pycom15g24810

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005850
Physical Location & Seq
Forward (+)
19132314 .. 19137450
5137 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g24810.2

Sequence Viewer

Length: 1245 bp
ATGGGAACTTTACTAAATAATCTCCAGCTTCAAGCTCTCTTCATTGCAGTTTGCTTATTTTCTTCCTTCTTCTGCCTCAAGATCGGAGAGACGTGTTCGAAAGACAGCGACTGCGACGCCGGTTCGAGCTGCCAAACATGCGCTGCAAATGGCAACACCCGACCCAGATGCACCCAGATTCAACCCACGAGTCCCACATCAAAGGTGAAAGGTCTACCATTCAATCGGTATTCATGGCTAACCACCCATAACGCTTTCTCTCAAGCTGGAGTCAGGTCCTCCACCGGTTCCTTAATTATTGCTCCCATGAACCAGAACGATACCGTTGCCAACCAACTCAAAAATGGCGTCCGAGGGTTTATGCTGGATATGTATGACTTCAACAATGACATCTGGTTATGTCACTCGTCTGGTGGCAAATGTTACAACTTCACTGCATTTCAACCTGCTATTAACACGCTGAGAGATATTAAAGCCTTTTTGGAAGCCAATCCCTCAGAGATTGTCACCATTTTTATTGAAGATTATGTGGAATCTCCATCGGGCCTGACAAAGGTCTTTGAGGGAGCTGGCCTAAAAAACTTTTGGTTCCCAATTGCTCGGATGCCAAAGAACGGGGAAGATTGGCCAACTGTCGATGATATGGTCCAGAATAATCAGCGGTTGGTGGTCTTCACCTCTAAGAAAGCTAAGGAGGCTTCAGAGGGAATTGCTTACGAGTGGAACTATGTCGTGGAAAATCAATATGGAAGTGGAGGAATTATACCTGGTTCATGTCCAAATCGTGCTGAATCATCTCCCATGAGCACAAAATCTAGATCATTAGTTCTCGTGAACTTCTTTCGCGATCTTCCAAATGCATCAGCATCTTGCATGGACAATTCAGCTCCCTTACTAAGCGCGTCGAAAACTTGCTATGAAGCAGCCGGGAAACGGTGGCCGAACTTCATTGCTGTTGATTATTATGAGAAAAGTGATGGTGGGGGAGCTTCAGAAGCAGTGGATGAAGCCAATGGCCATCTGACATGTGGCTGTGACAGAATCACTTACTGCAAGGTAAACGGTGGTAACGTTACATTTGGTTCATGCGACGTCCCCAAACTTGCTCCGCCGCCACCTGCCGCCGCCGCCGCCATCACATCCGCTCTACCAAGTCCCAGTAACTTCGCTTCTCAGGGACAATCAGTCCACTTGCTTTGGTTGTCTGGAACAATTTTGGCTACAATTCTCTCAATTCGGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

415

Amino Acids

44.7

Weight (kDa)

