pycom15g27850

14-3-3-like protein GF14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004574
Physical Location & Seq
Forward (+)
23375922 .. 23376891
970 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGGCTTCCGCCGTCCCTGAAAATCTCAGCAGAGAACAGTACGTGTACCTGGCGAAGCTCGCCGAGCAGGCCGAGCGCTACGAGGAGATGGTGAGCTTCATGGAGAAGCTTGTCGTCGGCTCCACCGCCGCCGGGACCGAGCTGACGGTGGAGGAGCGCAACCTGCTCAGCGTCGCCTACAAGAACGTCATCGGCTCGCTCCGCGCCGCCTGGCGCATAATCTCGTCCATCGAGCAGAAGGAGGAAGGCCGCCACAACGAGGAGCACGTGGCGCTCGTCAAGCAGTACAGATCCAAGGTGGAGACCGAACTCTCCGCCATCTGCGCCGGAATCCTGGAGCTCCTGCAGTCGCACCTTGTTCCTTCGGCTACCACCGGAGAGTCCAAGGTCTTCTACTTGAAGATGAAGGGAGATTACCATCGGTACCTCGCCGAGTTCAAAAATGGCGACGAGAGAAAGACCGCCGCCGAGGACACCATGGTTGCCTACAAGGCTGCTCAGGATATTGCGCTGGCTGATTTGGCGCCTACGCATCCAATAAGGTTGGGGCTGGCGCTCAACTTCTCGGTGTTTTACTACGAGATCCTTAACTCCTCGGAGAAAGCTTGTAGCATGGCTAAACAGGTTCGGACTTATGAAATTGTTCAAGTTATTGGTTTTAAAACTTGTAATTCTTTGTTTGTTTGGATTTTTCTAATATTTGATCATGTGCTTAAAAAAGGGAAACACTGGAGGATGTTGAATTCGAGAATGCGAGTGAAAACGTCGATTGCGTTTACCTGTCTAGTTATTTGTTTGTTTGGAATTAAGGCACCTTCATTTGCATGGAGTCGAATTAGTAGTGGAAGATGCAGGCTACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

288

Amino Acids

32.51

Weight (kDa)

