Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0007745

Physical Location & Seq

Genomic Coordinates Reverse (-)

20165746 .. 20169297

Gene Structure 3552 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g25680.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGAGCTCTCTTCTTCTTTCACACAAGTCTCCAACCTATCCGCTTACCATAACTACTCCTTCACCTCCTCCTTCTCGCAGTTCCCAACCCAAATCCGCCTCAAAACCCCCAAGTTCCCAAACCGAAGATCGCAAAATCTCCACGTCGTCGCCGTCGCCGTCGACCCACAACAGGAACTCCCCCAAAACAGCCCCCAGCGGCTCCTCAAAGAGCTCTCGGAGCGCAAAAGGGCGACTTCTCCGAAGAAGAAGGTGCCGCCGAAGCGGTTCATACTCCGGCCGCCGTTGGACGACAAGAGATTAGCGCAGAGGTTTCTGAACAGCCCACAATTGTCGCTAAAGTCGTTCCCTTTACTCAGTTCCTGCTTGCCCTCTTCGCGGCTCAACAATGCCGACAAGGCGTGGATAGAGGAGTACTTGCTGGAGGCCAAGCAAGCTCTTGGGTACCCGCTGGAGCCTTCGGATAGGTTCGAGGACGACAACCCAGCAAAGCAATTCGACACTTTGCTGTACATGGCGTTTCAGCATCCCTCCTGTGAGAGGACCAATGCCCGGCACGTCCGGTCCGCGCATTCGAGGCTGTGGTTTTTGGGGCAGTATGTGCTGGAATTGGGTTTGGCTGAGTTTTTCCTGCAGAGGTATCCAAGGGAGTCGCCGGGTCCAATGAGGGAGAGGGTGTTTGGGTTGATTGGGAAGAGGAACTTGCCCAGGTGGATTAGAGCTGCTAGCTTGCAGAATCTGGTGTTTCCTTTTGATGATATGGATAAGTTGCAGAGGAAGGACCGAGAGCCACCTGTGAAGTCTGTATTCTGGGCTTTGTTTGGGGCAATATATTTGTGTTTTGGTATGCCAGAAGTATATCGTGTTCTTTTCGAAGTATTTGGAATGGACCCAGAAGATGAGACCTGCCAGCCAAAACTACGGAGACAACTTGAAGACGTAGATTATGTTTCTGTAGAATTTGAAGGCAACAAACTCAGCTGGCAGGATGTTGCGGCTTACAGGCCCCCAGAAGATGCGCTTTTTGCACATCCAAGGCTCTTCAGGGCTTGTGTTCCACCAGGTATGCATCGGTTCAGGGGAAATGTATGGGATTATGAGAGCCGACCTAAAGTCATGCGGACATTGGGATATCCCTTAGCATTAACTGATAGAATTCCAGACATTACCGCAGCTAGGAACATCGAACTTGGACTTGGGCTACAGCTTTCTTTCTTGCATCCATCGAAGCACAAATTTGAGCATCCTCGATTTTGTTACGAGCGTTTAGAATATGTTGGCCAAAAGATCCAGGACCTTGTGATGGCAGAAAGGTTGCTGATGAAACATTTAGATGCTCCTGGAAAGTGGTTAGCAGAGAGACATCGCCGTGTTCTCATGAACAAATTCTGCGGGAGATATCTGAGAGAGAAGCGTCTCCACCAGTTTATCATCTTCTCCGACCAAGTTCAGGATGCATATGAGCACAACCGAAGATTAAGAAATCCAGCCACAACCGCTGTTCAACAAGCCATTCATGGTCTCGCATACACAATATACGGGAAGCCAGATGTAAGACGCCTCATGTTTGAGGTTTTCGATTTTGAGCAAACCCAACCTAAAGCTGTGACTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003727 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0022613 GO:0030422 GO:0031047 GO:0031050 GO:0031053 GO:0031054 GO:0032296 GO:0034470 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ribonuclease_3

Protein Analysis

539

Amino Acids

62.99

Weight (kDa)

9.54

Isoelectric Point (pI)

