429
AlwNI CAGNNNCTG 2 cut(s) 175, 352
Ama87I CYCGRG 1 cut(s) 989
Aor13HI TCCGGA 1 cut(s) 283
AoxI GGCC 3 cut(s) 75, 1463, 1483
ApoI RAATTY 1 cut(s) 1432
Asp718I GGTACC 1 cut(s) 260
AspLEI GCGC 3 cut(s) 31, 960, 1042
AspS9I GGNCC 3 cut(s) 457, 1250, 1325
AsuII TTCGAA 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 989
AvaII GGWCC 3 cut(s) 457, 1250, 1325
BanI GGYRCC 1 cut(s) 260
BarI GAAGNNNNNNTAC 2 cut(s) 433, 465
BauI CACGAG 1 cut(s) 479
BbrPI CACGTG 1 cut(s) 889
BbsI GAAGAC 2 cut(s) 248, 1209
BccI CCATC 7 cut(s) 288, 481, 610, 715, 965, 1084, 1210
BceAI ACGGC 3 cut(s) 42, 1473, 1478
BciT130I CCWGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
BciVI GTATCC 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 849
BfaI CTAG 2 cut(s) 1014, 1437
BfmI CTRYAG 4 cut(s) 125, 663, 719, 1146
BfoI RGCGCY 1 cut(s) 961
BfuAI ACCTGC 1 cut(s) 1336
BfuI GTATCC 1 cut(s) 315
Bme1390I CCNGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
Bme18I GGWCC 3 cut(s) 457, 1250, 1325
BmeT110I CYCGRG 1 cut(s) 989
BmgBI CACGTC 1 cut(s) 1215
BmgT120I GGNCC 3 cut(s) 457, 1250, 1325
BmiI GGNNCC 2 cut(s) 262, 1251
BmrFI CCNGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
BmrI ACTGGG 1 cut(s) 335
BmsI GCATC 4 cut(s) 385, 1051, 1265, 1304
BmuI ACTGGG 1 cut(s) 335
BoxI GACNNNNGTC 1 cut(s) 414
BpiI GAAGAC 2 cut(s) 248, 1209
BplI GAGNNNNNCTC 2 cut(s) 797, 829
BpmI CTGGAG 3 cut(s) 410, 636, 1347
Bpu10I CCTNAGC 1 cut(s) 897
Bpu14I TTCGAA 1 cut(s) 389
BsaAI YACGTR 1 cut(s) 889
BsaBI GATNNNNATC 1 cut(s) 279
BsaJI CCNNGG 7 cut(s) 24, 453, 554, 698, 964, 1118, 1290
BsaWI WCCGGW 1 cut(s) 283
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bsc4I CCNNNNNNNGG 5 cut(s) 352, 575, 967, 1247, 1364
Bse1I ACTGG 5 cut(s) 330, 671, 766, 1104, 1483
Bse8I GATNNNNATC 1 cut(s) 279
BseAI TCCGGA 1 cut(s) 283
BseBI CCWGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
BseDI CCNNGG 7 cut(s) 24, 453, 554, 698, 964, 1118, 1290
BseGI GGATG 5 cut(s) 280, 343, 565, 976, 1042
BseJI GATNNNNATC 1 cut(s) 279
BseLI CCNNNNNNNGG 5 cut(s) 352, 575, 967, 1247, 1364
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 5 cut(s) 330, 671, 766, 1104, 1483
BseX3I CGGCCG 1 cut(s) 1463
BseYI CCCAGC 1 cut(s) 1091
BsgI GTGCAG 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 1466
BshFI GGCC 3 cut(s) 77, 1465, 1485
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 1466
BsiHKCI CYCGRG 1 cut(s) 989
BsiSI CCGG 2 cut(s) 248, 284
BslFI GGGAC 1 cut(s) 1135
BslI CCNNNNNNNGG 5 cut(s) 352, 575, 967, 1247, 1364
BsmAI GTCTC 1 cut(s) 849
BsmFI GGGAC 1 cut(s) 1135
BsnI GGCC 3 cut(s) 77, 1465, 1485
BsoBI CYCGRG 1 cut(s) 989
Bsp119I TTCGAA 1 cut(s) 389