8.9

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 210 3e-81 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 171
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 3 cut(s) 423, 523, 811
AccII CGCG 1 cut(s) 204
AciI CCGC 9 cut(s) 9, 126, 129, 202, 207, 250, 315, 462, 465
AclWI GGATC 2 cut(s) 285, 577
AcsI RAATTY 1 cut(s) 742
AcvI CACGTG 1 cut(s) 268
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 4 cut(s) 41, 47, 287, 425
AfeI AGCGCT 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 132, 259
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 4 cut(s) 400, 439, 647, 742
AjnI CCWGG 3 cut(s) 48, 209, 333
AluBI AGCT 6 cut(s) 58, 96, 109, 142, 340, 605
AluI AGCT 6 cut(s) 58, 96, 109, 142, 340, 605
Alw21I GWGCWC 2 cut(s) 267, 342
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 2 cut(s) 285, 577
Aor51HI AGCGCT 1 cut(s) 77
AoxI GGCC 2 cut(s) 69, 247
ApeKI GCWGC 1 cut(s) 494
ApoI RAATTY 1 cut(s) 742
Asp700I GAANNNNTTC 1 cut(s) 642
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 9 cut(s) 78, 159, 206, 216, 274, 326, 511, 526, 556
AspS9I GGNCC 1 cut(s) 135
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 135
BanI GGYRCC 3 cut(s) 423, 523, 811
BanII GRGCYC 1 cut(s) 342
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BbrPI CACGTG 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 382
Bbv12I GWGCWC 2 cut(s) 267, 342
BbvI GCAGC 1 cut(s) 481
BccI CCATC 4 cut(s) 82, 236, 326, 426
BciT130I CCWGG 3 cut(s) 50, 211, 335
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 1 cut(s) 785
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 4 cut(s) 79, 275, 527, 557
BfuAI ACCTGC 1 cut(s) 171
BglI GCCNNNNNGGC 2 cut(s) 68, 491
BisI GCNGC 5 cut(s) 129, 207, 250, 465, 495
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 5 cut(s) 130, 208, 251, 466, 496
Bme1390I CCNGG 4 cut(s) 50, 133, 211, 335
Bme18I GGWCC 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 5 cut(s) 121, 136, 425, 525, 813
BmrFI CCNGG 4 cut(s) 50, 133, 211, 335
BmsI GCATC 2 cut(s) 541, 839
BpiI GAAGAC 1 cut(s) 382
BpmI CTGGAG 2 cut(s) 356, 751
Bpu10I CCTNAGC 1 cut(s) 498
Bpu1102I GCTNAGC 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 133
BsaAI YACGTR 2 cut(s) 43, 268
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 524
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 259
Bse1I ACTGG 1 cut(s) 734
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 3 cut(s) 50, 211, 335
BseDI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BseGI GGATG 2 cut(s) 532, 741
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 132, 259
BseMII CTCAG 3 cut(s) 40, 181, 512
BseNI ACTGG 1 cut(s) 734
BseRI GAGGAG 4 cut(s) 98, 167, 275, 583
BseXI GCAGC 1 cut(s) 481
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 71, 249
BshNI GGYRCC 3 cut(s) 423, 523, 811
BsiHKAI GWGCWC 2 cut(s) 267, 342
BsiSI CCGG 3 cut(s) 132, 327, 375
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 2 cut(s) 132, 259
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 148
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 2 cut(s) 71, 249
Bso31I GGTCTC 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 267, 342
Bsp143I GATC 3 cut(s) 290, 582, 703
Bsp1720I GCTNAGC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 9 cut(s) 9, 126, 129, 202, 207, 250, 315, 462, 465
BspANI GGCC 2 cut(s) 71, 249
BspCNI CTCAG 3 cut(s) 39, 180, 511
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 5 cut(s) 121, 136, 425, 525, 813