54.5

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48121 AT2G48121 AT4G37510
fragaria_vesca	FvH4_1g17270 FvH4_1g17270
malus_domestica	MD15G1293700.v1.1
prunus_persica	Prupe.6G223800_v2.0.a1
pyrus_communis	pycom15g25680
rosa_chinensis	RchiOBHm_Chr2g0107461
rosa_laevigata	RLG00000017580
rosa_multiflora	Rmu_sc0005152.1_g000009 Rmu_sc0025013.1_g000002 Rmu_sc0039159.1_g000001 Rmu_sc0040873.1_g000001
rosa_roxburghii	Rroxscaffold_2G00136320
rosa_rugosa	Rorug02G0143700 Rorug02G0143800
rosa_samantha	Rh2AG193900 Rh2AG299800 Rh2BG206100 Rh2CG199100 Rh2DG201100 Rh2DG323800
rosa_wichuraiana	Rw2G015360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	914
Acc65I	GGTACC	1 cut(s)	444
AccB1I	GGYRCC	2 cut(s)	253, 444
AccI	GTMKAC	1 cut(s)	162
AccII	CGCG	2 cut(s)	379, 569
AclWI	GGATC	1 cut(s)	1282
AcoI	YGGCCR	2 cut(s)	278, 1279
AcsI	RAATTY	4 cut(s)	959, 1155, 1235, 1385
AcuI	CTGAAG	1 cut(s)	1027
AcyI	GRCGYC	1 cut(s)	1558
AfaI	GTAC	3 cut(s)	416, 446, 512
AfiI	CCNNNNNNNGG	7 cut(s)	172, 378, 540, 552, 1176, 1303, 1450
AgsI	TTSAA	3 cut(s)	935, 965, 1505
AhdI	GACNNNNNGTC	1 cut(s)	1112
AjiI	CACGTC	2 cut(s)	145, 559
AjnI	CCWGG	4 cut(s)	707, 1060, 1290, 1339
AjuI	GAANNNNNNNTTGG	2 cut(s)	1179, 1211
AluBI	AGCT	9 cut(s)	7, 214, 437, 722, 729, 981, 1175, 1207, 1604
AluI	AGCT	9 cut(s)	7, 214, 437, 722, 729, 981, 1175, 1207, 1604
Alw21I	GWGCWC	3 cut(s)	9, 216, 1467
Alw26I	GTCTC	6 cut(s)	34, 896, 919, 1354, 1421, 1526
AlwI	GGATC	1 cut(s)	1282
AlwNI	CAGNNNCTG	2 cut(s)	363, 739
AoxI	GGCC	4 cut(s)	278, 426, 1004, 1279
ApeKI	GCWGC	2 cut(s)	722, 1172
ApoI	RAATTY	4 cut(s)	959, 1155, 1235, 1385
Asp700I	GAANNNNTTC	2 cut(s)	266, 1385
Asp718I	GGTACC	1 cut(s)	444
AspLEI	GCGC	4 cut(s)	225, 307, 571, 1021
AspS9I	GGNCC	7 cut(s)	543, 564, 659, 781, 889, 1005, 1294
AsuC2I	CCSGG	2 cut(s)	553, 657
AsuHPI	GGTGA	1 cut(s)	55
AsuII	TTCGAA	1 cut(s)	873
AsuNHI	GCTAGC	1 cut(s)	725
AvaII	GGWCC	6 cut(s)	543, 564, 659, 781, 889, 1294
BalI	TGGCCA	1 cut(s)	1281
BanI	GGYRCC	2 cut(s)	253, 444
BanII	GRGCYC	2 cut(s)	9, 216
BbsI	GAAGAC	1 cut(s)	942
Bbv12I	GWGCWC	3 cut(s)	9, 216, 1467
BbvI	GCAGC	2 cut(s)	709, 1184
BccI	CCATC	2 cut(s)	1231, 1297
BceAI	ACGGC	4 cut(s)	137, 143, 268, 1353
BciT130I	CCWGG	4 cut(s)	709, 1062, 1292, 1341
BciVI	GTATCC	1 cut(s)	651
BcnI	CCSGG	2 cut(s)	553, 657
