pycom15g27870

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008632
Physical Location & Seq
Reverse (-)
23415784 .. 23418396
2613 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGGCACTATCAAGGAAAGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGTCCCAGCGTTAGTGACATTGTGGACAGTTTGGAGGAAAGAGGAATCAACATGCTTTTTGTTTTGGGTGGAAATGGCACACATGCTGGAGCCGATGCAATACACAATGAGTGCCGTAAAAGACGGTTAAGAATGGCTGTAGTTGGTGTTCCGAAAACCATAGACAATGATATTTTGCTGATGGACAAAACTTTTGGTTTCGATACGGCTGTTGAAGAAGCTCAACGGGCAATAAATTCAGCATACATCGAAGCACATAGTGCTTACCATGGCGTTGGAATTGTTAAATTGATGGGTCGTAGCAGTGGGTTCATAGCAATGCATGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTTCCTTTTCAAATACATGGCCCTCATGGTATTTTGCACCATCTGAAATACCTAATTCAAACAAAGGGATCAGCTGTGGTCTGCGTAGCAGAGGGAGCAGGGCAGAATTTAATACAGAAAACCAATGCTACTGATGCATCTGGAAATATGGTATTTGGAGATATTGGCGTGCATATTCAGCAAGAGACAAAAAGGCATTTTAAAGAGGCTGGTGTTCCCGTTGACGTGAAGTATATAGACCCCACGTACATGATCCGTGCATGTCGTGCAAATGCATCTGATGGAATTCTCTGCGCTGTGCTAGGACAAAATGCTGTTCATGGTGCATTTGCTGGATACAGTGGCATCACAGTTGGCACTTGCAACACTCATTATGTATACCTTCCAATTCCAGAGGTCATTTCTCATCCCAGGCTGGTGGATCCTAACAGCCGAATGTGGCATCGTTGCTTGACTTCCACAGGCCAGCCAGATTTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

308

Amino Acids

32.94

Weight (kDa)