6.13

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1126
AatII GACGTC 1 cut(s) 1095
Acc36I ACCTGC 2 cut(s) 454, 1126
AccBSI CCGCTC 1 cut(s) 1145
AccI GTMKAC 1 cut(s) 214
AccII CGCG 2 cut(s) 846, 902
AciI CCGC 8 cut(s) 661, 1109, 1112, 1122, 1125, 1128, 1131, 1143
AclI AACGTT 1 cut(s) 1071
AcoI YGGCCR 3 cut(s) 626, 938, 1015
AcuI CTGAAG 2 cut(s) 684, 975
AcyI GRCGYC 3 cut(s) 117, 348, 1092
AfiI CCNNNNNNNGG 3 cut(s) 553, 614, 933
AflIII ACRYGT 2 cut(s) 92, 1025
AgeI ACCGGT 1 cut(s) 284
AgsI TTSAA 6 cut(s) 32, 182, 223, 382, 443, 521
AhdI GACNNNNNGTC 1 cut(s) 1184
AjiI CACGTC 1 cut(s) 93
AjnI CCWGG 1 cut(s) 766
AluBI AGCT 8 cut(s) 28, 35, 129, 266, 569, 689, 887, 989
AluI AGCT 8 cut(s) 28, 35, 129, 266, 569, 689, 887, 989
Alw21I GWGCWC 1 cut(s) 809
Alw26I GTCTC 1 cut(s) 83
AlwNI CAGNNNCTG 1 cut(s) 111
AoxI GGCC 5 cut(s) 544, 571, 626, 938, 1015
ApeKI GCWGC 3 cut(s) 129, 143, 923
ArsI GACNNNNNNTTYG 2 cut(s) 333, 365
AsiGI ACCGGT 1 cut(s) 284
AspLEI GCGC 2 cut(s) 143, 902
AspS9I GGNCC 3 cut(s) 276, 544, 646
AsuC2I CCSGG 1 cut(s) 928
AsuHPI GGTGA 3 cut(s) 217, 499, 667
AsuII TTCGAA 1 cut(s) 98
AvaII GGWCC 2 cut(s) 276, 646
BalI TGGCCA 2 cut(s) 628, 1017
BarI GAAGNNNNNNTAC 2 cut(s) 1153, 1185
BauI CACGAG 2 cut(s) 187, 830
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 1 cut(s) 809
BbvI GCAGC 3 cut(s) 116, 130, 935
BccI CCATC 4 cut(s) 547, 971, 1026, 1142
BcgI CGANNNNNNTGC 4 cut(s) 150, 184, 308, 342
BciT130I CCWGG 1 cut(s) 768
BcnI CCSGG 1 cut(s) 928
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 1 cut(s) 816
BfuAI ACCTGC 2 cut(s) 454, 1126
BisI GCNGC 8 cut(s) 130, 144, 924, 1112, 1122, 1125, 1128, 1131
BlsI GCNGC 8 cut(s) 131, 145, 925, 1113, 1123, 1126, 1129, 1132
Bme1390I CCNGG 2 cut(s) 768, 928
Bme18I GGWCC 2 cut(s) 276, 646
BmeRI GACNNNNNGTC 1 cut(s) 1184
BmgBI CACGTC 1 cut(s) 93
BmgT120I GGNCC 3 cut(s) 276, 544, 646
BmiI GGNNCC 2 cut(s) 289, 590
BmrFI CCNGG 2 cut(s) 768, 928
BmrI ACTGGG 1 cut(s) 1152
BmsI GCATC 4 cut(s) 158, 594, 869, 875
BmuI ACTGGG 1 cut(s) 1152
BoxI GACNNNNGTC 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 664
BpmI CTGGAG 2 cut(s) 8, 288
Bpu10I CCTNAGC 1 cut(s) 690
Bpu14I TTCGAA 1 cut(s) 98
BpuEI CTTGAG 2 cut(s) 62, 246
BpuMI CCSGG 1 cut(s) 928
BsaHI GRCGYC 3 cut(s) 117, 348, 1092
BsaJI CCNNGG 1 cut(s) 352
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 3 cut(s) 553, 614, 933
Bse118I RCCGGY 2 cut(s) 119, 284
Bse1I ACTGG 1 cut(s) 1158
Bse3DI GCAATG 2 cut(s) 42, 948
BseBI CCWGG 1 cut(s) 768
BseDI CCNNGG 1 cut(s) 352
BseGI GGATG 3 cut(s) 609, 1009, 1139
BseLI CCNNNNNNNGG 3 cut(s) 553, 614, 933
BseMI GCAATG 2 cut(s) 42, 948
BseMII CTCAG 3 cut(s) 452, 510, 1187
BseNI ACTGG 1 cut(s) 1158
BseXI GCAGC 3 cut(s) 116, 130, 935