BcoDI	GTCTC	6 cut(s)	34, 896, 919, 1354, 1421, 1526
BfaI	CTAG	2 cut(s)	726, 1176
BfmI	CTRYAG	3 cut(s)	632, 954, 1202
BfuAI	ACCTGC	1 cut(s)	914
BfuI	GTATCC	1 cut(s)	651
BglI	GCCNNNNNGGC	1 cut(s)	398
BisI	GCNGC	7 cut(s)	200, 257, 281, 380, 723, 996, 1173
BlsI	GCNGC	7 cut(s)	201, 258, 282, 381, 724, 997, 1174
BmcAI	AGTACT	1 cut(s)	416
Bme1390I	CCNGG	6 cut(s)	553, 657, 709, 1062, 1292, 1341
Bme18I	GGWCC	6 cut(s)	543, 564, 659, 781, 889, 1294
BmeRI	GACNNNNNGTC	1 cut(s)	1112
BmgBI	CACGTC	2 cut(s)	145, 559
BmgT120I	GGNCC	7 cut(s)	543, 564, 659, 781, 889, 1005, 1294
BmiI	GGNNCC	7 cut(s)	203, 255, 446, 456, 660, 891, 1007
BmrFI	CCNGG	6 cut(s)	553, 657, 709, 1062, 1292, 1341
BmsI	GCATC	7 cut(s)	535, 1006, 1078, 1228, 1252, 1324, 1444
BmtI	GCTAGC	1 cut(s)	729
BpiI	GAAGAC	1 cut(s)	942
BpmI	CTGGAG	2 cut(s)	443, 473
Bpu10I	CCTNAGC	1 cut(s)	1138
Bpu14I	TTCGAA	1 cut(s)	873
BpuMI	CCSGG	2 cut(s)	553, 657
BsaHI	GRCGYC	1 cut(s)	1558
BsaI	GGTCTC	2 cut(s)	896, 1526
BsaJI	CCNNGG	3 cut(s)	644, 707, 1034
BsaWI	WCCGGW	1 cut(s)	561
BsaXI	ACNNNNNCTCC	4 cut(s)	162, 192, 662, 692
Bsc4I	CCNNNNNNNGG	7 cut(s)	172, 378, 540, 552, 1176, 1303, 1450
Bse1I	ACTGG	1 cut(s)	1423
BseBI	CCWGG	4 cut(s)	709, 1062, 1292, 1341
BseDI	CCNNGG	3 cut(s)	644, 707, 1034
BseGI	GGATG	6 cut(s)	526, 994, 1030, 1219, 1243, 1459
BseLI	CCNNNNNNNGG	7 cut(s)	172, 378, 540, 552, 1176, 1303, 1450
BseMII	CTCAG	4 cut(s)	370, 612, 991, 1394
BseNI	ACTGG	1 cut(s)	1423
BseRI	GAGGAG	3 cut(s)	58, 194, 425
BseX3I	CGGCCG	1 cut(s)	278
BseXI	GCAGC	2 cut(s)	709, 1184
BseYI	CCCAGC	2 cut(s)	195, 484
Bsh1236I	CGCG	2 cut(s)	379, 569
Bsh1285I	CGRYCG	1 cut(s)	281
BshFI	GGCC	4 cut(s)	280, 428, 1006, 1281
BshNI	GGYRCC	2 cut(s)	253, 444
BsiEI	CGRYCG	1 cut(s)	281
BsiHKAI	GWGCWC	3 cut(s)	9, 216, 1467
BsiSI	CCGG	4 cut(s)	277, 553, 562, 656
BslI	CCNNNNNNNGG	7 cut(s)	172, 378, 540, 552, 1176, 1303, 1450
BsmAI	GTCTC	6 cut(s)	34, 896, 919, 1354, 1421, 1526
BsmBI	CGTCTC	1 cut(s)	1421
BsmI	GAATGC	1 cut(s)	571
BsnI	GGCC	4 cut(s)	280, 428, 1006, 1281
Bso31I	GGTCTC	2 cut(s)	896, 1526
Bsp119I	TTCGAA	1 cut(s)	873
Bsp1286I	GDGCHC	3 cut(s)	9, 216, 1467
Bsp1407I	TGTACA	1 cut(s)	510
Bsp143I	GATC	2 cut(s)	128, 1287
BspANI	GGCC	4 cut(s)	280, 428, 1006, 1281
BspCNI	CTCAG	4 cut(s)	369, 613, 990, 1395
BspFNI	CGCG	2 cut(s)	379, 569