7.27

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 3 - 254 3.3e-48 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 819
AclWI GGATC 4 cut(s) 517, 688, 857, 870
AcsI RAATTY 3 cut(s) 307, 547, 726
AcvI CACGTG 1 cut(s) 68
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 1 cut(s) 689
AgsI TTSAA 4 cut(s) 26, 287, 452, 500
AjiI CACGTC 1 cut(s) 667
AjnI CCWGG 2 cut(s) 402, 851
AluBI AGCT 3 cut(s) 51, 293, 515
AluI AGCT 3 cut(s) 51, 293, 515
Alw26I GTCTC 1 cut(s) 620
AlwI GGATC 4 cut(s) 517, 688, 857, 870
AoxI GGCC 2 cut(s) 460, 904
ApoI RAATTY 3 cut(s) 307, 547, 726
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
AspLEI GCGC 1 cut(s) 737
AspS9I GGNCC 2 cut(s) 74, 461
AvaII GGWCC 1 cut(s) 74
AxyI CCTNAGG 1 cut(s) 438
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BamHI GGATCC 1 cut(s) 862
BbrPI CACGTG 1 cut(s) 68
BccI CCATC 4 cut(s) 247, 358, 489, 716
BceAI ACGGC 2 cut(s) 171, 294
BciT130I CCWGG 2 cut(s) 404, 853
BciVI GTATCC 1 cut(s) 770
BcoDI GTCTC 1 cut(s) 620
BfaI CTAG 3 cut(s) 408, 743, 922
BfmI CTRYAG 1 cut(s) 210
BfuI GTATCC 1 cut(s) 770
Bme1390I CCNGG 2 cut(s) 404, 853
Bme18I GGWCC 1 cut(s) 74
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 2 cut(s) 74, 461
BmiI GGNNCC 4 cut(s) 76, 163, 444, 864
BmrFI CCNGG 2 cut(s) 404, 853
BmsI GCATC 7 cut(s) 157, 407, 565, 587, 725, 795, 892
BpmI CTGGAG 1 cut(s) 180
BsaAI YACGTR 2 cut(s) 68, 687
BsaJI CCNNGG 3 cut(s) 340, 402, 851
Bse21I CCTNAGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 396
BseBI CCWGG 2 cut(s) 404, 853
BseDI CCNNGG 3 cut(s) 340, 402, 851
BseGI GGATG 2 cut(s) 398, 847
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 77
BshFI GGCC 2 cut(s) 462, 906
BslFI GGGAC 1 cut(s) 60
BsmAI GTCTC 1 cut(s) 620
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 2 cut(s) 462, 906
Bsp143I GATC 3 cut(s) 509, 693, 862
Bsp19I CCATGG 1 cut(s) 340
BspANI GGCC 2 cut(s) 462, 906
BspCNI CTCAG 1 cut(s) 430
BspLI GGNNCC 4 cut(s) 76, 163, 444, 864
BspPI GGATC 4 cut(s) 517, 688, 857, 870
BsrDI GCAATG 1 cut(s) 396
BssECI CCNNGG 3 cut(s) 340, 402, 851
BssMI GATC 3 cut(s) 509, 693, 862
BssNAI GTATAC 1 cut(s) 820
BssT1I CCWWGG 1 cut(s) 340
Bst1107I GTATAC 1 cut(s) 820
Bst2UI CCWGG 2 cut(s) 404, 853
Bst4CI ACNGT 4 cut(s) 101, 198, 782, 793
BstAPI GCANNNNNTGC 3 cut(s) 332, 395, 707
BstBAI YACGTR 2 cut(s) 68, 687
BstC8I GCNNGC 3 cut(s) 396, 611, 908
BstDEI CTNAG 1 cut(s) 438
BstDSI CCRYGG 1 cut(s) 340
BstF5I GGATG 2 cut(s) 398, 847
BstHHI GCGC 1 cut(s) 737
BstKTI GATC 3 cut(s) 512, 696, 865
BstMAI GTCTC 1 cut(s) 620
BstMBI GATC 3 cut(s) 509, 693, 862
BstMWI GCNNNNNNNGC 8 cut(s) 299, 332, 395, 404, 536, 575, 619, 707
BstNI CCWGG 2 cut(s) 404, 853
BstNSI RCATGY 4 cut(s) 127, 158, 398, 705
BstSCI CCNGG 2 cut(s) 402, 851
BstSFI CTRYAG 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 862
BstXI CCANNNNNNTGG 2 cut(s) 347, 859
BstYI RGATCY 1 cut(s) 862
BstZ17I GTATAC 1 cut(s) 820
Bsu36I CCTNAGG 1 cut(s) 438
BsuI GTATCC 1 cut(s) 770
BsuRI GGCC 2 cut(s) 462, 906
BtgI CCRYGG 1 cut(s) 340
BtrI CACGTC 1 cut(s) 667
BtsCI GGATG 2 cut(s) 398, 847
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 382, 787
Cac8I GCNNGC 3 cut(s) 396, 611, 908
CfoI GCGC 1 cut(s) 737
Cfr13I GGNCC 2 cut(s) 74, 461
Csp6I GTAC 1 cut(s) 688
CspCI CAANNNNNGTGG 1 cut(s) 35
CviAII CATG 9 cut(s) 124, 155, 341, 395, 458, 467, 691, 702, 761
CviQI GTAC 1 cut(s) 688
DdeI CTNAG 1 cut(s) 438
DpnI GATC 3 cut(s) 511, 695, 864
DpnII GATC 3 cut(s) 509, 693, 862
DraI TTTAAA 1 cut(s) 643
DraIII CACNNNGTG 1 cut(s) 332
Eco130I CCWWGG 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 74
Eco72I CACGTG 1 cut(s) 68
Eco81I CCTNAGG 1 cut(s) 438
EcoO109I RGGNCCY 1 cut(s) 74
EcoRI GAATTC 1 cut(s) 726
EcoRII CCWGG 2 cut(s) 402, 851
EcoT14I CCWWGG 1 cut(s) 340
EcoT22I ATGCAT 4 cut(s) 396, 400, 580, 718
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 9 cut(s) 127, 158, 344, 398, 461, 470, 694, 705, 764