Bsp13I TCCGGA 1 cut(s) 283
Bsp143I GATC 6 cut(s) 274, 355, 827, 979, 997, 1421
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 9 cut(s) 137, 140, 155, 272, 349, 511, 961, 1003, 1486
BspANI GGCC 3 cut(s) 77, 1465, 1485
BspCNI CTCAG 1 cut(s) 815
BspEI TCCGGA 1 cut(s) 283
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 2 cut(s) 262, 1251
BspMAI CTGCAG 2 cut(s) 129, 1150
BspMI ACCTGC 1 cut(s) 1336
BspPI GGATC 5 cut(s) 282, 363, 822, 974, 1429
BspT104I TTCGAA 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 260
BsrI ACTGG 5 cut(s) 330, 671, 766, 1104, 1483
BssECI CCNNGG 7 cut(s) 24, 453, 554, 698, 964, 1118, 1290
BssMI GATC 6 cut(s) 274, 355, 827, 979, 997, 1421
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 4 cut(s) 453, 554, 698, 964
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
Bst4CI ACNGT 3 cut(s) 177, 625, 1278
Bst6I CTCTTC 1 cut(s) 852
BstBAI YACGTR 1 cut(s) 889
BstBI TTCGAA 1 cut(s) 389
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 3 cut(s) 802, 897, 1032
BstDSI CCRYGG 2 cut(s) 24, 453
BstENI CCTNNNNNAGG 1 cut(s) 1362
BstF5I GGATG 5 cut(s) 280, 343, 565, 976, 1042
BstH2I RGCGCY 1 cut(s) 961
BstHHI GCGC 3 cut(s) 31, 960, 1042
BstKTI GATC 6 cut(s) 277, 358, 830, 982, 1000, 1424
BstMAI GTCTC 1 cut(s) 849
BstMBI GATC 6 cut(s) 274, 355, 827, 979, 997, 1421
BstMCI CGRYCG 1 cut(s) 1466
BstNI CCWGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
BstPAI GACNNNNGTC 1 cut(s) 414
BstSCI CCNGG 6 cut(s) 426, 613, 631, 1117, 1290, 1363
BstSFI CTRYAG 4 cut(s) 125, 663, 719, 1146
BstV2I GAAGAC 2 cut(s) 248, 1209
BstX2I RGATCY 2 cut(s) 274, 827
BstXI CCANNNNNNTGG 3 cut(s) 434, 971, 1091
BstYI RGATCY 2 cut(s) 274, 827
BstZI CGGCCG 1 cut(s) 1463
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 3 cut(s) 77, 1465, 1485
BtgI CCRYGG 2 cut(s) 24, 453
BtrI CACGTC 1 cut(s) 1215
BtsCI GGATG 5 cut(s) 280, 343, 565, 976, 1042
BtsI GCAGTG 1 cut(s) 122
BtsIMutI CAGTG 5 cut(s) 122, 173, 365, 773, 1298
BveI ACCTGC 1 cut(s) 1336
Cac8I GCNNGC 1 cut(s) 347
CaiI CAGNNNCTG 2 cut(s) 175, 352
CciI TCATGA 1 cut(s) 540
CfoI GCGC 3 cut(s) 31, 960, 1042
Cfr13I GGNCC 3 cut(s) 457, 1250, 1325
CseI GACGC 1 cut(s) 419
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 6 cut(s) 223, 261, 447, 582, 626, 1188
CviAII CATG 5 cut(s) 407, 454, 541, 872, 931
CviQI GTAC 6 cut(s) 223, 261, 447, 582, 626, 1188
DdeI CTNAG 3 cut(s) 802, 897, 1032
DpnI GATC 6 cut(s) 276, 357, 829, 981, 999, 1423
DpnII GATC 6 cut(s) 274, 355, 827, 979, 997, 1421
EaeI YGGCCR 2 cut(s) 1463, 1483
EagI CGGCCG 1 cut(s) 1463
Eam1104I CTCTTC 1 cut(s) 852
EarI CTCTTC 1 cut(s) 852
EciI GGCGGA 1 cut(s) 287
EclXI CGGCCG 1 cut(s) 1463