BspHI	TCATGA	1 cut(s)	1377
BspLI	GGNNCC	7 cut(s)	203, 255, 446, 456, 660, 891, 1007
BspMAI	CTGCAG	1 cut(s)	636
BspMI	ACCTGC	1 cut(s)	914
BspOI	GCTAGC	1 cut(s)	729
BspPI	GGATC	1 cut(s)	1282
BspQI	GCTCTTC	1 cut(s)	1046
BspT104I	TTCGAA	1 cut(s)	873
BspT107I	GGYRCC	2 cut(s)	253, 444
BspTNI	GGTCTC	2 cut(s)	896, 1526
BsrGI	TGTACA	1 cut(s)	510
BsrI	ACTGG	1 cut(s)	1423
BssECI	CCNNGG	3 cut(s)	644, 707, 1034
BssMI	GATC	2 cut(s)	128, 1287
BssNI	GRCGYC	1 cut(s)	1558
BssT1I	CCWWGG	2 cut(s)	644, 1034
Bst2UI	CCWGG	4 cut(s)	709, 1062, 1292, 1341
Bst4CI	ACNGT	1 cut(s)	1612
Bst6I	CTCTTC	4 cut(s)	16, 379, 689, 1046
BstACI	GRCGYC	1 cut(s)	1558
BstAPI	GCANNNNNTGC	1 cut(s)	601
BstAUI	TGTACA	1 cut(s)	510
BstBI	TTCGAA	1 cut(s)	873
BstC8I	GCNNGC	6 cut(s)	368, 435, 727, 731, 911, 983
BstDEI	CTNAG	5 cut(s)	356, 621, 977, 1138, 1403
BstENI	CCTNNNNNAGG	1 cut(s)	538
BstF5I	GGATG	6 cut(s)	526, 994, 1030, 1219, 1243, 1459
BstFNI	CGCG	2 cut(s)	379, 569
BstHHI	GCGC	4 cut(s)	225, 307, 571, 1021
BstKTI	GATC	2 cut(s)	131, 1290
BstMAI	GTCTC	6 cut(s)	34, 896, 919, 1354, 1421, 1526
BstMBI	GATC	2 cut(s)	128, 1287
BstMCI	CGRYCG	1 cut(s)	281
BstMWI	GCNNNNNNNGC	9 cut(s)	220, 262, 376, 398, 434, 577, 601, 1025, 1496
BstNI	CCWGG	4 cut(s)	709, 1062, 1292, 1341
BstSCI	CCNGG	6 cut(s)	551, 655, 707, 1060, 1290, 1339
BstSFI	CTRYAG	3 cut(s)	632, 954, 1202
BstUI	CGCG	2 cut(s)	379, 569
BstV1I	GCAGC	2 cut(s)	709, 1184
BstV2I	GAAGAC	1 cut(s)	942
BstX2I	RGATCY	1 cut(s)	1287
BstYI	RGATCY	1 cut(s)	1287
BstZI	CGGCCG	1 cut(s)	278
BsuI	GTATCC	1 cut(s)	651
BsuRI	GGCC	4 cut(s)	280, 428, 1006, 1281
BtgZI	GCGATG	1 cut(s)	1349
BtrI	CACGTC	2 cut(s)	145, 559
BtsCI	GGATG	6 cut(s)	526, 994, 1030, 1219, 1243, 1459
BveI	ACCTGC	1 cut(s)	914
Cac8I	GCNNGC	6 cut(s)	368, 435, 727, 731, 911, 983
CaiI	CAGNNNCTG	2 cut(s)	363, 739
CciI	TCATGA	1 cut(s)	1377
CfoI	GCGC	4 cut(s)	225, 307, 571, 1021
Cfr13I	GGNCC	7 cut(s)	543, 564, 659, 781, 889, 1005, 1294
CpoI	CGGWCCG	1 cut(s)	564
CseI	GACGC	2 cut(s)	1403, 1566
CsiI	ACCWGGT	1 cut(s)	1060
Csp6I	GTAC	3 cut(s)	415, 445, 511
CspI	CGGWCCG	1 cut(s)	564
CviAII	CATG	5 cut(s)	514, 1117, 1378, 1517, 1564
CviQI	GTAC	3 cut(s)	415, 445, 511
DdeI	CTNAG	5 cut(s)	356, 621, 977, 1138, 1403
DpnI	GATC	2 cut(s)	130, 1289
DpnII	GATC	2 cut(s)	128, 1287