Bsh1236I CGCG 2 cut(s) 846, 902
BshFI GGCC 5 cut(s) 546, 573, 628, 940, 1017
BshTI ACCGGT 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 809
BsiSI CCGG 3 cut(s) 120, 285, 927
BslFI GGGAC 4 cut(s) 177, 1079, 1140, 1191
BslI CCNNNNNNNGG 3 cut(s) 553, 614, 933
BsmAI GTCTC 1 cut(s) 83
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 4 cut(s) 177, 1079, 1140, 1191
BsnI GGCC 5 cut(s) 546, 573, 628, 940, 1017
Bsp119I TTCGAA 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 809
Bsp143I GATC 3 cut(s) 81, 818, 847
Bsp68I TCGCGA 1 cut(s) 846
BspACI CCGC 8 cut(s) 661, 1109, 1112, 1122, 1125, 1128, 1131, 1143
BspANI GGCC 5 cut(s) 546, 573, 628, 940, 1017
BspCNI CTCAG 3 cut(s) 453, 509, 1186
BspFNI CGCG 2 cut(s) 846, 902
BspLI GGNNCC 2 cut(s) 289, 590
BspMI ACCTGC 2 cut(s) 454, 1126
BspT104I TTCGAA 1 cut(s) 98
BsrBI CCGCTC 1 cut(s) 1145
BsrDI GCAATG 2 cut(s) 42, 948
BsrFI RCCGGY 2 cut(s) 119, 284
BsrI ACTGG 1 cut(s) 1158
BssAI RCCGGY 2 cut(s) 119, 284
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 3 cut(s) 81, 818, 847
BssNI GRCGYC 3 cut(s) 117, 348, 1092
BssSI CACGAG 2 cut(s) 187, 830
Bst2BI CACGAG 2 cut(s) 187, 830
Bst2UI CCWGG 1 cut(s) 768
Bst4CI ACNGT 4 cut(s) 325, 634, 936, 1064
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 3 cut(s) 117, 348, 1092
BstBI TTCGAA 1 cut(s) 98
BstC8I GCNNGC 1 cut(s) 571
BstDEI CTNAG 6 cut(s) 461, 496, 681, 690, 896, 1173
BstENI CCTNNNNNAGG 1 cut(s) 551
BstF5I GGATG 3 cut(s) 609, 1009, 1139
BstFNI CGCG 2 cut(s) 846, 902
BstHHI GCGC 2 cut(s) 143, 902
BstKTI GATC 3 cut(s) 84, 821, 850
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 3 cut(s) 81, 818, 847
BstMWI GCNNNNNNNGC 5 cut(s) 138, 695, 995, 1127, 1130
BstNI CCWGG 1 cut(s) 768
BstNSI RCATGY 2 cut(s) 141, 1029
BstPAI GACNNNNGTC 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 766, 926
BstUI CGCG 2 cut(s) 846, 902
BstV1I GCAGC 3 cut(s) 116, 130, 935
BstV2I GAAGAC 1 cut(s) 664
BsuRI GGCC 5 cut(s) 546, 573, 628, 940, 1017
BtrI CACGTC 1 cut(s) 93
BtsCI GGATG 3 cut(s) 609, 1009, 1139
BtsI GCAGTG 2 cut(s) 432, 1005
BtsIMutI CAGTG 2 cut(s) 432, 1005
BtuMI TCGCGA 1 cut(s) 846
BveI ACCTGC 2 cut(s) 454, 1126
Cac8I GCNNGC 1 cut(s) 571
CaiI CAGNNNCTG 1 cut(s) 111
CfoI GCGC 2 cut(s) 143, 902
Cfr10I RCCGGY 2 cut(s) 119, 284
Cfr13I GGNCC 3 cut(s) 276, 544, 646
CseI GACGC 3 cut(s) 125, 337, 891
CsiI ACCWGGT 1 cut(s) 766
CspAI ACCGGT 1 cut(s) 284
CspCI CAANNNNNGTGG 2 cut(s) 1178, 1213
CviAII CATG 8 cut(s) 138, 234, 307, 774, 802, 874, 1026, 1086
DdeI CTNAG 6 cut(s) 461, 496, 681, 690, 896, 1173
DpnI GATC 3 cut(s) 83, 820, 849
DpnII GATC 3 cut(s) 81, 818, 847
DriI GACNNNNNGTC 1 cut(s) 1184
EaeI YGGCCR 3 cut(s) 626, 938, 1015
Eam1104I CTCTTC 1 cut(s) 44
Eam1105I GACNNNNNGTC 1 cut(s) 1184