FaqI GGGAC 1 cut(s) 60
FatI CATG 9 cut(s) 123, 154, 340, 394, 457, 466, 690, 701, 760
FauNDI CATATG 1 cut(s) 425
FblI GTMKAC 1 cut(s) 819
FokI GGATG 2 cut(s) 385, 834
FspBI CTAG 3 cut(s) 408, 743, 922
GlaI GCGC 1 cut(s) 736
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 2 cut(s) 462, 906
HhaI GCGC 1 cut(s) 737
Hin1II CATG 9 cut(s) 127, 158, 344, 398, 461, 470, 694, 705, 764
Hin6I GCGC 1 cut(s) 735
HinP1I GCGC 1 cut(s) 735
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 1 cut(s) 49
HinfI GANTC 1 cut(s) 117
Hpy166II GTNNAC 4 cut(s) 97, 413, 664, 820
Hpy188I TCNGA 3 cut(s) 225, 486, 721
Hpy188III TCNNGA 2 cut(s) 582, 833
Hpy8I GTNNAC 4 cut(s) 97, 413, 664, 820
HpyAV CCTTC 1 cut(s) 833
HpyCH4III ACNGT 4 cut(s) 101, 198, 782, 793
HpyCH4IV ACGT 3 cut(s) 67, 666, 686
HpyF10VI GCNNNNNNNGC 8 cut(s) 299, 332, 395, 404, 536, 575, 619, 707
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 3 cut(s) 67, 666, 686
Hsp92II CATG 9 cut(s) 127, 158, 344, 398, 461, 470, 694, 705, 764
HspAI GCGC 1 cut(s) 735
Kzo9I GATC 3 cut(s) 509, 693, 862
LmnI GCTCC 2 cut(s) 161, 536
LweI GCATC 7 cut(s) 157, 407, 565, 587, 725, 795, 892
MaeI CTAG 3 cut(s) 408, 743, 922
MaeII ACGT 3 cut(s) 67, 666, 686
MaeIII GTNAC 1 cut(s) 86
MalI GATC 3 cut(s) 511, 695, 864
MboI GATC 3 cut(s) 509, 693, 862
MboII GAAGA 1 cut(s) 299
MflI RGATCY 1 cut(s) 862
MluCI AATT 8 cut(s) 307, 351, 359, 417, 495, 547, 726, 828
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 8 cut(s) 65, 100, 107, 433, 474, 526, 640, 829
Mph1103I ATGCAT 4 cut(s) 396, 400, 580, 718
MseI TTAA 4 cut(s) 200, 357, 551, 642
MslI CAYNNNNRTG 2 cut(s) 389, 689
MspA1I CMGCKG 1 cut(s) 515
MspR9I CCNGG 2 cut(s) 404, 853
MvaI CCWGG 2 cut(s) 404, 853
MwoI GCNNNNNNNGC 8 cut(s) 299, 332, 395, 404, 536, 575, 619, 707
NcoI CCATGG 1 cut(s) 340
NdeI CATATG 1 cut(s) 425
NdeII GATC 3 cut(s) 509, 693, 862
NlaIII CATG 9 cut(s) 127, 158, 344, 398, 461, 470, 694, 705, 764
NlaIV GGNNCC 4 cut(s) 76, 163, 444, 864
NmuCI GTSAC 1 cut(s) 86
NsiI ATGCAT 4 cut(s) 396, 400, 580, 718
NspI RCATGY 4 cut(s) 127, 158, 398, 705
PaeI GCATGC 1 cut(s) 398
PfeI GAWTC 1 cut(s) 117
PmaCI CACGTG 1 cut(s) 68
PmlI CACGTG 1 cut(s) 68
Ppu21I YACGTR 2 cut(s) 68, 687
PpuMI RGGWCCY 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 74
Psp6I CCWGG 2 cut(s) 402, 851
PspCI CACGTG 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 402, 851
PspN4I GGNNCC 4 cut(s) 76, 163, 444, 864
PspPI GGNCC 2 cut(s) 74, 461
PspPPI RGGWCCY 1 cut(s) 74
PsrI GAACNNNNNNTAC 2 cut(s) 204, 236
PsuI RGATCY 1 cut(s) 862
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 1 cut(s) 689
RsaNI GTAC 1 cut(s) 688
RseI CAYNNNNRTG 2 cut(s) 389, 689
SaqAI TTAA 4 cut(s) 200, 357, 551, 642
Sau3AI GATC 3 cut(s) 509, 693, 862
Sau96I GGNCC 2 cut(s) 74, 461
ScrFI CCNGG 2 cut(s) 404, 853
SfaNI GCATC 7 cut(s) 157, 407, 565, 587, 725, 795, 892
SfcI CTRYAG 1 cut(s) 210
SinI GGWCC 1 cut(s) 74
SmiMI CAYNNNNRTG 2 cut(s) 389, 689
SphI GCATGC 1 cut(s) 398
Sse9I AATT 8 cut(s) 307, 351, 359, 417, 495, 547, 726, 828
SspMI CTAG 3 cut(s) 408, 743, 922
StyD4I CCNGG 2 cut(s) 402, 851
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 4 cut(s) 101, 198, 782, 793
TaiI ACGT 3 cut(s) 70, 669, 689
TaqI TCGA 2 cut(s) 273, 321
TasI AATT 8 cut(s) 307, 351, 359, 417, 495, 547, 726, 828
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 4 cut(s) 200, 357, 551, 642
Tru9I TTAA 4 cut(s) 200, 357, 551, 642
TscAI CASTG 2 cut(s) 382, 787
TseFI GTSAC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 3 cut(s) 23, 373, 749
TspGWI ACGGA 1 cut(s) 686
TspRI CASTG 2 cut(s) 382, 787
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 3 cut(s) 307, 547, 726
XceI RCATGY 4 cut(s) 127, 158, 398, 705
XmiI GTMKAC 1 cut(s) 819
XspI CTAG 3 cut(s) 408, 743, 922
Zsp2I ATGCAT 4 cut(s) 396, 400, 580, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.