DriI	GACNNNNNGTC	1 cut(s)	1112
EaeI	YGGCCR	2 cut(s)	278, 1279
EagI	CGGCCG	1 cut(s)	278
Eam1104I	CTCTTC	4 cut(s)	16, 379, 689, 1046
Eam1105I	GACNNNNNGTC	1 cut(s)	1112
EarI	CTCTTC	4 cut(s)	16, 379, 689, 1046
EciI	GGCGGA	1 cut(s)	86
Ecl136II	GAGCTC	2 cut(s)	7, 214
EclXI	CGGCCG	1 cut(s)	278
Eco130I	CCWWGG	2 cut(s)	644, 1034
Eco24I	GRGCYC	2 cut(s)	9, 216
Eco31I	GGTCTC	2 cut(s)	896, 1526
Eco32I	GATATC	2 cut(s)	1133, 1400
Eco47I	GGWCC	6 cut(s)	543, 564, 659, 781, 889, 1294
Eco52I	CGGCCG	1 cut(s)	278
Eco53kI	GAGCTC	2 cut(s)	7, 214
Eco57I	CTGAAG	1 cut(s)	1027
EcoICRI	GAGCTC	2 cut(s)	7, 214
EcoNI	CCTNNNNNAGG	1 cut(s)	538
EcoO109I	RGGNCCY	2 cut(s)	1005, 1294
EcoRI	GAATTC	1 cut(s)	1155
EcoRII	CCWGG	4 cut(s)	707, 1060, 1290, 1339
EcoRV	GATATC	2 cut(s)	1133, 1400
EcoT14I	CCWWGG	2 cut(s)	644, 1034
EcoT22I	ATGCAT	2 cut(s)	1071, 1459
EcoT38I	GRGCYC	2 cut(s)	9, 216
ErhI	CCWWGG	2 cut(s)	644, 1034
Esp3I	CGTCTC	1 cut(s)	1421
FaeI	CATG	5 cut(s)	517, 1120, 1381, 1520, 1567
FalI	AAGNNNNNCTT	6 cut(s)	220, 252, 686, 718, 1005, 1037
FatI	CATG	5 cut(s)	513, 1116, 1377, 1516, 1563
FauI	CCCGC	2 cut(s)	456, 1385
FauNDI	CATATG	1 cut(s)	1459
FblI	GTMKAC	1 cut(s)	162
Fnu4HI	GCNGC	7 cut(s)	200, 257, 281, 380, 723, 996, 1173
FokI	GGATG	6 cut(s)	513, 1001, 1017, 1206, 1230, 1466
FriOI	GRGCYC	2 cut(s)	9, 216
Fsp4HI	GCNGC	7 cut(s)	200, 257, 281, 380, 723, 996, 1173
FspBI	CTAG	2 cut(s)	726, 1176
GlaI	GCGC	4 cut(s)	224, 306, 570, 1020
GluI	GCNGC	7 cut(s)	200, 257, 281, 380, 723, 996, 1173
GsaI	CCCAGC	2 cut(s)	199, 488
GsuI	CTGGAG	2 cut(s)	443, 473
HaeIII	GGCC	4 cut(s)	280, 428, 1006, 1281
HapII	CCGG	4 cut(s)	277, 553, 562, 656
HgaI	GACGC	2 cut(s)	1403, 1566
HhaI	GCGC	4 cut(s)	225, 307, 571, 1021
Hin1I	GRCGYC	1 cut(s)	1558
Hin1II	CATG	5 cut(s)	517, 1120, 1381, 1520, 1567
Hin6I	GCGC	4 cut(s)	223, 305, 569, 1019
HinP1I	GCGC	4 cut(s)	223, 305, 569, 1019
HincII	GTYRAC	1 cut(s)	163
HindII	GTYRAC	1 cut(s)	163
HinfI	GANTC	2 cut(s)	650, 736
HpaII	CCGG	4 cut(s)	277, 553, 562, 656
HphI	GGTGA	1 cut(s)	55
Hpy166II	GTNNAC	1 cut(s)	163
Hpy188I	TCNGA	6 cut(s)	220, 243, 318, 463, 1404, 1441
Hpy188III	TCNNGA	3 cut(s)	1160, 1378, 1451
Hpy8I	GTNNAC	1 cut(s)	163
Hpy99I	CGWCG	4 cut(s)	149, 152, 158, 164
HpyAV	CCTTC	6 cut(s)	70, 82, 244, 468, 772, 959
HpyCH4III	ACNGT	1 cut(s)	1612