Eco130I CCWWGG 4 cut(s) 453, 554, 698, 964
Eco147I AGGCCT 1 cut(s) 77
Eco47I GGWCC 3 cut(s) 457, 1250, 1325
Eco52I CGGCCG 1 cut(s) 1463
Eco57I CTGAAG 2 cut(s) 1354, 1392
Eco72I CACGTG 1 cut(s) 889
Eco88I CYCGRG 1 cut(s) 989
EcoNI CCTNNNNNAGG 1 cut(s) 1362
EcoO109I RGGNCCY 1 cut(s) 1250
EcoRI GAATTC 1 cut(s) 1432
EcoRII CCWGG 6 cut(s) 426, 613, 631, 1117, 1290, 1363
EcoT14I CCWWGG 4 cut(s) 453, 554, 698, 964
ErhI CCWWGG 4 cut(s) 453, 554, 698, 964
FaeI CATG 5 cut(s) 410, 457, 544, 875, 934
FaqI GGGAC 1 cut(s) 1135
FatI CATG 5 cut(s) 406, 453, 540, 871, 930
FauI CCCGC 1 cut(s) 996
FokI GGATG 5 cut(s) 267, 350, 552, 983, 1029
FspBI CTAG 2 cut(s) 1014, 1437
GlaI GCGC 3 cut(s) 30, 959, 1041
GsaI CCCAGC 1 cut(s) 1095
GsuI CTGGAG 3 cut(s) 410, 636, 1347
HaeII RGCGCY 1 cut(s) 961
HaeIII GGCC 3 cut(s) 77, 1465, 1485
HapII CCGG 2 cut(s) 248, 284
HgaI GACGC 1 cut(s) 419
HhaI GCGC 3 cut(s) 31, 960, 1042
Hin1II CATG 5 cut(s) 410, 457, 544, 875, 934
Hin6I GCGC 3 cut(s) 29, 958, 1040
HinP1I GCGC 3 cut(s) 29, 958, 1040
HincII GTYRAC 2 cut(s) 647, 952
HindII GTYRAC 2 cut(s) 647, 952
HinfI GANTC 6 cut(s) 244, 287, 377, 544, 694, 800
HpaI GTTAAC 1 cut(s) 952
HpaII CCGG 2 cut(s) 248, 284
Hpy166II GTNNAC 5 cut(s) 231, 647, 886, 952, 1178
Hpy188I TCNGA 2 cut(s) 805, 1476
Hpy188III TCNNGA 5 cut(s) 284, 541, 797, 989, 1110
Hpy8I GTNNAC 5 cut(s) 231, 647, 886, 952, 1178
HpyAV CCTTC 2 cut(s) 724, 1203
HpyCH4III ACNGT 3 cut(s) 177, 625, 1278
HpyCH4IV ACGT 3 cut(s) 888, 921, 1214
HpyF3I CTNAG 3 cut(s) 802, 897, 1032
HpySE526I ACGT 3 cut(s) 888, 921, 1214
Hsp92II CATG 5 cut(s) 410, 457, 544, 875, 934
HspAI GCGC 3 cut(s) 29, 958, 1040
Kpn2I TCCGGA 1 cut(s) 283
KpnI GGTACC 1 cut(s) 264
KspAI GTTAAC 1 cut(s) 952
Kzo9I GATC 6 cut(s) 274, 355, 827, 979, 997, 1421
LmnI GCTCC 4 cut(s) 107, 616, 617, 1204
LweI GCATC 4 cut(s) 385, 1051, 1265, 1304
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 2 cut(s) 1014, 1437
MaeII ACGT 3 cut(s) 888, 921, 1214
MalI GATC 6 cut(s) 276, 357, 829, 981, 999, 1423
MboI GATC 6 cut(s) 274, 355, 827, 979, 997, 1421
MboII GAAGA 8 cut(s) 253, 293, 554, 788, 839, 1209, 1251, 1302
MfeI CAATTG 1 cut(s) 218
MflI RGATCY 2 cut(s) 274, 827
MluCI AATT 6 cut(s) 158, 191, 218, 309, 779, 1432
MlyI GAGTC 2 cut(s) 238, 794
MmeI TCCRAC 2 cut(s) 1070, 1454
MroI TCCGGA 1 cut(s) 283
MseI TTAA 1 cut(s) 951
MslI CAYNNNNRTG 1 cut(s) 570
MspA1I CMGCKG 3 cut(s) 142, 349, 790
MspI CCGG 2 cut(s) 248, 284
MspR9I CCNGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
MunI CAATTG 1 cut(s) 218
MvaI CCWGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
NcoI CCATGG 1 cut(s) 453
NdeII GATC 6 cut(s) 274, 355, 827, 979, 997, 1421