EarI CTCTTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 1098
Eco47I GGWCC 2 cut(s) 276, 646
Eco57I CTGAAG 2 cut(s) 684, 975
EcoNI CCTNNNNNAGG 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 766
EcoT22I ATGCAT 1 cut(s) 862
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 8 cut(s) 141, 237, 310, 777, 805, 877, 1029, 1089
FaqI GGGAC 4 cut(s) 177, 1079, 1140, 1191
FatI CATG 8 cut(s) 137, 233, 306, 773, 801, 873, 1025, 1085
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 8 cut(s) 130, 144, 924, 1112, 1122, 1125, 1128, 1131
FokI GGATG 3 cut(s) 616, 1016, 1126
Fsp4HI GCNGC 8 cut(s) 130, 144, 924, 1112, 1122, 1125, 1128, 1131
FspBI CTAG 1 cut(s) 816
GlaI GCGC 2 cut(s) 142, 901
GluI GCNGC 8 cut(s) 130, 144, 924, 1112, 1122, 1125, 1128, 1131
GsuI CTGGAG 2 cut(s) 8, 288
HaeIII GGCC 5 cut(s) 546, 573, 628, 940, 1017
HapII CCGG 3 cut(s) 120, 285, 927
HgaI GACGC 3 cut(s) 125, 337, 891
HhaI GCGC 2 cut(s) 143, 902
Hin1I GRCGYC 3 cut(s) 117, 348, 1092
Hin1II CATG 8 cut(s) 141, 237, 310, 777, 805, 877, 1029, 1089
Hin6I GCGC 2 cut(s) 141, 900
HinP1I GCGC 2 cut(s) 141, 900
HinfI GANTC 6 cut(s) 178, 190, 270, 533, 791, 1041
HpaII CCGG 3 cut(s) 120, 285, 927
HphI GGTGA 3 cut(s) 217, 499, 667
Hpy166II GTNNAC 4 cut(s) 215, 835, 1060, 1189
Hpy188I TCNGA 7 cut(s) 86, 353, 499, 603, 703, 994, 1023
Hpy188III TCNNGA 6 cut(s) 79, 649, 816, 832, 845, 1206
Hpy8I GTNNAC 4 cut(s) 215, 835, 1060, 1189
Hpy99I CGWCG 3 cut(s) 119, 907, 1094
HpyAV CCTTC 1 cut(s) 76
HpyCH4III ACNGT 4 cut(s) 325, 634, 936, 1064
HpyCH4IV ACGT 3 cut(s) 92, 1071, 1092
HpyCH4V TGCA 7 cut(s) 47, 146, 171, 437, 860, 873, 1053
HpyF10VI GCNNNNNNNGC 5 cut(s) 138, 695, 995, 1127, 1130
HpyF3I CTNAG 6 cut(s) 461, 496, 681, 690, 896, 1173
HpySE526I ACGT 3 cut(s) 92, 1071, 1092
Hsp92I GRCGYC 3 cut(s) 117, 348, 1092
Hsp92II CATG 8 cut(s) 141, 237, 310, 777, 805, 877, 1029, 1089
HspAI GCGC 2 cut(s) 141, 900
Kzo9I GATC 3 cut(s) 81, 818, 847
LmnI GCTCC 5 cut(s) 307, 566, 892, 986, 1111
Lsp1109I GCAGC 3 cut(s) 116, 130, 935
LweI GCATC 4 cut(s) 158, 594, 869, 875
MabI ACCWGGT 1 cut(s) 766
MaeI CTAG 1 cut(s) 816
MaeII ACGT 3 cut(s) 92, 1071, 1092
MaeIII GTNAC 7 cut(s) 401, 422, 505, 1034, 1067, 1072, 1160
MalI GATC 3 cut(s) 83, 820, 849
MbiI CCGCTC 1 cut(s) 1145
MboI GATC 3 cut(s) 81, 818, 847
MboII GAAGA 7 cut(s) 31, 54, 61, 533, 632, 664, 842
MfeI CAATTG 1 cut(s) 594
MhlI GDGCHC 1 cut(s) 809
MlsI TGGCCA 2 cut(s) 628, 1017
MluCI AATT 8 cut(s) 294, 594, 708, 759, 880, 1212, 1224, 1233
MluNI TGGCCA 2 cut(s) 628, 1017
MlyI GAGTC 2 cut(s) 199, 279
MnlI CCTC 9 cut(s) 86, 289, 347, 505, 556, 688, 688, 697, 749
Mox20I TGGCCA 2 cut(s) 628, 1017
Mph1103I ATGCAT 1 cut(s) 862
MscI TGGCCA 2 cut(s) 628, 1017