HpyCH4IV	ACGT	3 cut(s)	144, 558, 939
HpyCH4V	TGCA	7 cut(s)	634, 733, 772, 1028, 1069, 1219, 1457
HpyF10VI	GCNNNNNNNGC	9 cut(s)	220, 262, 376, 398, 434, 577, 601, 1025, 1496
HpyF3I	CTNAG	5 cut(s)	356, 621, 977, 1138, 1403
HpySE526I	ACGT	3 cut(s)	144, 558, 939
Hsp92I	GRCGYC	1 cut(s)	1558
Hsp92II	CATG	5 cut(s)	517, 1120, 1381, 1520, 1567
HspAI	GCGC	4 cut(s)	223, 305, 569, 1019
KpnI	GGTACC	1 cut(s)	448
Kzo9I	GATC	2 cut(s)	128, 1287
LguI	GCTCTTC	1 cut(s)	1046
LmnI	GCTCC	5 cut(s)	4, 207, 220, 454, 1342
Lsp1109I	GCAGC	2 cut(s)	709, 1184
LweI	GCATC	7 cut(s)	535, 1006, 1078, 1228, 1252, 1324, 1444
MabI	ACCWGGT	1 cut(s)	1060
MaeI	CTAG	2 cut(s)	726, 1176
MaeII	ACGT	3 cut(s)	144, 558, 939
MaeIII	GTNAC	2 cut(s)	1256, 1606
MalI	GATC	2 cut(s)	130, 1289
MboI	GATC	2 cut(s)	128, 1287
MfeI	CAATTG	1 cut(s)	329
MflI	RGATCY	1 cut(s)	1287
MhlI	GDGCHC	3 cut(s)	9, 216, 1467
MlsI	TGGCCA	1 cut(s)	1281
MluCI	AATT	7 cut(s)	329, 494, 608, 959, 1155, 1235, 1385
MluNI	TGGCCA	1 cut(s)	1281
MlyI	GAGTC	1 cut(s)	659
MmeI	TCCRAC	3 cut(s)	57, 267, 1464
Mox20I	TGGCCA	1 cut(s)	1281
Mph1103I	ATGCAT	2 cut(s)	1071, 1459
MroXI	GAANNNNTTC	2 cut(s)	266, 1385
MscI	TGGCCA	1 cut(s)	1281
MseI	TTAA	2 cut(s)	1145, 1478
MslI	CAYNNNNRTG	2 cut(s)	1332, 1368
Msp20I	TGGCCA	1 cut(s)	1281
MspA1I	CMGCKG	4 cut(s)	199, 451, 981, 1499
MspI	CCGG	4 cut(s)	277, 553, 562, 656
MspR9I	CCNGG	6 cut(s)	553, 657, 709, 1062, 1292, 1341
MunI	CAATTG	1 cut(s)	329
Mva1269I	GAATGC	1 cut(s)	571
MvaI	CCWGG	4 cut(s)	709, 1062, 1292, 1341
MvnI	CGCG	2 cut(s)	379, 569
MwoI	GCNNNNNNNGC	9 cut(s)	220, 262, 376, 398, 434, 577, 601, 1025, 1496
NciI	CCSGG	2 cut(s)	553, 657
NdeI	CATATG	1 cut(s)	1459
NdeII	GATC	2 cut(s)	128, 1287
NheI	GCTAGC	1 cut(s)	725
NlaIII	CATG	5 cut(s)	517, 1120, 1381, 1520, 1567
NlaIV	GGNNCC	7 cut(s)	203, 255, 446, 456, 660, 891, 1007
NmuCI	GTSAC	1 cut(s)	1606
NsiI	ATGCAT	2 cut(s)	1071, 1459
NspV	TTCGAA	1 cut(s)	873
PagI	TCATGA	1 cut(s)	1377
PciSI	GCTCTTC	1 cut(s)	1046
PcsI	WCGNNNNNNNCGW	3 cut(s)	150, 156, 341
PctI	GAATGC	1 cut(s)	571
PdmI	GAANNNNTTC	2 cut(s)	266, 1385
PfeI	GAWTC	1 cut(s)	736
PfoI	TCCNGGA	2 cut(s)	1290, 1339
PkrI	GCNGC	7 cut(s)	201, 258, 282, 381, 724, 997, 1174
PleI	GAGTC	1 cut(s)	658
PpsI	GAGTC	1 cut(s)	658
PpuMI	RGGWCCY	1 cut(s)	1294
Psp124BI	GAGCTC	2 cut(s)	9, 216