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 171
BspPI GGATC 2 cut(s) 285, 577
BspT107I GGYRCC 3 cut(s) 423, 523, 811
BspTNI GGTCTC 1 cut(s) 296
BsrI ACTGG 1 cut(s) 734
BssECI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BssMI GATC 3 cut(s) 290, 582, 703
BssNI GRCGYC 1 cut(s) 524
BssT1I CCWWGG 3 cut(s) 294, 384, 477
Bst2UI CCWGG 3 cut(s) 50, 211, 335
Bst4CI ACNGT 2 cut(s) 39, 148
BstACI GRCGYC 1 cut(s) 524
BstBAI YACGTR 2 cut(s) 43, 268
BstC8I GCNNGC 6 cut(s) 60, 69, 197, 513, 552, 854
BstDEI CTNAG 3 cut(s) 26, 167, 498
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 2 cut(s) 532, 741
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 4 cut(s) 79, 275, 527, 557
BstHHI GCGC 9 cut(s) 78, 159, 206, 216, 274, 326, 511, 526, 556
BstKTI GATC 3 cut(s) 293, 585, 706
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 3 cut(s) 290, 582, 703
BstNI CCWGG 3 cut(s) 50, 211, 335
BstSCI CCNGG 4 cut(s) 48, 131, 209, 333
BstSFI CTRYAG 1 cut(s) 344
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 481
BstV2I GAAGAC 1 cut(s) 382
BstX2I RGATCY 2 cut(s) 290, 582
BstYI RGATCY 2 cut(s) 290, 582
BsuRI GGCC 2 cut(s) 71, 249
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 532, 741
BtsIMutI CAGTG 1 cut(s) 727
BveI ACCTGC 1 cut(s) 171
Cac8I GCNNGC 6 cut(s) 60, 69, 197, 513, 552, 854
CfoI GCGC 9 cut(s) 78, 159, 206, 216, 274, 326, 511, 526, 556
Cfr13I GGNCC 1 cut(s) 135
CseI GACGC 1 cut(s) 160
Csp6I GTAC 4 cut(s) 40, 46, 286, 424
CviAII CATG 5 cut(s) 100, 478, 613, 707, 825
CviQI GTAC 4 cut(s) 40, 46, 286, 424
DdeI CTNAG 3 cut(s) 26, 167, 498
DinI GGCGCC 1 cut(s) 525
DpnI GATC 3 cut(s) 292, 584, 705
DpnII GATC 3 cut(s) 290, 582, 703
DraI TTTAAA 1 cut(s) 661
EciI GGCGGA 1 cut(s) 304
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 3 cut(s) 294, 384, 477
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 135
Eco47III AGCGCT 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 340
Eco72I CACGTG 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 742
EcoRII CCWGG 3 cut(s) 48, 209, 333
EcoT14I CCWWGG 3 cut(s) 294, 384, 477
EcoT38I GRGCYC 1 cut(s) 342
EgeI GGCGCC 1 cut(s) 525
EheI GGCGCC 1 cut(s) 525
ErhI CCWWGG 3 cut(s) 294, 384, 477
FaeI CATG 5 cut(s) 103, 481, 616, 710, 828
FaiI YATR 7 cut(s) 101, 218, 479, 614, 636, 708, 826
FaqI GGGAC 1 cut(s) 148
FatI CATG 5 cut(s) 99, 477, 612, 706, 824
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 5 cut(s) 129, 207, 250, 465, 495
FokI GGATG 2 cut(s) 519, 748
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 5 cut(s) 129, 207, 250, 465, 495
FspBI CTAG 1 cut(s) 785
GlaI GCGC 9 cut(s) 77, 158, 205, 215, 273, 325, 510, 525, 555
GluI GCNGC 5 cut(s) 129, 207, 250, 465, 495
GsuI CTGGAG 2 cut(s) 356, 751
HaeII RGCGCY 4 cut(s) 79, 275, 527, 557
HaeIII GGCC 2 cut(s) 71, 249
HapII CCGG 3 cut(s) 132, 327, 375
HgaI GACGC 1 cut(s) 160
HhaI GCGC 9 cut(s) 78, 159, 206, 216, 274, 326, 511, 526, 556
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 5 cut(s) 103, 481, 616, 710, 828
Hin6I GCGC 9 cut(s) 76, 157, 204, 214, 272, 324, 509, 524, 554
HinP1I GCGC 9 cut(s) 76, 157, 204, 214, 272, 324, 509, 524, 554
HindIII AAGCTT 2 cut(s) 107, 603
HinfI GANTC 3 cut(s) 330, 380, 829
HpaII CCGG 3 cut(s) 132, 327, 375
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 46, 777
Hpy188I TCNGA 2 cut(s) 598, 630
Hpy188III TCNNGA 2 cut(s) 500, 747
Hpy8I GTNNAC 2 cut(s) 46, 777
Hpy99I CGWCG 4 cut(s) 119, 176, 452, 769
HpyAV CCTTC 5 cut(s) 232, 239, 372, 400, 825
HpyCH4III ACNGT 2 cut(s) 39, 148