MseI TTAA 3 cut(s) 293, 453, 471
Msp20I TGGCCA 2 cut(s) 628, 1017
MspA1I CMGCKG 1 cut(s) 661
MspI CCGG 3 cut(s) 120, 285, 927
MspR9I CCNGG 2 cut(s) 768, 928
MunI CAATTG 1 cut(s) 594
MvaI CCWGG 1 cut(s) 768
MvnI CGCG 2 cut(s) 846, 902
MwoI GCNNNNNNNGC 5 cut(s) 138, 695, 995, 1127, 1130
NciI CCSGG 1 cut(s) 928
NdeII GATC 3 cut(s) 81, 818, 847
NlaIII CATG 8 cut(s) 141, 237, 310, 777, 805, 877, 1029, 1089
NlaIV GGNNCC 2 cut(s) 289, 590
NmuCI GTSAC 3 cut(s) 401, 505, 1034
NruI TCGCGA 1 cut(s) 846
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 2 cut(s) 141, 1029
NspV TTCGAA 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 1126
PciI ACATGT 1 cut(s) 1025
PcsI WCGNNNNNNNCGW 1 cut(s) 1068
PfeI GAWTC 4 cut(s) 178, 533, 791, 1041
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 8 cut(s) 131, 145, 925, 1113, 1123, 1126, 1129, 1132
PleI GAGTC 2 cut(s) 198, 278
PpsI GAGTC 2 cut(s) 198, 278
PpuMI RGGWCCY 1 cut(s) 276
PscI ACATGT 1 cut(s) 1025
PshAI GACNNNNGTC 1 cut(s) 554
Psp1406I AACGTT 1 cut(s) 1071
Psp5II RGGWCCY 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 766
PspGI CCWGG 1 cut(s) 766
PspN4I GGNNCC 2 cut(s) 289, 590
PspPI GGNCC 3 cut(s) 276, 544, 646
PspPPI RGGWCCY 1 cut(s) 276
PstNI CAGNNNCTG 1 cut(s) 111
RruI TCGCGA 1 cut(s) 846
SaqAI TTAA 3 cut(s) 293, 453, 471
SatI GCNGC 8 cut(s) 130, 144, 924, 1112, 1122, 1125, 1128, 1131
Sau3AI GATC 3 cut(s) 81, 818, 847
Sau96I GGNCC 3 cut(s) 276, 544, 646
SchI GAGTC 2 cut(s) 199, 279
ScrFI CCNGG 2 cut(s) 768, 928
SduI GDGCHC 1 cut(s) 809
SexAI ACCWGGT 1 cut(s) 766
SfaNI GCATC 4 cut(s) 158, 594, 869, 875
SfuI TTCGAA 1 cut(s) 98
SinI GGWCC 2 cut(s) 276, 646
SmlI CTYRAG 2 cut(s) 77, 261
SmoI CTYRAG 2 cut(s) 77, 261
Sse9I AATT 8 cut(s) 294, 594, 708, 759, 880, 1212, 1224, 1233
SsiI CCGC 8 cut(s) 661, 1109, 1112, 1122, 1125, 1128, 1131, 1143
SspMI CTAG 1 cut(s) 816
StyD4I CCNGG 2 cut(s) 766, 926
TaaI ACNGT 4 cut(s) 325, 634, 936, 1064
TaiI ACGT 3 cut(s) 95, 1074, 1095
TaqI TCGA 4 cut(s) 98, 125, 636, 905
TasI AATT 8 cut(s) 294, 594, 708, 759, 880, 1212, 1224, 1233
TauI GCSGC 5 cut(s) 1114, 1124, 1127, 1130, 1133
TfiI GAWTC 4 cut(s) 178, 533, 791, 1041
Tru1I TTAA 3 cut(s) 293, 453, 471
Tru9I TTAA 3 cut(s) 293, 453, 471
TscAI CASTG 2 cut(s) 439, 1005
TseFI GTSAC 3 cut(s) 401, 505, 1034
TseI GCWGC 3 cut(s) 129, 143, 923
Tsp45I GTSAC 3 cut(s) 401, 505, 1034
TspDTI ATGAA 8 cut(s) 31, 222, 323, 762, 933, 937, 1020, 1074
TspRI CASTG 2 cut(s) 439, 1005
VpaK11BI GGWCC 2 cut(s) 276, 646
XagI CCTNNNNNAGG 1 cut(s) 551
XbaI TCTAGA 1 cut(s) 815
XceI RCATGY 2 cut(s) 141, 1029
XcmI CCANNNNNNNNNTGG 2 cut(s) 341, 1025
XmiI GTMKAC 1 cut(s) 214
XspI CTAG 1 cut(s) 816
ZraI GACGTC 1 cut(s) 1093
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.