NlaIII CATG 5 cut(s) 410, 457, 544, 875, 934
NlaIV GGNNCC 2 cut(s) 262, 1251
NmeAIII GCCGAG 1 cut(s) 1491
NspV TTCGAA 1 cut(s) 389
PaeR7I CTCGAG 1 cut(s) 989
PagI TCATGA 1 cut(s) 540
PceI AGGCCT 1 cut(s) 77
PfeI GAWTC 4 cut(s) 287, 377, 544, 694
PflMI CCANNNNNTGG 1 cut(s) 352
PfoI TCCNGGA 2 cut(s) 426, 613
PleI GAGTC 2 cut(s) 238, 794
PmaCI CACGTG 1 cut(s) 889
PmlI CACGTG 1 cut(s) 889
PpsI GAGTC 2 cut(s) 238, 794
Ppu21I YACGTR 1 cut(s) 889
PpuMI RGGWCCY 1 cut(s) 1250
PshAI GACNNNNGTC 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 1250
Psp6I CCWGG 6 cut(s) 426, 613, 631, 1117, 1290, 1363
PspCI CACGTG 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 1091
PspGI CCWGG 6 cut(s) 426, 613, 631, 1117, 1290, 1363
PspN4I GGNNCC 2 cut(s) 262, 1251
PspPI GGNCC 3 cut(s) 457, 1250, 1325
PspPPI RGGWCCY 1 cut(s) 1250
PstI CTGCAG 2 cut(s) 129, 1150
PstNI CAGNNNCTG 2 cut(s) 175, 352
PsuI RGATCY 2 cut(s) 274, 827
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 6 cut(s) 224, 262, 448, 583, 627, 1189
RsaNI GTAC 6 cut(s) 223, 261, 447, 582, 626, 1188
RseI CAYNNNNRTG 1 cut(s) 570
SaqAI TTAA 1 cut(s) 951
Sau3AI GATC 6 cut(s) 274, 355, 827, 979, 997, 1421
Sau96I GGNCC 3 cut(s) 457, 1250, 1325
SchI GAGTC 2 cut(s) 238, 794
ScrFI CCNGG 6 cut(s) 428, 615, 633, 1119, 1292, 1365
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 4 cut(s) 385, 1051, 1265, 1304
SfcI CTRYAG 4 cut(s) 125, 663, 719, 1146
Sfr274I CTCGAG 1 cut(s) 989
SfuI TTCGAA 1 cut(s) 389
SinI GGWCC 3 cut(s) 457, 1250, 1325
SlaI CTCGAG 1 cut(s) 989
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
Sse9I AATT 6 cut(s) 158, 191, 218, 309, 779, 1432
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 9 cut(s) 137, 140, 155, 272, 349, 511, 961, 1003, 1486
SspMI CTAG 2 cut(s) 1014, 1437
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 6 cut(s) 426, 613, 631, 1117, 1290, 1363
StyI CCWWGG 4 cut(s) 453, 554, 698, 964
TaaI ACNGT 3 cut(s) 177, 625, 1278
TaiI ACGT 3 cut(s) 891, 924, 1217
TaqI TCGA 3 cut(s) 389, 990, 1127
TaqII GACCGA 1 cut(s) 474
TasI AATT 6 cut(s) 158, 191, 218, 309, 779, 1432
TatI WGTACW 1 cut(s) 222
TauI GCSGC 6 cut(s) 139, 142, 157, 352, 963, 1488
TfiI GAWTC 4 cut(s) 287, 377, 544, 694
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TscAI CASTG 5 cut(s) 129, 180, 365, 773, 1305
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 5 cut(s) 129, 180, 365, 773, 1305
Van91I CCANNNNNTGG 1 cut(s) 352
VpaK11BI GGWCC 3 cut(s) 457, 1250, 1325
XagI CCTNNNNNAGG 1 cut(s) 1362
XapI RAATTY 1 cut(s) 1432
XhoI CTCGAG 1 cut(s) 989
XspI CTAG 2 cut(s) 1014, 1437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.