HpyCH4IV ACGT 4 cut(s) 42, 186, 267, 764
HpyCH4V TGCA 3 cut(s) 346, 824, 852
HpyF3I CTNAG 3 cut(s) 26, 167, 498
HpySE526I ACGT 4 cut(s) 42, 186, 267, 764
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 5 cut(s) 103, 481, 616, 710, 828
HspAI GCGC 9 cut(s) 76, 157, 204, 214, 272, 324, 509, 524, 554
KasI GGCGCC 1 cut(s) 523
KpnI GGTACC 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 3 cut(s) 290, 582, 703
LmnI GCTCC 6 cut(s) 125, 154, 204, 262, 337, 345
Lsp1109I GCAGC 1 cut(s) 481
LweI GCATC 2 cut(s) 541, 839
MaeI CTAG 1 cut(s) 785
MaeII ACGT 4 cut(s) 42, 186, 267, 764
MalI GATC 3 cut(s) 292, 584, 705
MboI GATC 3 cut(s) 290, 582, 703
MboII GAAGA 3 cut(s) 382, 412, 858
MflI RGATCY 2 cut(s) 290, 582
MhlI GDGCHC 2 cut(s) 267, 342
MluCI AATT 5 cut(s) 639, 670, 742, 804, 834
Mly113I GGCGCC 1 cut(s) 524
MlyI GAGTC 2 cut(s) 389, 838
MnlI CCTC 8 cut(s) 76, 145, 235, 253, 437, 463, 604, 726
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 4 cut(s) 588, 660, 714, 807
MslI CAYNNNNRTG 1 cut(s) 823
MspI CCGG 3 cut(s) 132, 327, 375
MspR9I CCNGG 4 cut(s) 50, 133, 211, 335
Mva1269I GAATGC 1 cut(s) 756
MvaI CCWGG 3 cut(s) 50, 211, 335
MvnI CGCG 1 cut(s) 204
NarI GGCGCC 1 cut(s) 524
NciI CCSGG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 477
NdeII GATC 3 cut(s) 290, 582, 703
NlaIII CATG 5 cut(s) 103, 481, 616, 710, 828
NlaIV GGNNCC 5 cut(s) 121, 136, 425, 525, 813
NmeAIII GCCGAG 4 cut(s) 88, 97, 457, 493
PcsI WCGNNNNNNNCGW 3 cut(s) 264, 273, 770
PctI GAATGC 1 cut(s) 756
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 330
PfoI TCCNGGA 1 cut(s) 333
PkrI GCNGC 5 cut(s) 130, 208, 251, 466, 496
PleI GAGTC 2 cut(s) 388, 837
PluTI GGCGCC 1 cut(s) 527
PmaCI CACGTG 1 cut(s) 268
PmlI CACGTG 1 cut(s) 268
PpsI GAGTC 2 cut(s) 388, 837
Ppu21I YACGTR 2 cut(s) 43, 268
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 3 cut(s) 48, 209, 333
PspCI CACGTG 1 cut(s) 268
PspGI CCWGG 3 cut(s) 48, 209, 333
PspN4I GGNNCC 5 cut(s) 121, 136, 425, 525, 813
PspPI GGNCC 1 cut(s) 135
PstI CTGCAG 1 cut(s) 348
PsuI RGATCY 2 cut(s) 290, 582
RsaI GTAC 4 cut(s) 41, 47, 287, 425
RsaNI GTAC 4 cut(s) 40, 46, 286, 424
RseI CAYNNNNRTG 1 cut(s) 823
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 4 cut(s) 588, 660, 714, 807
SatI GCNGC 5 cut(s) 129, 207, 250, 465, 495
Sau3AI GATC 3 cut(s) 290, 582, 703
Sau96I GGNCC 1 cut(s) 135
SchI GAGTC 2 cut(s) 389, 838
ScrFI CCNGG 4 cut(s) 50, 133, 211, 335
SduI GDGCHC 2 cut(s) 267, 342
SfaNI GCATC 2 cut(s) 541, 839
SfcI CTRYAG 1 cut(s) 344
SfoI GGCGCC 1 cut(s) 525
SinI GGWCC 1 cut(s) 135
SmiMI CAYNNNNRTG 1 cut(s) 823
Sse9I AATT 5 cut(s) 639, 670, 742, 804, 834
SsiI CCGC 9 cut(s) 9, 126, 129, 202, 207, 250, 315, 462, 465
SspDI GGCGCC 1 cut(s) 523
SspI AATATT 1 cut(s) 699
SspMI CTAG 1 cut(s) 785
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 4 cut(s) 48, 131, 209, 333
StyI CCWWGG 3 cut(s) 294, 384, 477
TaaI ACNGT 2 cut(s) 39, 148
TaiI ACGT 4 cut(s) 45, 189, 270, 767
TaqI TCGA 4 cut(s) 231, 746, 767, 832
TaqII GACCGA 2 cut(s) 152, 320
TasI AATT 5 cut(s) 639, 670, 742, 804, 834
TatI WGTACW 1 cut(s) 285
TauI GCSGC 4 cut(s) 131, 209, 252, 467
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 4 cut(s) 588, 660, 714, 807
Tru9I TTAA 4 cut(s) 588, 660, 714, 807
TscAI CASTG 1 cut(s) 734
TseI GCWGC 1 cut(s) 494
TspDTI ATGAA 4 cut(s) 88, 419, 651, 807
TspRI CASTG 1 cut(s) 734
VpaK11BI GGWCC 1 cut(s) 135
XapI RAATTY 1 cut(s) 742
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.