Psp5II	RGGWCCY	1 cut(s)	1294
Psp6I	CCWGG	4 cut(s)	707, 1060, 1290, 1339
PspFI	CCCAGC	2 cut(s)	195, 484
PspGI	CCWGG	4 cut(s)	707, 1060, 1290, 1339
PspN4I	GGNNCC	7 cut(s)	203, 255, 446, 456, 660, 891, 1007
PspPI	GGNCC	7 cut(s)	543, 564, 659, 781, 889, 1005, 1294
PspPPI	RGGWCCY	1 cut(s)	1294
PsrI	GAACNNNNNNTAC	2 cut(s)	849, 881
PstI	CTGCAG	1 cut(s)	636
PstNI	CAGNNNCTG	2 cut(s)	363, 739
PsuI	RGATCY	1 cut(s)	1287
PvuII	CAGCTG	1 cut(s)	981
RsaI	GTAC	3 cut(s)	416, 446, 512
RsaNI	GTAC	3 cut(s)	415, 445, 511
RseI	CAYNNNNRTG	2 cut(s)	1332, 1368
Rsr2I	CGGWCCG	1 cut(s)	564
RsrII	CGGWCCG	1 cut(s)	564
SacI	GAGCTC	2 cut(s)	9, 216
SalI	GTCGAC	1 cut(s)	161
SapI	GCTCTTC	1 cut(s)	1046
SaqAI	TTAA	2 cut(s)	1145, 1478
SatI	GCNGC	7 cut(s)	200, 257, 281, 380, 723, 996, 1173
Sau3AI	GATC	2 cut(s)	128, 1287
Sau96I	GGNCC	7 cut(s)	543, 564, 659, 781, 889, 1005, 1294
ScaI	AGTACT	1 cut(s)	416
SchI	GAGTC	1 cut(s)	659
ScrFI	CCNGG	6 cut(s)	553, 657, 709, 1062, 1292, 1341
SduI	GDGCHC	3 cut(s)	9, 216, 1467
SexAI	ACCWGGT	1 cut(s)	1060
SfaNI	GCATC	7 cut(s)	535, 1006, 1078, 1228, 1252, 1324, 1444
SfcI	CTRYAG	3 cut(s)	632, 954, 1202
SfuI	TTCGAA	1 cut(s)	873
SinI	GGWCC	6 cut(s)	543, 564, 659, 781, 889, 1294
SmiMI	CAYNNNNRTG	2 cut(s)	1332, 1368
Sse9I	AATT	7 cut(s)	329, 494, 608, 959, 1155, 1235, 1385
SspMI	CTAG	2 cut(s)	726, 1176
SstI	GAGCTC	2 cut(s)	9, 216
StyD4I	CCNGG	6 cut(s)	551, 655, 707, 1060, 1290, 1339
StyI	CCWWGG	2 cut(s)	644, 1034
TaaI	ACNGT	1 cut(s)	1612
TaiI	ACGT	3 cut(s)	147, 561, 942
TaqI	TCGA	9 cut(s)	162, 471, 498, 575, 873, 1185, 1226, 1249, 1578
TaqII	GACCGA	1 cut(s)	798
TasI	AATT	7 cut(s)	329, 494, 608, 959, 1155, 1235, 1385
TatI	WGTACW	2 cut(s)	414, 510
TauI	GCSGC	5 cut(s)	202, 259, 283, 382, 998
TfiI	GAWTC	1 cut(s)	736
Tru1I	TTAA	2 cut(s)	1145, 1478
Tru9I	TTAA	2 cut(s)	1145, 1478
TseFI	GTSAC	1 cut(s)	1606
TseI	GCWGC	2 cut(s)	722, 1172
Tsp45I	GTSAC	1 cut(s)	1606
TspDTI	ATGAA	4 cut(s)	259, 1337, 1394, 1505
TspGWI	ACGGA	1 cut(s)	937
VpaK11BI	GGWCC	6 cut(s)	543, 564, 659, 781, 889, 1294
XagI	CCTNNNNNAGG	1 cut(s)	538
XapI	RAATTY	4 cut(s)	959, 1155, 1235, 1385
XmiI	GTMKAC	1 cut(s)	162
XmnI	GAANNNNTTC	2 cut(s)	266, 1385
XspI	CTAG	2 cut(s)	726, 1176
ZrmI	AGTACT	1 cut(s)	416
Zsp2I	ATGCAT	2 cut(s)	1071, 1459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.